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Fangfang Gao, Wenhui Lu, Huitao Liu, Jinhua Li, Lingxin Chen
Dispersive liquid-liquid microextraction (DLLME) coupled with capillary electrophoresis (CE) was developed for simultaneous determination of five types of chlorophenols (CPs), namely 2-chlorophenol (2-CP), 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP), 2,6-dichlorophenol (2,6-DCP) and 2,4,6-trichlorophenol (2,4,6-TCP) in water samples. Several parameters affecting DLLME and CE conditions were systematically investigated. Under the optimized DLLME-CE conditions, the five CPs were separated completely within 7...
July 13, 2018: Electrophoresis
Chia-Chun Hsu, Yan-Chang Lee, Wen-Hsin Hsieh
This study uses negative dielectrophoresis and AC electroosmosis as a driving mechanism and presents an electrically driven microconcentrator that concentrates the sample in the region exterior to the electrodes (termed as exterior-electrode electrically driven microconcentrator in this paper). The proposed microconcentrator uses a 3-D face-to-face electrode pair; the top electrode is a relatively large planar electrode, and the bottom electrode is formed with three to six long and thin electrodes connected into an open ring...
July 13, 2018: Electrophoresis
Zhijian Liu, Di Li, Maryam Saffarian, Tzuen-Rong Tzeng, Yongxin Song, Xinxiang Pan, Xiangchun Xuan
Previous studies have reported a lateral migration in particle electrophoresis through a straight rectangular microchannel. This phenomenon arises from the inherent wall-induced electrical lift that can be exploited to focus and separate particles for microfluidic applications. Such a dielectrophoretic-like force has been recently found to vary with the buffer concentration. We demonstrate in this work that the particle zeta potential also has a significant effect on the wall-induced electrical lift. We perform an experimental study of the lateral migration of equal-sized polystyrene particles with varying surface charges under identical electrokinetic flow conditions...
July 13, 2018: Electrophoresis
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July 12, 2018: Electrophoresis
Mohammadhassan Foroughbakhshfasaei, Zoltán-István Szabó, Arash Mirzahosseini Péter Horváth, Gergő Tóth
A novel, fast and economic chiral HPLC method was developed and validated for the resolution of the four isomers of tofisopam. The separation capacity of eleven different chiral columns: six polysaccharide-type including three amylose-based (Chiralpak AD, Chiralpak AD-RH and Chiralpak AS) and three cellulose-based (Chiralcel OD, Chiralcel OJ and Lux Cellulose-4); three cyclodextrin- (Quest-BC, Quest-C2 and Quest-CM) and two macrocyclic glycopeptide antibiotic-type (Chirobiotic T and Chirobiotic TAG) were screened using polar organic or reversed-phase mode...
July 12, 2018: Electrophoresis
Enrique Javier Carrasco-Correa, Martina Ferri, Ulrich Woiwode, Yubo Ma, José Manuel Herrero-Martínez, Guillermo Ramis-Ramos, Wolfgang Lindner, Michael Lämmerhofer
Thiol-ene click reaction of N-acetyl-L-cysteine methyl ester to codeine, followed by reaction with allyl isocyanate and hydrolysis to the corresponding zwitterionic chiral selector, which was bonded to the surface of a methacrylate monolith. First, the epoxy groups were converted into amine residues, followed by reaction with allylglycidyl ether. In this way, a spacer arm was bonded to the surface before coating and cross-linking poly(3-mercaptopropyl methylsiloxane) (PMPMS) via radical addition (thiol-ene click reaction) to the surface...
July 12, 2018: Electrophoresis
Xiaohan Chen, Liang Wang, Golbarg M Roozbahani, Youwen Zhang, Jialing Xiang, Xiyun Guan
Baxα, a key tumor suppressor gene, will not be expressed correctly as a result of single nucleotide mutation in its microsatellite region; Instead, BaxΔ2, an isoform of Baxα, is often produced. In addition to lack of the exon 2 due to an alternative splicing, BaxΔ2 has the same sequence as Baxα except single base deletion from eight continuous guanines (G8) to G7. Most of the currently available methods for Bax∆2 detection are inefficient and time-consuming, and/or require the use of labels or dyes. In this work, we reported a label-free nanopore sensing strategy to differentiate between Baxα and BaxΔ2 with a DNA polymer as a molecular probe based on alternative spliced sequences...
