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Veterinary Research Communications

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https://www.readbyqxmd.com/read/29790058/the-synergistic-effect-of-organic-acids-phytochemicals-and-a-permeabilizing-complex-reduces-salmonella-typhimurium-1-4-5-12-i-shedding-in-pigs
#1
Ruggeri Jessica, Foresti Fabio, Pavesi Roberta, Terrini Alessia, Giudici Francesca, Padoan Diego, Corradi Attilio, Ossiprandi Maria Cristina, Pasquali Paolo, Alborali Giovanni Loris
Salmonella Typhimurium (including S.Typhimurium 1,4,[5],12:i-) and other enteric pathogens cause acute infection in pigs during the weaning stage, often evolving into chronic infections responsible for the introduction of zoonotic bacteria into the slaughterhouse and thus determining carcass contamination. In addition to being zoonotic hazards, these pathogens are responsible for economic losses in affected farms. Traditionally, antibiotic treatments have been largely administered in order to reduce the infection burden but it favored, as a direct consequence, an increase in the number of multi-drug resistance strains...
May 22, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29777375/a-comparison-of-the-effects-of-antibiotics-probiotics-synbiotics-and-prebiotics-on-the-performance-and-carcass-characteristics-of-broilers
#2
Vahid Tayeri, Alireza Seidavi, Leila Asadpour, Clive J C Phillips
Routine use of the antibiotic flavomycin in broiler production may lead to resistance, and alternative growth promoters are used to enhance performance. Two hundred day-old male Ross 308 broiler chicks were allocated to five dietary supplements included from d 1-42: flavomycin, three possible alternatives, a probiotic, prebiotic and a synbiotic, as well as a control treatment. There were four replicate cages of 10 birds each in each treatment. Compared with the control and antibiotics treatments, the probiotic, prebiotic and synbiotic treatments increased (p = 0...
May 18, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29721754/effects-of-three-dimensional-spheroid-culture-on-equine-mesenchymal-stem-cell-plasticity
#3
Mi Jeong Park, Jienny Lee, Jeong Su Byeon, Da-Un Jeong, Na-Yeon Gu, In-Soo Cho, Sang-Ho Cha
Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy fields. We optimize culture conditions of equine adipose tissue-derived MSCs (eAD-MSCs) for treatment of horse fractures. To investigate enhancing properties of three-dimensional (3D) culture system in eAD-MSCs, we performed various sized spheroid formation and determined changes in gene expression levels to obtain different sized spheroid for cell therapy. eAD-MSCs were successfully isolated from horse tailhead. Using hanging drop method, spheroid formation was generated for three days...
May 2, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29700691/-nano-immuno-test-for-the-detection-of-live-mycobacterium-avium-subspecies-paratuberculosis-bacilli-in-the-milk-samples-using-magnetic-nano-particles-and-chromogen
#4
Manju Singh, Shoor Vir Singh, Saurabh Gupta, Kundan Kumar Chaubey, Bjorn John Stephan, Jagdip Singh Sohal, Manali Dutta
Early rapid detection of Mycobacterium avium subspecies paratuberculosis (MAP) bacilli in milk samples is the major challenge since traditional culture method is time consuming and laboratory dependent. We report a simple, sensitive and specific nano-technology based 'Nano-immuno test' capable of detecting viable MAP bacilli in the milk samples within 10 h. Viable MAP bacilli were captured by MAP specific antibody-conjugated magnetic nano-particles using resazurin dye as chromogen. Test was optimized using true culture positive (10-bovine and 12-goats) and true culture negative (16-bovine and 25-goats) raw milk samples...
April 26, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29637458/correction-to-plasma-alpha-tocopherol-determined-by-hplc-in-dogs-at-different-stages-of-chronic-kidney-disease-a-retrospective-study
#5
Ilaria Lippi, F Perondi, V Meucci, C Mannarini, L Intorre, G Guidi
The above article originally published with an error present in the article title, "Plasma alpha-tochopherol determined by HPLC in dogs at different stages of chronic kidney disease: a retrospective study" this should instead have read, "Plasma alpha-tocopherol determined by HPLC in dogs at different stages of chronic kidney disease: a retrospective study" [bold text used to highlight problem area].
