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Veterinary Research Communications

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https://www.readbyqxmd.com/read/28429153/relative-abundance-of-heat-shock-proteins-and-clusterin-transcripts-in-spermatozoa-collected-form-boar-routinely-utilised-in-an-artificial-insemination-centre-preliminary-results
#1
A Zannoni, C Bernardini, A Zaniboni, E Ferlizza, D Ventrella, M L Bacci, M Forni
It is widely accepted that mature sperm contains RNA. The first hypothesis was that sperm RNAs have no functions of their own but are simply residues of spermatogenesis reflecting the events that occurred during their formation in the testes. More recently new discoveries have essentially expanded these views, showing that sperm mRNAs constitute a population of stable full-length transcripts, many of which are selectively retained during spermatogenesis and delivered to oocytes contributing to early embryo development...
April 21, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28405866/ethnoveterinary-health-management-practices-using-medicinal-plants-in-south-asia-a-review
#2
Shanoo Suroowan, Faisal Javeed, Mushtaq Ahmad, Muhammad Zafar, Mehwish Jamil Noor, Sadaf Kayani, Ali Javed, Mohamad Fawzi Mahomoodally
Animal rearing is the major occupation of most population of South Asian countries. Due to lack of resources and limited approach to modern medicine, most of the livestock raisers prefer to use plant-based traditional medicine also referred to as ethnoveterinary medicine (EVM). Indeed, the use of medicinal plants in South Asia dates back to several centuries with documented evidences. However, there is currently a dearth of documentation and compilation of use of medicinal plants for animal diseases in this part of the world...
April 12, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28389861/in-vitro-larvicidal-effect-of-a-hydroalcoholic-extract-from-acacia-cochliacantha-leaf-against-ruminant-parasitic-nematodes
#3
Agustín Olmedo-Juárez, Rolando Rojo-Rubio, Alejandro Zamilpa, Pedro Mendoza de Gives, Javier Arece-García, María Eugenia López-Arellano, Elke von Son-de Fernex
The aim of this study was to evaluate the in vitro lethal effect of a hydroalcoholic extract (HAE) from Acacia cochliacantha leaf against three gastrointestinal nematodes species (Haemonchus contortus, H. placei and Cooperia punctata) of domestic ruminants. The HAE was assessed using five concentrations: 100, 125, 175, 150 and 200 mg/ml; 0.5% Ivermectin was used as a positive control and distilled water, as negative control. The data were normalized using the square root and analysed with a completely randomized design through ANOVA analysis using the general lineal model (GLM) of the SAS program...
April 8, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28343363/sample-pooling-for-real-time-pcr-detection-and-virulence-determination-of-the-footrot-pathogen-dichelobacter-nodosus
#4
Sara Frosth, Ulrika König, Ann-Kristin Nyman, Anna Aspán
Dichelobacter nodosus is the principal cause of ovine footrot and strain virulence is an important factor in disease severity. Therefore, detection and virulence determination of D. nodosus is important for proper diagnosis of the disease. Today this is possible by real-time PCR analysis. Analysis of large numbers of samples is costly and laborious; therefore, pooling of individual samples is common in surveillance programs. However, pooling can reduce the sensitivity of the method. The aim of this study was to develop a pooling method for real-time PCR analysis that would allow sensitive detection and simultaneous virulence determination of D...
March 25, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28337578/evolution-of-the-corpus-luteum-volume-determined-ultrasonographically-and-its-relation-to-the-plasma-progesterone-concentration-after-artificial-insemination-in-pregnant-and-non-pregnant-dairy-cows
#5
C Gómez-Seco, B Alegre, F Martínez-Pastor, J G Prieto, J R González-Montaña, M E Alonso, J C Domínguez
The aim of this study was to assess the relationship of the evolution of the corpus luteum (CL) volume that was determined ultrasonographically with the pregnancy status in lactating dairy cows during early pregnancy. Ultrasound examinations were carried out on 76 cows following artificial insemination (AI). Plasma concentrations of progesterone were determined from blood samples collected at each ultrasound examination. Conception was confirmed by ultrasonography on day 30 after AI. Around day 14 post-insemination (p...
March 23, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28332027/erratum-to-identification-of-some-main-streptococcus-iniae-associated-proteins-relationship
#6
Fatima El Aamri, José Ángel Guillén, Daniel Padilla, Jimena Bravo, Belinda Vega, Félix Acosta, Fernando Real
No abstract text is available yet for this article.
