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Systematic and Applied Microbiology

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https://www.readbyqxmd.com/read/28094097/diverse-genotypes-of-bradyrhizobium-nodulate-herbaceous-chamaecrista-moench-fabaceae-caesalpinioideae-species-in-brazil
#1
José Miguel Ferreira Dos Santos, Patricia Alves Casaes Alves, Verônica Cordeiro Silva, Mariana Ferreira Kruschewsky Rhem, Euan K James, Eduardo Gross
The genus Chamaecrista comprises more than 330 species which are mainly distributed across tropical America, especially in Brazil (256 spp.), the main center of radiation. In this study, nodulation of herbaceous Chamaecrista species that are commonly found growing in different vegetation types in the north eastern Brazilian state of Bahia was assessed together with the diversity of rhizobia isolated from their root nodules. Genetic characterization of the isolates was performed using molecular markers to examine the phylogeny of their "core" (16S rRNA, ITS, recA, glnII, dnaK and gyrB) and symbiosis-related (nifH, nodC) genomes...
January 10, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28081924/physiological-and-gene-expression-responses-to-nitrogen-regimes-and-temperatures-in-mastigocladus-sp-strain-chp1-a-predominant-thermotolerant-cyanobacterium-of-hot-springs
#2
M Estrella Alcamán, Jaime Alcorta, Birgitta Bergman, Mónica Vásquez, Martin Polz, Beatriz Díez
Cyanobacteria are widely distributed primary producers with significant implications for the global biogeochemical cycles of carbon and nitrogen. Diazotrophic cyanobacteria of subsection V (Order Stigonematales) are particularly ubiquitous in photoautotrophic microbial mats of hot springs. The Stigonematal cyanobacterium strain CHP1 isolated from the Porcelana hot spring (Chile) was one of the major contributors of the new nitrogen through nitrogen fixation. Further morphological and genetic characterization verified that the strain CHP1 belongs to Stigonematales, and it formed a separate clade together with other thermophiles of the genera Fischerella and Mastigocladus...
December 28, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28081923/analysis-of-rhizobial-endosymbionts-of-vicia-lathyrus-and-trifolium-species-used-to-maintain-mountain-firewalls-in-sierra-nevada-national-park-south-spain
#3
Pablo J Villadas, Ana V Lasa, Pilar Martínez-Hidalgo, José David Flores-Félix, Eustoquio Martínez-Molina, Nicolás Toro, Encarna Velázquez, Manuel Fernández-López
Forest fires lead to the annual disappearance of many natural formations that require the creation of firewall areas. They can be maintained by enriching their pastures with attractive plants for grazing livestock, mainly legumes, which have a high protein content and low dependence on N fertilizers due to their ability to establish nitrogen-fixing symbiosis with rhizobia. In this study, the rhizobia isolated from the nodules of six legumes from the genera Vicia, Lathyrus and Trifolium were analysed in a firewall zone established in Lanjarón (Granada) close to the Sierra Nevada National Park (Spain)...
December 28, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28063627/competition-between-rhizobia-under-different-environmental-conditions-affects-the-nodulation-of-a-legume
#4
Zhao Jun Ji, Hui Yan, Qing Guo Cui, En Tao Wang, Wen Feng Chen, Wen Xin Chen
Mutualistic symbiosis and nitrogen fixation of legume rhizobia play a key role in ecological environments. Although many different rhizobial species can form nodules with a specific legume, there is often a dominant microsymbiont, which has the highest nodule occupancy rates, and they are often known as the "most favorable rhizobia". Shifts in the most favorable rhizobia for a legume in different geographical regions or soil types are not well understood. Therefore, in order to explore the shift model, an experiment was designed using successive inoculations of rhizobia on one legume...
December 27, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28040300/neptuniibacter-pectenicola-sp-nov-and-neptuniibacter-marinus-sp-nov-two-novel-species-isolated-from-a-great-scallop-pecten-maximus-hatchery-in-norway-and-emended-description-of-the-genus-neptuniibacter
#5
Ana L Diéguez, Sabela Balboa, Thorolf Magnesen, Jesús L Romalde
Nine isolates obtained from a great scallop hatchery in Norway were characterized using a polyphasic approach. Strains were Gram-negative, aerobic and motile rods with oxidative metabolism. Phylogenetic analysis based on the sequences of 16S rRNA and rpoB genes showed that these strains formed two different groups associated with members of the genus Neptuniibacter. DNA-DNA hybridization (DDH) and Average Nucleotide Identity (ANI) demonstrated that the isolates constituted two novel species of this genus, which can be phenotypically differentiated from their closest relatives...
