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Bioscience Reports

Eugenia Roupakia, Georgios Markopoulos, Evangelos Kolettas
Liu and colleagues investigated the expression of matrix matalloproteinases (MMPs) in human periodontal ligament fibroblasts (hPDLFs) regulated by interleukin-12 (IL-12), a cytokine implicated in inflammatory and immune responses. They showed that IL-12 activates canonical NF-kappaB signalling leading to increased expression of MMP-1 (Interstitial collagenase), MMP-3 (stromelysin 1) and MMP-13 (collagenase 3), and to a smaller reduction in the expression of MMP-2 (gelatinase A) and MMP-9 (gelatinase B) at both mRNA and protein levels, with corresponding changes in the secreted levels of these extracellular matrix-remodelling and immune regulatory metalloproteinases...
March 19, 2018: Bioscience Reports
Yuna Horiuchi, Shao-Jui Lai, Azusa Yamazaki, Ayaka Nakamura, Ryunosuke Ohkawa, Kouji Yano, Takahiro Kameda, Shigeo Okubo, Shitsuko Shimano, Michio Hagihara, Shuji Tohda, Minoru Tozuka
Estimation of the function as well as the amount of high-density lipoprotein (HDL) is required to predict the risk of cardiovascular disease development. Cholesterol efflux capacity (CEC) is the key metric for determining the anti-atherosclerotic function of HDL. However, the assay methods currently used to calculate CEC are not ideal for clinical use as they require the culture of cells. In the present study, we developed a novel CEC assay using immobilized liposome-bound gel beads (ILGs), containing fluorescently labelled cholesterol, as a substitute for cultured cells...
March 15, 2018: Bioscience Reports
Y Deng, X Gan, D Chen, H L Huang, J S Yuan, J M Qiu, S Q Hu, J W Hu, J W Wang
Granulosa cells (GCs) are essential components of follicles and are involved in regulating the process of follicles development. However, comparative studies on GCs isolated from different staged follicles have not been conducted in goose. The aim of the present study was to identify the growth characteristics of goose GCs from pre-hierarchical (6-10 mm) and hierarchical (F4-F2, F1) follicles. Our results showed that the three cohorts of cells had different tolerance to collagenase and had noticeable morphological differences...
March 15, 2018: Bioscience Reports
Nazim Hussain, Wenhua Zhu, Congshan Jiang, Jing Xu, Manman Geng, Xiaoying Wu, Safdar Hussain, Bo Wang, Muhammad Shahid Riaz Rajoka, Yue Li, Juan Tian, Liesu Meng, Shemin Lu
Synoviocytes from rheumatoid arthritis (RA) patients share certain features with tumor cells, such as over proliferation and invasion. Anomalous microRNA (miRNA) expression may participate in the pathogenesis of RA in different ways. The objective of this study was to observe the role of miR-10a-5p targeting TBX5 gene on synoviocyte proliferation and apoptosis in RA. Human synovial sarcoma cell line, SW982 cells stimulating with IL-1β were transfected with miR-10a-5p mimic and siRNA of TBX5. The real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting analysis were used to evaluate the expression level of miR-10a-5p and TBX5 in SW982 cells, respectively...
March 15, 2018: Bioscience Reports
Huisheng Wu, Chaoliang Tang, Lydia Wai Tai, Weifeng Yao, Peipei Guo, Junmou Hong, Xin Yang, Xinyi Li, Zhao Jin, Jianjuan Ke, Yanlin Wang
Background & Aims:  Ischemic stroke has been ranked the second cause of death in patients worldwide.Inflammation which is activated during cerebral ischemia/reperfusion is an important mechanism leading to brain injury. This study aimed to investigate the effect of flurbiprofen axetil on cerebral I/R injury and the role of inflammation in this process. Methods:  Rats were subjected to sham operation or global cerebral I/R with or without flurbiprofen axetil (5 mg/kg or 10 mg/kg). Global cerebral ischemia was achieved by occlusion of bilateral common carotid arteries combined with hypotension for 20 min followed by reperfusion for 72 h...
