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Bioscience Reports

Camilla West, Michael McDermott
MicroRNAs are important cellular mediators of mRNA degradation and translation repression, which in turn can have an impact on various processes and, if their function is perturbed, can cause disease. Here, we summarise the recent manuscript by Zhong et al., which explores microRNA-146a and how it may play an indirect yet vital role in the proliferation of osteoarthritis (OA) chondrocytes. The data presented by the authors could have important implications for future OA therapies.
June 20, 2017: Bioscience Reports
Nianfeng Sun, Yu Xue, Ting Dai, Xiding Li, Nanxiang Zheng
Tripartite Motif Containing 25 (TRIM25) is a member of TRIM proteins and functions as an E3 ubiquitin ligase. It has been found to act as an oncogene in gastric cancer cells and abnormally expressed in cancers of female reproductive system. Here we investigated the function of TRIM25 in colorectal cancer. TRIM25 was found to be significantly up-regulated in colorectal cancer tissues and cancer cell lines through Real-time polymerase chain reaction assay. Colorectal cancer cells over-expressing TRIM25 exhibited a 2-fold higher proliferation and migration rate compared to their parental lines in vitro Moreover, TRIM25 also promoted tumor progression in vivo Further study indicated that TRIM25 worked through positively regulating transforming growth factor beta (TGF-β) signaling pathway to regulate the proliferation and invasion of colorectal cancer cells...
June 15, 2017: Bioscience Reports
Pei Li, Ruijie Zhang, Yuan Xu, Lei Song, Qiang Zhou
Nucleus pulposus (NP) cell senescence is a typical pathological feature within the degenerative intervertebral disc. As a potential inducing and aggregating factor of disc degeneration, mechanical overloading affects disc biology in multiple ways. This study was to investigate the NP cell senescence-associated phenotype under intermittent high compression in an ex vivo disc bioreactor culture, and the role of the p38-MAPK pathway in this regulatory process. Porcine discs were cultured in culture chambers of a self-developed mechanically active bioreactor and subjected to different magnitudes of dynamic compression (low-magnitude and high-magnitude: 0...
June 15, 2017: Bioscience Reports
Zhengzhang Li, Zhe Shen, Haoping Xue, Shi Cheng, Qun Ji, Yutan Liu, Xiangjun Yang
Atherosclerosis is a chronic inflammatory disease of the vascular wall. Dysfunction of cystic fibrosis transmembrane conductance regulator (CFTR) has been shown to result in inflammatory responses in cystic fibrosis (CF) patients. However, little is known about the role of CFTR in vascular inflammation and atherogenesis. Our results showed that CFTR was dominantly expressed in macrophages of atherosclerotic plaque and reduced in aorta and aortic sinus from atherosclerotic apolipoprotein E-deficient (apoE-/-) mice...
June 14, 2017: Bioscience Reports
Changli Wu, Rong Li, Haibing Luo, Mingfeng Xu, Xiujuan Zhang
The inhibitor CEP-33779 is a specific selective inhibitor of JAK2. In most somatic cells, JAK2 plays essential roles in cellular signal transduction and in the regulation of cell cycle. Little is known regarding the effects of JAK2 on mammalian oocyte maturation. In the present study, we investigated the effects of CEP-33779 on mouse oocytes meiosis and possible mechanisms of JAK2 during mouse oocyte maturation. We detected the distribution of JAK2 during mouse oocyte maturation. The results show that JAK2 was mainly distributed in the cytoplasm during maturation...
June 14, 2017: Bioscience Reports
Yudong Peng, Kai Meng, Lili Jiang, Yucheng Zhong, Yong Yang, Yin Lan, Qiuyang Zeng, Longxian Cheng
Endothelial cells (EC) injury is a major step for the pathological progression of atherosclerosis. Recent study demonstrated that thymic stromal lymphopoietin (TSLP) exerts a protective role in atherosclerosis. However, the effect of TSLP and the exact molecular mechanism involved in EC remains unknown. In the present study, we found that long noncoding RNA (lncRNA) HOTAIR was much lower in EC from atherosclerotic plaque. Functional assays showed that HOTAIR facilitated cell proliferation and migration, and suppressed apoptosis in EC...
June 14, 2017: Bioscience Reports
Kristian Matias Silander, Päivi Pihlajamaa, Biswajyoti Sahu, Olli Jänne, Leif C Andersson
We have investigated and characterized a novel ornithine decarboxylase-related protein (ODCrp) also annotated as gm853. ODCrp shows 41% amino acid sequence identity with ODC and 38% with ODC antizyme inhibitor 1 (AZIN1). The Odcrp gene is selectively expressed in the epithelium of proximal tubuli of mouse kidney with higher expression in males than in females. Like Odc in mouse kidney, Odcrp is also androgen-responsive with androgen receptor-binding loci within its regulatory region. ODCrp forms homodimers but does not heterodimerize with ODC...
