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Bioscience Reports

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https://www.readbyqxmd.com/read/28442601/crystal-structure-of-thermostable-alkylsulfatase-sdsap-from-pseudomonas-sp-s9
#1
Lifang Sun, Pu Chen, Yintao Su, Zhixiong Cai, Lingwei Ruan, Xun Xu, Yunkun Wu
A novel alkylsulfatase from bacterium Pseudomonas sp. S9 (SdsAP) was identified as a thermostable alkylsufatases (type III) which could hydrolyze the primary alkyl sulfate such as sodium dodecyl sulfate (SDS). Thus, it has a potential application of SDS bio-degradation. The crystal structure of SdsAP has been solved to a resolution of 1.76 Å and reveals that SdsAP contains the characteristic metallo-β-lactamase like fold domain, dimerization domain and C-terminal sterol carrier protein type 2 like (SCP-2-like) fold domain...
April 25, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28442600/effect-of-rivaroxaban-on-preventing-deep-vein-thrombosis-in-aged-diabetics-with-femoral-neck-fractures-after-hip-replacement
#2
Yi-Min Zhang, Xin Jiang, Yan-Shan Sun
This study estimates the effect of rivaroxaban on preventing deep-vein thrombosis (DVT) in aged diabetics with femoral neck fractures after hip replacement. Our study was composed of 236 aged diabetics with femoral neck fractures, which were divided into the rivaroxaban and control groups. Reaction time (R time), clot formation time (K time), alpha angle (α), maximum amplitude (MA), clot elasticity (G) and coagulation index (CI), prothrombin time (PT) and activated partial thromboplastin time (APTT) were measured...
April 25, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28442599/overcoming-cisplatin-resistance-in-osteosarcoma-through-the-mir-199a-modulated-inhibition-of-hif-1%C3%AE
#3
Ajimu Keremu, Abudureyimu Aini, Yusufuaji Maimaitirexiati, Zhilin Liang, Pazila Aila, Paizila Xierela, Aikebaier Tusun, Hanikezi Moming, Aihemaitijiang Yusufu
Dysregulation of microRNAs has been shown to contribute to multiple tumorigenic processes, as well as to correlate with tumor progression and prognosis. MiR-199a has been shown to be dysregulated in multiple tumor types. However, the association between miR-199a and the chemoresistance features of osteosarcoma is not well understood, and the target genes for miR-199a and the regulatory mechanisms are also unknown. In this study, we demonstrated that miR-199a is expressed at low levels in osteosarcoma cells and patient samples...
April 25, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28432272/establishment-of-an-in-vitro-culture-model-of-theca-cells-from-hierarchical-follicles-in-ducks
#4
Xiang Gan, da Chen, Yan Deng, Junsong Yuan, Bo Kang, Jiamin Qiu, Wenqiang Sun, Chunchun Han, Jiwei Hu, Liang Li, Jiwen Wang
Theca cells, including theca interna cells and theca externa cells, are vital components of ovarian follicles. The aim of this study is to identify a reliable method for the in vitro culture of theca cells from duck ovarian hierarchical (F4-F2) follicles. We improved the method for cell separation by using trypsin to further remove granular cells, and we increased the concentration of fetal bovine serum used in in vitro culture to improve cytoactivity. Cell antibody immunofluorescence showed that all inoculated cells could be stained by the CYP17A1/19A1 antibody but not by the FSHR antibody, which could stain granulosa cells...
April 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28432271/knockdown-of-prdx2-sensitizes-colon-cancer-cells-to-5-fu-by-suppressing-the-pi3k-akt-signaling-pathway
#5
Jun Xu, Shouru Zhang, Rong Wang, Xingye Wu, Li Zeng, Zhongxue Fu
Although, 5-Fluorouracil (5-FU) remains widely used in adjuvant therapy in patients with colon cancer, resistance to 5-FU-based chemotherapy is an important reason for treatment failure. Recent studies have reported that an enhanced reactive oxygen species (ROS) scavenging system shows drug resistance to 5-FU. Peroxiredoxin-2 (PRDX2), is an important member of the ROS scavenging system, and may be a potential target that promotes chemosensitivity to 5-FU in colon cancer. Here, we depleted PRDX2 by PRDX2-shRNA-LV transduction in two colon cancer cell lines and found that in vitro PRDX2 knockdown facilitates cell death and apoptosis in 5-FU-treated colon cancer cells...