March 14, 2018: Bioscience Reports
Cheng-Han Yu, Chi-Chi Chou, Der-Yen Lee, Kay-Hooi Khoo, Geen-Dong Chang
Phenyl vinyl sulfone (PVS) and phenyl vinyl sulfonate (PVSN) inactivate protein tyrosine phosphatases (PTPs) by mimicking the phosphotyrosine structure and providing a Michael addition acceptor for the active-site cysteine residue of PTPs, thus forming covalent adducts between PVS (or PVSN) and PTPs. We developed a specific antiserum against PVS. This antiserum can be used in general antibody-based assays such as immunoblotting, immunofluorescence staining, and immunoprecipitation. Target identification through immunoprecipitation and mass spectrometry analysis reveals potential targets of PVS, mostly proteins with reactive cysteine residues or low-pKa cysteine residues that are prone to reversible redox modifications...
March 14, 2018: Bioscience Reports
Anita Ferraretto, Michela Bottani, Paola De Luca, Laura Cornaghi, Francesca Arnaboldi, Margherita Maggioni, Amelia Fiorilli, Elena Donetti
An intestinal 70/30 Caco2/HT-29 co-culture was set up starting from the parental populations of differentiated cells to mimic the human intestinal epithelium. Co-culture was harvested at confluence 0 (T0) and at 3, 6, 10 and 14 days of post-confluence after plating (T3, T6, T10 and T14, respectively) for morphological and functional analysis. Transmission electron microscopy revealed different features from T0 to T14: microvilli and a complete junctional apparatus from T6, mucus granules from T3, as also confirmed by PAS/Alcian Blue staining...
March 14, 2018: Bioscience Reports
Milla-Maria Tauriainen, Ville Männistö, Dorota Kaminska, Maija Vaittinen, Vesa Kärjä, Pirjo Käkelä, Sari Venesmaa, Helena Gylling, Jussi Pihlajamäki
Background & aims : Non-alcoholic fatty liver disease (NAFLD) associates with low levels of serum plant sterols in cross-sectional studies. However, parenterally given plant sterols may lead to liver injury. In addition, it has been suggested that the hepatic sterol transport mechanisms are altered in NAFLD. Therefore, we investigated the association between serum, liver and bile plant sterols and sitostanol with NAFLD. Methods : Out of the 138 individuals (age: 46.3±8.9, BMI: 43.3±6.9 kg/m², 28% men and 72% women), 44 could be histologically categorized to have normal liver, and 94 to have NAFLD...
March 14, 2018: Bioscience Reports
Mao-Chi Weng, Mei-Hui Wang, Jai-Jen Tsai, Yu-Cheng Kuo, Yu-Chang Liu, Fei-Ting Hsu, Hsin-Ell Wang
Regorafenib has been demonstrated in our previous study to trigger apoptosis through suppression of extracellular signal-regulated kinase (ERK)/nuclear factor-κB (NF-κB) activation in hepatocellular carcinoma (HCC) SK-Hep1 cells in vitro However, the effect of regorafenib on NF-κB-modulated tumor progression in HCC in vivo is ambiguous. The aim of the present study is to investigate the effect of regorafenib on NF-κB-modulated tumor progression in HCC bearing mouse model. pGL4.50 luciferase reporter vector transfected SK-Hep1 (SK-Hep1/ luc2 ) and Hep3B 2...
