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Bioscience Reports

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https://www.readbyqxmd.com/read/27913753/neutrophil-derived-microparticles-are-released-into-the-coronary-circulation-following-percutaneous-coronary-intervention-in-acute-coronary-syndromes
#1
Gonzalo J Martinez, Jennifer Y Barraclough, Shirley Nakhla, Vivian Kienzle, Stacy Robertson, Ziad Mallat, David S Celermajer, Sanjay Patel
AIMS: To evaluate (i) local coronary and systemic levels of microparticles (MP) in Acute Coronary Syndrome (ACS) and Stable Angina Pectoris (SAP) patients and (ii) their release after plaque disruption with percutaneous coronary intervention (PCI). BACKGROUND: Microparticles are small vesicles originating from plasma membranes of cells after activation or apoptosis and are implicated in the pathogenesis of atherosclerosis. Neutrophils play a role in plaque destabilisation and shed neutrophil-derived microparticles have the potential to drive significant pro-inflammatory and thrombotic downstream effects...
December 2, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27913752/expression-and-bioactivity-of-human-alpha-fetoprotein-in-a-bac-to-bac-system
#2
Bo Lin, Kun Liu, Wenting Wang, Wei Li, Xu Dong, Yi Chen, Yan Lu, Junli Guo, Mingyue Zhu, Mengsen Li
Alpha-fetoprotein (AFP) is an early serum growth factor in fetal embryonic development and hepatic oncogenesis. A growing number of investigations of AFP as a tumor-specific biomarker have concluded that AFP is an important target for cancer treatment. AFP also plays an immunomodulatory role in the treatment of several autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, myasthenia gravis, and thyroiditis. In an effort to support biochemical screening and drug design and discovery, we attempted to express and purify human AFP in a Bac-to-Bac system...
December 2, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27913751/new-fungal-defensin-like-peptides-provide-evidence-for-fold-change-of-proteins-in-evolution
#3
Yucheng Wu, Bin Gao, Shunyi Zhu
Defensins containing a consensus cystine framework, Cys[1]..Cys[2]X3Cys[3]..Cys[4]..Cys[5]X1Cys[6] ("X", any amino acids normally except Cys; "...", variable residue numbers), are extensively distributed in a variety of multicellular organisms (plants, fungi and invertebrates) and essentially involved in immunity as microbicidal agents. This framework is a prerequisite for forming a cystine-stabilized α-helix and β-sheet (CSαβ) fold, in which the two invariant motifs, Cys[2]X3Cys[3]/Cys[5]X1Cys[6], are key determinants of fold formation...
December 2, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27899435/imaging-oxygen-metabolism-with-hyperpolarized-magnetic-resonance-a-novel-approach-for-cardiac-and-renal-function%C3%A2-assessement
#4
Marie Schroeder, Christoffer Lausten
Every tissue in the body critically depends on meeting its energetic demands with sufficient oxygen supply. Oxygen supply/demand imbalances underlie the diseases that inflict the greatest socioeconomic burden globally. The purpose of this review is to examine how hyperpolarized contrast media, used in combination with MR data acquisition methods, may advance our ability to assess oxygen metabolism non-invasively and thus improve management of clinical disease. We first introduce the concept of hyperpolarization and how hyperpolarized contrast media have been practically implemented to achieve translational and clinical research...
November 29, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27884978/estrogen-receptor-negativity-in-breast-cancer-a-cause-or-consequence
#5
Vijaya Narasihma Reddy Gajulapalli, Vijaya Lakshmi Malisetty, Suresh Kumar Chitta, Bramanandam Manavathi
Endocrine resistance, which occurs either by de novo or acquired route, is posing a major challenge in treating hormone-dependent breast cancers by endocrine therapies. The loss of ERα expression is the vital cause of establishing endocrine resistance in this subtype. Understanding the mechanisms that determine the causes of this phenomenon are therefore essential to reduce the disease efficacy. But how we negate estrogen receptor (ER) negativity and endocrine resistance in breast cancer is questionable, to answer that two important approaches are considered: 1) Understanding the cellular origin of heterogeneity and ER negativity in breast cancers, and 2) characterization of molecular regulators of endocrine resistance...
