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Journal of Cellular Biochemistry

Jun Wang, Yumin Zhang, Wei Song, Tao Ma, Kunzheng Wang
This study aimed to investigate the role of miR-590-5p in chondrocyte apoptosis and autophagy in response to mechanical pressure injury in vitro, as well as to elucidate its regulatory mechanism in the pathogenesis of osteoarthritis. We applied mechanical pressure of 10 MPa to chondrocytes for 60 minutes to establish the chondrocyte model of experimentally induced mechanical injury. We then investigated the expression of miR-590-5p in the injury model and the effects of miR-590-5p dysregulation on the expression of cell apoptosis-related and autophagy-related proteins...
August 16, 2018: Journal of Cellular Biochemistry
Anu Manhas, Anjali Patel, Mohsin Y Lone, Prafulla K Jha, Prakash C Jha
In the current study, we have constructed receptor-based pharmacophore models by exploiting the Plasmodium falciparum enoyl-acyl carrier protein reductase (PfENR) structural proteome. The derived models were subjected to a series of validation procedures to list the representative hypotheses that can be used for the screening of the Drug-like Diverse Database. A set of 739 molecules was retrieved and analyzed for the adsorption, distribution, metabolism, excretion and toxicity (ADMET) and drug-likeness attributes...
August 13, 2018: Journal of Cellular Biochemistry
Huan Yan, Maria A Silva, Hong Li, Linlin Zhu, Pengyun Li, Xia Li, Xiaofang Wang, Junjun Gao, Ping Wang, Zhan Zhang
Long noncoding RNAs (lncRNAs) have been reported to be abnormally expressed in several cancers and associated with the proliferation of cancer cells. The objective of this study was to investigate the role and the underlying mechanisms of DQ786243 in ovarian cancer. In the current study, DQ786243 was specifically upregulated in ovarian cancer tissues and cell lines. Knockdown of DQ786243 inhibited the ability of cell migration, invasion, proliferation, colony formation and wound healing, whereas promoted cell apoptosis and induced G0/G1 cell cycle arresting...
August 13, 2018: Journal of Cellular Biochemistry
Ruohua Zhang, Hongmin Li, Qin Guo, Lulu Zhang, Jie Zhu, Jin Ji
Propionibacterium acnes induces inflammatory and plays a vital role in the formation of comedones through activation of inflammatory cells, keratinocytes, and sebocytes. Sirtuin6 (SIRT6), along with ADP-ribosyltransferase and deacetylase, has been proposed to mediate various biological functions, including inflammation. Nevertheless, no strong experimental evidence has been provided to support the effect of SIRT6 in treatment of inflammatory situation. Therefore, this study addressed the inhibitory effect of SIRT6 against P...
August 13, 2018: Journal of Cellular Biochemistry
Bettina Hafen, Susanne Wiesner, Katrin Schlegelmilch, Alexander Keller, Lothar Seefried, Regina Ebert, Heike Walles, Franz Jakob, Norbert Schütze
Multipotent adult stem cells/precursor cells, especially of the mesenchymal and endothelial lineage, may have great potential for bone tissue engineering. Although their potential is highly recognized, not much is known about the underlying molecular mechanisms that initiate the regeneration process, connect osteogenesis, and angiogenesis and, finally, orchestrate renewal of bone tissue. Our study addressed these questions by generating two in vitro cell culture models to examine the changes in the global gene expression patterns of endothelial precursor cells and mesenchymal stem cells after 24 hours of either humoral (conditioned medium) or direct cell-cell interaction (co-culture)...
August 13, 2018: Journal of Cellular Biochemistry
Li-Hua Cao, Feng-Min Lu, Xiao-Jie Lu, Li-Yao Zhu
OBJECTIVE: To investigate the correlation between serum protein level of insulin growth factor 1 (IGF-1) and the degree of liver fibrosis in patients with chronic hepatitis C (CHC) combined with type 2 diabetes mellitus (T2DM). METHODS: The cases are divided into four groups. Then serum levels of IFG-1, alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatitis C virus (HCV) RNA, and HCV genotypes were detected simultaneously in patients with hepatitis C, liver stiffness measurement (LSM) was measured by transient elastography, and aspartate aminotransferase platelet ratio (APRI) score was determined...
August 13, 2018: Journal of Cellular Biochemistry
S Vasanthi, J Febin Prabhu Dass
Oryza sativa is vastly affected by microbial pathogen, causing blight-related diseases, which in turn deplete the growth and productivity of rice. In this study, we analyzed four bacterial rice pathogen genomes and reported on their codon usage that might have greater implication in mutation-related research. Differential codon usage indices, such as codon adaptation index (CAI), codon bias index (CBI), effective number of codons (ENc), relative synonymous codon usage (RSCU), correspondence analysis (COA), and parity plots, were applied on coding sequences of Pseudomonas fuscovaginae, Pseudomonas syringae, Xanthomonas oryzae, and Pseudomonas avenae speices...