March 13, 2018: Bioscience Reports
Miho Ibi, Sawa Horie, Seiko Kyakumoto, Naoyuki Chosa, Mariko Yoshida, Masaharu Kamo, Masato Ohtsuka, Akira Ishisaki
Many inflammatory cells are known to home to inflamed temporomandibular joint (TMJ) tissues by stimulation with cytokines and chemokines produced by inflammatory lesions in the TMJ. However, how the inflammatory cells affect the progression of inflammation in TMJ synovial tissues after their homing to inflamed TMJ site is still uncertain. Here, we isolated and cultured TMJ synoviocyte-like cells (TMJSCs) from murine TMJ tissues. We demonstrated that interleukin 1b (IL-1β) upregulated expression of monocyte chemoattractant protein 1 (MCP-1) in TMJSCs...
March 13, 2018: Bioscience Reports
Zhenhan Deng, Jianjian Yin, Wei Luo, Ronak Naveenchandra Kotian, Shanshan Gao, Ziqing Yi, Wenfeng Xiao, Wenping Li, Yusheng Li
Chronic non-healing wounds pose a significant challenge to health-care system because of its tremendous utilization of resources and time to heal. It has a well-deserved reputation for reducing the quality of life for those affected and represents a substantial economic burden to the health-care system overall. Earthworms are used as a traditional Chinese medicine, and have been applied pharmacologically and clinically since a long time in China. However, there is paucity in data regarding its wound healing effects...
March 12, 2018: Bioscience Reports
Hui-Lun Lu, Xin-Yan Huang, Yi-Feng Luo, Wei-Ping Tan, Pei-Fen Chen, Yu-Biao Guo
The goal of this study was to investigate the role of M1 macrophages in acute lung injury (ALI). To address this, we used LPS-treated wildtype and CD11b-DTR mice, and examined their M1 macrophage levels, and the extent of their inflammation and pulmonary injuries. In addition, we evaluated pulmonary function by measuring the expressions of SP-A and SP-B in infiltrated M1 macrophages. Finally, we co-cultured the mouse type II-like alveolar epithelial cells (AT-II) and mouse pulmonary microvascular endothelial cells (PMECs) with M1 macrophages in the presence of TNF-α or H2 O2 and assessed them for viability and apoptosis...
March 12, 2018: Bioscience Reports
Liqiang Liu, Lifei Feng, Chengrui Nan, Zongmao Zhao
This study was conducted to investigate the clinical significance of cAMP responsive element binding protein 3 like 1 (CREB3L1) and pleiotrophin (PTN) expression in prognosis of patients with brain gliomas. Human brain tissue samples were collected from normal glial tissues (control), low- and high-grade glioma tissues. CREB3L1 and PTN expression levels in cells were assessed by immunohistochemistry, and population distribution of the CREB3L1- and PTN-presenting patients was examined. The CREB3L1 and PTN mRNA expressions in three types of brain cells were determined by RT-PCR...
March 12, 2018: Bioscience Reports
Chunyan Hao, Hubin Duan, Hao Li, Huan Wang, Yueting Liu, Yimin Fan, Ce Zhang
Mammalian mitochondrial ribosomal proteins are functionally involved in protein synthesis in mitochondrion. Recently numerous studies have illuminated the role of mitochondrion in cancer development. However, the precise function of MRPL42 remains unclear. Here in this study, we identified MRPL42 as a novel oncogene in glioma. By analyzing the Cancer Genome Atlas (TCGA) database, we firstly found that MRPL42 was significantly up-regulated in glioma tissues compared with normal tissues. Functionally, we silenced MRPL42 in glioma cells and revealed that MRPL42 knockdown largely blunted the proliferation of U251 and A172 cells...
March 12, 2018: Bioscience Reports
Hongwei Ma, Tianshi Ma, Miao Chen, Zigui Zou, Zhihong Zhang
Pseudogenes were once regarded as transcriptionally inactive and without specific molecular function. However, current evidence shows that pseudogene-derived long noncoding RNAs (lncRNAs) may be crucial regulators of human cancer development, including gastric cancer (GC). In this study, we report that a pseudogene-derived lncRNA named SFTA1P, which is 693-nucleotide-long, was significantly downregulated in GC tissues compared to that in the adjacent normal tissues. In addition, decreased SFTA1P expression was strongly correlated with advanced TNM stage, larger tumor size, lymphatic metastasis, and poor prognosis of patients with GC...