April 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28428431/the-association-between-dietary-zinc-intake-and-risk-of-pancreatic-cancer-a-meta-analysis
#6
Li Li, Xuesong Gai
Previous reports have suggested a potential association on dietary zinc intake with the risk of pancreatic cancer. Since the associations between different studies were controversial, we therefore conducted a meta-analysis to re-assess the relationship between dietary zinc intake and pancreatic cancer risk. A comprehensive search from the databases of PubMed, Embase, Web of Science and Medline was performed until 31th of January in 2017. Relative Risk (RR) with 95% Confidence Intervals (CI) derived by using random effect model was used...
April 20, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28428430/collagen-beta-1-o-galactosyltransferase-1-glt25d1-is-required-for-the-secretion-of-high-molecular-weight-adiponectin-and-affects-lipid-accumulation
#7
Julie A Webster, Zhe Yang, Yu-Hee Kim, Dorothy Loo, Rasha M Mosa, Hongzhuo Li, Chen Chen
Secretion of high molecular weight (HMW) adiponectin is dependent on post-translational modification (PTM) of conserved lysines in the collagenous domain. This work aims to characterize the enzymes responsible for the PTM of conserved lysines which leads to HMW adiponectin secretion, and to define its significance in relation to obesity.  Collagen beta (1- O ) galactosyltransferase 1 (GLT25D1) was knocked down in HEK cells modified for the stable expression of adiponectin (Adipo-HEK cells) as well as in Simpson Golabi-Behmel-Syndrome (SGBS) adipocytes...
April 20, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28424372/effects-of-drought-stress-on-global-gene-expression-profile-in-leaf-and-root-samples-of-dongxiang-wild-rice-oryza-rufipogon
#8
Fantao Zhang, Yi Zhou, Meng Zhang, Xiangdong Luo, Jiankun Xie
Drought is a serious constraint to rice production throughout the world, and although Dongxiang wild rice ( Oryza rufipogon , DXWR) possesses a high degree of drought resistance, the underlying mechanisms of this trait remains unclear. In the present study, cDNA libraries were constructed from the leaf and root tissues of drought-stressed and untreated DXWR seedlings, and transcriptome sequencing was performed with the goal of elucidating the molecular mechanisms involved in drought stress response. The results indicated that 11,231 transcripts were differentially expressed in the leaves (4,040 up-regulated and 7,191 down-regulated) and 7,025 transcripts were differentially expressed in the roots (3,097 up-regulated and 3,928 down-regulated)...
April 19, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28424371/comprehensive-assessmentof-cavernosography-with-320-row-dynamic-volume-ct-versus-conventional-cavernosography-in-erectile-dysfunction-patients-caused-by-venous-leakage
#9
Cheng-Cheng Xu, Yu-Ning Pan, Yi-Fan Tang, Jie Zhang, Guo-Yao Wang, Qiu-Li Huang
This study aims to investigate and compare the diagnostic and prognostic value of cavernosography with 320-row dynamic volume computed tomography (DVCT) versus conventional cavernosography in men with erectile dysfunction (ED) caused by venous leakage. A total of 174 patients diagnosed with ED were enrolled and received cavernosography with 320-row DVCT (DVCT group) and conventional cavernosography scans (control group), respectively. The diagnosis, complications and prognosis of patients were evaluated. The DVCT group provided higher-resolution images with less processing and testing time, as well as lowered does radiological agent and contrast agent compared to the control group...
April 19, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28424370/multi-site-binding-of-epigallocatechin-gallate-to-human-serum-albumin-measured-by-nmr-and-isothermal-calorimetry
#10
Joshua D Eaton, Mike P Williamson
The affinity of epigallocatechin gallate (EGCG) for human serum albumin was measured in physiological conditions using nuclear magnetic resonance (NMR) and isothermal titration calorimetry (ITC). NMR estimated the self-dissociation constant of EGCG as 50 mM. NMR showed two binding events: strong (n1 = 1.8 ± 0.2; Kd1 = 19 ± 12 μM), and weak (n2 ~ 20; Kd2 = 40 ± 20 mM). ITC also showed two binding events: strong (n1 = 2.5 ± 0.03; Kd1 = 21.6 ± 4.0 μM), and weak (n2 = 9 ± 1; Kd2 = 22 ± 4 mM). The two techniques are consistent, with an unexpectedly high number of bound EGCG...
