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Human Immunology

Aviva Geretz, Philip K Ehrenberg, Alain Bouckenooghe, Marcelo A Fernández Viña, Nelson L Michael, Danaya Chansinghakule, Kriengsak Limkittikul, Rasmi Thomas
The human leukocyte antigen (HLA) genes are highly variable and are known to play an important role in disease outcomes, including infectious diseases. Prior knowledge of HLA polymorphisms in a population usually forms the basis for an effective case-control study design. As a prelude to future disease association analyses, we report HLA class I and II diversity in 334 unrelated donors from a Dengue vaccine efficacy trial conducted in Thailand. Long-range PCR amplification of six HLA loci was performed on DNA extracted from saliva samples...
September 19, 2018: Human Immunology
Anita S Chong
A resurgent interest in the role of B cells following solid organ transplantation is being driven by clinical data suggesting that antibody mediated rejection (AMR) is a major cause of dysfunction and organ transplant failure. These observations suggest that, in a subset of patients, current immunotherapies are failing to control the development of alloantibody responses, or failing to reverse the production or the effects of alloantibodies. Quantification of donor-specific antibodies (DSA) has proven to be an imperfect predictor of AMR, and efforts to improve DSA quantification anticipate that this will result in improved predictive power...
September 18, 2018: Human Immunology
Yvonne R Thorstenson, Lisa E Creary, Huang Huang, Virginie Rozot, Tracy T Nguyen, Farbod Babrzadeh, Sandeep Kancharla, Marilyn Fukushima, Raquel Kuehn, Chunlin Wang, Ming Li, Sujatha Krishnakumar, Michael Mindrinos, Marcelo A Fernandez Viña, Thomas J Scriba, Mark M Davis
The development of next-generation sequencing (NGS) methods for HLA genotyping has already had an impact on the scope and precision of HLA research. In this study, allelic resolution HLA typing was obtained for 402 individuals from Cape Town, South Africa. The data were produced by high-throughput NGS sequencing as part of a study of T-cell responses to Mycobacterium tuberculosis in collaboration with the University of Cape Town and Stanford University. All samples were genotyped for 11 HLA loci, namely HLA-A, -B, -C, -DPA1, -DPB1, -DQA1, -DQB1, -DRB1, -DRB3, -DRB4, and -DRB5...
September 18, 2018: Human Immunology
Mengkai Shieh, Nilesh Chitnis, Peter Clark, Frederick Bradley Johnson, Malek Kamoun, Dimitri Monos
Cell surface expression of HLA-DP is allele specific. SNP rs9277534 (A/G), located in the 3'UTR of the DPB1 gene, has been associated with either low (A) or high (G) expression of DP on the cell surface. Considering the role of miRNAs in the regulation of gene expression, we computationally identified the miRNAs of two BLCLs, PGF and COX, predicted to interact with their corresponding DPB1 transcripts, DPB1∗04:01:01:01-low expression and DPB1∗03:01:01:01-high expression. The identified target sequences are located primarily in intron 2 and the 3'UTR...
September 15, 2018: Human Immunology
Panagiotis Mallis, Efstathios Michalopoulos, Amalia Dinou, Maria Spyropoulou Vlachou, Efrosyni Panagouli, Aggeliki Papapanagiotou, Eva Kassi, Catherine Stavropoulos Giokas
Worldwide, there is a great need of small diameter vascular grafts that can be used in human disorders such as cardiovascular and peripheral vascular disease. Until now, severe adverse reactions are caused from the use of synthetic or animal derived grafts, while the use of autologous vessels is restricted only in a small number of patients. The limited availability of the vessels might be resolved by the use of HLA-matched vascular grafts utilizing the decellularized human umbilical arteries. In this study, human umbilical arteries were decellularized and then were repopulated with Mesenchymal Stromal Cells...
September 10, 2018: Human Immunology
Mehrdad Hefazi, William J Hogan, Laurie L Wakefield, Manish J Gandhi
Despite advances in HLA matching, graft failure remains a serious complication after allogeneic hematopoietic cell transplantation (HCT). Pre-formed anti-HLA donor-specific antibodies (DSAs) have been associated with graft failure, but the impact of newly developing (de novo) anti-HLA antibodies on HCT outcomes remains unknown. Here, we present the first reported case of graft failure associated with de novo anti-HLA-DPB1 DSA after a 10/10 matched unrelated donor HCT. Based on this observation, testing for DSA should be considered in case of unexplained pancytopenia after allogeneic transplantation, especially after reduced intensity conditioning...