March 22, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28281038/effect-of-intramammary-infusion-of-recombinant-bovine-gm-csf-and-il-8-on-cmt-score-somatic-cell-count-and-milk-mononuclear-cell-populations-in-holstein-cows-with-staphylococcus-aureus-subclinical-mastitis
#7
Yoshio Kiku, Tomomi Ozawa, Hideyuki Takahashi, Shiro Kushibiki, Shigeki Inumaru, Hiroyuki Shingu, Yuya Nagasawa, Atsushi Watanabe, Eiji Hata, Tomohito Hayashi
The effect of intramammary infusion of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) and interleukin-8 (rbIL-8) on mononuclear cell populations in quarters, somatic cell count (SCC) and the California Mastitis Test (CMT) score were investigated. From the selected cows with naturally occurring Staphylococcus aureus subclinical mastitis, one quarter of each cow were selected for the infusions of rbGM-CSF (400 μg/5 mL/quarter, n = 9), rbIL-8 (1 mg/5 mL/quarter, n = 9), and phosphate-buffered saline (5 mL/quarter, n = 7)...
March 9, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28210926/a-fatal-suppurative-pneumonia-in-piglets-caused-by-a-pathogenic-coagulase-positive-strain-of-staphylococcus-hyicus
#8
Mingyue Wang, Junying Hu, Lisai Zhu, Chunguang Guo, Haibing Lu, Changming Guo, Xin Li, Xinping Wang
Staphylococcus hyicus is one of the opportunistic pathogens that cause infections to animals. Early studies have demonstrated that S. hyicus is the causative agents of exudative epidermitis in pigs, arthritis in horses and chicken, mastitis in cow, and bacteremia, sepsis and multiple organ failure in humans. Here, we report the isolation and identification of a representative S. hyicus isolate, named JLHN15, from a pig farm with a disease characterized by bacteremia, suppurative pneumonia and fibrinous pericarditis...
February 16, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28194548/investigation-of-intra-herd-spread-of-mycobacterium-caprae-in-cattle-by-generation-and-use-of-a-whole-genome-sequence
#9
S Broeckl, S Krebs, A Varadharajan, R K Straubinger, H Blum, M Buettner
Single nucleotide polymorphisms (SNPs) calculated from whole genome sequencing (WGS) are ideally suited to study evolutionary relationships of pathogens and their epidemiology. Mycobacterium caprae infections have been documented frequently in cattle and red deer along the Bavarian and Austrian Alps during the last decade. However, little is still known about the transmission within cattle holdings and possible alterations of the genomes of M. caprae during such events. The aim of this study was to study the molecular epidemiology of bovine tuberculosis (bTB) in selected herds based on isolate-specific genome-wide SNPs and to perform a phylogenetic network analysis...
February 13, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28190219/temporal-changes-of-microrna-gga-let-7b-and-gga-let-7i-expression-in-chickens-challenged-with-subgroup-j-avian-leukosis-virus
#10
Jun Ji, Huiqin Shang, Huanmin Zhang, Hongxin Li, Jingyun Ma, Yingzuo Bi, Qingmei Xie
Two important microRNAs, gga-let-7b and gga-let-7i were examined for the relative expression in liver and bone marrow tissues from specific pathogen free chickens that were challenged either with GD1109 or NX0101 strain of subgroup J avian leukosis virus (ALV-J). The GD1109 strain of ALV-J reportedly causes hemangioma (HE) and NX0101 reportedly causes myeloma (ML) in susceptible chickens. Temporal changes of both gga-let-7b and gga-let-7i expression in ALV-J infected chickens were observed in contrast to its counterpart of a non-infected negative control group of chickens (P < 0...
