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Qian Liang, Huaguo Chen, Xin Zhou, Qingfang Deng, Enming Hu, Chao Zhao, Xiaojian Gong
Microwave extraction combined ultrasonic pretreatment of flavonoids from Periploca forrestii Schltr. was investigated in this study, extraction process was firstly performed in an ultrasonic cleaner, then treated by microwave irradiation. The optimum ultrasonic time of 25 min was selected by single-factor experiments. A response surface methodology (RSM) has been used to obtain a mathematical model that describes the process and analyzes the significant parameters ethanol concentration 59.92%, liquid to raw materials ratio 21...
January 12, 2017: Electrophoresis
Paolo Fattorini, Carlo Previderé, Ilaria Carboni, Giorgio Marrubini, Solange Sorçaburu-Cigliero, Pierangela Grignani, Barbara Bertoglio, Paolo Vatta, Ugo Ricci
Next generation sequencing (NGS) is the emerging technology in forensic genomics laboratories. It offers higher resolution to address most problems of human identification, greater efficiency and potential ability to interrogate very challenging forensic casework samples. In the present study, a trial set of DNA samples was artificially degraded, by progressive aqueous hydrolysis, and analyzed together with the corresponding unmodified DNA sample and control sample 2800M, to test the performance and reliability of the ForenSeq(TM) DNA Signature Prep kit using the MiSeq Sequencer (Illumina)...
January 12, 2017: Electrophoresis
Kryls Domalaon, Catherine Tang, Alex Mendez, Franky Bernal, Krutarth Purohit, Linda Pham, John Haan, Frank A Gomez
Fabric-based microfluidic fuel cells (MFCs) serve as a novel, cost-efficient alternative to traditional FCs and batteries, since fluids naturally travel across fabric via capillary action, eliminating the need for an external pump and lowering production and operation costs. Building on previous research with Y-shaped paper-based MFCs, fabric-based MFCs mitigate fragility and durability issues caused by long periods of fuel immersion. In the present work we describe a microfluidic fabric-based direct formate fuel cell (DFFC), with 5 M potassium formate and 30% hydrogen peroxide as the anode fuel and cathode oxidant, respectively...
January 12, 2017: Electrophoresis
Ji Li, Rui Hu, Xiaoqing Li, Xin Tong, Dapeng Yu, Qing Zhao
Solid-state nanopore is a promising tool to detect proteins and its complexes. Small proteins (sub-35 kDa) translocate very fast which could not be detected by normal patch-clamp recording instrument due to low temporal resolution. We firstly introduce pressure into protein study and detection. The pressure-derived force, combined with the voltage bias, makes very tiny protein (MW<6.5 kDa) detection possible. Capture rate for Aprotinin is enhanced five times more than that in traditional voltage-driven method by fine tuning of pressure and voltage...
January 9, 2017: Electrophoresis
Yanbo Li, Yukun Ren, Weiyu Liu, Xiaoming Chen, Ye Tao, Hongyuan Jiang
In this study, we develop a nondimensional physical model to demonstrate fluid flow at the micrometer dimension driven by traveling-wave induction electrohydrodynamics (EHD) through direct numerical simulation. In order to realize an enhancement in the pump flow rate as well as a flexible adjustment of anisotropy of flow behavior generated by induction EHD in microchannels, while not adding the risk of causing dielectric breakdown of working solution and material for insulation, a pair of synchronized traveling-wave voltage signals are imposed on double-sided electrode arrays that are mounted on the top and bottom insulating substrate, respectively...
January 9, 2017: Electrophoresis
Qiongdi Zhang, Ming Zhang, Lyas Deghlaf, Jeanne Bataille, Jean Gamby, Anne-Marie Haghiri-Gosnet, Antoine Pallandre
Microfluidics has emerged following the quest for scale reduction inherent to micro and nanotechnologies. By definition, microfluidics manipulates fluids in small channels with dimensions of tens to hundreds of micrometers. Recently, microfluidics has been greatly developed and its influence extends not only the domains of chemical synthesis, bioanalysis and medical researches but also optics and information technology. In this review article, we will shortly discuss an enlightening analogy between electrons transport in electronics and fluids transport in microfluidic channels...
January 6, 2017: Electrophoresis
David Sean, Gary W Slater
We investigate the dynamics of driving a polyelectrolyte such as DNA though a nanopore and into a crosslinked gel. Placing the gel on the trans-side of the nanopore can increase the translocation time while not negatively affecting the capture rates. This setup thus combines the mechanics of gel electrophoresis with nanopore translocation. However, contrary to typical gel electrophoresis scenarios, the effect of the field is localized in the immediate vicinity of the nanopore and becomes negligible inside the gel matrix...
