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Marcela Mireles Ramirez, Thomas R Gaborski
The fabrication of nanostructured materials is an area of continuous improvement and innovative techniques that fulfill the demand of many fields of research and development. The continuously decreasing size of the smallest patternable feature has expanded the catalog of methods enabling the fabrication of nanostructured materials. Several of these nanofabrication techniques have sprouted from applications requiring nanoporous membranes such as molecular separations, cell culture, and plasmonics. This review summarizes methods that successfully produce through-pores in ultrathin films exhibiting an approximate pore size to thickness ratio of one, which has been shown to be beneficial due to high permeability and improved separation potential...
May 19, 2017: Electrophoresis
Tan Xue Ting, Fairuz Binti Amran, Ravindran Thayan, Norazah Ahmad, Roslinda Jaafar, Rahimah Haron, Rafidah Abdullah, Sazwan Reezal Bin Shamsuddin, Nor Suhaila Binti Md Riffin, Puteri Shafinaz Abdul-Rahman
Leptospirosis is an emerging zoonotic infectious disease in Malaysia. The symptoms of leptospirosis vary from mild non-specific flu-like illness to a severe condition which is usually associated with serious complication and fatality. To study the protein expression profile of mild and severe leptospirosis, fifteen paired sera were collected from the patients who were mildly infected and following that progressed to severe stage. The proteome profiles of mild and severe cases were studied using 2DE analysis in combination with LC-MS/MS...
May 19, 2017: Electrophoresis
Wenjing Peng, Yu Zhang, Rui Zhu, Yehia Mechref
Breast cancer is the leading type of cancer in women. Breast cancer brain metastasis is currently considered an issue of concern among breast cancer patients. Membrane proteins play important roles in breast cancer brain metastasis, involving cell adhesion and penetration of blood-brain barrier. To understand the mechanism of breast cancer brain metastasis, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed in conjunction with enrichment of membrane proteins to analyze the proteomes from 5 different breast cancer and a brain cancer cell lines...
May 19, 2017: Electrophoresis
Juan Carlos García-Cañaveras, Manuel David Peris-Díaz, M Isabel Alcoriza, Manuela Cerdán-Calero, M Teresa Donato, Agustín Lahoz
Phospholipidosis and steatosis are two toxic effects, which course with overaccumulation of different classes of lipids in the liver. MS-based lipidomics has become a powerful tool for the comprehensive determination of lipids. LC-MS lipid profiling of HepG2 cells is proposed as an in vitro assay to study and anticipate phospholipidosis and steatosis. Cells with and without pre-incubation with a mixture of free fatty acids (FFA) (i.e., oleic and palmitic) were exposed to a set of well-known steatogenic and phospholipidogenic compounds...
May 17, 2017: Electrophoresis
Claudia Spahn, Michael Wermann, Rico Eichentopf, Gerd Hause, Dagmar Schlenzig, Stephan Schilling
Recombinant expression and purification of amyloid peptides represents a common basis for investigating the molecular mechanisms of amyloid formation and toxicity. However, the isolation of the recombinant peptides is hampered by inefficient separation from contaminants such as the fusion protein required for efficient expression in E. coli. Here, we present a new approach for the isolation of highly purified Aβ(1-42) and pGlu-Aβ(3-42), which is based on a separation using preparative SDS-PAGE. The method relies on the purification of the Aβ fusion protein by affinity chromatography followed by preparative SDS-PAGE under reducing conditions and subsequent removal of detergents by precipitation...
May 16, 2017: Electrophoresis
Rachel B Strickhouser, Nahla A Hatab, Nickolay V Lavrik, Michael J Sepaniak
A fundamental problem with efficiency in capillary action driven planar chromatography results from diminishing flow rates as development proceeds, giving rise to molecular diffusion related band dispersion for most sample types. Overpressure and electrokinetic means to speed flow have been used successfully in TLC. We explore the use of centrifugal force (CF) to drive flow for reduced-dimension planar platforms (ultra-TLC, low μm features, and nano-TLC, nanoscale features). The silicon wafer platforms have two forms of continuous 2-D arrays created by either photolithography or metal dewetting followed by deep reactive ion etching and coated with porous SiO2 ...
