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Bioscience Reports

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https://www.readbyqxmd.com/read/30413614/association-between-three-genetic-variants-in-kallikrein-3-and-prostate-cancer-risk
#1
Yuan-Yuan Mi, Ke-Wei Ren, Chuang Yue, Jian-Gang Zou, Li Zuo, Li-Feng Zhang, Yu Bai, Atsushi Okada, Takahiro Yasui
BACKGROUND: Epidemiological studies have assessed the association between kallikrein 3 (KLK3) polymorphisms and prostate cancer (PCa) susceptibility. However, published data on this association are somewhat inconclusive. METHODS: Articles investigating the association between three KLK3 (rs1058205, rs2735839, and rs266882) variants and PCa susceptibility were searched from online databases, which included 35,838 patients and 36,369 control participants. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to demonstrate the strength of the association...
November 9, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30413613/decreased-microrna-182-5p-helps-alendronate-promote-osteoblast-proliferation-and-differentiation-in-osteoporosis-via-the-rap1-mapk-pathway
#2
Bao-Long Pan, Zong-Wu Tong, Shu-De Li, Ling Wu, Jun-Long Liao, Yu-Xi Yang, Hu-Huan Li, Yan-Juan Dai, Jun-E Li, Li Pan
Osteoporosis (OP) is a serious health problem that contributes to osteoporotic structural damage and bone fragility. MicroRNAs (miRNAs) can exert important functions over bone endocrinology. Therefore, it is of substantial significance to clarify the expression and function of miRNAs in bone endocrine physiology and pathology to improve the potential therapeutic value for metabolism-related bone diseases. We explored the effect of microRNA-182-5p (miR-182-5p) on osteoblast proliferation and differentiation in OP rats after alendronate (ALN) treatment by targeting ADCY6 through the Rap1/mitogen-activated protein kinase (MAPK) signaling pathway...
November 9, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30413612/enhanced-transfection-of-a-macromolecular-lignin-based-dna-complex-with-low-cellular-toxicity
#3
Yoon Khei Ho, Dan Kai, Geraldine Xue En Tu, G Roshan Deen, Heng Phon Too, Xian Jun Loh
Cationic polymers remain attractive tools for non-viral gene transfer. The effectiveness of these vectors rely on the ability to deliver plasmid DNA (pDNA) into the nucleus of cells. While we have previously demonstrated the potential of Lignin-PGEA-PEGMA as a non-viral gene delivery vector, alterations of cellular phenotype and cytotoxicity were observed post transfection. This study aims to explore transfection conditions for high efficiency and low toxicity of the Lignin-PGEA-PEGMA based gene delivery system...
November 9, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30413611/resetting-the-ligand-binding-site-of-placental-protein-13-galectin-13-recovers-its-ability-to-bind-lactose
#4
Jiyong Su, Linlin Cui, Yunlong Si, Chenyang Song, Yuying Li, Tong Yang, Hao Wang, Kevin H Mayo, Guihua Tai, Yifa Zhou
Placental protein-13 / galectin-13 (Gal-13) is highly expressed in placenta, where its lower expression is related to pre-eclampsia. Recently, the crystal structures of wild type Gal-13 and its variant R53H at high resolution were solved. The crystallographic and biochemical results showed that Gal-13 and R53H could not bind lactose. Here, we used site-directed mutagenesis to re-engineer the ligand binding site of wild type Gal-13, so that it could bind lactose. Of six newly engineered mutants, we were able to solve the crystal structures of three of them (R53HH57R; R53HR55NH57R, and R53HR55NH57R)...
November 9, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30413610/association-of-genetic-polymorphisms-of-sels-and-type-2-diabetes
#5
Long Zhao, Ying-Ying Zheng, You Chen, Yi-Tong Ma, Yi-Ning Yang, Xiao-Mei Li, Xiang Ma, Xiang Xie
BACKGROUND: Selenoprotein S (SleS) gene expression is positively correlated to triglyceride concentrations and is associated with diabetes in animal model. However, the relationship between genetic polymorphisms of SleS and Type 2 diabetes (T2DM) remains unclear. METHODS: In the present study, we genotyped 4 single nucleotide polymorphisms (rs12910524, rs1384565, rs2101171, rs4965814) of SleS gene using TaqMan genotyping method in a case-control study (1947 T2DM patients and 1639 control subjects)...
