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Bioscience Reports

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https://www.readbyqxmd.com/read/28733472/effects-of-rna-interference-mediated-gene-silencing-of-vegf-on-the-ultrafiltration-failure-in-a-rat-model-of-peritoneal-dialysis
#1
Zhi-Kui Wang, Zhao-Xia Wang, Zhen-Ying Liu, Yue-Qin Ren, Zhong-Qi Zhou
We investigated the effects of RNA interference-mediated gene silencing of VEGF on ultrafiltration failure (UFF) in rats with peritoneal dialysis (PD). SD male rats were classified into normal, sham operation, and uremic model groups. Uremic rats were sub-categorized into uremia, PD2, VEGF shRNA-2, vector-2, PD2 + Endostar, PD4, VEGF shRNA-4, Vector-4 and PD4 + Endostar groups. Peritoneal Equilibration Test (PET) was conducted to assess ultrafiltration volume (UFV) and mass transfer of glucose (MTG). mRNA and protein expressions of VEGF were detected using qRT-PCR and Western blotting...
July 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28729249/deletion-of-transcription-factor-binding-motifs-using-the-crispr-spcas9-system-in-the-%C3%AE-globin-lcr
#2
Yea Woon Kim, AeRi Kim
Transcription factors play roles in gene transcription through direct binding to their motifs in genome, and inhibiting this binding provides an effective strategy for studying their roles. Here we applied the CRISPR/spCas9 system to mutate the binding motifs of transcription factors. Binding motifs for erythroid specific transcription factors were mutated in the locus control region hypersensitive sites of the human β-globin locus. Guide RNAs targeting binding motifs were cloned into lentiviral CRISPR vector containing the spCas9 gene, and transduced into MEL/ch11 cells carrying a human chromosome 11...
July 20, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28724604/a-simple-toolset-to-identify-endogenous-post-translational-modifications-for-a-target-protein-a-snapshot-of-the-egfr-signaling-pathway
#3
Henrick Horita, Andy Law, Soonjin Hong, Kim Middleton
Identification of a novel post-translational modification (PTM) for a target protein, defining its physiologic role, and studying its potential crosstalk with other PTMs is a challenging process. A set of highly sensitive tools termed Signal-Seeker kits was developed, which enables rapid and simple detection of post-translational modifications on any target protein.  The methodology for these tools utilizes affinity purification of modified proteins from a cell or tissue lysate and immunoblot analysis. These tools utilize a single lysis system that is effective at identifying endogenous, dynamic PTM changes, as well as the potential crosstalk between PTMs...
July 19, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28724603/effects-of-microrna-136-on-melanoma-cell-proliferation-apoptosis-and-epithelial-mesenchymal-transition-by-targeting-pmel-through-the-wnt-signaling-pathway
#4
Jiu-Jiang Wang, Zhi-Feng Li, Xiao-Jing Li, Zhao Han, Ling Zhang, Zhi-Jun Liu
The study aims to evaluate the effects of miR-136 on the proliferation, apoptosis and epithelial mesenchymal transition (EMT) of melanoma cells by targeting PMEL through the Wnt signaling pathway. After establishment of melanoma mouse models, melanoma (model group) and normal tissues (normal group) were collected. Immunohistochemistry was performed to determine PMEL protein concentration. Mouse melanoma cells were assigned into control, blank, negative control (NC), miR-136 mimics, miR-136 inhibitors, siRNA-PMEL, and miR-136 inhibitors + siRNA-PMEL, LiC1 (Wnt signaling pathway activator) and siRNA-PMEL+ LiCl groups...
July 19, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28724602/the-clinical-and-prognostic-value-of-polo-like-kinase-1-in-lung-squamous-cell-carcinoma-patients-immunohistochemical-analysis
#5
Hefei Li, Haibo Wang, Zhenqing Sun, Qiang Guo, Hongyun Shi, Youchao Jia
Polo-like kinase 1 (PLK1) has been suggested to serve as oncogene in most human cancers. The aim of our study is to present more evidence about the clinical and prognostic value of PLK1 in lung squamous cell carcinoma patients. The status of PLK1 was observed in lung adenocarcinoma, lung squamous cell carcinoma and normal lung tissues through analyzing microarray data set (GEO accession number: GSE1213 and GSE 3627). PLK1 mRNA and protein expressions were detected in lung squamous cell carcinoma and normal lung tissues by using qRT-PCR and immunohistochemistry...
