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Bioscience Reports

Li Zhang, Huaijun Liu, Xiaodan Mu, Jianling Cui, Zhigang Peng
Aberrant expression of Fos-related antigen-1 (Fra1) is commonly elevated in various malignant cancers and is strongly implicated in invasion and metastasis. However, the molecular mechanisms underlying its dysregulation in human glioma remain poorly understood. In the present study we demonstrate that upregulation of Fra1 plays a crucial role in the glioma aggressiveness and epithelial-mesenchymal transition activated by Wnt/β-catenin signal pathway. In glioma cells, activation of Wnt/β-catenin signaling by Wnt3a administration obviously induced epithelial-mesenchymal transition (EMT) and directly activated the transcription of Fra1...
February 23, 2017: Bioscience Reports
Yuchen Zhang, Junjun Cheng, Fang Chen, Changyan Wu, Junmeng Zhang, Xuejun Ren, Yu Pan, Bin Nie, Quan Li, Yu Li
Background : Microparticles (MPs) and microRNAs (miRNAs) have been shown to play important roles in coronary artery disease (CAD) by monitoring endothelial dysfunction. This study aims to investigate the diagnostic value of endothelial microparticles (EMPs) and miRNAs (miR-92a or miR-23a) as biomarkers in distinguishing patients with acute myocardial infarction (AMI) from those with CAD. Methods : Plasma samples from 37 patients with AMI, 42 patients with stable coronary artery disease (SCAD) and 35 healthy adults were collected for investigation in this study...
February 17, 2017: Bioscience Reports
Youngtak Cho, Narae Shin, Daesan Kim, Jae Yeol Park, Seunghun Hong
This paper provides a concise review on the recent development of nanoscale hybrid systems based on carbon nanotubes (CNTs) for biological sensing and control. CNT-based hybrid systems have been intensively studied for versatile applications of biological interfaces such as sensing, cell therapy, and tissue regeneration. Recent advances in nanobiotechnology not only enable the fabrication of highly-sensitive biosensors at nanoscale but also allow the applications in the controls of cell growth and differentiation...
February 10, 2017: Bioscience Reports
Dawei Wang, Guoliang Lu, Yuan Shao, Da Xu
Objective: MicroRNAs (miRNAs) are a class of non-coding RNAs that exert critical roles in various biological processes. The target of the present study was to identify the functional roles of microRNA-802 (miR-802) in regulating epithelial-mesenchymal transition (EMT) in prostate cancer (PCa). Methods: miR-802 expression was investigated in 73 pairs of PCa samples and PCa cell lines (PC3 and DU145 cells) by qRT-PCR. Cell proliferation was detected using MTT assay, and cell apoptosis was evaluated using flow cytometry...
February 10, 2017: Bioscience Reports
Jia Zheng, Jing Cheng, Qian Zhang, Xinhua Xiao
Recent epidemiological and clinical studies have shown that type 2 diabetic patients can develop diabetic vascular complications even after intensive glycemic control. It has been suggested that this phenomenon could be explained by the hypothesis of "metabolic memory". The underlying mechanisms between these enduring effects and the prior hyperglycemic state are still not well understood. Preliminary studies demonstrate that hyperglycemia can regulate gene expression by epigenetic modifications, such as DNA methylation, which can persistently exist even after glucose normalization...
February 9, 2017: Bioscience Reports
Zhi-da Wang, Yu-Fei Xia, Yue Zhao, Li-Ming Chen
This meta-analysis was performed to evaluate the effect of cognitive behavioral therapy (CBT) in improving the depression symptoms of patients with diabetes. Literature search was conducted in PubMed and Embase up to Oct 2016 without initial date. The pooled SMD (standard mean difference) and its 95% confidence interval (CI) were calculated by Revman 5.3. Subgroup analyses were performed by type of diabetes and evaluation criteria of depression. A total of 5 randomized control trials involving 834 patients with diabetes mellitus (including 417 patients in CBT group and 417 patients in control group) were included in this meta-analysis...
February 9, 2017: Bioscience Reports
Hui Zhang, Changjun Liu, Hui Jiang, Lu Zhou, Wenying Li, Ling-Yun Zhu, Lei Wu, Er Meng, Dong-Yi Zhang
Molecular cloning methods based on primer and overlap extension PCR are widely used due to their simplicity, reliability,low cost and high efficiency.  In this article, an efficient mega primer (MP)-mediated cloning strategy for chimeragenesis and long DNA fragment insertion is presented.  MP cloning is a seamless, restriction/ligation-independent method that requires only three steps: i) the first PCR for mega primer generation; ii) the second PCR for exponential amplification mediated by the mega primers; and iii) DpnI digestion and transformation...