June 12, 2017: Bioscience Reports
Juan Wang, Wen He, Xiao Xu, Liping Guo, Ying Zhang, Suxia Han, Difei Shen
Human coronary artery endothelial cells (HCAECs) have the potential to undergo fibrogenic endothelial mesenchymal transition (EndMT), which results in matrix-producing fibroblasts and thereby contributes to the pathogenesis of cardiac fibrosis. Recently, the profibrotic cytokine transforming growth factor-β (TGF-β) is shown to be the crucial pathogenic driver which has been verified to induce EndMT. C-Ski is an important regulator of TGF-β signaling. However, the detailed role of c-Ski and the molecular mechanisms by which c-Ski affects TGF-β-induced EndMT in HCAECs are not largely elucidated...
June 12, 2017: Bioscience Reports
Hongying Zhao, Jun Zhang, Haiyu Shao, Jianwen Liu, Mengran Jin, Jinping Chen, Yazeng Huang
Many miRNAs play critical roles in modulating various biological processes of osteoclast differentiation and function. Microphthalmia-associated transcription factor (MITF), a target of miR-340, served as pivotal transcription factor involved in osteoclast differentiation. However, the role of miR-340 and MITF during osteoclast differentiation has not yet been clearly established. Tartrate-resistant acid phosphatase (TRAP) staining assay was performed to identify osteoclasts differentiated from bone marrow-derived macrophages (BMMs)...
June 12, 2017: Bioscience Reports
Isabelle Tcholakov, Charles E Grimshaw, Lihong Shi, Andre Kiryanov, Sean T Murphy, Christopher J Larson, Artur Plonowski, Jacques Ermolieff
Prolyl Hydroxylases (PHDs) down-regulate the level of hypoxia-inducible factors (HIFs) by hydroxylating key proline residues which triggers the degradation of the protein and affect the cell and its ability to respond to hypoxic stress. Several small molecule PHD inhibitors are now in various preclinical and clinical stages for the treatment of anemia. This study provides a detail kinetic analysis for some of these inhibitors. The data generated in this study suggest that these compounds are reversible and compete directly with the co-substrate, 2-oxoglutarate (2-OG) for binding at the enzyme active site...
June 7, 2017: Bioscience Reports
Jing You, Hui Li, Xing-Ming Lu, Wei Li, Peng-Ye Wang, Shuo-Xing Dou, Xu-Guang Xi
G-quadruplexes are special structures existing at the ends of human telomeres, the folding kinetics of which are essential for their functions in, such as the maintenance of genome stability and the protection of chromosome ends. In this work, we investigated the folding kinetics of G-quadruplex in different monovalent cation environments and determined the detailed kinetic parameters for Na(+)- and K(+)-induced G-quadruplex folding and for its structural transition from the basket-type Na(+) form to the hybrid-type K(+) form...
June 6, 2017: Bioscience Reports
Sha Luan, Yue Gao, Chun Wang, Chun-Ying Pei, Chang-Jiu Zhao
This study aimed to investigate the correlation of CTLA-4 exon-1 rs231775A>G and promoter region rs5742909C>T polymorphisms with the therapeutic efficacy of radionuclide (131)I in Graves' disease amongst the Chinese Han population. The (131)I radionuclide therapy was applied to 261 patients with Graves' disease. Based on therapeutic efficacy, the patients were assigned into the remission and the non-remission groups. PCR-RFLP was applied to detect CTLA-4 exon-1 rs231775A>G and promoter region rs5742909C>T polymorphisms...
June 6, 2017: Bioscience Reports
Xinyi Zhang, Bo Liang, Xiaoyan Xu, Feifei Zhou, Lingyin Kong, Jingjing Shen, Yingying Xia, Liming Xuan, Yan Mao, Yongfeng Xue, Jichun Tan
With the development and clinical application of genomics, more and more concern is focused on single-cell sequencing. In the process of single-cell sequencing, whole genome amplification is a key step to enrich sample DNA. Previous studies have compared the performance of different WGA strategies on Illumina sequencing platforms, but there is no related research aimed at Ion Proton platform, which is also a popular next-generation sequencing platform. Here by amplifying cells from six cell lines with different karyotypes, we estimated the data features of four common commercial WGA kits (PicoPLEX WGA Kit, GenomePlex Single Cell Whole Genome Amplification Kit, MALBAC Single Cell Whole Genome Amplification Kit, and REPLI-g Single Cell Kit), including median absolute pairwise difference, uniformity, reproducibility, and fidelity, and examined their performance of copy number variation detection...
