journal
MENU ▼
Read by QxMD icon Read
search

Molecular and Cellular Biology

journal
https://www.readbyqxmd.com/read/29038164/pka-creb-signaling-prevents-adriamycin-induced-podocyte-apoptosis-via-upregulation-of-mitochondrial-respiratory-chain-complexes
#1
Kewei Xie, Mingli Zhu, Peng Xiang, Xiaohuan Chen, Ayijiaken Kasimumali, Renhua Lu, Qin Wang, Shan Mou, Zhaohui Ni, Leyi Gu, Huihua Pang
Previous work showed that the activation of protein kinase A (PKA) signaling promoted mitochondrial fusion and prevented podocyte apoptosis. The cAMP response element binding protein (CREB) is the main downstream transcription factor of PKA signaling. Herein, we show that the pKA-agonist pCPT-cAMP prevented the production of adriamycin (ADR)-induced reactive oxygen species and apoptosis in podocytes, which were inhibited by CREB RNAi. The activation of PKA enhanced mitochondrial function and prevented the ADR-induced decrease of mitochondrial respiratory chain complex I subunits, NADH-ubiquinone oxidoreductase complex (ND) 1/3/4 genes, and protein expression...
October 16, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29038163/bcl11a-is-a-critical-component-of-a-transcriptional-network-that-activates-rag-expression-and-vdj-recombination
#2
Baeck-Seung Lee, Bum-Kyu Lee, Vishwanath R Iyer, Barry P Sleckman, Arthur L Shaffer, Gregory C Ippolito, Haley O Tucker, Joseph D Dekker
Recombination activating gene 1 (RAG1) and RAG2 are critical enzymes for initiating variable-diversity-joining (VDJ) segment recombination, an essential process for antigen receptor expression and lymphocyte development. The BCL11A transcription factor is required for B cell and Plasmacytoid dendritic cell (pDC) development, but its molecular function(s) in early B cell fate specification and commitment are unknown. We show here that the major B cell isoform, BCL11A-XL, binds directly to the RAG1 promoter as well as directly to regulatory regions of transcription factors previously implicated in both B cell and pDC development to activate RAG1 and RAG2 transcription in pro- and pre-B cells...
October 16, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29038162/systematic-analysis-of-human-cells-lacking-atg8-proteins-uncovers-roles-for-gabaraps-and-the-ccz1-mon1-regulator-c18orf8-rmc1-in-macro-and-selective-autophagic-flux
#3
Laura Pontano Vaites, Joao A Paulo, Edward L Huttlin, J Wade Harper
Selective and macro autophagy sequester specific organelles/substrates or bulk cytoplasm, respectively, inside autophagosomes as cargo for delivery to lysosomes. The mammalian ATG8 orthologues (MAP1LC3A/B/C and GABARAP/L1/L2) are ubiquitin (UB)-like proteins conjugated to the autophagosome membrane and are thought to facilitate cargo receptor recruitment, vesicle maturation, and lysosomal fusion. To elucidate the molecular functions of the ATG8 proteins, we engineered cells lacking genes for each subfamily as well as all six mammalian ATG8s...
October 16, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29038161/mechanistic-differences-in-transcription-initiation-at-tata-less-and-tata-containing-promoters
#4
Rafal Donczew, Steven Hahn
A yeast in vitro system was developed that is active for transcription at both TATA-containing and TATA-less promoters. Transcription with extracts made from cells depleted of TFIID subunit Taf1 demonstrated that promoters of both classes are TFIID-dependent, in agreement with recent in vivo findings. TFIID-depletion can be complemented in vitro by additional recombinant TBP at only the TATA-containing promoters. In contrast, high levels of TBP did not complement Taf1 depletion in vivo and instead repressed transcription from both promoter types...
October 16, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29038160/a-high-resolution-genome-wide-crispr-cas9-viability-screen-reveals-structural-features-and-contextual-diversity-of-the-human-cell-essential-proteome
#5
Thierry Bertomeu, Jasmin Coulombe-Huntington, Andrew Chatr-Aryamontri, Karine Bourdages, Etienne Coyaud, Brian Raught, Yu Xia, Mike Tyers
To interrogate genes essential for cell growth, proliferation and survival in human cells, we carried out a genome-wide CRISPR/Cas9 screen in a B-cell lymphoma line using a custom extended knockout (EKO) library of 278,754 sgRNAs that targeted 19,084 RefSeq genes, 20,852 alternatively-spliced exons and 3,872 hypothetical genes. A new statistical analysis tool called RANKS identified 2,280 essential genes, 234 of which were unique. Individual essential genes were validated experimentally and linked to ribosome biogenesis and stress responses...