November 24, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27884977/nanog-regulates-epithelial-mesenchymal-transition-and-chemoresistance-in-ovarian-cancer
#6
Shan Qin, Yanfang Li, Xuexia Cao, Jiexian Du, Xianghua Huang
A key transcription factor associated with poor prognosis and resistance to chemotherapy in ovarian cancer is NANOG. However, the mechanism by which NANOG functions remains undefined. It has been suggested that epithelial to mesenchymal transition (EMT) also contributes to development of drug resistance in different cancers. We thus determined whether NANOG expression was associated with EMT and chemoresistance in epithelial ovarian cancer cells. NANOG expression was increased in epithelial ovarian cancer cell lines compared with its expression in normal epithelial ovarian cell lines...
November 24, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27879456/hindlimb-spasticity-after-unilateral-motor-cortex-lesion-in-rats-is-reduced-by-contralateral-nerve-root-transfer
#7
Haiyang Zong, Fenfen Ma, Laiyin Zhang, Huiping Lu, Jingru Gong, Min Cai, Haodong Lin, Yizhun Zhu, Chunlin Hou
The aim was to test the feasibility and effectiveness of contralateral nerve root transfer in reducing post stroke spasticity of the affected hindlimb muscles in rats. In our study, we for the first time created a novel animal hindlimb spastic hemiplegia model in rats with photothrombotic lesion of unilateral motor cortex and we established a novel surgical procedure in reducing motor cortex lesion induced hindlimb spastic hemiplegia in rats. Thirty six rats were randomized into three groups. In group A, rats received sham operation...
November 22, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27852797/modulation-of-mir-146a-complement-factor-h-mediated-inflammatory-responses-in-a-rat-model-of-temporal-lobe-epilepsy
#8
Fang He, Bei Liu, Qiang Meng, Yang Sun, Weiwen Wang, Chao Wang
Increasing evidence supports the involvement of inflammatory and immune processes in temporal lobe epilepsy (TLE). MicroRNAs (miRNA) represent small regulatory RNA molecules that have been shown to act as negative regulators of gene expression controlling different biological processes, including immune-system homeostasis and function. We investigated the expression and cellular distribution of miRNA-146a (miR-146a) in a rat model of TLE. Prominent upregulation of miR-146a activation was evident at 1 week after status epilepticus (SE) and persisted in the chronic phase...
November 16, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27836981/a-computational-fluid-structure-interaction-model-to-predict-the-biomechanical-properties-of-the-artificial-functionally-graded-aorta
#9
Arezoo Khosravi, Milad Salimi Bani, Hossein Bahreinizad, Alireza Karimi
In this study, three layers of the ascending aorta in respect to the time and space at various blood pressures have been simulated. Two well-known commercial Finite Element (FE) software have used to be able to provide a range of reliable numerical results while independent on the software type. The radial displacement versus the time as well as the peripheral stress and von Misses stress of the aorta have calculated. The aorta model was validated using the Differential Quadrature Method (DQM) solution and, then, in order to design Functionally Graded Materials (FGMs) with different heterogeneous indexes for the artificial vessel, two different materials have been employed...
November 11, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27834666/proliferation-in-culture-of-primordial-germ-cells-derived-from-embryonic-stem-cell-induction-by-retinoic-acid
#10
Zohreh Makoolati, Mansoureh Movahedin, Mehdi Forouzandeh-Moghadam
An in vitro system that supports primordial germ cells (PGCs) survival and proliferation is useful for enhancement of these cells and efficient transplantation in infertility disorders. One approach is cultivation of PGCs under proper conditions that allow self-renewal and proliferation of PGCs. For this purpose, we compared the effects of different concentrations of retinoic acid (RA), and the effect of PGCs co-culture with STO cells on the proliferation of embryonic stem cells (ESCs)- derived PGCs. One-day-old embryoid body (EB) was cultured for 4 days in simple culture system at the presence of 5 ng/ml BMP4 (SCB group) for PGC induction...
