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Applied Biochemistry and Biotechnology

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https://www.readbyqxmd.com/read/28647795/investigation-of-chlorella-vulgaris-utex-265-cultivation-under-light-and-low-temperature-stressed-conditions-for-lutein-production-in-flasks-and-the-coiled-tree-photo-bioreactor-ctpbr
#1
Mengyue Gong, Amarjeet Bassi
Lutein has an increasing share in the pharmaceutical and nutraceutical market due to its benefits to eye health. Microalgae may be a potential source for lutein production while the expense limits the commercialization. In this study, a coiled tubular tree photobioreactor (CTPBR) design was investigated for cultivating the cold tolerant microalgae Chlorella vulgaris UTEX 265 under various conditions for lutein production. The influence and interaction of light irradiance strength, lighting cycle, and temperature on microalgae and lutein production efficiency at low temperature range were also studied in flasks via response surface method (RSM)...
June 24, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28643121/a-study-on-the-expression-of-genes-involved-in-carotenoids-and-anthocyanins-during-ripening-in-fruit-peel-of-green-yellow-and-red-colored-mango-cultivars
#2
G R Karanjalker, K V Ravishankar, K S Shivashankara, M R Dinesh, T K Roy, D V Sudhakar Rao
Mango (Mangiferaindica L.) fruits are generally classified based on peel color into green, yellow, and red types. Mango peel turns from green to yellow or red or retain green colors during ripening. The carotenoids and anthocyanins are the important pigments responsible for the colors of fruits. In the present study, peels of different colored cultivars at three ripening stages were characterized for pigments, colors, and gene expression analysis. The yellow colored cultivar "Arka Anmol" showed higher carotenoid content, wherein β-carotene followed by violaxanthin were the major carotenoid compounds that increased during ripening...
June 22, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28624999/in-vitro-biotransformation-safety-and-chemopreventive-action-of-novel-8-methoxy-purine-2-6-dione-derivatives
#3
Małgorzata Anna Marć, Enrique Domínguez-Álvarez, Karolina Słoczyńska, Paweł Żmudzki, Grażyna Chłoń-Rzepa, Elżbieta Pękala
Metabolic stability, mutagenicity, antimutagenicity, and the ability to scavenge free radicals of four novel 8-methoxy-purine-2,6-dione derivatives (compounds 1-4) demonstrating analgesic and anti-inflammatory properties were determined. Metabolic stability was evaluated in Cunninghamella and microsomal models, mutagenic and antimutagenic properties were assessed using the Ames and the Vibrio harveyi tests, and free radical scavenging activity was evaluated with 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay...
June 17, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28624998/efficient-conversion-of-fructose-based-biomass-into-lipids-with-trichosporon-fermentans-under-phosphate-limited-conditions
#4
Ruiqi Bao, Xiangying Wu, Sasa Liu, Tongzhou Xie, Chenxu Yu, Xinping Lin
Limiting nitrogen supply has been routinely used as the master regulator to direct lipid biosynthesis. However, this strategy does not work with nitrogen-rich substrates, such as Jerusalem artichoke (JA), a fructose-based biomass, while it is difficult to obtain a high carbon-to-nitrogen (C/N) molar ratio. In this study, an alternative strategy to promote lipid accumulation by the oleaginous yeast Trichosporon fermentans CICC 1368 was developed by limiting phosphorous supply, and this strategy was implemented with JA hydrolysate as substrate...
June 17, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28624997/expression-and-purification-of-cytochrome-p450-55b1-from-chlamydomonas-reinhardtii-and-its-application-in-nitric-oxide-biosensing
#5
Bin Gong, Xiaosheng Liang, Yong Li, Qian Xiao, Panchun Yang, Yunhua Wu
Cytochrome P450 55B1 from Chlamydomonas reinhardtii is reported to function as a nitric oxide reductase (NOR). Here, we expressed the cytochrome P450 55B1 gene with an HIS-tag in E scherichia coli using a pET28a vector. The native protein was produced at a level of 1.59 μmol/g of total protein, with approximately 85% of the P450 being soluble. The CYP55B1 protein was characterized spectrally and purified by a HIS-trap column. This procedure allowed recovery of 45% of the expressed protein and CYP55B1 with a specific content of 0...
