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Kemilly M P Pinheiro, Roger C Moreira, Kariolanda C A Rezende, Márcio Talhavini, Lucio P L Logrado, José A F Baio, Marcos R V Lanza, Wendell K T Coltro
This study describes the development of an analytical methodology based on the use of microchip electrophoresis (ME) devices integrated with capacitively coupled contactless conductivity detection (C4 D) for the separation and detection of inorganic anions in post-blast explosive residues. The best separation condition was achieved using a running buffer composed of 35 mmol/L lactic acid, 10 mmol/L histidine and 0.070 mmol/L cetyl(trimethyl ammonium) bromide. For C4 D measurements, the highest sensitivity was obtained applying a 700 kHz sinusoidal wave with excitation voltage of 20 Vpp ...
November 8, 2018: Electrophoresis
Lilla Makszin, Péter Kustán, Balázs Szirmay, Csilla Páger, Emerencia Mező, Krisztina I Kalács, Vera Pászthy, Erzsébet Györgyi, Ferenc Kilár, Andrea Ludány, Tamás Kőszegi
Perchloric acid (PCA) precipitation is a well-known method for the separation of heavily glycosylated proteins and for reducing the masking effect of major serum proteins. The aim of this study is to characterize PCA-soluble serum proteins in healthy individuals and in patients with systemic inflammatory diseases, such as Crohn's disease (CD) and sepsis. A PCA precipitation protocol was prepared and adapted to the analytical methods. After PCA treatment of the serum, the soluble proteins in the supernatants were analyzed by SDS-PAGE and by microchip gel electrophoresis (MGE)...
November 8, 2018: Electrophoresis
Yusheng Fu, Hong Ji
In this paper, we demonstrated an integrated digital image processing framework that is training-free for high throughput beads or biological cells detection and enumeration by the bead aggregation splitting algorithm. By making contour extraction processing, the aggregated beads can be clearly split for precise counting. It can be potentially embedded on-chip in a miniaturized medical equipment to automatically adjust illumination condition and de-noise. This study demonstrates that the existing hematological analysis can be updated from manual classification and counting by high-speed and precise machine-based programs...
November 1, 2018: Electrophoresis
Natalia M Neris, Ricardo D Guevara, Ariana Gonzalez, Frank A Gomez
In this paper we describe the fabrication of novel three-dimensional multilayered microfluidic paper-based analytical devices (3D-μPADs) and a 3D microfluidic thread/paper-based analytical device (3D-μTPAD) to detect glucose and bovine serum albumin (BSA) through colorimetric assays. The 3D-μPAD and 3D-μTPAD consisted of three (wax, heat pressed wax-printed paper, single-sided tape) and four (hole-punched single-sided tape, blank chromatography circles, heat-pressed wax-printed paper, hole-punched single-sided tape containing trifurcated thread) layers, respectively...
November 1, 2018: Electrophoresis
Zhenyu Song, Mengqi Li, Bao Li, Yimo Yan, Yongxin Song
A novel microfluidic method of continually detecting and counting beads-labeled cells from a cell mixture without fluorescence labeling was presented in this paper. The detection system is composed of a microfluidic chip (with a permanent magnet inserted along the channel), a signal amplification circuit and a LabView® based data acquisition device. The microfluidic chip can be functionally divided into separation zone and detection zone. By flowing the pre-labeled sample solution, the target cells will be sequentially separated at the separation zone by the permanent magnet and detected and counted at the detection zone by a microfluidic resistive pulse sensor...
October 31, 2018: Electrophoresis
Yanjuan Wang, Junsheng Wang, Jie Meng, Gege Ding, Zhi Shi, Ruoyu Wang, Xiaohui Zhang
In early diagnosis of lung cancer, a polarization microscopy is a powerful tool of obtained the optical information of biological tissues. In this paper, a new microfluidic polarization imaging and analysis method was proposed for the detection and classification of cancer-associated fibroblasts (CAF) and two kinds of non-small cell lung cancer cells, A549 and H322. A polarizing microscopy system was constructed based on a commercial microscope to obtained 3*3 Mueller matrix of cells. Based on the Muller matrix decomposition algorithm and analysis in spatial domain and frequency domain, appropriate classification parameters were selected for the characterization of different polarization characteristics of cells...
