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Avian Pathology: Journal of the W.V.P.A

Dieter Liebhart, Michael Hess
No abstract text is available yet for this article.
December 15, 2017: Avian Pathology: Journal of the W.V.P.A
Heping Huangfu, Wenbo Xu, Hongkui Wang, Qing Dong, Hongwei Guo, Yanting Sun, Yunxia Li, Wenwen Gao, Wuqing Wang, Jia Zhang, Jingke Shi, Haochun Pan, Congcong Li, Linkang Wang
To better understand the prevalence of Gallibacterium anatis in different poultry species, a rapid and accurate method was developed to detect G. anatis using a TaqMan fluorescent quantitative polymerase chain reaction (qPCR). Specific primers and a TaqMan probe were designed based on the reference gtxA gene sequence. The qPCR standard curve showed a good linear relationship, and the method showed good reproducibility, sensitivity, and specificity, indicating its suitability for G. anatis identification and quantitative analysis...
December 15, 2017: Avian Pathology: Journal of the W.V.P.A
Lijuan Xu, Zijian Liu, Yang Li, Chao Yin, Yachen Hu, Xiaolei Xie, Qiuchun Li, Xinan Jiao
Salmonella enterica serovar Gallinarum biovar Pullorum (S. Pullorum) is the pathogen of pullorum disease, which leads to severe economic losses in many developing countries. Traditional methods to identify S. enterica have relied on biochemical reactions and serotyping, which are time-consuming with accurate identification if properly carried out. In this study, we developed a rapid polymerase chain reaction (PCR) method targeting the specific gene ipaJ to detect S. Pullorum. Among the 650 S. Pullorum strains isolated from 1962 to 2016 all over China, 644 strains were identified to harbor ipaJ gene in the plasmid pSPI12 accounting to a detection rate of 99...
December 12, 2017: Avian Pathology: Journal of the W.V.P.A
Ursula Höfle, Marco van de Bildt, Lonneke Leijten, Geert Van Amerongen, Josanne H Verhagen, Ron A M Fouchier, Albert Osterhaus, Thijs Kuiken
Black-headed gulls (Chroicocephalus ridibundus) are a suitable host species to study the epidemiology of low pathogenic avian influenza virus (LPAIV) infection in wild waterbirds because they are a common colony-breeding species in which LPAIV infection is detected frequently, limited mainly to the H13 and H16 subtypes. However, the sites of virus replication and associated lesions are poorly understood. Therefore, we performed virological and pathological analyses on tissues of black-headed gulls naturally infected with LPAIV...
November 27, 2017: Avian Pathology: Journal of the W.V.P.A
Rebecca Knab, Henning Petersen, Silke Rautenschlein, Arne Jung
Despite the importance of Bordetella avium (BA) as a respiratory pathogen of young turkeys, no infection model for the evaluation of BA-vaccine efficacy is available. The objective of this study was to evaluate the influence of route and dose of infection on the establishment of a BA-challenge model. In our first experiment, 28-day-old turkeys were either inoculated oculonasally with 10(5), 10(7) or 10(9) colony forming units (CFU) of BA per animal or exposed to BA by aerosol with 10(5)-10(8) CFU/m(3). The respiratory tract of all inoculated animals was BA-colonised, which was confirmed by choanal swabs and samples of trachea and lung, showing the highest prevalence in the aerosol inoculated group...
November 22, 2017: Avian Pathology: Journal of the W.V.P.A
Hafez M Hafez, Aly M Fadly, Yehia Mo Saif, Youssef Ibrahim
No abstract text is available yet for this article.
November 16, 2017: Avian Pathology: Journal of the W.V.P.A
Laura R Chen, M Mitsu Suyemoto, Albarra H Sarsour, H Alejandro Cordova, Edgar O Oviedo-Rondón, H John Barnes, Luke B Borst
Osteochondrosis (OCD) results from a disturbance of endochondral ossification in articular cartilage and is an important cause of lameness in several animal species, including chickens. OCD lesions in the free thoracic vertebra (FTV) of chickens are essential to the pathogenesis of pathogenic Enterococcus cecorum. The goal of this study was to determine the prevalence of OCD in the FTV among three modern broiler chicken crosses (strains A/A, A/B, and C/C) and Athens Canadian Random Bred (ACRB) chickens, which served as the control group...
