journal
MENU ▼
Read by QxMD icon Read
search

Human Immunology

journal
https://www.readbyqxmd.com/read/28899793/comprehensive-assessment-for-serum-treatment-for-single-antigen-test-for-detection-of-hla-antibodies
#1
Xiaohai Zhang, Nancy L Reinsmoen
The single antigen test is widely used in the field of transplantation to determine the specificity of HLA antibodies. It will be beneficial to standardize the procedure of the single antigen test among HLA laboratories. It is not uncommon that single antigen testing on native sera fails to detect antibodies with very high concentrations. It has been shown that cleavage products of activated complement components may mask strongly binding antibodies in single antigen testing. To overcome inhibition by the activated complement products, sera are pretreated with ethylenediaminetetraacetic acid (EDTA), dithiothreitol (DTT), or heat inactivation before single antigen testing...
September 9, 2017: Human Immunology
https://www.readbyqxmd.com/read/28887054/hla-dpb1-matching-in-unrelated-hematopoietic-stem-cell-transplantation-program-contributes-to-a-higher-incidence-of-disease-relapse
#2
Marija Burek Kamenaric, Marija Maskalan, Zorana Grubic, Mirta Mikulić, Ranka Serventi Seiwerth, Nadira Duraković, Radovan Vrhovac, Katarina Stingl Jankovic, Renata Zunec
The impact of patient/donor matching for HLA-A, -B, -C, -DRB1 and -DQB1 genes in hematopoietic stem cell transplantation (HSCT) is well-recognized, but typing for additional genes, such as HLA-DPB1, is still controversial. Based on defined T-cell epitope (TCE) groups, all HLA-DPB1 mismatches can be classified as permissive or non-permissive. In this retrospective study we analysed 82 patient/matched unrelated donor (MUD) pairs who underwent HSCT, and explored the impact of HLA-DPB1 matches, permissive and non-permissive mismatches on transplantation outcomes...
September 5, 2017: Human Immunology
https://www.readbyqxmd.com/read/28867178/associations-of-erap1-coding-variants-and-domain-specific-interaction-with-hla-c%C3%A2-06-in-the-early-onset-psoriasis-patients-of-india
#3
Anamika Das, Aditi Chandra, Joyeeta Chakraborty, Abhijit Chattopadhyay, Swapan Senapati, Gobinda Chatterjee, Raghunath Chatterjee
Interferon-γ-induced aminopeptidase ERAP1 trims peptides within the endoplasmic reticulum so that they can be loaded onto MHC class I and presented to the CD8(+) T-cells. ERAP1 association and its interaction with HLA-C∗06 is controversial across different populations. We have investigated the association and possible functional role of non-synonymous SNPs at different exons of ERAP1 (rs26653: Arg127Pro, rs30187: Lys528Arg and rs27044: Gln730Glu) and their interactions with HLA-C∗06 in psoriasis. Significant associations of HLA-C∗06 (OR=5...
September 1, 2017: Human Immunology
https://www.readbyqxmd.com/read/28865670/estimation-of-optimal-donor-number-in-bone-marrow-donor-registry-hong-kong-s-experience
#4
Janette Kwok, Mengbiao Guo, Wanling Yang, Patrick Ip, Godfrey C F Chan, Jenny Ho, C K Lee, Derek Middleton, Liwei Lu
Better outcome for hematopoietic stem cell transplantation (HSCT) requires optimal matching between donor and recipient at the HLA-A, -B, -C, and -DRB1 loci. This study estimates the likelihood of identifying HLA matched donors in Hong Kong. 7595 volunteer unrelated Chinese donors at the Hong Kong Bone Marrow Donor Registry were typed with HLA-A, -B, -C and -DRB1 genotypes. The matching probabilities for 8/8 and 7/8 HLA match via the matching models were determined. Based on current 100,000 donors in the HKBMDR, the matching probabilities are 45% at 8/8 HLA match and 65% at 7/8 match...
