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Ileana-Andreea Ratiu, Hossam Al-Suod, Magdalena Ligor, Tomasz Ligor, Viorica Railean-Plugaru, Bogusław Buszewski
Cyclitols are phytochemicals naturally occurring in plant material which attracted an increasing interest due to multiple medicinal attributes, amongst the most important are the anti-diabetic, antioxidant and anti-cancer properties. Due to their valuable properties, sugars are used in food industry as sweeteners, preservatives, texture modifiers, fermentation substrates, flavoring and coloring agents. In this study we report for the first time the quantitative analysis of sugars and cyclitols isolated from Solidago virgaurea L...
March 15, 2018: Electrophoresis
Sheng Yan, Yuxing Li, Yuanqing Zhu, Minsu Liu, Qianbin Zhao, Dan Yuan, Guolin Yun, Shiwu Zhang, Weijia Wen, Shi-Yang Tang, Weihua Li
This work presents a simple, low-cost method to fabricate semi-circular channels using solder paste, which can amalgamate the cooper surface to form a half-cylinder mould using the surface tension of Sn-Pd alloy (the main component in solder paste). This technique enables semi-circular channels to be manufactured with different dimensions. These semi-circular channels will then be integrated with a polymethylmethacrylate (PMMA) frame and machine screws to create miniaturised, portable microfluidic valves for sequential liquid delivery and particle synthesis...
March 15, 2018: Electrophoresis
Pradipta Kr Das
An analytical model for the electrophoretic speed of DNA translocating through nanopore functionalized with polyelectrolyte brush is developed for the first time. The electrophoretic speed depends on DNA surface potential, applied electric field, viscosity and permittivity of solution along with velocity and electrostatic potential at liquid/ polyelectrolyte layer interface where the interface seemed to behave similar to that of a solid state nanopore wall. Under the limit of Debye-Hückel linearization, the electrostatic potential at liquid/ polyelectrolyte layer interface and at DNA surface have been calculated...
March 15, 2018: Electrophoresis
Mehdi Moini
In the past few years, there has been a significant effort by the forensic science community to develop new scientific techniques for the analysis of forensic evidence. Forensic chemists have been spearheaded to develop information-rich confirmatory technologies and techniques and apply them to a broad array of forensic challenges. The purpose of these confirmatory techniques is to provide alternatives to presumptive techniques that rely on data such as color changes, pattern matching, or retention time alone, which are prone to more false positives...
March 12, 2018: Electrophoresis
Lígia Pimentel, Ana Luiza Fontes, Sofia Salsinha, Manuela Machado, Inês Correia, Ana Maria Gomes, Manuela Pintado, Luís Miguel Rodríguez-Alcalá
Lipids are gaining relevance over the last 20 years, as our knowledge about their role has changed from merely energy/structural molecules to compounds also involved in several biological processes. This led to the creation in 2003 of a new emerging research field: lipidomics. In particular the phospholipids have pharmacological/food applications, participate in cell signalling/homeostatic pathways while their analysis faces some challenges. Their fractionation/purification is, in fact, especially difficult, as they are amphiphilic compounds...
March 8, 2018: Electrophoresis
Meng Li, Yu Zhao, Li Zhou, Jia Yu, Jian Wang, Xingjie Guo
In this work, the enantiomeric separation of ten anticholinergic drugs was first examined on two derivative polysaccharide chiral stationary phases (CSPs), i.e., Chiralpak ID and Chiralpak IA in the normal phase mode. Except for scopolamine hydrobromide, the remaining nine analytes could be completely separated with good resolutions using both columns under the optimized mobile phase conditions. And the enantiomeric discrimination ability of the studied CSPs towards nine analytes was in the order of Chiralpak ID > Chiralpak IA...
March 7, 2018: Electrophoresis
Léa Marlot, Magali Batteau, Karine Faure
Preparative two-dimensional chromatography is gaining interest in the elucidation of complex samples as it allows the recovery of a large number of molecules without the risks inherent to tedious multi-step sample preparation. While the second dimension is often selected to be liquid chromatography, it may be of interest to compare the specificities of two different techniques, namely liquid chromatography and centrifugal partition chromatography, to be used as first dimension. A fair comparison between off-line CPCxLC and prepLCxLC in selective comprehensive mode for preparative purposes is carried out in this study, illustrated by the isolation of five compounds from high-value Edelweiss plant...
