journal
MENU ▼
Read by QxMD icon Read
search

EMBO Journal

journal
https://www.readbyqxmd.com/read/28536151/is-magnetogenetics-the-new-optogenetics
#1
Simon Nimpf, David A Keays
No abstract text is available yet for this article.
May 23, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28536150/getting-stress-out-of-stressed-out-stress-granules
#2
Pratibha Siwach, Daniel Kaganovich
No abstract text is available yet for this article.
May 23, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28533231/nix-ing-mitochondria-from-development-to-pathology
#3
Aleksandra Deczkowska, Michal Schwartz
No abstract text is available yet for this article.
May 22, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28533230/deletion-of-pikfyve-alters-alveolar-macrophage-populations-and-exacerbates-allergic-inflammation-in-mice
#4
Takumi Kawasaki, Kosuke Ito, Haruhiko Miyata, Shizuo Akira, Taro Kawai
Alveolar macrophages (AMs) are specialized tissue-resident macrophages that orchestrate the immune responses to inhaled pathogens and maintain organ homeostasis of the lung. Dysregulation of AMs is associated with allergic inflammation and asthma. Here, we examined the role of a phosphoinositide kinase PIKfyve in AM development and function. Mice with conditionally deleted PIKfyve in macrophages have altered AM populations. PIKfyve deficiency results in a loss of AKT activation in response to GM-CSF, a cytokine critical for AM development...
May 22, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28533229/intrinsic-regulation-of-enteroendocrine-fate-by-numb
#5
Jérémy Sallé, Louis Gervais, Benjamin Boumard, Marine Stefanutti, Katarzyna Siudeja, Allison J Bardin
How terminal cell fates are specified in dynamically renewing adult tissues is not well understood. Here we explore terminal cell fate establishment during homeostasis using the enteroendocrine cells (EEs) of the adult Drosophila midgut as a paradigm. Our data argue against the existence of local feedback signals, and we identify Numb as an intrinsic regulator of EE fate. Our data further indicate that Numb, with alpha-adaptin, acts upstream or in parallel of known regulators of EE fate to limit Notch signaling, thereby facilitating EE fate acquisition...
May 22, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28515121/ampk%C3%AE-1-ldh-pathway-regulates-muscle-stem-cell-self-renewal-by-controlling-metabolic-homeostasis
#6
Marine Theret, Linda Gsaier, Bethany Schaffer, Gaëtan Juban, Sabrina Ben Larbi, Michèle Weiss-Gayet, Laurent Bultot, Collodet Caterina, Marc Foretz, Dominique Desplanches, Pascual Sanz, Zizhao Zang, Lin Yang, Guillaume Vial, Benoit Viollet, Kei Sakamoto, Anne Brunet, Bénédicte Chazaud, Rémi Mounier
Control of stem cell fate to either enter terminal differentiation versus returning to quiescence (self-renewal) is crucial for tissue repair. Here, we showed that AMP-activated protein kinase (AMPK), the master metabolic regulator of the cell, controls muscle stem cell (MuSC) self-renewal. AMPKα1(-/-) MuSCs displayed a high self-renewal rate, which impairs muscle regeneration. AMPKα1(-/-) MuSCs showed a Warburg-like switch of their metabolism to higher glycolysis. We identified lactate dehydrogenase (LDH) as a new functional target of AMPKα1...
May 17, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28507225/phosphorylation-of-pkp1-by-ripk4-regulates-epidermal-differentiation-and-skin-tumorigenesis
#7
Philbert Lee, Shangwen Jiang, Yuanyuan Li, Jiping Yue, Xuewen Gou, Shao-Yu Chen, Yingming Zhao, Markus Schober, Minjia Tan, Xiaoyang Wu
Tissue homeostasis of skin is sustained by epidermal progenitor cells localized within the basal layer of the skin epithelium. Post-translational modification of the proteome, such as protein phosphorylation, plays a fundamental role in the regulation of stemness and differentiation of somatic stem cells. However, it remains unclear how phosphoproteomic changes occur and contribute to epidermal differentiation. In this study, we survey the epidermal cell differentiation in a systematic manner by combining quantitative phosphoproteomics with mammalian kinome cDNA library screen...