July 19, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28710184/diagnostic-value-of-four-dimensional-ct-angiography-in-arterial-erectile-dysfunction-using-320-detector-row-dynamic-volume-ct
#6
Cheng-Cheng Xu, Xin-Zhong Ruan, Yi-Fan Tang, Jiao-Hai Pan, Guo-Yao Wang, Qiu-Li Huang
The study aims to evaluate the diagnostic value of four-dimensional CT angiography (4D-CTA) in the diagnosis of arterial erectile dysfunction (ED) using 320-detector row dynamic volume CT. Arterial ED patients attributed to arterial insufficiency were enrolled. To induce penile erection, an intracavernous injection of corpus cavernosum with a vasoactive drug was administered. Patients were assigned into the erection hardness score (EHS) 1/2 group or EHS 3/4 group. Color duplex Doppler ultrasound (CDDU) was used to analyze blood flow spectrum...
July 14, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28706008/efficacy-and-safety-of-bortezomib-maintenance-in-patients-with-newly-diagnosed-multiple-myeloma-a-meta-analysis
#7
Chun-Yan Sun, Jun-Ying Li, Zhang-Bo Chu, Lu Zhang, Lei Chen, Yu Hu
Multiple myeloma (MM) is a B-cell neoplasm with a high incidence of relapse. Bortezomib has been extensively studied for the maintenance treatment of MM. Here we carried out a meta-analysis to determine the efficacy and safety of maintenance therapy with bortezomib. We searched for clinical trials in PubMed (Medline), Embase (OVID) and the Cochrane Library. Two randomized controlled trials (RCTs) enrolling a total of 1338 patients were included. Bortezomib maintenance statistically significantly improved both PFS (HR 0...
July 13, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28706007/c-reactive-protein-gene-rs1205-polymorphism-is-not-associated-with-the-risk-of-colorectal-cancer
#8
Dilong Fang, Yu Ye
The relationship between C-reactive protein ( CRP ) gene rs1205 polymorphism and the risk of colorectal cancer (CRC) has been investigated previously. However, the results were conflicting. In this study, we assessed whether CRP gene rs1205 polymorphism was associated with the risk of CRC by meta-analysis. We searched in PubMed, Embase, and the CNKI databases. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated. Seven original studies involving 4,181 cases and 10,601 controls analyzed the association between CRP gene rs1205 polymorphism and CRC risk...
July 13, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28694303/single-molecule-fluorescence-microscopy-review-shedding-new-light-on-old-problems
#9
Sviatlana Shashkova, Mark C Leake
Fluorescence microscopy is an invaluable tool in the biosciences, a genuine workhorse technique offering exceptional contrast in conjunction with high specificity of labelling with relatively minimal perturbation to biological samples compared to many competing biophysical techniques. Improvements in detector and dye technologies coupled to advances in image analysis methods have fuelled recent development towards single-molecule fluorescence microscopy, which can utilise light microscopy tools to enable the faithful detection and analysis of single fluorescent molecules used as reporter tags in biological samples...
July 10, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28694302/up-regulation-of-ankdr49-a-poor-prognostic-factor-regulates-cell-proliferation-of-gliomas
#10
Chunyan Hao, Hubin Duan, Hao Li, Mingyang Pei, Yueting Liu, Yimin Fan, Ce Zhang
The Ankyrin repeat domain 49 (ANKRD49) is an evolutionarily conserved protein, which is related to mediate protein-protein interaction. However, the function of ANKRD49 in human glioma remains elusive. Through mining The Cancer Genome Atlas (TCGA) database, we found that the expression of ANKRD49 was increased in glioma tissues and that high expression of ANKRD49 was strongly associated with high disease grade and poor overall survival. To investigate the role of ANKRD49 in malignant glioma, lentivirus expressing shRNA targeting ANKRD49 was constructed in U251 and U87 malignant glioma cells...
