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Applied Biochemistry and Biotechnology

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https://www.readbyqxmd.com/read/28211009/pneumococcal-predictive-proteins-selected-by-microbial-genomic-approach-are-serotype-cross-reactive-and-bind-to-host-extracellular-matrix-proteins
#1
Ana Paula Corrêa Argondizzo, Cláudio Marcos Rocha-de-Souza, Marta de Almeida Santiago, Ricardo Galler, Joice Neves Reis, Marco Alberto Medeiros
Streptococcus pneumoniae is a colonizer of the human nasopharynx, which accounts for most of the community-acquired pneumonia cases and can cause non-invasive and invasive diseases. Current available vaccines are serotype-specific and the use of recombinant proteins associated with virulence is an alternative to compose vaccines and to overcome these problems. In a previous work, we describe the identification of proteins in S. pneumoniae by reverse vaccinology and the genetic diversity of these proteins in clinical isolates...
February 17, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28205050/composite-electrospun-scaffold-derived-from-recombinant-fibroin-of-weaver-ant-oecophylla-smaragdina-as-cell-substratum
#2
Arunrat Khamhaengpol, Sineenat Siri
Unlike silkworm (Bombyx mori) fibroin (SF), weaver ant (Oecophylla smaragdina) fibroin (WAF) is much less studied. Due to differences in amino acid composition and protein structure, this work aimed to produce the recombinant WAF protein, designated as WAF1, and investigated on its potential application as a biomaterial for producing a cell-substratum. The composite electrospun scaffolds derived from poly(vinyl alcohol) (PVA), WAF1, and extracted SF were produced by electrospinning. SEM images revealed non-woven and smooth fibers of PVA, PVA-WAF1, and PVA-SF scaffolds with the average diameters of 204...
February 15, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28205049/construction-of-potent-recombinant-strain-through-intergeneric-protoplast-fusion-in-endophytic-fungi-for-anticancerous-enzymes-production-using-rice-straw
#3
Mervat Morsy Abbas Ahmed El-Gendy, Salha Hassan Mastour Al-Zahrani, Ahmed Mohamed Ahmed El-Bondkly
Among all fungal endophytes isolates derived from different ethno-medical plants, the hyper-yield L-asparaginase and L-glutaminase wild strains Trichoderma sp. Gen 9 and Cladosporium sp. Gen 20 using rice straw under solid-state fermentation (SSF) were selected. The selected strains were used as parents for the intergeneric protoplast fusion program to construct recombinant strain for prompt improvement production of these enzymes in one recombinant strain. Among 21 fusants obtained, the recombinant strain AYA 20-1, with 2...
February 15, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28194718/employing-central-composite-design-for-evaluation-of-biomass-production-by-fusarium-venenatum-in-vivo-antioxidant-and-antihyperlipidemic-properties
#4
Asha Byju Thomas, Trupti Dattatray Shetane, Ranu Goutam Singha, Rabindra K Nanda, Sushilkumar S Poddar, Ajinath Shirsat
The present study deals with the cost effective production of biomass from Fusarium venenatum using different carbon sources (cane sugar, brown sugar, malt and fructose). Optimization of selected carbon sources and seed size using Central Composite Response Surface Design (CCRSD) indicated that sucrose (1.64 g/100 mL) and seed size (10% v/v) were optimal in maximizing biomass yield (0.5602 g/100 mL, p < 0.0001) and protein yield (49.99%, p < 0.01) of Fusarium venenatum. The acetonitrile and methanolic extracts of biomass showed promising antioxidant activity (DPPH assay, 59...
February 13, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28194717/metal-chelate-immobilization-of-lipase-onto-polyethylenimine-coated-mcm-41-for-apple-flavor-synthesis
#5
Armin Sadighi, Seyed Farshad Motevalizadeh, Morteza Hosseini, Ali Ramazani, Lena Gorgannezhad, Hamid Nadri, Behnaz Deiham, Mohammad Reza Ganjali, Abbas Shafiee, Mohammad Ali Faramarzi, Mehdi Khoobi
An enzyme immobilized on a mesoporous silica nanoparticle can serve as a multiple catalyst for the synthesis of industrially useful chemicals. In this work, MCM-41 nanoparticles were coated with polyethylenimine (MCM-41@PEI) and further modified by chelation of divalent metal ions (M = Co(2+), Cu(2+), or Pd(2+)) to produce metal-chelated silica nanoparticles (MCM-41@PEI-M). Thermomyces lanuginosa lipase (TLL) was immobilized onto MCM-41, MCM-41@PEI, and MCM-41@PEI-M by physical adsorption. Maximum immobilization yield and efficiency of 75 ± 3...
