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Journal of Cellular Biochemistry

Franciele Martini, Ana Paula Pesarico, César Augusto Brüning, Gilson Zeni, Cristina Wayne Nogueira
There is a well-known relationship between the cholinergic system and learning, memory and other common cognitive processes. The process for researching and developing new drugs has lead researchers to repurpose older ones. This study investigated the effects of ebselen on the activity of acethylcholinesterase (AChE) isoforms in vitro and in an amnesia model induced by scopolamine in Swiss mice. In vitro, ebselen at concentrations equal or higher than 10 µM inhibited the activity of cortical and hippocampal G4/AChE, but not G1/AChE isoform...
February 5, 2018: Journal of Cellular Biochemistry
Maryam Farzaneh, Masoumeh Zare, Seyedeh-Nafiseh Hassani, Hossein Baharvand
Pluripotent stem cell (PSC) lines derived from embryonated avian eggs are a convenient platform for production of various recombinant proteins and vaccines. In chicks, both embryonic stem cells (ESC) and embryonic germ cells (EGC) are considered to be pluripotent cells obtained from early blastodermal cells (stage X) and gonadal tissues (stage HH28), respectively. However, the establishment and long-term maintenance of avian PSC lines faces several challenges and differs in efficiency between chick strains...
February 2, 2018: Journal of Cellular Biochemistry
Shaofeng Yang, Linghui Li, Liguo Zhu, Chao Zhang, Zhaoyong Li, Yantao Guo, Ying Nie, Zhenhua Luo
Intervertebral disc degeneration (IDD) has been widely considered as one of the main causes for low back pain (LBP) which can cause severe impact to human health and huge economic burden to worldwide society. IDD pathogenesis can be affected by extensive degradation of extracellular matrix (ECM) and the hyperproliferation of nucleus pulposus (NP) cell. During IDD process, ECM degradation enzymes MMP and ADAMTS expression increases, while ECM synthesis-related Aggrecan and COL2A1 expression decreases. In addition, Wnt signaling pathway is reportedly involved in the process of IDD...
February 2, 2018: Journal of Cellular Biochemistry
Dong HongYu, Huang ZhongCheng, Zhang HongLian, Xiao ZhiGang, Liu Qi
BACKGROUND: RE (Radiation enteritis) has been characterized by the inflammation reaction, and in this study, we aim to explore inflammatory cytokines and underlying mechanism involved in the pathogenesis of RE. METHOD: Luciferase assay was performed to explore whether polymorphism affected the expression of let-7, and also validated let-7 directly regulated f IL-6 expression. Then Elisa was performed to study the mechanism of rs13293512 polymorphism associated with enteritis occurrence...
February 1, 2018: Journal of Cellular Biochemistry
Yuanyi Wei, Xia Zhou, Liying Ren, Chunxia Wang, Yuhao Li
Prolactinomas are the most prevalent functional pituitary adenomas that cause chronic pathological hyperprolactinemia. Prolactin is known to promote cell growth and inhibit apoptosis in cells. Paeoniflorin is the principal component of radix Paeoniae alba (the main ingredient in some traditional herbal formulas clinically used for hyperprolactinemia-associated disorders). Recent findings from intensive studies have suggested that paeoniflorin regulates cell proliferation and apoptosis in many cell lines. However, the effects of paeoniflorin in pituitary tumor cells remain unknown...
February 1, 2018: Journal of Cellular Biochemistry
Huaxiang Zhao, Xueyan Qin, Qian Zhang, Xinli Zhang, Jiuxiang Lin, Kang Ting, Feng Chen
NELL-1 is a secreted protein that was originally found to be upregulated in pathologically fusing and fused sutures in non-syndromic unilateral coronal synostosis patients. Apart from the ability of NELL-1 to promote osteogenesis in long and craniofacial bones, NELL-1 reportedly inhibits the formation of several benign and malignant tumors. We previously identified a novel transcript of Nell-1 that lacked a calcium-binding epidermal growth factor (EGF)-like domain compared with full-length Nell-1; this new transcript was named Nell-1-ΔE...
February 1, 2018: Journal of Cellular Biochemistry
Seon Goo Kim, Ji Hea Yooun, Dong Eun Kim, Eung-Seok Lee, Taeg Kyu Kwon, Shin Kim, Jong Wook Park
Resistance to anoikis and growth in anchorage-independent conditions are hallmarks of highly metastatic cancer cells. Anoikis is a type of apoptosis induced by inadequate cell/extracellular matrix (ECM) attachment and an attractive anti-cancer therapeutic strategy in cancer chemotherapeutic field. Therefore, the development of anoikis-inducing agents is useful and promising to overcome cancer. When FPDHP, a novel anoikis-inducing agent, was treated within 3h, FPDHP induced massive cell detachment in various human cancer cells, irrespective of apoptosis...
