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Alternatives to Laboratory Animals: ATLA

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https://www.readbyqxmd.com/read/28094537/statistics-of-scientific-procedures-on-living-animals-great-britain-2015-highlighting-an-ongoing-upward-trend-in-animal-use-and-missed-opportunities
#1
Michelle Hudson-Shore
The Annual Statistics of Scientific Procedures on Living Animals Great Britain 2015 indicate that the Home Office were correct in recommending that caution should be exercised when interpreting the 2014 data as an apparent decline in animal experiments. The 2015 report shows that, as the changes to the format of the annual statistics have become more familiar and less problematic, there has been a re-emergence of the upward trend in animal research and testing in Great Britain. The 2015 statistics report an increase in animal procedures (up to 4,142,631) and in the number of animals used (up to 4,069,349)...
December 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/28094536/the-novel-induction-of-retinal-ganglion-cell-apoptosis-in-porcine-organ-culture-by-nmda-an-opportunity-for-the-replacement-of-animals-in-experiments
#2
Sandra Kuehn, Jose Hurst, Adelina Jashari, Kathrin Ahrens, Teresa Tsai, Ilan M Wunderlich, H Burkhard Dick, Stephanie C Joachim, Sven Schnichels
Some of the advantages of retina organ culture models include their efficient and easy handling and the ability to standardise relevant parameters. Additionally, when porcine eyes are obtained from the food industry, no animals are killed solely for research purposes. To induce retinal degeneration, a commonly used toxic substance, N-methyl-D-aspartate (NMDA), was applied to the cultures. To this end, organotypic cultures of porcine retinas were cultured and treated with different doses of NMDA (0 [control], 50, 100 and 200μM) on day 2 for 48 hours...
December 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/28094535/comparing-the-coral-and-random-forest-approaches-for-modelling-the-in-vitro-cytotoxicity-of-silica-nanomaterials
#3
Antonio Cassano, Richard L Marchese Robinson, Anna Palczewska, Tomasz Puzyn, Agnieszka Gajewicz, Lang Tran, Serena Manganelli, Mark T D Cronin
Nanotechnology is one of the most important technological developments of the 21st century. In silico methods to predict toxicity, such as quantitative structure-activity relationships (QSARs), promote the safe-by-design approach for the development of new materials, including nanomaterials. In this study, a set of cytotoxicity experimental data corresponding to 19 data points for silica nanomaterials were investigated, to compare the widely employed CORAL and Random Forest approaches in terms of their usefulness for developing so-called 'nano-QSAR' models...
December 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/28094534/in-vitro-assessment-of-skin-irritation-potential-of-surfactant-based-formulations-by-using-a-3-d-skin-reconstructed-tissue-model-and-cytokine-response
#4
Russel M Walters, Lisa Gandolfi, M Catherine Mack, Michael Fevola, Katharine Martin, Mathew T Hamilton, Allison Hilberer, Nicole Barnes, Nathan Wilt, Jennifer R Nash, Hans A Raabe, Gertrude-Emilia Costin
The personal care industry is focused on developing safe, more efficacious, and increasingly milder products, that are routinely undergoing preclinical and clinical testing before becoming available for consumer use on skin. In vitro systems based on skin reconstructed equivalents are now established for the preclinical assessment of product irritation potential and as alternative testing methods to the classic Draize rabbit skin irritation test. We have used the 3-D EpiDerm™ model system to evaluate tissue viability and primary cytokine interleukin-1α release as a way to evaluate the potential dermal irritation of 224 non-ionic, amphoteric and/or anionic surfactant-containing formulations, or individual raw materials...
December 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/28094533/animal-experimentation-the-statistics-speak-for-themselves
#5
EDITORIAL
Michael Balls, Robert Combes
No abstract text is available yet for this article.
December 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27805832/a-multi-faceted-approach-to-achieving-the-global-acceptance-of-animal-free-research-methods
#6
REVIEW
Jodie Melbourne, Patricia Bishop, Jeffrey Brown, Gilly Stoddart
In 2015, the PETA International Science Consortium Ltd. was awarded the Lush Training Prize for its broad approach to education and training on the effective use of human-relevant, non-animal research techniques. The prize was awarded for work that included hosting workshops and webinars, initiating in-person training sessions and developing educational resources. The Consortium works closely with industry and regulatory agencies to identify and overcome barriers to the validation and use of alternatives to animal testing, by using an approach that identifies, promotes and verifies the implementation of these methods...