April 19, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28424369/dietary-capsaicin-and-its-anti-obesity-potency-from-mechanism-to-clinical-implications
#11
Jia Zheng, Sheng Zheng, Qianyun Feng, Qian Zhang, Xinhua Xiao
Obesity is a growing public health problem, which has now been considered as a pandemic non-communicable disease. However, the efficacy of several approaches for weight loss is limited and variable. Thus, alternative anti-obesity treatments are urgently warranted, which should be effective, safe and widely available. Active compounds isolated from herbs are similar with the practice of Traditional Chinese Medicine, which has a holistic approach that can targets to several organs and tissues in the whole body...
April 19, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28408433/the-relationship-between-circulating-irisin-levels-and-tissues-age-accumulation-in-type-2-diabetes-patients
#12
Zhu Li, Gang Wang, Yanjuan Zhu, Chenguang Li, Yunzhao Tang, Zhenhuan Jiang, Min Yang, Changlin Ni, Li-Ming Chen, Wen-Yan Niu
Objective: AGEs, measured by skin autofluorescence (AF), are a factor in the development or worsening of many degenerative diseases, such as diabetes and atherosclerosis. Irisin levels has been associated with diabetes, endothelial dysfunction and atherosclerosis. The objective of this study was to investigate whether circulating irisin levels are correlated with skin AF values in type 2 diabetes patients. Design: A total of 362 Chinese type 2 diabetic patients and 100 age- and sex-matched healthy controls were recruited in this study...
April 13, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28404628/simultaneous-detection-of-decidual-th1-th2-and-nk1-nk2-immunophenotyping-in-unknown-recurrent-miscarriage-using-8-color-flow-cytometry-with-fsc-vt-extended-strategy
#13
Peng Dong, Xi Wen, Jia Liu, Cui-Yan Yan, Jing Yuan, Lan-Rong Luo, Qiao-Fei Hu, Jian Li
Th1/Th2 imbalance is considered as a mechanism of recurrent miscarriage. The NK1/NK2 paradigm is hypothesized to play an important role in pregnancy. However, few results showed simultaneous changes of these subsets in vivo in decidual tissues. This study aimed to detect the decidual mononuclear cells and the Th1/Th2 and NK1/NK2 paradigm simultaneously using multiparametric flow cytometry (MFC) in unexplained recurrent miscarriages (URM). Mononuclear cells were isolated from the decidual tissues of URM cases and early pregnant women...
April 12, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28396518/rapamycin-efficiently-promotes-cardiac-differentiation-of-mouse-embryonic-stem-cells
#14
Qin Lu, Zhuqing Jia, Yang Wang, Weiping Wang, Zhe Yang, Tao Li, Yuyao Tian, Ping Chen, Kangtao Ma, Yinan Liu, Chunyan Zhou
To investigate the effects of the rapamycin on cardiac differentiation, murine ESCs was induced to cardiomyocytes by 10(-4) M ascorbic acid (AA), 20 nM rapamycin alone or 0.1‰ solvent dimethyl sulfoxide (DMSO). We found that rapamycin alone was insufficient to initiate cardiomyogenesis. Then the ESCs was treated with AA and rapamycin (20 nM) or AA and DMSO (0.1‰) as a control. Compared with control, mESCs treated with rapamycin (20 nM) and AA yielded a significantly higher percentage of cardiomyocytes, as confirmed by percentage of beating EBs, the immunofluorescence and FACS analysis...
April 10, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28396517/-in-vitrocharacterization-of-six-stub1-variants-in-spinocerebellar-ataxia-16-reveals-altered-structural-properties-for-the-encoded-chip-proteins
#15
Yasaman Pakdaman, Monica Sanchez-Guixé, Rune Kleppe, Sigrid Erdal, Helene J Bustad, Lise Bjørkhaug, Kristoffer Haugarvoll, Charalampos Tzoulis, Ketil Heimdal, Per M Knappskog, Stefan Johansson, Ingvild Aukrust
Autosomal recessive spinocerebellar ataxia-16 is caused by bi-allelic mutations in the STUB1 gene encoding the ubiquitin E3 ligase and dimeric co-chaperone CHIP (C-terminus of Hsc70-Interacting Protein). It has been proposed that the disease mechanism is related to CHIP's impaired E3 ubiquitin ligase properties and/or interaction with its chaperones. However, there is limited knowledge on how these mutations affect the stability, folding and protein structure of CHIP itself. To gain further insight, s ix previously reported pathogenic STUB1 variants (E28K, N65S, K145Q, M211I, S236T and T246M) were expressed as recombinant proteins and studied using limited proteolysis, size-exclusion chromatography and circular dichroism...
