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Cell Biochemistry and Function

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https://www.readbyqxmd.com/read/29143345/lin28a-enhances-in-vitro-osteoblastic-differentiation-of-human-periosteum-derived-cells
#1
Jin-Ho Park, Bong-Wook Park, Young-Hoon Kang, Sung-Hoon Byun, Sun-Chul Hwang, Deok Ryong Kim, Dong Kyun Woo, June-Ho Byun
Despite a capacity for proliferation and an ability to differentiate into multiple cell types, in long-term culture and with ageing, stem cells show a reduction in growth, display a decrease in differentiation potential, and enter senescence without evidence of transformation. The Lin28a gene encodes an RNA-binding protein that plays a role in regulating stem cell activity, including self-renewal and differentiation propensity. However, the effect of the Lin28a gene on cultured human osteoprecursor cells is poorly understood...
November 16, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29143344/down-regulation-of-p16-and-mgmt-promotes-the-anti-proliferative-and-pro-apoptotic-effects-of-5-aza-dc-and-radiation-on-cervical-cancer-cells
#2
Guan-di Chen, De-Ying Qian, Zhi-Gang Li, Ge-Ying Fan, Ke-Li You, Yi-Long Wu
Cervical cancer is one of the most common malignancies of the female reproductive system. Therefore, it is critical to investigate the molecular mechanisms involved in the development and progression of cervical cancer. In this study, we stimulated cervical cancer cells with 5-aza-2'-deoxycytidine (5-Aza-dC) and found that this treatment inhibited cell proliferation and induced apoptosis; additionally, methylation of p16 and O-6-methylguanine-DNA methyltransferase (MGMT) was reversed, although their expression was suppressed...
November 15, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29063619/effect-of-an-acute-moderate-exercise-session-on-metabolic-and-inflammatory-profile-of-ppar-%C3%AE-knockout-mice
#3
Loreana S Silveira, Gustavo D Pimentel, Camila O Souza, Luana A Biondo, Alexandre Abílio S Teixeira, Edson A Lima, Helena A P Batatinha, José C Rosa Neto, Fábio S Lira
Peroxisome proliferator-activated receptors (PPARs) play a major role in metabolism and inflammatory control. Exercise can modulate PPAR expression in skeletal muscle, adipose tissue, and macrophages. Little is known about the effects of PPAR-α in metabolic profile and cytokine secretion after acute exercise in macrophages. In this context, the aim of this study was to understand the influence of PPAR-α on exercise-mediated immune metabolic parameters in peritoneal macrophages. Mice C57BL/6 (WT) and PPAR-α knockout (KO) were examined in non-exercising control (n = 4) or 24 hours after acute moderate exercise (n = 8)...
October 23, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29052243/wnt-%C3%AE-catenin-signalling-pathway-mediated-aberrant-hippocampal-neurogenesis-in-kainic-acid-induced-epilepsy
#4
Zhengyi Qu, Fang Su, Xueting Qi, Jianbo Sun, Hongcai Wang, Zhenkui Qiao, Hong Zhao, Yulan Zhu
Temporal lobe epilepsy is a chronic disorder of nerve system, mainly characterized by hippocampal sclerosis with massive neuronal loss and severe gliosis. Aberrant neurogenesis has been shown in the epileptogenesis process of temporal lobe epilepsy. However, the molecular mechanisms underlying aberrant neurogenesis remain unclear. The roles of Wnt signalling cascade have been well established in neurogenesis during multiple aspects. Here, we used kainic acid-induced rat epilepsy model to investigate whether Wnt/β-catenin signalling pathway is involved in the aberrant neurogenesis in temporal lobe epilepsy...
October 19, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29027248/ursodeoxycholic-acid-protects-cardiomyocytes-against-cobalt-chloride-induced-hypoxia-by-regulating-transcriptional-mediator-of-cells-stress-hypoxia-inducible-factor-1%C3%AE-and-p53-protein
#5
Anis Syamimi Mohamed, Noorul Izzati Hanafi, Siti Hamimah Sheikh Abdul Kadir, Julina Md Noor, Narimah Abdul Hamid Hasani, Sharaniza Ab Rahim, Rosfaiizah Siran
In hepatocytes, ursodeoxycholic acid (UDCA) activates cell signalling pathways such as p53, intracellular calcium ([Ca(2+) ]i ), and sphingosine-1-phosphate (S1P)-receptor via Gαi -coupled-receptor. Recently, UDCA has been shown to protect the heart against hypoxia-reoxygenation injury. However, it is not clear whether UDCA cardioprotection against hypoxia acts through a transcriptional mediator of cells stress, HIF-1α and p53. Therefore, in here, we aimed to investigate whether UDCA could protect cardiomyocytes (CMs) against hypoxia by regulating expression of HIF-1α, p53, [Ca(2+) ]i , and S1P-Gαi -coupled-receptor...