December 21, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28057375/description-of-a-new-anaerobic-thermophilic-bacterium-thermoanaerobacterium-butyriciformans-sp-nov
#6
G López, S J Cañas-Duarte, A M Pinzón-Velasco, N E Vega-Vela, M Rodríguez, S Restrepo, S Baena
Strain USBA-019(T), an anaerobic and thermophilic strain, was identified as a new member of the genus Thermoanaerobacterium. USBA-019(T) cells are gram-positive, strictly anaerobic, thermophilic, chemoorganotrophic, moderately acidophilic, non-motile, endospore-forming, slightly curved, and rod-shaped. Cells measure 0.4×3.0-7.0μm. Optimal growth occurs at 50-55°C (35-65°C). Optimum pH is 5.0-5.5 (4.0-8.5). Thiosulfate, elemental sulfur and nitrate were utilized as electron acceptors. Fermentation of glucose, lactose, cellobiose, galactose, arabinose, xylose, starch and xylan primarily produced acetate and butyrate...
December 16, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27939530/diverse-mesorhizobium-bacteria-nodulate-native-astragalus-and-oxytropis-in-arctic-and-subarctic-areas-in-eurasia
#7
Osei Yaw Ampomah, Seyed Abdollah Mousavi, Kristina Lindström, Kerstin Huss-Danell
Rhizobia nodulating native Astragalus and Oxytropis spp. in Northern Europe are not well-studied. In this study, we isolated bacteria from nodules of four Astragalus spp. and two Oxytropis spp. from the arctic and subarctic regions of Sweden and Russia. The phylogenetic analyses were performed by using sequences of three housekeeping genes (16S rRNA, rpoB and recA) and two accessory genes (nodC and nifH). The results of our multilocus sequence analysis (MLSA) of the three housekeeping genes tree showed that all the 13 isolates belonged to the genus Mesorhizobium and were positioned in six clades...
November 30, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27931747/genetic-diversity-and-population-structure-of-food-borne-staphylococcus-carnosus-strains
#8
Anne Bückle Née Müller, Markus Kranz, Herbert Schmidt, Agnes Weiss
The species Staphylococcus carnosus is a non-pathogenic representative of the coagulase negative staphylococci. Specific strains are applied as meat starter cultures. The species consists of two subspecies, S. carnosus ssp. carnosus and S. carnosus ssp. utilis. In order to place S. carnosus strains, characterized in former studies, in a genetic background that allows a typing of candidates for starter cultures, a Multilocus Sequence Typing (MLST) scheme was developed. Internal fragments of the genes tpiA, xprT, dat, gmk, glpK, narG, cstA, encoding triosephosphate isomerase, xanthine phosphoribosyltransferase, d-amino acid aminotransferase, guanylate kinase, glycerol kinase, the α-chain of the respiratory nitrate reductase, and a carbon starvation protein where chosen...
November 30, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27939529/exploring-the-biodiversity-of-two-groups-of-oenococcus-oeni-isolated-from-grape-musts-and-wines-are-they-equally-diverse
#9
Liz Erika Cruz-Pio, Marta Poveda, María Rosa Alberto, Sergi Ferrer, Isabel Pardo
One hundred and four Oenococcus oeni isolates were characterised by the carbohydrate fermentation (CH) profile and DNA fingerprinting. Forty-four isolates came from grape must, and 60 from wines sampled at the end of alcoholic fermentation or during malolactic fermentation. The grape must isolates fermented more CH than the wine isolates. In genotypical terms, no clear boundary between grape must and wine isolates was found. Diversities were deduced by considering the isolates of grape must and of wine separately and jointly...
November 24, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27931748/lentil-lens-culinaris-medik-nodulates-with-genotypically-and-phenotypically-diverse-rhizobia-in-ethiopian-soils
#10
Wondwosen Tena, Endalkachew Wolde-Meskel, Tulu Degefu, Fran Walley
Forty-eight lentil-nodulating rhizobia were isolated from soil samples collected from diverse agro-ecological locations in Ethiopia, and characterized based on 76 phenotypic traits. Furthermore, 26 representative strains were selected and characterized using multilocus sequence analyses (MLSA) of core (16S rRNA, recA, atpD, glnII and gyrB) and symbiotic (nodA and nifH) genes. Numerical analysis of phenotypic characteristics showed that the 48 test strains fell into three major distinct clusters. The phylogenetic trees based on 16S rRNA genes showed that they belong to the Rhizobium genus...