May 15, 2017: Electrophoresis
Rosa Letizia Zaffino, Mònica Mir, Josep Samitier
Nano-gap electrodes have attracted a lot of consideration as promising platform for molecular electronic and biomolecules detection. This is mainly for their higher aspect ratio, and because their electrical properties are easily accessed by current-voltage measurements. Nevertheless, application of standard current-voltages measurements used to characterize nano-gap response, and/or to modify specific nano-gap electrodes properties, represents an issue. Since the strength of electrical fields in nano-scaled devices can reach high-values, even at low voltages...
May 15, 2017: Electrophoresis
Lina Zhou, Peiyuan Yin, Ping Luo, Ling Tang, Zhichao Wang, Peng Gao, Hailong Piao, Xin Lu, Guowang Xu
Common metabolomics platforms require about 10(6) cells, which has a limited throughput due to the time-consuming steps of cell culture and preparation. There is a demand for metabolic profiling methods to improve analytical throughput and detection sensitivity based on small amount of cells. In this study, we proposed a high-throughput scheme, integrating 96-well plate cell cultivation, in-situ cell pretreatment and sensitive dansylation labelling coupled with liquid chromatography-mass spectrometry analysis of metabolites inside hepG2 cells (of the order of magnitude of 10(3) cells in each well)...
May 12, 2017: Electrophoresis
Xin Guo, Kun Wang, Guan-Hua Chen, Jie Shi, Xian Wu, Lu-Lu Di, Yi Wang
A nonaqueous micellar electrokinetic capillary chromatography method with indirect laser induced fluorescence was developed for the determination of strobilurin fungicide residues in fruits and vegetables. Hydrophobic CdTe quantum dots (QDs) synthesized in aqueous phase were used as background fluorescent substance. The background electrolyte (BGE) solution, QD concentration and separation voltage were optimized to obtain the best separation efficiency and the highest signal intensity. The optimal BGE solution consists of 40 mM phosphate, 120 mM sodium dodecyl sulfate, 15% (V/V) water and 15% (V/V) hydrophobic CdTe QDs in formamide, of which apparent pH is 9...
May 12, 2017: Electrophoresis
Mladen Krajacic, Maja Ravnikar, Aleš Štrancar, Ion Gutiérrez-Aguirre
Key properties of monolithic chromatographic supports, make them suitable for separation and/or concentration of large biomolecules, especially virus particles and viral genomes. One by one, the studies that have been completed so far, contributed to the knowledge that monolith chromatography has hardly any limitation to be applied in virus research. Viruses of different sizes, possessing icosahedral structure and symmetrical morphology, as well as rod-shaped or filamentous viruses with helical structure, even enveloped ones, all of them could be successfully managed by means of monolith chromatography...
May 12, 2017: Electrophoresis
Tereza Tůmová, Lenka Monincová, Ondřej Nešuta, Václav Čeřovský, Václav Kašička
Capillary ITP (CITP) and CZE were applied to the determination of effective charges and ionic mobilities of polycationic antimicrobial peptides (AMPs). Twelve AMPs (deca- to hexadecapeptides) containing three to seven basic amino acid residues (His, Lys, Arg) at variable positions of peptide chain were investigated. Effective charges of the AMPs were determined from the lengths of their ITP zones, ionic mobilities, and molar concentrations, and from the same parameters of the reference compounds. Lengths of the ITP zones of AMPs and reference compounds were obtained from their CITP analyses in cationic mode using leading electrolyte (LE) composed of 10 mM NH4 OH, 40 mM AcOH (acetic acid), pH 4...
May 11, 2017: Electrophoresis
Sachin Pangavhane, Stanislav Böhm, Emanuel Makrlík, Paolo Ruzza, Václav Kašička
ACE and density functional theory (DFT) were employed to study the noncovalent interactions of cyclic decapeptide glycine-6-antamanide ([Gly(6) ]AA), synthetic derivative of native antamanide peptide from the deadly poisonous fungus Amanita phaloides, with small cations (Li(+) , Rb(+) , Cs(+) , NH4(+) , and Ca(2+) ) in methanol. The strength of these interactions was quantified by the apparent stability constants of the appropriate complexes determined by ACE. The stability constants were calculated using the nonlinear regression analysis of the dependence of the effective electrophoretic mobility of [Gly(6) ]AA on the concentration of the above ions in the BGE (methanolic solution of 20 mM chloroacetic acid, 10 mM Tris, pHMeOH 7...
