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Biotechnology Letters

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https://www.readbyqxmd.com/read/28721587/genetically-engineered-cell-lines-for-%C3%AE-1-antitrypsin-expression
#1
Qianqian Ji, Caiping Guo, Chen Xie, Yingdan Wu, Pei Zhang, Hui Li, Yongjun Lu
OBJECTIVES: To establish genetically modified cell lines that can produce functional α1-antitrypsin (AAT), by CRISPR/Cas9-assisted homologous recombination. RESULTS: α1-Antitrypsin deficiency (AATD) is a monogenic heritable disease that often results in lungs and liver damage. Current augmentation therapy is expensive and in short of supply. To develop a safer and more effective therapeutic strategy for AATD, we integrated the AAT gene (SERPINA1, NG_008290.1) into the AAVS1 locus of human cell line HEK293T and assessed the safety and efficacy of CRISPR/Cas9 on producing potential therapeutic cell lines...
July 18, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28721586/homology-modeling-and-prediction-of-the-amino-acid-residues-participating-in-the-transfer-of-acetyl-coa-to-arylalkylamine-by-the-n-acetyltransferase-from-chryseobacterium-sp
#2
Shinji Takenaka, Takahiro Ozeki, Kosei Tanaka, Ken-Ichi Yoshida
OBJECTIVES: To predict the amino acid residues playing important roles in acetyl-CoA and substrate binding and to study the acetyl group transfer mechanism of Chryseobacterium sp. 5-3B N-acetyltransferase (5-3B NatA). RESULTS: A 3-dimensional homology model of 5-3B NatA was constructed to compare the theoretical structure of this compound with the structures of previously reported proteins belonging to the bacterial GCN5 N-acetyltransferase family. Homology modeling of the 5-3B NatA structure and a characterization of the enzyme's kinetic parameters identified the essential amino acid residues involved in binding and acetyl-group transfer...
July 18, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28721585/cyp701a26-is-characterized-as-an-ent-kaurene-oxidase-with-putative-involvement-in-maize-gibberellin-biosynthesis
#3
Hongjie Mao, Qinqin Shen, Qiang Wang
OBJECTIVES: To characterize the ent-kaurene oxidase (KO) involved in maize (Zea mays) gibberellin (GA) biosynthesis. RESULTS: Two putative KO genes were identified in maize based on the homologous alignment. Biochemical characterization indicated that one of them encoded a cytochrome P450 monooxygenase (P450) CYP701A26, which reacted with ent-kaurene to form ent-kaurenoic acid, the key intermediate of GA biosynthesis. CYP701A26 showed constitutive expression in active growing tissues and no inducible expression, which led to putative designation of CYP701A26 as the ZmKO...
July 18, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28721584/mir-34a-attenuates-glioma-cells-progression-and-chemoresistance-via-targeting-pd-l1
#4
Yi Wang, Li Wang
OBJECTIVE: To investigate the roles of miR-34a in progression and chemoresistance of glioma cells. RESULTS: Quantitative real-time PCR analysis showed that miR-34a level was lower, but PD-L1 expression level was higher in glioma tissue specimens compared with normal brain tissues and their expression levels were negatively correlated. Ectopic expression of miR-34a inhibited glioma cell proliferation, promoted cell cycle arrest in G1/S phase and cell apoptosis. Additionally, miR-34a/PD-L1 axis was again confirmed and co-expression of PD-L1 with miR-34a mimics attenuated the effects of miR-34a on cell proliferation and apoptosis in glioma cells...
July 18, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28721583/microbial-and-genetically-engineered-oils-as-replacements-for-fish-oil-in-aquaculture-feeds
#5
REVIEW
M Sprague, M B Betancor, D R Tocher
As the global population grows more of our fish and seafood are being farmed. Fish are the main dietary source of the omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA), eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, but these cannot be produced in sufficient quantities as are now required for human health. Farmed fish have traditionally been fed a diet consisting of fishmeal and fish oil, rich in n-3 LC-PUFA. However, the increase in global aquaculture production has resulted in these finite and limited marine ingredients being replaced with sustainable alternatives of terrestrial origin that are devoid of n-3 LC-PUFA...
