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Veterinary Research Communications

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https://www.readbyqxmd.com/read/29340849/risk-factors-associated-with-feline-urolithiasis
#1
REVIEW
Veridiane da Rosa Gomes, Paula Costa Ariza, Naida Cristina Borges, Francisco Jorge Schulz, Maria Clorinda Soares Fioravanti
Urinary tract diseases are among the main reasons for consultation in veterinary clinics and hospitals. It affects animals of any age, breed and gender. Among the diseases that affect this system, urolithiasis is the second largest cause of clinical signs compatible with feline urinary tract disease. The term urolithiasis refers to the presence of uroliths in any region of the urinary tract, but it is more commonly seen in the bladder and urethra. Uroliths are classified based on the type of mineral present in their composition, therefore, quantitative and qualitative analyzes are important for a better therapeutic approach...
January 16, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29302913/principles-and-applications-of-the-balanced-steady-state-free-precession-sequence-in-small-animal-low-field-mri
#2
Mario Ricciardi
Magnetic resonance imaging (MRI) in small animal practice is largely based on classic two-dimensional spin-echo, inversion recovery and gradient-echo sequences which are largely limited by low spatial resolution, especially in low-field (LF)-MRI scanners. Nowadays, however, the availability of volumetric sequences can open new perspectives and enhance the diagnostic potential of this imaging modality. Balanced steady-state free precession (bSSFP) is a three-dimensional gradient-echo sequence in which image contrast is given by the ratio of T2 and T1, resulting in low soft-tissue signal, poor cerebral grey/white matter distinction and a bright signal from free fluid and fat...
January 4, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29297135/stem-cell-factor-supports-migration-in-canine-mesenchymal-stem-cells
#3
Nathaly Enciso, Luciana L K Ostronoff, Guillermo Mejías, Leticia G León, María Luisa Fermín, Elena Merino, Cristina Fragio, Luis Avedillo, Concepción Tejero
Adult Mesenchymal Stem Cells (MSC) are cells that can be defined as multipotent cells able to differentiate into diverse lineages, under appropriate conditions. These cells have been widely used in regenerative medicine, both in preclinical and clinical settings. Initially discovered in bone marrow, MSC can now be isolated from a wide spectrum of adult and foetal tissues. Studies to evaluate the therapeutic potential of these cells are based on their ability to arrive to damaged tissues. In this paper we have done a comparative study analyzing proliferation, surface markers and OCT4, SOX9, RUNX2, PPARG genes expression in MSC cells from Bone marrow (BMMSC) and Adipose tissue (ASC)...
January 3, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29297134/tamoxifen-induces-apoptotic-neutrophil-efferocytosis-in-horses
#4
C Olave, N Morales, B Uberti, C Henriquez, J Sarmiento, A Ortloff, H Folch, G Moran
Macrophages and neutrophils are important cellular components in the process of acute inflammation and its subsequent resolution, and evidence increasingly suggests that they play important functions during the resolution of chronic, adaptive inflammatory processes. Exacerbated neutrophil activity can be harmful to surrounding tissues; this is important in a range of diseases, including allergic asthma and chronic obstructive pulmonary disease in humans, and equine asthma (also known as recurrent airway obstruction (RAO)...
January 2, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29285579/r132-mutations-in-canine-isocitrate-dehydrogenase-1-idh1-lead-to-functional-changes
#5
Shota Kawakami, Kazuhiko Ochiai, Daigo Azakami, Yuiko Kato, Masaki Michishita, Masami Morimatsu, Toshina Ishiguro-Oonuma, Eri Onozawa, Masami Watanabe, Toshinori Omi
Glioma is the second most common intracranial neoplasia in dogs, but the pathogenic mechanisms remain unclear. In humans, isocitrate dehydrogenase 1 (IDH1) is frequently mutated in gliomas. Although almost all human IDH1 mutations have been identified as involving the Arg132 codon, few studies have reported structural, functional, and mutational information for canine IDH1. Therefore, in this study, we cloned the canine IDH1 homologue and used PCR mutagenesis to substitute the wildtype (WT) Arg132 with His (R132H) or Ser (R132S)...
