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Applied Biochemistry and Biotechnology

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https://www.readbyqxmd.com/read/27933484/dna-as-a-target-for-anticancer-phen-imidazole-pd-ii-complexes
#1
Maryam Heydari, Mahboube Eslami Moghadam, AliAkbar Tarlani, Hossein Farhangian
Imidazole ring is a known structure in many natural or synthetic drug molecules and its metal complexes can interact with DNA and do the cleavage. Hence, to study the influence of the structure and size of the ligand on biological behavior of metal complexes, two water-soluble Pd(II) complexes of phen and FIP ligands (where phen is 1,10-phenanthroline and FIP is 2-(Furan-2-yl)-1H-Imidazo[4,5-f][1, 10]phenanthroline) with the formula of [Pd(phen)(FIP)](NO3)2 and [Pd(FIP)2]Cl2, that were activated against chronic myelogenous leukemia cell line, K562, were selected...
December 8, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27933483/covalent-immobilization-of-human-placental-17%C3%AE-hydroxysteroid-dehydrogenase-type-1-onto-glutaraldehyde-activated-silica-coupled-with-lc-tof-ms-for-anti-cancer-drug-screening-applications
#2
Yin Bai, Wen-Di Zhou, Xian-Min Mu, Qian Zhang, Chen Yu, Bin Di, Meng-Xiang Su
Human 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1), a potential target in breast cancer prevention and therapy, was extracted from human placenta and immobilized on nonporous silica (∼5 μm) with a covalent method for the first time. The optimum initial enzyme concentration and immobilization time during the immobilization process were 0.42 mg mL(-1) and 12 h, repectively. The binding was confirmed by scanning electron microscope (SEM) and infrared spectroscopy (FT-IR). It could improve the pH, thermal and storage stability compared to free enzyme...
December 8, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27933482/effect-of-linker-length-and-flexibility-on-the-clostridium-thermocellum-esterase-displayed-on-bacillus-subtilis-spores
#3
Huayou Chen, Bangguo Wu, Tianxi Zhang, Jinru Jia, Jian Lu, Zhi Chen, Zhong Ni, Tianwei Tan
In fusion protein design strategies, the flexibility and length of linkers are important parameters affecting the bioactivity of multifunctional proteins. A series of fusion proteins with different linkers were constructed. The effect of temperature, pH, and organic solvents was investigated on the enzymatic activity. Fusion proteins with P1(PTPTPT) and P2((PTPTPT)2) linkers remained highly active with wide temperature range. At pH 9.6, the relative activity of fusion proteins with (PTPTPT)2 and S2(EGKSSGSGSESKST) linkers was 70 and 62 % (1...
December 8, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27928761/proteomic-analysis-reveals-the-contribution-of-tgf%C3%AE-smad4-signaling-pathway-to-cell-differentiation-during-planarian-tail-regeneration
#4
Xiaoguang Chen, Cunshuan Xu
After planarian tail is cut off, posterior end of the remaining fragment will regenerate a new tail within about 1 week. However, many details of this process remain unclear up to date. For this reason, we performed the dynamic proteomic analysis of the regenerating tail fragments at 6, 12, 24, 72, 120, and 168 h post-amputation (hpa). Using two-dimensional electrophoresis (2-DE) in combination with MALDI-TOF-TOF/MS analysis, a total of 1088 peptides were identified as significantly changed between tail-cutting groups and 0-h group, 482 of which have identifiable protein names...
December 7, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27924498/isolation-and-characterization-of-acrylamidase-from-arthrobacter-sp-dbv1-and-its-ability-to-biodegrade-acrylamide
#5
Dattatray K Bedade, Rekha S Singhal
Although acrylamide finds diverse industrial applications, its presence in the environment is hazardous due to its carcinogenic, neurotoxic, and teratogenic properties. In spite of the general toxicity of acrylamide in the monomer form, some microorganisms are able to use it as a source of energy by catabolizing it to ammonia and acrylic acid by means of acrylamidase (EC 3.5.1.4). The present work reports on a novel soil isolate as an acrylamide-degrading bacteria. Based on biochemical characterization and 16S ribosomal RNA (rRNA) gene sequence, the bacterial strain was identified as Gram-positive Arthrobacter sp...