July 18, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28721582/mir-130b-3p-301b-3p-negatively-regulated-rb1cc1-expression-on-myogenic-differentiation-of-chicken-primary-myoblasts
#6
Jian Xue, Jingwen Xue, Ji Zhang, Dan Li, Lei Jiang
OBJECTIVES: To explore the roles of miR-130b-3p and miR-301b-3p which may regulate Rb1-inducible coiled-coil 1 (Rb1cc1) expression during myogenic differentiation of chicken primary myoblasts. RESULTS: After 4 days of myogenic differentiation, myotubes appeared and after 6 days the cells fused to each other and expression of MyHC could be detected by immunofluorescence staining. TargetScan and RNAhybrid 2.2 showed miR-130b-3p and miR-301b-3p were well complementary with the target site of Rb1cc1 3'-untranslated region (3'-UTR)...
July 18, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28721581/autophagy-regulated-by-lncrna-hotair-contributes-to-the-cisplatin-induced-resistance-in-endometrial-cancer-cells
#7
Meng-Yao Sun, Jian-Yong Zhu, Chun-Yan Zhang, Miao Zhang, Ya-Nan Song, Khalid Rahman, Li-Jun Zhang, Hong Zhang
OBJECTIVES: To identify whether lncRNAs (long non-coding RNA) participate in the regulation of cisplatin-resistant induced autophagy in endometrial cancer cells. RESULTS: Autophagy activity was significantly boosted in cisplatin-resistant Ishikawa cells, a human endometrial cancer cell line, compared with that in parental Ishikawa cells. After analyzing the overall long noncoding RNA (lncRNA) profiling, a meaningful lncRNA, HOTAIR, was identified. It was down-regulated simultaneously in cisplatin-resistant Ishikawa cells and parental Ishikawa cells treated with cisplatin...
July 18, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28721580/gene-expression-profiles-of-the-thermotolerant-yeast-saccharomyces-cerevisiae-strain-kku-vn8-during-high-temperature-ethanol-fermentation-using-sweet-sorghum-juice
#8
Atiya Techaparin, Pornthap Thanonkeo, Preekamol Klanrit
OBJECTIVES: To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions. RESULTS: The maximal ethanol concentration obtained by S. cerevisiae KKU-VN8 using SSJ at 40 °C was 66.6 g/l, with a productivity of 1.39 g/l/h and a theoretical ethanol yield of 81%...
July 18, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28676939/expression-purification-and%C3%A2-characterization-of-a-membrane-bound-d-amino-acid-dehydrogenase-from-proteus-mirabilis-jn458
#9
Jinjin Xu, Yajun Bai, Taiping Fan, Xiaohui Zheng, Yujie Cai
OBJECTIVES: To characterize a novel membrane-bound D -amino acid dehydrogenase from Proteus mirabilis JN458 (PmDAD). RESULTS: The recombinant PmDAD protein, encoding a peptide of 434 amino acids with a MW of 47.7 kDa, exhibited broad substrate specificity with D -alanine the most preferred substrate. The K m and V max values for D -alanine were 9 mM and 20 μmol min(-1) mg(-1), respectively. Optimal activity was at pH 8 and 45 °C. Additionally, this PmDAD generated H2O2 and exhibited 68 and 60% similarity with E...
July 4, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28667418/diabetic-nephropathy-serum-mir-9-confers-a-poor-prognosis-in-and-is-associated-with-level-changes-of-vascular-endothelial-growth-factor-and-pigment-epithelium-derived-factor
#10
Yanxin Xiao, Shuqin Guo, Yunliang Zhang, Zhiying Bian, Liyan Jia, Yanyun Hu, Jie Chen, Chao Yin, Ning Li, Dongxun Zhang, Xincui Zhao, Jun Wang
OBJECTIVE: To investigate the relationship between the serum level of miR-9 and the progression of diabetic nephropathy (DN) and related molecular mechanisms. RESULTS: Thirty-five healthy subjects and 140 DN patients were divided into five groups: control, DN I-II, DN III, DN IV and DN V. Serum level of miR-9 was measured by real-time qPCR. Serum levels of vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF) lipids, fasting glucose, insulin, hemoglobin A1c (HBA1c), creatinine, fibrinogen and insulin resistance (HOMA-IR) were also measured...