October 31, 2018: Electrophoresis
Jiafeng Yao, Guiping Zhu, Tong Zhao, Masahiro Takei
Microfluidic device embedding electrodes realizes cell manipulation with the help of dielectrophoresis. Cell manipulation is an important technology for cell sorting and cell population purification. Till now, the theory of dielectrophoresis has been greatly developed. Microfluidic devices with various arrangements of electrodes have been reported, from the beginning of the single non-uniform electric field to the later multiple physical fields. This paper reviews the research status of microfluidic device embedding electrodes for cell manipulation based on dielectrophoresis...
October 30, 2018: Electrophoresis
Teng Zhou, Xiang Ji, Liuyong Shi, Xianman Zhang, Yongbo Deng, Sang Woo Joo
The dielectrophoretic (DEP) choking phenomenon is revisited for Janus particles that are transported electrokinetically through a microchannel constriction by a direct-current (DC) electric field. The negative DEP force that would block a particle with a diameter significantly smaller than that of the constriction at its inlet is seen to be relaxed by the rotation of the Janus particle in a direction that minimizes the magnitude of the DEP force. This allows the particle to pass through the constriction completely...
October 29, 2018: Electrophoresis
Hasliza Hassan, Mohd Din Amiruddin, Wolfram Weckwerth, Umi Salamah Ramli
Palm oil is an edible vegetable oil derived from lipid-rich fleshy mesocarp tissue of oil palm (Elaeis guineensis Jacq.) fruit and is of global economic and nutritional relevance. While the understanding of oil biosynthesis in plants is improving, the fundamentals of oil biosynthesis in oil palm still require further investigations. To gain insight into the systemic mechanisms that govern oil synthesis during oil palm fruit ripening, the proteomics approach combining gel-based electrophoresis and mass spectrometry was used to profile protein changes and classify the patterns of protein accumulation during these complex physiological processes...
October 29, 2018: Electrophoresis
Ling Xia, Naoki Yanagisawa, Rajesh Deb, Debashis Dutta
On-chip generation of pressure-gradients via electrokinetic means can offer several advantages to microfluidic assay design and operation in a variety of applications. In this article, we describe a simple approach to realizing this capability by employing a polyacrylamide-based gel-structure fabricated within a fluid reservoir located at the terminating end of a microchannel. Application of an electric field across this membrane has been shown to block a majority of the electroosomotic flow generated within the open duct yielding a high pressure at the channel-membrane junction...
October 29, 2018: Electrophoresis
Cody Masters, Jocelyn Dolphin, April Maschmann, Keegan McGill, Mathew Moore, Drew Thompson, Kristy L Kounovsky-Shafer
To understand structural variation for personal genomics, an extensive ensemble of large DNA molecules will be required to span large structural variations. Nanocoding, a whole-genome analysis platform, can analyze large DNA molecules for the construction of physical restriction maps of entire genomes. However, handling of large DNA is difficult and a system is needed to concentrate large DNA molecules, while keeping the molecules intact. Insert technology was developed to protect large DNA molecules during routine cell lysis and molecular biology techniques...
October 26, 2018: Electrophoresis
Georgia M Parkin, Madhara Udawela, Andrew Gibbons, Brian Dean
Levels of a reference protein must be the same as a proportion of total protein in all tissues and, in the study of human diseases, cannot vary with factors such as age, gender or disease pathophysiology. It is increasingly apparent that there may be few, if any, proteins that display the characteristics of a reference protein within the human central nervous system (CNS). To begin to challenge this hypothesis, we used Western blotting to compare variance in levels of the "gold standard" reference protein, β-actin, in Brodmann's area 9 from 194 subjects to variance of total transferred protein measured as intensity of Ponceau S staining...
October 26, 2018: Electrophoresis
Michael C Breadmore, Wojciech Grochocki, Umme Kalsoom, Mónica N Alves, Sui Ching Phung, Masoomeh Tehrani Rokh, Joan M Cabot, Alireza Ghiasvand, Feng Li, Aliaa I Shallan, Aemi S Abdul Keyon, Ala A Alhusban, Hong Heng See, Alain Wuethrich, Mohamed Dawod, Joselito P Quirino
One of the most cited limitations of capillary and microchip electrophoresis is the poor sensitivity. This review continues to update this series of biannual reviews, first published in Electrophoresis in 2007, on developments in the field of online/in-line concentration methods in capillaries and microchips, covering the period July 2016-June 2018. It includes developments in the field of stacking, covering all methods from field-amplified sample stacking and large-volume sample stacking, through to isotachophoresis, dynamic pH junction, and sweeping...
