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Bioscience Reports

Bin Yan, Zhiyong Peng, Xiao Xing, Du Chunling
Glibenclamide (Gli) is a widely employed drug in the treatment of type 2 diabetes and many lines of evidence has described its anti-tumoral effects in some neoplasms. The aim of the present study was to investigate the effect of Gli on apoptosis of human hepatocellular carcinoma (HCC) cells and to analyze the underlying pathway involved in this action. Two HCC cell lines, HepG-2 and Huh7 were used as the cell models. We found that Gli treatment significantly inhibited cell viability, induced a significant cell-cycle arrest in G2/M phase and induced apoptosis in both HepG-2 and Huh7 cells...
August 11, 2017: Bioscience Reports
Asha Recino, Kerry Barkan, Graham Ladds, Anne Cooke, F Susan Wong, Maja Wallberg
Metabolism is of central importance to T cell survival and differentiation. It is well known that T cells cannot function in the absence of glucose, but it is less clear how they respond to excessive levels of glucose. In this study we investigated how increasing levels of glucose affect T cell-mediated immune responses. We examined the effects of increased levels of glucose on CD8(+) T cell behaviour in vitro by assessing activation and cytokine production, as well as oxygen consumption rate, extracellular acidification rate and intracellular signalling...
August 10, 2017: Bioscience Reports
Mohammad Ishraq Zafar, Juan Zheng, Wen Kong, Xiaofeng Ye, Luoning Gou, Anita Regmi, Lu-Lu Chen
It has been shown that adipose tissue and skeletal muscles of lean individuals respond to meal-induced hyperinsulinemia by increases in perfusion, the effect not observed in patients with metabolic syndrome. In conditions of hyperglycaemia and hypertriglyceridemia, this insufficient vascularization leads to the liberation of reactive oxygen species, and disruption of nitric oxide synthesis and endothelial signalling responsible for the uptake of circulating fatty acids, whose accumulation in skeletal muscles and adipose tissue is widely associated with impairment of insulin signalling...
August 10, 2017: Bioscience Reports
Kexiang Zhang, Song Wu, Zhiyue Li, Jiahui Zhou
Spinal cord injury (SCI) makes a major contribution to disability and death in the worldwide. Reactive astrogliosis, a typical feature after SCI, which undergoes varying molecular and morphological changes, is ubiquitous but poorly understood. Reactive astrogliosis contributes to glial scar formation that impedes axonal regeneration. Brain-derived Neurotrophic Factor (BDNF), a well-established neurotrophic factor, exerts neuroprotective and growth-promoting effects on a variety of neuronal populations after injury...
August 8, 2017: Bioscience Reports
Amanda Chikaosolu Okolie, Oluwafemi Ezekiel Kale, Odutola Osilesi
Recent studies have shown that Type 2 diabetes (T2D) in rats can result through a synergy that link obesity to insulin resistance and β-cell dysfunction. This present study achieved T2D via high fructose (20%(w/v), p.o.), streptozotocin single dose (40 mg/kg, i.p.) (HFSTZ) in rats. Also, chemoprotective potential of butanol fraction of buchholzia coriacea (BFBC) was demonstrated. Control normal and diabetic untreated (HFSTZ-induced T2D) rats received carboxymethyl cellulose (1 mg/kg, p.o.). Diabetic rats received intragastric BFBC (20, 200, 400 mg/kg), glibenclamide (0...
August 8, 2017: Bioscience Reports
André Luiz Bandeira Dionizio Cardoso, Éric Heleno Freire Ferreira Frederico, Carlos Alberto Sampaio Guimarães, Lívia Pinto Almeida, Rosane de Figueiredo Neves, Danubia da Cunha de Sá-Caputo, Eloá Moreira-Marconi, Carla de Fontoura Dionello, Danielle Soares Morel, Laisa Liane Paineiras-Domingos, Cintia Renata Sousa-Gonçalves, Nasser Ribeiro Asad, Mario Bernardo-Filho
The consequences of the treatment with a natural product and whole body vibration (WBV) exercise have been investigated. The aim of this study was to evaluate the effects of the joint treatment with an aqueous extract of Chenopodium ambrosioides and WBV on physiological parameters in rats. Wistar rats (n=20) were divided equally in four groups: control group (CG), treated with Chenopodium ambrosioides (CHE), exposed to 50 Hz WBV (VBR) and treated with Chenopodium ambrosioides and exposed 50 Hz WBV (VBR+CHE) daily for 6-weeks...
August 7, 2017: Bioscience Reports
Ning Liao, Hua Zhao, Min-Li Chen, Zheng-Fu Xie
It has been hypothesized that polymorphisms in the transforming growth factor-β1 ( TGF-β1 ) gene on chromosome 19 modify the risk for chronic obstructive pulmonary disease (COPD). However, results from previous studies are contradictory. We therefore conducted a meta-analysis of published case-control studies on the association between five common TGF-β1  polymorphisms (rs1982073, rs1800469, rs2241712, rs6957, and rs2241718) and COPD risk. Data sources were Pubmed, Scopus, ISI Web of Science, China National Knowledge Infrastructure (CNKI), and Wanfang databases...