June 17, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28624996/exploring-the-bioelectrochemical-characteristics-of-activated-sludge-using-cyclic-voltammetry
#6
Dena Z Khater, K M El-Khatib, Rabeay Y A Hassan
Due to the potential interest, bioelectrochemical responses of activated sludge using the three-electrode system are tested. From the cyclic voltammograms, the oxidation current output is increasing due to incubation time increase, whereas 5, 25 and 39.33 μA are obtained after 3, 72 and 96 h, respectively. Changing the working electrode from glassy carbon to carbon paste led to the increase in the electrochemical signal from 0.3 to be 3.72 μA. On the other hand, the use of the lipophilic redox mediator (2,6-dichlorophenolindophenol (DCIP)) amplified the oxidation current to reach 19...
June 17, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28612271/overexpression-of-dnaj-like-chaperone-enhances-carotenoid-synthesis-in-chlamydomonas-reinhardtii
#7
Tateki Morikawa, Yusuke Uraguchi, Shohei Sanda, Satoshi Nakagawa, Shigeki Sawayama
Production of functional carotenoids using microalgae may facilitate the commercialization of anti-aging nutritional supplements. The green alga Chlamydomonas reinhardtii uses a non-mevalonate (MEP) pathway for isopentenyl diphosphate (IPP) synthesis. Two enzymes thought to play important roles in this MEP pathway to IPP synthesis are 1-deoxy-D-xylulose 5-phosphate synthase (DXS) and reductase (DXR). DnaJ-like chaperone (Orange protein) is thought to support phytoene synthase, a key enzyme in plant carotenoid synthesis...
June 13, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28608173/improvement-in-submergence-tolerance-of-cherry-through-regulation-of-carbohydrate-metabolism-and-plant-growth-by-pserf-and-pscipk
#8
Lixia Sheng, Xiangyi Meng, Meng Wang, Shu Zang, Liguo Feng
Cherry is an important fruit tree with delicious taste and high economic value, which have been planted worldwide. However, this species cannot withstand the presence of excessive amount of water; submergence injury sometimes occurs during cultivation of cherry and results in severe economic losses. By using a submergence-tolerant germplasm Prunus serrulata "Yimeng" and a submergence-sensitive germplasm Prunus pseudocerasus "Aihua" as test materials, this study cloned PsERF and PsCIPK, which are related to submergence tolerance in cherry, and analyzed the expression of PsERF and PsCIPK in submergence-tolerant and submergence-sensitive germplasms under submergence stress; moreover, the consistency and correlation of such expression with carbohydrate metabolism and plant growth-related genes (PsPDC, PsSUS, PsRAMY, and PsEXP) were analyzed...
June 13, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28597311/integrated-process-for-ethanol-biogas-and-edible-filamentous-fungi-based-animal-feed-production-from-dilute-phosphoric-acid-pretreated-wheat-straw
#9
Ramkumar B Nair, Maryam M Kabir, Patrik R Lennartsson, Mohammad J Taherzadeh, Ilona Sárvári Horváth
Integration of wheat straw for a biorefinery-based energy generation process by producing ethanol and biogas together with the production of high-protein fungal biomass (suitable for feed application) was the main focus of the present study. An edible ascomycete fungal strain Neurospora intermedia was used for the ethanol fermentation and subsequent biomass production from dilute phosphoric acid (0.7 to 1.2% w/v) pretreated wheat straw. At optimum pretreatment conditions, an ethanol yield of 84 to 90% of the theoretical maximum, based on glucan content of substrate straw, was observed from fungal fermentation post the enzymatic hydrolysis process...