June 30, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28667417/enhanced-biomass-and-oxidative-stress-tolerance-of-synechococcus-elongatus-pcc-7942-overexpressing-the-dhar-gene-from-brassica-juncea
#11
Young-Saeng Kim, Il-Sup Kim, Joseph S Boyd, Arnaud Taton, James W Golden, Ho-Sung Yoon
OBJECTIVES: To improve the oxidative stress tolerance, biomass yield, and ascorbate/dehydroascorbate (AsA/DHA) ratio of Synechococcus elongatus PCC 7942 in the presence of H2O2, by heterologous expression of the dehydroascorbate reductase (DHAR) gene from Brassica juncea (BrDHAR). RESULTS: Under H2O2 stress, overexpression of BrDHAR in the transgenic strain (BrD) of S. elongatus greatly increased the AsA/DHA ratio. As part of the AsA recycling system, the oxidative stress response induced by reactive oxygen species was enhanced, and intracellular H2O2 level decreased...
June 30, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28664315/the-wound-healing-effect-of-7-3-4-trimethoxyflavone-through-increased-levels-of-prostaglandin-e2-by-15-hydroxyprostaglandin-dehydrogenase-inhibition
#12
Kyung Hoon Sun, Sandeep Karna, Young-Sook Moon, Hoon Cho, Cheol-Hee Choi
OBJECTIVE: To find an inhibitor of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) that rapidly metabolises Prostaglandin E2 (PGE2) as a mediator of wound healing, we examined seven flavonoids for this role. RESULTS: 7,3',4'-Trimethoxyflavone (TMF) had the lowest IC50 value of 0.34 µM for 15-PGDH inhibition but >400 µM for cytotoxicity, indicating a high therapeutic index. TMF elevated PGE2 levels in a concentration-dependent manner in both A549 lung cancer and HaCaT cells...
June 29, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28664314/avermectin-biosynthesis-stable-functional-expression-of-branched-chain-%C3%AE-keto-acid-dehydrogenase-complex-from-streptomyces-avermitilis-in-escherichia-coli-by-selectively-regulating-individual-subunit-gene-expression
#13
Qianqian Cui, Fengli Zhou, Weifeng Liu, Yong Tao
OBJECTIVE: To improve the production of short branched-chain acyl-CoAs for avermectin biosynthesis, the functional expression of the branched chain α-keto acid dehydrogenase complex (BKDH) from Streptomyces avermitilis was systematically optimized by selectively regulating individual subunit expression in Escherichia coli. RESULTS: Functional expression of the BKDH complex was achieved by independent and selective optimization of individual subunit genes of the complex...
June 29, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28664313/improving-the-power-generation-of-microbial-fuel-cells-by-modifying-the-anode-with-single-wall-carbon-nanohorns
#14
Jiawei Yang, Shaoan Cheng, Yi Sun, Chaochao Li
OBJECTIVES: To increase the power generation of microbial fuel cells (MFCs), anode modification with carbon materials (activated carbon, carbon nanotubes, and carbon nanohorns) was investigated. RESULTS: Maximum power densities of a stainless-steel anode MFC with a non-modified electrode (SS-MFC), an activated carbon-modified electrode (AC-MFC), a carbon nanotube-modified electrode (CNT-MFC) and a carbon nanohorn-modified electrode (CNH-MFC) were 72, 244, 261 and 327 mW m(-2), respectively...
June 29, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28660385/genetic-improvement-of-magnetospirillum-gryphiswaldense-for-enhanced-biological-removal-of-phosphate
#15
Yanping Zhou, Wiktor Lisowski, Yan Zhou, Ng Wun Jern, Kama Huang, Eileen Fong
OBJECTIVES: To improve its phosphate accumulating abilities for phosphate recycling from wastewater, a magnetotactic bacterium, Magnetospirillum gryphiswaldense, was genetically modified to over-express polyphosphate kinase. RESULTS: Polyphosphate kinase was over-expressed in the bacterium. The recombinant strain accumulated ninefold more polyphosphate from synthetic wastewater compared to original wild type. The magnetic property of the recombinant M. gryphiswaldense strain was retained...