July 12, 2018: Electrophoresis
Paweł Mateusz Nowak, Katarzyna Olesek, Michał Woźniakiewicz, Paweł Kościelniak
Methcathinone (ephedrone), 4-methylmethcathinone (mephedrone) and 3-methylmethcathinone (metaphedrone) are toxicologically-important cathinone derivatives used commonly as designer drugs. In this work we show the first method allowing to separate simultaneously all these molecules in a chiral medium, ensuring good resolution between all enantiomers. Eight cyclodextrins have been tested as potential chiral selectors, the best results were obtained with 2-hydroxyethyl-β-cyclodextrin, unreported so far for efficient separation of cathinones...
July 11, 2018: Electrophoresis
Jan Gimsa, Kanokkan Titipornpun, Marco Stubbe, Ulrike Gimsa
Dielectrophoresis (DEP), electrorotation (ROT), and electro-orientation were used for the dielectric spectroscopy of nucleated three-axial chicken red blood cells (CRBCs). Because the different AC-electrokinetic effects are not mutually independent, their DEP and ROT spectra were combined in ranges separated by the reorientation of the CRBCs in the inhomogeneous linear DEP and circular ROT fields. This behavior can be qualitatively described by a single-shell ellipsoidal model. Whereas in linear fields, the maximum of the Clausius-Mossotti factor along the three axes determines the orientated axis, in circular fields the minimum of the factor determines the axis perpendicularly orientated to the field plane...
July 10, 2018: Electrophoresis
Jin Chen, Xiaoqian Wang, Murtaza Ghulam, Hongxu Chen, Feng Qu
In common partial filling capillary electrophoresis (PF-CE), the capillary contains the selectors plug between the injection and detector end to avoid the selector into the detector zone. To expand this method, we propose a mode of two discontinuous function plugs coupling in-capillary, named as plug-plug partial filling CE (ppPF-CE). Initially, we present the method to predefine the effective length of chiral selector to meet the requirement of enantiomers resolution, which could avoid some experimental procedures...
July 9, 2018: Electrophoresis
Hussain Alghanim, Wensong Wu, Bruce McCord
The goal of this study was to utilize pyrosequencing to identify CpG sites indicative of tobacco smoking using DNA sequences surrounding ten frequently reported smoking-related CpGs. Initially, six genetic loci were investigated including AHRR, 2q37.1, 6p21.33, GFI1, F2RL3, and MYO1G in order to detect novel CpG sites associated with tobacco smoking. The methylation data revealed a set of 23 consecutive CpG sites in blood (Chr5:373,115-Chr5:373,653) that were significantly hypomethylated in current smokers...
June 29, 2018: Electrophoresis
Joseph John Bevelacqua, S M J Mortazavi
Deep space missions, including Mars voyages, are an important area of research. Protection of astronauts' health during these long-term missions is of paramount importance. The paper authored by Szarka et al. entitled "The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1" is indeed a step forward in this effort. Despite numerous strengths, there are some shortcomings in this paper including an incomplete description of the space radiation environment as well as discussion of the resulting biological effects...
June 27, 2018: Electrophoresis
Bolin Zhu, Yaqi Yao, Miaoduo Deng, Zhen Jiang, Qing Li
The enantioseparation of twelve pairs of structurally related 1-aryl-1-indanone derivatives was studied in the normal phase mode using three different polysaccharide type chiral stationary phases, namely Chiralpak IB, Chiralpak IC and Chiralpak ID. n-hexane/2-propanol and n-hexane/ethanol were employed as mobile phases. Among all the investigated chiral columns, Chiralpak IC exhibited the most universal and best enantioseparation ability toward all the racemates, particularly with the mobile phase composed of n-hexane/2-propanol (90/10, v/v)...
June 27, 2018: Electrophoresis
Roger Cardoso Moreira, Brenda M C Costa, Mariana C Marra, Mario H P Santana, Adriano O Maldaner, Élvio D Botelho, Thiago R L C Paixão, Eduardo Mathias Ricther, Wendell Karlos Tomazelli Coltro
This study describes the development of a new analytical method for the separation and detection of cocaine (COC) and its adulterants, or cutting-agents, using ME devices with integrated C4 D. All the experiments were carried out using a glass commercial ME device containing two pairs of integrated sensing electrodes. The running buffer composed of 20 mmol L-1 amino-2- (hydroxymethyl) propane-1,3-diol and 10 mmol L-1 3,4-dimethoxycinnamic acid provided the best separations conditions for COC and its adulterants with baseline resolution (R>1...
