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Bioscience Reports

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https://www.readbyqxmd.com/read/29162670/pu-1-deficient-mice-are-resistant-to-thioacetamide-induced-hepatic-fibrosis-pu-1-finely-regulates-sirt1-expression-via-transcriptional-promotion-of-mir-34a-29c-in-hscs
#1
Qing Liu, Yongming Zhang, Songzhu Yang, Yanfang Wu, Jiantao Wang, Weiwei Yu, Yanguo Liu
PU.1 is a critical transcription factor involving in many pathological processes. However, its exact role in activation of hepatic stellate cells (HSCs) and liver fibrosis was rarely reported. Here, we found that, in HSCs of PU.1(+/-) mice, Sirt1 mRNA expression was not changed but Sirt1 protein was significantly increased, suggesting its promoting role in Sirt1 translation. We then isolated HSCs from WT and PU.1(+/-) mice, and the pcDNA-PU.1 expression vector was transfected into PU.1(+/-) HSCs. We checked the levels of miR-34a and miR-29c, two Sirt1-targeting miRNAs, and protein levels of PU...
November 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29162669/non-labelled-lens-less-micro-endoscopic-approach-for-cellular-imaging-through-highly-scattering-media
#2
Omer Wagner, Aditya Pandya, Yoav Chemla, Hadar Pinhas, Irina Schelkanova, Asaf Shahmoon, Yossi Mandel, Alexandre Douplik, Zeev Zalevsky
We describe an imaging approach based on an optical setup made up of a miniature, lensless, minimally invasive endoscope scanning a sample and matching post processing techniques that enables enhanced imaging capabilities. The main scopes of this article are that this approach enables imaging beyond highly scattering medium and increases the resolution and signal to noise levels reaching single cell imaging. Our approach has more advantages over ordinary endoscope setups and other imaging techniques. It is not mechanically limited by a lens, the fiber can acquire images over long time periods (unlike current imaging methods such as OCT etc...
November 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29162668/huai-qi-huang-corrects-the-balance-of-th1-th2-and-treg-th17-in-an-ovalbumin-induced-asthma-mouse-model
#3
Peng Liang, Shao Peng, Man Zhang, Yingying Ma, Xinggang Zhen, Huijuan Li
This study is designed to determine whether Huai Qi Huang has immuno-regulatory effects on the Th1/Th2 and Treg/Th17 balance in ovalbumin (OVA)-induced asthma model mice. Asthma model mice were constructed by OVA treatment and Huai Qi Huang was administrated. The amount of migrated inflammatory cells in the bronchoalveolar lavage fluid (BALF) from the OVA mice was counted. The total immunoglobulin E (IgE) in the sera was detected by the IgE ELISA kit. Cell suspensions from the lung were stained with antibodies specific for CD4 and the master transcription factors for Th1 (T-bet), Th2 (Gata-3), Th17 (RORγt), and Treg (Foxp3)...
November 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29162667/reperfusion-of-occluded-branch-retinal-arteries-by-transluminal-nd-yag-laser-embolysis-combined-with-intravenous-thrombolysis-of-urokinase
#4
Fang Chai, Shanshuang Du, Xiquan Zhao, Runsheng Wang
Purpose.  To report successful treatment with transluminal Nd:YAG laser embolysis (TYE) combined with urokinase thrombolysis for reperfusion of occluded branch retinal arteries with visible emboli. Methods A total of 34 eyes from 34 patients with acute, severe vision loss secondary to a branch retinal artery occlusion with visible emboli and retinal whitening were examined. Each patient was administered TYE therapy, which focused on the embolus, using an ocular contact lens; a 0.3-0.9 mJ laser pulse was delivered directly and gradually according to the reaction...
November 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29162666/molecular-characterization-and-expression-analysis-of-cs%C3%AE-%C3%AE-defensin-genes-from-the-scorpion-mesobuthus-martensii
#5
Yange Lang, Xiaohuan Pi, Zhiyong Di, Quian Zhang, Huijuan Wang, Bingzheng Shen, Fangfang Li, Gaomin Liu, Yao Yu, Xuan Li, Yingliang Wu, Wenxin Li, Zhijian Cao
Defensins are important components of innate host defence system against bacteria, fungi, parasites and viruses. Here, we predicted six potential defensin genes from the genome of the scorpion Mesobuthus martensii and then validated four genes of them via the combination of PCR and genomic sequence analysis. These four scorpion defensin genes share the same gene organization and structure of two exons and one phase-I intron with the GT-AG rule. Conserved motif and phylogenetic analysis showed that they belong to the members of the invertebrate CSαβ defensin family...