April 11, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29619666/effectiveness-of-different-avian-influenza-h5-vaccination-regimens-in-layer-chickens-on-the-humoral-immune-response-and-interferon-alpha-signalling-immune-marker
#6
Mustafa Hamad, Omar Amen, Mohamed Mahmoud, Ola Hassanin, Mostafa Saif-Edin
Avian influenza (AI) vaccines are widely used to control and eliminate the ongoing avian influenza virus epidemic in Egypt. A strict vaccination policy with inactivated AI vaccines has been widely applied, however the virus still circulating, evolving and causing great negative impact to the poultry sector in Egypt. Therefore, an updated poultry vaccination policy using different vaccine technologies might be valuable as an innovative additional control strategy of AIV in Egypt. In the present study, the effectiveness of different avian influenza (AI) vaccination schedules was evaluated in 300 commercial layer chicks (ISA White) using either the oil-emulsion baculovirus-H5-prototype vaccine (baculovirus-H5 prototype) or turkey herpesvirus (HVT) vector vaccine containing the hemagglutinin (HA) gene from H5N1 strain (rHVT-H5), applied alone or in combination and in different settings...
April 4, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29582226/a-long-term-feed-supplementation-based-on-phosphate-binders-in-feline-chronic-kidney-disease
#7
E Biasibetti, E Martello, M Bigliati, I Biasato, T Cocca, N Bruni, M T Capucchio
Chronic kidney disease (CKD) is a very common disorder in elderly cats. A proper renal diet represents the most efficient therapeutic intervention to improve survival and life quality in feline patients with 3 and 4 International Renal Interest Society (IRIS) stages. Twenty cats were selected in this study. Ten were administered the dietary supplementation for 360 days and the other ten, whose owners did not give consent for any supplemental therapies apart from the renal diet, were selected from a clinical database and used as control group...
March 26, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29541992/b-mode-ultrasound-examination-of-canine-mammary-gland-neoplastic-lesions-of-small-size-diameter-2-cm
#8
Iacopo Vannozzi, Matteo Tesi, Marta Zangheri, Viola Maria Innocenti, Alessandra Rota, Simonetta Citi, Alessandro Poli
Ultrasonography is a valuable tool for the evaluation of neoplastic lesions in the dog and there is a growing interest in the use of this technique for the stadiation of canine mammary tumours. An accurate assessment of small sized nodules facilitates the stadiation of the mammary lesions and helps the clinician in the choice of the most indicated surgical therapy. The aim of this study was to identify those ultrasound criteria that may be useful in discriminating between benign and malignant lesions of small size (diameter smaller than 2 cm)...
June 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29536334/polymorphism-coupling-interval-and-prematurity-index-in-dogs-with-degenerative-mitral-valve-disease-and-ventricular-arrhythmias
#9
Elizabeth Regina Carvalho, Roberto Andrés Navarrete Ampuero, Giovana Laís Ruviaro Tuleski, Aparecido Antonio Camacho, Marlos Gonçalves Sousa
Ventricular arrhythmias (VA) are a recognized concern in dogs with degenerative mitral valve disease (DMVD). The coupling interval (CI) and the prematurity index (PI) have been shown to accurately differentiate between benign and malignant VA in people, where ventricular arrhythmias are known to be associated with an increased risk of development of signs of heart failure or sudden death. In this study, we characterized ventricular arrhythmias in dogs with symptomatic and asymptomatic DMVD. Seventy dogs with naturally-occurring DMVD and ventricular arrhythmias were retrospectively studied...
June 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29302913/principles-and-applications-of-the-balanced-steady-state-free-precession-sequence-in-small-animal-low-field-mri
#10
REVIEW
Mario Ricciardi
Magnetic resonance imaging (MRI) in small animal practice is largely based on classic two-dimensional spin-echo, inversion recovery and gradient-echo sequences which are largely limited by low spatial resolution, especially in low-field (LF)-MRI scanners. Nowadays, however, the availability of volumetric sequences can open new perspectives and enhance the diagnostic potential of this imaging modality. Balanced steady-state free precession (bSSFP) is a three-dimensional gradient-echo sequence in which image contrast is given by the ratio of T2 and T1, resulting in low soft-tissue signal, poor cerebral grey/white matter distinction and a bright signal from free fluid and fat...
