journal
MENU ▼
Read by QxMD icon Read
search

BioTechniques

journal
https://www.readbyqxmd.com/read/29692182/a-microfluidic-based-lid-device-for-conventional-cell-culture-dishes-to-automatically-control-oxygen-level
#1
Seung Yeob Lee, Sung Yang
Most conventional hypoxic cell culture systems undergo reoxygenation during experimental manipulations, resulting in undesirable effects including the reduction of cell viability. A lid device was developed herein for conventional cell culture dishes to resolve this limitation. The integration of multilayered microfluidic channels inside a thin membrane was designed to prevent the reoxygenation caused by reagent infusion and automatically control the oxygen level. The experimental data clearly show the reducibility of the dissolved oxygen in the infusing reagent and the controllability of the oxygen level inside the dish...
April 25, 2018: BioTechniques
https://www.readbyqxmd.com/read/29673256/a-novel-easy-and-rapid-method-for-constructing-yeast-two-hybrid-vectors-using-in-fusion-technology
#2
Deshui Yu, Libing Liao, Ju Zhang, Yi Zhang, Kedong Xu, Kun Liu, Xiaoli Li, Guangxuan Tan, Ran Chen, Yulu Wang, Xia Liu, Xuan Zhang, Xiaomeng Han, Zhangkun Wei, Chengwei Li
Yeast two-hybrid systems are powerful tools for analyzing interactions between proteins. Vector construction is an essential step in yeast two-hybrid experiments, which require bait and prey plasmids. In this study, we modified the multiple cloning site sequence of the yeast plasmid pGADT7 by site-directed mutagenesis PCR to generate the pGADT7-In vector, which result in an easy and rapid method for constructing yeast two-hybrid vectors using the In-Fusion cloning technique. This method has three key advantages: only one pair of primers and one round of PCR are needed to generate bait and prey plasmids for each gene; it is restriction endonuclease- and ligase-independent and it is fast and easily performed...
April 19, 2018: BioTechniques
https://www.readbyqxmd.com/read/29661021/dextran-sodium-sulfate-for-colitis-animal-model-research
#3
(no author information available yet)
No abstract text is available yet for this article.
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661020/bio-spotlight-citations
#4
Kristie Nybo, Danielle Gerhard
No abstract text is available yet for this article.
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661019/real-time-assessment-of-apoptosis-and-necrosis
#5
(no author information available yet)
No abstract text is available yet for this article.
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661018/improved-protein-blotting-efficiency-with-new-fast-wet-blotting-technology
#6
(no author information available yet)
No abstract text is available yet for this article.
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661017/a-novel-orange-colored-bimolecular-fluorescence-complementation-bifc-assay-using-monomeric-kusabira-orange-protein
#7
Yuta Fujii, Ayaka Yoshimura, Yutaka Kodama
The bimolecular fluorescence complementation (BiFC) assay was developed as a tool for the visualization of protein-protein interactions in living cells. To date, many types of BiFC systems with distinct colors have been developed. Most of the colors in the visible spectrum have been used in BiFC assays, with the exception of orange. In this study, we developed an orange-colored BiFC system using the Kusabira-Orange (KO) protein from the stony coral Fungia concinna. To obtain bright BiFC fluorescence, we compared fluorescence intensities of two monomeric KO variants (mKO1 and mKO2) and identified mKO2 as brighter than mKO1...
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661016/in-fusion-%C3%A2-cloning-accuracy-not-background
#8
(no author information available yet)
No abstract text is available yet for this article.
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661015/seeing-is-believing-structures-and-functions-of-biological-molecules
#9
Nawsheen Boodhun
The ability to elucidate the structure and function of biological molecules holds great importance in a variety of domains. This includes prospects to tackle public health concerns, identification of new drug targets and therapeutic agents. In this issue of Tech News, Nawsheen Boodhun explores techniques used to understand macromolecules.
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661014/a-novel-protocol-for-generating-intact-whole-head-spider-cephalothorax-tissue-sections
#10
Skye M Long
The diversity of spider behavior and sensory systems provides an excellent opportunity for comparative studies of the relationship between the brain and behavior. However, the morphology of spiders poses a challenge for histologists since the spider cephalothorax contains heterogeneous tissues and has both tough external and internal sclerotized regions. Unlike the heads of insects, the cephalothorax is highly pressurized, which can cause tissues to shift during processing and can reduce tissue cohesion in thick sections...
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661013/to-find-what-s-unique-stop-averaging
#11
Meenakshi Prabhune
Written by Meenakshi Prabhune, PhD Will recent advances in single-cell analysis methods help us better understand and improve human health?
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661012/using-synthetic-oligonucleotides-as-standards-in-probe-based-qpcr
#12
Jillian Conte, Margret J Potoczniak, Shanan S Tobe
Real-time PCR (qPCR) is widely used in the life sciences. For quantifying DNA, a standard curve is required. Common methods for standard development are time consuming, costly, necessitate a specific skill set, and pose a contamination risk. Using a targeted synthetic oligonucleotide, such as a gBlocks® Gene Fragment, overcomes these drawbacks and provides researchers an accurate and quick solution to standard development. Here, we demonstrate that using a gBlocks fragment as a standard provides comparable sensitivity, reliability, and assay performance to a purified amplicon standard...
