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Journal of Cellular Biochemistry

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https://www.readbyqxmd.com/read/29024007/snp-mutations-occurring-in-thyroid-hormone-receptor-influenced-individual-susceptibility-to-triiodothyronine-molecular-dynamics-and-site-directed-mutagenesis-approaches
#1
Yaquan Liu, Kaili Qu, Ying Hai, Xin Li, Lei Zhao, Chunyan Zhao
The increasing evidences have suggested that expression of single nucleotide polymorphisms (SNP) coded thyroid hormone receptors (THR) generally are associated with individual susceptibility to chemicals. In the present research, multiple molecular dynamics simulations on four SNP mutants (G332R, T337△, G345R and G347E) were performed to investigate the structural and dynamical altering, which could lead to a binding capability variation to triiodothyronine (T3). It proved the structures of two SNP mutants (G345R and T337△) occurring in the THR proteins had experienced conformational change to a great extend, which also led to a significant decreasing in binding ability with T3...
October 11, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29023986/evaluation-of-short-and-long-term-efficacy-of-chemoradiotherapy-for-advanced-cervical-cancer-using-hsp70-protein-combined-with-multimodal-mri
#2
Yun-Xiang Qi, Kun Liu, Jun Yin, Lu Li
This study aims to evaluate the efficacy of chemoradiotherapy for advanced cervical cancer using heat shock protein 70 (HSP70) combined with multimodal MRI. The protein expressions of HSP70 in biopsy specimens obtained from 101 patients with cervical cancer were detected by immunohistochemistry. Plain MRI scan, DWI, DCE-MR and MRS were performed before and after a period of six months of chemoradiotherapy. All patients were assigned into the complete response (CR) and partial response (PR) groups. HSP70 protein expression, tumor diameter and tumor volume were lower in the CR group than in the PR group...
October 11, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29023981/4-bp-insertion-deletion-rs3783553-polymorphism-within-the-3-utr-of-il1a-contributes-to-the-risk-of-prostate-cancer-in-a-sample-of-iranian-population
#3
Mohammad Hashemi, Gholamreza Bahari, Shamim Sarhadi, Ebrahim Eskandari, Behzad Narouie, Mohsen Taheri, Saeid Ghavami
Growing evidence demonstrated the presence of an association between IL1A rs3783553 polymorphism and the risk of various cancers. We aimed to evaluate whether the 4-bp insertion/deletion polymorphism (rs3783553) within the 3' untranslated region (3'UTRs) of IL1A was associated to the risk of prostate cancer (PCa) in a sample of Iranian population. A case-control study, including 150 prostate cancer patients and 155 healthy men, was done to examine the possible association between IL1A 4-bp ins/del polymorphism and PCa risk in a sample of southeast Iranian population...
October 11, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29023945/microrna-29a-inhibits-proliferation-and-motility-of-schwannoma-cells-by-targeting-cdk6
#4
Ji Ma, Tengfei Li, Huifeng Yuan, Xinwei Han, Shaofeng Shui, Dong Guo, Lei Yan
AIM: MicroRNA-29 (miR-29)family involved in various types of cancer regulation. Although miR-29a was shown to play an inhibitory role in tumorigenesis, the effect of miR-29a expression on schwannoma cells still remains unclear. In this study, we aimed to explore the role of miR-29a in schwannoma. METHODS: The expressions of miR-29a, miR-29b and miR-29c were detected in the Schwann tissues and cell lines using qRT-PCR. The effect of miR-29a, miR-29b and miR-29c on cell viability, migration, invasion and apoptosis was tested...
October 11, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28981158/low-intensity-pulsed-ultrasound-stimulation-promotes-osteoblast-differentiation-through-hedgehog-signaling
#5
Kenichi Matsumoto, Tsuyoshi Shimo, Naito Kurio, Tatsuo Okui, Soichiro Ibaragi, Yuki Kunisada, Kyoichi Obata, Masanori Masui, Pang Pai, Yuu Horikiri, Nobuyuki Yamanaka, Masaharu Takigawa, Akira Sasaki
Low-intensity pulsed ultrasound (LIPUS) has been used as an adjunct to fracture healing therapies, but the mechanisms underlying its action are not known. We reported that sonic hedgehog (SHH) signaling was activated in osteoblasts at the dynamic remodeling site of a bone fracture. Mechanical stimulation is a crucial factor in bone remodeling, and it is related to the primary cilia as a sensor of hedgehog signaling. Here we observed that LIPUS promoted callus formation in accord with Gli2-positive cells after 14 days at the mouse femur fractured site compared with a control group...