November 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29162665/the-beneficial-effects-of-curcumin-in-cirrhotic-rats-with-portal-hypertension
#6
Shao-Jung Hsu, Jing-Yi Lee, Te-Yueh Lin, Yu-Hsin Hsieh, Hui-Chun Huang, Fa-Yauh Lee, Han-Chieh Lin, Ming-Chih Hou, Shou-Dong Lee
In liver cirrhosis with portal hypertension, the uneven distribution of vasoactive substances leads to increased intrahepatic vascular resistance and splanchnic vasodilatation. Angiogenesis also induces increased portal inflow and portosystemic collaterals. The collaterals may induce lethal complications such as gastroesophageal variceal hemorrhage, but the therapeutic effect of vasoconstrictors is still suboptimal due to poor collateral vasoresponsivenss. Curcumin has aroused much attention for its anti-fibrosis, vasoactive and anti-angiogenesis actions...
November 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29138264/comprehensive-investigation-of-cytokine-and-immune-related-gene-variants-in-hbv-associated-hepatocellular-carcinoma-patients
#7
Fengxue Yu, Xiaolin Zhang, Suzhai Tian, Lianxia Geng, Weili Xu, Ning Ma, Mingbang Wang, Yuan Jia, Xuechen Liu, Junji Ma, Yuan Quan, Chaojun Zhang, Lina Guo, Wenting An, Dianwu Liu
Background & Aims: Host genotype may be closely related to the different outcomes of Hepatitis B virus (HBV) infection. To identify the association of variants and HBV infection, we comprehensively investigated the cytokine and immune related gene mutations in patients with HBV associated hepatocellular carcinoma (HBV-HCC). Methods: Fifty-three HBV-HCC patients, 53 self-healing cases (SH) with HBV infection history and 53 healthy controls (HC) were recruited, the whole exon region of 404 genes were sequenced at > 900x depth...
November 14, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29138263/srebp-1c-as-a-molecular-bridge-between-lipogenesis-and-cell-cycle-progression-of-clear-cell-renal-carcinoma
#8
Gautam Sethi, Muthu K Shanmugam, Alan Prem Kumar
SREBP-1c promotes lipogenesis and tumor growth in various cancers. It is well-known that clear cell renal cell carcinoma (ccRCC), a major subtype of the kidney cancers, exhibits elevated lipid accumulation. However, it has not been fully understood how lipid metabolism might be associated with cell cycle regulation in ccRCC. In recent issue of Molecular and Cellular Biology , Lee et al. demonstrate that SREBP-1c is upregulated in ccRCC by RNF20 downregulation, leading to aberrant lipid storage and PTTG1-dependent cell cycle progression...
November 14, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29133459/pathophysiological-changes-of-the-cerebellum-and-brain-stem-in-a-rabbit-model-after-superior-petrosal-vein-sacrifice
#9
Lei Cheng, Pin Guo, Yi-Wei Liao, Hong-Liang Zhang, Huan-Ting Li, Xianrui Yuan
In certain surgical procedures sacrifice of the superior petrosal vein (SPV) is required. Previous studies have reported transient cerebellar edema, venous infarction or hemorrhage might occur after sectioning of the SPV. This study investigated the pathophysiological changes of cerebellum and brain stem after SPV sacrifice. Rabbits were divided into the operation group where the SPV was sacrificed and the control group where the SPV remained intact. Each group was further subdivided into 4, 8, 12, 24, 48 and 72 hours groups which represented the time period from sacrifice of the SPV to sacrifice of the rabbits...
