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Cell Biochemistry and Function

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https://www.readbyqxmd.com/read/28370084/trpa1-contributed-to-the-neuropathic-pain-induced-by-docetaxel-treatment
#1
Kun Huang, Donglin Bian, Bin Jiang, Qixi Zhai, Ningning Gao, Ruiying Wang
Peripheral mechanical neuropathic pain is a serious side effect of docetaxel chemotherapy for cancer. However, the underlying mechanism for this side effect is unknown. In the present study, we found that docetaxel treatment induced mechanical allodynia in rats. We further revealed that the transient receptor potential ankyrin subtype 1 protein (TRPA1) protein level is upregulated and the TRPA1 activator allyl isothiocyanate induced larger ion currents in the dorsal root ganglion neurons from the docetaxel treated rats...
March 31, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28370169/osteoclast-differentiation-gene-expression-profiling-reveals-chemokine-ccl4-mediates-rankl-induced-osteoclast-migration-and-invasion-via-pi3k-pathway
#2
Wenhua Xuan, Xiaoke Feng, Chen Qian, Liuying Peng, Yumeng Shi, Lingxiao Xu, Fang Wang, Wenfeng Tan
The migration of osteoclasts (OCs) from circulation and bone marrow into bone surface plays a critical role in the pathogenesis of some bone resorptive diseases, such as rheumatoid arthritis and osteoporosis. To date, how the migration of OCs remains unclear. We investigated gene expression profiling in osteoclastic differentiation of bone marrow-derived macrophages (BMMs) into OCs by microarray analysis. We identified 387 genes overexpressed in osteoclastic differentiation of BMMs. Among them, chemokine CCL4 showed a robust up-regulation signal...
March 30, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28370438/differential-activities-of-peroxisomes-along-the-mouse-intestinal-epithelium
#3
Petruta L Morvay, Myriam Baes, Paul P Van Veldhoven
The presence of peroxisomes in mammalian intestine has been revealed formerly by catalase staining combined with electron microscopy. Despite the central role of intestine in lipid uptake and the established importance of peroxisomes in different lipid-related pathways, few data are available on the physiological role of peroxisomes in intestinal metabolism, more specifically, α-, β-oxidation, and etherlipid synthesis. Hence, the peroxisomal compartment was analyzed in more detail in mouse intestine. On the basis of immunohistochemistry, the organelles are mainly confined to the epithelial cells...
March 29, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28303588/dietary-consumption-of-monosodium-l-glutamate-induces-adaptive-response-and-reduction-in-the-life-span-of-drosophila-melanogaster
#4
Amos O Abolaji, Charles O Olaiya, Oluwagbenga J Oluwadahunsi, Ebenezer O Farombi
Adaptive response is the ability of an organism to better counterattack stress-induced damage in response to a number of different cytotoxic agents. Monosodium L-glutamate (MSG), the sodium salt of amino acid glutamate, is commonly used as a food additive. We investigated the effects of MSG on the life span and antioxidant response in Drosophila melanogaster (D. melanogaster). Both genders (1 to 3 days old) of flies were fed with diet containing MSG (0.1, 0.5, and 2.5-g/kg diet) for 5 days to assess selected antioxidant and oxidative stress markers, while flies for longevity were fed for lifetime...
March 16, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28256024/rip3-deficiency-exacerbates-inflammation-in-dextran-sodium-sulfate-induced-ulcerative-colitis-mice-model
#5
Yu-Lin Xu, Hai-Lin Tang, Shi-Ying Zhu, Hao-Ran Peng, Zhong-Tian Qi, Wen Wang
Ulcerative colitis (UC) is a chronic intestinal inflammatory disease. The receptor-interacting protein kinase 3 (RIP3) was reported to be involved in many inflammatory disease. However, the mechanism of RIP3 in the pathogenesis of UC is still unclear. To investigate the effects and possible mechanism of RIP3 in UC pathogenesis, RIP3-/- mice was used in dextran sulfate sodium (DSS)-induced colitis model. It was found that by DSS-induced colitis, RIP3-/- mice showed significantly enhanced colitis symptoms, including increased weight loss, colon shortening, and colonic mucosa damage and severity, but decreased production of interleukin 6 and interleukin 1β...