October 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27805831/establishment-of-a-tumour-stroma-airway-model-oncocilair-to-accelerate-the-development-of-human-therapies-against-lung-cancer
#7
REVIEW
Christophe Mas, Bernadett Boda, Mireille Caul Futy, Song Huang, Ludovic Wisniewski, Samuel Constant
This paper highlights the work for which OncoTheis, a Swiss biotechnology company, engaged in the development of innovative bioengineered tissues and organoids for cancer research, was co-awarded the 2015 Lush Science Prize. Noting that the use of animal models failed to lead to the design of effective treatments for cancer, OncoTheis has opted to develop in vitro models based exclusively on human cells. The company currently focuses on lung cancer, which is the leading cause of cancer-related deaths worldwide, with more than one million deaths per year...
October 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27805830/body-on-a-chip-systems-for-animal-free-toxicity-testing
#8
REVIEW
Gretchen J Mahler, Mandy B Esch, Tracy Stokol, James J Hickman, Michael L Shuler
Body-on-a-chip systems replicate the size relationships of organs, blood distribution and blood flow, in accordance with human physiology. When operated with tissues derived from human cell sources, these systems are capable of simulating human metabolism, including the conversion of a prodrug to its effective metabolite, as well as its subsequent therapeutic actions and toxic side-effects. The system also permits the measurement of human tissue electrical and mechanical reactions, which provide a measure of functional response...
October 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27805829/2015-lush-science-prize
#9
REVIEW
Jenny McCann, Terry McCann
The Lush Prize supports animal-free testing by rewarding the most effective projects and individuals who have been working toward the goal of replacing animals in product or ingredient safety testing. Prizes are awarded for developments in five strategic areas: Science; Lobbying; Training; Public Awareness; and Young Researchers. Should there be a major breakthrough in 21st century toxicology, a Black Box Prize equivalent to the entire annual fund of £250,000 is awarded. A Background Paper is prepared each year, prior to the judging process, to provide the panel with a brief overview of current developments in the field of Replacement alternatives, particularly those relevant to the concept of toxicity pathways...
October 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27805828/the-adverse-outcome-pathway-for-skin-sensitisation-moving-closer-to-replacing-animal-testing
#10
REVIEW
Terry W Schultz, Gergana Dimitrova, Sabcho Dimitrov, Ovanes G Mekenyan
This article outlines the work of the Organisation for Economic Co-operation and Development (OECD) that led to being jointly awarded the 2015 Lush Black Box Prize. The award-winning work centred on the development of 'The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins'. This Adverse Outcome Pathway (AOP) has provided the mechanistic basis for the integration of skin sensitisation-related information. Recent developments in integrated approaches to testing and assessment, based on the AOP, are summarised...
October 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27805827/nrf2-activation-as-a-key-event-triggered-by-skin-sensitisers-the-development-of-the-stable-keratinosens-reporter-gene-assay
#11
REVIEW
Andreas Natsch, Roger Emter
The 21st century paradigm for toxicology and the adverse outcome pathway concept envisage a future toxicology largely based on mechanistic in vitro assays and relying mainly on cellular models. In the skin sensitisation field, this concept was not intuitive at the beginning. Given the high structural diversity of skin sensitising molecules, classical receptor binding as the molecular initiating event in a cell-based assay could be excluded from the start, leaving the question of how cells could sense potential skin sensitising chemicals and be able to differentiate them from non-sensitisers...
October 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27805826/the-use-of-peptide-reactivity-assays-for-skin-sensitisation-hazard-identification-and-risk-assessment
#12
REVIEW
G Frank Gerberick
Over the past 20 years or more, investigators have been developing non-animal test methods for use in assessing the skin sensitisation potential of chemicals. In parallel with this effort, the key biological events of skin sensitisation have been well-characterised in an Adverse Outcome Pathway (AOP) proposed by the Organisation for Economic Co-operation and Development (OECD). The key molecular initiating event of this AOP is haptenation or covalent modification of epidermal proteins. In this review, the strengths and limitations of the Direct Peptide Reactivity Assay (DPRA) are described, and the more recently developed Peroxidase Peptide Reactivity Assay (PPRA)...
October 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27805825/skin-sensitisation-adverse-outcome-pathways-and-alternatives
#13
REVIEW
David Basketter
For toxicologists who are in any way associated with skin sensitisation, the last two decades have seen a series of fundamental changes. We have migrated from old-style guinea-pig assays, via the refined and reduced Local Lymph Node Assay (LLNA), to witness the imminent dominance of in vitro and in silico methods. Yet, over the same period, the use of the output data for human safety assurance has evolved from 'black box' risk assessment, via the quantitative risk assessment enabled by the LLNA measurement of potency, to a new period of relative uncertainty...