November 13, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29127105/epor2-%C3%A3-cr2-independendent-effects-of-low-dose-epoetin-a-in-porcine-liver-transplantation
#10
Linus Kebschull, Leon Franz Christoph Theilmann, Annika Mohr, Wencke Uennigmann, Sandra Stoeppeler, Barbara Heitplatz, Hans-Ullrich Spiegel, Ralf Bahde, Daniel Michael Palmes, Felix Becker
Ischemia-reperfusion injury (IRI) remains the key component of graft damage during transplantation. Erythropoietin (EPO) induces anti-inflammatory and anti-apoptotic effects via the EPOR2/ßcR2-complex, with the potential risk of thrombosis. Previous work indicates that EPO has EPOR2/ßcR2-independent protective effects via direct effects on the endothelium. As the EPOR2/ßcR2-receptor has a very low affinity for EPO, we aimed to test the hypothesis that EPO doses below the level that stimulate this receptor elicit cytoprotective effects via endothelial stimulation in a porcine liver transplantation model...
November 10, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29127104/chemical-suppressors-of-mlo-mediated-powdery-mildew-resistance
#11
Hongpo Wu, Mark Kwaaitaal, Roxana Strugala, Ulrich Schaffrath, Pawel Bednarek, Ralph Panstruga
Loss of function of barley Mlo confers durable broad-spectrum penetration resistance to the barley powdery mildew pathogen , Blumeria graminis f. sp. hordei ( Bgh ). Given the importance of mlo mutants in agriculture, surprisingly few molecular components have been identified to be required for this type of resistance in barley. With the aim to identify novel cellular factors contributing to mlo -based resistance, we devised a pharmacological inhibitor screen. Of the 41 rationally chosen compounds tested, five caused a partial suppression of mlo resistance in barley, indicated by increased levels of Bgh host cell entry...
November 10, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29101242/busulfan-administration-produces-toxic-effects-on-epididymal-morphology-and-inhibits-the-expression-of-zo-1-and-vimentin-in-the-mouse-epididymis
#12
Fang Fang, Ke Ni, Yiting Cai, Qian Zhao, Jin Shang, Xiaoke Zhang, Shiliang Shen, Chengliang Xiong
Busulfan is an alkane sulfonate currently used as an anticancer drug and to prepare azoospermic animal models, because it selectively destroys differentiated spermatogonia in the testes. However, few studies have focused on the exact effects of busulfan treatment on the epididymis currently. This study assessed the effect of busulfan on epididymal morphology and the blood-epididymis barrier in mice. We treated mice with a single injection of busulfan and detected the effect at different time points. We showed that busulfan was toxic to the morphological structure and function of the epididymis...
November 3, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29101241/upregulation-of-long-non-coding-rna-snhg20-promotes-ovarian-cancer-progression-via-wnt-%C3%AE-catenin-signaling
#13
Shanyang He, Yunhe Zhao, Xiaoping Wang, Yalan Deng, Zhiyong Wan, Shuzhong Yao, Hongwei Shen
Long non-coding RNA SNHG20 has been demonstrated as playing crucial regulatory roles in a many types of cancer. However, the biological function of lncRNA SNHG20 in ovarian cancer are still unclear. In this study, we found that lncRNA SNHG20 was significantly increased in ovarian cancer. In addition, lncRNA SNHG20 knockdown suppressed the ovarian cancer progression, whereas overexpression of SNHG20 showed the opposite effects. Moreover, our results also revealed that lncRNA SNHG20 knockdown inhibited Wnt/β-catenin signaling activity by suppressing β-catenin expression and reversing the downstream target gene expression...
November 3, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29101240/ambra1-induces-autophagy-and-desensitizes-human-prostate-cancer-cells-to-cisplatin
#14
Jie Liu, Zhiyuan Chen, Jia Guo, Lei Wang, Xiuheng Liu
Prostate cancer (PCa), the second most mortal cancer from developed countries, presents a high level of chemo-resistance. There is emerging evidence underscores the critical role of autophagy in the onset, progression and chemo-resistance of PCa. In the present study, we investigated the possible role of a novel autophagy regulator, Activating Molecule in Beclin1-Regulated Autophagy1 (Ambra1), a novel ATG gene in the sensitivity or PCa cells to cisplatin. We explored the regulation by the Ambra1 manipulation on the induction of apoptosis and autophagy in human PCa DU145 cells in the presence of cisplatin, via up- or down-regulating Ambra1 expression...