February 9, 2017: Bioscience Reports
Yan Wang, Gang Li, Man-Zhen Zuo, Hai-Rong Li, Jun-Hua Fang, Dan-Dan Quan, Lu Huang, Ping-Ping Peng
Objective: This study aims to explore the relationship between the Y chromosome polymorphisms (1qh+, inv(9), 9qh+, 16qh+, group D/G, Yqh- and Yqh+) and the risk of unexplained recurrent miscarriage (URM).Methods: A total of 507 couples with URM were recruited as case group and 465 healthy couples as control group. The Y chromosome polymorphisms of the male individuals were analyzed with the G-banding technique, and the results of the chromosome G-banding analysis were determined using the International Naming Standards of Human Genetics (ISCN)...
February 9, 2017: Bioscience Reports
Xianghui Sun, Lei Zhang
MicroRNAs are non-coding RNAs that have functions to regulate gene expression and play essential roles in a variety of biological processes of cancers. In this study, we report miR-143 acts as a tumor suppressor in human oral squamous cell carcinoma (OSCC). The expressions of miR-143 are downregulated in both oral squamous cell carcinoma cell lines and patient samples compared with normal adjacent tissues. We found overexpression of miR-143 in oral cancer cell lines suppresses cell migration, cellular glucose metabolism and proliferation...
February 7, 2017: Bioscience Reports
Osman Ugur Sezerman, Gunseli Bayram Akcapinar
Metal ions play pivotal roles for protein structure, function and stability. The functional and structural diversity of proteins in nature expanded with the incorporation of metal ions or clusters in proteins. Approximately one-third of these proteins in the databases contain metal ions.  Many biological and chemical processes in nature involve metal ion binding proteins, aka metalloproteins. Many cellular reactions that underpin life require metalloproteins. Most of the remarkable, complex chemical transformations are catalyzed by metalloenzymes...
February 6, 2017: Bioscience Reports
Hani Harb, James Irvine, Manori Amarasekera, Charles S Hii, Dörthe A Kesper, Yuefang Ma, Nina D'Vaz, Harald Renz, Daniel P Potaczek, Susan L Prescott, Antonio Ferrante
While immunodeficiency of immaturity of the neonate has been considered important as the basis for unusual susceptibility to infection, it has also been recognized that the ability to progress from an immature Th2 cytokine predominance to a Th1 profile has relevance in determining whether children will develop allergy, providing an opportunity for epigenetic regulation through environmental pressures. However, this notion remains relatively unexplored. Here we present evidence that there are two major control points to explain the immunodeficiency in cord blood (CB) T-cells, a deficiency in interleukin-12 (IL-12) producing and IL-10 overproducing accessory cells, leading to a decreased interferon-γ (IFNγ) synthesis, and the other an intrinsic defect in T-cell protein kinase C zeta (PKCζ) expression...
February 3, 2017: Bioscience Reports
GuoHua Ma, Bing Pan, Yue Chen, CaiXia Guo, MingMing Zhao, LeMin Zheng, BuXing Chen
Several studies have reported a strong association between high plasma level of Trimethylamine N-oxide (TMAO) and atherosclerosis development. However, the exact mechanism underlying this correlation is unknown. In this study, we try to explore the impact of TMAO on endothelial dysfunction. After TMAO treatment, Human umbilical vein endothelial cells (HUVECs) showed significant impairment in cellular proliferation and HUVECs-ECM adhesion compared to control. Likewise, TMAO markedly suppressed HUVECs migration in transwell migration assay and wound healing assay...
February 2, 2017: Bioscience Reports
Ling-Xu Wang, Na Wang, Qing-Li Xu, Wei Yan, Li Dong, Bao-Lin Li
The study aims to investigate the effect of vitamin D (VD) combined with pioglitazone hydrochloride (PIO) on bone mineral density (BMD) and bone metabolism in patients with type 2 diabetic nephropathy (T2DN). T2DN patients were selected and assigned into mild, moderate and severe groups. In each group, three therapy regimens (VD, PIO, and VD plus PIO) were administrated. X-ray absorptiometry was used to measure BMD. Intact parathyroid hormone (iPTH) and 25-OH-VD3 were measured by chemiluminescence meter. ELISA was applied to detect levels of osteoprotegerin (OPG), bone gla protein (BGP), C-terminal telopeptides of type I collagen (β-CTX), procollagen type I N propeptide (PINP), pyridinoline (Pyr), and deoxypyridinoline (D-Pyr)...