October 16, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29038159/loss-of-erk1-2-in-the-retinal-pigment-epithelium-leads-to-rpe65-decrease-and-retinal-degeneration
#6
Aswin Pyakurel, Delphine Balmer, Marc K Saba-El-Leil, Caroline Kizilyaprak, Jean Daraspe, Bruno M Humbel, Laure Voisin, Yun Z Le, Johannes von Lintig, Sylvain Meloche, Raphaël Roduit
Recent work has suggested that the activity of extracellular signal-regulated kinase (ERK) 1/2 is increased in the retinal pigment epithelium (RPE) of age-related macular degeneration (ARMD) patients and therefore could be an attractive therapeutic target. Notably, ERK1/2 pathway inhibitors are used in cancer therapy, with severe and non-characterized ocular side effects. To decipher the role of ERK1/2 in RPE cells, we conditionally disrupted the Erk1 and Erk2 genes in the mouse RPE. Loss of ERK1/2 activity resulted in a significant decrease of RPE65 expression, a decrease in ocular retinoid levels concomitant with low visual function and a rapid disorganization of RPE cells, ultimately leading to retinal degeneration...
October 16, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28993481/mtor-bach2-cascade-controls-cell-cycle-and-class-switch-recombination-during-b-cell-differentiation
#7
Toru Tamahara, Kyoko Ochiai, Akihiko Muto, Yukinari Kato, Nicolas Sax, Mitsuyo Matsumoto, Takeyoshi Koseki, Kazuhiko Igarashi
The transcription factor Bach2 regulates both acquired and innate immunity at multiple steps including antibody class switching and regulatory T cell development in activated B and T cells, respectively. However, little is known about the molecular mechanisms of Bach2 regulation in response to signaling of cytokines and antigen. We show here that mammalian target of rapamycin (mTOR) controls Bach2 along B cell differentiation with two distinct mechanisms in pre-B cells. Firstly, mTOR complex 1 (mTORC1) inhibited accumulation of Bach2 protein in nuclei and reduced its stability...
October 9, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28993480/mitochondrial-abnormality-facilitates-cyst-formation-in-autosomal-dominant-polycystic-kidney-disease
#8
Yu Ishimoto, Reiko Inagi, Daisuke Yoshihara, Masanori Kugita, Shizuko Nagao, Akira Shimizu, Norihiko Takeda, Masaki Wake, Kenjiro Honda, Jing Zhou, Masaomi Nangaku
Autosomal dominant polycystic kidney disease (ADPKD) constitutes the most common inherited kidney disease. Mutations in the PKD1 and PKD2 genes, encoding respective polycystin-1 and polycystin-2 Ca(2+) ion channels, results in tubular epithelial cell-derived renal cysts. Recent clinical studies demonstrate oxidative stress as present early in ADPKD. Mitochondria comprise the primary reactive oxygen species source and also their main effector target; however, the pathophysiological role of mitochondria in ADPKD remains uncharacterized...
October 9, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28993479/lamin-b2-modulates-nucleolar-morphology-dynamics-and-function
#9
Ayantika Sen Gupta, Kundan Sengupta
The nucleolus is required for ribosome biogenesis. Human cells have 2-3 nucleoli associated with Nucleolar Organizer Region (NOR) bearing chromosomes. Increase in number and altered nucleolar morphology define cancer cells. However, the mechanisms that modulate nucleolar morphology and function are unclear. Here we show that in addition to the nuclear envelope, Lamin B2 localizes proximal to Nucleolin at the Granular Component (GC) of the nucleolus and associates with nucleolar proteins - Nucleolin and Nucleophosmin...
October 9, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28993478/the-effect-of-mutant-p53-proteins-on-glycolysis-and-mitochondrial-metabolism
#10
Matilda Eriksson, Gorbatchev Ambroise, Amanda Tomie Ouchida, Andre Lima Queiroz, Dominique Smith, Alfredo Gimenez-Cassina, Marcin P Iwanicki, Patricia A Muller, Erik Norberg, Helin Vakifahmetoglu-Norberg
TP53 is one of the most commonly mutated genes in human cancers. Unlike other tumor suppressors that are frequently deleted or acquire loss-of-function mutations, the majority of TP53 mutations in tumors are missense substitutions, which lead to the expression of full-length mutant proteins that accumulate in cancer cells and may confer unique gain-of-function (GOF) activities to promote tumorigenic events. Recently, mutant p53 proteins have been shown to mediate metabolic changes as a novel GOF to promote tumor development...