February 11, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28161746/correlation-between-osteoarthritic-changes-in-the-stifle-joint-in-dogs-and-the-results-of-orthopedic-radiographic-ultrasonographic-and-arthroscopic-examinations
#11
Gabriel Ignacio Ramírez-Flores, Javier Del Angel-Caraza, Israel Alejandro Quijano-Hernández, Don A Hulse, Brian S Beale, José Mauro Victoria-Mora
Osteoarthritis (OA) is a chronic, degenerative disease affecting the articular cartilage and subchondral bone that causes pain and inhibits movement. The stifle's joint fibrous capsule contains the synovial membrane, which produces cartilage nutrients. A ruptured cranial cruciate ligament injures the joint and produces OA. Osteoarthritis diagnosis starts with clinical radiographic and ultrasonographic tests, although the latter is not used very much in dog and cat clinics for this purpose. The objective of this study was to establish the correlation among the results of orthopedic, radiographic, ultrasonographic examinations and structural anatomical changes revealed by arthroscopic evaluation to diagnose stifle joint OA and determine risk factors in the dogs affected...
February 4, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28160198/effects-of-neonatal-diarrhea-and-other-conditions-on-subsequent-productive-and-reproductive-performance-of-heifer-calves
#12
Fatemeh Aghakeshmiri, Mohammad Azizzadeh, Nima Farzaneh, Morteza Gorjidooz
Diarrhea is one of the most frequent diseases of neonatal calves in dairy herds. The aim of this study was to evaluate the effects of neonatal diarrhea and other conditions on subsequent first lactation milk production and reproductive performance of heifer calves up to the first calving. Seven hundred heifer calves (350 with and 350 without the history of diarrhea in first month of life) were monitored from birth until a year after calving. For each heifer, birth season, birth weight, ease of birth and occurrence of diseases from birth to the end of first lactation were recorded as independent variables...
February 3, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28144765/control-of-haemonchus-contortus-in-sheep-using-basidiocarps-of-agaricus-blazei-murril
#13
Thallyta Maria Vieira, Leydiana Duarte Fonseca, Gabriela Almeida Bastos, Viviane de Oliveira Vasconcelos, Maria Luíza França Silva, Franciellen Morais-Costa, Adriano Vinícius de Paiva Ferreira, Neide Judith Faria de Oliveira, Eduardo Robson Duarte
OBJECTIVE: This study evaluated the effects in vitro and in vivo of Agaricus blazei against Haemonchus contortus in sheep. METHODS: The in vitro efficacy of aqueous extract on egg hatching inhibition (EHI) was investigated and after 72 h incubation with varying concentrations the effects on, blastomeres, embryonated eggs, and first stage larvae (L1) were evaluated. Larval development inhibition (LDI) for dry powder and the aqueous extract were evaluated in fecal cultures of sheep infected with H...
January 31, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28012117/complete-genome-sequence-analysis-of-chicken-astrovirus-isolate-from-india
#14
Amrutlal K Patel, Ramesh J Pandit, Jalpa R Thakkar, Ankit T Hinsu, Vinod C Pandey, Joy K Pal, Kantilal S Prajapati, Subhash J Jakhesara, Chaitanya G Joshi
OBJECTIVE: Chicken astroviruses have been known to cause severe disease in chickens leading to increased mortality and "white chicks" condition. Here we aim to characterize the causative agent of visceral gout suspected for astrovirus infection in broiler breeder chickens. METHODS: Total RNA isolated from allantoic fluid of SPF embryo passaged with infected chicken sample was sequenced by whole genome shotgun sequencing using ion-torrent PGM platform. The sequence was analysed for the presence of coding and non-coding features, its similarity with reported isolates and epitope analysis of capsid structural protein...
March 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/27975177/involvement-of-histaminergic-and-noradrenergic-receptors-in-the-oxytocin-induced-food-intake-in-neonatal-meat-type-chicks
#15
Seyed Vahid Mirnaghizadeh, Morteza Zendehdel, Vahab Babapour
Oxytocin neurons have a physiological role in food intake and energy balance. Several studies have shown that central histaminergic and adrenergic systems synapse on oxytocin neurons but there is no information for their interaction on food intake regulation in birds. The purpose of this study was to examine the effects of intracerebroventricular (ICV) injection of α-fluoromethylhistidine (α-FMH, histidine decarboxylase inhibitor), chlorpheniramine (histamine H1 receptors antagonist), famotidine (histamine H2 receptors antagonist), thioperamide (histamine H3 receptors antagonist), prazosin (α1 receptor antagonist), yohimbine (α2 receptor antagonist), metoprolol (β1 adrenergic receptor antagonist), ICI 118,551 (β2 adrenergic receptor antagonist) and SR59230R (β3 adrenergic receptor antagonist) on oxytocin-induced hypophagia in 3-h food-deprived (FD3) neonatal broiler chicken...