February 2, 2017: Bioscience Reports
Run-Tian Liu, Jing-Lin Cao, Chang-Qing Yan, Yang Wang, Cong-Jing An, Hai-Tao Lv
This study explored the effect of LncRNA-HOST2 on cell proliferation, migration, invasion and apoptosis of human hepatocellular carcinoma (HCC) cell line SMMC-7721. HCC tissues and adjacent normal tissues from 162 HCC patients were collected. The HCC cell lines were assigned into the control group (regular culture), negative control group (NC, transfected with siRNA) and experimental group (transfected with Lnc-HOST2 siRNA). qRT-PCR was used to detect the expression of LncRNA-HOST2. Cell proliferation was detected by CCK-8 and colony-forming assays, cell apoptosis by flow cytometry and cell migration by scratch test...
January 31, 2017: Bioscience Reports
Hao Feng, Li Wang, Min Zhang, Zhiwei Zhang, Wei Guo, Xietong Wang
Preeclampsia is a condition unique to pregnancy, and abnormal expression of matrix metalloproteinases (MMPs) has been implicated in its pathogenesis. We aimed to evaluate the reliability of plasma levels of MMP-2, MMP-9 and their relative ratio in predicting preeclampsia. A total of 318 women with suspected preeclampsia were recruited for the study, who were subsequently either cleared or diagnosed of preeclampsia and grouped accordingly. Their baseline characteristics were compared. Blood samples were also collected from all participants, to determine the plasma levels of MMP-2 and MMP-9...
January 31, 2017: Bioscience Reports
María D Oliver, Teresa Fernández-Acero, Sandra Luna, Isabel Rodríguez-Escudero, María Molina, Rafael Pulido, Victor J Cid
In higher eukaryotes, cell proliferation is regulated by class I phosphatidylinositol 3-kinase (PI3K), which transduces stimuli received from neighboring receptors by local generation of PtdIns(3,4,5)P3 in cellular membranes. PI3K is a heterodimeric protein consisting of a regulatory and a catalytic subunit (p85 and p110, respectively). Heterologous expression of p110a in S. cerevisiae leads to toxicity by conversion of essential PtdIns(4,5)P2 into futile PtdIns(3,4,5)P3, providing a humanized yeast model for functional studies on this pathway...
January 31, 2017: Bioscience Reports
Fei Wang, Zhi-Rong Zou, Dong Yuan, Yi Gong, Li Zhang, Xun Chen, Tao Sun, Hua-Lin Yu
The present study is designed to explore the correlation between serum S100β level and cognitive dysfunction in patients with cerebral small vessel disease (SVD). A total of 172 SVD patients were participated into the study, and they were assigned into patients with no cognitive impairment (NCI group) and those with vascular cognitive impairment no dementia (VCIND group). Besides, 105 people were recruited as the normal control group. Serum S100β protein level was detected by ELISA. Receiver operating characteristic (ROC) curve was employed the predictive value of serum S100β in diagnosing SVD with cognitive dysfunction...
January 31, 2017: Bioscience Reports
Yong-Eun Kim, Sun-Kyung Choi, Jong Ok Kim, Kee Kwang Kim
RBFOX3, a nuclear RNA-binding protein, is well known as a regulator of alternative pre-mRNA splicing during neuronal development. However, other functions of RBFOX3 are poorly understood. Here, we investigated the function of RBFOX3 in the cytoplasm with respect to regulation of Claudin-1 expression. In human lung tissue, Claudin-1 is higher in RBFOX3-positive cells than in RBFOX3-negative cells. Immunostaining and mRNA quantification revealed that protein levels, but not mRNA levels, of Claudin-1 are increased by RBFOX3...
January 26, 2017: Bioscience Reports
Suprama Datta, Uday S Annapore, David J Timson
Aldehyde dehydrogenases play crucial roles in the detoxification of exogenous and endogenous aldehydes by catalyzing their oxidation to carboxylic acid counterparts. This study reports characterization of two such isoenzymes from the yeast Saccharomyces cerevisiae var. boulardii (NCYC 3264), one mitochondrial (Ald4p) and one cytosolic (Ald6p). Both Ald4p and Ald6p were oligomeric in solution and demonstrated positive kinetic cooperativity towards aldehyde substrates. Wild-type Ald6p showed activity only with aliphatic aldehydes...
January 26, 2017: Bioscience Reports
En-Hui Li, Qin-Zhu Huang, Gao-Chun Li, Zhen-Yang Xiang, Xin Zhang
This study explored the effect of microRNA-200b (miR-200b) on the development of diabetic retinopathy (DR) by targeting VEGFA gene. The study populations consisted of 255 DR patients (case group) and 253 healthy people (control group), while the expressions of miR-200b and VEGFA mRNA were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Bioinformatics software and dual-luciferase reporter assay were used to confirm VEGFA as a target gene of miR-200b. Also, a total of 70 Wistar male rats were selected and randomly assigned into blank, normal control (NC), miR-200b mimics, miR-200b inhibitors, miR-200b inhibitors + siVEGFA and siVEGFA groups (n = 10/group) respectively...
January 25, 2017: Bioscience Reports
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