November 15, 2017: Avian Pathology: Journal of the W.V.P.A
Abdelfattah H Eladl, Hamed R Hamed, Reham A El-Shafei
This study was carried out on six laying hen farms, three mite-infested and three mite-free at Dakahlia and Damietta governorates in Egypt to demonstrate: (i) prevalence of different species of mites on laying hen farms; (ii) effects of mite infestation on chicken health and production; (iii) efficacy of deltamethrin (DMT) on treatment of mite infestation and (iv) residues of DMT in eggs and meat. The results showed that 12 mite species were detected in the mite-infested farms, this is the first record in Egypt, and that Dermanyssus gallinae was the highest identified species from 295 (40...
November 9, 2017: Avian Pathology: Journal of the W.V.P.A
Dongchao Zhang, Yuqing Long, Meng Li, Jianfang Gong, Xiaohui Li, Jing Lin, Jiali Meng, Keke Gao, Ruili Zhao, Tianming Jin
Avian infectious bronchitis (IB) caused by the infectious bronchitis virus (IBV) and mycoplasmosis caused by Mycoplasma gallisepticum (MG) are two major respiratory diseases in chickens that have resulted in severe economic losses in the poultry industry. We constructed a recombinant adenovirus that simultaneously expresses the S1 spike glycoprotein of IBV and the TM-1 protein of MG (pBH-S1-TM-1-EGFP). For comparison, we constructed two recombinant adenoviruses (pBH-S1-EGFP and pBH-TM-1-EGFP) that express either the S1 spike glycoprotein or the TM-1 protein alone...
November 8, 2017: Avian Pathology: Journal of the W.V.P.A
Seyed Ahmad Madani, Fatemeh Arabkhazaeli, Elyas Shakeri, Sedigheh Nabian
Coccidian parasites especially Isospora are prevalent parasites in Passeriformes. Isosporan oocysts from common mynahs (Acridotheres tristis) are incompletely described. Detailed knowledge on biology, prevalence, pathogenesis and treatment of avian isosporiasis are scant. In this study isosporan oocysts isolated from common mynahs were morphologically and molecularly characterized. The medication efficiencies of diclazuril and sulfadiazine-trimethoprim in isosporiasis in naturally infected mynahs were evaluated...
November 8, 2017: Avian Pathology: Journal of the W.V.P.A
Najmeh Mosleh, Tahoora Shomali, Fahimeh Nematollahi, Zahra Ghahramani, Mohammad Saeid Ahrari Khafi, Fatemeh Namazi
This study evaluates the effect of different periods of chronic heat stress (CHS) on selected bone and serum parameters of broilers with or without vitamin C administration. Ninety 23 day old-chickens were randomly allocated into 7 groups: 1. control, 2. short term CHS (5 days), 3. short term CHS + vitamin C (12 g/100L drinking water of a 50% product) 4. medium term CHS (10 days), 5. medium term CHS + vitamin C, 6. long term CHS (20 days) and 7. long term CHS + vitamin C. In heat stressed groups the temperature was increased to 39±1°C for 8h/day...
November 6, 2017: Avian Pathology: Journal of the W.V.P.A
Anna Schachner, Miguel Matos, Beatrice Grafl, Michael Hess
The stand-alone pathogenicity of fowl adenoviruses (FAdVs) had long been disputed, given the ubiquity of the viruses versus sporadic outbreaks, and variation between experimental studies. However, a globally emerging trend of FAdV-associated diseases has marked the past two decades, with hepatitis-hydropericardium syndrome mainly in Asia besides Arabian and Latin American countries, and geographically more disseminated outbreaks of inclusion body hepatitis. Finally, the appearance of FAdV-induced gizzard erosion (AGE) in Asia and Europe completed the range of diseases...
October 25, 2017: Avian Pathology: Journal of the W.V.P.A
Vivek Pande, Andrea R McWhorter, Kapil K Chousalkar
This study evaluated the antibacterial activity of commercially available organic acid water additives against Salmonella enterica isolates and examined the susceptibility of Salmonella Typhimurium biofilms to these products. Three commercial organic acid products (A, B, and C) were evaluated for minimum inhibitory and bactericidal concentrations against isolates of S. enterica serovars. Three and five day old S. Typhimurium biofilms were formed at 22 ± 2°C using MBEC™ assay system and exposed for 30 and 90 min at 0...
