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Zoltán-István Szabó, Réka Gál, Levente Szőcs, Róbert Ludmerczki, Daniela-Lucia Muntean, Béla Noszál, Gergő Tóth
The enantiomers of praziquantel, the drug of choice in schistosomiasis, were separated by electrokinetic chromatography with cyclodextrins. Nine anionic cyclodextrins were screened for their ability to discriminate between the uncharged enantiomers. Seven investigated selectors presented chiral interactions with the enantiomers, these cases being interpreted in terms of stability constants and complex mobilities. The best results were delivered by sulfated-β-cyclodextrin, where quasi-equal stability constants were accompanied by extreme selectivity values and was explained on the basis of highly different mobilities of the transient diastereomeric complexes...
February 21, 2017: Electrophoresis
Friederike A Sandbaumhüter, Regula Theurillat, Wolfgang Thormann
The racemic N-methyl-d-aspartate receptor antagonist ketamine is used in anesthesia, analgesia and the treatment of depressive disorders. It is known that interactions of hydroxylated norketamine metabolites and 5,6-dehydronorketamine (DHNK) with the α7 -nicotinic acetylcholine receptor and the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor are responsible for the antidepressive effects. Ketamine and its first metabolite norketamine are not active on these receptors. As stereoselectivity plays a role in ketamine metabolism, a cationic capillary electrophoresis based method capable of resolving and analyzing the stereoisomers of four hydroxylated norketamine metabolites, norketamine and DHNK was developed...
February 18, 2017: Electrophoresis
Weiyu Liu, Yukun Ren, Ye Tao, Yanbo Li, Xiaoming Chen
We report herein controllable rotating behavior of an individual dielectric micro-rod driven by a background rotating electric field. By disposing or removing structured floating microelectrode, the rigid rod suspended in electrolyte solution accordingly exhibits co-field or anti-field rotating motion. In the absence of the ideally polarizable metal surface, the dielectric rod rotates opposite to propagation of electric field, with the measured rotating rate much larger than predicted by Maxwell-Wager interfacial polarization theory incorporating surface conduction of fixed bond charge...
February 18, 2017: Electrophoresis
Michael T Smith, Shu Zhang, Troy Adams, Byron DiPaolo, Jennifer Dally
Capillary and microfluidic chip electrophoresis technologies are heavily utilized for development, characterization, release, and stability testing of biopharmaceuticals. Within the biopharmaceutical industry, CE-SDS and M-CGE are commonly used for purity determination by separation and quantitation of size-based variants. M-CGE is used primarily as a R&D tool for product and process development, while cGMP release and stability testing applications are commonly reserved for CE-SDS. This paper describes the establishment of a M-CGE platform method to be used for R&D and cGMP applications, including release and stability testing, for monoclonal antibodies...
February 17, 2017: Electrophoresis
Foram Ranjeet Madiyar, Sherry Basset, Omer Farooq, Stefan Rothenburg, Christopher Culbertson, Jun Li
This paper reports the capture and detection of vaccinia virus particles based on AC dielectrophoresis (DEP) and electrochemical impedance measurements employing an embedded vertically aligned carbon nanofiber (VACNF) nanoelectrode array (NEA) versus a macroscopic indium-tin-oxide (ITO) transparent electrode in a "points-and-lid" configuration. The nano-DEP device was fabricated by bonding two SU-8 covered electrodes patterned using photolithography. The bottom electrode contains a 200 × 200 μm(2) active region on a randomly distributed NEA and the top electrode contains a microfluidic channel in SU-8 spin-coated on ITO to guide the flow of the virus solution...
February 17, 2017: Electrophoresis
Jiao Zeng, Shengquan Liu, Menglin Wang, Shouzhuo Yao, Yingzhuang Chen
In this work, a porous structure and good permeability monolithic column was polymerized in UV transparent fused-silica capillaries via photo-initiated thiol-ene click polymerization of 2, 4, 6, 8-tetravinyl-2, 4, 6, 8- tetramethylcyclotetrasiloxane (TMTVS), pentaerythritol tetra(3-mercaptopropionate) (PETMP), itaconic acid, respectively, in the presence of porogenic solvents (tetrahydrofuran and methanol) and a initiator (2, 2-dimethoxy-2-phenylacetophenone) (DMPA) within 30 min. The physical properties of this monolith were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and nitrogen adsorption/desorption measurements...