April 10, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28396516/root-extractive-from-daphne-genkw-benefits-wounding-healing-of-anal-fistula-through-up-regulation-of-collagen-genes-in-human-skin-fibroblasts
#16
Dong Yang, Junhua Xu, Renjie Shi
The wound healing is the main problem for the therapy of anal fistula (AF). Daphne genkwa ( D. genkwa ) root has been traditionally used as agents to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of this study was to illuminate mechanisms of D. genkwa root treating on AF. In this study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without the D. genkwa extractive. Wounds healing time was compared and granulation tissues were collected...
April 10, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28396515/light-involved-regulation-of-bzr1-stability-andphosphorylation-statusto-coordinate-plant-growth-in-arabidopsis
#17
Qian-Feng Li, Li-Chun Huang, Ke Wei, Jia-Wen Yu, Chang-Quan Zhang, Qiao-Quan Liu
Light and Brassinosteroid (BR) are master environmental stimulus and endogenous cue for plant growth and development, respectively. Great progress has been made in elucidating the molecular mechanisms on the crosstalk between light and BR. However, little is known about how BZR1, the pivotal integration node, is regulated by light and dark. Here, we demonstrated that an intact BR signaling pathway is essential for dark-induced hypocotyl elongation. Consequent expression assay showed that light-dark switch affected BZR1 phosphorylation and accumulation...
April 10, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28396514/effects-ofrhbmp-2gene-transfectionto-periodontal-ligament-cells-on-osteogenesis
#18
Cong-Xiang Jian, Quan-Shui Fan, Yong-He Hu, Yong He, Ming-Zhe Li, Wei-Yin Zheng, Yu Ren, Chen-Jun Li
Objective: This study aims to investigate the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on the osteogenesis of periodontal ligament (PDL) cells. Method: The expression vector of rhBMP-2 (pcDNA3.1-rhBMP-2) was established. PDL cells were obtained through the enzymatic digestion and tissue explant methods and verified by immunohistochemistry. Cells were classified into an experimental (cells were transfected with pcDNA3.1/rhBMP-2-EGFP), blank (cells with no transfection) and control group (cells were transfected with empty plasmid)...
April 10, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28396513/common-polymorphisms-of-the-hogg1-ape1and-xrcc1genescorrelate-with-the-susceptibility-and-clinicopathological-features-of-primary-angle-closure-glaucoma
#19
Kun Zeng, Bo Zhong, Min Fang, Xiao-Li Shen, Li-Na Huang
This case study aims to elucidate the correlation between the hOGG1 , APE1 and XRCC1 gene polymorphisms to the susceptibility and clinicopathological features of primary angle-closure glaucoma (PACG) in a Chinese Han population. Blood samples were obtained from 258 PACG patients (case group) and 272 healthy volunteers (control group). Polymerase chain reaction with sequence specific primer (PCR-SSP) was used to determine the allele frequencies and genotype distributions of the hOGG1 , APE1 and XRCC1 genes. The risk factors of PACG were determined using logistic regression analysis...
April 10, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28396512/pro-inflammatory-cytokine-driven-pi3k-akt-sp1-signaling-and-h2s-production-facilitates-the-pathogenesis-of-severe-acute-pancreatitis
#20
Ying Liu, Ribin Liao, Zhanrong Qiang, Cheng Zhang
Severe acute pancreatitis (SAP) is a disease usually associated with systemic organ dysfunction or pancreatic necrosis. Most patients with SAP suffer from defective intestinal motility in the early phase of the disease. Additionally, SAP-induced inflammation produces hydrogen sulfide (H2S) that impairs the gastrointestinal (GI) system. However, the exact mechanism of H2S in the regulation of SAP is yet to be elucidated. In this paper, we use a rat model of SAP to evaluate the role of H2S on intestinal motility by counting the number of bowel movements and investigating the effect of H2S on inflammation...
April 10, 2017: Bioscience Reports
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