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Biotechnology Letters

Zihang Li, Junli Zhao, Niaz Muhammad, Dongyang Wang, Qinwen Mao, Haibin Xia
OBJECTIVES: To establish a HEK293 cell line with a luciferase knock-in reporter controlled by the endogenous SREBP1 promoter for investigating transcriptional regulation of the SREBP1 gene. RESULTS: PCR confirmed the site-specific integration of a single copy of the exogenous luciferase gene into one allele of the genome and a 14 bp deletion of the targeted sequence in the other. Luciferase activity was directly correlated with the promoter activity of the endogenous SREBP1 gene in the HEK293-SREBP1-T2A-luciferase-KI cell line cell line...
September 19, 2018: Biotechnology Letters
Mengjuan Liu, Jiaqi Wang, Hongping Tang, Li Fan, Liang Zhao, Hai-Bin Wang, Yan Zhou, Wen-Song Tan
OBJECTIVE: To explore the impact of taurine on monoclonal antibody (mAb) basic charge variants in Chinese hamster ovary (CHO) cell culture. RESULTS: In fed-batch culture, adding taurine in the feed medium slightly increased the maximum viable cell density and mAb titers in CHO cells. What's more, taurine significantly decreased the lysine variant and oxidized variant levels, which further decreased basic variant contents from 32 to 27%. The lysine variant content in the taurine culture was approximately 4% lower than that in control condition, which was the main reason for the decrease in basic variants...
September 18, 2018: Biotechnology Letters
Jayanthi Nagappan, Chiew Foan Chin, Lee Pei Lee Angel, Richard M Cooper, Sean T May, Eng-Ti Leslie Low
The first and most crucial step of all molecular techniques is to isolate high quality and intact nucleic acids. However, DNA and RNA isolation from fungal samples are usually difficult due to the cell walls that are relatively unsusceptible to lysis and often resistant to traditional extraction procedures. Although there are many extraction protocols for Ganoderma species, different extraction protocols have been applied to different species to obtain high yields of good quality nucleic acids, especially for genome and transcriptome sequencing...
September 10, 2018: Biotechnology Letters
Lihao Ge, Yuyan Wang, Yang Cao, Gang Li, Rui Sun, Peng Teng, Yansong Wang, Yunlong Bi, Zhanpeng Guo, Yajiang Yuan, Deshui Yu
MicroRNA-429(miR-429) plays an important role in mesenchymal stem cells. Hypoxia-inducible factor 1α (HIF-1α) is a nuclear transcription factor that regulates the proliferation, apoptosis and tolerance to hypoxia of mesenchymal stem cells. HIF-1α is also a target gene of miR-429. We investigated whether miR-429 plays a role in hypoxia tolerance with HIF-1α in human amniotic mesenchymal stem cells (hAMSCs). The expression of miR-429 was increased by hypoxia in hAMSCs. miR-429 expression resulted in decreased HIF-1α protein level, but little effect on HIF-1α mRNA...
August 25, 2018: Biotechnology Letters
Lakshmi Tripathi, Victor U Irorere, Roger Marchant, Ibrahim M Banat
Surfactants and emulsifiers are surface-active compounds (SACs) which play an important role in various industrial processes and products due to their interfacial properties. Many of the chemical surfactants in use today are produced from non-renewable petrochemical feedstocks, while biosurfactants (BS) produced by microorganisms from renewable feedstocks are considered viable alternatives to petroleum based surfactants, due to their biodegradability and eco-friendly nature. However, some well-characterised BS producers are pathogenic and therefore, not appropriate for scaled-up production...
August 25, 2018: Biotechnology Letters
Hala S Helal, Amro Hanora, Rania Abdelmonem Khattab, Hayam Hamouda, Hamdallah Zedan
OBJECTIVE: The objective of this work was to isolate bacteria from Red Sea invertebrates, determine their antimicrobial activity, and screen for the biosynthetic gene clusters [polyketides (PKs) and nonribosomal peptides (NRPs)] which could be involved in the production of bioactive secondary metabolites. RESULT: Eleven different samples of marine invertebrates' were collected from Egypt's Red Sea (El-Tor-Sharm El-Sheikh and Hurghada) by scuba diving, and a total 80 isolates of the associated microorganisms were obtained from the cultivation on six different cultural medium...
