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Bioscience Reports

Zhiyong Yang, Aihua Chai, Peicong Zhou, Ping Li, Yongfu Yang
We study the process of a semiflexible polymer chain adsorption onto planar surface by the dynamic Monte Carlo method, based on the three-dimension off-lattice model. Both the strength of attractive monomer-surface interaction εa and bending energy b have pronounced effect on the adsorption and shape of semiflexible polymer chain. The semiflexible polymer can just fully adsorb onto the surface at certain εa which is defined as critical εa The essential features of the semiflexible polymer adsorption onto surface are that (i) the critical εa increases with increase of b ; (ii) the shape of the fully adsorbed semiflexible polymer chain is film-like toroid, and the toroid becomes more and more perfect with increase of b In addition, the size of toroid and the number of turns of toroid can be controlled by the b and εa...
October 18, 2016: Bioscience Reports
Tao Zhang, Hongqiang Chen, Shiyong Qin, Minghai Wang, Xianming Wang, Xin Zhang, Fei Liu, Shuguang Zhang
OBJECTIVE: Whether dietary vitamin A intake could reduce pancreatic cancer risk is still conflicting. We therefore conducted a meta-analysis to summarize the evidence from epidemiological studies. METHODS: We searched the databases of PubMed and Web of Knowledge up to July 2016. Random model was used to combine study-specific relative risks (RR) and 95% confidence interval (95% CI). Publication bias was assessed by Egger regression asymmetry test and Begg's funnel plot...
October 18, 2016: Bioscience Reports
Jiaxin Li, Yingnan Dai, Zhendong Su, Guoqian Wei
To investigate the effects of miR-9 on high glucose (HG)-induced cardiac fibrosis in human cardiac fibroblasts (HCFs), and to establish the mechanism underlying these effects. HCFs were transfected with miR-9 inhibitor or mimic, and then treated with normal or high glucose. Cell viability and proliferation were detected by using the Cell Counting Kit-8 (CCK-8) assay and Brdu-ELISA assay. Cell differentiation and collagen accumulation of HCFs were detected by qRT-PCR and Western blot assays, respectively...
October 18, 2016: Bioscience Reports
Benjamin W Arentson, Erin L Hayes, Weidong Zhu, Harkewal Singh, John J Tanner, Donald F Becker
Proline utilization A (PutA) is a bifunctional flavoenzyme with proline dehydrogenase (PRODH) and Δ(1)-pyrroline-5-carboxylate dehydrogenase domains that catalyzes the two-step oxidation of proline to glutamate. Trifunctional PutAs also have an N-terminal ribbon-helix-helix (RHH) DNA-binding domain and moonlight as autogenous transcriptional repressors of the put regulon. A unique property of trifunctional PutA is the ability to switch functions from DNA-bound repressor to membrane-associated enzyme in response to cellular nutritional needs and proline availability...
October 14, 2016: Bioscience Reports
Mingguang Yan, Weibing Yin, Xiao Fang, Jianjun Guo, Hong Shi
NADH oxidases (NOXs) catalyzing the oxidation of NADH to yield NAD(+) and H2O, H2O2, or both play an important role in protecting organisms from oxidative stress and maintaining the balance of NAD(+)/NADH. A gene encoding NOX was identified from Methanobrevibacter smithii (NOX-ms), the predominant archaeon in the human gut ecosystem. Subsequent analyses showed that it is an FAD-containing protein with a subunit molecular mass of 48 kDa. NOX-ms was purified to homogeneity after expression in Escherichia coli NOX-ms catalyzed the oxidization of NADH and converted O2 to H2O with an optimal pH of 7...
October 13, 2016: Bioscience Reports
Marissa Bacay Roldan, Afsana Islam, Phuong T Y Dinh, Susanna Leung, Michael T McManus
The expression and accumulation of members of the 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) and ACC oxidase (ACO) gene families was examined in white clover roots grown in either Pi (phosphate) sufficient or Pi-deprived defined media. The accumulation of one ACO isoform, TR-ACO1, was positively influenced after only 1 h of exposure to low Pi, and this was maintained over a 7 d time-course. Up-regulation of TR-ACS1, TR-ACS2 and TR-ACS3 transcript abundance was also observed within 1 h of exposure to low Pi in different tissue regions of the roots, followed by a second increase in abundance of TR-ACS2 after 5 - 7 days of exposure...
October 13, 2016: Bioscience Reports
Yulong Tian, Zhongchun Ge, Yuliang Xing, Yan Sun, Jie Ying
Rheumatic heart disease (RHD) is a serious cardiovascular disorder worldwide. Several articles have reported the effect of angiotensin I-converting enzyme gene insertion/deletion (ACE I/D) polymorphism in RHD risk. However, the results still remain inconsistent. The objective of this study was to assess more precise estimations of the relationship between ACE I/D variant and RHD susceptibility. Relevant case-control studies published between January 2000 and 2016 were searched in the electronic databases. The odds ratio (OR) with its 95% confidence interval (CI) was employed to calculate the strength of the effect...