October 12, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29023897/atf1-and-ras-in-exosomes-are-potential-clinical-diagnostic-markers-for-cervical-cancer
#6
Yanhua Shi, Wei Wang, Baozhi Yang, Hongge Tian
Cervical cancer is one of the most common cancers among women worldwide. It is highly lethal yet can be treated when found in early stage. Thus, early detection is of significant important for early diagnosis of cervical cancer. Exosomes have been used as biomarkers in clinical diagnosis. It is unknown that whether blood exosomes associated with cervical cancer can be detected and if these exosomes can accurately represent the developmental stage of cervical cancer. Mouse models were made out of a relapsed cervical cancer patient's tumour sample for original and recurrent cervical cancer, and gene analysis in both tumours and exosomes in these mouse models were performed...
October 10, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29023799/mir-214-activates-tp53-but-suppresses-the-expression-of-rela-ctnnb1-and-stat3-in-human-cervical-and-colorectal-cancer-cells
#7
Karthik Subramanian Chandrasekaran, Anusha Sathyanarayanan, Devarajan Karunagaran
High Mobility Group AT-hook 1 (HMGA1) was identified as a target of miR-214 in human cervical and colorectal cancers (CaCx and CRC) in a previous study. While the expression of miR-214 remains suppressed, HMGA1 behaves as a potent oncogene and plays crucial roles in several aberrant signalling pathways by interacting with intermediates like RELA, CTNNB1, STAT3, and TP53 in CaCx and CRC. Hypothetically, miR-214 should be able to regulate the stabilization of some of these intermediates through the regulation of HMGA1...
October 10, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28990218/a-polysaccharide-from-dictyophora-indusiata-inhibits-the-immunosuppressive-function-of-cancer-associated-fibroblasts
#8
Shuyu Han, Chaoxiong Ma, Minghua Hu, Yuanyuan Wang, Fangli Ma, Ning Tao, Zhihai Qin
Reversing the function of cancer-associated fibroblasts (CAFs) may improve the efficacy of cancer therapy. Here, we isolated a novel polysaccharide from Dictyophora indusiata (ZSP4) and examined its effects on the function of prostate CAFs. The supernatant of prostate CAFs can stimulate the proliferation of immune cells and inhibit the growth of CD4+/CD8+ T cells. However, after ZSP4 stimulation, the functions of prostate CAFs were inhibited. The mechanism experiment shows that ZSP4 can stimulate prostate CAFs by down-regulating the expression of α-smooth muscle actin...
October 8, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28975647/effect-of-metformin-and-celecoxib-on-cytotoxicity-and-release-of-gdf-15-from-human-mesenchymal-stem-cells-in-high-glucose-condition
#9
Elaheh Zafarvahedian, Azam Roohi, Mohammad Reza Sepand, Seyed Nasser Ostad, Mohammad Hossein Ghahremani
Mesenchymal stem cells (MSCs) have therapeutic potential for treatment of diabetes. However, in vitro behavior of MSCs in high glucose condition as well as presence of glucose lowering agents is not fully understood. Because MSCs have an important role in tissue repair, we examined the effects of metformin and celecoxib on viability of MSCs in different glucose conditions. MSCs, from umbilical cord blood, were cultured in normoglycemic (glucose 5.5 mM), midglycemic (glucose 10 mM), and hyperglycemic (glucose 25 mM) conditions, and the cell viability was evaluated by MTT assay...
October 4, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28967682/differential-expression-of-micrornas-in-tm3-leydig-cells-of-mice-treated-with-brain-derived-neurotrophic-factor
#10
Shan Gao, Chunjin Li, Ying Xu, Shuxiong Chen, Yun Zhao, Lu Chen, Yanwen Jiang, Zhuo Liu, Rong Fan, Liting Sun, Fengge Wang, Xiaoling Zhu, Jing Zhang, Xu Zhou
Brain-derived neurotrophic factor (BDNF) is a neurotrophin that can promote the development and proliferation of neurons. BDNF has been found to be involved in male reproduction. Leydig cells in testicular interstitial tissues can secrete testosterone in a luteinizing hormone-dependent manner. We showed that BDNF and its receptor TrkB were expressed in mice TM3 Leydig cells in the present study. Furthermore, BDNF can promote proliferation of mouse TM3 Leydig cells in vitro. Results of microRNA (miRNA) deep sequencing showed that BDNF can alter the expression profile of miRNAs in TM3 Leydig cells...