August 17, 2018: Biotechnology Letters
Zuleyka S Oros-Flores, Luz E Casados-Vázquez, Dennis K Bideshi, Rubén Salcedo-Hernández, José E Barboza-Corona
OBJECTIVES: To develop a recombinant strain of Bacillus thuringiensis that synthesizes two bacteriocins that enhance the antibacterial potency of the strain and that could have applied clinical and industrial value. RESULTS: We cloned the thurincin H cluster into the pHT3101 vector by assembling two genetic cassettes harboring genes for the synthesis, modification, immunity and transport of thurincin H. This construct was used to transform a thurincin H-sensitive strain of B...
August 13, 2018: Biotechnology Letters
Jaeeun Song, Su-Yeon Kim, Dae-Hyuk Kim, Young-Seok Lee, Joon-Soo Sim, Bum-Soo Hahn, Chang-Muk Lee
OBJECTIVE: Hermetia illucens is a voracious insect scavenger that efficiently decomposes food waste. To exploit novel hydrolytic enzymes from this insect, we constructed a fosmid metagenome library using unculturable H. illucens intestinal microorganisms. RESULTS: Functional screening of the library on carboxymethyl cellulose plates identified a fosmid clone with a product displaying hydrolytic activity. Fosmid sequence analysis revealed a novel mannan-degrading gene (ManEM17) composed of 1371 base pairs, encoding 456 amino acids with a deduced 54 amino acid N-terminal signal peptide sequence...
August 4, 2018: Biotechnology Letters
Paula Istvan, Amanda Araújo Souza, Aisel Valle Garay, Debora Farage Knupp Dos Santos, Gideane Mendes de Oliveira, Renata Henrique Santana, Fabyano Alvares Cardoso Lopes, Sonia Maria de Freitas, João Alexandre Ribeiro Gonçalves Barbosa, Ricardo Henrique Krüger
OBJECTIVE: To isolate putative lipase enzymes by screening a Cerrado soil metagenomic library with novel features. RESULTS: Of 6720 clones evaluated, Clone W (10,000 bp) presented lipolytic activity and four predicted coding sequences, one of them LipW. Characterization of a predicted esterase/lipase, LipW, showed 28% sequence identity with an arylesterase from Pseudomonas fluorescens (pdb|3HEA) from protein database (PDB). Phylogenetic analysis showed LipW clustered with family V lipases; however, LipW was clustered in different subclade belonged to family V, suggesting a different subgroup of family V...
July 30, 2018: Biotechnology Letters
Mishal Subhan, Rani Faryal, Ian Macreadie
OBJECTIVES: Reduced efficacy of statins has been observed in people but the mechanism of this resistance is unclear and no statin-resistance mutations in the catalytic domain of HMGCR have been reported. The present study focused on looking for statin-resistance mutations and examining the mechanism of statin resistance using Candida glabrata as a model organism. RESULTS: C. glabrata was cultured in media containing lovastatin, simvastatin or atorvastatin to obtain lovastatin-, simvastatin- and atorvastatin-resistant mutants...
July 27, 2018: Biotechnology Letters
Liu Yang, Nandi Zhou, Yaping Tian
OBJECTIVE: We aimed to identify new high-yield dextranase strains and study the catalytic potential of dextranase from the strain in industrial applications. RESULTS: Dextranase-producing strains were screened from soil samples, and a potential strain was identified as Chaetomium globosum according to its phenotype, biochemical characteristics, and rDNA analysis. Crude dextranase was purified to reach 10.97-fold specific activity and 18.7% recovery. The molecular weight of the enzyme was 53 kDa with an optimum temperature and pH of 60 °C and 5...
July 27, 2018: Biotechnology Letters
Xi Gao, Bo Kong, R Dennis Vigil
Widespread cultivation of phototrophic microalgae for sustainable production of a variety of renewable products, for wastewater treatment, and for atmospheric carbon mitigation requires not only improved microorganisms but also significant improvements to process design and scaleup. The development of simulation tools capable of providing quantitative predictions for photobioreactor performance could contribute to improved reactor designs and it could also support process scaleup, as it has in the traditional petro-chemical industries...
July 26, 2018: Biotechnology Letters
Qianqian Ji, Caiping Guo, Chen Xie, Yingdan Wu, Pei Zhang, Hui Li, Yongjun Lu
In the original publication of the article, the Acknowledgement section was published incompletely. The complete Acknowledgement is given in this Correction.