February 17, 2017: Electrophoresis
Chadin Kulsing, Yuanzhong Yang, Jamil M Chowdhury, Reinhard I Boysen, Milton T W Hearn
This investigation demonstrates the application of a new peak sharpening technique to improve the separation of difficult-to-resolve racemic mixtures in capillary electro-chromatography. Molecularly imprinted porous layer open tubular (MIP-PLOT) capillaries, prepared by a layer-on-layer polymerization approach with Z-l-Asp-OH as the template, were selected to validate the approach. SEM revealed that the polymer film thickness can be varied by changes in both the polymer composition and the layer-on-layer regime...
February 17, 2017: Electrophoresis
Hooi Sin Tan, Richard P Jacoby, Meilina Ong-Abdullah, Nicolas L Taylor, Susan Liddell, Wong Wei Chee, Chiew Foan Chin
Oil palm is one of the most productive oil bearing crops grown in Southeast Asia. Due to the dwindling availability of agricultural land and increasing demand for high yielding oil palm seedlings, clonal propagation is vital to the oil palm industry. Most commonly, leaf explants are used for in vitro micropropagation of oil palm and to optimise this process it is important to unravel the physiological and molecular mechanisms underlying somatic embryo production from leaves. In this study, a proteomic approach was used to determine protein abundance of mature oil palm leaves...
February 15, 2017: Electrophoresis
Carla M Teglia, María S Cámara, Luciana Vera-Candioti
In the previously published part of this study, we detailed a novel strategy based on dispersive liquid-liquid microextraction to extract and preconcentrate nine fluoroquinolones in porcine blood. Moreover, we presented the optimized experimental conditions to obtain complete CE separation between target analytes. Consequently, this second part reports the validation of the developed method to determine flumenique, difloxacin, enrofloxacin, marbofloxacin, ofloxacin, ciprofloxacin, through univariate calibration, and enoxacin, danofloxacin, and gatifloxacin through multivariate curve resolution analysis...
February 8, 2017: Electrophoresis
Sabrina B Mammana, Paula Berton, Alejandra B Camargo, Gustavo E Lascalea, Jorgelina C Altamirano
An analytical methodology based on co-precipitation-assisted coacervative extraction (cop-CAE) coupled to High-Performance Liquid Chromatography (HPLC-UV) was developed for determination of five organophosphorus pesticides (OPPs), including fenitrothion, guthion, parathion, methidathion and chlorpyrifos, in water samples. It involves a green technique leading to an efficient and simple analytical methodology suitable for high throughput analysis. Relevant physicochemical variables were studied and optimized on the analytical response of each OPP...
February 8, 2017: Electrophoresis
Bo Yang, Jie Zhou, Yong Wang, Jian Tang, Weihua Tang
The enantioseparations of 12 isoxazoline racemates were explored with four perphenylcarbamate cyclodextrin (CD) clicked chiral stationary phases (CSPs) in high performance liquid chromatography (HPLC). The results demonstrated that the functionalities on phenylcarbamate moiety greatly determined the chiral separation ability of CD clicked CSPs. Among of them, per(3-chloro-4-methylphenylcarbamate) CD clicked CSP (CCC3M4-CSP) exhibited the best enantioseparation ability, affording 4ClPh-OPr with a chiral resolution over 20 in ternary eluent mobile phases...
February 8, 2017: Electrophoresis
Jong Min Lee, Hye In Seo, Jun Hyuk Bae, Bong Geun Chung
We developed the photo-crosslinkable hydrogel microfluidic co-culture device to study photothermal therapy and cancer cell migration. To culture MCF7 human breast carcinoma cells and metastatic U87MG human glioblastoma in the microfluidic device, we used 10 w/v% gelatin methacrylate (GelMA) hydrogels as a semi-permeable physical barrier. We demonstrated the effect of gold nanorod on photothermal therapy of cancer cells in the microfluidic co-culture device. Interestingly, we observed that metastatic U87MG human glioblastoma largely migrated toward vascular endothelial growth factor (VEGF)-treated GelMA hydrogel-embedding microchannels...
February 7, 2017: Electrophoresis
Justyna Godyń, Michalina Hebda, Anna Więckowska, Krzysztof Więckowski, Barbara Malawska, Marek Bajda
Lipophilicity as one of the most important physicochemical properties of the biologically active compounds is closely related to their pharmacokinetic parameters and therefore, it is taken into account at the design stage of new drugs. Among the novel, fast and reliable methods for determination of the lipophilicity of compounds micellar electrokinetic chromatography (MEKC) is considered to be an appropriate one for bioactive molecules, as it closely mimics the physiological conditions. In this paper MEKC was used for the estimation of log P values for 49 derivatives of phthalimide, tetrahydroisochinoline and indole, designed and synthesized as potential anti-Alzheimer's agents with cholinesterase inhibitory activity...