November 3, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29097484/uric-acid-promotes-oxidative-stress-and-enhances-vascular-endothelial-cell-apoptosis-in-rats-with-middle-cerebral-artery-occlusion
#15
Chengfu Song, Xiangdong Zhao
In patients with cerebral infarction (CI), elevated serum uric acid (UA) level may exacerbate the occurrence and development of carotid atherosclerosis  (AS). Our study intended to explore the underlying mechanism. We enrolled 86 patients with CI, and divided them into 4 groups: Non-AS, AS- mild, AS- moderate and AS-severe groups, the levels of UA and oxidative stress - related factors in serum were detected. The middle cerebral artery occlusion (MCAO) model was used to stimulate CI in rats, and different doses of UA were administrated...
November 2, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29097483/the-relationship-between-systemic-iron-homeostasis-and-erythropoiesis
#16
Gautam Rishi, V Nathan Subramaniam
Red blood cell production (erythropoiesis) is the single largest consumer of iron in the body; this need is satisfied by maintaining a sensitive regulation of iron levels. The level of erythropoietic demand regulates the expression of the iron hormone-hepcidin and thus iron absorption. Erythropoiesis-mediated regulation of hepcidin is an area of increasing importance and recent studies have identified a number of potential regulatory proteins. This review summarizes our current knowledge about these candidate erythroid regulators of hepcidin and the relation between transferrin receptors and erythropoiesis...
November 2, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29089469/the-association-between-cd28-gene-rs3116496-polymorphism-and-breast-cancer-risk-in-chinese-women
#17
Yuxiang Yan, Xianbo Zhang
T lymphocyte activation plays an important role in suppressing the development of human cancers including breast cancer (BC). Cluster of differentiation 28 (CD28) is the primary T-cell co-stimulatory molecule and enhances T-cell activation and proliferation. To examine the role of CD28 gene polymorphism in BC, we conducted a case-control study involving 312 BC patients and 312 controls in a Chinese Han population. Bioinformatics analyses were conducted to analyze the expression level of CD28 and its association with overall survival (OS) of BC...
October 31, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29089468/protective-effect-of-htk-solution-on-postoperative-pulmonary-function-in-infants-with-chd-and-pah
#18
Jindong Li, Yanhong Wu, Xudong Tian, Jiantang Wang, Mingfeng Dong, Anbiao Wang
Objective In this study, we aimed to investigate the effect of pulmonary arterial perfusion (PAP) with HTK on lung protection in infants with congenital heart disease (CHD) and pulmonary arterial hypertension (PAH) after cardiopulmonary bypass (CPB). Methods Fifty infant patients with CHD and PAH at our hospital from January 2016 to February 2017 were randomly divided into control group and HTK group. The level of IL-6, MDA and ET-1 in serum were detected using ELISA Kit. Oxygen index (OI) and respiratory index (RI) were calculated at each time point...
October 31, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29089467/suppression-of-stim1-reduced-intracellular-calcium-concentration-and-attenuated-hypoxia-reoxygenation-induced-apoptosis-in-h9c2-cells
#19
Fei He, Qianfu Wu, Banglong Xu, Xiaocheng Wang, Jixiong Wu, Li Huang, Jing Cheng
Objective: Previous studies have demonstrated STIM1-mediated store-operated Ca(2+) entry(SOCE) contributes to intracellular Ca(2+) accumulation. The present study aimed to investigate the expression of STIM1 and Orai1/TRPC1 in the context of myocardial ischemia/reperfusion injury(MIRI) and the effect of STIM1 inhibition on Ca(2+) accumulation and apoptosis in H9c2 cardiomyocytes subjected to hypoxia/reoxygenation(H/R). Methods: Expression of STIM1/Orai1/TRPC1 was determined by RT-PCR and Western Blot in mice subjected to MIRI and H9C2 cardiomyocytes subjected to H/R...
October 31, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29089466/preclinical-and-clinical-advances-in-transposon-based-gene-therapy
#20
Jaitip Tipanee, Yoke Chin Chai, Thierry VandenDriessche, Marinee K Chuah
Transposons derived from Sleeping Beauty (SB), piggyBac (PB) or Tol2 typically require cotransfection of transposon DNA with a transposase either as an expression plasmid or mRNA. Consequently, this results in genomic integration of the potentially therapeutic gene into chromosomes of the desired target cells and thus conferring stable expression. Nonviral transfection methods are typically preferred to deliver the transposon components in to the target cells. However, these methods do not match the efficacy typically attained with viral vectors and are sometimes associated with cellular toxicity evoked by the DNA itself...
October 31, 2017: Bioscience Reports
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