November 24, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27914738/wolbachia-strains-in-cryptic-species-of-the-anastrepha-fraterculus-complex-diptera-tephritidae-along-the-neotropical-region
#11
Leandro F Prezotto, André L P Perondini, Vicente Hernández-Ortiz, Celso L Marino, Denise Selivon
Infection by Wolbachia was described previously in eleven species of Anastrepha fruit flies some of which are important pests of fruticulture. One such species is the nominal Anastrepha fraterculus, the South American fruit fly, which actually comprises a complex of cryptic species. The suggestions of using Wolbachia for the control of these pest species, make imperative a more precise characterization of the existing strains of the bacteria. In this study, population samples of the A. fraterculus complex from Brazil, Argentina, Peru, Ecuador, Colombia, Guatemala and Mexico were analyzed for Wolbachia infection...
November 24, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27913074/draft-genome-and-description-of-orrella-dioscoreae-gen-nov-sp-nov-a-new-species-of-alcaligenaceae-isolated-from-leaf-acumens-of-dioscorea-sansibarensis
#12
Aurelien Carlier, Margo Cnockaert, Linda Fehr, Peter Vandamme, Leo Eberl
Dioscorea sansibarensis is a monocot species of inedible yam native of tropical Africa and Madagascar. Leaves of D. sansibarensis feature prominent acumen glands that are densely colonized by bacteria. We describe here the isolation and characterization of bacteria from leaf glands of D. sansibarensis specimens obtained from various botanical gardens. Phylogenetic and phenotypic characterizations indicate that the isolates belong to a novel genus and species of the Alcaligenaceae family for which we propose the name Orrella dioscoreae gen...
November 24, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27816261/detection-isolation-and-characterization-of-fusobacterium-gastrosuis-sp-nov-colonizing-the-stomach-of-pigs
#13
C De Witte, B Flahou, R Ducatelle, A Smet, E De Bruyne, M Cnockaert, B Taminiau, G Daube, P Vandamme, F Haesebrouck
Nine strains of a novel Fusobacterium sp. were isolated from the stomach of 6-8 months old and adult pigs. The isolates were obligately anaerobic, although they endured 2h exposure to air. Phylogenetic analysis based on 16S rRNA and gyrase B genes demonstrated that the isolates showed high sequence similarity with Fusobacterium mortiferum, Fusobacterium ulcerans, Fusobacterium varium, Fusobacterium russii and Fusobacterium necrogenes, but formed a distinct lineage in the genus Fusobacterium. Comparative analysis of the genome of the type strain of this novel Fusobacterium sp...
October 24, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27776911/genomic-characterization-of-a-fructophilic-bee-symbiont-lactobacillus-kunkeei-reveals-its-niche-specific-adaptation
#14
Shintaro Maeno, Yasuhiro Tanizawa, Yu Kanesaki, Eri Kubota, Himanshu Kumar, Leon Dicks, Seppo Salminen, Junichi Nakagawa, Masanori Arita, Akihito Endo
Lactobacillus kunkeei is classified as a sole obligate fructophilic lactic acid bacterium that is found in fructose-rich niches, including the guts of honeybees. The species is differentiated from other lactobacilli based on its poor growth with glucose, enhanced growth in the presence of oxygen and other electron acceptors, and production of high concentrations of acetate from the metabolism of glucose. These characteristics are similar to phylogenetically distant Fructobacillus spp. In the present study, the genomic structure of L...
December 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27771162/multi-locus-sequence-analysis-and-symbiotic-characterization-of-novel-ensifer-strains-nodulating-tephrosia-spp-in-the-indian-thar-desert
#15
Nisha Tak, Esha Awasthi, Garima Bissa, Raju Ram Meghwal, Euan K James, Janet S Sprent, Hukam S Gehlot
Phylogenetically diverse Ensifer strains associated with five species of Tephrosia growing in alkaline soils of semi-arid regions of the Thar Desert were characterized using multi locus sequence analysis. Based on 16S rRNA and four protein-coding housekeeping gene (recA, atpD, glnII and dnaK) sequences, the Tephrosia-Ensifer strains were genetically different from the type strains of Ensifer saheli, Ensifer kostiensis, Ensifer terangae (African origin) and Ensifer psoraleae (Asiatic origin). One strain, Ensifer sp...