November 9, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30413609/ndrg3-overexpression-is-associated-with-a-poor-prognosis-in-patients-with-hepatocellular-carcinoma
#6
Ji-Sheng Jing, Hongbo Li, Shun-Cai Wang, Jiu-Ming Ma, La-Qing Yu, Hua Zhou
N-myc downstream-regulated gene 3 (NDRG3), an important member of the NDRG family, is involved in cell proliferation, differentiation, and other biological processes. This study analyzed NDRG3 expression in hepatocellular carcinoma (HCC) and explored the relationship between expression of NDRG3 in HCC patients and their clinicopathological characteristics. We performed quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) analysis and immunohistochemistry (IHC) analyses on HCC tissues to elucidate NDRG3 expression characteristics in HCC patients...
November 9, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30413608/an-efficient-method-for-fitc-labelling-of-proteins-using-tandem-affinity-purification
#7
Lalith K Chaganti, Navneet Venkatakrishnan, Kakoli Bose
Fluorescence-based assays are extremely diverse, sensitive and robust experimental methods for investigating the conformational changes, enzyme kinetics, dynamics and molecular interactions. A prerequisite for most of these experimental approaches is to label the protein of interest with one or more extrinsic fluorophores with desired photophysical properties. Fluorescein isothiocyanate (FITC), due to its high quantum efficiency and conjugate stability is most widely used fluorescence labelling reagent for such experimental approaches...
November 9, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30413607/an-updated-association-between-tnf-%C3%AE-238g-a-polymorphism-and-gastric-cancer-susceptibility-in-east-asians
#8
Hongpeng Zhao, Bo Liu, Lixia Wang, Yanmin Wang, Feng Li, Haihua Yu
Polymorphisms in the tumor necrosis factor alpha (TNF-ᵯC;) gene are emerging as key determinants of gastric diseases. The TNF-α-238G/A single-nucleotide polymorphism is the most extensively studied. However, this association is inconsistent among different populations. We therefore conducted an updated meta-analysis to obtain a more precise estimate of the association of TNF-α-238G/A polymorphism with gastric cancer (GC) risk. A comprehensive search of PubMed, Embase, Chinese (CNKI and WanFang) databases was performed to identify relevant studies through May 5, 2018...
November 9, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30413606/functional-annotation-of-extensively-and-divergently-expressed-mirnas-in-suprachiasmatic-nucleus-of-clock-%C3%A2-19-mutant-mice
#9
Yanli Wang, Ke Lv, Hailong Chen, Mei Zhao, Guohua Ji, Yongliang Zhang, Hongqing Cao, Guanghan Kan, Yinghui Li, Lina Qu
Circadian locomotor output cycles kaput protein (CLOCK) is a core transcription factor of complex integrated feedback loops in mammalian circadian clock. More genes have been reported to be regulated by CLOCK, however little is known about the role of CLOCK-mediated miRNAs. To dissect this, we used microarray analysis to measure miRNAs expression in suprachiasmatic nuclei (SCN) of wild type (WT) and Clock Δ19 mutant mice at two different time points. We found that miRNAs regulation in two time points was extensive (nearly 75% of the miRNAs expressed at each time point), and very little overlap, with only 6 miRNAs in common...
November 9, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30413605/upregulated-and-interrelated-expressions-of-gins-subunits-predict-poor-prognosis-in-hepatocellular-carcinoma
#10
Yi-Fan Lian, Shan-Shan Li, Yan-Lin Huang, Huan Wei, Dong-Mei Chen, Jia-Liang Wang, Yue-Hua Huang
The GINS complex is one of the core components of the eukaryotic replicative helicase CMG (Cdc45-MCM helicase-GINS) complex that serves as the replicative helicase unwinding duplex DNA ahead of moving replication fork during chromosome duplication. Many studies have highlighted the important functions among GINS subunits in various cancers. Nevertheless, the functions and prognostic roles of distinct GINS subunits in hepatocellular carcinoma (HCC) were largely unexplored. In this study, we reported the prognostic values of GINS subunits in HCC patients through analysis of several databases, including Oncomine, TCGA and Kaplan-Meier Plotter...