November 10, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27815507/suppression-of-neointima-formation-by-targeting-%C3%AE-catenin-tcf-pathway
#11
Helen Williams, Sadie Slater, Sarah Jane George
Coronary artery disease is treated by vein grafting and stent implantation.  Late vein graft failure and restenosis of stented arteries reduces the success rates of these approaches and is caused by neointima formation. We have previously shown that Wnt proteins are upregulated during intimal thickening, and have speculated that these lead to activation of downstream genes with β-catenin/T-cell factor (TCF)-responsive promoters. In this study we aimed to provide evidence that β-catenin/TCF signalling promotes neointima formation and assess whether targeting this pathway has potential for reducing neointima formation...
November 4, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27811014/regulated-necrosis-related-molecule-mrna-expression-in-humans-mice-and-murine-acute-tissue-injury-systemic-autoimmunity-leading-to-progressive-organ-damage-and-progressive-fibrosis
#12
Mohsen Honarpisheh, Jyaysi Desai, Julian A Marschner, Marc Weidenbusch, Maciej Lech, Volker Vielhauer, Hans-Joachim Anders, Shrikant R Mulay
The species-specific, as well as organ-specific expression of regulated necrosis (RN)-related molecules, is not known. We determined the expression levels of TNFR1, RIPK1, RIPK3, MLKL, CASP8, FADD, CIAP1, CIAP2, GPX4, CYPD, CASP1, NLRP3, and PARP1 in human and mouse solid organs. We observed significant differences in expression of these molecules between human and mice. In addition, we characterized their expression profiles in acute as well as persistent tissue injury and chronic tissue remodeling using acute and chronic kidney injury models...
November 3, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27811013/characteristics-of-a-thyroid-carcinoma-cell-line-derived-from-spinal-metastasis
#13
Zhenhua Zhou, Yan Li, Xu Yan, Xudong Wang, Su Chen, Jianru Xiao
A thyroid carcinoma cell line named THY28 was established through primary culture of the surgical specimens, which were derived from a Chinese patient with spinal metastasis. The cell morphology, growth kinetics, cell cycle, chromosome number, cell capability of migration, tumorigenicity, cytogenetic features of the cell line were investigated. THY28 cells were  subcultured in vitro for more than 50 passages with a human karyotype. The modal number of its chromosomes was mainly from 67 to 85. The doubling time of THY28 cells was 56 hours...
November 3, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27803125/efficient-and-simple-approach-to-in-vitro-culture-of-primary-epithelial-cancer-cells
#14
Karolina Janik, Marta Popeda, Joanna Peciak, Kamila Rosiak, Maciej Smolarz, Cezary Treda, Piotr Rieske, Ewelina Stoczynska-Fidelus, Magdalena Ksiazkiewicz
Primary cancer cells constitute a favorable testing platform for in vitro research in oncology field as they reflect tumour state more accurately than the most commonly employed stable cell lines. Unfortunately, due to limited availability of material and difficulties with protocols validation, primary models are rarely implemented into laboratory practice. We have compared protocols for primary cultures, differing in media components and plate coatings. In terms of culture establishment, application of Geltrex® coating demonstrated equal efficiency to feeder layer (83 vs...
November 1, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27789781/the-dnae-polymerase-from-deinococcus-radiodurans-features-reca-dependent-dna-polymerase-activity
#15
Lorenzo Randi, Alessandro Perrone, Mirko Maturi, Fabrizio Dal Piaz, Michela Camerani, Alejandro Hochkoeppler
We report here on the catalytic properties of the Deinococcus radiodurans DNA Polymerase III α subunit (αDr). The αDr enzyme was overexpressed in E. coli , both in soluble form and as inclusion bodies. When purified from soluble protein extracts, αDr was found to be tightly associated with E. coli RNA Polymerase, from which αDr could not be dissociated. On the contrary, when refolded from inclusion bodies, αDr was devoid of E. coli RNA Polymerase, and was purified to homogeneity. When assayed with different DNA substrates, αDr featured slower DNA extension rates when compared to the corresponding enzyme from E...