May 15, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28507224/c-myc-mrna-tail-tale-about-glutamine-control-of-transcription
#8
Chi V Dang
Dejure et al (2017) demonstrates an intriguing link between glutamine, c-MYC protein levels, and c-MYC-dependent transcription. Glutamine-dependent c-MYC protein level, which is sensed through the c-MYC mRNA 3'-UTR, determines global transcriptional response to glutamine deprivation in HCT116 colon cancer cells. These findings add another layer of complexity to c-MYC's role as a nexus in metabolic regulation.
May 15, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28495680/dna-binding-determinants-and-cellular-thresholds-for-human-telomerase-repeat-addition-processivity
#9
Robert Alexander Wu, Jane Tam, Kathleen Collins
The reverse transcriptase telomerase adds telomeric repeats to chromosome ends. Purified human telomerase catalyzes processive repeat synthesis, which could restore the full ~100 nucleotides of (T2AG3)n lost from replicated chromosome ends as a single elongation event. Processivity inhibition is proposed to be a basis of human disease, but the impacts of different levels of processivity on telomere maintenance have not been examined. Here, we delineate side chains in the telomerase active-site cavity important for repeat addition processivity, determine how they contribute to duplex and single-stranded DNA handling, and test the cellular consequences of partial or complete loss of repeat addition processivity for telomere maintenance...
May 11, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28495679/autophagosome-formation-is-initiated-at-phosphatidylinositol-synthase-enriched-er-subdomains
#10
Taki Nishimura, Norito Tamura, Nozomu Kono, Yuta Shimanaka, Hiroyuki Arai, Hayashi Yamamoto, Noboru Mizushima
The autophagosome, a double-membrane structure mediating degradation of cytoplasmic materials by macroautophagy, is formed in close proximity to the endoplasmic reticulum (ER). However, how the ER membrane is involved in autophagy initiation and to which membrane structures the autophagy-initiation complex is localized have not been fully characterized. Here, we were able to biochemically analyze autophagic intermediate membranes and show that the autophagy-initiation complex containing ULK and FIP200 first associates with the ER membrane...
May 11, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28495678/sphingolipid-metabolic-flow-controls-phosphoinositide-turnover-at-the-trans-golgi-network
#11
Serena Capasso, Lucia Sticco, Riccardo Rizzo, Marinella Pirozzi, Domenico Russo, Nina A Dathan, Felix Campelo, Josse van Galen, Maarit Hölttä-Vuori, Gabriele Turacchio, Angelika Hausser, Vivek Malhotra, Isabelle Riezman, Howard Riezman, Elina Ikonen, Chiara Luberto, Seetharaman Parashuraman, Alberto Luini, Giovanni D'Angelo
Sphingolipids are membrane lipids globally required for eukaryotic life. The sphingolipid content varies among endomembranes with pre- and post-Golgi compartments being poor and rich in sphingolipids, respectively. Due to this different sphingolipid content, pre- and post-Golgi membranes serve different cellular functions. The basis for maintaining distinct subcellular sphingolipid levels in the presence of membrane trafficking and metabolic fluxes is only partially understood. Here, we describe a homeostatic regulatory circuit that controls sphingolipid levels at the trans-Golgi network (TGN)...
May 10, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28487411/a-microrna-129-5p-rbfox-crosstalk-coordinates-homeostatic-downscaling-of-excitatory-synapses
#12
Marek Rajman, Franziska Metge, Roberto Fiore, Sharof Khudayberdiev, Ayla Aksoy-Aksel, Silvia Bicker, Cristina Ruedell Reschke, Rana Raoof, Gary P Brennan, Norman Delanty, Michael A Farrell, Donncha F O'Brien, Sebastian Bauer, Braxton Norwood, Morten T Veno, Marcus Krüger, Thomas Braun, Jørgen Kjems, Felix Rosenow, David C Henshall, Christoph Dieterich, Gerhard Schratt
Synaptic downscaling is a homeostatic mechanism that allows neurons to reduce firing rates during chronically elevated network activity. Although synaptic downscaling is important in neural circuit development and epilepsy, the underlying mechanisms are poorly described. We performed small RNA profiling in picrotoxin (PTX)-treated hippocampal neurons, a model of synaptic downscaling. Thereby, we identified eight microRNAs (miRNAs) that were increased in response to PTX, including miR-129-5p, whose inhibition blocked synaptic downscaling in vitro and reduced epileptic seizure severity in vivo Using transcriptome, proteome, and bioinformatic analysis, we identified the calcium pump Atp2b4 and doublecortin (Dcx) as miR-129-5p targets...