January 6, 2017: Electrophoresis
Yuelin Zhang, Yijie Sha, Kai Qian, Xu Chen, Qin Chen
LB, a bioactive drug, is widely used in treatment of biological diseases. However, due to its poor solution, it is important to find the approach which helps LB to specific biological targets. As the most abundant protein in plasma, HSA plays the role of a carrier of numerous drug ligand. Thus, the interaction between loureirin B (LB) with human serum albumin (HSA) were explored by affinity capillary electrophoresis (ACE), capillary electrophoresis frontal analysis (FA-CE) and pressure-mediated affinity capillary electrophoresis (P-ACE) under simulated physiological conditions (pH = 7...
January 6, 2017: Electrophoresis
Ariana Gonzalez, Michelle Gaines, Frank A Gomez
A microfluidic thread-based analytical device (μTAD) to assess the activity of acetylcholinesterase (AChE) via colorimetric analylsis is described. Fabrication of the device consists of two platforms, both with a nylon thread trifurcated into three channels terminating at open analysis sites at the end of the thread. 5,5'-Dithiobis-(2-nitrobenzoic acid) (DTNB) was spotted and dried on the analysis sites. Acetylthiocholine iodide (ATC) (or cysteine, Cys), is transported through an inlet channel of the nylon thread by capillary action due to the hydrophilic nature of nylon...
January 6, 2017: Electrophoresis
Heidi Lees, Merike Vaher, Mihkel Kaljurand
Novel, fast and simple methods based on RP-HPLC and MEKC with DAD are developed and validated for the qualitative and quantitative determination of five cyclic sulfur mustard (HD) degradation products (1,4-thioxane, 1,3-dithiolane, 1,4-dithiane, 1,2,5-trithiepane, and 1,4,5-oxadithiepane) in water samples. The HPLC method employs a C18 column and an isocratic water-ACN (55:45, v/v) mobile phase. This method enables separation of all five cyclic compounds within 8 min. With the CE method the baseline separation of five compounds was achieved in less than 11 min by applying a simple BGE composed of a 10 mM borate buffer and 90 mM SDS (pH 9...
January 2, 2017: Electrophoresis
Chunye Liu, Frank A Gomez
Neurotransmitters play key roles in cell-to-cell communication. These chemical messengers are involved in many functional processes including growth, reproduction, memory, and behavior. In this communication, we describe a novel microfluidic paper-based analytical device (μPAD) to detect acetylcholinesterase (AChE) activity and inhibitor screening through a colorimetric analysis. The μPAD is easily fabricated via a wax printing process whereby wax is deposited onto the surface of chromatographic paper, and heated to create a hydrophobic barrier...
December 23, 2016: Electrophoresis
Laura Sánchez-Hernández, Cristina Montealegre, Steffen Kiessig, Bernd Moritz, Christian Neusüß
Capillary electrophoresis is an important technique for the characterization of monoclonal antibodies (mAbs), especially in the pharmaceutical context. However, identification is difficult as upscaling and hyphenation of used methods directly to mass spectrometry is often not possible due to separation medium components that are incompatible with MS detection. Here a CE-MS method for the analysis of mAbs is presented analyzing SDS-complexed samples. To obtain narrow and intensive peaks of SDS-treated antibodies, an in-capillary strategy was developed based on the co-injection of positively charged surfactants and methanol as organic solvent...
December 23, 2016: Electrophoresis
Goodwin Will, Alimat Sharizah
The SNPforID consortium identified a panel of 52 SNPs forensic analysis that has been used by several laboratories worldwide. The original analysis of the 52 SNPs was based on a single multiplex reaction followed by two single-base-extension (SBE) reactions each of which was analyzed using capillary electrophoresis. The SBE assays were designed for high throughput genetic analyzers and were difficult to use on the single capillary ABI PRISM 310 Genetic Analyzer and the latest generation 3500 Genetic Analyzer, as sensitivity on the 310 was low and separation of products on the 3500 with POP-7™ was poor...