December 29, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/29280084/the-effect-of-nesfatin-1-on-food-intake-in-neonatal-chicks-role-of-crf1-crf2-and-h1-h3-receptors
#6
Hooman Heidarzadeh, Morteza Zendehdel, Vahab Babapour, Hasan Gilanpour
The present study was designed to determine the effect of central injection of Nesfatin-1 and corticotropin and histaminergic systems on food intake in neonatal meat-type chicks. In this study, 7 experiments were designed, each with 4 treatment groups. In experiment 1, four groups of chicks received the ICV injection of (A) phosphate-buffered saline (PBS), (B) Nesfatin-1 (10 ng), (C) Nesfatin-1 (20 ng) and (D) Nesfatin-1 (40 ng). In experiment 2, (A) PBS, (B) Astressin-B (CRF1/CRF2 receptors antagonist; 30 µg), (C) Nesfatin-1 (40 ng) and (D) Nesfatin-1 + Astressin-B were injected...
December 26, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/29222615/knockdown-expression-of-il-10r%C3%AE-gene-inhibits-prrsv-replication-and-elevates-immune-responses-in-pbmcs-of-tibetan-pig-in-vitro
#7
Yihui Chen, Ye Wang, Kai Zeng, Yun-Feng Lei, Xiao-Hui Chen, San-Cheng Ying, Xue-Bin Lv, Zezhou Wang, Rong Gao
Increase of interleukin-10 (IL-10) induced by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection has been intensely studied to inhibit the anti-viral responses of host pigs. Blockade of expression of IL-10 receptor (IL-10R) by RNA interference (RNAi) may relieve the immunosuppression caused by excessive IL-10 in PRRSV infection. The recombinant short hairpin expressing plasmid targeted to pig IL-10Rα was transfected into peripheral blood mononuclear cells of Tibetan pig (Tp-PBMCs) prior to PRRSV inoculation, then the replication of PRRSV and immune responses in Tp-PBMCs were evaluated...
December 8, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/29204821/development-of-a-clinical-prediction-score-for-detection-of-suspected-cases-of-equine-grass-sickness-dysautonomia-in-france
#8
P K Randleff-Rasmussen, A Leblond, J Cappelle, J Bontemps, S Belluco, M R Popoff, C Marcillaud-Pitel, J Tapprest, P Tritz, I Desjardins
Equine grass sickness (EGS) (equine dysautonomia) is a neurodegenerative condition of grazing equines. Pre-mortem diagnosis of EGS is a challenge for practitioners as definitive diagnosis requires ileal/myenteric lymph node biopsies. This study aimed to develop a clinical score that could be used by practitioners to improve the detection of acute or subacute EGS cases in the field. Suspected EGS cases were declared by veterinary practitioners. A case was classified as confirmed positive if ileal or rectal biopsy samples showed neuronal degeneration typical of EGS...
December 4, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/29177583/identification-and-functional-analysis-of-the-novel-orf6-protein-of-porcine-circovirus-type-2-in-vitro
#9
Dongge Li, Jing Wang, Shangen Xu, Shanxia Cai, Chaojie Ao, Liurong Fang, Shaobo Xiao, Huanchun Chen, Yunbo Jiang
In the present study, the function of a novel ORF6 gene in the PCV2 genome was determined and functionally analyzed in vitro. ORF6 expression was demonstrated by indirect immunofluorescence in PCV2-infected cells. The antibody against ORF6 was detected in PCV2-infected pigs. The start codon of ORF6 was mutated and an infectious clone was used to create an ORF6-deficient mutant virus. Viral DNA replication curves and immunofluorescence analysis indicated that ORF6 is unnecessary for viral replication and ORF6 deletion reduces viral DNA replication in PK-15 cells...
November 25, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/29119302/goldfish-carassius-auratus-as-an-infection-model-for-studying-the-pathogenesis-of-edwardsiella-piscicida
#10
Yunjeong Choe, Jong Earn Yu, Junmo Park, Dongchul Park, Jeong-Il Oh, Suhkmann Kim, Ki Hwan Moon, Ho Young Kang
This study demonstrates the feasibility of using goldfish as an infection model to investigate the pathogenesis of Edwardsiella piscicida. Goldfish were found to be susceptible to acute E. piscicida-induced disease and died in a dose-dependent manner. E. piscicida was further shown to replicate rapidly in the head kidneys and livers of infected goldfish from 1 d post-injection, and bacteria numbers were significantly decreased 5 d post-injection. Immune responses were successfully induced in goldfish injected with E...