October 27, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27789780/influence-of-heme-environment-on-the-catalytic-properties-of-the-tetrathionate-reductase-tsda-from-campylobacter-jejuni
#16
Julia M Kurth, Julea N Butt, David J Kelly, Christiane Dahl
Bifunctional diheme cytochrome c thiosul-fate dehydrogenases/tetrathionate reductases (TsdA) exhibit different catalytic properties depend-ing on the source organism. In the human food-borne intestinal pathogen Campylobacter jejuni , TsdA functions as a tetrathionate reductase en-abling respiration with tetrathionate as an al-ternative electron acceptor. Here, evidence is provided that Cys(138) and Met(255) serve as the sixth ligands of Heme 1 and Heme 2, respec-tively, in the oxidized Cj TsdA wt protein. Re-placement of Cys(138) resulted in a virtually inac-tive enzyme, confirming Heme 1 as the active site heme...
October 27, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27780892/diabetes-negatively-affects-cortical-and-striatal-gabaergic-neurons-an-effect-that-is-partially-counteracted-by-exendin-4
#17
Martin Larsson, Grazyna Lietzau, David Nathanson, Claes-Göran Östenson, Carina Mallard, Maria E Johansson, Thomas Nyström, Cesare Patrone, Vladimer Darsalia
Type 2 diabetic (T2D) patients often develop early cognitive and sensorimotor impairments. The pathophysiological mechanisms behind these problems are largely unknown. Recent studies demonstrate that dysfunctional γ-Aminobutyric acid (GABAergic) neurons are involved in age-related cognitive decline. We hypothesized that similar, but earlier dysfunction is taking place under T2D in the neocortex and striatum (two brain areas important for cognition and sensorimotor functions). We also hypothesized that the T2D-induced effects are pharmacologically reversible by anti-diabetic drugs targeting the glucagon-like peptide-1 receptor (GLP-1R)...
October 25, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27780891/sin1-promotes-the-proliferation-and-migration-of-breast-cancer-cells-by-akt-activation
#18
Deqiang Wang, Ping Wu, Hui Wang, Lei Zhu, Wei Zhao, Yuqin Lu
Stress-activated protein kinase (SAPK) interacting protein 1 (SIN1) is an essential TORC2 component and a key regulator of Akt pathway which plays an important role in various pathological conditions including cancer. While its functional role in breast cancer has not been well characterized. In our study, SIN1 is associated with the progression and survival of breast cancer patients, as well as human breast cancer cell proliferation and migration. SIN1 mRNA level was significantly upregulated in human breast cancer samples compared with their corresponding para-cancerous histological normal tissues...
October 25, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27780890/novel-protective-effects-of-pulsed-electromagnetic-field-ischemia-reperfusion-injury-rats-and-its-molecular-mechanisms
#19
Fenfen Ma, Wenwen Li, Xinghui Li, Ba Hieu Tran, Rinkiko Suguro, Ruijuan Guan, Cuilan Hou, Huijuan Wang, Aijie Zhang, Yichun Zhu, YiZhun Zhu
Extracorporeal pulsed electromagnetic field (PEMF) has shown the ability to regenerate tissue by promoting cell proliferation. Here, we investigated for the first time whether PEMF treatment could improve the myocardial ischemia/reperfusion (I/R) injury and uncovered its underlying mechanisms. In our study, we demonstrated for the first time that extracorporeal pulsed electromagnetic field(PEMF)has a novel effect on myocardial ischemia/reperfusion(I/R)injury. The number and function of circulating endothelial progenitor cells were increased in PEMF treating rats...
October 25, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27756826/effect-of-polymer-rigidity-on-the-phase-behavior-of-polymer-adsorption-onto-planar-surface
#20
Zhiyong Yang, Aihua Chai, Peicong Zhou, Ping Li, Yongfu Yang
We study the process of a semiflexible polymer chain adsorption onto planar surface by the dynamic Monte Carlo method, based on the three-dimension off-lattice model. Both the strength of attractive monomer-surface interaction εa and bending energy b have pronounced effect on the adsorption and shape of semiflexible polymer chain. The semiflexible polymer can just fully adsorb onto the surface at certain εa which is defined as critical εa The essential features of the semiflexible polymer adsorption onto surface are that (i) the critical εa increases with increase of b ; (ii) the shape of the fully adsorbed semiflexible polymer chain is film-like toroid, and the toroid becomes more and more perfect with increase of b In addition, the size of toroid and the number of turns of toroid can be controlled by the b and εa...
October 18, 2016: Bioscience Reports
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