June 28, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28653102/identification-of-set11-from-staphylococcus-aureus-mu50-strain-as-a-ortholog-of-ssl7-via-bioinformatic-analysis-and-determination-of-its-possible-targets-from-human-serum-using-cnbr-pull-down-assays
#16
Ying Li, Xiaobao Chen, Nan Jia, Xuan Zhang, Jianye Zang
OBJECTIVES: To identify and characterize staphylococcus exotoxin-like (SET) protein Set11 from Staphylococcus aureus Mu50 strain and its possible targets proteins from human blood/serum. RESULTS: Set11 is a member of the staphylococcal superantigen-like (SSL) proteins (also called Staphylococcus exotoxin-like (SET) proteins) family that is found in staphylococcal strain Mu50. Its structure and function, however, remain unknown. We performed bioinformatics analysis of Set11: it had 90% sequence identity to SSL7 in NCTC 8325 strain, indicating Set11 is a SSL7 ortholog...
June 26, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28634746/screening-and-identification-of-a-specific-peptide-binding-to-cervical-cancer-cells-from-a-phage-displayed-peptide-library
#17
Chunyan Li, Ning Gao, Qinqin Xue, Ni Ma, Yuqin Hu, Jianfang Zhang, Biliang Chen, Yingchun Hou
OBJECTIVES: To screen and identify the probe markers specifically binding to human cervical cancer, a phage-displayed 12-mer peptide library was used for biopanning of SiHa cells. RESULTS: After four rounds of whole-cell subtraction biopanning, the phage recovery was 21-fold higher (from 3.9 × 10(-5) to 8.3 × 10(-4)) than that of the first round, and specific phage clones were significantly enriched. 57 randomly selected phage clones were tested by ELISA, and 36 phage clones were identified as positive clones...
June 20, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28623443/autolyse-the-cell-in-order-to-save-it-inducing-then-blocking-autolysis-as-a-strategy-for-delaying-cell-death-in-the-probiotic-lactobacillus-reuteri
#18
Tahl Zimmerman, Rabin Gyawali, Salam Ibrahim
OBJECTIVE: To examine whether choline and its derivatives can be used to preserve viable cells of Lactobacillus reuteri in autolytic models. RESULTS: A phosphate-induced autolytic model in de Man, Rogosa and Sharpe medium (MRS) was used. Viable cell counts were determined by plated on MRS-agar. Choline and hemicholinium-3 (HC-3) significantly blocked autolysis of L. reuteri at 360 mM and 4 mM, respectively. Viable cell counts corroborated these observations. Importantly, autolytically induced cells treated with choline and hemicholinium-3 were significantly more viable then even non-induced cells...
June 16, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28612265/regular-enzyme-recovery-enhances-cellulase-production-by-trichoderma-reesei-in-fed-batch-culture
#19
Bucheng Wang, Lu Jiang, Hechao Bai, Qiang Yong, Shiyuan Yu
OBJECTIVE: To protect the enzymes during fed-batch cellulase production by means of partial enzyme recovery at regular intervals. RESULTS: Extracellular enzymes were partially recovered at the intervals of 1, 2, or 3 days. Mycelia were also removed to avoid contamination. Increases in the total harvested cellulase (24-62%) and β-glucosidase (22-76%) were achieved. In fermentor cultivation when the enzymes were recovered every day with 15% culture broth. The total harvested cellulase and β-glucosidase activity increased by 43 and 58%, respectively, with fungal cell concentration maintained at 3...
June 13, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28612264/application-of-recombinant-hemagglutinin-proteins-as-alternative-antigen-standards-for-pandemic-influenza-vaccines
#20
Yejin Choi, Seong Yi Kwon, Ho Jung Oh, Sunbo Shim, Seokkee Chang, Hye Joo Chung, Do Keun Kim, Younsang Park, Younghee Lee
OBJECTIVES: The single radial immunodiffusion (SRID) assay, used to quantify hemagglutinin (HA) in influenza vaccines, requires reference reagents; however, because centralized production of reference reagents may slow the emergency deployment of vaccines, alternatives are needed. RESULTS: We investigated the production of HA proteins using recombinant DNA technology, rather than a traditional egg-based production process. The HA proteins were then used in an SRID assay as a reference antigen...
June 13, 2017: Biotechnology Letters
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