December 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/29098532/microbial-community-and-ovine-host-response-varies-with-early-and-late-stages-of-haemonchus-contortus-infection
#11
Saeed El-Ashram, Ibrahim Al Nasr, Fathi Abouhajer, Maged El-Kemary, Guangping Huang, Güngör Dinçel, Rashid Mehmood, Min Hu, Xun Suo
The interactions between gastric microbiota, ovine host, and Haemonchus contortus portray the ovine gastric environment as a complex ecosystem, where all factors play a pertinent role in fine-tuning each other and in haemeostasis. We delineated the impact of early and late Haemonchus infection on abomasal and ruminal microbial community, as well as the ovine host. Twelve, parasite-naive lambs were divided into four groups, 7 days post-infection (dpi) and time-matched uninfected-control groups; 50 dpi and time-matched uninfected control groups were used for the experiment...
December 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/29081026/2-3-7-8-tetrachlorodibenzo-p-dioxin-influences-bovine-herpesvirus-1-replication-through-upregulation-of-sirt3-and-cytoskeletal-reorganization
#12
Filomena Fiorito, Valentina Iovane, Annarosaria Marullo, Anna Costagliola, Giovanna Elvira Granato, Luisa De Martino
Infection of kidney cells (MDBK) with Bovine Herpesvirus 1 (BoHV-1) is affected by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which accelerates BoHV-1-induced apoptosis and increases virus replication. Herein, to elucidate the mechanism through TCDD modifies BoHV-1 infection, we analyzed the modulation of a members of Sirtuin proteins family in MDBK cells. We found that mitochondrial SIRT3 was upregulated during infection. This change was accompanied by cytoskeletal rearrangements and cell extensions. All these trends were drastically modified by TCDD...
December 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28942490/susceptibility-of-mice-to-bovine-herpesvirus-type-5-infection-in-the-central-nervous-system
#13
L P Mesquita, R C Costa, M M Fusuma, F R P Bruhn, E Mori, E M Pituco, C M C Mori, R Weiblen, P C Maiorka
Bovine herpesvirus type 5 (BoHV-5) is an important pathogen that causes meningoencephalitis in cattle. Few studies have used the mouse as a model for BoHV-5 infection. Despite the fact that BoHV-5 can infect mice with immune deficiencies, little is known about viral replication, immune response, and the course of infection in the central nervous system (CNS) of wild-type mice. Therefore, the aim of this study was to evaluate the response in the CNS of BALB/c mice acutely infected with BoHV-5 at different days post-inoculation (dpi)...
December 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28842847/visual-adaptations-of-the-eye-of-the-gilthead-sea-bream-sparus-aurata
#14
Neveen E R El-Bakary, Mohamed M A Abumandour
The current investigation represents the morphological description of the adaptation of gilthead sea bream Sparus aurata to its environmental conditions. For the achievement of this study twelve mature fishes were used for gross, light and electron microscope examinations. The cornea is consists of three layers; an anterior stratified cuboidal epithelium, bowman layer, and a dermal stroma. A mucoid layer located between the dermal stroma and the anterior part of the scleral cornea, while the iridescent layer located between the anterior and the posterior scleral stroma...
December 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28836073/a-ten-years-2007-2016-retrospective-serological-survey-for-seneca-valley-virus-infection-in-major-pig-producing-states-of-brazil
#15
Viviane Saporiti, Juliana T T Fritzen, Cesar Feronato, Raquel A Leme, Zelia I P Lobato, Alice F Alfieri, Amauri A Alfieri
Seneca Valley virus (SVV) is the etiological agent of vesicular disease in pigs, clinically indistinguishable of classical viral vesicular infections, including foot-and-mouth disease. The first outbreaks of SVV infection in Brazil were reported in 2014. However, it was not known whether the virus was circulating in Brazilian pig herds before this year. This study is a retrospective serological investigation of porcine health status to SVV in Brazil. Serum samples (n = 594) were grouped in before (2007-2013, n = 347) and after (2014-2016, n = 247) SVV outbreaks in Brazil...