August 22, 2018: Human Immunology
Williams Walana, Jing-Jing Wang, Iddrisu Baba Yabasin, Michael Ntim, Sylvanus Kampo, Mahmoud Al-Azab, Abdalkhalig Elkhider, Eugene Dogkotenge Kuugbee, Jya-Wei Cheng, John R Gordon, Fang Li
IL-8 is elevated during inflammation, and it initiates cascade of down-stream reactions. Its antagonist, CXCL8 (3-72) K11R/G31P (G31P), represses inflammatory reactions via competitive binding to CXC chemokine family, preferentially G protein-couple receptors (GPCRs) CXCR1/2. This study reports the effect of G31P on the transcription profile of lipopolysaccharide (LPS) induced inflammation in THP-1 monocytes ex-vivo. LPS (1 µg/ml) induced elevation of IL-8 was significantly reduced by G31P (20 µg/ml and 30 µg/ml), with relatively increased inhibition of CXCR2 than CXCR1...
August 17, 2018: Human Immunology
Nicole M Valenzuela, Medhat Askar, Sebastiaan Heidt, Peter Jindra, Abeer Madbouly, David Pinelli, Annette Jackson, Luis G Hidalgo
Histocompatibility testing, and HLA antibody screening in particular, varies in practice among laboratories. Currently, standards are lacking regarding the reporting of testing methods in publications. It is essential that scientific methods are rigorously and transparently described upon publication, so that results can be accurately interpreted and independently corroborated. Additionally, this would allow work groups to compile diverse data to achieve clinically significant conclusions from meta-analyses...
August 17, 2018: Human Immunology
Navchetan Kaur, Evan P Kransdorf, Marcelo J Pando, Martin Maiers, Bryan Ray, Jar-How Lee, Peter Lalli, Cathi L Murphey, Robert A Bray, Loren Gragert
BACKGROUND: Histocompatibility labs must convert molecular HLA typing data to antigen equivalencies for entry into the United Network for Organ Sharing (UNOS) UNet system. While an Organ Procurement and Transplantation Network (OPTN) policy document provides general guidelines for conversion, the process is complex because no antigen mapping table is available. We present a UNOS antigen equivalency table for all IPD-IMGT/HLA alleles at the A, B, C, DRB1, DRB3/4/5, DQA1, and DQB1 loci...
August 15, 2018: Human Immunology
Daria Bortolotti, Filippo Rossignoli, Antonella Rotola, Diana Campioni, Rosario Cultrera, Giulia Grisendi, Massimo Dominici, Roberta Rizzo
OBJECTIVES: Mesenchymal stromal/stem cells have immunosuppressive functions. Our previous results demonstrated that one of the players of this immunomodulation can be ascribed to the Human Leukocyte Antigen-G. HLA-G, a non classical HLA class I antigen, is involved in immune tolerance during pregnancy, organ transplantation, autoimmune and inflammatory diseases. In this study we wanted to verify whether human endometrial decidual tissue derived (EDT)-MSC could express HLA-G. Additionally we assessed the permissivity to Human Herpesvirus infections, using HSV-1 as a model, and the possible effect on EDT-MSC immunosuppressive functions towards peripheral blood mononuclear cell (PBMC) proliferation...
August 14, 2018: Human Immunology
Christina E M Voorter, Ben Matern, Thuong Hien Tran, Annette Fink, Blanka Vidan-Jeras, Sendi Montanic, Gottfried Fischer, Ingrid Fae, Dianne de Santis, Rebecca Whidborne, Marco Andreani, Manuela Testi, Mathijs Groeneweg, Marcel G J Tilanus
The gold standard for typing at the allele level of the highly polymorphic Human Leucocyte Antigen (HLA) gene system is sequence based typing. Since sequencing strategies have mainly focused on identification of the peptide binding groove, full-length sequence information is lacking for >90% of the HLA alleles. One of the goals of the 17th IHIWS workshop is to establish full-length sequences for as many HLA alleles as possible. In our component "Extension of HLA sequences by full-length HLA allele-specific hemizygous Sanger sequencing" we have used full-length hemizygous Sanger Sequence Based Typing to achieve this goal...
August 11, 2018: Human Immunology
Maria Luiza Guimarães de Oliveira, Luciana Caricati Veiga-Castelli, Letícia Marcorin, Guilherme Debortoli, Alison Luis Eburneo Pereira, Nádia Carolina de Aguiar Fracasso, Guilherme do Valle Silva, Andréia S Souza, Juliana Doblas Massaro, Aguinaldo Luiz Simões, Audrey Sabbagh, Eduardo Antônio Donadi, Erick C Castelli, Celso Teixeira Mendes-Junior
Human leukocyte antigen-G (HLA-G) is a nonclassical Major Histocompatibility Complex (MHC) molecule with immunomodulatory function and restricted tissue expression. The genetic diversity of HLA-G has been extensively studied in several populations, however, the segment located upstream -1406 has not yet been evaluated. We characterized the nucleotide variation and haplotype structure of an extended distal region (-2635), all exons and the 3'UTR segment of HLA-G by next-generation sequencing (NGS) in a sample of 335 Brazilian individuals...