December 6, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27917441/size-selective-harvesting-of-extracellular-vesicles-for-strategic-analyses-towards-tumor-diagnoses
#6
Chihiro Manri, Takahide Yokoi, Hirokazu Nishida
Extracellular vesicles (EV), typified by exosomes or microvesicles, are expected to be effective diagnostic markers for cancers. The sizes of the vesicles range from 20 to 1000 nm, but the size-dependent variations of the contents of EVs are still poorly understood. We succeeded in the size-selective harvesting of the vesicles by utilizing the molecular weight-dependent characteristics of a variety of polyethylene glycols (PEG) as precipitating reagents and analyzed the antigens displayed on the surfaces of the vesicles and the miRNAs included in the vesicles from each size group...
December 5, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27917440/solid-flocculation-and-emulsifying-activities-of-the-lipopolysaccharide-produced-by-trichosporon-mycotoxinivorans-cla2
#7
Vitor S Domingues, Andrea S Monteiro, Gabriella Freitas Ferreira, Vera L Santos
The objective of this study was to evaluate the capability of a lipopolysaccharide, produced by Trichosporon mycotoxinivorans CLA2 using residue of biodiesel processing, to flocculate two different solids in suspension. In addition, the emulsifying activity and the stability of this lipopolysaccharide in response to pH and temperature variations and in the presence of some electrolytes were evaluated. The lipopolysaccharide was used in concentrations ranging from 20 to 80 mgL(-1) to flocculate 100 gL(-1) of kaolin and 50 gL(-1) of charcoal...
December 5, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27914022/alkaline-protease-production-from-brevibacterium-luteolum-mtcc-5982-under-solid-state-fermentation-and-its-application-for-sulfide-free-unhairing-of-cowhides
#8
R Renganath Rao, M Vimudha, N R Kamini, M K Gowthaman, B Chandrasekran, P Saravanan
Enzyme-based unhairing in replacement of conventional lime sulfide system has been attempted as an alternative for tackling pollution. The exorbitant cost of enzyme and the need for stringent process control need to be addressed yet. This study developed a mechanism for regulated release of protease from cheaper agro-wastes, which overcomes the necessity for stringent process control along with total cost reduction. The maximum protease activity of 1193.77 U/g was obtained after 96 h of incubation with 15% inoculum of the actinomycete strain Brevibacterium luteolum (MTCC 5982) under solid-state fermentation (SSF)...
December 2, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27914021/surface-modified-cobalt-ferrite-nanoparticles-for-rapid-capture-detection-and-removal-of-pathogens-a-potential-material-for-water-purification
#9
Raghvendra A Bohara, Nanasaheb D Throat, Nayeem A Mulla, Shivaji H Pawar
Enteric infections resulting from the consumption of contaminated drinking water, inadequate supply of water for personal hygiene, and poor sanitation take a heavy toll worldwide, and developing countries are the major sufferers. Consumption of microbiologically contaminated water leads to diseases such as amoebiasis, cholera, shigellosis, typhoid, and viral infections leading to gastroenteritis and hepatitis B. The present investigation deals with the development of effective method to capture and eliminate microbial contamination of water and improve the quality of water and thus decreasing the contaminated waterborne infections...
December 2, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27914020/characterization-of-xylanase-and-cellulase-produced-by-a-newly-isolated-aspergillus-fumigatus-n2-and-its-efficient-saccharification-of-barley-straw
#10
Chaoyang Lin, Zhicheng Shen, Wensheng Qin
Aspergillus fumigatus N2 was isolated from decaying wood. This strain produces extracellular xylanases and cellulases. The highest xylanase (91.9 U/mL) and CMCase (5.61 U/mL) activity was produced when 1% barley straw was used as the carbon source. The optimum pH and temperature for xylanase activity were 6.0 and 65 °C, respectively. CMCase revealed maximum activity at pH 4.0 and in the range of 65 °C. The FPase was optimally active at pH 5.0 and 60 °C. The zymograms produced by the SDS-PAGE resolution of the crude enzymes indicated that multiple enzymes were secreted into the fermentation supernatant...