February 1, 2018: Journal of Cellular Biochemistry
Endre Soreide, Janet M Denbeigh, Eric A Lewallen, Rebekah M Samsonraj, Lawrence J Berglund, Amel Dudakovic, Simon M Cool, Lars Nordsletten, Sanjeev Kakar, Andre J van Wijnen
Tendon graft healing in bone tunnels for the fixation of intra-articular ligament reconstructions may limit clinical outcome by delaying healing. This study assesses the effects of hydrogel-mediated delivery of bone anabolic growth factors in a validated model of tendon-to-bone tunnel healing. Forty-five Wistar rats were randomly allocated into three groups (BMP2-treated, GSK126-treated, and placebo). All animals underwent a tendon to bone tunnel reconstruction. Healing was evaluated at 4 weeks by biomechanical assessment, micro-computed tomography (bone mineral density, bone volume, cross sectional area of bone tunnels), and traditional histology...
February 1, 2018: Journal of Cellular Biochemistry
Aiany C Simões-Alves, Reginaldo C Silva-Filho, Glauber Ruda F Braz, Severina Cassia A Silva, Aline Isabel da Silva, Claudia J Lagranha, Mariana P Fernandes
Recent studies have shown that exposure to fluoxetine treatment induces excessive production of ROS, and alters the antioxidant defense system in various tissues and cell types, mainly the liver. When fluoxetine is administered intraperitoneally, the drug rapidly reaches high concentrations in the liver, has potentially multiple toxic effects on energy metabolism in rat liver mitochondria. The aim of this study was to evaluate the effect of pharmacological treatment with fluoxetine during critical period for development on the mitochondrial bioenergetics and oxidative stress in liver of rat adult...
February 1, 2018: Journal of Cellular Biochemistry
Aysa Rezabakhsh, Farzaneh Fathi, Hesam Bagheri Saghaei, Hassan Malekinejad, Azadeh Montaseri, Reza Rahbarghazi, Alireza Garjani
Silibin, a flavonoid from the seeds of Silybum marianum (L.) Gaertn. (Asteraceae) has been reported to produce curative properties in diabetes. Autophagy is generated by a vast array of insults for removal of damaged proteins and organelles from the cell. Inadequate autophagy promotes endothelial cells dysfunction and delays in diabetic ulcers recovery. We hypothesized that silibinin could protect endothelial cells against high glucose-induced damage by engaging autophagic responses. HUVECs viability was evaluated by MTT assay...
February 1, 2018: Journal of Cellular Biochemistry
Ayako Ushio, Ko Eto
The RNA-binding protein RBM3, a cold shock protein whose expression is elevated under hypothermic conditions, plays an important role in cell survival; however, little is known about the mechanism underlying the mild hypothermia-mediated regulation of RBM3 expression and apoptosis. Here we show that the transcription factor NF-κB p65 is phosphorylated at Ser276 and activates RBM3 gene transcription via binding to a particular element within the promoter region in response to induced hypothermia, elevating the protein expression and suppressing apoptosis...
February 1, 2018: Journal of Cellular Biochemistry
Purushoth Ethiraj, Jessica R Link, James M Sinkway, Gabriella D Brown, William A Parler, Sakamuri V Reddy
Microgravity (µXg) experienced by astronauts during space flights causes accelerated bone loss. However, the molecular basis of µXg induced bone loss in space is unclear. Osteoclast (OCL) is the primary bone-resorbing cell. We previously demonstrated that simulated µXg promotes OCL formation. In this study, we identified that µXg induces syncytin-A expression in RAW264.7 preosteoclast cells without RANKL stimulation. We further tested the effect of osteotropic factors such as CXCL5 and 1,25(OH)2 D3 to regulate the syncytin-A expression in preosteoclast cells subjected to µXg compared to ground based (Xg) cultures...
February 1, 2018: Journal of Cellular Biochemistry
Won Jin Lee, Sun Ah Ham, Hyunjin Yoo, Jung Seok Hwang, Taesik Yoo, Kyung Shin Paek, Dae-Seog Lim, Sung Gu Han, Chi-Ho Lee, Kwonho Hong, Han Geuk Seo
Neuroinflammation-associated release of glutamate from activated microglia has been implicated in the progression of neurodegenerative diseases. However, the regulatory mechanisms underlying this glutamate release are poorly understood. Here, we show that peroxisome proliferator-activated receptor delta (PPARδ) modulates neurotoxicity by inhibiting glutamate release in lipopolysaccharide (LPS)-activated BV-2 microglial cells. Activation of PPARδ by GW501516, a specific PPARδ agonist, inhibited glutamate release in BV-2 cells...