October 10, 2016: Bioscience Reports
Chaohui Dai, Li Sun, Lihuai Yu, Guoqiang Zhu, Shenglong Wu, Wenbin Bao
As a critical adapter protein in Toll-like receptor (TLR)/Interleukin (IL)-1R signaling pathway, Myeloid differentiation protein 88 (MyD88) (plays an important role in immune responses and host defense against pathogens. This study was designed to provide a foundation and an important reagent for the mechanistic study of MyD88  and its role TLR/IL-1R signaling pathways in porcine immunity. Lentivirus-mediated RNAi was used to generate a porcine PK15 cell line with a silenced MyD88  gene and qPCR and Western blotting were used to detect changes in the expression of critical genes in the TLR4 signaling pathway...
October 5, 2016: Bioscience Reports
María M Adeva-Andany, Noemi Pérez-Felpete, Carlos Fernández-Fernández, Cristóbal Donapetry-García, Cristina Pazos-García
Information about normal hepatic glucose metabolism may help to understand pathogenic mechanisms underlying obesity and diabetes mellitus. In addition, liver glucose metabolism is involved in glycosylation reactions and connected with fatty acid metabolism. The liver receives dietary carbohydrates directly from the intestine via the portal vein. Glucokinase phosphorylates glucose to glucose 6-phosphate inside the hepatocyte, ensuring that an adequate flow of glucose enters the cell to be metabolized. Glucose 6-phosphate may proceed to several metabolic pathways...
October 5, 2016: Bioscience Reports
Zohreh Makoolati, Mansoureh Movahedin, Mehdi Forouzandeh-Moghadam
The present study aims to confirm and analyze germ-cell related patterns and specific gene expressions at a very early stage of cell commitment. Following the XY cytogenetic confirmation of the CCE mouse embryonic stem cells (mESCs) line, cells were cultured to form embrioid bodies (EBs). Expression pattern assessment of the Mvh, Stra8, α6 and β1 integrin genes in ESC and 1-3-day-old EB showed that all genes except α6 integrin were expressed in the ESC. The mean calibration of Mvh, Stra8 and α6 integrin expression significantly increased upon EB formation compared to the ESCs...
September 30, 2016: Bioscience Reports
Sunil Bansal, Timothy P Durrett
Euonymus alatus diacylglycerol acetyltransferase ( Ea DAcT) synthesizes the unusually structured 3-acetyl-1,2-diacylgylycerols (acetyl-TAGs) found in the seeds of a few plant species. A member of the membrane-bound O-acyltransferase (MBOAT) family, Ea DAcT transfers the acetyl group from acetyl-CoA to sn -1,2 diacylglycerol (DAG) to produce acetyl-TAGs. In vitro assays demonstrated that the enzyme is also able to utilize butyryl-CoA and hexanoyl-CoA as acyl donors, though with much less efficiency compared to acetyl-CoA...
September 29, 2016: Bioscience Reports
Niannian Fan, Jiantao Wang, Yili Deng, Jie Zhu, Jing Mei, Yao Chen, Heng Yang
Interleukins (ILs) are the most typical inflammatory and immunoregulatory cytokines. Evidences have shown that polymorphisms in ILs are associated with cerebral infarction risk. However, the results remain inconclusive. This study was to evaluate the role of ILs polymorphisms in cerebral infarction susceptibility. Relevant case-control studies published between January 2000 and December 2015 were searched and retrieved from the electronic databases of Web of Science, PubMed, Embase and the Chinese Biomedical Database...
September 27, 2016: Bioscience Reports
Giovanna Castoldi, Cira R T di Gioia, Raffaella Carletti, Francesca Roma, Gianpaolo Zerbini, Andrea Stella
Compound 21 (C21), selective agonist of AT2 receptors, shows antinflammatory effects in hypertension and nephroprotection in diabetes. The aim of this study was to evaluate the effects of C21 in cyclosporine nephropathy, which is characterized mainly by tubulo-interstitial fibrosis. Ten days before and during the experimental periods, low-salt diet was administered to Sprague Dawley rats. Cyclosporine-A (15mg/kg/day, i.p.) and cyclosporine-A plus C21 (0.3 mg/kg /day, i.p) were administered for 1 and 4 weeks...
September 27, 2016: Bioscience Reports
Ute Seeland, Anna Brecht, Ahmad T T Nauman, Sabine Oertelt-Prigione, Mirjam Ruecke, Fabian Knebel, Verena Stangl, Vera Regitz-Zagrosek
OBJECTIVES: This study determines the prevalence of vascular dysfunction and arterial stiffness in a female urban population by measuring the brachial augmentation index (AIx) and aortic pulse wave velocity (PWV). The study tests the hypothesis that the measurement of AIx and PWV is useful in addition to that of traditional cardiovascular risk factors when assessing the risk for left ventricular diastolic dysfunction (LVDD). METHODS: This cross-sectional study recruited 965 women aged 25-75 years from a German major city...