December 2, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27914019/erratum-to-hydrophobic-interaction-between-domain-i-of-albumin-and-b-chain-of-detemir-may-support-myristate-dependent-detemir-albumin-binding
#11
Sadaf Fatima, Priyankar Sen, P Sneha, C George Priyadoss
No abstract text is available yet for this article.
December 2, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27914018/encapsulation-of-%C3%AE-sitosterol-in-polyurethane-by-sol-gel-electrospinning
#12
Musarat Amina, Touseef Amna, Nawal Al-Musayeib, Sami A Zabin, M Shamshi Hassan, Myung-Seob Khil
Pristine β-sitosterol or in combination with other phytosterols is utilized in an array of enriched commercial foods. Considering the presence of β-sitosterol in different functional foods and its potential role in prevention and cure of neurodegenerative diseases, the aims of our investigation were to encapsulate β-sitosterol in nanofibers and to estimate influence of β-sitosterol on proliferation of fibroblasts. Electrospun nanofibers have widely been used as scaffolds to mimic natural extracellular matrix...
December 2, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27909876/sonochemical-effect-on-activity-and-conformation-of-commercial-lipases
#13
Shamraja S Nadar, Virendra K Rathod
The enzyme under lower-intensity ultrasonic irradiation leads to favourable conformational changes, thereby enhancing its activity. The augmentation of activity of ultrasound-treated enzyme is strongly dependent on ultrasound intensity, duty cycle and exposure time, which was investigated for commercial lipases. Thermomyces lanuginosus (TL) lipase showed a 1.3-fold enhanced activity after irradiating at 22 kHz and 11.38 W cm(-2) with 50 % duty cycle for 25-min ultrasonic treatment and 1.5-fold enhanced activity was observed for lipozyme (candida antarctica lipase B (CALB)) lipase, at 22 kHz and 15...
December 1, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27900666/improved-production-of-thermostable-cellulase-from-thermoascus-aurantiacus-rckk-by-fermentation-bioprocessing-and-its-application-in-the-hydrolysis-of-office-waste-paper-algal-pulp-and-biologically-treated-wheat-straw
#14
Kavish Kumar Jain, Sandeep Kumar, Deepa Deswal, Ramesh Chander Kuhad
Thermostable cellulases have wide variety of applications and distinctive advantages, but their low titer becomes the hurdle in their commercialization. In the present work, an assessment of optimum levels of significant factors (temperature, moisture ratio, inoculum size, and ammonium sulfate) and the effect of their interactions on production of thermostable CMCase, FPase, and β-glucosidase by Thermoascus aurantiacus RCKK under solid-state fermentation (SSF) was carried out using central composite design (CCD) of response surface methodology (RSM)...
November 30, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27900665/use-of-capillary-electrophoresis-to-study-the-binding-interaction-of-aptamers-with-wild-type-k103n-and-double-mutant-k103n-y181c-hiv-1-rt-studying-the-binding-interaction-of-wild-type-k103n-and-double-mutant-k103n-y181c-hiv-1-rt-with-aptamers-by-performing
#15
Niran Aeksiri, Chompunuch Warakulwit, Supa Hannongbua, Sasimanas Unajak, Kiattawee Choowongkomon
A number of nucleic acid aptamers with high affinities to human immunodeficiency virus reverse transcriptase (HIV-1 RT) are currently known. They can potentially be developed as broad-spectrum antiviral drugs, but there is little known about their binding interaction with mutant HIV-1 RT. Therefore, we utilized non-equilibrium capillary electrophoresis of equilibrium mixture (NECEEM) to study the interaction of three HIV-1 RTs (wild type, K103N, and double mutant (K103N/Y181C)) with RT1t49 and RT12 aptamers...