March 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/27943152/oocyte-maturation-embryo-development-and-gene-expression-following-two-different-methods-of-bovine-cumulus-oocyte-complexes-vitrification
#16
Mehdi Azari, Mojtaba Kafi, Bita Ebrahimi, Roya Fatehi, Mahboobeh Jamalzadeh
OBJECTIVE: To examine the maturational competence, embryo development and expression of genes involved in oocyte maturation and cumulus expansion (GDF9, BMP15, HAS2, TNFAIP6, FGF17 and FSHr) following two standard methods of bovine COCs vitrification. METHODS: Bovine cumulus-oocyte complexes (COCs) were aspirated from slaughtered ovaries and then distributed into three groups: non-vitrified COCs (control), vitrification 1 group (V1); vitrification was performed by 15% ethylene glycol (EG) and 15% DMSO in holding media (TCM-199 with 20% FCS); and vitrification 2 group (V2); vitrification was performed by 40% EG in holding media...
March 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/27943151/l-carnitine-significantly-decreased-aging-of-rat-adipose-tissue-derived-mesenchymal-stem-cells
#17
Halimeh Mobarak, Ezzatollah Fathi, Raheleh Farahzadi, Nosratollah Zarghami, Sara Javanmardi
Mesenchymal stem cells are undifferentiated cells that have the ability to divide continuously and tissue regeneration potential during the transplantation. Aging and loss of cell survival, is one of the main problems in cell therapy. Since the production of free radicals in the aging process is effective, the use of antioxidant compounds can help in scavenging free radicals and prevent the aging of cells. The aim of this study is evaluate the effects of L-carnitine (LC) on proliferation and aging of rat adipose tissue-derived mesenchymal stem cells (rADSC)...
March 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/27933442/arterial-pulse-wave-propagation-velocity-in-healthy-dogs-by-pulse-wave-doppler-ultrasound
#18
Rodrigo B Nogueira, Lucas A Pereira, Alice F Basso, Ingrid S da Fonseca, Lorena A Alves
The aim of this study was to prospectively evaluate the carotid-femoral pulse wave velocity (PWV) values in healthy dogs using pulse wave Doppler ultrasound. A secondary aim was to determine the feasibility of this method and to report the intra- and interobserver reproducibilities of the PWV in conscious dogs. The data were studied in 30 healthy, adult, male (n = 15) and female (n = 15) dogs. The time interval marked between the R wave peak of the electrocardiogram and the intersection of the blood flow wave upstroke of the Doppler spectrum with the baseline of zero frequency was determined for the carotid (T1) and for the femoral (T2) arteries...
March 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/27896671/a-comparison-of-the-brainstem-auditory-evoked-response-in-healthy-ears-of-unilaterally-deaf-dogs-and-bilaterally-hearing-dogs
#19
M Plonek, J Nicpoń, K Kubiak, M Wrzosek
AIMS: Auditory plasticity in response to unilateral deafness has been reported in various animal species. Subcortical changes occurring in unilaterally deaf young dogs using the brainstem auditory evoked response have not been evaluated yet. The aim of this study was to assess the brainstem auditory evoked response findings in dogs with unilateral hearing loss, and compare them with recordings obtained from healthy dogs. METHODS: Brainstem auditory evoked responses (amplitudes and latencies of waves I, II, III, V, the V/I wave amplitude ratio, wave I-V, I-III and III-V interpeak intervals) were studied retrospectively in forty-six privately owned dogs, which were either unilaterally deaf or had bilateral hearing...
March 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/27896670/the-activation-of-the-ifn%C3%AE-induction-signaling-pathway-in-porcine-alveolar-macrophages-by-porcine-reproductive-and-respiratory-syndrome-virus-is-variable
#20
Christopher C Overend, Junru Cui, Marvin J Grubman, Antonio E Garmendia
BACKGROUND: It has been recognized that the expression of type I interferon (IFNα/β) may be suppressed during infection with porcine reproductive, respiratory syndrome virus (PRRSV). This causes profound negative effects on both the innate and adaptive immunity of the host resulting in persistence of infection. OBJECTIVE: Test the effects of PRRSV infection of porcine alveolar macrophages (PAMs), the main target cell, on the expression of interferon beta (IFNβ) and downstream signaling events...
March 2017: Veterinary Research Communications
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