March 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29297135/stem-cell-factor-supports-migration-in-canine-mesenchymal-stem-cells
#11
Nathaly Enciso, Luciana L K Ostronoff, Guillermo Mejías, Leticia G León, María Luisa Fermín, Elena Merino, Cristina Fragio, Luis Avedillo, Concepción Tejero
Adult Mesenchymal Stem Cells (MSC) are cells that can be defined as multipotent cells able to differentiate into diverse lineages, under appropriate conditions. These cells have been widely used in regenerative medicine, both in preclinical and clinical settings. Initially discovered in bone marrow, MSC can now be isolated from a wide spectrum of adult and foetal tissues. Studies to evaluate the therapeutic potential of these cells are based on their ability to arrive to damaged tissues. In this paper we have done a comparative study analyzing proliferation, surface markers and OCT4, SOX9, RUNX2, PPARG genes expression in MSC cells from Bone marrow (BMMSC) and Adipose tissue (ASC)...
March 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29297134/tamoxifen-induces-apoptotic-neutrophil-efferocytosis-in-horses
#12
C Olave, N Morales, B Uberti, C Henriquez, J Sarmiento, A Ortloff, H Folch, G Moran
Macrophages and neutrophils are important cellular components in the process of acute inflammation and its subsequent resolution, and evidence increasingly suggests that they play important functions during the resolution of chronic, adaptive inflammatory processes. Exacerbated neutrophil activity can be harmful to surrounding tissues; this is important in a range of diseases, including allergic asthma and chronic obstructive pulmonary disease in humans, and equine asthma (also known as recurrent airway obstruction (RAO)...
March 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29285579/r132-mutations-in-canine-isocitrate-dehydrogenase-1-idh1-lead-to-functional-changes
#13
Shota Kawakami, Kazuhiko Ochiai, Daigo Azakami, Yuiko Kato, Masaki Michishita, Masami Morimatsu, Toshina Ishiguro-Oonuma, Eri Onozawa, Masami Watanabe, Toshinori Omi
Glioma is the second most common intracranial neoplasia in dogs, but the pathogenic mechanisms remain unclear. In humans, isocitrate dehydrogenase 1 (IDH1) is frequently mutated in gliomas. Although almost all human IDH1 mutations have been identified as involving the Arg132 codon, few studies have reported structural, functional, and mutational information for canine IDH1. Therefore, in this study, we cloned the canine IDH1 homologue and used PCR mutagenesis to substitute the wildtype (WT) Arg132 with His (R132H) or Ser (R132S)...
March 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29280084/the-effect-of-nesfatin-1-on-food-intake-in-neonatal-chicks-role-of-crf-1-crf2-and-h1-h3-receptors
#14
Hooman Heidarzadeh, Morteza Zendehdel, Vahab Babapour, Hasan Gilanpour
The present study was designed to determine the effect of central injection of Nesfatin-1 and corticotropin and histaminergic systems on food intake in neonatal meat-type chicks. In this study, 7 experiments were designed, each with 4 treatment groups. In experiment 1, four groups of chicks received the ICV injection of (A) phosphate-buffered saline (PBS), (B) Nesfatin-1 (10 ng), (C) Nesfatin-1 (20 ng) and (D) Nesfatin-1 (40 ng). In experiment 2, (A) PBS, (B) Astressin-B (CRF1 /CRF2 receptors antagonist; 30 µg), (C) Nesfatin-1 (40 ng) and (D) Nesfatin-1 + Astressin-B were injected...
March 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29222615/knockdown-expression-of-il-10r%C3%AE-gene-inhibits-prrsv-replication-and-elevates-immune-responses-in-pbmcs-of-tibetan-pig-in-vitro
#15
Yihui Chen, Ye Wang, Kai Zeng, Yun-Feng Lei, Xiao-Hui Chen, San-Cheng Ying, Xue-Bin Lv, Zezhou Wang, Rong Gao
Increase of interleukin-10 (IL-10) induced by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection has been intensely studied to inhibit the anti-viral responses of host pigs. Blockade of expression of IL-10 receptor (IL-10R) by RNA interference (RNAi) may relieve the immunosuppression caused by excessive IL-10 in PRRSV infection. The recombinant short hairpin expressing plasmid targeted to pig IL-10Rα was transfected into peripheral blood mononuclear cells of Tibetan pig (Tp-PBMCs) prior to PRRSV inoculation, then the replication of PRRSV and immune responses in Tp-PBMCs were evaluated...
