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Wei-Hong Jian, Huan-Chih Wang, Chen-Hsiang Kuan, Ming-Hong Chen, Hsi-Chin Wu, Jui-Sheng Sun, Tzu-Wei Wang
The poor regenerative capability of stem cell transplantation in the central nervous system limits their therapeutic efficacy in brain injuries. The sustained inflammatory response, lack of structural support, and trophic factors deficiency restrain the integration and long-term survival of stem cells. Instead of exogenous stem cell therapy, here we described the synthesis of nanohybrid hydrogel containing sulfated glycosaminoglycan-based polyelectrolyte complex nanoparticles (PCN) to mimic the brain extracellular matrix and control the delivery of stromal-derived factor-1α (SDF-1α) and basic fibroblast factor (bFGF) in response to matrix metalloproteinase (MMP) for recruiting endogenous neural stem cells (NSC) and regulating their cellular fate...
May 8, 2018: Biomaterials
Zhengjie Lin, Jun Wu, Wei Qiao, Ying Zhao, Karen H M Wong, Paul K Chu, Liming Bian, Shuilin Wu, Yufeng Zheng, Kenneth M C Cheung, Frankie Leung, Kelvin W K Yeung
A range of magnesium ions (Mg2+ ) used has demonstrated osteogenic tendency in vitro. Hence, we propose to actualize this concept by designing a new system to precisely control the Mg2+ delivery at a particular concentration in vivo in order to effectively stimulate in-situ bone regeneration. To achieve this objective, a monodisperse core-shell microsphere delivery system comprising of poly (lactic-co-glycolic acid) (PLGA) biopolymer, alginate hydrogel, and magnesium oxide nano-particles has been designed by using customized microfluidic capillary device...
May 8, 2018: Biomaterials
Laura J Macdougall, Katherine L Wiley, April M Kloxin, Andrew P Dove
Controlled, three-dimensional (3D) cell culture systems are of growing interest for both tissue regeneration and disease, including cancer, enabling hypothesis testing about the effects of microenvironment cues on a variety of cellular processes, including aspects of disease progression. In this work, we encapsulate and culture in three dimensions different cancer cell lines in a synthetic extracellular matrix (ECM), using mild and efficient chemistry. Specifically, harnessing the nucleophilic addition of thiols to activated alkynes, we have created hydrogel-based materials with multifunctional poly(ethylene glycol) (PEG) and select biomimetic peptides...
May 7, 2018: Biomaterials
Siyoung Choi, Jens Friedrichs, Young Hye Song, Carsten Werner, Lara A Estroff, Claudia Fischbach
Bone metastasis is a leading cause of death in patients with breast cancer, but the underlying mechanisms are poorly understood. While much work focuses on the molecular and cellular events that drive breast cancer bone metastasis, it is mostly unclear what role bone extracellular matrix (ECM) properties play in this process. Bone ECM primarily consists of mineralized collagen fibrils, which are composed of non-stoichiometric carbonated apatite (HA) and collagen type I. Reduced bone mineral content is epidemiologically linked with increased risk of bone metastasis...
May 7, 2018: Biomaterials
Chelsea E T Stowell, Yadong Wang
Traditional tissue-engineered vascular grafts have yet to gain wide clinical use. The difficulty of scaling production of these cell- or biologic-based products has hindered commercialization. In situ tissue engineering bypasses such logistical challenges by using acellular resorbable scaffolds. Upon implant, the scaffolds become remodeled by host cells. This review describes the scientific and translational advantages of acellular, synthetic vascular grafts. It surveys in vivo results obtained with acellular synthetics over their fifty years of technological development...
May 5, 2018: Biomaterials
Chuying Ma, Ethan Gerhard, Di Lu, Jian Yang
Leveraging the multifunctional nature of citrate in chemistry and inspired by its important role in biological tissues, a class of highly versatile and functional citrate-based materials (CBBs) has been developed via facile and cost-effective polycondensation. CBBs exhibiting tunable mechanical properties and degradation rates, together with excellent biocompatibility and processability, have been successfully applied in vitro and in vivo for applications ranging from soft to hard tissue regeneration, as well as for nanomedicine designs...
May 4, 2018: Biomaterials
Parasmani Pageni, Peng Yang, Marpe Bam, Tianyu Zhu, Yung Pin Chen, Alan W Decho, Mitzi Nagarkatti, Chuanbing Tang
Over-prescription and improper use of antibiotics has led to the emergence of bacterial resistance, posing a major threat to public health. There has been significant interest in the development of alternative therapies and agents to combat antibiotic resistance. We report the preparation of recyclable magnetic iron oxide nanoparticles grafted with charged cobaltocenium-containing metallopolymers by surface-initiated reversible addition-fragmentation chain transfer (RAFT) polymerization. β-Lactam antibiotics were then conjugated with metallopolymers to enhance their vitality against both Gram-positive and Gram-negative bacteria...
