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Véréna Poinsot, Varravaddheay Ong-Meang, Audrey Ric, Pierre Gavard, Lucie Perquis, François Couderc
In the tenth edition of this article focused on recent advances in amino acid analysis using capillary electrophoresis, we describe the most important research articles published on this topic during the period from June 2015 to May 2017. This article follows the format of the previous articles published in Electrophoresis. The new developments in amino acid analysis with CE mainly describe improvements in CE associated with mass spectrometry. Focusing on applications, we mostly describe clinical works, although metabolomics studies are also very important...
August 14, 2017: Electrophoresis
Zhi-Jun Xia, Zhen Liu, Fan-Zhi Kong, Liu-Yin Fan, Hua Xiao, Cheng-Xi Cao
Antimicrobial peptides (AMPs) are usually small and cationic biomolecules with broad-spectrum antimicrobial activities against pathogens. Purifying them from complex samples is essential to study their physiochemical properties. In this work, free-flow zone electrophoresis (FFZE) was utilized to purify antimicrobial peptides from yeast fermentation broth. Meanwhile, gel filtration chromatography (GFC) was conducted for comparison. The separation efficiency was evaluated by SDS-PAGE analysis of the fractions from both methods...
August 12, 2017: Electrophoresis
Jiri Moravec, Jan Mares
We introduce an optimized periodic acid - Schiff´s staining of glycoproteins on 1D electrophoretic gels. Thanks to heating in a household microwave oven the protocol of standard periodic acid - Schiff´s staining has been accelerated from 6 h to below 10 min employing standard chemistry. At the same time, we show that the microwave-assisted glycoprotein staining is at least as sensitive as the conventional approach. This article is protected by copyright. All rights reserved.
August 12, 2017: Electrophoresis
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August 12, 2017: Electrophoresis
Yuanyuan Liu, Weiwei Wang, Mengqi Jia, Rangdong Liu, Qing Liu, Han Xiao, Jing Li, Yun Xue, Yan Wang, Chao Yan
This review focuses on the recent advances on microscale separation technology, particularly on the new development on column and detection technologies in electrophoretic and chromatographic separation modes (i.e., CE, CEC, cLC, etc.). Representative examples illustrate also applications on the small molecules, peptides, proteins, polysaccharides, chiral and so on. 247 relevant articles are cited covering the literature published from 2013 to May, 2017. This article is protected by copyright. All rights reserved...
August 12, 2017: Electrophoresis
Thaís Cidália Vieira, Marc Alexandre Duarte Gigonzac, Ricardo Goulart Rodovalho, Luana Morais Cavalcanti, Lysa Bernardes Minasi, Flávia Melo Rodrigues, Aparecido Divino da Cruz
The appearance of new mutations in polymorphic markers plays a central role in a range of genetic applications, including dating phylogenetic events, informing disease studies, and evaluating forensic evidence. The present study estimated the mutation rates of 21 autosomal short tandem repeat (STR) loci in a population from Central Brazil. We studied 15,046 paternity cases from Goiás, Brazil from August 2012 to February 2015. We identified 262 mutations in the 21 loci. The loci that presented more mutations were FGA and D18S51, with a total of 46 and 28 mutations, respectively...
August 9, 2017: Electrophoresis
Monica Di Venere, Simona Viglio, Maddalena Cagnone, Anna Bardoni, Roberta Salvini, Paolo Iadarola
Aim of this article is to focus the attention of the reader on the application of CE/MS and LC/MS to the analysis of human body fluids not currently used for the diagnosis of disorders and, for this reason, catalogued as "less-/non-conventional" fluids, i.e. tears, nasal secretions, cerumen, bronchoalveolar lavage fluid, sputum, exhaled breath condensate, nipple aspirate, breast milk, amniotic fluid, bile, seminal plasma, liposuction aspirate fluid and synovial fluid. The pool of articles presented in this report demonstrates that, rather than being neglected, these fluids are an important resource for the evaluation of possible pathologic conditions...