March 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29204821/development-of-a-clinical-prediction-score-for-detection-of-suspected-cases-of-equine-grass-sickness-dysautonomia-in-france
#16
P K Randleff-Rasmussen, A Leblond, J Cappelle, J Bontemps, S Belluco, M R Popoff, C Marcillaud-Pitel, J Tapprest, P Tritz, I Desjardins
Equine grass sickness (EGS) (equine dysautonomia) is a neurodegenerative condition of grazing equines. Pre-mortem diagnosis of EGS is a challenge for practitioners as definitive diagnosis requires ileal/myenteric lymph node biopsies. This study aimed to develop a clinical score that could be used by practitioners to improve the detection of acute or subacute EGS cases in the field. Suspected EGS cases were declared by veterinary practitioners. A case was classified as confirmed positive if ileal or rectal biopsy samples showed neuronal degeneration typical of EGS...
March 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29177583/identification-and-functional-analysis-of-the-novel-orf6-protein-of-porcine-circovirus-type-2-in-vitro
#17
Dongge Li, Jing Wang, Shangen Xu, Shanxia Cai, Chaojie Ao, Liurong Fang, Shaobo Xiao, Huanchun Chen, Yunbo Jiang
In the present study, the function of a novel ORF6 gene in the PCV2 genome was determined and functionally analyzed in vitro. ORF6 expression was demonstrated by indirect immunofluorescence in PCV2-infected cells. The antibody against ORF6 was detected in PCV2-infected pigs. The start codon of ORF6 was mutated and an infectious clone was used to create an ORF6-deficient mutant virus. Viral DNA replication curves and immunofluorescence analysis indicated that ORF6 is unnecessary for viral replication and ORF6 deletion reduces viral DNA replication in PK-15 cells...
March 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29464589/detection-of-trypanosoma-vivax-dna-in-semen-from-experimentally-infected-goats
#18
Nicholas Morais Bezerra, Gabriela Hémylin Ferreira Moura, Hélio Noberto de Araújo, Francisco Silvestre Brilhante Bezerra, Kaliane Alessandra Rodrigues de Paiva, Kizzy Millenn de Freitas Mendonça Costa, Wirton Peixoto Costa, Dayse Ariane Soares Medeiros, Jael Soares Batista
The present work aimed to investigate the presence of T. vivax DNA in the semen of experimentally infected goats. Twelve male goats native to the Brazilian Northeast, adults, were randomly assigned to two experimental groups: the infected group consisting of six goats infected intravenously with 0.5 mL of blood containing approximately 1.25 × 105 trypomastigotes of T. vivax, and a control group composed of six uninfected goats. After the infection, clinical examinations aiming to evaluate rectal temperature, parasitemia and hematocrit were performed...
February 20, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29453596/effectiveness-of-the-sesquiterpene-%C3%AE-bisabolol-in-dogs-with-naturally-acquired-canine-leishmaniosis-an-exploratory-clinical-trial
#19
V Corpas-López, G Merino-Espinosa, C Acedo-Sánchez, V Díaz-Sáez, M C Navarro-Moll, F Morillas-Márquez, J Martín-Sánchez
The use of natural products is a promising approach for treating visceral leishmaniosis. (-)-α-Bisabolol is a sesquiterpene that have been proved active in vivo on Leishmania infantum-infected mice without showing toxicity. A single-centre, parallel-group, randomized, exploratory study was designed to assess its efficacy in a canine leishmaniosis model involving naturally infected dogs. In this clinical trial, 12 dogs were allocated into two groups and were treated with either meglumine antimoniate (100 mg/kg) through subcutaneous route or (-)-α-bisabolol (30 mg/kg) through oral route for two treatment series of 30 days, separated by a 30-day interval...
February 16, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29446002/genomic-characterization-of-the-first-oral-avian-papillomavirus-in-a-colony-of-breeding-canaries-serinus-canaria
#20
Daniel A Truchado, Michaël A J Moens, Sergio Callejas, Javier Pérez-Tris, Laura Benítez
Papillomaviruses are non-enveloped, DNA viruses that infect skin and mucosa of a wide variety of vertebrates, causing neoplasias or simply persisting asymptomatically. Avian papillomaviruses, with six fully sequenced genomes, are the second most studied group after mammalian papillomaviruses. In this study, we describe the first oral avian papillomavirus, detected in the tongue of a dead Yorkshire canary (Serinus canaria) and in oral swabs of the same bird and other two live canaries from an aviary in Madrid, Spain...
February 14, 2018: Veterinary Research Communications
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