October 5, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28981155/phospholipid-secretions-of-organ-cultured-ciliary-body
#6
Michael J Margolis, Mitchell Martinez, Jeffrey Valencia, Richard K Lee, Sanjoy K Bhattacharya
Homeostasis of intraocular pressure (IOP) is important for the maintenance of anterior eye anatomic integrity, minimizing pressure-associated damage to the optic nerve, and maintaining a pressure gradient for blood flow to the eye. IOP is regulated by equilibrium between aqueous humor (AH) production and its outflow. The ciliary body (CB) is thought to actively secrete AH. However, whether AH composition and in particular, its phospholipids are entirely due to CB secretion remains uncertain. Comparison of phospholipids released by cultured CB, phospholipids present within CB tissue, within AH, and within blood and serum are consistent with release of most phospholipids into the AH by the CB...
October 5, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28980723/role-of-pnca-gene-mutations-w68r-and-w68g-in-pyrazinamide-resistance
#7
Mansi Aggarwal, Aditi Singh, Sonam Grover, Bharati Pandey, Anchala Kumari, Abhinav Grover
Mycobacterium tuberculosis (Mtb) resistance towards anti-tuberculosis drugs is a widespread problem. Pyrazinamide (PZA) is a first line antitubercular drug that kills semi-dormant bacilli when converted into its activated form i.e. pyrazinoic acid (POA) by Pyrazinamidase (PZase) enzyme coded by pncA gene. In this study, we conducted several analyses on native and mutant structures (W68R, W68G) of PZase before and after docking with the PZA drug to explore the molecular mechanism behind PZA resistance caused due to pncA mutations...
October 5, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28980720/structural-insights-into-impact-of-y134f-mutation-and-discovery-of-novel-fungicidal-compounds-against-cyp51-in-puccinia-triticina
#8
Bharati Pandey, Pradeep Sharma
Sterol 14α-Demethylase Cytochrome P450 (CYP51) protein involved in ergosterol biosynthesis pathways turn out to be a crucial target for the fungicidal compound. However, the recognition mechanism and dynamic behavior of CYP51 in wheat leaf rust pathogen, Puccinia triticina, is still obscure. Previously, a mutation at position 134 (Y134F) was reported in five European isolates of P. triticina, conversely, structural basis of this mutation remains unclear. To address this problem, CYP51 protein from P. triticina was successfully built using homology modeling approach...
October 5, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28980719/mir-132-3p-regulates-adamts-5-expression-and-promotes-chondrogenic-differentiation-of-rat-mesenchymal-stem-cells
#9
Xiaozhong Zhou, Dixin Luo, Hongtao Sun, Yong Qi, Wangyang Xu, Xunjie Jin, Chao Li, Zhousheng Lin, Guitao Li
Previous study showed that miRNA aberrant expression is involved in chondrogenic differentiation. In this study, we aimed to investigate the effects of miR-132-3p on chondrogenic differentiation and the underlying mechanisms. Firstly, quantitative PCR were performed to determine the level of MiR-132-3p. Then, we used luciferase assay to examine the target of miR-132-3p. Proteoglycan was tested by Alcian blue staining assay. Moreover, the sex determining region Y-box 9 (SOX9), Collagen type II alpha 1 chain (COL2A1) and Aggrecan (ACAN) levels were analyzed by quantitative PCR, immunofluorescence and Western blotting...
October 5, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28980718/identification-of-over-expressed-proteins-in-oral-submucous-fibrosis-by-proteomic-analysis
#10
Tapasi Das, Chandraday Prodhan, Santunu Patsa, Jay Gopal Ray, Keya Chaudhuri
Early detection and identification of oral pre-malignancy or malignancy help in management of the disease and improve survival rates. Oral submucous fibrosis (OSMF) is a major threat to public health worldwide and especially in Southeast Asian countries. Identification of biomarkers is a necessary step towards early diagnosis and treatment. In this study, differentially expressed proteins between oral submucous fibrotic tissue and normal control tissues were recorded by proteomic analysis using two dimensional electrophoresis (2DE) and MALDI TOF mass spectrometry...