May 9, 2017: Electrophoresis
Mauro Sérgio Ferreira Santos, Fernando Silva Lopes, Ivano Gebhardt Rolf Gutz
An EC-CE-C(4) D flow system was applied to the investigation of electrocatalytic processes by monitoring carboxylic acids formed during the electro-oxidation at various potentials of primary alcohols (mixture of 1 mmol L(-1) of ethanol, n-propanol, n-butanol and n-pentanol) in acidic, neutral and alkaline media. The electro-oxidation was carried out on gold and platinum disk electrodes (3 mm of diameter) in a thin-layer electrochemical flow cell. Products were sampled 50 μm apart from the electrode directly into the capillary...
May 9, 2017: Electrophoresis
María Encarnación Blanco, Oskar González, Oihane Elena Albóniga, M Luz Alonso, Rosa M Alonso
A pilot study for the investigation of the maturation grade of children has been carried out using plasma samples already analyzed in a previous pharmacokinetic study. By using a meticulous data treatment, possible confounding factors that may hinder the obtained results were identified. By doing so, it was possible to obtain enough evidence to support the feasibility of performing a larger study eluding some unwanted variability and minimizing not only the number of subjects involved but also the time and money spent on the study...
May 8, 2017: Electrophoresis
David Sýkora, Jiří Vozka, Eva Tesařová, Květa Kalíková, Martin Havlík, Pavel Matějka, Vladimír Král
A new ion-exchanger type chiral stationary phase for high-performance liquid chromatography was prepared. The synthetic protocol is based on derivatization of silica with (3-iodopropyl)trimethoxysilane in the first step followed by immobilization of strychnine via quaternization of nitrogen atom of the alkaloid strychnine. The synthesized chiral stationary phase was chromatographically characterized. The main effort was headed towards the evaluation of the enantioselectivity of the novel sorbent. For that purpose a set of suitable chiral probes, specifically, binaphthyl derivatives, was employed...
May 5, 2017: Electrophoresis
Zhao Li, Elliott Rodriguez, Shiden Azaria, Allegra Pekarek, David S Hage
Affinity monolith chromatography, or AMC, is a liquid chromatographic method in which the support is a monolith and the stationary phase is a biological-binding agent or related mimic. AMC has become popular for the isolation of biochemicals, for the measurement of various analytes, and for studying biological interactions. This review will examine the principles and applications of AMC. The materials that have been used to prepare AMC columns will be discussed, which have included various organic polymers, silica, agarose, and cryogels...
May 5, 2017: Electrophoresis
Fumihiko Kitagawa, Tatsuya Ishiguro, Misaki Tateyama, Isoshi Nukatsuka, Kenji Sueyoshi, Takayuki Kawai, Koji Otsuka
A combination of two online sample concentration techniques, large-volume sample stacking with an electroosmotic flow (EOF) pump (LVSEP) and field-amplified sample injection (FASI), was investigated in microchip electrophoresis (MCE) to achieve highly sensitive analysis. By applying reversed-polarity voltages on a cross-channel microchip, anionic analytes injected throughout a microchannel were first concentrated on the basis of LVSEP, followed by the electrokinetic stacking injection of the analytes from a sample reservoir by the FASI mechanism...
May 5, 2017: Electrophoresis
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No abstract text is available yet for this article.
May 3, 2017: Electrophoresis
Jordon Gilmore, Monsur Islam, Josie Duncan, Rucha Natu, Rodrigo Martinez-Duarte
No abstract text is available yet for this article.
May 3, 2017: Electrophoresis
Raúl González-Domínguez, Rosa María Mateos, Alfonso María Lechuga-Sancho, José Joaquín González-Cortés, Manuel Corrales-Cuevas, Juan Alberto Rojas-Cots, Carmen Segundo, Mónica Schwarz
High sugar consumption elicits numerous deleterious effects on health by inducing insulin resistance, which is closely associated with the development of metabolic disorders such as obesity or type-2 diabetes. Furthermore, there is also growing evidence that caffeine may play an important role in the regulation of insulin release and the appearance of related metabolic impairments. Thus, the aim of this work was to investigate the impact of acute sugar and caffeine intake on the metabolic health status by using a metabolomic multi-platform based on the combination of flow injection mass spectrometry and ultra-high performance liquid chromatography mass spectrometry...
May 3, 2017: Electrophoresis
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