September 21, 2016: Bioscience Reports
Ying Wang, Yijun Sun, Yaochi Wu, Junfeng Zhang
Cucurbitacin E (CuE), a potent member of triterpenoid family isolated from plants, has been confirmed as an antitumor agent by inhibiting proliferation, migration, and metastasis in diverse cancer. However, the effects and mechanisms of CuE on osteosarcoma (OS) have not been well understood. This study aimed to test whether CuE could inhibit growth and invasion of OS cells and reveal its underlying molecular mechanism. After various concentrations of CuE treatment, the anti-proliferative effect of CuE was assessed using the cell counting Kit-8 assay...
September 21, 2016: Bioscience Reports
Georgia Keramida, James Hunter, Adrien Michael Peters
Hepatic steatosis is associated with obesity and insulin resistance. Whether hepatic glucose utilisation rate (glucose phosphorylation rate; MRglu) is increased in steatosis and/or obesity is uncertain. Our aim was to determine the separate relationships of steatosis and obesity with MRglu. Sixty patients referred for routine PET/CT had dynamic PET imaging over the abdomen for 30 min post-injection of F-18-fluorodeoxyglucose (FDG), followed by Patlak-Rutland graphical analysis of the liver using abdominal aorta for arterial input signal...
September 21, 2016: Bioscience Reports
Bohan Cheng, Mengqi Wu, Songsong Xu, Xinyang Zhang, Yuxiang Wang, Ning Wang, Li Leng, Hui Li
The preadipocyte differentiation biological process involves a cascade of transcriptional events that culminates in the expression of peroxisome proliferator-activated receptor (PPAR) γ. The differentiation cocktail (insulin, dexamethasone and isobutylmethylxanthine) can induce preadipocyte differentiation in mammals, but it is insufficient for chicken ( Gallus gallus ) adipogenesis. Oleate can induce chicken preadipocyte differentiation, but these differentiated preadipocytes may not be fully functional. The objective of the current study was to evaluate whether chicken preadipocytes can be induced to mature adipocytes by a novel induction method using differentiation cocktail supplemented with PPARγ agonist(s)...
September 16, 2016: Bioscience Reports
Ru Jiang-Ying, Xu Hai-Dong, Shi Dai, Pan Jun-Bo, Pan Xiao-Jin, Wang Yan-Fen
Ulinastatin(UTI), is widely used to clinically treat LPS-related inflammatory disorders recently. <br />Adherent pathogen-associated molecular patterns (PAMPs),of which LPS is the best-studied<br /> and classical endotoxin produced by Gram-titanium particles in cell culture and animal models of implant loosening.This study was designed to explore the inhibitory effect of UTI on osteoclas-togenesis and inflammatory osteolysis in LPS/PMMA-mediated Raw 264.7 cells and murine osteolysis models ,and investigate the potential mechanism...
September 16, 2016: Bioscience Reports
Glaucia Callera, Tayze Antunes, Jose Correa, Danielle Moorman, Alexey Gutsol, Ying He, Aurelie Nguyen Dinh Cat, Ana M Briones, Augusto C Montezano, Kevin Burns, Rhian M Touyz
High doses of AngII receptor (AT1R) blockers (ARBs) are reno-protective in diabetes. Underlying mechanisms remain unclear. We evaluated whether high/ultra-high doses of candesartan (ARB) upregulate ACE2/Ang II type 2 receptor (AT2R)/Mas receptor (protective axis of the of the renin-angiotensin-system (RAS)) in diabetic mice. Systolic blood pressure (SBP), albuminuria, and expression/activity of RAS components were assessed in diabetic db/db and control db/+mice treated with increasing candesartan doses (intermediate, 1 mg/Kg/d; high, 5 mg/Kg/d; ultra-high, 25 and 75 mg/Kg/d; 4 weeks)...
September 9, 2016: Bioscience Reports
Hanzhi Ding, Hong Quan, Weiguo Yan, Jing Han
Sex determining region Y-box protein 12 (SOX12) is essential for embryonic development and cell fate determination. The role of SOX12 in tumorigenesis of breast cancer is not well-understood. Here, we found that SOX12 mRNA expression was up-regulated in human breast cancer tissues. To clarify the roles of SOX12 in breast cancer, we used lentiviral small hairpin RNAs (shRNAs) to suppress its expression in two breast cancer cells with relatively higher expression of SOX12 (BT474 and MCF-7). Our findings strongly suggested that SOX12 was critical for cell migration and invasion of breast cancer cells...
August 31, 2016: Bioscience Reports
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