October 5, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28980707/microrna-588-is-upregulated-in-human-prostate-cancer-with-prognostic-and-functional-implications
#11
Nan Zhao, Tao Lin, Changbo Zhao, Shikai Zhao, Shiming Zhou, Yuqiao Li
BACKGROUND: Epigenetic factors of microRNAs (miRNAs) are important biomarkers and modulators in human prostate cancer (PCa). In this work, we characterized the expression, biomarker-potential and functional regulation of miR-588 in PCa. METHODS: Endogenous miR-588 levels were quantified by qRT-PCR in both prostate-specific antigen (PSA)-negative and PSA-positive PCa cell lines, as well as human PCa tumors. The associations between endogenous miR-588 and PCa patients' clinical outcomes and postoperative overall survival were statistically examined...
October 5, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28980703/interleukin-1-receptor-associated-kinase-1-correlates-with-metastasis-and-invasion-in-endometrial-carcinoma
#12
Yilin Wang, Yanyan Wang, Xianzhi Duan, Yinuo Wang, Zhenyu Zhang
Endometrial carcinoma (EC) is one of the most common malignancies in the world. Previous studies have investigated the altered expression of interleukin-1 receptor-associated kinase 1 (IRAK1) in various cancers. We aimed at exploring the biological function and the underlying molecular mechanism of IRAK1 in EC. In this study, IRAK1 was found elevated in EC compared with normal tissues. Further, high IRAK1 expression level was correlated with higher tumor stage, lymph node metastasis, myometrial invasion and lower survival rate...
October 5, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28941328/inducing-indel-mutation-in-the-sox6-gene-by-zinc-finger-nuclease-for-gamma-reactivation-an-approach-towards-gene-therapy-of-beta-thalassemia
#13
Mehran Modares, Laleh Shariati, Zahra Hejazi, Mansoureh Shahbazi, Mohammad Amin Tabatabaiefar, Hossein Khanahmad
β-thalassemia is a common autosomal recessive disorder characterized by a deficiency in the synthesis of β-chains.Evidences show that increased HbF levels improve the symptoms in patients with β-thalassemia or sickle cell anemia. In this study, ZFN technology was applied to induce a mutation in the binding domain region of SOX6 to reactivate γ-globin expression. The sequences coding for ZFP arrays were designed and sub cloned in TDH plus as a transfer vector. The ZFN expression was confirmed using Western blot analysis...
September 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28941308/in-vivo-and-in-vitro-effects-of-microrna-124-on-human-gastric-cancer-by-targeting-jag1-through-the-notch-signaling-pathway
#14
Hai-Juan Xiao, Qing Ji, Lin Yang, Ren-Ting Li, Cheng Zhang, Jun-Ming Hou
In this study, we aim to determine the function of miR-124 on gastric cancer (GC) cells and the underlying mechanism that involves jaddeg1 (JAG1) and the Notch signaling pathway. GC tissues and adjacent tissues from 100 patients suffering from GC were selected. GC SGC-7901 and AGS cells were selected and grouped into control, mimic-NC, miR-124 mimic, inhibitor-NC, miR-124 inhibitor, and miR-124 inhibitor + si-JAG1 groups. RT-qPCR and a western blotting assay were conducted to detect the expression of miR-124, JAG1 and Notch signaling pathway-related proteins (NICD, HES1 and HES5)...
September 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28941304/collagen-agarose-alginate-and-matrigel-hydrogels-as-cell-substrates-for-culture-of-chondrocytes-in-vitro-a-comparative-study
#15
Zhikang Miao, Zhenhui Lu, Huayu Wu, Hui Liu, Muyan Li, Danqing Lei, Li Zheng, Jinmin Zhao
Autologous chondrocyte implantation (ACI) has emerged as a new approach to cartilage repair through the use of harvested chondrocytes. But the expansion of the chondrocytes from the donor tissue in vitro is restricted by limited cell numbers and dedifferentiation of chondrocytes. In this study, we used four types of hydrogels including agarose, alginate, Matrigel and collagen type I hydrogels to serve as cell substrates and investigated the effect on proliferation and phenotype maintenance of chondrocytes. As a substrate for monolayer culture, collagen facilitated cell expansion and effectively suppressed the dedifferentiation of chondrocytes, as evidenced by fluorescein diacetate / propidium iodide (FDA/PI), hematoxylin-eosin staining (HE), Safranin O, immunofluorescenceassay, biochemistry analysis and quantitative real-time polymerase chain reaction (qRT-PCR)...