August 11, 2018: Human Immunology
Ann M Dixon, Syamal Roy
Class II Major Histocompatibility complex (MHC-II) is a polymorphic heterodimer that binds antigen-derived peptides and presents them on the surface of antigen presenting cells. This mechanism of antigen presentation leads to recognition by CD4 T-cells and T-cell activation, making it a critical element of adaptive immune response. For this reason, the structural determinants of MHC-II function have been of great interest for the past 30 years, resulting in a robust structural understanding of the extracellular regions of the complex...
August 10, 2018: Human Immunology
Jonathan A Harton
In addition to their role in antigen presentation, class II MHC molecules also transmit signals to B lymphocytes. Class II MHC-mediated signals initiate a range of events in B cells, including induction of cell surface proteins, initiation of cell-cycle progression/proliferation, activation of or protection from apoptosis, and antigen-dependent plasma cell differentiation. Although various transmembrane signaling proteins associate with class II MHC molecules, the class II MHC cytoplasmic domains are essential for signals leading to increased intracellular cAMP and activation of protein kinase C (PKC)...
July 26, 2018: Human Immunology
Tao Liang, Yi Ting Xu, Yang Zhang, Peng Cheng Cai, Li Hua Hu
Interleukin 17 (IL-17) plays important roles in the progression of asthma. Genetic variants in the Il-17 may influence the immunopathogenesis of many diseases. Many studies have investigated the relevance of IL-17 polymorphism with cancers or immune diseases, including asthma. In this study, single nucleotide polymorphisms (SNPs) of IL-17 were explored by PCR-RFLP and verified by sequencing method. The frequencies of genotypes and alleles were analyzed. Haplotypes were analyzed with the SHEsis online program...
July 20, 2018: Human Immunology
Bibiana Sgorla de Almeida, Yara Costa Netto Muniz, Alice Heidrich Prompt, Erick C Castelli, Celso Teixeira Mendes-Junior, Eduardo Antonio Donadi
BACKGROUND: HLA-G is an immune checkpoint molecule. Since a differential molecule expression has been reported even for healthy individuals, many studies have focused on polymorphisms at HLA-G regulatory regions, particularly the 3' untranslated region (3'UTR). The presence/absence of a 14-bp sequence was the first polymorphism described and it is the most studied in association between HLA-G and disorders. METHODS: In this study, we performed a systematic review and meta-analysis of all association studies published regarding the HLA-G 14-bp...
October 2018: Human Immunology
Elena Kuzminova, Ekaterina Khamaganova, Tatiana Gaponova, Valeriy Savchenko
This report shows the HLA-A, -B, -C, -DRB1 and -DQB1 allele and haplotype frequencies in a population of 127 healthy Ossetian donors of blood marrow from Vladikavkaz, Russia. First- and second-field (for HLA-C locus) HLA genotyping was performed by polymerase chain reaction sequence-specific priming and/or oligonucleotide probes. Statistical analysis were performed using gene counting and Arlequin software packages. There was no deviation from Hardy-Weinberg equilibrium for all tested loci. The HLA genotypic and haplotypic data of the Ossetians reported here are available in free access at the Allele Frequencies Net Database (http://www...
October 2018: Human Immunology
Harold C Sullivan, Christina L Dean, Robert S Liwski, Shilpee Biswas, Abigail L Goodman, Scott Krummey, Howard M Gebel, Robert A Bray
Flow cytometric crossmatches (FCXM) are routinely performed to support living-donor renal transplantation. While long a laboratory mainstay, a physical crossmatch is costly, time consuming, and frequently poses interpretative conundrums with both false-positive and false- negative results. Given the increased utilization of the virtual crossmatch (vXM) in the deceased donor setting, our aim was to assess its utility in living donor evaluations. We reviewed 100 living donor FCXMs and retrospectively performed a vXM for each pair...
October 2018: Human Immunology
Alexander Queck, Sabrina Rueschenbaum, Alica Kubesch, Chengcong Cai, Stefan Zeuzem, Andreas Weigert, Bernhard Brüne, Nour-Eldin A Nour-Eldin, Tatjana Gruber-Rouh, Thomas Vogl, Christian M Lange
Advanced liver diseases are associated with impaired intestinal barrier function, which results in bacterial influx via the portal vein to the liver, causing hepatic and systemic inflammation. Little is known about possible concomitant trafficking of immune cells from the intestines to the liver. We therefore performed a comprehensive immunophenotyping study of the portal venous versus peripheral blood compartment in patients with liver cirrhosis who received a transjugular intrahepatic portosystemic stent shunt (TIPS)...
October 2018: Human Immunology
Steven G E Marsh
No abstract text is available yet for this article.
October 2018: Human Immunology
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