August 13, 2018: Journal of Cellular Biochemistry
Lin-Liang Wang, Xue-Feng Yin, Xiu-Cheng Chu, Yong-Bing Zhang, Xiao-Nan Gong
As a common cause of shoulder pain and disability, rotator cuff injury (RCI) represents a debilitating condition affecting an individual's quality of life. Although surgical repair has been shown to be somewhat effective, many patients may still suffer from reduced shoulder function. The aim of the current study was to identify a more effective mode of RCI treatment by investigating the effect of platelet-derived growth factor subunit B (PDGF-B) on tendon-bone healing after RCI repair by modifying bone marrow-derived mesenchymal stem cells (BMSCs)...
August 13, 2018: Journal of Cellular Biochemistry
Hui Zou, Runying Zou, Keke Chen, Chengguang Zhu, Xin Tian, Yalan You, Xiangling He
Burkitt lymphoma is one of the most common lymphatic system cancers with poor outcome in adult patients. p53-induced apoptosis is a critical signaling for preventing tumor development. Cyclin B/cyclin-dependent kinase 1 (CDK1) phosphorylates inhibitor of apoptosis stimulating protein of P53 (iASPP) to promote iASPP nucleus localization and its inhibitory effect on p53. However, p53 is frequently mutated in Burkitt lymphoma, which gains novel oncogenic properties. Recently, the p53 family member, p63, became an attractive gene for the therapeutic strategies for patients with cancer...
August 13, 2018: Journal of Cellular Biochemistry
Zuzanna E Matysiak-Burzyńska, Magdalena Nowakowska, Kamila Domińska, Karolina Kowalska, Elżbieta Płuciennik, Agnieszka W Piastowska-Ciesielska
In mammalian cells, angiotensin II (AngII) binds to 2 distinct high-affinity plasma membrane receptors: angiotensin receptor 1 (AT1R) and angiotensin receptor 2 (AT2R). Healthy human endometrium from women of reproductive age expresses all of the components of the renin-angiotensin system. Many studies suggest that AngII, acting via AT1R, may have a role in the development and progression of cancer, which changes the expression of angiogenic factors, AngII and AT1R are correlated with the presence of endometrial cancer (EC)...
August 13, 2018: Journal of Cellular Biochemistry
Yan-Yan Li, Chun Yang, Pingting Zhou, Shijie Zhang, Yuan Yao, Dong Li
Lung cancer is one of the most malignant cancers worldwide, and lung adenocarcinoma (LAC) remains the most common histologic subtype. However, the functional significance of RNA expression-based prognosis prediction in LAC is still unclear and needs to be further studied. By utilizing the Cox multivariate regression, we established a risk score staging system to predict the outcome of patients with LAC and subsequently identified 10 genes, including PTPRH, OGFRP1, LDHA, AL365203.1, LINC02178, AL512488.1, LINC01312, AL353746...
August 13, 2018: Journal of Cellular Biochemistry
Jamshid Tabeshpour, Mahmoud Hashemzaei, Amirhossein Sahebkar
Curcumin, the main ingredient of Curcuma longa L., has been used as a spice and as a herbal medicine with different therapeutic characteristics for centuries in Asian countries. This phytochemical has been shown to possess beneficial antiplatelet activity that has introduced it as a promising candidate for the treatment of thromboembolism, atherothrombosis, and inflammatory diseases. Platelet dysfunction under different circumstances may lead to cardiovascular disease, and curcumin has been shown to have beneficial effects on platelet dysfunction in several studies...
August 11, 2018: Journal of Cellular Biochemistry
Ru Wen, Afoma C Umeano, Derek J Essegian, Uteuliyev Yerzhan Sabitaliyevich, Kai Wang, Ammad Ahmad Farooqi
MicroRNAs (miRNAs) are short non-coding single-stranded RNAs, which play significant roles in the regulation of a myriad of biological processes. Overwhelmingly increasing high-impact research has also deepened our understanding about the central role of miRNAs in cancer development, metastatic spread, and development of resistance against various drugs. Recent studies have identified miRNAs that regulate RNA expression/processing and posttranscriptional expression of important oncogenes and tumor suppressors...
August 7, 2018: Journal of Cellular Biochemistry
Ghazala Butt, Mirna Azalea Romero, Fatima Tahir, Ammad Ahmad Farooqi
Research over the decades has sequentially and systematically provided a near-complete resolution of multifaceted and therapeutically challenging nature of cancer. Drug discovery from plants has enjoyed a renaissance in the past few years. Natural products have provided many of the lead structures, which are currently being used as templates for the design and synthesis of novel compounds with biologically enhanced properties. With the maturity and diversification of technologies, there is a growing need to design high-throughput functional assays for the evaluation of the myriad of compounds being catalogued...