October 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27805824/the-adverse-outcome-pathway-concept-a-basis-for-developing-regulatory-decision-making-tools
#14
REVIEW
Nathalie Delrue, Magdalini Sachana, Yuki Sakuratani, Anne Gourmelon, Eeva Leinala, Robert Diderich
The Adverse Outcome Pathway (AOP) concept is expected to guide risk assessors in their work to use all existing information on the effects of chemicals on humans and wildlife, and to target the generation of additional information to the regulatory objective. AOPs will therefore be used in the Organisation for Economic Co-operation and Development (OECD) chemical safety programme, as underlying scientific rationales for the development of alternative methods for hazard assessment, such as read-across, in vitro test methods or the development of integrated testing strategies that have the potential to replace animal tests...
October 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27805823/it-takes-a-village-stakeholder-participation-is-essential-to-transforming-science
#15
REVIEW
Kristie Sullivan
Efforts toward replacing the use of animals in toxicology testing have begun to make significant headway in the last several years, due to co-operative and pragmatic efforts on the part of many stakeholders, and the public pressure that non-governmental advocacy organisations represent. Science-focused advocacy organisations have a unique role to play in these efforts, as they often have flexibility to adapt quickly to keep a project going and forge connections among different kinds of stakeholders to help encourage buy-in...
October 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27805822/the-2015-lush-prize-awards
#16
EDITORIAL
Kelly A BéruBé, Craig Redmond
No abstract text is available yet for this article.
October 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27685189/acute-oral-toxicity-testing-scientific-evidence-and-practicability-should-govern-three-rs-activities
#17
Roland Buesen, Uwe Oberholz, Ursula G Sauer, Robert Landsiedel
Acute oral toxicity is determined for regulatory hazard classification or non-classification. The European Union Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) recommends the following modules for acute oral toxicity testing: a) the use of the in vitro 3T3 Neutral Red Uptake (NRU) test to identify substances not requiring classification and to estimate starting doses for in vivo acute oral toxicity studies; and b) the use of data from sub-acute toxicity studies to identify substances not requiring classification...
September 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27685188/the-european-citizens-stop-vivisection-initiative-and-the-revision-of-directive
#18
Andre Menache
Animal experimentation is presented to the public as an ongoing debate between research scientists on one hand, and the animal protection community on the other. An opportunity to break out of this mindset presented itself in the form of a European Citizens' Initiative, Stop Vivisection, which challenged Directive 2010/63/EU of the European Parliament and of the Council on the protection of animals for scientific purposes. The manifesto of the initiative called upon the European Commission to replace the existing Directive with a new proposal that does away with animal experimentation, and instead makes compulsory the use of human data as a predictive modality for the study of human diseases and responses to drugs...
September 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27685187/expectations-for-the-methodology-and-translation-of-animal-research-a-survey-of-the-general-public-medical-students-and-animal-researchers-in-north-america
#19
Ari R Joffe, Meredith Bara, Natalie Anton, Nathan Nobis
To determine what are considered acceptable standards for animal research (AR) methodology and translation rate to humans, a validated survey was sent to: a) a sample of the general public, via Sampling Survey International (SSI; Canada), Amazon Mechanical Turk (AMT; USA), a Canadian city festival (CF) and a Canadian children's hospital (CH); b) a sample of medical students (two first-year classes); and c) a sample of scientists (corresponding authors and academic paediatricians). There were 1379 responses from the general public sample (SSI, n = 557; AMT, n = 590; CF, n = 195; CH, n = 102), 205/330 (62%) medical student responses, and 23/323 (7%, too few to report) scientist responses...
September 2016: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/27685186/autologous-co-culture-of-primary-human-alveolar-macrophages-and-epithelial-cells-for-investigating-aerosol-medicines-part-ii-evaluation-of-il-10-loaded-microparticles-for-the-treatment-of-lung-inflammation
#20
Marius Hittinger, Nico Alexander Mell, Hanno Huwer, Brigitta Loretz, Nicole Schneider-Daum, Claus-Michael Lehr
Acute respiratory distress syndrome is linked to inflammatory processes in the human lung. The aim of this study was to mimic in vitro the treatment of lung inflammation by using a cell-based human autologous co-culture model. As a potential trial medication, we developed a pulmonary dry powder formulation loaded with interleukin-10 (IL-10), a potent anti-inflammatory cytokine. The inflammatory immune response was stimulated by lipopolysaccharide. The co-culture was combined with the Pharmaceutical Aerosol Deposition Device on Cell Cultures )PADDOCC), to deposit the IL-10-loaded microparticles on the inflamed co-culture model at the air-liquid interface...
September 2016: Alternatives to Laboratory Animals: ATLA
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