August 9, 2017: Electrophoresis
María Ramos-Payán, Juan A Ocaña-Gonzalez, Rut M Fernández-Torres, Andreu Llobera, Miguel Ángel Bello-López
Capillary electrophoresis has been a continuously evolving analytical methodology since its first introduction in the 80s of the last century. The development of new CE separation procedures, the coupling of these systems to more sensitive and versatile detection systems and the advances in miniaturization technology have allowed the application of CE to the resolution of new and complex analytical problems, overcoming the traditional disadvantages associated with this method. In the present work, different recent trends in capillary electrophoresis and their application to the determination of high complexity samples (as biological fluids, individual cells, etc...
August 8, 2017: Electrophoresis
Süleyman Aşir, Duygu Sari, Ali Derazshamshir, Fatma Yılmaz, Koray Şarkaya, Adil Denizli
A dopamine imprinted monolithic column was prepared and used in capillary electrochromatography as stationary phase for the first time. Dopamine was selectively separated from aqueous solution containing the competitor molecule norepinephrine, which is similar in size and shape to the template molecule. Morphology of the dopamine imprinted column was observed by scanning electron microscopy. The influence of the organic solvent content of mobile phase, applied pressure and pH of the mobile phase on the recognition of dopamine by the imprinted monolithic column has been evaluated, and the imprinting effect in the dopamine-imprinted monolithic polymer was verified...
August 8, 2017: Electrophoresis
Ederson Paulo Xavier Guilherme, Jocilane Pereira de Oliveira, Lorendane Millena de Carvalho, Igor Viana Brandi, Sérgio Henrique Sousa Santos, Gleidson Giordano Pinto de Carvalho, Junio Cota, Bruna Mara Aparecida de Carvalho
A bioreactor was built by means of immobilizing alpha-amylase from Aspergillus oryzae by encapsulation, through cryopolymerization of acrylamide monomers for the continuous starch hydrolysis. The starch hydrolysis was evaluated regarding pH, the concentration of immobilized amylase on cryogel, the concentration of starch solution and temperature. The maximum value for starch hydrolysis was achieved at pH 5.0, concentration of immobilized enzyme 111.44mg amylase /gcriogel , concentration of starch solution 45 g/L and temperature of 35°C...
August 4, 2017: Electrophoresis
Murthy Jonnada, Ziad El Rassi
This investigation was aimed at introducing a monolithic precursor that can be conveniently grafted with the desired chromatographic ligand via the process of post polymerization modification (PPM). The precursor was obtained by the in-situ polymerization of N-acryloxysuccinimide (NAS) and ethylene glycol dimethacrylate (EDMA) in a narrow bore stainless steel column of 1 mm i.d. yielding a poly(NAS-co-EDMA) monolithic column designated as the NASM column. In a first PPM, the NASM column was bonded with octadecyl (OD) ligands yielding a nonpolar NASM-OD column that proved useful for RPC of proteins in gradient elution at increasing %ACN in the mobile phase...
August 4, 2017: Electrophoresis
Vignesh Rajamanickam, Maximillian Krippl, Christoph Herwig, Oliver Spadiut
Downstream process development for recombinant glycoproteins from yeast is cumbersome due to hyperglycosylation of target proteins. In a previous study, we purified three recombinant glycoproteins from Pichia pastoris using a simple two-step flowthrough mode approach using monolithic columns. In this study, we investigated a novel automated data science approach for identifying purification conditions for such glycoproteins using monolithic columns. We performed three sets of design of experiments (DoEs) in analytical scale to determine the separation efficiency of monolithic columns for three different recombinant horseradish peroxidase (HRP) isoenzymes...
August 4, 2017: Electrophoresis
Qianru Wang, Naga Neehar Dingari, Cullen R Buie
Insulator-based dielectrophoresis (iDEP) has emerged as a powerful tool for multiple biomicrofluidic operations, such as cell separation and concentration. The key feature for iDEP systems is the alteration of insulating microchannel geometries to create strong electric field gradients. Under AC electric fields, this strong electric field gradient can affect fluid flow by (at least) two nonlinear electrokinetic phenomena; a) electrothermal flow due to Joule heating and b) induced charge electroosmosis (ICEO) near the microchannel constrictions of small (but finite) permittivity and conductivity...