June 8, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28593603/optimization-of-inexpensive-agricultural-by-products-as-raw-materials-for-bacitracin-production-in-bacillus-licheniformis-dw2
#10
Qin Wang, Hui Zheng, Xia Wan, Hongfeng Huang, Junhui Li, Christopher T Nomura, Changjun Wang, Shouwen Chen
Bacitracin is a broad-spectrum antibiotic used extensively as a feed additive. In this study, inexpensive agricultural by-products were used as nitrogen sources for bacitracin production. Based on both the orthogonal tests, a combination of 7% soybean meal (SBM) +2% low protein rapeseed cake (LPRC) was optimal for bacitracin production. Compared to the original formula, the titer of bacitracin increased by 20.5% reaching 910.4 U/ml in flasks. The titer of bacitracin and the ratio of bacitracin A increased by 12...
June 7, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28593602/efficient-biotransformation-of-astragaloside-iv-to-cycloastragenol-by-bacillus-sp-lg-502
#11
Liming Wang, Yan Chen
Cycloastragenol (CA), an exclusive telomerase activator, was derived from the Astragali Radix which is widely distributed in Turkey. Until now, there is no report to produce CA with effective and environment-friendly methods. Biotransformation is considered to be a promising technology. Thus, the present study was aimed to establish a biotransformation technology that could efficiently produce CA. In this paper, a microorganism, LG-502, was used to successfully transform astragaloside IV (ASI) to CA by analysis of thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC)...
June 7, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28584967/effect-of-uv-b-radiation-and-desiccation-stress-on-photoprotective-compounds-accumulation-in-marine-leptolyngbya-sp
#12
Devika Joshi, C Mohandass, Mohan Dhale
Increased awareness regarding the harmful effects of ultraviolet (UV)-B radiation has led to the search for new sources of natural UV-B protecting compounds. Mycosporine-like amino acids are one of such promising compounds found in several organisms. Cyanobacteria are ideal organisms for isolation of these compounds due to their compatibility and adaptability to thrive under harsh environmental conditions. In the following investigation, we report the production of shinorine in Leptolyngbya sp. isolated from the intertidal region...
June 6, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28584966/relations-between-moso-bamboo-surface-properties-pretreated-by-kraft-cooking-and-dilute-acid-with-enzymatic-digestibility
#13
Juan He, Caoxing Huang, Chenhuan Lai, Chen Huang, Qiang Yong
Lignocellulosics pretreatment is intended to dismantle biomass' natural recalcitrance. Chemical and morphological changes of lignocellulosic materials would occur through varying pretreatment processes. In order to figure out why the acid-pretreated and Kraft-cooked bamboo residues showed very different enzymatic digestibility, the surface properties of pretreated bamboo residues were characterized in this work. It was found that analyzed surface properties were considered to be associating with each corresponding enzymatic digestibility of the pretreated samples...
June 6, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28584965/enzymatic-hydrolysis-of-agavins-to-generate-branched-fructooligosaccharides-a-fos
#14
Alicia Huazano-García, Mercedes G López
Recently, agavins (branched neo-fructans) of short degree of polymerization have shown beneficial effects on the health of both healthy and overweight individuals. Therefore, the aim of the present work was to investigate the potential use of Agave angustifolia agavins on the generation of branched fructooligosacharides (a-FOS). A. angustifolia agavins were hydrolyzed using exo-, endo-inulinase, and a mixture of both (25 and 75%, respectively). Exo- and the inulinase mixture degraded quickly the agavins in relation to endo-inulinase treatment...
June 6, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28580528/retraction-note-to-genome-shuffling-enhances-lipase-production-of-thermophilic-geobacillus-sp
#15
Pornchanok Chalopagorn, Jittima Charoenpanich, Kiattawee Choowongkomon
No abstract text is available yet for this article.