May 9, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28483816/a-pseudomonas-aeruginosa%C3%A2-tir-effector-mediates-immune-evasion-by-targeting%C3%A2-ubap1-and-tlr-adaptors
#13
Paul Rc Imbert, Arthur Louche, Jean-Baptiste Luizet, Teddy Grandjean, Sarah Bigot, Thomas E Wood, Stéphanie Gagné, Amandine Blanco, Lydia Wunderley, Laurent Terradot, Philip Woodman, Steve Garvis, Alain Filloux, Benoit Guery, Suzana P Salcedo
Bacterial pathogens often subvert the innate immune system to establish a successful infection. The direct inhibition of downstream components of innate immune pathways is particularly well documented but how bacteria interfere with receptor proximal events is far less well understood. Here, we describe a Toll/interleukin 1 receptor (TIR) domain-containing protein (PumA) of the multi-drug resistant Pseudomonas aeruginosa PA7 strain. We found that PumA is essential for virulence and inhibits NF-κB, a property transferable to non-PumA strain PA14, suggesting no additional factors are needed for PumA function...
May 8, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28483815/drosophila-dalmatian-combines-sororin-and-shugoshin-roles-in-establishment-and-protection-of-cohesion
#14
Takashi Yamada, Eri Tahara, Mai Kanke, Keiko Kuwata, Tomoko Nishiyama
Sister chromatid cohesion is crucial to ensure chromosome bi-orientation and equal chromosome segregation. Cohesin removal via mitotic kinases and Wapl has to be prevented in pericentromeric regions in order to protect cohesion until metaphase, but the mechanisms of mitotic cohesion protection remain elusive in Drosophila Here, we show that dalmatian (Dmt), an ortholog of the vertebrate cohesin-associated protein sororin, is required for protection of mitotic cohesion in flies. Dmt is essential for cohesion establishment during interphase and is enriched on pericentromeric heterochromatin...
May 8, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28483814/dalmatian-spotting-the-difference-in-cohesin-protectors
#15
Adele L Marston
The cohesin complex prevents separation of chromosomes following their duplication until the appropriate time during cell division. In vertebrates, establishment and maintenance of cohesin-dependent linkages depend on two distinct proteins, sororin and shugoshin. New findings published in The EMBO Journal show that in Drosophila, the function of both of these cohesin regulators is carried out by a single hybrid protein, Dalmatian.
May 8, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28483813/an-activated-q-snare-sm-protein-complex-as-a-possible-intermediate-in-snare-assembly
#16
Shrutee Jakhanwal, Chung-Tien Lee, Henning Urlaub, Reinhard Jahn
Assembly of the SNARE proteins syntaxin1, SNAP25, and synaptobrevin into a SNARE complex is essential for exocytosis in neurons. For efficient assembly, SNAREs interact with additional proteins but neither the nature of the intermediates nor the sequence of protein assembly is known. Here, we have characterized a ternary complex between syntaxin1, SNAP25, and the SM protein Munc18-1 as a possible acceptor complex for the R-SNARE synaptobrevin. The ternary complex binds synaptobrevin with fast kinetics, resulting in the rapid formation of a fully zippered SNARE complex to which Munc18-1 remains tethered by the N-terminal domain of syntaxin1...