December 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27745752/varibaculum-anthropi-sp-nov-represented-by-three-genetically-different-genomovars-isolated-from-clinical-material-and-emended-description-of-the-genus-varibaculum
#16
Stefanie P Glaeser, Swapnil Doijad, Muaz Hijazin, Trinad Chakraborty, Enevold Falsen, Val Hall, Peter Kämpfer
During the years 1994-2011 five strictly anaerobic, Gram-stain-positive, diphtheroid bacteria (strains CCUG 31793(T), CCUG 44221, CCUG 61255, CCUG 45114, and CCUG 44993) were isolated from different clinical samples in Sweden and the United Kingdom. Comparative analysis of 16S rRNA gene sequences showed that the five strains shared 99-100% 16S rRNA gene sequence similarity among each other and 98.3-98.6% sequence similarity to Varibaculum cambriense DSM 15806(T). Genomic fingerprint patterns generated with ERIC-, BOX-, and RAPD-PCR, and whole genome sequence (WGS) based comparison by in silico DNA-DNA hybridization (isDDH), average nucleotide identity (ANI) analysis and six housekeeping gene (atpA, rpoB, pgi, metG, gltA and gyrA) based multilocus sequence analysis (MLSA) showed that the strains could be differentiated from V...
December 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27729171/discrimination-and-divergence-among-lactobacillus-plantarum-group-lpg-isolates-with-reference-to-their-probiotic-functionalities-from-vegetable-origin
#17
Sundru Manjulata Devi, Subramanian Aishwarya, Prakash M Halami
The present study was aimed to evaluate the diversity and probiotic properties of Lactobacillus plantarum-group cultures from vegetable origin. First, genotypic diversity of L. plantarum (n=34) was achieved by PCR of Random Amplified Polymorphic DNA and recA gene-specific multiplex PCR. The isolates were segregated into five groups namely, Lactobacillus pentosus, Lactobacillus paraplantarum, Lactobacillus arizonensis, Lactobacillus plantarum subsp. plantarum and argentoratensis. Further discrimination was achieved by restriction fragment length polymorphism of probiotic adhesion genes viz...
December 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27726901/phylogenomic-re-assessment-of-the-thermophilic-genus-geobacillus
#18
Habibu Aliyu, Pedro Lebre, Jochen Blom, Don Cowan, Pieter De Maayer
Geobacillus is a genus of Gram-positive, aerobic, spore-forming obligate thermophiles. The descriptions and subsequent affiliations of the species in the genus have mostly been based on polyphasic taxonomy rules that include traditional sequence-based methods such as DNA-DNA hybridization and comparison of 16S rRNA gene sequences. Currently, there are fifteen validly described species within the genus. The availability of whole genome sequences has provided an opportunity to validate and/or re-assess these conventional estimates of genome relatedness...
December 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27720237/novel-volatiles-of-skin-borne-bacteria-inhibit-the-growth-of-gram-positive-bacteria-and-affect-quorum-sensing-controlled-phenotypes-of-gram-negative-bacteria
#19
Marie Chantal Lemfack, Srinivasa Rao Ravella, Nicola Lorenz, Marco Kai, Kirsten Jung, Stefan Schulz, Birgit Piechulla
The skin microbiota is import for body protection. Here we present the first comprehensive analysis of the volatile organic compound (VOC) profiles of typical skin-resident corynebacterial and staphylococcal species. The VOC profile of Staphylococcus schleiferi DSMZ 4807 was of particular interest as it is dominated by two compounds, 3-(phenylamino)butan-2-one and 3-(phenylimino)butan-2-one (schleiferon A and B, respectively). Neither of these has previously been reported from natural sources. Schleiferon A and B inhibited the growth of various Gram-positive species and affected two quorum-sensing-dependent phenotypes - prodigiosin accumulation and bioluminescence - of Gram-negative bacteria...
December 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27712916/mesorhizobium-olivaresii-sp-nov-isolated-from-lotus-corniculatus-nodules
#20
María J Lorite, José David Flores-Félix, Álvaro Peix, Juan Sanjuán, Encarna Velázquez
In this study four Mesorhizobium strains isolated from Lotus corniculatus nodules in Granada (Spain) were characterized. Their 16S rRNA gene sequences were closely related to those of M. albiziae LMG 23507(T) and M. chacoense Pr5(T) showing 99.4 and 99.2% similarity values, respectively. The analysis of concatenated rpoB, recA, atpD and glnII genes showed they formed a cluster with internal similarities higher than 97%. The closest species also were M. albiziae LMG 23507(T) and M. chacoense Pr5(T) showing similarity values lower than 92% in rpoB, recA and glnII genes and lower than 96...
December 2016: Systematic and Applied Microbiology
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