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Marcelina Pyschik, Marcel Klein-Hitpaß, Sabrina Girod, Martin Winter, Sascha Nowak
In this study, an optimized method using capillary electrophoresis (CE) with a direct contactless conductivity detector (C(4) D) for a new application field is presented for the quantification of fluoride in common used lithium - ion battery (LIB) electrolyte using LiPF6 in organic carbonate solvents and in ionic liquids (ILs) after contacted to Li metal. The method development for finding the right buffer and the suitable CE conditions for the quantification of fluoride was investigated. The results of the concentration of fluoride in different LIB electrolyte samples were compared to the results from the ion-selective electrode (ISE)...
October 22, 2016: Electrophoresis
Shobha Devi, Po-Hung Wu, Pei-Yu Chu, Yue-Pei Liu, Hsin-Lin Wu, Yen-Peng Ho
The impact of microwave irradiation on the in-solution digestion processes and the detection limit of proteins are systematically studied. Kinetic processes of many peptides produced through the trypsin digestion of various proteins under microwave heating at 50°C were investigated with MALDI-MS. This study also examines the detection limits and digestion completeness of individual proteins under microwave heating at 50°C and at different time intervals (1, 5 and 30 min) using LC-MS. We conclude that, if the peptides without missed cleavage dictate the detection limit, conventional digestion will lead to a better detection limit...
October 22, 2016: Electrophoresis
Magdalena M Bus, Ognjen Karas, Marie Allen
The capillary electrophoresis (CE) technology is commonly used for fragment length separation of markers in forensic DNA analysis. In this study, pyrosequencing technology was used as an alternative and rapid tool for the analysis of biallelic InDel (insertion/deletion) markers for individual identification. The DNA typing is based on a subset of the InDel markers that are included in the Investigator(®) DIPplex Kit, which are sequenced in a multiplex pyrosequencing analysis. To facilitate the analysis of degraded DNA, the polymerase chain reaction (PCR) fragments were kept short in the primer design...
October 20, 2016: Electrophoresis
Tereza Tůmová, Lenka Monincová, Václav Čeřovský, Václav Kašička
Capillary electrophoresis (CE) was employed for the determination of thermodynamic acidity constants (pKa ) and actual ionic mobilities of polycationic antimicrobial peptides (AMPs). The effective electrophoretic mobilities of AMPs were measured by CE in a series of the background electrolytes within a wide pH range (2.00-12.25), at constant ionic strength (25 mM) and ambient temperature, using polybrene coated fused silica capillaries to suppress sorption of cationic AMPs to the capillary wall. Eventually, Haarhoff-Van der Linde peak fitting function was used for the determination of correct migration times of some AMPs peaks that were distorted by electromigration dispersion...
October 19, 2016: Electrophoresis
Alireza Banazadeh, Lucas Veillon, Kerry M Wooding, Masoud Zabet, Yehia Mechref
Glycosylation is one of the most common post-translational modifications of proteins and plays essential roles in various biological processes, including protein folding, host-pathogen interaction, immune response, and inflammation and aberrant protein glycosylation is a well-known event in various disease states including cancer. As a result, it is critical to develop rapid and sensitive methods for the analysis of abnormal glycoproteins associated with diseases. Mass spectrometry in conjugation with different separation methods, such as capillary electrophoresis, ion mobility, and high performance liquid chromatography, has become a popular tool for glycoprotein analysis, providing highly informative fragments for structural identification of glycoproteins...
October 18, 2016: Electrophoresis
Thitika Kitpipit, Phuvadol Thanakiatkrai, Kitichaya Penchart, Kanita Ouithavon, Chutamas Satasook, Adrian Linacre
Despite continuous conservation efforts by national and international organizations, the populations of the three extant elephant species are still dramatically declining due to the illegal trade in ivory leading to the killing of elephants. A requirement to aid investigations and prosecutions is the accurate identification of the elephant species from which the ivory was removed. We report on the development of the first fully validated multiplex PCR-electrophoresis assay for ivory DNA analysis that can be used as a screening or confirmatory test...