March 2, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28261837/mir-199a-5p-suppresses-tumorigenesis-by-targeting-clathrin-heavy-chain-in-hepatocellular-carcinoma
#6
Guo-Hao Huang, Hong Shan, Dan Li, Bin Zhou, Peng-Fei Pang
The deregulation of microRNA (miRNA) is frequently associated with a variety of cancers, including hepatocellular carcinoma (HCC). In this study, we investigated the expression and possible role of miR-199a-5p in HCC. The expression of miR-199a-5p was measured by quantitative RT-PCR in HCC. The effect of miR-199a-5p was evaluated by cell viability and colony formation assays in HCC cell lines and tumor cell growth assay in xenograft nude mice. Quantitative real time PCR results showed that miR-199a-5p was down-regulated in 77...
March 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28256001/expression-subcellular-localisation-and-possible-roles-of-dipeptidyl-peptidase-9-dpp9-in-murine-macrophages
#7
Emilija Zapletal, Barbara Cupic, Jelka Gabrilovac
Dipeptidyl peptidase 9 (DPP9) is a peptidase of the DPPIV gene family, and its role in immune responses has been reported. In this study, we compared the messenger RNA expression profile of DPP9 to that of the related DPP8 and DPPIV in murine haematopoietic and lymphatic tissues. A similar order of expression levels was observed for all 3 peptidases: peritoneal macrophages < bone marrow < spleen ≤ lymph nodes. Also, we examined the subcellular localisation of DPP9 and its possible role(s) in J774 cell line of macrophage origin...
March 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28233339/paracrine-effect-of-cxcr4-overexpressing-mesenchymal-stem-cells-on-ischemic-heart-injury
#8
Shi-Zheng Wu, Ying-Lan Li, Wei Huang, Wen-Feng Cai, Jialiang Liang, Christian Paul, Lin Jiang, Zhi-Chao Wu, Meifeng Xu, Ping Zhu, Yigang Wang
It has been reported that CXCR4-overexpressing mesenchymal stem cells (MSC(CX4) ) can repair heart tissue post myocardial infarction. This study aims to investigate the MSCCX4-derived paracrine cardio-protective signaling in the presence of myocardial infarction. Mesenchymal stem cells (MSCs) were divided into 3 groups: MSC only, MSC(CX4) , and CXCR4 gene-specific siRNA-transduced MSC. Mesenchymal stem cells were exposed to hypoxia, and then MSCs-conditioned culture medium was incubated with neonatal and adult cardiomyocytes, respectively...
March 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28217922/characterization-of-e-ntpdase-ec-3-6-1-5-activity-in-hepatic-lymphocytes-a-different-activity-profile-from-peripheral-lymphocytes
#9
Pedro H Doleski, Stephen A Adefegha, Fernanda L Cabral, Daniela B R Leal
The activity of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5) was characterized in hepatic lymphocytes (HL) of rats. For this purpose, a specific method for the isolation of lymphocytes from hepatic tissue was developed. Subsequently, E-NTPDase activity of rat HL was compared with that of rat peripheral lymphocytes. The HL showed high cell count and viability. Also, the characterization test revealed that the optimal E-NTPDase activities were attained at 37°C and pH 8.0 in the presence of Ca(2+) ...
March 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28211084/endothelial-cells-biophysical-biochemical-and-chromosomal-aberrancies-in-high-glucose-condition-within-the-diabetic-range
#10
Aysa Rezabakhsh, Elahe Nabat, Mina Yousefi, Soheila Montazersaheb, Omid Cheraghi, Amir Mehdizadeh, Farzaneh Fathi, Ali Akbar Movassaghpour, Nasrin Maleki-Dizaji, Reza Rahbarghazi, Alireza Garjani
To date, many studies have been conducted to find out the underlying mechanisms of hyperglycemia-induced complications in diabetes mellitus, attributed to the cellular pathologies of different cells-especially endothelial cells. However, there are still many ambiguities and unresolved issues to be clarified. Here, we investigated the alteration in biophysical and biochemical properties in human umbilical vein endothelial cells exposed to a high-glucose concentration (30mM), comparable to glucose content in type 2 diabetes mellitus, over a course of 120 hours...