November 9, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30413604/association-between-scn1a-polymorphism-rs3812718-and-valproic-acid-resistance-in-epilepsy-children-a-case-control-study-and-meta-analysis
#11
Zhi Jian Wang, Jie Chen, Hai Liang Chen, Lin Yan Zhang, Duo Xu, Wen Ting Jiang
Resistance to valproic acid (VPA), a first-line antiepileptic drug (AED), is occurring at an alarming rate, particularly in children. Signal nucleotide polymorphisms are considered crucial in this process. Therefore, we investigated whether the SCN1A polymorphism rs3812718 could be associated with VPA resistance. A total of 231 children with epilepsy who were solely administered VPA were enrolled. DNA was extracted from the peripheral blood samples and was genotyped by the Mass Array method. Furthermore, a meta-analysis was conducted between the drug responsive and resistant patients who were exposed to voltage-gated sodium channels...
November 9, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30401732/commentary-on-ivancic-et-al-enzyme-kinetics-from-circular-dichroism-of-insulin-reveals-mechanistic-insights-into-the-regulation-of-insulin-degrading-enzyme
#12
Giuseppe Grasso
Despite the enormous number of therapeutic advances in medicine, nowadays many diseases are still incurable, mainly due to the lack of knowledge of the pathological biochemical pathways triggering those diseases. For this reason, it is compulsory for the scientific community to investigate and unveil the biomolecular mechanisms responsible for the development of those diseases, such as Alzheimer's disease (AD) and diabetes, which are widespread all over the world. In this scenario, it is of paramount importance to develop new analytical techniques and experimental procedures which are capable to make the above mentioned investigations feasible...
November 6, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30401731/combination-of-transcriptome-sequencing-and-itraq-proteome-reveal-the-molecular-mechanisms-determining-petal-shape-in-herbaceous-peony-paeonia-lactiflora-pall
#13
Yanqing Wu, Yuhan Tang, Yu Jiang, Daqiu Zhao, Jiali Shang, Jun Tao
The molecular mechanisms controlling petal shape in a herbaceous peony, Paeonia lactiflora Pall., a popular high-grade cut flower worldwide, remain unclear. Here, we selected inner and outer petals from P. lactiflora 'ZiFengyu' with an anemone type as the study object. Using transcriptome sequencing and isobaric tags for relative and absolute quantitation (iTRAQ) proteome, 979 differentially expressed genes and 266 differentially expressed proteins were detected within the inner and outer petals. Of these, the present study identified a key gene APETALA2 that regulates flower shape development...
November 6, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30401730/dietary-protein-intake-and-risk-of-ovarian-cancer-evidence-from-a-meta-analysis-of-observational-studies
#14
Yanyang Pang, Wu Wang
The association between dietary protein intake and ovarian cancer had been inconsistent in the previous epidemiological studies. The aim of this study was to identify and synthesize all citations evaluating the relationship on ovarian cancer with protein intake. The search included PubMed, Embase, and Web of Science from inception to June 2018. Two authors independently selected studies, extracted data and assessed risk of bias. RR and 95%CI were calculated for relationship between the dietary protein intake and ovarian cancer risk using a random-effects model...
November 6, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30401729/casticin-inhibits-breast-cancer-cell-migration-and-invasion-by-downregulation-of-pi3k-akt-signaling-pathway
#15
Li Fan, Yi Zhang, Qiuhong Zhou, Ying Liu, Baolan Gong, Jieyu Lü, Hui Zhu, Guijuan Zhu, Yingping Xu, Guangrong Huang
Casticin is one of the major active components isolated from Fructus viticis Increasing studies have revealed that casticin has potential anti-cancer activity in various cancer cells, but its effects on breast cancer cell migration and invasion are not well known. Therefore, the ability of cell migration and invasion in the breast cancer MDA-MB-231 and 4T1 cells treated by casticin was investigated. The results indicated that casticin significantly inhibited cell migration and invasion in the cells exposed to 0...