July 10, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28684550/frutapin-a-lectin-from-artocarpus-incisa-breadfruit-cloning-expression-and-molecular-insights
#11
Felipe Domingos Sousa, Bruno Bezerra da Silva, Gilvan Pessoa Furtado, Igor de Sa Carneiro, Marina Duarte Pinto Lobo, Yiwei Guan, Jingxu Guo, Alun R Coker, Marcos Roberto Lourenzoni, Maria Izabel Florindo Guedes, James S Owen, David J Abraham, Ana Cristina de Oliveira Monteiro-Moreira, Renato de Azevedo Moreira
Artocarpus incisa (breadfruit) seeds contain three different lectins (Frutalin, Frutapin and Frutackin) with distinct carbohydrate specificities. The most abundant lectin is Frutalin, an α-D-galactose-specific carbohydrate-binding glycoprotein with antitumour properties and potential for tumour biomarker discovery as already reported. Frutapin (FTP) is the second most abundant, but proved difficult to purify with very low yields and contamination with Frutalin frustrating its characterization. Here, we report for the first time high-level production and isolation of biologically-active recombinant FTP in E...
July 6, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28684549/higher-urinary-bpa-concentration-and-excessive-iodine-intake-are-associated-with-ng-and-ptc
#12
Zhenzhen Zhou, Jing Zhang, Fang Jiang, Yan Xie, Xiaochen Zhang, Ling Jiang
In this study, we investigated whether bisphenol A (BPA) levels and excessive iodine intake were associated with papillary thyroid carcinoma (PTC) and nodular goiter (NG). We determined total BPA concentrations (TBC) in paired serum and urine samples, and urinary iodine concentrations (UIC) in urine samples collected from PTC patients, NG patients, and healthy individuals, then compared BPA concentrations and UIC within and between each patient group. The results showed that there was no gender-specific differences in serum TBC and UIC in each group, and no differences across all patient groups...
July 6, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28679650/effects-of-microrna-211-on-proliferation-and-apoptosis-of-lens-epithelial-cells-by-targeting-sirt1-gene-in-diabetic-cataract-mice
#13
Kun Zeng, Qi-Gao Feng, Bao-Tao Lin, Da-Hui Ma, Chun-Min Liu
Our study aimed at exploring the effects of microRNA-211 (miR-211) on the proliferation and apoptosis of lens epithelial cells in diabetic cataract mice by targeting NAD + -dependent histone deacetylase sirtulin 1 (SIRT1). Healthy male mice were assigned to normal and diabetic cataract groups. Blood glucose, lens turbidity and apoptosis were measured. Lens epithelial cells were classified into the normal, blank, negative control (NC), miR-211 mimics, miR-211 inhibitors, siRNA-SIRT1, and miR-211 inhibitors + siRNA-SIRT1 groups...
July 5, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28679649/involvement-of-ca-2-activated-k-channel-3-1-in-hypoxia-induced-pulmonary-arterial-hypertension-and-therapeutic-effects-of-tram-34-in-rats
#14
Shujin Guo, Yongchun Shen, Guangming He, Tao Wang, Dan Xu, Fuqiang Wen
Pulmonary artery hypertension is an incurable disease associated with proliferation of pulmonary artery smooth cells (PASMCs) and vascular remodeling. This study examined whether TRAM-34, a highly selective blocker of calcium-activated potassium channel 3.1 (Kca3.1), can help prevent such hypertension by reducing proliferation of PASMCs. Rats were exposed to hypoxia (10% O2) for 3 weeks and treated daily with TRAM-34 intra-peritoneally from the first day of hypoxia. Animals were sacrificed and examined for vascular hypertrophy, Kca3...
July 5, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28679648/effects-of-microrna-374-on-proliferation-migration-invasion-and-apoptosis-of-human-scc-cells-by-targeting-gadd45a-through-p53-signaling-pathway
#15
Xiao-Jing Li, Zhi-Feng Li, Jiu-Jiang Wang, Zhao Han, Zhao Liu, Bao-Guo Liu
This study investigated the effects of microRNA-374 (miR-374) on human squamous cell carcinoma (SCC) cell proliferation, migration, invasion, and apoptosis through P53 signaling pathway by targeting Gadd45a. Skin samples were collected from patients with skin SCC and normal skin samples. Expression of miR-374, Gadd45a, P53, P73, P16, c-myc, bcl-2, Bax, caspase-3 and caspase-9 were detected using qRT-PCR and western blotting. A431 and SCL-1 cells were divided into blank, negative control (NC), miR-374 mimics, miR374 inhibitors, siRNA-Gadd45a, and miR-374 inhibitors + siRNA-Gadd45a groups...