June 26, 2018: Electrophoresis
Soheila Chalavi, Ali Reza Fakhari Zavareh, Saeed Nojavan, Peyman Mirzaei
The synergistic effect of two acidic amino acids, aspartic and glutamic acid, on the electrophoretic enantioseparation of four basic drugs was evaluated in the BGE containing a CD and at different pHs. Chlorpheniramine, hydroxyzine, propranolol and tramadol were used as the basic model drugs. However, no enantioseparations were achieved with a BGE containing sole amino acid, but the combined use of an acidic amino acid and a CD showed improved enantioseparations (synergistic effect) compared with the single CD system...
June 26, 2018: Electrophoresis
Sandrine Duvet, Dounia Mouajjah, Romain Péanne, Gert Matthijs, Kimiyo Raymond, Jaak Jaeken, Eva Morava, François Foulquier
Congenital Disorders of Glycosylation are a very heterogeneous group of genetic protein and lipid glycosylation abnormalities. With some 33 reported patients, MAN1B1-CDG belongs to the more frequent causes of CDG-II. MAN1B1 encodes an α (1,2)-mannosidase that removes the terminal mannose residue from the middle branch. Several methods have been proposed to characterize the glycosylation changes. In MAN1B1-CDG, the abnormal accumulating N-glycan structures are mostly absent or found in trace amounts in total human serum...
June 26, 2018: Electrophoresis
Zhiying Wang, Haitao Guo, Meng Chen, Guangbin Zhang, Ruimiao Chang, Anjia Chen
A sensitive, fast and effective method that field-amplified sample stacking (FASS) in capillary electrophoresis has been established for the separation and determination of corynoxine (C) and corynoxine B (CB). Hydroxypropyl-β-CD (HP-β-CD) and tetrabutylammonium-L-glutamic acid (TBA-L-Glu) were used as additives in the separation system. Electrokinetic injection was chosen to introduce sample from inlet at 10 kV for 50 s after a water plug (0.5 psi, 4 s) was injected to permit FASS. The running buffer (pH 6...
June 26, 2018: Electrophoresis
Tejasvi Parupudi, Allen L Garner, Raji Sundararajan
Every forty minutes, one person dies in the USA due to Glioblastoma Multiforme (GBM), a deadly form of brain cancer with an average five-year survival rate less than 3%. The current standard of care for treatment involves surgical resection of the accessible tumor followed by radiation therapy and concomitant chemotherapy. Despite their potency, delivering chemotherapeutic agents to the brain is limited by the highly selective blood brain barrier (BBB), which prevents molecules larger than 500 Da from reaching the brain...
June 26, 2018: Electrophoresis
Tomas Drevinskas, Mantas Stankevičius, Kristina Bimbiraitė-Survilienė, Gintarė Naujokaitytė, Vilma Kaškonienė, Kristina Kondrotienė, Loreta Šernienė, Olga Kornyšova, Mindaugas Malakauskas, Audrius Maruška
Determination of natural preservatives using electrophoretic, or chromatographic techniques in fermented milk products is a complex task due to the following reasons: (i) the concentrations of the analytes can be below the detection limits, (ii) complex matrix and comigrating / coeluting compounds in the sample can interfere with the analytes of the interest, (iii) low recovery of the analytes and (iv) complex sample preparation is necessary. The aim of this study was to apply capillary zone electrophoresis coupled with contactless conductivity detection for the separation and determination of nisin in fermented milk products...
June 23, 2018: Electrophoresis
Tomas Drevinskas, Laimutis Telksnys, Audrius Maruška, Jelena Gorbatsova, Mihkel Kaljurand
One of the main problems of the remote complex sample analysis instrumentation is that such systems are susceptible to temperature fluctuations. Temperature regulation is energetically ineffective, and it is not used in most of the field portable analytical systems. Separations performed in a changing temperature environment provide electropherograms with considerable baseline fluctuations, resulting in significant errors in detection and integration of the peaks. This paper describes electropherogram baseline compensation that is suitable for the capillary electrophoresis - contactless conductivity detection analytical method...
June 23, 2018: Electrophoresis
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