March 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28150318/determine-exogenous-human-ddah2-gene-function-in-rabbit-bone-marrow-derived-endothelial-progenitor-cells-in-vitro
#11
Sara Shoeibi, Shabnam Mohammadi, Hamid Reza Sadeghnia, Elahe Mahdipour, Majid Ghayour-Mobarhan
The in vitro amplification of endothelial progenitor cells (EPCs) is an important method because of its role in gene transferring and regenerative medicine. In this study, we isolated rabbit bone marrow-derived EPCs to further manipulation and overexpression of dimethylarginine dimethylaminohydrolase (DDAH) in EPCs. Isolated EPCs were cultured, expanded in endothelial basal medium. Morphology of EPCs and expression levels of surface markers detected using immunocytochemistry staining and through the use of flow cytometery...
March 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28120341/tumor-necrosis-factor-suppresses-interleukin-10-in-peripheral-b-cells-via-upregulating-bcl2-like-protein-12-in-patients-with-inflammatory-bowel-disease
#12
Xiutian Guo, Mao-Gang Li, Shan-Shan Li, Feng-Hua Liu, Zhan-Ju Liu, Ping-Chang Yang
The pathogenesis of the immune regulation dysfunction is unclear. Bcl2-like protein 12 (Bcl2L12) has immune suppression function. This study tests a hypothesis that tumor necrosis factor (TNF) increases Bcl2L12 to suppress the expression of interleukin (IL) 10 in peripheral B cells of patients with inflammatory bowel disease (IBD). In this study, peripheral blood samples were collected from IBD patients and healthy controls. B cells were isolated from the blood samples. The expression of IL-10 and Bcl2L12 in B cells was analyzed by quantitative reverse transcription polymerase chain reaction and Western blotting...
March 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28083967/strain-uses-gap-junctions-to-reverse-stimulation-of-osteoblast-proliferation-by-osteocytes
#13
Rosemary F L Suswillo, Behzad Javaheri, Simon C F Rawlinson, Gary P Dowthwaite, Lance E Lanyon, Andrew A Pitsillides
Identifying mechanisms by which cells of the osteoblastic lineage communicate in vivo is complicated by the mineralised matrix that encases osteocytes, and thus, vital mechanoadaptive processes used to achieve load-bearing integrity remain unresolved. We have used the coculture of immunomagnetically purified osteocytes and primary osteoblasts from both embryonic chick long bone and calvariae to examine these mechanisms. We exploited the fact that purified osteocytes are postmitotic to examine both their effect on proliferation of primary osteoblasts and the role of gap junctions in such communication...
January 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28083966/small-animal-models-to-understand-pathogenesis-of-osteoarthritis-and-use-of-stem-cell-in-cartilage-regeneration
#14
REVIEW
Virginia Piombo
Osteoarthritis (OA) is one of the most common diseases, which affect the correct functionality of synovial joints and is characterized by articular cartilage degradation. Limitation in the treatment of OA is mostly due to the very limited regenerative characteristic of articular cartilage once is damaged. Small animal models are of particular importance for mechanistic analysis to understand the processes that affect cartilage degradation. Combination of joint injury techniques with the use of stem cells has been shown to be an important tool for understanding the processes of cartilage degradation and regeneration...
January 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28083965/mechanistic-insight-into-nuclear-receptor-mediated-regulation-of-bile-acid-metabolism-and-lipid-homeostasis-by-grape-seed-procyanidin-extract-gspe
#15
REVIEW
Laura E Downing, Daniel Edgar, Patricia A Ellison, Marie-Louise Ricketts
Dietary procyanidins have emerged as important bioactive components that regulate various metabolic pathways to maintain homeostasis. Grape seed procyanidin extract (GSPE), in particular, has demonstrated regulatory effects on bile acid and lipid metabolism in vivo. While numerous studies in rodent models have shown the potent hypolipidemic action of grape seed extracts, human studies have shown inconsistent results. This review will focus on the molecular mechanisms underlying the hypolipidemic actions of GSPE identified to date, specifically highlighting the effects exerted via nuclear receptors...