August 31, 2017: Human Immunology
https://www.readbyqxmd.com/read/28844894/hla-gene-and-haplotype-frequencies-in-a-nagaybaks-population-from-the-chelyabinsk-region-russian-south-urals
#5
T A Suslova, A L Burmistrova, M N Vavilov, M S Chernova, E B Khromova, S V Belyaeva, O N Zaripova, D S Stashkevich, A S Galkin, C Darke
A total of 112 Nagaybaks, a Turkic ethnoconfessional group living mainly in the Nagaybak district of the Chelyabinsk Region of Russian South Urals, were genotyped for HLA-A, -B, -DRB1, -DQA1 and -DQB1 loci using PCR-SSP (low-resolution) and HLA-A29 (high-resolution). All loci were in Hardy-Weinberg equilibrium (all p values >0.1 thus showing no locus-level deviations. The genotype data are available in the Allele Frequencies Net Database under the population name ''Russia, South Ural, Chelyabinsk Region, Nagaybaks" and the identifier (AFND0003397)...
August 24, 2017: Human Immunology
https://www.readbyqxmd.com/read/28813642/buccal-swab-genomic-dna-fragmentation-predicts-likelihood-of-successful-hla-genotyping-by-next-generation-sequencing
#6
Maureen C Montgomery, Rosanne Petraroia, Eric T Weimer
Many clinical human leukocyte antigen (HLA) laboratories are adopting next-generation sequencing (NGS) technology for HLA genotyping. There have been several reports of the cost-benefit and reduction in turn-around-time provided by NGS. Ninety-six percent of buccal swabs and peripheral blood samples had reportable HLA genotyping by NGS. The HLA loci most likely to fail genotyping from buccal swabs were DQB1, DPB1, and DPA1. Successful buccal swab samples had significantly less genomic DNA fragmentation compared to buccal swab samples that were unsuccessful...
August 13, 2017: Human Immunology
https://www.readbyqxmd.com/read/28803786/distribution-of-hla-a-b-and-drb1-antigenic-groups-and-haplotypes-from-the-brazilian-bone-marrow-donor-registry-redome
#7
Leandro Torres, Luis Fernando da Silva Bouzas, Alexandre Almada, Luis Cristovão Moraes de Sobrino Porto, Eliana Abdelhay
To improve assistance for patients awaiting a bone marrow transplant from an unrelated donor, it is important to genetically characterize the Brazilian volunteer bone marrow donors registry (REDOME). Our objective was to describe the antigenic groups and haplotype frequencies of HLA-A, HLA-B and HLA-DRB1 in the five regions of Brazil and by self-reported ethnicity groups using the REDOME data. Our study included 3,038,286 individuals. HLA antigenic groups and haplotype frequencies were estimated using an Expectation-Maximization (EM) algorithm...
August 10, 2017: Human Immunology
https://www.readbyqxmd.com/read/28803785/vascular-endothelial-growth-factor-vegf-gene-polymorphisms-rs699947-rs833061-and-rs2010963-and-psoriatic-risk-in-south-indian-tamils
#8
Anjana Sudhesan, Medha Rajappa, Laxmisha Chandrashekar, P H Ananthanarayanan, D M Thappa, Santhosh Satheesh, Adithan Chandrasekaran
BACKGROUND: Psoriasis is a chronic inflammatory disease of the skin. Vascular endothelial growth factor (VEGF), a pro-angiogenic factor, is involved in the pathogenesis of psoriasis. Being highly polymorphic, several SNPs of VEGF have been reported to be associated with increased risk of psoriasis. OBJECTIVES: We determined the association of VEGF gene polymorphisms with risk of psoriasis in South Indian Tamils. METHODS: 300 cases of psoriasis and 300 controls were recruited in this case-control study...
August 10, 2017: Human Immunology
https://www.readbyqxmd.com/read/28764923/association-of-rs1285933-single-nucleotide-polymorphism-in-clec5a-gene-with-dengue-severity-and-its-functional-effects
#9
Caroline Xavier-Carvalho, Renata Duarte da Silva Cezar, Naishe Matos Freire, Carla Mola, Victor Fiesta, Thiago Gomes de Toledo-Pinto, Luciana Gomes Fialho Torres, Rodrigo Feliciano do Carmo, Luydson Richardson Silva Vasconcelos, Marli Tenório Cordeiro, Paulo Baptista, Elzinandes Leal de Azeredo, Rivaldo Venâncio da Cunha, Luiz José de Souza, Antonio Guilherme Pacheco, Claire Fernandes Kubelka, Patrícia Moura, Milton Ozorio Moraes
Outbreaks of the Zika, dengue, and chikungunya viruses, especially in the Americas, pose a global threat due to their rapid spread and difficulty controlling the vector. Extreme phenotypes are often observed, from asymptomatic to severe clinical manifestations, which are well-studied in dengue. Host variations are also important contributors to disease outcomes, and many case-control studies have associated single nucleotide polymorphisms (SNPs) with severe dengue. Here, we found that the TC genotype and T-carriers for SNP rs1285933 in the C-type lectin superfamily member 5 (CLEC5A) gene was associated with severe dengue in a Northern Brazilian population (OR=2...