May 8, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28473450/zika-virus-induces-massive-cytoplasmic-vacuolization-and-paraptosis-like-death-in-infected-cells
#17
Blandine Monel, Alex A Compton, Timothée Bruel, Sonia Amraoui, Julien Burlaud-Gaillard, Nicolas Roy, Florence Guivel-Benhassine, Françoise Porrot, Pierre Génin, Laurent Meertens, Laura Sinigaglia, Nolwenn Jouvenet, Robert Weil, Nicoletta Casartelli, Caroline Demangel, Etienne Simon-Lorière, Arnaud Moris, Philippe Roingeard, Ali Amara, Olivier Schwartz
The cytopathic effects of Zika virus (ZIKV) are poorly characterized. Innate immunity controls ZIKV infection and disease in most infected patients through mechanisms that remain to be understood. Here, we studied the morphological cellular changes induced by ZIKV and addressed the role of interferon-induced transmembrane proteins (IFITM), a family of broad-spectrum antiviral factors, during viral replication. We report that ZIKV induces massive vacuolization followed by "implosive" cell death in human epithelial cells, primary skin fibroblasts and astrocytes, a phenomenon which is exacerbated when IFITM3 levels are low...
May 4, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28468753/structure-of-the-bacillus-subtilis-hibernating-100s-ribosome-reveals-the-basis-for-70s-dimerization
#18
Bertrand Beckert, Maha Abdelshahid, Heinrich Schäfer, Wieland Steinchen, Stefan Arenz, Otto Berninghausen, Roland Beckmann, Gert Bange, Kürşad Turgay, Daniel N Wilson
Under stress conditions, such as nutrient deprivation, bacteria enter into a hibernation stage, which is characterized by the appearance of 100S ribosomal particles. In Escherichia coli, dimerization of 70S ribosomes into 100S requires the action of the ribosome modulation factor (RMF) and the hibernation-promoting factor (HPF). Most other bacteria lack RMF and instead contain a long form HPF (LHPF), which is necessary and sufficient for 100S formation. While some structural information exists as to how RMF and HPF mediate formation of E...
May 3, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28468752/usp4-inhibits-smad4-monoubiquitination-and-promotes-activin-and-bmp-signaling
#19
Fangfang Zhou, Feng Xie, Ke Jin, Zhengkui Zhang, Marcello Clerici, Rui Gao, Maarten van Dinther, Titia K Sixma, Huizhe Huang, Long Zhang, Peter Ten Dijke
SMAD4 is a common intracellular effector for TGF-β family cytokines, but the mechanism by which its activity is dynamically regulated is unclear. We demonstrated that ubiquitin-specific protease (USP) 4 strongly induces activin/BMP signaling by removing the inhibitory monoubiquitination from SMAD4. This modification was triggered by the recruitment of the E3 ligase, SMURF2, to SMAD4 following ligand-induced regulatory (R)-SMAD-SMAD4 complex formation. Whereas the interaction of the negative regulator c-SKI inhibits SMAD4 monoubiquitination, the ligand stimulates the recruitment of SMURF2 to the c-SKI-SMAD2 complex and triggers c-SKI ubiquitination and degradation...
May 3, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28465321/programmed-mitophagy-is-essential-for-the-glycolytic-switch-during-cell-differentiation
#20
Lorena Esteban-Martínez, Elena Sierra-Filardi, Rebecca S McGreal, María Salazar-Roa, Guillermo Mariño, Esther Seco, Sylvère Durand, David Enot, Osvaldo Graña, Marcos Malumbres, Ales Cvekl, Ana María Cuervo, Guido Kroemer, Patricia Boya
Retinal ganglion cells (RGCs) are the sole projecting neurons of the retina and their axons form the optic nerve. Here, we show that embryogenesis-associated mouse RGC differentiation depends on mitophagy, the programmed autophagic clearance of mitochondria. The elimination of mitochondria during RGC differentiation was coupled to a metabolic shift with increased lactate production and elevated expression of glycolytic enzymes at the mRNA level. Pharmacological and genetic inhibition of either mitophagy or glycolysis consistently inhibited RGC differentiation...
May 2, 2017: EMBO Journal
journal
journal
28362
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"