August 7, 2017: Bioscience Reports
Wei Xing, Lei Yang, Yue Peng, Qianlu Wang, Min Gao, Mingshi Yang, Xianzhong Xiao
Background Sepsis-led mitochondrial dysfunction has become a critical pathophysiological procedure of sepsis. Since ginsenosides have been applied in the treatment of mitochondrial dysfunction, ginsenoside Rg3 was employed to study its effects on the mitochondrial dysfunction induced by Sepsis. Experiments The apoptosis rate, oxygen consumption rate (OCR), reactive oxygen species (ROS), antioxidant glutathione (GSH) pools, and mitochondrial transmembrane potential (MTP) were determined in LPS induced sepsis hepatocytes treated with different concentration of Rg3...
August 4, 2017: Bioscience Reports
Guo-Dong Zhen, Lian-Bin Zhao, Shan-Shan Wu, Ming-Yu Chen, Zhen-He Li, Sheng-Zhi Zhou, Zhen-Fu Li
We aim to explore the associations between MMP-2 / MMP-9 gene polymorphism with ulinastatin (UTI) efficacy in treating severe acute pancreatitis (SAP). A total of 276 SAP patients were assigned into the control (n = 135) and observation (n = 141) groups. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for genotype and allele frequency distribution. Relevance of MMP-2 / MMP-9 genotypes with UTI efficacy was analyzed. The observation group showed lowered duration in symptoms (abdominal distension, abdominal pain, tenderness and rebound tenderness) than the control group...
August 4, 2017: Bioscience Reports
Liming Chen, Jianfeng Xu, Xiaoxia Chu, Chenghua Ju
Thrombospondin 1 (TSP1)-producing B cells are an important immune regulatory cell fraction in the body, which are compromised in a number of immune diseases. Micro RNAs (miR) are involved in the immune regulation. This study aims to elucidate the mechanism by which miR-98 interferes with the expression of TSP1 in B cells of the peripheral blood system. In this study, peripheral blood samples were collected from patients with allergic asthma. The B cells were isolated from the blood samples to be analyzed for the expression of miR-98 and TSP1...
July 31, 2017: Bioscience Reports
Laibo Zhang, Zhoubin Tian, Wei Li, Xianquan Wang, Zhentao Man, Shui Sun
Wear particle-induced periprosthetic osteolysis is the main cause of aseptic loosening of orthopaedic implants. The aim of this study is to determine the protective effect of quercetin (QUE) against titanium (Ti) particle-induced ERS-related apoptosis and osteolysis. In this study, RAW264.7 cells were pretreated with different concentrations (40, 80, and 160 μmol/l) of QUE for 30 min and then treated with Ti particle (5 mg/ml) for 24 h. Cell viability and apoptosis were determined using MTT assay and Annexin V-FITC apoptosis detection kit, respectively...
July 31, 2017: Bioscience Reports
Bin Chen, Minguang Zhang, Dongwei Xing, Yu Feng
Background: Hypoxia could enhance radioresistance of prostate cancer cells through up-regulating HIF-1α, which could be inhibited by statins in several cancer cells. However, this effect of statins in prostate cancer remains unclear. In this study, we aim to investigate the effect of atorvastatin on HIF-1αexpression and radiosensitivity of prostate cancers cells.  Methods:The hypoxia-induced human prostate cancer PC3 cells were generated by incubating with 5% O2 for 24 hours. The cell viability and apoptosis were respectively analyzed by cell counting kit-8 (CCK-8) assay and flow cytometry...
July 31, 2017: Bioscience Reports
Aytug Kizilors, Mark R Pickard, Cathleen E Schulte, Kiren Yacqub-Usman, Nicola J McCarthy, Shu-Uin Gan, David Darling, Joop Gäken, Gwyn T Williams, Farzin Farzaneh
The E3 ubiquitin ligase RNF168 is a ring finger protein that has previously been identified to play an important regulatory role in the repair of double-strand DNA breaks.  In the present study, an unbiased forward genetics functional screen in mouse granulocyte/ macrophage progenitor cell line FDCP1 has identified E3 ubiquitin ligase RNF168 as a key regulator of cell survival and proliferation. Our data indicate that RNF168 is an important component of the mechanisms controlling cell fate, not only in human and mouse haematopoietic growth factor-dependent cells, but also in the human breast epithelial cell line MCF-7...