March 9, 2018: Bioscience Reports
Min Zhang, Min Shi, Yan Zhao
Whether serum copper levels were higher in patients with cervical cancer than that in controls was controversial. Hence, we conducted this study to explore the relationship between serum copper levels and cervical cancer. We searched PubMed, WanFang and China National Knowledge Internet (CNKI) for relevant studies before November 30th, 2017. Standardized mean difference (SMD) and 95% confidence interval (CI) were used to combine results across studies using the random-effect model. A total of 14 publications involving 747 patients with cervical cancer and 1014 controls were eligible through inclusion criteria...
March 8, 2018: Bioscience Reports
Ming-Li Yang, Zhe Huang, Qian Wang, Huan-Huan Chen, Sai-Nan Ma, Rong Wu, Wei-Song Cai
Background: Hepatocellular cancer (HCC) is one of the major causes of cancer related mortality. Genetic polymorphisms may affect the susceptibility and clinical outcome of cancers. We aim to manifest the association of single nucleotide polymorphisms (SNPs) of lncRNA- H19 gene with the risk and prognosis of HCC. Methods: A total of 944 samples composed of 472 HCC patients and 472 matched controls were included in the risk analysis and among them 350 HCC samples were investigated in the prognosis analysis. KASP method was conducted for the SNP genotyping...
March 6, 2018: Bioscience Reports
Guillaume Aw Beaudoin, Timothy S Johnson, Andrew D Hanson
In plants, the hydroxymethylpyrimidine (HMP) and thiazole precursors of thiamin are synthesized and coupled together to form thiamin in plastids. Mutants unable to form HMP can be rescued by exogenous HMP, implying the presence of HMP transporters in the plasma membrane and plastids. Analysis of bacterial genomes revealed a transporter gene that is chromosomally clustered with thiamin biosynthesis and salvage genes. Its closest Arabidopsis homolog, the plastidic nucleobase transporter (PLUTO), is co-expressed with several thiamin biosynthetic enzymes...
March 5, 2018: Bioscience Reports
Qian Wang, Yuan He, Rui Lu, Wen-Ming Wang, Ke-Wu Yang, Hai Ming Fan, Yi Jin, G Michael Blackburn
The New Delhi metallo-β-lactamase (NDM-1) is an important clinical target for antimicrobial research, but there are insufficient clinically useful inhibitors and the details of NDM-1 enzyme catalysis remain unclear. The aim of this work is to provide a thermodynamic profile of NDM-1 catalysed hydrolysis of β-lactams using an isothermal titration calorimetry (ITC) approach and to apply this new method to the identification of new low-molecular-weight dicarboxylic acid inhibitors. The results reveal that hydrolysis of penicillin G and imipenem by NDM-1 share the same thermodynamic features with a significant intrinsic enthalpy change and the release of one proton into solution, while NDM-1 hydrolysis of cefazolin exhibits a different mechanism with a smaller enthalpy change and the release of two protons...
March 5, 2018: Bioscience Reports
Olga N Solovjeva, Marina V Kovina, Maria G Zavialova, Victor G Zgoda, Dmitrii S Shcherbinin, German A Kochetov
Transketolase catalyzes the transfer of a glycolaldehyde residue from ketose (the donor substrate) to aldose (the acceptor substrate). In the absence of aldose, transketolase catalyses the one-substrate reaction that involves only ketose. The mechanism of this reaction was unknown.  Here we show that hydroxypyruvate  serves as a substrate for one-substrate reaction and the reaction product is erythrulose rather than glycol aldehyde, as was shown earlier for xylulose 5-phosphate. The amount of erythrulose released into the medium is equimolar to a double amount of the transformed substrate...
March 2, 2018: Bioscience Reports
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