December 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28791606/vasovagal-tonus-index-vvti-as-an-indirect-assessment-of-remission-status-in-canine-multicentric-lymphoma-undergoing-multi-drug-chemotherapy
#16
Evi Pecceu, Brittainy Stebbing, Yolanda Martinez Pereira, Ian Handel, Geoff Culshaw, Hannah Hodgkiss-Geere, Jessica Lawrence
Vasovagal tonus index (VVTI) is an indirect measure of heart rate variability and may serve as a marker of disease severity. Higher heart rate variability has predicted lower tumour burden and improved survival in humans with various tumour types. The purpose of this pilot study was to evaluate VVTI as a biomarker of remission status in canine lymphoma. The primary hypothesis was that VVTI would be increased in dogs in remission compared to dogs out of remission. Twenty-seven dogs were prospectively enrolled if they had a diagnosis of intermediate to high-grade lymphoma and underwent multidrug chemotherapy...
December 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28634673/b-mode-and-doppler-ultrasound-of-chronic-kidney-disease-in-dogs-and-cats
#17
REVIEW
Nathália Bragato, Naida Cristina Borges, Maria Clorinda Soares Fioravanti
Ultrasound is the imaging test of choice for renal evaluation, because it provides information about the position, size, shape, internal architecture and hemodynamics of the kidneys without harming the patient. In chronic kidney disease, the main findings observed in B-mode ultrasound images are increased cortical echogenicity, loss of corticomedullary differentiation, reduced renal volume and irregular renal contour, and when these changes are associated, they are indicative of end-stage renal disease. However, the cause of kidney disease cannot be determined by ultrasonography, but must be confirmed by means of biopsy, although the presence of ultrasonographic changes indicative of the end-stage of the disease may contraindicate this procedure...
December 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28589422/modulatory-function-of-nmda-glutamate-receptor-on-mc3-mc4-receptors-agonist-induced-hypophagia-in-neonatal-meat-type-chicken
#18
Fariba Ahmadi, Morteza Zendehdel, Vahab Babapour, Negar Panahi, Shahin Hassanpour, Mina Khodadadi
Melanocortin 3 and 4 receptors (MC3R and MC4R) are known as the main receptors for melanocortin-induced hypophagia in mammalian and poultry. Also, central glutamatergic system has mediatory role on function of the melanocortin system in some brain areas. So, the aim of the current study was to determine the role of MC3/MC4 receptors agonist on food intake and its interaction with glutamatergic in 3-h food-deprived (FD3) neonatal broilers. In experiment 1, chickens were intracerebroventricular (ICV) injected with control solution, MTII (MC3/MC4 receptors agonist; 2...
December 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28589421/tat-myod-fused-proteins-together-with-c2c12-conditioned-medium-are-able-to-induce-equine-adult-mesenchimal-stem-cells-towards-the-myogenic-fate
#19
Marco Patruno, Chiara Gomiero, Roberta Sacchetto, Ohad Topel, Alessandro Negro, Tiziana Martinello
The Tat protein is able to translocate through the plasma membrane and when it is fused with other peptides may acts as a protein transduction system. This ability appears particularly interesting to induce tissue-specific differentiation when the Tat protein is associated to transcription factors. In the present work, the potential of the complex Tat-MyoD in inducing equine peripheral blood mesenchymal stem cells (PB-MSCs) towards the myogenic fate, was evaluated. Results showed that the internalization process of Tat-MyoD happens only in serum free conditions and that the nuclear localization of the fused complex is observed after 15 hours of incubation...
September 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28573410/depressed-polymorphonuclear-cell-functions-in-periparturient-cows-that-develop-postpartum-reproductive-diseases
#20
Rafiqul Islam, Harendra Kumar, Gyanendra Singh, Binsila B Krishnan, Sahadeb Dey
The study was planned to see if there is any important and significant changes in the PMN function in cows suffering from postpartum reproductive diseases (PRD). Blood sampling was done from 41 pregnant cows on 15 days prepartum (-15d), calving day (0d), 15 days (15d) and 30 days (30d) postpartum and thorough gynaecological examination was performed on 0d, 15d, 30d and 45d for diagnosis of PRD like retained placenta (RP), clinical metritis (CM), clinical endometritis (CE) and delayed involution of uterus (DIU)...
September 2017: Veterinary Research Communications
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