February 13, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28185056/chemical-cell-lysis-system-applicable-to-lab-on-a-disc
#6
Dayeseul Lim, Jae Chern Yoo
The design and fabrication of a heating system has been a significant challenge in implementing chemical lysis on a lab-on-a-disc (LOD). The proposed system contains a sample inlet, phase change material (PCM) array, heating chamber, and valve in a single disc, providing cost-effective, rapid, and fully automated chemical cell lysis. Compared to the conventional cell lysis system, our cell lysis system has many advantages, such as a compact structure that is easily integrated into the LOD and reduced processing time and labor...
February 9, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28185055/improving-pullulanase-catalysis-via-reversible-immobilization-on-modified-fe3o4-polydopamine-nanoparticles
#7
Jianfeng Wang, Zhongmei Liu, Zhemin Zhou
To improve the catalysis of pullulanase from Anoxybacillus sp.WB42, Fe3O4@polydopamine nanoparticles (Fe3O4@PDA) were prepared and modified with functional groups for immobilization of pullulanases via covalent binding or ionic adsorption. Immobilized pullulanases had lower thermal stability than that of free pullulanase, whereas their catalysis depended on the surface characteristics of nanoparticles. As for covalent immobilization of pullulanases onto Fe3O4@PDA derivatives, the spacer grafted onto Fe3O4@PDA made the catalytic efficiency of pullulanase increase up to the equivalence of free enzyme but dramatically reduced the pullulanase thermostability...
February 9, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28185054/identification-of-new-lead-molecules-against-ube2nl-enzyme-for-cancer-therapy
#8
Vishwanath Ramatenki, Ramakrishna Dumpati, Rajender Vadija, Santhiprada Vellanki, Sarita Rajender Potlapally, Rohini Rondla, Uma Vuruputuri
Cancer is characterized by abnormal growth of cells. Targeting ubiquitin proteins in the discovery of new anticancer therapeutics is an attractive strategy. The present study uses the structure-based drug discovery methods to identify new lead structures, which are selective to the putative ubiquitin-conjugating enzyme E2N-like (UBE2NL). The 3D structure of the UBE2NL was evaluated using homology modeling techniques. The model was validated using standard in silico methods. The hydrophobic pocket of UBE2NL that aids in binding with its natural receptor ubiquitin-conjugating enzyme E2 variant (UBE2V) was identified through protein-protein docking study...
February 9, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28181191/sea-buckthorn-leaf-extract-inhibits-glioma-cell-growth-by-reducing-reactive-oxygen-species-and-promoting-apoptosis
#9
Sung-Jo Kim, Eunmi Hwang, Sun Shin Yi, Ki Duk Song, Hak-Kyo Lee, Tae-Hwe Heo, Sang-Kyu Park, Yun Joo Jung, Hyun Sik Jun
Hippophae rhamnoides L., also known as sea buckthorn (SBT), possesses a wide range of biological and pharmacological activities. However, the underlying mechanism is largely unknown. The present study examined whether SBT leaf extract could inhibit proliferation and promote apoptosis of rat glioma C6 cells. The results revealed that the treatment with SBT leaf extract inhibited proliferation of rat C6 glioma cells in a dose-dependent manner. SBT-induced reduction of C6 glioma cell proliferation and viability was accompanied by a decrease in production of reactive oxygen species (ROS), which are critical for the proliferation of tumor cells...
February 8, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28176140/prospecting-agro-waste-cocktail-supplementation-for-cellulase-production-by-a-newly-isolated-thermophilic-b-licheniformis-2d55
#10
Muinat Olanike Kazeem, Umi Kalsom Md Shah, Azhari Samsu Baharuddin, Nor' Aini AbdulRahman
Bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. The cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for biofuel production. Among three potential bacteria examined, Bacillus licheniformis 2D55 (accession no. KT799651) was found to produce the highest cellulolytic activity (CMCase 0.33 U/mL and FPase 0...