October 23, 2017: Avian Pathology: Journal of the W.V.P.A
Sebastien Mathieu Soubies, Céline Courtillon, Mouna Abed, Michel Amelot, Alassane Keita, Andrew Broadbent, Sonja Härtle, Bernd Kaspers, Nicolas Eterradossi
Infectious bursal disease virus (IBDV) is a Birnaviridae family member of economic importance for poultry. This virus infects and destroys developing B lymphocytes in the cloacal bursa, resulting in a potentially fatal or immunosuppressive disease in chickens. Naturally-occurring viruses and many vaccine strains are not able to grow in in vitro systems without prior adaptation, which process often affects viral properties such as virulence. Primary bursal cells, which are the main target cells of lymphotropic IBDV in vivo, may represent an attractive system for the study of IBDV...
October 17, 2017: Avian Pathology: Journal of the W.V.P.A
Miguel A De la Rosa-Ramos, Karina Muñoz-Solís, Mario Palma-Zepeda, Adriana C Gutierrez-Castillo, Edgar O López Villegas, Fernando M Guerra-Infante, Graciela Castro-Escarpulli
Ornithobacterium rhinotracheale (O.rhinotracheale), is a bacterium that causes respiratory disease in birds and it has been isolated in countries with a large poultry production, including Mexico. The pathogenicity mechanisms of this bacterium have not been completely elucidated yet. The capacity of the bacterium to adhere to epithelial cells of chicken in vitro has been evidenced, and since this bacterium has been isolated from the lungs and air sacs of several avian species, the aim of this study was to determine if this bacterium can adhere to chicken lung cells...
October 10, 2017: Avian Pathology: Journal of the W.V.P.A
Quanxi Wang, Mengxi Liu, Lihui Xu, Yijian Wu, Yifan Huang
The aim of this work was to clarify the molecular mechanism underlying the fatty degeneration of livers infected with Muscovy duck reovirus (MDRV), which produces obvious white necrotic foci in the liver. Transcriptome data for MDRV-infected Muscovy duck livers and control livers were sequenced, assembled, and annotated with Illumina(®) HiSeq 2000. The differentially expressed genes were screened and their functions were analysed. We also determined and confirmed the molecular mechanism of the hepatic fat metabolism disorder caused by MDRV infection...
October 10, 2017: Avian Pathology: Journal of the W.V.P.A
Seiche C Genger, Keijiro Mizukami, Michael P Martin, Jeffrey R Applegate, H John Barnes, Urs Giger
Clinicopathological diagnosis of mucopolysaccharidosis type IIIB (MPS IIIB; Sanfilippo syndrome B), an inherited autosomal recessive lysosomal storage disease, as a cause of losses in a commercial emu flock and screening breeders using a mutation-specific DNA test are described. Between 2012 and 2015, ∼5-10 juvenile emus from a few weeks to several months of age developed progressive neurological signs and died while others in the flock remained healthy. Necropsy of two affected siblings revealed multiple sites of haemorrhage, cytoplasmic periodic acid-Schiff and Luxol fast blue-positive inclusions in neurons, and aggregates of foamy macrophages in visceral organs...
October 10, 2017: Avian Pathology: Journal of the W.V.P.A
J J de Wit, C Cazaban, R Dijkman, G Ramon, Y Gardin
To gather recent data regarding the infectious bronchitis (IB) and infectious bursal disease (IBD) situation in Europe, a large-scale field epidemiological survey using diagnostic samples has been implemented in 2013 for about six months in several European countries: France, Germany, Greece, Italy, the Netherlands, Poland, Portugal, the Republic of Ireland, Spain and the United Kingdom. In 234 flocks that were sampled, strains from 10 different IBV genotypes were detected: the 793B genotype was detected most frequently, followed by QX, Massachusetts (Mass) and the Xindadi-like strains...
October 3, 2017: Avian Pathology: Journal of the W.V.P.A
Franziska Feichtner, Anna Schachner, Evelyn Berger, Michael Hess
Conventional serological methods for detection and differentiation of antibodies against fowl aviadenoviruses (FAdVs) are laborious and time-consuming, therefore ELISAs based upon recombinant proteins were developed in the present study to overcome this limitation for clinically relevant serotypes FAdV-1 and FAdV-4. In order to develop serotype-specific ELISAs, the two distinct fibers, fiber-1 (fib-1) and fiber-2 (fib-2), characteristically present only in FAdV-1 and FAdV-4, were applied separately as coating antigens...
September 25, 2017: Avian Pathology: Journal of the W.V.P.A
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No abstract text is available yet for this article.
December 2017: Avian Pathology: Journal of the W.V.P.A
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