June 1, 2017: Bioscience Reports
John R Helliwell
Since the Protein Data Bank was founded in 1971 there are now over 120,000 depositions, the majority of which are from X-ray crystallography and 90% of those made use of synchrotron beamlines. At the Cambridge Structure Database, founded in 1965, there are more than 800,000 'small molecule' crystal structure depositions, and a very large number of those are relevant in the biosciences as ligands or cofactors. The technology for crystal structure analysis is still developing rapidly both at synchrotrons and in home labs...
June 1, 2017: Bioscience Reports
Olivier A Pierrat, Anju Paudyal, James Woodruff, Olga Koroleva, Samuel Boateng
The Exon Junction Complex (EJC) is the main mechanism by which cells select specific mRNAs for translation into protein. We hypothesised that the EJC is involved in the regulation of gene expression during the stress response in cardiac myocytes, with implications for the failing heart. In cultured rat neonatal myocytes, we examined the cellular distribution of two EJC components eIF4A3 and mago nashi homologue (Mago) in response to metabolic stress. There was significant relocalisation of eIF4A3 and Mago from the nucleus to cytoplasm following 18 hours of hypoxia...
May 31, 2017: Bioscience Reports
Yun-Ling Li, Yu-Fen Li, Hua-Feng Li, Huai-Qing Lv, De-Zhong Sun
The study aims to explore the role of stromal cell derived factor-1α (SDF-1α)/stromal cell derived factor receptor-4 (CXCR4) signaling pathway to the clinicopathological features and prognosis of patients with nasopharyngeal carcinoma (NPC). From January 2009 to December 2010, 102 patients with NPC and 80 patients with chronic nasopharyngitis were enrolled for the study. Immunohistochemical staining, qRT-PCR and Western blotting were employed to determine the expressions of SDF-1α and CXCR4 proteins in NPC tissues and chronic nasopharyngitis tissues...
May 30, 2017: Bioscience Reports
Qin Zhang, Shuxiang Zhang
Ovarian cancer is one of the leading causes of death among gynecological malignancies. Increasing evidences indicate that dysregulation of mi- croRNAs (miRNAs) play an important role in tumor radioresistance. The aim of this study is to investigate whether microRNA-214 (miR-214) was involved in radioresistance of human ovarian cancer. Here, we showed that miR-214 was significantly upregulated in ovarian cancer tissues and radi- oresistance ovarian cancer cell lines. Transfection of miR-214 agomir in radiosensitive ovarian cancer cell lines promoted them resistance to ioniz- ing radiation, whereas transfection of miR-214 antagomir in radiore- sistance ovarian cancer cell lines sensitized them to ionizing radiation again...
May 30, 2017: Bioscience Reports
Vinay Narwal, Neelam Yadav, Manisha Thakur, Chandra Shekhar Pundir
The nanoparticles (NPs) of hemoglobin (Hb) were prepared by desolvation method and characterized by transmission electron microscopy (TEM), ultraviolet (UV) spectroscopy and Fourier-transform infrared spectroscopy (FTIR). An amperometric H2O2 biosensor was constructed by immobilizing HbNPs covalently onto a polycrystalline Au electrode (AuE). HbNPs/AuE was characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectra (EIS) before and after immobilization of HbNPs...
May 26, 2017: Bioscience Reports
Feng Tian, Jian Xu, Fangxi Xue, Encui Guan, Xiaoguang Xu
Emerging evidences are accumulating that long noncoding RNAs (lncRNAs) have recently been identified to participate in various cellular processes. Terminal differentiation-induced ncRNA (TINCR) is a newly identified lncRNA with its functional roles not fully elucidated in human malignancy. The current study aims to identify the clinical significance of TINCR in prognosis and malignant progression of hepatocellular carcinoma (HCC). TINCR expression in HCC specimens with various stages of tumorigenesis were measured by qRT-PCR...
May 25, 2017: Bioscience Reports
Yuling Li, Shudong Chen, Zhengfei Shan, Liyan Bi, Shengqiang Yu, Yongwei Li, Sen Xu
We investigated the effect of miR-182-5p on the viability, proliferation, invasion and migration ability of human gastric cells by regulating the expression of RAB27A. Real-time PCR assay was used to detect the expression of miR-182-5 and RAB27A in human gastric carcinoma tissues, para-carcinoma tissues and different cell lines. Western blotting was also used to determine the RAB27A expression in both tissues and cell lines. We chose the HGC-27 cell line as experiment subject as it demonstrated the highest miR-182-5p level...
May 25, 2017: Bioscience Reports
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