February 7, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28161867/towards-understanding-the-catalytic-mechanism-of-human-paraoxonase-1-experimental-and-in-silico-mutagenesis-studies
#11
Rajan K Tripathy, Geetika Aggarwal, Priyanka Bajaj, Deepika Kathuria, Prasad V Bharatam, Abhay H Pande
Human paraoxonase 1 (h-PON1) is a ~45-kDa serum enzyme that can hydrolyze a variety of substrates, including organophosphate (OP) compounds. It is a potential candidate for the development of antidote against OP poisoning in humans. However, insufficient OP-hydrolyzing activity of native enzyme affirms the urgent need to develop improved variant(s) having enhanced OP-hydrolyzing activity. The crystal structure of h-PON1 remains unsolved, and the molecular details of how the enzyme catalyses hydrolysis of different types of substrates are also not clear...
February 4, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28161866/utilization-of-agro-industry-residue-for-rhamnolipid-production-by-p-aeruginosa-amb-as7-and-its-application-in-chromium-removal
#12
Mariaamalraj Samykannu, Anant Achary
Coconut oil sludge and oil cake was utilized as carbon source for biosurfactant production by Pseudomonas aeruginosa AMB AS7. The results of optimization study revealed that 1.5% (w/v) of coconut oil cake, 2% (w/v) of coconut oil sludge, pH 7.2, 37 °C, and 120 rpm were the optimum conditions for biosurfactant production. The yield coefficient of biosurfactant on biomass (Y P/X ) was 1.29 g/g. Besides, the results indicated that aeration of 0.5 vvm and agitation of 450 rpm in bioreactor resulted in high volumetric productivity of biosurfactant (r p ) and specific product formation rate (q p ) of 0...
February 4, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28160134/bacterial-and-fungal-proteolytic-enzymes-production-catalysis-and-potential-applications
#13
REVIEW
Ronivaldo Rodrigues da Silva
Submerged and solid-state bioprocesses have been extensively explored worldwide and employed in a number of important studies dealing with microbial cultivation for the production of enzymes. The development of these production technologies has facilitated the generation of new enzyme-based products with applications in pharmaceuticals, food, bioactive peptides, and basic research studies, among others. The applicability of microorganisms in biotechnology is potentiated because of their various advantages, including large-scale production, short time of cultivation, and ease of handling...
February 3, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28155169/nanosilicalites-as-support-for-%C3%AE-glucosidases-covalent-immobilization
#14
Y Carvalho, J M A R Almeida, P N Romano, K Farrance, P Demma Carà, N Pereira, J A Lopez-Sanchez, E F Sousa-Aguiar
Many different materials have been tested for β-glucosidases immobilization. Such materials, however, often show a poor activity related to a low surface area of the support or even enzyme hindrance caused by entrapment inside porous matrix. In this context, the use of nanosized zeolites as enzymes support is quite new and may be an interesting alternative. The present work evaluates the immobilization of β-glucosidases in nanosized silicalites by covalent coupling. The new biocatalyst was able to convert 100% of cellobiose into glucose in 18 h at 50 °C and pH 5, retaining 85% of its activity after five cycles of reuse...
February 2, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28155168/expression-and-characteristics-of-an-endoglucanase-from-trichoderma-atroviride-taegii-in-saccharomyces-cerevisiae
#15
Xiao-Mei Huang, Qing-Qing Li, Xiu-Ling Chen, Jin-Xia Fan, Xiu-Hong Xu, Xu-Dong Sun, Dong-Yu Li, Hong-Xiao Zhao
Endoglucanase secreted by the fungus Trichoderma atroviride is a kind of cellulase. An endoglucanase gene egII was cloned from T. atroviride AS3.3013 and expressed in Saccharomyces cerevisiae INVScI. The open reading frame of the egII gene was composed of 1257 bp, encoding 418 amino acids with a molecular weight of 44.23 kDa plus a signal peptide of 21 amino acids. Based on sequence similarity, TaEGII belonged to the glycosyl hydrolase family 5. Expression of the egII gene in T. atroviride AS3.3013 can be induced by microcrystalline cellulose (MCC), bran, carboxymethyl cellulose (CMC), rice straw, and corn stalk but is inhibited by glucose...