November 30, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27900664/improvement-production-of-hyaluronic-acid-by-streptococcus-zooepidemicus-in-sugarcane-molasses
#16
Nicole Caldas Pan, Hanny Cristina Braga Pereira, Maria de Lourdes Corradi da Silva, Ana Flora Dalberto Vasconcelos, Maria Antonia Pedrine Colabone Celligoi
Microbial hyaluronic acid (HA) production has been preferred rather than extraction from animal tissue for medical and cosmetic applications. In this context, to obtain an economically competitive HA production by Streptococcus zooepidemicus, culture conditions were studied to improve the polymer production in sugarcane molasses. The highest HA production by S. zooepidemicus ATCC 39920 achieved was 2.825 g. L(-1) in a 4.5 L bioreactor with controlled pH (8.0) and medium containing molasses (85.35 g.L(-1) total sugar) pretreated with activated charcoal and yeast extract (50 g...
November 30, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27900555/x-ray-crystallography-as-a-tool-to-determine-three-dimensional-structures-of-commercial-enzymes-subjected-to-treatment-in-pressurized-fluids
#17
REVIEW
Mirian Cristina Feiten, Marco Di Luccio, Karine F Santos, Débora de Oliveira, J Vladimir Oliveira
The study of enzyme function often involves a multi-disciplinary approach. Several techniques are documented in the literature towards determining secondary and tertiary structures of enzymes, and X-ray crystallography is the most explored technique for obtaining three-dimensional structures of proteins. Knowledge of three-dimensional structures is essential to understand reaction mechanisms at the atomic level. Additionally, structures can be used to modulate or improve functional activity of enzymes by the production of small molecules that act as substrates/cofactors or by engineering selected mutants with enhanced biological activity...
November 29, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27889870/secretory-expression-purification-characterization-and-application-of-an-aspergillus-oryzae-prolyl-aminopeptidase-in-bacillus-subtilis
#18
Kai-Dao Wang, Ke-Hong Wang, Nan-Di Zhou, Ya-Ping Tian
cDNA coding a prolyl aminopeptidase (PAP) was cloned from Aspergillus oryzae and over expressed in Bacillus subtilis with a 6×His tag in N-terminus. The recombinant prolyl aminopeptidase was secreted to extracellular by adding 2 mM CaCl2 and 5% D-sorbitol in TB medium; the enzyme activity in fermented supernatant increased from 7.2 to 41.5 U mL(-1). It has been purified 4.3-fold through Ni-chelating affinity chromatography with a recovery of 47.3%. The purified enzyme is stable below 50 °C and within pH 6-11, and with the highest activity at pH 7...
November 26, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27882483/effect-of-co2-concentration-on-growth-and-biochemical-composition-of-newly-isolated-indigenous-microalga-scenedesmus-bajacalifornicus-bbklp-07
#19
Lakkanagouda Patil, Basappa Kaliwal
Photosynthetic mitigation of CO2 through microalgae is gaining great importance due to its higher photosynthetic ability compared to plants, and the biomass can be commercially exploited for various applications. CO2 fixation capability of the newly isolated freshwater microalgae Scenedesmus bajacalifornicus BBKLP-07 was investigated using a 1-l photobioreactor. The cultivation was carried at varying concentration of CO2 ranging from 5 to 25%, and the temperature and light intensities were kept constant. A maximum CO2 fixation rate was observed at 15% CO2 concentration...
November 23, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27882482/immobilization-and-stabilization-of-beta-xylosidases-from-penicillium-janczewskii
#20
César Rafael Fanchini Terrasan, Maria Romero-Fernández, Alejandro H Orrego, Sandro Martins Oliveira, Benevides Costa Pessela, Eleonora Cano Carmona, José Manuel Guisan
β-Xylosidases are critical for complete degradation of xylan, the second main constituent of plant cell walls. A minor β-xylosidase (BXYL II) from Penicillium janczewskii was purified by ammonium sulfate precipitation (30% saturation) followed by DEAE-Sephadex chromatography in pH 6.5 and elution with KCl. The enzyme presented molecular weight (MW) of 301 kDa estimated by size exclusion chromatography. Optimal activity was observed in pH 3.0 and 70-75 °C, with higher stability in pH 3.0-4.5 and half-lives of 11, 5, and 2 min at 65, 70, and 75 °C, respectively...
November 23, 2016: Applied Biochemistry and Biotechnology
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