March 7, 2018: Electrophoresis
Lucas M Duarte, Luiz H C Adriano, Marcone A L de Oliveira
The precursor compounds related to the bitterness of beer are called α-acids. These compounds are extracted from the hop, which is an important ingredient in the brewing process. These compounds were analyzed by capillary electrophoresis. The electrophoretic method used 160 mmol L-1 of ammonium carbonate (pH 9) as BGE (background electrolyte), a voltage of + 20 kV in a capillary with 50 μm of internal diameter and with a 62.5 cm of total length (54 cm effective). The samples were injected in hydrodynamic mode applying a pressure of 25 mbar for 5 s and the analytes were detected at 230 nm...
March 6, 2018: Electrophoresis
Samah Lahouidak, Rachid Salghi, Mohammed Zougagh, Angel Ríos
A capillary electrophoresis method was developed for the determination of coumarin (COUM), ethyl vanillin (EVA), p-hydroxybenzaldehyde (PHB), p-hydroxybenzoic acid (PHBA), vanillin (VAN), vanillic acid (VANA) and vanillic alcohol (VOH) in vanilla products. The measured concentrations are compared to values obtained by liquid chromatography (LC) method. Analytical results, method precision, and accuracy data are presented and limits of detection for the method ranged from 2 to 5 μg mL-1 . The results obtained are used in monitoring the composition of vanilla flavorings, as well as for confirmation of natural or non-natural origin of vanilla in samples using four selected food samples containing this flavour...
March 6, 2018: Electrophoresis
Fuying Du, Ying-Sing Fung
Methyl parathion (MP) is a highly toxic organophosphate and its exposure may lead to substantial adverse effects to human health. The existence of 4-nitrophenol (4-NP) in the form of free phenol, glucuronide (4-NP-G) or as a sulfate ester (4-NP-S) can be used as biomarkers to assess the duration and extent of MP exposure. In this work, a MC-CE device incorporating post-CE amperometric detection using multi-walled carbon nanotubes (MWNTs) modified carbon fiber microelectrode (CFME) was fabricated and assessed for simultaneous determination of 4-NP, 4-NP-G and 4-NP-S in human urine...
March 3, 2018: Electrophoresis
Akiyoshi Hirayama, Hiroshi Abe, Nozomi Yamaguchi, Sho Tabata, Masaru Tomita, Tomoyoshi Soga
A sheath-flow interface is the most common ionization technique in CE-ESI-MS. However, this interface dilutes the analytes with the sheath liquid and decreases the sensitivity. In this study, we developed a sheathless CE-MS interface to improve sensitivity. The interface was fabricated by making small crack approximately 2 cm from the end of a capillary column fixed on a plastic plate, and then covering the crack with a dialysis membrane to prevent metabolite loss during separation. A voltage for CE separation was applied between the capillary inlet and the buffer reservoir...
March 1, 2018: Electrophoresis
Steven Marczak, Katherine Richards, Zeinab Ramshani, Elaine Smith, Satyajyoti Senapati, Reginald Hill, David B Go, Hsueh-Chia Chang
Exosomes carry microRNA biomarkers, occur in higher abundance in cancerous patients than in healthy ones, and because they are present in most biofluids, including blood and urine, can be obtained non-invasively. Standard laboratory techniques to isolate exosomes are expensive, time-consuming, provide poor purity, and recover on the order of 25% of the available exosomes. We present a new microfluidic technique to simultaneously isolate exosomes and preconcentrate them by electrophoresis using a high transverse local electric field generated by ion-depleting ion-selective membrane...
February 27, 2018: Electrophoresis
Almas F Memon, Amber R Solangi, Saima Q Memon, Arfana Mallah, Najma Memon
Recently, an increasing interest has been observed in ionic liquids (ILs) due to their potentialities in various chemical processes. ILs have some unique properties making them excellent additives in capillary electrophoresis. In this work a simple, rapid, and reliable capillary zone electrophoretic (CZE) method has been developed and validated using 1-butyl-3-methyl imidazolium hexafluorophosphate (BMIM-PF6 ) ionic liquid as a buffer additive for the determination/separation of five flavonoids including hesperedin, epicatechin (EC), epigallocatechin gallate (EGCG), and morin using photodiode array (PDA) detector...