July 23, 2018: Biotechnology Letters
Haixia Li, Qian Guo
In the original publication of the article, the second author name was published incorrectly. The corrected author name is given in this correction.
July 21, 2018: Biotechnology Letters
Mark Schofield
There is an upsurge of interest in continuous bioprocessing, but currently continuous downstream bioprocessing has not been implemented to generate clinical material. This review focusses on the current state of the art of continuous downstream processing, highlighting the key advantages over traditional batch manufacturing. This allows the identification of scenarios where continuous downstream processing may be critical for commercial manufacturing success.
July 13, 2018: Biotechnology Letters
Jinxiang Zhu, Qiaoyun Zhu, Ruiqing Gong, Qin Xu, Menghao Cai, Tianyi Jiang, Xiangshan Zhou, Mian Zhou, Yuanxing Zhang
OBJECTIVE: Around one-fourth of the Komagataella phaffii genes encode hypothetical proteins with unknown functions. However, lack of powerful tools for genetic screening in K. phaffii significantly limits the functional analysis of these unknown genes. Transposon mutagenesis has been utilized as an insertional mutagenesis tool in many other organisms and would be extremely valuable if it could be applied in K. phaffii. RESULTS: In this study, we investigated in K...
July 12, 2018: Biotechnology Letters
Salvatore La China, Gabriele Zanichelli, Luciana De Vero, Maria Gullo
Acetic acid bacteria are versatile organisms converting a number of carbon sources into biomolecules of industrial interest. Such properties, together with the need to limit chemical syntheses in favor of more sustainable biological processes, make acetic acid bacteria appropriate organisms for food, chemical, medical, pharmaceutical and engineering applications. At current, well-established bioprocesses by acetic acid bacteria are those derived from the oxidative pathways that lead to organic acids, ketones and sugar derivates...
July 10, 2018: Biotechnology Letters
Yun Xue, Mengya Wang, Pengchao Zhao, Chunshan Quan, Xin Li, Lina Wang, Weina Gao, Jinghua Li, Xiangyang Zu, Dongliao Fu, Shuxiao Feng, Ping Li
Gram-negative bacilli such as Pseudomonas spp., Pseudoalteromonas sp., Angiococcus sp., Archangium sp., Burkholderia spp., Chromobacterium sp., Chondromyces sp., Cystobacter sp., Jahnella sp., Janthinobacterium sp., Lysobacter spp., Paraliomyxa sp., Photobacterium spp., Photorhabdus sp., Pontibacter sp., Ruegeria sp., Serratia sp., Sorangium sp., Sphingomonas sp., and Xenorhabdus spp. produce an enormous array of short peptides of 30 residues or fewer that are potential pharmaceutical drugs and/or biocontrol agents...
July 2, 2018: Biotechnology Letters
Yixing Zhang, Makoto Yoshida, Praveen V Vadlani
D-lactic acid is a versatile and important industrial chemical that can be applied in the synthesis of thermal-resistant poly-lactic acid. Biosynthesis of D-lactic acid can be achieved by a variety of microorganisms, including lactic acid bacteria, yeast, and fungi; however, the final product yield, optical purity, and the utilization of both glucose and xylose are restricted. Consequently, engineered microbial systems are essential to attain high titer, productivity, and complete utilization of sugars. Herein, we critically evaluate the promising wild-type microorganisms, as well as genetically modified microorganisms to produce enantiomerically pure D-lactic acid, particularly from renewable lignocellulosic biomass...
August 2018: Biotechnology Letters
P Castellano, M Pérez Ibarreche, L Longo Borges, F C Niño Arias, G R Ross, E C Pereira De Martinis
OBJECTIVES: The objective of this study was to evaluate the ability of Lactobacillus curvatus CRL705, CRL1532, and CRL1533 and Lactobacillus sakei CRL1613 to survive under simulated gastrointestinal conditions. Moreover, a microencapsulation approach was proposed to improve gastrointestinal survival. Finally, experiments were performed to demonstrate that Lactobacillus spp. can modulate the ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells. RESULTS: Lactobacillus strains were encapsulated in alginate beads to enhance the survival of bacteria under in vitro gastrointestinal conditions...
August 2018: Biotechnology Letters
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