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661011/protein-extraction-from-human-anagen-head-hairs-1-millimeter-or-less-in-total-length
#13
Traci L Carlson, Mehdi Moini, Brian A Eckenrode, Brent M Allred, Joseph Donfack
A simple method for extracting protein from human anagen (i.e., actively growing hair stage) head hairs was developed in this study for cases of limited sample availability and/or studies of specific micro-features within a hair. The distinct feature segments of the hair from one donor were divided lengthwise (i.e., each of ∼200-400 μm) and then pooled for three individual hairs to form a total of eight composite hair samples (i.e., each of ∼1 mm or less in total length). The proteins were extracted, digested using trypsin, and characterized via nano-flow liquid chromatography tandem-mass spectrometry (nLC-MS/MS)...
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661010/development-of-a-cost-effective-and-robust-alphascreen-%C3%A2-platform-for-application
#14
John R Veloria, Ashwini K Devkota, Eun Jeong Cho, Kevin N Dalby
The use of AlphaScreen® detection has allowed researchers to examine a wide variety of molecular interactions for use in high-throughput screening. However, the cost of Alpha reagents can often be prohibitory for extended screening campaigns or for young investigators with limited funding. To reduce assay costs, many labs have focused on miniaturization, while there have been limited efforts to scale down Alpha reagents. Thus, we describe the optimization of an AlphaScreen detection platform by systematically reducing the Alpha reagents down to 2...
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29661009/to-blind-or-not-to-blind-the-search-for-a-perfect-peer-review-methodology
#15
Francesca Lake
No abstract text is available yet for this article.
April 2018: BioTechniques
https://www.readbyqxmd.com/read/29570443/single-step-production-of-cas9-mrna-for-zygote-injection
#16
Bethany K Redel, Benjamin P Beaton, Lee D Spate, Joshua A Benne, Stephanie L Murphy, Chad W O'Gorman, Anna M Spate, Randall S Prather, Kevin D Wells
Production of Cas9 mRNA in vitro typically requires the addition of a 5´ cap and 3´ polyadenylation. A plasmid was constructed that harbored the T7 promoter followed by the EMCV IRES and a Cas9 coding region. We hypothesized that the use of the metastasis associated lung adenocarcinoma transcript 1 (Malat1) triplex structure downstream of an IRES/Cas9 expression cassette would make polyadenylation of in vitro produced mRNA unnecessary. A sequence from the mMalat1 gene was cloned downstream of the IRES/Cas9 cassette described above...
March 1, 2018: BioTechniques
https://www.readbyqxmd.com/read/29570442/improved-spot-morphology-for-printed-glycan-arrays
#17
Navakouski Maksim, Shilova Nadezhda, Khasbiullina Nailya, Feofanov Alexey, Pudova Elena, Chen Kowa, Blixt Ola, Bovin Nicolai
Despite considerable success studying glycan-binding proteins using printed glycan arrays (PGAs), unambiguous quantitation of spot intensities by fluorescent readers remains a challenge. The main obstacles are the varying spot shape and size and in-spot fluorescence distribution caused by uneven drying of the printed drops. Two methods have been suggested for solving this problem: using polymeric glycoconjugates, which makes it possible to equalize the physicochemical properties (hydrophobicity, charge, and size) of different glycans, and applying a glycan solution on a slide coated with a thin oil mask, which hinders evaporation of the drop...
March 1, 2018: BioTechniques
https://www.readbyqxmd.com/read/29570441/restricted-exchange-microenvironments-for-cell-culture
#18
Jan H Hoh, Jeffrey L Werbin, William F Heinz
Metabolite diffusion in tissues produces gradients and heterogeneous microenvironments that are not captured in standard 2D cell culture models. Here we describe restricted exchange environment chambers (REECs) in which diffusive gradients are formed and manipulated on length scales approximating those found in vivo. In REECs, cells are grown in 2D in an asymmetric chamber (<50 μL) formed between a coverglass and a glass bottom cell culture dish separated by a thin (~100 μm) gasket. Diffusive metabolite exchange between the chamber and bulk media occurs through one or more openings micromachined into the coverglass...
March 1, 2018: BioTechniques
https://www.readbyqxmd.com/read/29570440/building-the-future-from-3d-blueprints
#19
Meenakshi Prabhune
3D printed biomaterials are increasingly used in cell cultures and drug screens. Given the ease of creating artificial tissues, will this technique revolutionize biomedicine and organ implants in the future?
March 1, 2018: BioTechniques
https://www.readbyqxmd.com/read/29570439/taking-it-to-the-gut
#20
Sarah Webb
SARAH WEBB EXAMINES HOW GASTROINTESTINAL ORGANOIDS ARE TRANSFORMING OUR UNDERSTANDING OF BIOLOGY AND HELPING PATIENTS.
March 1, 2018: BioTechniques
journal
journal
28617
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"