August 1, 2017: Electrophoresis
Sandya R Beeram, Elliott Rodriguez, Suresh Doddavenkatanna, Zhao Li, Allegra Pekarek, Darin Peev, Kathryn Goerl, Gianfranco Trovato, Tino Hofmann, David S Hage
The development of various nanomaterials over the last few decades has led to many applications for these materials in liquid chromatography (LC). This review will look at the types of nanomaterials that have been incorporated into LC systems and the applications that have been explored for such systems. A number of carbon-based nanomaterials and inorganic nanomaterials have been considered for use in LC, ranging from carbon nanotubes, fullerenes and nanodiamonds to metal nanoparticles and nanostructures based on silica, alumina, zirconia and titanium dioxide...
August 1, 2017: Electrophoresis
Ashish Khaparde, Vijayalakshmi M A, Kali Kishore Reddy Tetala
Herein, we report the preparation of a metal-chelate immobilized hydrophilic poly hydroxyethylmethacrylate based monolith of 100 μL volume in a plastic syringe. The monolith is elastic in nature, contains well interconnected pores with a permeability (k) of 1.3 × 10(-12)  m(2) . Immobilization of iminodiacetic acid (IDA) is performed via schiff base reaction. Adsortion of IgG on this copper-IDA monolith is of langmuir isotherm with a maximum adsorption capacity of ∼25 mg IgG per g monolith. IgG adsorption capacity of this affinity monolith remained unaffected with increase of flow rate...
August 1, 2017: Electrophoresis
Alain Wuethrich, Joselito P Quirino
Derivatisation is an integrated part of many analytical workflows to enable separation and detection of the analytes. In CE, derivatisation is adapted in the four modes of pre-capillary, in-line, in-capillary, and post-capillary derivatisation. In this review, we discuss the progress in derivatisation from February 2015 to May 2017 from multiple points of view including sections about the derivatisation modes, derivatisation to improve the analyte separation and analyte detection. The advancements in derivatisation procedures, novel reagents, and applications are covered...
July 31, 2017: Electrophoresis
Tamara Martinović, Djuro Josić
The experiences in the production and application of polymethacrylate-based monolithic supports, since their development almost thirty years ago, are presented. The main driving force for the development of new chromatographic supports was the necessity for isolation and separation of physiologically active biopolymers and their use for therapeutic purposes. For this sake, a development of a method for fast separation, preventing denaturation and preserving their biological activity was necessary. Development of polysaccharide-based supports, followed by the introduction of polymer-based chromatographic media, is shortly described...
July 31, 2017: Electrophoresis
Mario Braun, Wolfgang Gebauer, Gabriela Krczal, Christiane Ziegler, Christine Müller-Renno, Kajohn Boonrod
We present a simple method to estimate the isoelectric point (pI) of Tomato Bushy Stunt (TBSV) particles. We demonstrate that the combination of agarose gels with different pH buffers can be used to electrophorese the virus particles and their migration patterns can be compared. This method allows to estimate the pI of the virus particles (wild type, wt) and genetically modified particles) and to monitor the effect of the pI of modified peptide side-chains of the viral capsid subunit on the pI of the whole virus particle...
July 31, 2017: Electrophoresis
Aleksei Kabedev, Mark Ross-Lonergan, Vladimir Lobaskin
We study solute transport in a microfluidic channel, where the walls hold an array of tilted rigid nanopillars. By solving numerically the flow equations in the channel, we show that a combination of hydrodynamic effects with excluded volume interactions between the solute particles and the pillars leads to a hydrodynamic lift effect, which varies with the particle size, and depends in a strongly nonlinear fashion on the flow rate. We show that the lift force can be sufficiently strong to drive the solute accumulation or removal from the pillar region and can be switched to the opposite direction by variation of the shear rate or driving pressure...
July 28, 2017: Electrophoresis
Seyed Ammar Haeri, Shahryar Abbasi, Sami Sajjadifar
In this paper, biosorption based dispersive liquid-liquid microextraction in combination with magnetic solid phase extraction has been developed as a sample pretreatment method with high enrichment factor for the sensitive determination of ibuprofen in water samples. At first, magnetic Fe3 O4 /polypyrrole nanoparticles were synthesized and employed as sorbent for the magnetic solid phase extraction of ibuprofen. After the elution of the desired compound from the sorbent by using methanol, bio-sorption based dispersive liquid-liquid microextraction technique was performed on the obtained solution...
July 28, 2017: Electrophoresis
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