February 1, 2018: Journal of Cellular Biochemistry
Prajna Hegde, Sindhura B R, Bale M Swamy, Shashikala R Inamdar
The correlation between colorectal cancer (CRC) progression and altered expression of N-glycans can be considered in search for new biomarkers and anticancer agents to control CRC. Earlier N-glycan specific mitogenic lectin from Rhizoctonia bataticola (RBL) has been reported which has growth inhibitory and apoptotic effect on human ovarian and leukemic cells, but mitogenic effect on normal PBMCs revealing its clinical potential. Here we report the effect of RBL on human colon cancer HT 29, SW480 and SW620 cell growth and its differential binding to human normal colon and cancer tissues...
January 31, 2018: Journal of Cellular Biochemistry
Chao Yang, Ke Wu, Shan Wang, Guanghui Wei
The lncRNA XIST (X inactive-specific transcript) is an oncogenic lncRNA that is present in various malignant tumors; however, its role and molecular mechanisms in osteosarcoma (OS) progression remain unclear. In the current study, 40 pairs of OS tissues and matched adjacent non-tumor tissues were collected. qRT-PCR was conducted to investigate the differences in XIST expression in tissues and OS cell lines. The proliferation, invasion, and EMT status of OS cells after transfection were assessed with WST-1 assays, Transwell assays and western blot analysis, respectively...
January 31, 2018: Journal of Cellular Biochemistry
Xiang Gao, Han Han
Jolkinolide B (JB), a bioactive compound isolated from herbal medicine, has been found to inhibit tumor growth by altering glycolysis. However, whether glycolysis is influenced by JB in non-small cell lung cancer (NSCLC) cells and the mechanism remain unknown. The aim of the present study was to evaluate the effect of JB on the glycolysis in NSCLC cells and the underlying molecular mechanism. The results showed that JB treatment inhibited cell viability of A549 and H1299 cells in a concentration-dependent manner...
January 31, 2018: Journal of Cellular Biochemistry
Hulya Celik, Tuba Aydin, Kubra Solak, Sumbul Khalid, Ammad Ahmad Farooqi
Curcumin, a bioactive and pharmacologically efficient component isolated from Curcuma longa has attracted considerable attention because of its ability to modulate diverse cellular and physiological pathways. WNT, TGF/SMAD, NOTCH and SHH are fundamentally different signaling cascades, but their choreographed activation is strongly associated with cancer development and progression. In this review we have attempted to set spotlight on regulation of different cell signaling pathways by curcumin in different cancers...
January 31, 2018: Journal of Cellular Biochemistry
Jin-Hua Zhang, Jiang-Kun Li, Li-Li Ma, Ji-Yu Lou
OBJECTIVE: Ischemic stroke is the leading cause of worldwide mortality and long-term disability in adults. This study aims to explore the effects of RNA interference (RNAi)-mediated silencing of the S100B gene on nerve function recovery and morphological changes of hippocampus cells in rat models with ischemic stroke. METHODS: Sixty Wistar rats were assigned into different group. S100B and Caspase 3 mRNA and protein expressions were evaluated by RT-qPCR and Western blotting...
January 31, 2018: Journal of Cellular Biochemistry
Mark C Turner, Darren J Player, Neil R W Martin, Elizabeth C Akam, Mark P Lewis
Skeletal muscle is an insulin sensitive tissue and accounts for approximately 80% of post-prandial glucose disposal. This study describes the effects of insulin, delivered for 72 hours, to skeletal muscle myoblasts during differentiation or to skeletal muscle myotubes. After chronic treatment, cultures were acutely stimulated with insulin and analysed for total and phosphorylated Akt (Ser473 ), mRNA expression of metabolic and myogenic markers and insulin-stimulated glucose uptake. Skeletal muscle cells differentiated in the presence of insulin chronically, reduced acute insulin stimulated phosphorylation of Akt Ser473 ...
January 31, 2018: Journal of Cellular Biochemistry
Xiaofang Zhao, Guosong Luo, Ying Cheng, Wenjing Yu, Run Chen, Bin Xiao, Yuancai Xiang, Chunhong Feng, Wenguang Fu, Chunyan Duan, Fuli Yao, Xianming Xia, Qinghua Tao, Mei Wei, Rongyang Dai
Compound C, a well-known inhibitor of AMP-activated protein kinase (AMPK), has been reported to exert antitumor activities in some types of cells. Whether compound C can exert antitumor effects in human cholangiocarcinoma (CCA) remains unknown. Here, we demonstrated that compound C is a potent inducer of cell death and autophagy in human CCA cells. Autophagy inhibitors increased the cytotoxicity of compound C towards human CCA cells, as confirmed by increased LDH release and PARP cleavage. It is notable that compound C treatment increased phosphorylated Akt, sustained high levels of phosphorylated p70S6K and decreased mTOR regulated p-ULK1 (ser757)...
January 31, 2018: Journal of Cellular Biochemistry
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