October 2, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28961318/hoxa9-is-critical-in-the-proliferation-differentiation-and-malignancy-of-leukaemia-cells-both-in-vitro-and-in-vivo
#11
Shibing Chen, Juan Yu, Xin Lv, Lijuan Zhang
Progress in the understanding of the molecular mechanism for acute myeloid leukaemia is of great significance to generate new potential targets for treatment. Recent studies showed that HOXA9, a homeodomain-containing transcription factor, is commonly deregulated in acute leukaemia. In this study, we elucidated the direct correlation between HoxA9 expression and progression of leukaemia using 2 different types of leukaemia cells HL-60 and MOLT-3. The HoxA9 expression level was decreased in leukaemia cells with the treatment of all-trans retinoic acid or arsenic trioxide (As2 O3 )...
September 29, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28940415/mesenchymal-stromal-cells-ameliorate-acute-allergic-rhinitis-in-rats
#12
Chunlei Li, Yanxia Fu, Yinyin Wang, Yanhua Kong, Mengdi Li, Danhui Ma, Wanli Zhai, Hao Wang, Yuting Lin, Sihan Liu, Fangli Ren, Jun Li, Yi Wang
Mesenchymal stromal cells (MSCs) have been extensively investigated as a potential antiinflammatory treatment in many inflammatory-related diseases; however, it remains unclear whether MSCs could be used to treat acute allergic rhinitis. A rat model of allergic rhinitis was treated with MSCs. The effect of MSCs on the inflammation of allergic rhinitis was evaluated by sneezing, nose rubbing, the pathology of the nasal mucosa, and the expression of interleukin 4, tumour necrosis factor alpha, and immunoglobulin E in the serum of rats...
September 20, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28906008/role-of-macrophage-migration-inhibitory-factor-mif-in-the-effects-of-oxidative-stress-on-human-retinal-pigment-epithelial-cells
#13
Ji-Ae Ko, Yasuyuki Sotani, Diah Gemala Ibrahim, Yoshiaki Kiuchi
Proliferative vitreoretinopathy (PVR) is the major cause of treatment failure in individuals who undergo surgery for retinal detachment. The epithelial-mesenchymal transition (EMT) in retinal pigment epithelium (RPE) cells contributes to the pathogenesis of PVR. Oxidative stress is thought to play a role in the progression of retinal diseases including PVR. We have now examined the effects of oxidative stress on the EMT and related processes in the human RPE cell line. We found that H2 O2 induced the contraction of RPE cells in a three-dimensional collagen gel...
September 14, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28895148/critical-roles-of-hmagea2-in-induced-pluripotent-stem-cell-pluripotency-proliferation-and-differentiation
#14
Song Park, Yonghun Sung, Jain Jeong, Minjee Choi, Jinhee Lee, Wookbong Kwon, Soyoung Jang, Si Jun Park, Jae Young Kim, Sung Hyun Kim, Duhak Yoon, Zae Young Ryoo, Myoung Ok Kim
Induced pluripotent stem (iPS) cells are important for clinical application and stem cell research. Although human melanoma-associated antigen A2 (hMAGEA2) expression is known to affect differentiation in embryonic stem cells, its specific role in iPS cells remains unclear. To evaluate the function of hMAGEA2 and its characteristics in iPS cells, we produced hMAGEA2-overexpressing iPS cells from hMAGEA2-overexpressing transgenic mice. Although the iPS cells with overexpressed hMAGEA2 did not differ in morphology, their pluripotency, and self-renewal related genes (Nanog, Oct3/4, Sox2, and Stat3), expression level was significantly upregulated...
September 11, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28871607/characterization-of-ectonucleoside-triphosphate-diphosphohydrolase-e-ntpdase-ec-3-6-1-5-activity-in-mouse-peritoneal-cavity-cells
#15
Dhébora Albuquerque Dias, Bruna de Barros Penteado, Lucas Derbocio Dos Santos, Pedro Mendes Dos Santos, Carla Cardozo Pinto Arruda, Maria Rosa Chitolina Schetinger, Daniela Bitencourt Rosa Leal, Jeandre Augusto Dos Santos Jaques
This study aimed to characterize the activity of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5) in peritoneal cavity cells from BALB/c mice. E-NTPDase was activated in the presence of both calcium (1.5mM) and magnesium (1.5mM) ions. However, the activity was higher in the presence of Ca(2+) . A pH of 8.5 and temperature of 37°C were the optimum conditions for catalysis. The apparent Km values were 0.51mM and 0.66mM for the hydrolysis of adenosine triphosphate (ATP) and adenosine diphosphate (ADP), respectively...