February 2, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28155167/inhibition-of-snake-venom-metalloproteinase-by-%C3%AE-lactoglobulin-peptide-from-buffalo-bubalus-bubalis-colostrum
#16
Ashok Arpitha, M Sebastin Santhosh, A C Rohit, K S Girish, D Vinod, H S Aparna
Bioactive peptide research has experienced considerable therapeutic interest owing to varied physiological functions, efficacy in excretion, and tolerability of peptides. Colostrum is a rich natural source of bioactive peptides with many properties elucidated such as anti-thrombotic, anti-hypertensive, opioid, immunomodulatory, etc. In this study, a variant peptide derived from β-lactoglobulin from buffalo colostrum was evaluated for the anti-ophidian property by targeting snake venom metalloproteinases. These are responsible for rapid local tissue damages that develop after snakebite such as edema, hemorrhage, myonecrosis, and extracellular matrix degradation...
February 2, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28150192/establishment-of-bioprocess-for-synthesis-of-nicotinamide-by-recombinant-escherichia-coli-expressing-high-molecular-mass-nitrile-hydratase
#17
Zhe Wang, Zhongmei Liu, Wenjing Cui, Zhemin Zhou
Application of engineered bacteria expressing nitrile hydratase for the production of amide is getting tremendous attention due to the rapid development of recombinant DNA technique. This study evaluated the effect of 3-cyanopyridine concentrations on nicotinamide production using recombinant Escherichia coli strain (BAG) expressing high-molecular-mass nitrile hydratase from Rhodococcus rhodochrous J1, and established proper process of whole-cell catalysis of 3-cyanopyridine and high cell-density cultivation...
February 1, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28138930/recombinant-3-hydroxy-3-methyl-glutaryl-coa-reductase-from-candida-glabrata-rec-cghmgr-obtained-by-heterologous-expression-as-a-novel-therapeutic-target-model-for-testing-synthetic-drugs
#18
Dulce Andrade-Pavón, Roberto I Cuevas-Hernández, José G Trujillo-Ferrara, César Hernández-Rodríguez, J Antonio Ibarra, Lourdes Villa-Tanaca
The enzyme 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGR) is a glycoprotein of the endoplasmic reticulum that participates in the mevalonate pathway, the precursor of cholesterol in human and ergosterol in fungi. This enzyme has three domains: transmembrane, binding, and soluble. In this study, we expressed and purified the soluble fraction of the HMGR enzyme from Candida glabrata (CgHMGR) in an Escherichia coli heterologous system and used it as a model for studying its inhibitory activity. The soluble fraction of CgHMGR was fused to the maltose binding protein (MBP), purified, and characterized...
January 30, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28138929/self-crosslinking-of-silk-fibroin-using-h2o2-horseradish-peroxidase-system-and-the-characteristics-of-the-resulting-fibroin-membranes
#19
Buguang Zhou, Ping Wang, Li Cui, Yuanyuan Yu, Chao Deng, Qiang Wang, Xuerong Fan
Silk fibroin has been widely used in biomedical and clinical fields owing to its good biocompatibility. In the present work, self-crosslinking of fibroin molecules was carried out using the hydrogen peroxide (H2O2)-horseradish peroxidase system, followed by preparation of the fibroin membranes, aiming at improving the mechanical property of fibroin-based material and expanding its applications. P-Hydroxyphenylacetamide (PHAD), as the model compound of tyrosine residues in fibroins, was used to investigate the possibility of horseradish peroxidase (HRP)-catalyzed crosslinking...
January 30, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28138928/analysis-of-pseudomonas-aeruginosa-pao1-biofilm-protein-profile-after-exposure-to-n-butanolic-cyclamen-coum-extract-alone-and-in-combination-with-ciprofloxacin
#20
Morvarid Shafiei, Ahya Abdi-Ali, Fereshteh Shahcheraghi, Hojatollah Vali, Hossein Shahbani Zahiri, Kambiz Akbari Noghabi
Pseudomonas aeruginosa biofilm-related infections are the major cause of premature death in cystic fibrosis patients. Strategies to induce biofilm dispersal are of interest, because of their potential in preventing biofilm-related infections. Our previous work demonstrated that n-butanolic Cyclamen coum extract with ciprofloxacin could eliminate 1- and 3-day-old P. aeruginosa PAO1 biofilms. To gain new insights into the role of C. coum extract and its synergistic effect with ciprofloxacin in eliminating P. aeruginosa PAO1 biofilms, two-dimensional gel electrophoresis (2-DE) in combination with mass spectrometry-based protein identification were used...
January 30, 2017: Applied Biochemistry and Biotechnology
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