October 26, 2018: Electrophoresis
Meltem Elitas, Yagmur Yildizhan, Monsur Islam, Rodrigo Martinez-Duarte, Didem Ozkazanc
Monocyte heterogeneity and its prevalence are revealed as indicator of several human diseases ranking from cardiovascular diseases to rheumatoid arthritis, chronic kidney diseases, autoimmune multiple sclerosis, and stroke injuries. When monocytes and macrophages are characterized and isolated with preserved genetic, phenotypic and functional properties, they can be used as label-free biomarkers for precise diagnostics and treatment of various diseases. Here, the dielectrophoretic responses of the monocytes and macrophages were examined...
October 26, 2018: Electrophoresis
Saikat Bhattacharjee, Debashis Roy, Anish Pal, Sirshendu De
Transport of salt through the wall of porous microtube is relevant in various physiological microcirculation systems. Transport phenomena based modeling of such system is undertaken in the present study considering a combined driving force consisting of pressure gradient and external electric field. Transport of salt is modeled in two domains, in the flow conduit and in the pores of porous wall of the microtube. The solute transport in the microtube is presented by convective-diffusive mass balance and it is solved using integral method under the framework of boundary layer analysis...
October 26, 2018: Electrophoresis
Fanyi Zhu, Mark A Hayes
Electrophoretic exclusion is a counterflow gradient technique that exploits hydrodynamic flow and electrophoretic forces to exclude, enrich, and separate analytes. Resolution for this technique has been theoretically examined and the smallest difference in electrophoretic mobilities that can be completely separated is estimated to be 10-13 cm2 /Vs. Traditional and mesoscale systems have been used, whereas microfluidics offers a greater range of geometries and configurations towards approaching this theoretical limit...
October 23, 2018: Electrophoresis
Liang Huang, Weihua He, Wenhui Wang
Cell rotation is widely required in various fields as an important technique for single cell manipulation. Usually, the electro-rotational manipulation of single cells by dielectrophoresis technologies requires at least three electrodes to generate rotating electric fields which induce cells to rotate. Here, we present a novel microfluidic chip capable of rotating single cell using only two planar electrodes by taking polarized cells as the extra electrodes with phase-shifted signal. To demonstrate this idea, we configured two parallel and planar electrodes as basic dielectrophoresis elements and placed trenches above these electrodes to attract cells, which were in turn polarized to be electrodes...
October 23, 2018: Electrophoresis
Chien-Hsuan Ko, Di Li, Amirreza Malekanfard, Yao-Nan Wang, Lung-Ming Fu, Xiangchun Xuan
Insulator-based dielectrophoresis (iDEP) has to date been almost entirely restricted to Newtonian fluids despite the fact that many of the chemical and biological fluids exhibit non-Newtonian characteristics. We present herein an experimental study of the fluid rheological effects on the electroosmotic flow of four types of polymer solutions, i.e., 2000 ppm xanthan gum (XG), 5% polyvinylpyrrolidone (PVP), 3000 ppm polyethylene oxide (PEO) and 200 ppm polyacrylamide (PAA) solutions, through a constriction microchannel under DC electric fields of up to 400 V/cm...
October 22, 2018: Electrophoresis
Chunye Liu, Xuejiao Zhang, Hui Jing, Jingshui Zhang, Yanqing Miao, Xifeng Zhai, Songyi Chen
An open-tubular capillary electrochromatography method has been developed for the determination of binding constants between β2 -adrenergic receptor (β2 -AR) and seven drugs. β2 -AR was oriented immobilized onto one part of inner surface of capillary via microwave-assisted technical synthesis. According to the linear relationship between coating length and the apparent mobility of analyte, the binding constant (Kb ) can be obtained by related theories and equations. The order of Kb values between drugs such as adrenaline hydrochloride, norepinephrine bitartrate, and propranolol hydrochloride with β2 -AR is well consistent with that reported in the literature...
October 16, 2018: Electrophoresis
Gui-Ping Zhu, Kian-Soo Ong, Karen Siew-Ling Chong, Jia-Feng Yao, Hu-Lin Huang, Fei Duan
Micro-structure patterned substrates attract our attention due to the special and programmable wettabilities. The interaction between the liquid and micro/nano structures gives rise to controllable spreading and thus evaporation. For exploration of the application versatility, the introduction of nanoparticles in liquid droplet results in interaction among particles, liquid and microstructures. In addition, temperature of the substrates strongly affects the spreading of the contact line and the evaporative property...
October 14, 2018: Electrophoresis
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