June 3, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28577192/cloning-site-directed-mutagenesis-and-functional-analysis-of-active-residues-in-lymantria-dispar-chitinase
#16
Xiao-Jun Fan, Chun Yang, Chang Zhang, Hui Ren, Jian-Dong Zhang
Chitinases are glycosyl hydrolases that catalyze the hydrolysis of β-(1,4)-glycosidic bonds in chitin, the major structural polysaccharide presented in the cuticle and gut peritrophic matrix of insects. Two aspartate residues (D143, D145) and one tryptophan (W146) in the Lymantria dispar chitinase are highly conserved residues observed within the second conserved motif of the family 18 chitinase catalytic region. In this study, a chitinase cDNA, LdCht5, was cloned from L. dispar, and the roles of the three residues were investigated using site-directed mutagenesis and substituting them with three other amino acids...
June 2, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28577191/deletion-analysis-of-gh7-endoglucanase-gene-cel7b-promoter-region-in-a-talaromyces-cellulolyticus-ligd-disrupted-strain
#17
Tatsuya Fujii, Hiroyuki Inoue, Kazuhiko Ishikawa, Tamotsu Hoshino
Talaromyces cellulolyticus is expected to become an industrial cellulase producer. In this study, we performed deletion analysis of the promoter region of the GH7 endoglucanase gene (cel7B), which encodes one of the major cellulases, using a β-glucuronidase reporter system. To obtain strains that harbor each gene cassette at the same locus, we had to improve the homologous recombination frequency. Hence, the ligD gene, encoding DNA ligase IV, was disrupted by homologous recombination. After that, the introduced pyrF marker gene, encoding orotate phosphoribosyl transferase, was deleted by a marker recycling system...
June 2, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28573605/inhibitory-effect-of-biosynthesized-silver-nanoparticles-from-extract-of-nitzschia-palea-against-curli-mediated-biofilm-of-escherichia-coli
#18
Rajamohamed Beema Shafreen, Siddharthan Seema, Abdulazees Parveez Ahamed, Nooruddin Thajuddin, Sulaiman Ali Alharbi
Extended spectrum beta lactamase (ESBL) are emerging beta-lactamases in Gram-negative pathogens, causing serious problems in hospitalized patients worldwide. Biofilm mode of virulence has decreased the efficiency of antibiotics used for treatment against ESBL pathogens. Therefore, there is an urgent need for alternative agents such as nanoparticles that can prevent and inhibit the biofilm formation. The aim of the present study was to inhibit the biofilm formed by ESBL-producing Escherichia coli using silver nanoparticles (AgNPs) synthesized with fresh water diatom (Nitzschia palea)...
June 1, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28573604/dextran-utilization-during-its-synthesis-by-weissella-cibaria-rba12-can-be-overcome-by-fed-batch-fermentation-in-a-bioreactor
#19
Rwivoo Baruah, Barsha Deka, Niharika Kashyap, Arun Goyal
Weissella cibaria RBA12 produced a maximum of 9 mg/ml dextran (with 90% efficiency) using shake flask culture under the optimized concentration of medium components viz. 2% (w/v) of each sucrose, yeast extract, and K2HPO4 after incubation at optimized conditions of 20 °C and 180 rpm for 24 h. The optimized medium and conditions were used for scale-up of dextran production from Weissella cibaria RBA12 in 2.5-l working volume under batch fermentation in a bioreactor that yielded a maximum of 9.3 mg/ml dextran (with 93% efficiency) at 14 h...
June 1, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28573603/influence-of-culture-medium-composition-and-light-conditions-on-the-accumulation-of-bioactive-compounds-in-shoot-cultures-of-scutellaria-lateriflora-l-american-skullcap-grown-in-vitro
#20
Beata Kawka, Inga Kwiecień, Halina Ekiert
Methanolic extracts from in vitro grown Scutellaria lateriflora shoots cultured on five Murashige and Skoog (MS) medium variants supplemented with different combinations of 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA) under different light conditions (monochromatic light, white light and no light) were analysed by HPLC for three groups of metabolites: flavonoids (26 compounds), phenolic acids and their precursors (19+2) and phenylethanoid glycosides (2). The analyses revealed the presence of baicalein, baicalin, wogonin, wogonoside, 3,4-dihydroxyphenylacetic acid and verbascoside...
June 1, 2017: Applied Biochemistry and Biotechnology
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