October 18, 2016: Electrophoresis
Woong Choi, Gyoo Yeol Jung
For the development of clinically useful genotyping methods for single nucleotide polymorphisms (SNPs), accuracy, simplicity, sensitivity, and cost-effectiveness are the most important criteria. Among the methods currently being developed for SNP genotyping technology, the ligation-dependent method is considered the simplest for clinical diagnosis. However, sensitivity is not guaranteed by the ligation reaction alone, and analysis of multiple targets is limited by the detection method. Although CE is an attractive alternative to error-prone hybridization-based detection, the multiplex assay process is complicated because of the size-based DNA separation principle...
October 18, 2016: Electrophoresis
Seim Timung, Joydip Chaudhuri, Manash Pratim Borthakur, Tapas Kumar Mandal, Gautam Biswas, Dipankar Bandyopadhyay
We report a facile and non-invasive way to disintegrate a microdroplet into a string of further miniaturized ones under the influence of an external electrohydrodynamic field inside a microchannel. The deformation and breakup of the droplet was engendered by the Maxwell's stress originating from the accumulation of induced and free charges at the oil-water interface. While at smaller field intensities, e.g. less than 1 MV/m, the droplet deformed into a plug, at relatively higher field intensities, e.g. ∼1...
October 17, 2016: Electrophoresis
Naveen Maddukuri, Qiyang Zhang, Ning Zhang, Maojun Gong
Laser-induced fluorescence (LIF) detection often requires labelling of analytes with fluorophores; and fast fluorescent derivatization is valuable for high-throughput analysis with flow-gated capillary electrophoresis (CE). Here, we report a fast fluorescein-labelling scheme for amino neurotransmitters, which were then rapidly separated and detected in flow-gated CE. This scheme was based on the reaction between primary amines and o-phthalaldehyde (OPA) in the presence of a fluorescent thiol, 2-[(5-fluoresceinyl)aminocarbonyl]ethyl mercaptan (FACE-SH)...
October 17, 2016: Electrophoresis
Paula Berton, Sabrina B Mammana, Daniela A Locatelli, Nerina B Lana, María B Hapon, Alejandra B Camargo, Jorgelina C Altamirano
Ultrasound assisted extraction (UAE), cloud point extraction (CPE) and ultrasound back-extraction (UABE) techniques have been coupled for lixiviation, preconcentration and cleanup of polybrominated diphenyl ethers (PBDEs) from milk samples for determination by gas chromatography-electron capture detection (GC-ECD). Physicochemical parameters that affect the efficiency of the extraction system were investigated using a design of experiments based on multivariate statistical tools, and considering the sample matrix along the development...
October 14, 2016: Electrophoresis
Piotr Kowalski, Ilona Olędzka, Alina Plenis, Tomasz Bączek
An indirect UV detection method based on capillary electrophoresis was developed and validated to determinate 12. metal cations, including alkali, alkaline earth, transition metal, and ammonium. In this paper a new electrolyte system (pH 4.22) contained 20 mM benzimidazole (as co-ion), 75 mM acetic acid (as a counter-ion) as well as 0.6 mM 18-crown-6 ether was applied. The metal ions were completely separated within 8 min under hydrodynamic mode injection with a running voltage of 20 kV at 25 ± 0.1°C. Additional use of the dynamic double coating method enabled to get an excellent repeatability of migration times and quantitative parameters for all analytes...
October 14, 2016: Electrophoresis
H H Quan, M Li, Y Huang, J-H Hahn
This paper demonstrates a novel compartmentalized sampling/labelling method and its separation techniques using a hydrophobic ionic liquid (IL) - 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)-imidate (BmimNTf2 ) - as the immiscible phase, which is capable of minimizing signal losses during microchip capillary electrophoresis (MCE). The MCE device consists of a silica tube connected to a straight polydimethylsiloxane (PDMS) separation channel. Poly(diallyldimethylammonium chloride) (PDDAC) was coated on the inner surface of channel to ease the introduction of IL plugs and enhance the IL wetting on the PDMS surface for sample releasing...
October 13, 2016: Electrophoresis
Mohammad Azim, Ali Malekpourkoupaei, Wenmin Ye, Abebaw B Jemere, Jed Harrison
The separation behavior of 6.5-66 kDa proteins in silica particle array-based sieves formed by colloidal self-assembly (CSA) in microchips is reported across a pore size range of 22-103 nm. The protein separation and resolution improves markedly with decreasing pore size. The variation of electrophoretic mobility with molecular weight of SDS-protein complexes and with particle size was evaluated using the Ogston sieving equation for a random pore gel structure, and using the modified Giddings developed by Wirth equation for uniform pores structure...