July 5, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28674107/long-noncoding-rna-dancr-regulates-mir-1305-smad-4-axis-to-promote-chondrogenic-differentiation-of-human-synovium-derived-stem-cells
#16
Lei Zhang, Xiangyi Sun, Shuo Chen, Chao Yang, Ben Shi, Liwu Zhou, Jianning Zhao
MicroRNAs (miRNAs) have been reported to regulate cellular differentiation by modulating multiple signaling pathways. Long noncoding RNA DANCR was previously identified to be critical for the chondrogenesis of human synovium-derived stem cells (SMSC), however, the underlying molecular mechanism requires better understanding. Here, miRNA expression profiling in DANCR overexpressed SMSCs identified significant down-regulation of miR-1305, which serves as a downstream target of DANCR. Notably, miR-1305 overexpression reversed DANCR induced cell proliferation and chondrogenic differentiation of SMSCs, which suggested that miR-1305 antagonized the function of DANCR...
July 3, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28674106/crispr-cas-adaptive-immunity-and-the-three-rs
#17
Tom Killelea, Edward L Bolt
In this summary we focus on fundamental biology of CRISPR-Cas adaptive immunity in bacteria. Emphasis is placed on emerging information about functional interplay between CRISPR associated (Cas) proteins and proteins that remodel DNA during homologous recombination, DNA replication or DNA repair. We highlight how replication forks may act as "trigger-points" for CRISPR adaptation events, and the potential for Cascade interference complexes to act as precise roadblocks to DNA replication by an invader mobile genetic element, without the need for DNA double strand breaks...
July 3, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28667103/expression-and-prognostic-value-of-her-2-neu-in-primary-breast-cancer-with-sentinel-lymph-node-metastasis
#18
Zhen-Jun Tong, Ning-Yao Shi, Zhi-Ji Zhang, Xiao-Dong Yuan, Xiao-Ming Hong
This study explores the correlation of human epidermal growth factor receptor-2 (HER-2) protein expression with sentinel lymph node (SLN) metastasis and prognosis of breast cancer. The breast cancer tissues and adjacent tissues were obtained from patients with primary breast cancer. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the mRNA level of HER-2. Spearman correlation analysis was used to analyze the correlation of HER-2 expression with SLN metastasis. The disease-free survival (DFS) and overall survival (OS) of breast cancer patients were investigated...
June 30, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28667102/adiponectin-improves-coronary-no-reflow-injury-by-protecting-endothelium-in-rats-with-type-2-diabetes-mellitus
#19
Xue Han, Ye Wu, Xin Liu, Lu Ma, Tingting Lv, Qi Sun, Wenli Xu, Suli Zhang, Ke Wang, Wen Wang, Xinliang Ma, Huirong Liu
To determine the effect of adiponectin on the coronary no-reflow (NR) injury in rats with Type 2 Diabetes Mellitus (T2DM), 80 male SD rats were fed with high-sugar and high-fat diet to build T2DM model. Rats received vehicle or Adiponectin (APN) in the last week and then were subjected to myocardial ischemia reperfusion (MI/R) injury. Endothelium dependent vasorelaxation of thoracic aorta was significantly decreased and serum levels of endothelin-1 (ET-1), intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were obviously increased in T2DM rats compared with that in the rats without T2DM...
June 30, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28667101/sigmar1-regulates-endoplasmic-reticulum-stress-induced-c-ebp-homologous-protein-chop-expression-in-cardiomyocytes
#20
Shafiul Alam, Chowdhury S Abdullah, Richa Aishwarya, A Wayne Orr, James Traylor, Sumitra Miriyala, Manikandan Panchatcharam, Christopher B Pattillo, Md Shenuarin Bhuiyan
C/EBP-homologous protein (CHOP) is a ubiquitously expressed stress-inducible transcription factor robustly induced by maladaptive endoplasmic reticulum (ER) stresses in a wide variety of cells. Here we examined a novel function of Sigma 1 receptor (Sigmar1) in regulating CHOP expression under ER-stress in cardiomyocytes. We also defined Sigmar1-dependent activation of the adaptive ER-stress pathway in regulating CHOP expression. We used adenovirus-mediated Sigmar1 overexpression as well as Sigmar1 knockdown by small interfering RNA (siRNA) in neonatal rat ventricular cardiomyocytes; to induce ER-stress cardiomyocytes were treated with tunicamycin...
June 30, 2017: Bioscience Reports
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