May 3, 2018: Biomaterials
Ranran Zhao, Xiaolong Liang, Bo Zhao, Min Chen, Renfa Liu, Sujuan Sun, Xiuli Yue, Shumin Wang
To improve the non-invasive therapeutic efficacy for ER positive breast cancer (ER+ BC), we fabricated a multifunctional FOXA1 loaded porphyrin microbubble to combine photodynamic therapy (PDT) and gene therapy of FOXA1 knockdown (KD) with ultrasound targeted microbubble destruction (UTMD) technology under the guidance of contrast enhanced ultrasound (CEUS). Cationic porphyrin microbubbles (CpMBs) were firstly fabricated from a porphyrin grafted lipid with two cationic amino groups (PGL-NH2) and fluorocarbon inert gas of C3 F8 ...
May 3, 2018: Biomaterials
Ang Li, Yiyong Wei, Clark Hung, Gordana Vunjak-Novakovic
Cartilage extracellular matrix (ECM) has been used for promoting tissue engineering. However, the exact effects of ECM on chondrogenesis and the acting mechanisms are not well understood. In this study, we investigated the chondrogenic effects of cartilage ECM on human mesenchymal stem cells (MSCs) and identified the contributing molecular components. To this end, a preparation of articular cartilage ECM was supplemented to pellets of chondrogenically differentiating MSCs, pellets of human chondrocytes, and bovine articular cartilage explants to evaluate the effects on cell proliferation and the production of cartilaginous matrix...
May 3, 2018: Biomaterials
Su-Hwan Kim, Sang-Hyuk Lee, Ju-Eun Lee, Sung Jun Park, Kyungmin Kim, In Seon Kim, Yoon-Sik Lee, Nathaniel S Hwang, Byung-Gee Kim
We report on a tissue adhesive hydrogel based on novel recombinant tyrosinase mediated crosslinking. The adhesive hydrogels were fabricated by the site-directed coupling of tyramine-conjugated hyaluronic acid (HA_t, 1% w/v) and gelatin (3% w/v) (HG_gel) with novel tyrosinase derived from Streptomyces avermitilis (SA_Ty). The enzyme-based crosslinking by SA_Ty was fast, with less than 50 s for complete gelation, and the SA_Ty based crosslinking enhanced the physical properties and adhesive strength of the hydrogel significantly with the native tissue samples...
May 3, 2018: Biomaterials
Yingchao Liu, Jie Tan, Yi Zhang, Jianfeng Zhuang, Mingxu Ge, Ben Shi, Jiao Li, Ge Xu, Shangchen Xu, Chunhai Fan, Chunchang Zhao
Distinguishing tumor from adjacent non-cancerous tissue can be problematic during surgical treatment of malignant glioma. Consequently, a novel approach to selective discrimination is required. The goal of this study was to determine whether a fluorescent probe activated by γ-Glutamyltranspeptidase (GGT), an enzyme that is overexpressed on glioma cell membranes but only minimally expressed in normal brain tissue, could be used to visualize glioma margins. Here, we showed that the GGT-activatable fluorescent probe (NC-B-Cys-γ-Glu) provided real-time in situ tracking of enzyme activity that accurately distinguished glioma from healthy brain tissue...
April 27, 2018: Biomaterials
Sadegh Davoudi, Chih-Ying Chin, Michael J Cooke, Roger Y Tam, Molly S Shoichet, Penney M Gilbert
Adult skeletal muscle tissue harbors the capacity for self-repair due to the presence of tissue resident muscle stem cells (MuSCs). Advances in the area of prospective MuSC isolation demonstrated the potential of cell transplantation therapy as a regenerative medicine strategy to restore strength and long-term regenerative capacity to aged, injured, or diseased skeletal muscle tissue. However, cell loss during ejection, limits to post-injection proliferation, and poor donor cell dispersion distal to the injection site are amongst hurdles to overcome to maximize MuSC transplant impact...
April 26, 2018: Biomaterials
Yang Liu, Zhi-Ting Cao, Cong-Fei Xu, Zi-Dong Lu, Ying-Li Luo, Jun Wang
Inflammation is closely related to the development of many diseases and is commonly characterized by abnormal infiltration of immune cells, especially neutrophils. The current therapeutics of inflammatory diseases give little attention to direct modulation of these diseases with respect to immune cells. Nanoparticles are applied for efficient drug delivery into the disease-related immune cells, but their performance is significantly affected by their surface properties. In this study, to optimize the properties of nanoparticles for modulating neutrophils-related inflammation, we prepared a library of poly(ethylene glycol)-b-poly(lactide-co-glycolide) (PEG-b-PLGA)-based cationic lipid-assisted nanoparticles (CLANs) with different surface PEG density and surface charge...