October 13, 2016: Electrophoresis
Renuka Rathnasekara, Shantipriya Khadka, Murthy Jonnada, Ziad El Rassi
This review article is a continuation of the previous reviews on the area of monolithic columns covering the progress made in the field over the last couple of years from the beginning of the second half of 2014 until the end of the first half of 2016. It summarizes and evaluates the evolvement of both polar and nonpolar organic monolithic columns and their use in hydrophilic interaction-liquid chromatography and capillary electrochromatography (HILIC and HICEC) and reversed-phase chromatography (RPC) and RP-CEC...
October 12, 2016: Electrophoresis
Shumin Chen, Meiling Wen, Shujie Bu, Ahui Wang, Ya Jin, Wen Tan
Plasma samples from adult male rats were separated by nondenaturing micro 2DE and a reference gel was selected, on which 136 CBB-stained spots were numbered and subjected to in-gel digestion and quantitative LC-MS/MS. The analysis provided the assignment of 1-25 (average eight) non-redundant proteins in each spot and totally 199 proteins were assigned in the 136 spots. About 40% of the proteins were detected in more than one spot and 15% in more than 10 spots. We speculate this complexity arose from multiple causes including protein heterogeneity, overlapping of protein locations and formation of protein complexes...
October 12, 2016: Electrophoresis
J Bodnar, L Hajba, A Guttman
Surface modification of the inner capillary wall in CE of proteins is frequently required to alter electroosmotic flow and to prevent protein adsorption. Manual protocols for such coating techniques are cumbersome. In this paper an automated covalent linear polyacrylamide coating and regeneration process is described to support long term stability of fused silica capillaries for protein analysis. The stability of the resulting capillary coatings was evaluated by a large number of separations using a three-protein test mixture in pH 6 and pH 3 buffer systems...
October 12, 2016: Electrophoresis
Ziad El Rassi, Chanida Puangpila
This review article is an update of our previous one by C. Puangpila, E. Mayadunne and Z. El Rassi, Electrophoresis 2015, 36, 238-252. Similarly to the previous article, this review has two main topics, including (i) proteomic sample preparation (e.g., depletion of high abundance proteins, reduction of the protein dynamic concentration range and enrichment of a particular sub-proteome), and (ii) the subsequent chromatographic and/or electrophoretic pre-fractionation prior to protein separation and identification by LC-MS/MS...
October 12, 2016: Electrophoresis
Sheng Yan, Jun Zhang, Dan Yuan, Weihua Li
Microfluidics, which is classified as either active or passive, is capable of separating cells of interest from a complex and heterogeneous sample. Active methods utilise external fields such as electric, magnetic, acoustic, and optical to drive cells for separation, while passive methods utilise channel structures, intrinsic hydrodynamic forces, and steric hindrances to manipulate cells. However, when processing complex biological samples such as whole blood with rare cells, separation with a single module microfluidic device is difficult...
October 8, 2016: Electrophoresis
Rawi Ramautar, Govert W Somsen, Gerhardus J de Jong
Capillary electrophoresis-mass spectrometry (CE-MS) can be considered a useful analytical technique for the global profiling of (highly) polar and charged metabolites in various samples. Over the past few years, significant advancements have been made in CE-MS approaches for metabolomics studies. In this paper, which is a follow-up of a previous review paper covering the years 2012-2014 (Electrophoresis 2015, 36, 212-224), recent CE-MS strategies developed for metabolomics covering the literature from July 2014 to June 2016 are outlined...
October 8, 2016: Electrophoresis
Anders Buchard, Marie-Louise Kampmann, Lena Poulsen, Claus Børsting, Niels Morling
The HID-Ion AmpliSeq™ Identity Panel is a next-generation sequencing assay with 90 autosomal and 34 Y-chromosome SNPs that are amplified in one PCR step and subsequently sequenced using the Ion Personal Genome Machine (Ion PGM™) System. This assay was validated for relationship testing in our ISO 17025 accredited laboratory in 2015. Here, the essential parts of the validation report submitted to the Danish Accreditation Fund are presented. A total of 100 unrelated Danes were typed in duplicates and the locus balance, heterozygote balance (Hb) and noise levels were analysed in detail...
October 6, 2016: Electrophoresis
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