July 27, 2017: Bioscience Reports
Dong Keun Rhee, Steven C Hockman, Sun-Kyung Choi, Yong-Eun Kim, Chungoo Park, Vincent C Manganiello, Kee Kwang Kim
Phosphodiesterase 3A (PDE3A), a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (PDE) family, plays important roles in oocyte maturation and vascular smooth muscle cell proliferation. However, the molecular mechanisms that regulate PDE3A gene expression remain largely unknown. In this study, we investigated the transcriptional regulation of PDE3A , and found that the splicing factor proline and glutamine rich (SFPQ) protein modulated PDE3A mRNA levels. Multiple transcription start sites (TSS1, 2, and 3) were identified within the first exon of PDE3A using 5'-rapid amplification of cDNA ends (RACE)...
July 25, 2017: Bioscience Reports
Da Chen, Fangming Zhong, Ye Chen
Calcium/calmodulin-dependent protein kinase kinase1 (CAMKK1) could specially recognize and activate Calcium/calmodulin-dependent protein kinase (CAMK) I and IV. Furthermore, The activation of CAMK showed positively correlation with proliferation of lung cancer (LC). In addition, A genome-wide association study has identified rs7214723 (E375G) in the CAMKK1 gene as a susceptibility locus for LC in the UK population. Therefore, we conducted a case-control study involving 320 LC patients and 320 controls to validate this conclusion in a Chinese population...
July 24, 2017: Bioscience Reports
Astrid Kehlen, Monique Haegele, Livia Böhme, Holger Cynis, Torsten Hoffmann, Hans-Ulrich Demuth
CX3CL1 is the unique member of the CX3C chemokine family and mediates both adhesion and cell migration in inflammatory processes. Frequently, the activity of chemokines depends on a modified N-terminus as described for the N-terminus of CCL2 modified to a pyroglutamate (pGlu-) residue by glutaminyl cyclase activity. Here, we assess the role of the pGlu-modified residue of the CX3CL1 chemokine domain in human endothelial and smooth muscle cells. For the first time, we demonstrated using mass spectrometry that glutaminyl cyclase (QC, QPCT ) or its isoenzyme isoQC ( QPCTL ) catalyse the formation of N-terminal modified pGlu-CX3CL1...
July 24, 2017: Bioscience Reports
Zhi-Kui Wang, Zhao-Xia Wang, Zhen-Ying Liu, Yue-Qin Ren, Zhong-Qi Zhou
We investigated the effects of RNA interference-mediated gene silencing of VEGF on ultrafiltration failure (UFF) in rats with peritoneal dialysis (PD). SD male rats were classified into normal, sham operation, and uremic model groups. Uremic rats were sub-categorized into uremia, PD2, VEGF shRNA-2, vector-2, PD2 + Endostar, PD4, VEGF shRNA-4, Vector-4 and PD4 + Endostar groups. Peritoneal Equilibration Test (PET) was conducted to assess ultrafiltration volume (UFV) and mass transfer of glucose (MTG). mRNA and protein expressions of VEGF were detected using qRT-PCR and Western blotting...
July 21, 2017: Bioscience Reports
Yea Woon Kim, AeRi Kim
Transcription factors play roles in gene transcription through direct binding to their motifs in genome, and inhibiting this binding provides an effective strategy for studying their roles. Here we applied the CRISPR/spCas9 system to mutate the binding motifs of transcription factors. Binding motifs for erythroid specific transcription factors were mutated in the locus control region hypersensitive sites of the human β-globin locus. Guide RNAs targeting binding motifs were cloned into lentiviral CRISPR vector containing the spCas9 gene, and transduced into MEL/ch11 cells carrying a human chromosome 11...
July 20, 2017: Bioscience Reports
Henrick Horita, Andy Law, Soonjin Hong, Kim Middleton
Identification of a novel post-translational modification (PTM) for a target protein, defining its physiologic role, and studying its potential crosstalk with other PTMs is a challenging process. A set of highly sensitive tools termed Signal-Seeker kits was developed, which enables rapid and simple detection of post-translational modifications on any target protein.  The methodology for these tools utilizes affinity purification of modified proteins from a cell or tissue lysate and immunoblot analysis. These tools utilize a single lysis system that is effective at identifying endogenous, dynamic PTM changes, as well as the potential crosstalk between PTMs...
July 19, 2017: Bioscience Reports
Jiu-Jiang Wang, Zhi-Feng Li, Xiao-Jing Li, Zhao Han, Ling Zhang, Zhi-Jun Liu
The study aims to evaluate the effects of miR-136 on the proliferation, apoptosis and epithelial mesenchymal transition (EMT) of melanoma cells by targeting PMEL through the Wnt signaling pathway. After establishment of melanoma mouse models, melanoma (model group) and normal tissues (normal group) were collected. Immunohistochemistry was performed to determine PMEL protein concentration. Mouse melanoma cells were assigned into control, blank, negative control (NC), miR-136 mimics, miR-136 inhibitors, siRNA-PMEL, and miR-136 inhibitors + siRNA-PMEL, LiC1 (Wnt signaling pathway activator) and siRNA-PMEL+ LiCl groups...
July 19, 2017: Bioscience Reports
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