December 23, 2016: Electrophoresis
Niu J Tan, Leona D J Daim, Amilia A M Jamil, Norhafizah Mohtarrudin, Karuppiah Thilakavathy
Effective protein extraction is essential especially in producing a well-resolved proteome on 2D-gels. A well-resolved placental cotyledon proteome, with good reproducibility, have allowed researchers to study the proteins underlying the physiology and pathophysiology of pregnancy. The aim of this study is to determine the best protein extraction protocol for the extraction of protein from placental cotyledons tissues for a two-dimensional gel electrophoresis (2D-GE). Based on widely used protein extraction strategies, twelve different extraction methodologies were carefully selected, which included one chemical extraction, two mechanical extraction coupled protein precipitations, and nine chemical extraction coupled protein precipitations...
December 19, 2016: Electrophoresis
Tiago Augusto Donegatti, Luís Moreira Gonçalves, Elisabete Alves Pereira
In this work, the use of a novel derivatization agent for the determination of aldehydes (in this particular case: formaldehyde, acetaldehyde, propionaldehyde and valeraldehyde) using micellar electrokinetic chromatography is reported. The derivatization reaction is based on the reaction of aldehydes with benzhydrazide to form the corresponding derivates with maximum absorbance at 250 nm. The experimental conditions of the derivatization reaction as well of the separation were optimized. The adducts were separated with a +22 kV voltage at a temperature of 29 °C...
December 19, 2016: Electrophoresis
Samir H Sadek, F Pimenta, F T Pinho, M A Alves
In this work, we explore two methods to simultaneously measure the electroosmotic mobility in microchannels and the electrophoretic mobility of micron-sized tracer particles. The first method is based on imposing a pulsed electric field, which allows to isolate electrophoresis and electroosmosis at the startup and shutdown of the pulse, respectively. In the second method, a sinusoidal electric field is generated and the mobilities are found by minimizing the difference between the measured velocity of tracer particles and the velocity computed from an analytical expression...
December 19, 2016: Electrophoresis
A Lopez-Oceja, D Gamarra, S Cardoso, L Palencia-Madrid, R A Juste, M M De Pancorbo
The Basque Country is home to the Latxa sheep breed, which is divided in several varieties such as the Latxa Black Face (LBKF) and Latxa Blonde Face (LBLF). Mitochondrial DNA control region analysis of 174 male sheep (97 LBKF and 77 LBLF) was performed with the objective of characterizing the maternal lineages of these two varieties that are the basis to produce the cheese with Idiazabal quality label. The percentage of unique haplotypes was 77.32% in LBKF and 67.53% in LBLF. Most of the individuals were classified into B haplogroup (98...
December 19, 2016: Electrophoresis
Van Bac Dang, Sung-Jin Kim
Microfluidic systems with modular components are attractive alternatives to monolithically integrated microfluidic systems because of their flexibility. In this study, we apply the modular concept on a water-head-pressure-driven microfluidic oscillator and obtain a widely tunable flow rate and fluidic switching period. Modular fluidic resistors can be easily mounted onto and demounted from a main chip by means of plastic male connectors. The connectors enable a leak-free connection between the modular resistors and main chip (leakage pressure > 140 kPa)...
December 16, 2016: Electrophoresis
Carolina Núñez, Miriam Baeta, Nerea Ibarbia, Urko Ortueta, Susana Jiménez-Moreno, José Luis Blazquez-Caeiro, Juan José Builes, Rene J Herrera, Begoña Martínez-Jarreta, Marian M de Pancorbo
A Y-STR multiplex system has been developed with the purpose of complementing the widely used 17 Y-STR haplotyping (AmpFlSTR Y Filer® PCR Amplification kit) routinely employed in forensic and population genetic studies. This new multiplex system includes six additional STR loci (DYS576, DYS481, DYS549, DYS533, DYS570, and DYS643) to reach the 23 Y-STR of the PowerPlex® Y23 System. In addition, this kit includes the DYS456 and DYS385 loci for traceability purposes. Male samples from 625 individuals from ten worldwide populations were genotyped, including three sample sets from populations previously published with the 17 Y-STR system to expand their current data...
December 16, 2016: Electrophoresis
Chunyan Zhou, Jingjing Deng, Guoyue Shi, Tianshu Zhou
Tetracyclines are a group of broad spectrum antibiotics widely used in animal husbandry to prevent and treat diseases. However, the improper use of tetracyclines may result in the presence of their residues in animal tissues or waste. Recently, great attention has been drawn towards the green solvents ionic liquids. Ionic liquids have been employed as coating material to modify the electroosmotic flow in capillary electrophoresis. In this study, a functionalized ionic liquid, mono-6-deoxy-6-(3-methylimidazolium)-β-cyclodextrin tosylate, was synthesized and used for the simultaneous separation and quantification of tetracyclines by capillary electrophoresis...
December 16, 2016: Electrophoresis
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