January 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28083964/stimulation-of-neurotrophic-factors-and-inhibition-of-proinflammatory-cytokines-by-exogenous-application-of-triiodothyronine-in-the-rat-model-of-ischemic-stroke
#16
Fatemeh Sabbaghziarani, Keywan Mortezaee, Mohammad Akbari, Iraj Ragerdi Kashani, Mansooreh Soleimani, Gholamreza Hassanzadeh, Adib Zendedel
There is a positive relation between decreases of triiodothyronine (T3) amounts and severity of stroke. The aim of this study was to evaluate the effect of exogenous T3 application on levels of neurogenesis markers in the subventricular zone. Cerebral ischemia was induced by middle cerebral artery occlusion in male Wistar rats. There were 4 experimental groups: sham, ischemic, vehicle, and treatment. Rats were injected with T3 (25 μg/kg, IV injection) at 24 hours after ischemia. Animals were sacrificed at day 7 after ischemia...
January 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28052355/mitochondrial-ryanodine-sensitive-ca-2-channels-of-rat-liver
#17
N I Kupynyak, O V Ikkert, S G Shlykov, L G Babich, V V Manko
To examine ryanodine-sensitive Ca(2+) channels in mitochondria of rat hepatocytes and their role in energy state of the cells via investigation of the ryanodine effect on mitochondrial membrane potential. Oxygen consumption was measured by polarography using the Clark electrode. The substrates of oxidation such as pyruvate (5mM), α-ketoglutarate (5mM), or succinate (5mM) were used. Oxidative phosphorylation was stimulated by the addition of adenosine diphosphate (200nM). Mitochondrial membrane potential was measured using a voltage-sensitive fluorescent probe tetramethylrhodamine-methyl-ester (0...
January 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28052347/apoptotic-effects-of-bovine-apo-lactoferrin-on-hela-tumor-cells
#18
Carla Luzi, Fabrizia Brisdelli, Roberto Iorio, Argante Bozzi, Veronica Carnicelli, Antonio Di Giulio, Anna Rita Lizzi
Lactoferrin (Lf), a cationic iron-binding glycoprotein of 80 kDa present in body secretions, is known as a compound with marked antimicrobial activity. In the present study, the apoptotic effect of iron-free bovine lactoferrin (apo-bLf) on human epithelial cancer (HeLa) cells was examined in association with reactive oxygen species and glutathione (GSH) levels. Apoptotic effect of iron-free bovine lactoferrin inhibited the growth of HeLa cells after 48 hours of treatment while the diferric-bLf was ineffective in the concentration range tested (from 1 to 12...
January 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/27935137/regulation-mechanism-of-pdk1-on-macrophage-metabolism-and-function
#19
REVIEW
Yueqin Yang, Weiwei Kong, Zhi Xia, Lin Xiao, Song Wang
PDK1 is a member of the atypical glandular cell kinases family that regulates the activities of most atypical glandular cell kinases during different development stages and treatment of cancers. PDK1 is also a critical glucose metabolism enzyme regulating glucolysis or glucose oxidase in cells, and more research is needed to further understand the underlying mechanism. The research of PDK1 presented by recent studies focuses much on cancer treatment and has helped researchers gain much insight in this regard...
December 2016: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/27935136/a-possible-s-glutathionylation-of-specific-proteins-by-glyoxalase-ii-an-in-vitro-and-in-silico-study
#20
Luisa Ercolani, Andrea Scirè, Roberta Galeazzi, Luca Massaccesi, Laura Cianfruglia, Adolfo Amici, Francesco Piva, Lorena Urbanelli, Carla Emiliani, Giovanni Principato, Tatiana Armeni
Glyoxalase II, the second of 2 enzymes in the glyoxalase system, is a hydroxyacylglutathione hydrolase that catalyses the hydrolysis of S-d-lactoylglutathione to form d-lactic acid and glutathione, which is released from the active site. The tripeptide glutathione is the major sulfhydryl antioxidant and has been shown to control several functions, including S-glutathionylation of proteins. S-Glutathionylation is a way for the cells to store reduced glutathione during oxidative stress, or to protect protein thiol groups from irreversible oxidation, and few enzymes involved in protein S-glutathionylation have been found to date...
December 2016: Cell Biochemistry and Function
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