February 27, 2018: Electrophoresis
Lenka Hárendarčíková, Jan Petr
Smartphones have become widely recognized as a very interesting detection and controlling tool in microfluidics. They are portable devices with built-in cameras and internal microprocessors which carry out image processing. In this case, the external computers are not needed and phones can provide fast and accurate results. Moreover, the connectivity of smartphones gives the possibility to share and provide real-time results when needed, whether in health diagnostics, environmental monitoring, immunoassays or food safety...
February 27, 2018: Electrophoresis
Cristina Montealegre, Christian Neusüß
Imaged capillary isoelectric focusing (iCIEF) is a powerful separation technique applied frequently for the analysis of biotherapeutics. However, direct mass spectrometric characterization is usually not possible. Here, focused peaks from an iCIEF system are transferred to a four-port nanoliter valve where peaks of interest are cut and transferred directly (flow injection) or via capillary zone electrophoresis to electrospray mass spectrometry. At first, flow injection coupling was tested with a mixture of peptides showing intraday precision (RSD) of 20...
February 22, 2018: Electrophoresis
Di Li, Jessica Zielinski, Lukasz Kozubowski, Xiangchun Xuan
Cryptococcal meningitis caused by Cryptococcus neoformans is the leading cause of fungal central nervous system infections. Current antifungal treatments for cryptococcal infections are inadequate partly due to the occurrence of drug resistance. Recent studies indicate that the treatment of the azole drug fluconazole changes the morphology of C. neoformans to form enlarged "multimeras" that consist of three or more connected cells/buds. To analyze if these multimeric cells are a prerequisite for C...
February 21, 2018: Electrophoresis
Jan Gimsa
AC fields induce charges at the structural interfaces of particles or biological cells. The interaction of these charges with the field generates frequency-dependent forces that are the basis for AC-electrokinetic effects such as dielectrophoresis (DEP), electrorotation (ROT), and electro-orientation. The effects can be used for the manipulation or dielectric single-particle spectroscopy. The observation of a particular effect depends on the spatial and temporal field distributions, as well as on the shape and the dielectric and viscoelastic properties of the object...
February 21, 2018: Electrophoresis
Victor U Weiss, Monika Golesne, Gernot Friedbacher, Susanne Alban, Wladyslaw W Szymanski, Martina MarchettiDeschmann, Günter Allmaier
Size, size distribution and molecular weight (MW) determination of nanoparticles and i.e. large polymers are of great interest and pose an analytical challenge. In this context, nano electrospray gas-phase electrophoretic mobility molecular analysis (nES GEMMA) is a valuable tool with growing impact. Separation of single-charged analytes according to their electrophoretic mobility diameter (EMD) starting from single-digit EMDs up to several hundred nm diameters is possible. In case of spherical analytes, the EMD corresponds to the dry nanoparticle size...
February 21, 2018: Electrophoresis
Tian Luo, Yue Dong, Shujuan He, Jing Yang, Yuming Dong
In this study, the capillary electrophoresis method with ultraviolet detection was established to directly determine amino acids in vinegar, according to the coordination interaction between amino acids and copper ions. The online sweeping technique was combined to improve the detection sensitivity. The quality of vinegar was evaluated with amino acids as parameters by United Nations Food Agriculture Organization/Word Health Organization amino acids model and principal component analysis. Optimum conditions were obtained under 50 mM CuSO4 and adjusted pH 4...
February 21, 2018: Electrophoresis
Cosima Damiana Calvano, Marco Glaciale, Francesco Palmisano, Tommaso R I Cataldi
Hydrophilic interaction liquid chromatography coupled to negative-ion electrospray linear ion-trap multiple-stage mass spectrometry (HILIC-ESI-MSn , n = 2,3) was used to characterize polar lipids occurring in donkey milk. Besides the detection of abundant phospholipids, the structural characterization and content evaluation of minor glycosphingolipids (GSL) was assessed. We report an unprecedented characterization of 11 hexosyl-ceramides (HexCer), 10 Hex2 Cer and 4 Hex3 Cer. Collision-induced dissociation (CID-MS/MS) spectra in negative ion mode mainly afford information on the monosaccharide number and ceramide constitution (i...
February 20, 2018: Electrophoresis
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