July 29, 2017: Human Immunology
https://www.readbyqxmd.com/read/28734803/distribution-of-kir-genes-and-kir2ds4-gene-variants-in-two-mexican-mestizo-populations
#10
Machado-Sulbaran Andrea Carolina, Muñoz-Valle José Francisco, Ramírez-Dueñas María Guadalupe, Baños-Hernández Christian Johana, Machuca-Graciano Omar, Velarde-De la Cruz Erandi Enif, Parra-Rojas Isela, Sánchez-Hernández Pedro Ernesto
Killer immunoglobulin-like receptors (KIR) are transmembrane proteins that regulate NK and T cell subsets by recognizing HLA-I molecules as ligands. The KIR gene family consists of 16 genes, located at chromosome 19q13.4. KIR gene frequencies vary among populations. In Mexico, HLA and genetic ancestry studies show that Mestizo populations have different genetic backgrounds based on admixture with European, African, and Asian ancestry. This study aimed to evaluate the frequencies of KIR genes and genotypes in Guerrero and Jalisco, two Mexican Mestizo populations located in the south and the west of the country, respectively, and to compare these frequencies with those of other populations...
July 19, 2017: Human Immunology
https://www.readbyqxmd.com/read/28732720/impact-on-mid-term-kidney-graft-outcomes-of-pretransplant-anti-hla-antibodies-detected-by-solid-phase-assays-do-donor-specific-antibodies-tell-the-whole-story
#11
Jorge Malheiro, Sandra Tafulo, Leonídio Dias, La Salete Martins, Isabel Fonseca, Idalina Beirão, António Castro-Henriques, António Cabrita
The detrimental impact of preformed anti-HLA donor-specific antibodies (DSA) is well defined, contrarily to non-donor-specific antibodies (NDSA). We sought to evaluate their clinical impact in a cohort of 724 kidney graft recipients in whom anti-HLA antibodies were thoroughly screened and identified in pre-transplant sera by solid-phase assays. NDSA or DSA were detected in 100 (13.8%) and 47 (6.5%) recipients respectively, while 577 (79.7%) were non-allosensitized (NaS). Incidence of antibody-mediated rejection at 1-year was 0...
July 19, 2017: Human Immunology
https://www.readbyqxmd.com/read/28732721/assessing-a-single-targeted-next-generation-sequencing-for-human-leukocyte-antigen-typing-protocol-for-interoperability-as-performed-by-users-with-variable-experience
#12
Manish J Gandhi, Deborah Ferriola, Curt Lind, Jamie L Duke, Anh Huynh, Anna Papazoglou, Kate Mackiewicz, Mette Christiansen, Wei Dong, Susan Hsu, Dawn Thomas, Brittany Schneider, Erin Pierce, Jane Kearns, Malek Kamoun, Dimitri Monos, Medhat Askar
BACKGROUND: A simplified protocol for HLA-typing -by NGS, developed for use with the Illumina MiSeq, was performed by technologists with varying NGS experience to assess accuracy and reproducibility. METHODS: Technologists from six laboratories typed the same 16 samples at HLA-A, B, C, DRB1, and DQB1. The protocol includes long range PCR, library preparation, and paired-end 250bp sequencing. Two indexing strategies were employed: locus-specific indexing whereby each locus was tagged uniquely and sample-specific indexing whereby all 5 loci for a sample were pooled prior to library preparation...