September 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28941302/shh-gene-silencing-suppresses-epithelial-mesenchymal-transition-proliferation-invasion-and-migration-of-cervical-cancer-cells-by-repressing-the-hedgehog-signaling-pathway
#16
Feng Zhang, Chen-Chen Ren, Ling Liu, Yan-Nan Chen, Li Yang, Xiao-An Zhang, Xiao-Ming Wang, Feng-Jing Yu
The study aimed to investigate the mechanism by which the sonic Hedgehog (SHH) gene silencing acts upon epithelial-mesenchymal transition (EMT), proliferation, invasion and migration of cervical cancer (CC) cells via the Hedgehog signaling pathway. RT-qPCR and western blotting were all employed to detect the SHH mRNA and protein expressions. HeLa and CasKi cells were cultured and subsequently divided into the blank, negative control (NC) and SHH-RNAi groups. A cell counting kit-8 (CCK-8) assay was utilized for cell proliferation...
September 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28941295/the-roles-of-interleukin-1-and-rhoa-signaling-pathway-in-rat-epilepsy-model-treated-with-low-frequency-electrical-stimulation
#17
Ai-Hua Liu, Ya-Ting Wu, Li-Ping Li, Yu-Ping Wang
This study aims to explore the correlation between interleukin-1 (IL-1) and epilepsy in rats when treated with low-frequency electrical stimulation via the RhoA/ROCK signaling pathway. Twenty-four SD rats were elected for this study, among which six rats were assigned as the normal group. And 16 rat models with epilepsy were successfully established and assigned into the model group, the ES group and the ES + IL-8 group, with each group comprising of six rats. The seizure frequency and duration was recorded...
September 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28941255/effects-of-placebo-controlled-insulin-sensitizing-drugs-on-hormonal-parameters-in-polycystic-ovary-syndrome-patients-a-network-meta-analysis
#18
Yong-Hui Dai, Rong Huang, Peng-Fei Zhao, Jian-Hua Xu, Dong-Dong Liu, Fu-Dong Luo
This network meta-analysis was conducted to compare effects of different placebo-controlled insulin-sensitizing drugs, including metformin, pioglitazone, rosiglitazone and troglitazone on hormonal parameters in polycystic ovary syndrome (PCOS) patients. We searched PubMed, EMBASE and Cochrane Library databases from their inception to July 2017. Randomized controlled trials (RCTs) met our inclusion criteria were included. We combined direct and indirect evidences to evaluate weighted mean difference (WMD) value and draw surface under the cumulative ranking curve (SUCRA)...
September 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28929517/neural-differentiation-of-bone-marrow-mesenchymal-stem-cells-with-human-brain-derived-neurotrophic-factor-gene-modified-in-functionalized-self-assembling-peptide-hydrogel-in-vitro
#19
Hongbin Luo, Changsheng Xu, Zhiwei Liu, Lin Yang, Yunda Hong, Guisheng Liu, Huifen Zhong, Xinyi Cai, Xuping Lin, Xiaokun Chen, Changsheng Wang, Nanwen Zhang, Weihong Xu
OBJECTIVE: To investigate the biocompatibility and differentiation of human brain-derived neurotrophic factor (hBDNF) gene-modified bone marrow mesenchymal stem cells (hBDNF-rMSCs) in a functionalized self-assembling peptide hydrogel. METHODS: hBDNF were engineered in rMSCs using adenovirus vector and the enhanced green fluorescence protein (eGFP) was used as a reporter gene. MSC-specific surface markers (CD90,CD29 and CD45)were used for identifying rMSCs. Fluorescence microscope was used to detect the transfection of rMSCs...
September 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28926145/a-crispr-reimagining-new-twists-and-turns-of-crispr-beyond-the-genome-engineering-revolution
#20
Robert J Plummer, Yi Guo, Ying Peng
Despite its explosive applications in genome engineering, CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) has been developed into a versatile tool beyond its well-known nuclease function. In this prospect article, we summarize a few exciting "off-label" applications of CRISPR including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will likely elevate CRISPR tools into yet another level of sophistication and diversity, leading to many more exciting cell biological discoveries...
September 19, 2017: Journal of Cellular Biochemistry
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