September 4, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28871597/activation-of-the-mitochondrial-apoptotic-pathway-contributes-to-methotrexate-induced-small-intestinal-injury-in-rats
#16
Kasthuri Natarajan, Premila Abraham, Rekha Kota
The efficacy of methotrexate (MTX), a commonly used chemotherapeutic drug, is limited by intestinal injury. As the mechanism of MTX-induced small intestinal injury is not clear, there is no definitive treatment for MTX-induced gastrointestinal injury. The present study investigates the role of mitochondrial apoptotic pathway in MTX-induced small intestinal injury and examines whether aminoguanidine is effective in preventing the damage. Eight Wistar rats were administered 3 consecutive i.p. injections of 7 mg/kg body wt...
September 4, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28849601/effect-of-orthodontic-force-on-the-expression-of-pi3k-akt-and-p70s6-k-in-the-human-periodontal-ligament-during-orthodontic-loading
#17
Yunhe Xu, Jiayuan Shen, Fenik Kaml Muhammed, Bowen Zheng, Yuejiao Zhang, Yi Liu
The mammalian target of rapamycin (mTOR) is an atypical serine/threonine protein kinases involved in the regulation of cell growth, proliferation, and differentiation through the PI3K/Akt/mTOR/P70S6 K signalling pathway. P70S6 K as a downstream molecule of mTOR is activated by phosphorylation and subsequently promotes the synthesis of ribosomal and translational proteins. In this study, we investigated the role of PI3K, Akt, and P70S6 K in human periodontal tissue remodelling during orthodontic loading. The prepared tissue specimens taken from 4 extracted premolars were processed for immunolabelling...
August 28, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28849597/protection-of-bone-marrow-mononuclear-and-cd34-cells-by-enclosing-within-the-biochemical-compound-solution-during-and-after-transplantation
#18
Durdi Qujeq, Zeinab Abedian
We have chosen collagen, chitosan acetate, hyaluronic acid, and propolis as model biochemical compound solution to determine the influence of cell carrier mechanics on cell viability and functionality during and after transplantation. Suspending of bone marrow (BM), mononuclear (MN), and CD34+ cells into a biochemical compounds solution is an attractive tool to achieve to protect and ensure reproducible deliver. Hyperglycemic rats were randomly divided into 2 groups: to receive no cell treatment or approximately 1 × 10(5) of BM, MN, and CD34+ cells within the PBS or biochemical compound solution...
August 28, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28849590/lrf-inhibits-p53-expression-in-colon-cancer-cells-via-modulating-dap5-activity
#19
Min Zhu, Peng Wang, Fan Feng, Ming-Yang Li
The p53 protein plays a critical role in suppression of tumour growth; its regulation is not fully understood. Leukaemia/lymphoma-related factor (LRF) promotes tumour cell growth. This study tests a hypothesis that LRF inhibits p53 expression in colon cancer cells. In this study, human colon cancer cell lines, LIM1215 and HCT116 cells, were used. The expression of LRF and p53 in the cells was analysed by quantitative reverse transcription polymerase chain reaction and Western blotting. We observed that the expression of protease-activated receptor 2 (PAR2) was detected in both LIM1215 and HCT116 human colon cancer cells...
August 28, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29082591/the-involvement-of-histone-methylation-in-osteoblastic-differentiation-of-human-periosteum-derived-cells-cultured-in-vitro-under-hypoxic-conditions
#20
Dae-Kwan Yoon, Ji-Sung Park, Gyu-Jin Rho, Hyeon-Jeong Lee, Iel-Yong Sung, Jang-Ho Son, Bong-Wook Park, Young-Hoon Kang, Sung-Hoon Byun, Sun-Chul Hwang, Dong Kyun Woo, Yeong-Cheol Cho, June-Ho Byun
Although oxygen concentrations affect the growth and function of mesenchymal stem cells (MSCs), the impact of hypoxia on osteoblastic differentiation is not understood. Likewise, the effect of hypoxia-induced epigenetic changes on osteoblastic differentiation of MSCs is unknown. The aim of this study was to examine the in vitro hypoxic response of human periosteum-derived cells (hPDCs). Hypoxia resulted in greater proliferation of hPDCs as compared with those cultured in normoxia. Further, hypoxic conditions yielded decreased expression of apoptosis- and senescence-associated genes by hPDCs...
October 2017: Cell Biochemistry and Function
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