November 6, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30393234/gene-expression-profiles-analysis-identifies-a-novel-two-gene-signature-to-predict-overall-survival-in-diffuse-large-b-cell-lymphoma
#16
Chengtao Sun, Xianfeng Cheng, Chaoyu Wang, Xi Wang, Bing Xia, Yizhuo Zhang
Diffuse large B cell lymphoma (DLBCL) is the most common hematologic malignancy, however, specific tumor associated genes and signaling pathways have yet to be deciphered. Differentially expressed genes (DEGs) were computed based on gene expression profiles from GSE32018, GSE56315 and TCGA DLBC. Overlapping DEGs were then evaluated for gene ontology (GO), pathways enrichment, DNA methylation, protein-protein interaction (PPI) network analysis as well as survival analysis. 74 upregulated and 79 downregulated DEGs were identified...
November 4, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30393233/rapamycin-inhibits-proliferation-and-induces-autophagy-in-human-neuroblastoma-cells
#17
Xiaokun Lin, Lei Han, Jialei Weng, Kelai Wang, Tongke Chen
OBJECTIVE:  To investigate the effect of Rapamycin on proliferation and autophagy in human neuroblastoma (NB) cell lines and to elucidate the possible mechanism. METHODS:  NB cells were treated with different concentrations of Rapamycin. Cell counting kit-8 (CCK-8) was used to measure proliferation, and flow cytometry (FCM) was used to analyze the cell cycle. Electron microscopy was used to observe cell morphological changes. Western blotting (WB) was performed to detect the expression of Beclin-1, LC3-I/II, P62, mammalian target of Rapamycin (mTOR) and p-mTOR...
November 4, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30393232/metabolomics-analysis-of-gut-barrier-dysfunction-in-a-trauma-hemorrhagic-shock-rat-model
#18
Zhongqi Li, Jian Li, Shouwei Zhang, Gang Chen, Shaohua Chi, Xugang Li, Fei Guo, Jianbo Zhu, Baoxi Sun
Intestinal barrier dysfunction has been implicated in the development of multiorgan dysfunction syndrome (MODS) caused by the trauma-hemorrhagic shock (THS). However, the mechanisms underlying THS-induced gut barrier injury are still poorly understood. In this study, we used the metabolomics analysis to test the hypothesis that altered metabolites might be related to the development of THS-induced barrier dysfunction in the large intestine. Under the induction of THS, gut barrier failure was characterized by injury of permeability and mucus layer, which were companied by the decreased expression of zonula occludens (ZO)-1 in the colon and increased levels of inflammatory factors including tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-6 and IL-1β in the serum...
November 4, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30393231/maggot-protein-ameliorates-dextran-sulfate-sodium-induced-ulcerative-colitis-in-mice
#19
Rong Wang, Lei Wang, Yongzheng Luo, Daojuan Wang, Ronghui Du, Jiancheng Du, Yong Wang
Ulcerative colitis (UC) is a common chronic remitting disease but without satisfactory treatment. Maggots are known as a traditional Chinese medicine named as "wu gu chong". The aim of this study was to investigate the therapeutic effect of the maggot protein on dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice. In this study, female C57BL/6 mice were given sterile water containing 3% DSS to establish the model of UC. Mice were randomly divided into five groups: control group (sterile water), model group (DSS), treatment group (DSS + maggot protein), mesalazine group (DSS + mesalazine), and maggot protein group (sterile water + maggot protein)...
November 4, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30389712/rhotekin-2-silencing-inhibits-proliferation-and-induces-apoptosis-of-human-osteosarcoma-cells
#20
Xiong Wang, Lei Zhang, Wenji Wang, Yuchen Wang, Ye Chen, Ruimin Xie, Xiang Li, Yongping Wang
Synopsis Human osteosarcoma is the most frequent primary malignant of bone, and often occurs in adolescents. However, molecular mechanism of this disease remains unclear. In this study, we found that the level of Rhotekin 2 (RTKN2) was up-regulated in osteosarcoma tissues and cell lines. In addition, Silencing of RTKN2 of human osteosarcoma cell lines U2OS, inhibited proliferation and induced G1 phase cell cycle arrest via reducing the level of the CDK2. Furthermore, RTKN2 knockdown in the U2OS cells induced apoptosis by increasing the level of Bax and decreasing the level of Bcl2...
November 2, 2018: Bioscience Reports
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