February 7, 2017: Electrophoresis
Yanfang Liu, Huidan Liao, Ying Liu, Juanjuan Guo, Yi Sun, Xiaoliang Fu, Ding Xiao, Jifeng Cai, Lingmei Lan, Pingli Xie, Lagabaiyila Zha
Nonbinary single-nucleotide polymorphisms (SNPs) are potential forensic genetic markers because that their discrimination power is greater than that of normal binary SNPs, and that they can detect highly degraded samples. We previously developed a nonbinary SNP multiplex typing assay. In the present study, we selected additional 20 nonbinary SNPs from the NCBI SNP database and verified them through pyrosequencing. These 20 nonbinary SNPs were analyzed using the fluorescent-labeled SNaPshot multiplex SNP typing method...
February 6, 2017: Electrophoresis
Paola Peluso, Victor Mamane, Emmanuel Aubert, Sergio Cossu
The term Atropisomerism defines a type of enantiomerism that arises from hindered rotation around an axis that makes two rotational isomers enantiomers. Atropisomeric compounds are present in nature, being important building blocks of many biologically active compounds. Atropisomers have been extensively studied in environmental and toxicological chemistry and, in particular, separating them has become a need in specific fields as organic synthesis and pharmaceutical research. High-performance liquid chromatography has been fruitfully applied in this context, and, despite HPLC separation on chiral stationary phase (CSP) is considered as mature technology nowadays, in the last years several advancements and applications contributed to study compounds where axial chirality is of utmost importance...
February 6, 2017: Electrophoresis
Barbaros Çetin, S Doğan Öner, Besim Baranoğlu
Dielectrophoresis (DEP) is a very popular technique for microfluidic bio-particle manipulation. For the design of a DEP based microfluidic device, simulation of the particle trajectory within the micro-channel network is crucial. There are basically two approaches: (i) point-particle approach and (ii) finite-sized particle approach. In this study, many aspects of both approaches are discussed for the simulation of DC-DEP, AC-DEP and traveling-wave DEP applications. Point-particle approach is implemented using Lagrangian tracking method, and finite-sized particle is implemented using boundary element method (BEM)...
February 6, 2017: Electrophoresis
Hasan Hüseyin Kazan, Cagri Urfali-Mamatoglu, Ufuk Gündüz
Electrophoretic mobility is a physical phenomenon defining the mobility of charged particles in a solution under applied electric field. As charged biological systems, living cells including both prokaryotes and eukaryotes have been assessed in terms of electrophoretic mobility to decipher their electrochemical structure. Moreover, determination of electrophoretic mobility of living cancer cells have promoted the advance exploration of the nature of the cancer cells and separation of cancer cells from normal ones under applied electric field...
February 3, 2017: Electrophoresis
Wenjie Liu, Ronghua Li, Habtamu Ayalew, Yanshi Xia, Guihua Bai, Guijun Yan, Kadambot H M Siddique, Peiguo Guo
Silver staining is one of the widely used methods for DNA fragment detection in biological research. Silver staining protocols have been steadily optimized to improve detection efficiency. This research reports a continuous effort to simplify the existing silver staining protocols, lower experiment cost, and improve DNA detection sensitivity and image clarity. The new method only requires three reagents (silver nitrate, sodium hydroxide and formaldehyde) and 6-7 minutes with high detection sensitivity to visualize as low as 14...
January 31, 2017: Electrophoresis
Anil Koklu, Ahmet C Sabuncu, Ali Beskok
Dielectrophoretic motions of Saccharomyces Cerevisiae (yeast) cells and colloidal gold are investigated using electrochemically modified electrodes exhibiting fractal topology. Electrodeposition of gold on electrodes generated repeated patterns with a fern-leaf type self-similarity. A particle tracking algorithm is used to extract dielectrophoretic particle velocities using fractal and planar electrodes in two different medium conductivities. The results show increased dielectrophoretic force when using fractal electrodes...
January 28, 2017: Electrophoresis
Murthy Jonnada, Guadalupe Davila El Rassi, Ziad El Rassi
Fatty acids (FAs) have been selectively derivatized with a fluorescent tag, 6-aminoquinoline (6AQ), which yielded fluorescent FA-6AQ derivatives that have excitation (λexc = 270 nm) and emission (λemi = 495 nm) wavelengths that are farther apart. This precolumn derivatization is characterized by its simplicity occurring at room temperature between the carboxylic acid group of the FA and the amino group of 6AQ in the presence of a non-aqueous soluble carbodiimide coupling agent such as the N,N´-dicyclohexylcarbodiimide...
January 28, 2017: Electrophoresis
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