August 4, 2018: Journal of Cellular Biochemistry
Liang Yan, Meng-Chao Yu, Guang-Lei Gao, Hong-Wei Liang, Xin-Yan Zhou, Zhou-Ting Zhu, Chen-Yu Zhang, Ya-Bing Wang, Xi Chen
MicroRNAs (miRNAs) play an important role in the regulation of human cancers, including breast cancer (BC). In the current study, we examined the expression pattern of the miRNA miR-125a-5p in human BC tissues, tumorigenesis of BC progression. We found that miR-125a-5p was significantly downregulated in human BC tissues. Overexpression of miR-125a-5p in a xenograft mouse model indicated that miR-125a-5p may function as a tumour suppressor during carcinogenesis. To explore the molecular mechanism by which miR-125a-5p contributes to BC progression, we predicted the target genes of miR-125a-5p and identified BC susceptibility gene 1-associated protein 1 (BAP1) as a direct target...
August 4, 2018: Journal of Cellular Biochemistry
Samira Jahangiri, Majid Jafari, Mehdi Arjomand, Faramarz Mehrnejad
The cationic antimicrobial peptide GF-17, a 17-mer-derived peptide from human cathelicidin LL-37, has a significant strength in the killing of the methicillin-resistant Staphylococcus aureus and Escherichia coli strains. Herein, we conducted a series of all-atom molecular dynamics simulations to investigate the ability of GF-17 in perturbing the model membranes of the gram-positive, S. aureus, and gram-negative, E. coli, bacteria. We also explored the contributions of the specific residues in the peptide activity...
August 4, 2018: Journal of Cellular Biochemistry
Sen Miao, Ren-Ya Zhang, Wei Wang, Hong-Bo Wang, Li-Li Meng, Li-Dong Zu, Guo-Hui Fu
Recently, dedicator of cytokinesis 2 (DOCK2) has been reportedly exhibited high mutation prevalence in the Asian colorectal cancer (CRC) cohort. However, the expression pattern of DOCK2 and its clinical significance in CRC were still unknown. To characterize the role of DOCK2, a tissue microarray (TMA) containing 481 archived paraffin-embedded CRC specimens was performed by immunohistochemistry. Among which, 54 primary CRC tissues showed high expression of DOCK2 protein, while others were negative. Moreover, DOCK2 expression was positively associated with invasion depth (P < ...
August 4, 2018: Journal of Cellular Biochemistry
Ruifeng Zhao, Jing Jin, Xinyu Sun, Kai Jin, Man Wang, Mahmoud F Ahmed, Qisheng Zuo, Yani Zhang, Zhenhua Zhao, Guohong Chen, Bichun Li
This study established a single cloned chicken embryonic fibroblast (CEF) cell line. It solves the main problem of the instability of a cultured primary cell and its impact on the experiment. In this study, CEF pass through this crisis and formed a continuous cell line after subculture. We isolated single postcrisis CEF by a mouth pipette under a convert microscope then established a single cloned cell line named CSC-1-5 which passaged continuously from 96-well plates to 60 mm culture plates. CSC has a normal chicken diploid karyotype, no tumorigenicity, and a high G2/M phase cell ratio...
August 4, 2018: Journal of Cellular Biochemistry
Ying Shen, Xin Li, Bin Zhao, Yanru Xue, Shenghang Wang, Xin Chen, Jiancheng Yang, Huanhuan Lv, Peng Shang
Iron metabolism is crucial to hepatocellular carcinoma progression and is a key determinant of prognosis. Protein-protein interactions within the iron metabolism gene network were analyzed using the European Molecular Biology Laboratory's Search Tool for Recurring Instances of Neighbouring Genes/Proteins database. We obtained 423 liver hepatocellular carcinoma gene expression profiles from the Cancer Genome Atlas database. The expression and pathway enrichment of representative iron intake genes (TFRC and DMT1), utilization genes (FTH1, FTL, HIF1A, HMOX1, SLC25A37, and SLC25A38), and efflux genes (FLVCR1 and SLC40A1) was investigated in tumor and adjacent tissues...
August 4, 2018: Journal of Cellular Biochemistry
Fuyuko Takata, Shinya Dohgu, Junichi Matsumoto, Takashi Machida, Shinya Sakaguchi, Ikuya Kimura, Atsushi Yamauchi, Yasufumi Kataoka
Oncostatin M (OSM) is a member of the interleukin (IL)-6 family cytokines. We previously demonstrated that OSM induces blood-brain barrier (BBB) impairment. However, functional characterization of IL-6 family cytokines in BBB regulation and the cytokine-related intracellular signaling pathway remain unclear. In this study, we demonstrate that among IL-6 family cytokines, including IL-6 and leukemia inhibitory factor (LIF), OSM is the most potent molecule for inducing BBB dysfunction via prolonged activation of signal transducer and activator of transcription (STAT) 3 following Janus-activated kinase (JAK) activation...
August 4, 2018: Journal of Cellular Biochemistry
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