October 9, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28970327/genetic-separation-of-sae2-nuclease-activity-from-mre11-nuclease-functions-in-budding-yeast
#11
Sucheta Arora, Rajashree A Deshpande, Martin Budd, Judy Campbell, America Revere, Xiaoming Zhang, Kristina H Schmidt, Tanya T Paull
Sae2 promotes the repair of DNA double-strand breaks in Saccharomyces cerevisiae The role of Sae2 is linked to the Mre11/Rad50/Xrs2 (MRX) complex, which is important for the processing of DNA ends into single-stranded substrates for homologous recombination. Sae2 has intrinsic endonuclease activity, but the role of this activity has not been assessed independently from its functions in promoting Mre11 nuclease activity. Here we identify and characterize separation-of-function mutants that lack intrinsic nuclease activity or the ability to promote Mre11 endonucleolytic activity...
October 2, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28970326/heat-shock-protein-genes-undergo-dynamic-alteration-in-their-3d-structure-and-genomic-organization-in-response-to-thermal-stress
#12
Surabhi Chowdhary, Amoldeep S Kainth, David S Gross
Three-dimensional chromatin organization is important for proper gene regulation, yet how the genome is remodeled in response to stress is largely unknown. Here we use a highly sensitive version of chromosome conformation capture (3C) in combination with fluorescence microscopy to investigate Heat Shock Protein (HSP) gene conformation and 3D nuclear organization in budding yeast. In response to acute thermal stress, HSP genes undergo intense intragenic folding interactions that go well beyond 5' -3' gene looping previously described for Pol II genes...
October 2, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28923850/increasing-the-un-neddylated-cullin1-portion-rescues-the-csn-phenotypes-by-stabilizing-adaptor-modules-to-drive-scf-assembly
#13
Qingqing Liu, Yike Zhou, Ruiqi Tang, Xuehong Wang, Qiwen Hu, Ying Wang, Qun He
The dynamic SCF assembly is controlled by cycles of cullin neddylation/deneddylation based on the deneddylation activity of the COP9 signalosome (CSN) and global sequestration of cullins by CAND1. However, this prediction is hampered in recent studies, and the regulatory mechanism and key players remain to be identified. We found that, maintaining a proper Cul1(Nedd8)/Cul1 ratio is crucial to ensure SCF functions. Reducing the high Cul1(Nedd8)/Cul1 ratios in csn mutants through ectopic expression of the non-neddylatable Cul1(K722R) proteins or introducing the endogenous cul1(K722R) point mutation significantly rescues their defective phenotypes...
September 18, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28923849/bivalent-epigenetic-control-of-oncofetal-gene-expression-in-cancer
#14
Sayyed K Zaidi, Seth E Frietze, Jonathan A Gordon, Jessica L Heath, Terri Messier, Deli Hong, Joseph R Boyd, Mingu Kang, Anthony N Imbalzano, Jane B Lian, Janet L Stein, Gary S Stein
Multiple mechanisms of epigenetic control that include DNA methylation, histone modification, non-coding RNAs, and mitotic gene bookmarking play pivotal roles in stringent gene regulation during lineage commitment and maintenance. Experimental evidence indicates that bivalent chromatin domains, i.e., regions of genome that are marked by both H3K4me3 (activating) and H3K27me3 (repressive) histone modifications, are a key property of pluripotent stem cells. Bivalency of developmental genes during the G1 phase of the pluripotent stem cell cycle contributes to cell fate decisions...
September 18, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28894029/pari-regulates-stalled-replication-fork-processing-to-maintain-genome-stability-upon-replication-stress-in-mice
#15
Ayako L Mochizuki, Ami Katanaya, Eri Hayashi, Mihoko Hosokawa, Emiko Moribe, Akira Motegi, Masamichi Ishiai, Minoru Takata, Gen Kondoh, Hitomi Watanabe, Norio Nakatsuji, Shinichiro Chuma
DNA replication is frequently perturbed by intrinsic as well as extrinsic genotoxic stress. At damaged forks, DNA replication and repair activities require proper coordination to maintain the genome integrity. We show here that PARI anti-recombinase plays an essential role to modulate the initial response to replication stress in mice. PARI is functionally dormant at replisomes during normal replication, but upon replication stress, it enhances nascent strand shortening that is regulated by RAD51 and MRE11...