July 18, 2017: Human Immunology
https://www.readbyqxmd.com/read/28716698/cytokine-balance-and-behavioral-intervention-findings-from-the-peer-approaches-to-lupus-self-management-pals-project
#13
Edith M Williams, J Madison Hyer, Ramakrishnan Viswanathan, Trevor D Faith, Leonard Egede, Jim C Oates, Gailen D Marshall
The Peer Approaches to Lupus Self-Management program sought to address the disparate impact of systemic lupus erythematosus (SLE) on African American women through a peer mentoring intervention with aims of reducing stress, anxiety, and depression. Given the association between psychological health and immune function this study examines the relationship between patient reported outcomes (PROs) in these domains and immunologic indicators of disease activity. Twenty-three African American women with SLE served as mentees in the intervention from whom PRO measures were collected at the outset, midpoint, and end of the 12week pilot study...
July 14, 2017: Human Immunology
https://www.readbyqxmd.com/read/28716697/copy-number-variations-of-the-il-22-gene-are-associated-with-ankylosing-spondylitis-a-case-control-study-in-chinese-han-population
#14
Xu Zhang, Xiaona Li, Renfang Han, Mengya Chen, Yaping Yuan, Xingxing Hu, Mengmeng Wang, Rui Li, Xiao Yang, Qing Xia, Yubo Ma, Jiajia Yang, Jingjing Tong, Shengqian Xu, Jianhua Xu, Zongwen Shuai, Faming Pan
IL-22 provides a new insight into the mechanisms of autoimmunity, and copy number variations (CNVs) are associated with autoimmune diseases. This study aims to explore the association of IL-22 gene CNVs with ankylosing spondylitis (AS) susceptibility. The copy numbers of IL-22 gene (2 fragments: IL-22_1, IL-22_2) were examined by AccuCopy™ methods in a cohort of 649 AS patients and 628 controls. Association of IL-22 CNVs and AS susceptibility was analyzed, and AS risk was estimated by Odds Ratio (ORs) and 95% confidence intervals (CIs), and the Benjamini-Hochberg method was applied to regulate the false discovery rate (FDR)...
July 14, 2017: Human Immunology
https://www.readbyqxmd.com/read/28705752/polymorphisms-of-the-human-platelet-antigen-1-2-3-5-and-15-systems-and-acute-cellular-liver-transplant-rejection
#15
Hansjörg Thude, Wiebke Bischoff, Martina Sterneck, Matthias Marget, Björn Nashan, Martina Koch
The human platelet antigen (HPA)-1, -2, -3, -5, and -15 systems are characterized as polymorphic alloantigens expressed on platelets and endothelial cells. In this retrospective study, we investigated, whether HPA-1, -2, -3, -5, and -15 incompatibilities are associated with acute cellular liver transplant rejection. A total of 96 Caucasian liver transplant recipients and corresponding donors were analyzed, 43 with biopsy proven acute cellular rejection (BPAR) and 53 without acute cellular rejection (No-BPAR)...
July 10, 2017: Human Immunology
https://www.readbyqxmd.com/read/28694051/nomenclature-for-factors-of-the-hla-system-update-may-2017
#16
Steven G E Marsh
No abstract text is available yet for this article.
July 8, 2017: Human Immunology
https://www.readbyqxmd.com/read/28694050/nomenclature-for-factors-of-the-hla-system-update-june-2017
#17
Steven G E Marsh
No abstract text is available yet for this article.
July 8, 2017: Human Immunology
https://www.readbyqxmd.com/read/28694049/nomenclature-for-factors-of-the-hla-system-update-april-2017
#18
Steven G E Marsh
No abstract text is available yet for this article.
July 8, 2017: Human Immunology
https://www.readbyqxmd.com/read/28743386/corrigendum-to-calculated-panel-reactive-antibody-with-decimals-a-refined-metric-of-access-to-transplantation-for-highly-sensitized-candidates-hum-immunol-78-3-2017-252-256
#19
https://www.readbyqxmd.com/read/28743385/corrigendum-to-cd28-and-ptpn22-are-associated-with-susceptibility-to-rheumatoid-arthritis-in-egyptians-hum-immunol-77-2016-522-526
#20
Mohsen M Hegab, Aml Fawzy Abdelwahab, Janine Mia Rudolph, Ali M El-Sayed Yousef, Mohamed Nabil Salem, Walaa El-Baz, Sherry Abdelrhman, Fatemah Elshabacy, Abdelazim Alhefny, Wagida Abouraya, Saleh Mohamed Ibrahim, Gaafar Ragab
No abstract text is available yet for this article.
July 2017: Human Immunology
journal
journal
28052
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"