April 26, 2018: Biomaterials
Jintong Liu, Ping Du, Hui Mao, Lei Zhang, Huangxian Ju, Jianping Lei
Nonspecific distribution of photosensitizer and the intrinsic hypoxic condition in the tumor microenvironment are two key factors limiting the efficacy of O2 -dependent photodynamic therapy (PDT). Herein, a dual-triggered oxygen self-supported nanosystem using black phosphorus nanosheet (BPNS) as both photosensitizer and nanocarrier was developed to enhance PDT for tumors within hypoxic microenvironment. The BPNS platform was functionalized with folate and a blocker DNA duplex of 5'-Cy5-aptamer-heme/3'-heme labeled oligonucleotides...
April 26, 2018: Biomaterials
Di Zhang, Feng Feng, Qilong Li, Xiuyu Wang, Li Yao
Nanomaterials-based photodynamic therapy (PDT) has been used to treat malignant cells. However, the intrinsic impact of nanomaterials-based PDT on mechanical properties of intractable tumor cells is not well understood. Herein, we demonstrated that the mechanical forces of Taxol-resistant tumor cells were decreased by nanopurpurin-based PDT destructing extracellular matrix (ECM), increasing therapy sensitivity and repressing tumor metastasis. Combining FIRMS and general confocal microscope, we observed that the disruption of ECM by photodynamic reaction of P18-nanoconfined liposome (P18⊂L) induced a decrease of adhesion force and biomechanical properties of Taxol-resistant cells through the attenuation of actomyosin-based contractility thereby inhibiting cell migration and metastasis in vivo...
April 25, 2018: Biomaterials
Tomoki Takashina, Takayoshi Koyama, Satoshi Nohara, Masakatsu Hasegawa, Akira Ishiguro, Kenta Iijima, Jun Lu, Mari Shimura, Tadashi Okamura, Tetsushi Sakuma, Takashi Yamamoto, Yukihito Ishizaka
Cellular reprogramming is a promising technology in regenerative medicine, but most studies have been performed by using expression vectors. For future clinical applications, it is necessary to establish a system in which cell engineering can be manipulated without any risk of damaging the genome. Here, we identified a cell-penetrating peptide composed of 10 amino acids (RIFIHFRIGC) with nuclear trafficking activity and found that it was significantly more potent than a Tat-derived peptide or polyarginine peptide (R11)...
April 25, 2018: Biomaterials
Xinyu Liu, Jiawei Sun, Weiping Gao
Protein modification with polymers has led to intriguing and new types of bioconjugates. They combine the tunable physicochemical properties of the polymers with the specific biological activity of the proteins. These unique attributes of protein-polymer conjugates render them interesting and useful in biomedicine. However, the application potential of protein-polymer conjugates is limited by the mostly non-selective protein modification with polymers due to the lack of site-selective protein modification technology...
April 25, 2018: Biomaterials
Jessica D Weaver, Devon M Headen, Michael D Hunckler, Maria M Coronel, Cherie L Stabler, Andrés J García
The use of immunoisolating macrodevices in islet transplantation confers the benefit of safety and translatability by containing transplanted cells within a single retrievable device. To date, there has been limited development and characterization of synthetic poly(ethylene glycol) (PEG)-based hydrogel macrodevices for islet encapsulation and transplantation. Herein, we describe a two-component synthetic PEG hydrogel macrodevice system, designed for islet delivery to an extrahepatic islet transplant site, consisting of a hydrogel core cross-linked with a non-degradable PEG dithiol and a vasculogenic outer layer cross-linked with a proteolytically sensitive peptide to promote degradation and enhance localized vascularization...
April 25, 2018: Biomaterials
Sarah E Lightfoot Vidal, Kasey A Tamamoto, Hanh Nguyen, Rosalyn D Abbott, Dana M Cairns, David L Kaplan
Current commercially available human skin equivalents (HSEs) are used for relatively short term studies (∼1 week) due in part to the time-dependent contraction of the collagen gel-based matrix and the limited cell types and skin tissue components utilized. In contrast, here we describe a new matrix consisting of a silk-collagen composite system that provides long term, stable cultivation with reduced contraction and degradation over time. This matrix supports full thickness skin equivalents which include nerves...
April 24, 2018: Biomaterials
Haijiao Liu, Luke A MacQueen, Jenna F Usprech, Hoda Maleki, Krista L Sider, Matthew G Doyle, Yu Sun, Craig A Simmons
Native and engineered tissue development are regulated by the integrative effects of multiple microenvironmental stimuli. Microfabricated bioreactor array platforms can efficiently dissect cue-response networks, and have recently integrated critical 2D and 3D mechanical stimulation for greater physiological relevance. However, a limitation of these approaches is that assessment of tissue functional properties is typically limited to end-point analyses. Here we report a new deformable membrane platform with integrated strain sensors that enables mechanical stretching or compression of 3D cell-hydrogel arrays and simultaneous measurement of hydrogel construct stiffness in situ...
April 23, 2018: Biomaterials
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