September 11, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28894028/mtorc1-regulates-both-general-autophagy-and-mitophagy-induction-after-oxidative-phosphorylation-uncoupling
#16
Alberto Bartolomé, Ana García-Aguilar, Shun-Ichiro Asahara, Yoshiaki Kido, Carlos Guillén, Utpal B Pajvani, Manuel Benito
The mechanistic target of rapamycin complex 1 (MTORC1) is a critical negative regulator of general autophagy. We hypothesized that MTORC1 may specifically regulate autophagic clearance of damaged mitochondria. To test this, we used cells lacking tuberous sclerosis complex 2 (TSC2 -/-), which show constitutive MTORC1 activation. TSC2 -/- cells show MTORC1-dependent impaired autophagic flux after chemical uncoupling of mitochondria, increased mitochondrial protein aging and accumulation of p62/SQSTM1 positive mitochondria...
September 11, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28894027/activation-status-coupled-transient-s-acylation-determines-membrane-partitioning-of-a-plant-rho-related-gtpase
#17
Nadav Sorek, Limor Poraty, Hasana Sternberg, Ella Buriakovsky, Einat Bar, Efraim Lewinsohn, Shaul Yalovsky
ROPs or RACs are plant Rho-related GTPases implicated in regulation of multitude of signaling pathways that function at the plasma membrane via posttranslational lipid modifications. The relations between ROP activation status and their membrane localization has not been established. Here, we show that endogenous ROPs, as well as a transgenic His6:GFP:AtROP6 fusion protein, were partitioned between Triton X-100 soluble and insoluble membranes. In contrast, the His6:GFP:Atrop6(CA) activated mutant accumulated exclusively in detergent resistant membranes...
September 11, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28874518/internal-associations-of-the-acidic-region-of-upstream-binding-factor-control-its-nucleolar-localization
#18
Shuhei Ueshima, Kyosuke Nagata, Mitsuru Okuwaki
Upstream binding factor (UBF) is a member of the high mobility group (HMG) box protein family, characterized by multiple HMG boxes and a C-terminal acidic region (AR). UBF is an essential transcription factor for ribosomal RNA genes and mediates the formation of transcriptionally active chromatin in the nucleolus. However, it remains unknown how UBF is specifically localized to the nucleolus. Here, we examined the molecular mechanisms that localize UBF to the nucleolus. We found that the first HMG box (HMG box 1), the linker region (LR), and the AR cooperatively regulate the nucleolar localization of UBF1...
September 5, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28847847/the-long-non-coding-rna-meg3-is-an-epigenetic-determinant-of-oncogenic-signaling-in-functional-pancreatic-neuroendocrine-tumor-cells
#19
Sucharitha Iyer, Sita D Modali, Sunita K Agarwal
The long non-coding RNA (lncRNA) MEG3 is significantly downregulated in pancreatic neuroendocrine tumors (PNETs). MEG3 loss corresponds with aberrant upregulation of the oncogenic HGF receptor c-MET in PNETs. Meg3 overexpression, in a mouse insulin-secreting PNET cell line, MIN6, downregulates c-Met expression. However, the molecular mechanism by which MEG3 regulates c-MET is not known. Using chromatin isolation by RNA purification and sequencing (ChIRP-Seq), we identified Meg3 binding to unique genomic regions, in and around the c-Met gene...
August 28, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28827316/rnf20-suppresses-tumorigenesis-by-inhibiting-srebp1c-pttg1-axis-in-kidney-cancer
#20
Jae Ho Lee, Yong Geun Jeon, Kyoung-Hwa Lee, Hye Won Lee, Jeu Park, Hagoon Jang, Minyong Kang, Hye Sun Lee, Hee Jin Cho, Do-Hyun Nam, Cheol Kwak, Jae Bum Kim
Elevated lipid metabolism promotes cancer cell proliferation. Clear cell renal cell carcinoma (ccRCC) is the most common subtype of kidney cancers, characterized by ectopic lipid accumulation. However, the relationship between aberrant lipid metabolism and tumorigenesis in ccRCC is not thoroughly understood. Here, we demonstrate that ring finger protein 20 (RNF20) acts as a tumor suppressor in ccRCC. RNF20 overexpression repressed lipogenesis and cell proliferation by inhibiting sterol regulatory element-binding protein 1c (SREBP1c), and SREBP1 suppression by either knockdown or the pharmacological inhibitor betulin attenuated proliferation and cell cycle progression in ccRCC cells...
August 21, 2017: Molecular and Cellular Biology
journal
journal
28205
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"