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Biotechnology Letters

Feng Geng, Cheng-Wei Ma, An-Ping Zeng
OBJECTIVE: To re-engineer the active site of proteins for non-natural substrates using a position-based prediction method (PBPM). RESULTS: The approach has been applied to re-engineer the E. coli glutamate dehydrogenase to alter its substrate from glutamate to homoserine for a de novo 1,3-propanediol biosynthetic pathway. After identification of key residues that determine the substrate specificity, residue K92 was selected as a candidate site for mutation. Among the three mutations (K92V, K92C, and K92M) suggested by PBPM, the specific activity of the best mutant (K92 V) was increased from 171 ± 35 to 1328 ± 71 μU mg(-1)...
February 9, 2017: Biotechnology Letters
Shazia Rehman, Ejaz Aziz, Wasim Akhtar, Muhammad Ilyas, Tariq Mahmood
Plant proteinase inhibitor-II (PI-II) proteins are one of the promising defensive proteins that helped the plants to resist against different kinds of unfavorable conditions. Different roles for PI-II have been suggested such as regulation of endogenous proteases, modulation of plant growth and developmental processes and mediating stress responses. The basic knowledge on genetic and molecular diversity of these proteins has provided significant insight into their gene structure and evolutionary relationships in various members of this family...
February 9, 2017: Biotechnology Letters
Milan Polakovič, Juraj Švitel, Marek Bučko, Jaroslav Filip, Vilém Neděla, Marion B Ansorge-Schumacher, Peter Gemeiner
Viable microbial cells are important biocatalysts in the production of fine chemicals and biofuels, in environmental applications and also in emerging applications such as biosensors or medicine. Their increasing significance is driven mainly by the intensive development of high performance recombinant strains supplying multienzyme cascade reaction pathways, and by advances in preservation of the native state and stability of whole-cell biocatalysts throughout their application. In many cases, the stability and performance of whole-cell biocatalysts can be highly improved by controlled immobilization techniques...
February 8, 2017: Biotechnology Letters
Peilin Han, Qingbo Cui, Shulong Yang, Hao Wang, Peng Gao, Zhaozhu Li
OBJECTIVES: To investigate the effects of tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) on the proliferation and differentiation of tendon-derived stem cells (TDSC). RESULTS: TNF-α inhibits the proliferation and tenogenic/osteogenic differentiation of TDSC but, after simultaneous or sequential treatment with TGF-β1 and TNF-α, the expression of tenogenic/osteogenic-related marker and proliferation of TDSC was significantly increased...
February 2, 2017: Biotechnology Letters
Han Bin Pek, Pei Yu Lim, Chengcheng Liu, Dong-Yup Lee, Xuezhi Bi, Fong Tian Wong, Dave Siak-Wei Ow
OBJECTIVES: To evaluate the secretory and cytoplasmic expression of a thermostable Thermogata maritima invertase in Lactococcus lactis. RESULTS: The thermostable invertase from T. maritima was cloned with and without the USP45 secretory peptide into the pNZ8148 vector for nisin-inducible expression in L. lactis. The introduction of an USP45 secretion peptide at the N-terminal of the enzyme led to a loss of protein solubility. Computational homology modeling and hydrophobicity studies indicated that the USP45 peptide exposes a stretch of hydrophobic amino acids on the protein surface resulting in lower solubility...
February 2, 2017: Biotechnology Letters
Elvis Legala Ongey, Hüseyin Yassi, Stephan Pflugmacher, Peter Neubauer
No abstract text is available yet for this article.
February 2, 2017: Biotechnology Letters
Zhaowei Yan, Sheng Ma, Yan Zhang, La Ma, Feng Wang, Jian Li, Liyan Miao
OBJECTIVES: To study the structure of a small GTPaseRhoA in complex with PDZRhoGEF and the inhibitor HL47, and to provide an easier template for R&D of RhoA inhibitor. RESULTS: Our initial attempts to obtain a binary complex of RhoA with the inhibitor HL47 were unsuccessful probably due to the presence of GDP. By targeting a ternary complex involving the RhoA-specific guanine nucleotide exchange factor PDZRhoGEF, we eliminated GDP and obtained a 2.3 Å structure of the RhoA-PDZRhoGEF-inhibitor HL47 ternary complex...
February 1, 2017: Biotechnology Letters
Ismail Eş, Amin Mousavi Khaneghah, Seyed Mohammad Bagher Hashemi, Mohamed Koubaa
Propionic acid and its derivatives are considered "Generally Recognized As Safe" food additives and are generally used as an anti-microbial and anti-inflammatory agent, herbicide, and artificial flavor in diverse industrial applications. It is produced via biological pathways using Propionibacterium and some anaerobic bacteria. However, its commercial chemical synthesis from the petroleum-based feedstock is the conventional production process bit results in some environmental issues. Novel biological approaches using microorganisms and renewable biomass have attracted considerable recent attention due to economic advantages as well as great adaptation with the green technology...
February 1, 2017: Biotechnology Letters
Alexandr E Urusov, Alina V Petrakova, Milyausha K Gubaydullina, Anatoly V Zherdev, Sergei A Eremin, Dezhao Kong, Liqiang Liu, Chuanlai Xu, Boris B Dzantiev
OBJECTIVE: To develop a high-sensitivity immunochromatographic test for fumonisin B1 in plant extracts. RESULTS: Unlike conventional immunochromatographic tests, this assay is performed in two stages: competitive reaction with free specific antibodies and identifying immune complexes by their interaction with the anti-species antibody-conjugated gold nanoparticles. The use of a new geometry for the test strip membranes and a novel reagent application method ensures the proper order of these stages without additional manipulations...
February 1, 2017: Biotechnology Letters
Xudong Zhu, Bessembayev Arman, Ju Chu, Yonghong Wang, Yingping Zhuang
OBJECTIVES: To develop an efficient cost-effective screening process to improve production of glucoamylase in Aspergillus niger. RESULTS: The cultivation of A. niger was achieved with well-dispersed morphology in 48-deep-well microtiter plates, which increased the throughput of the samples compared to traditional flask cultivation. There was a close negative correlation between glucoamylase and its pH of the fermentation broth. A novel high-throughput analysis method using Methyl Orange was developed...
January 30, 2017: Biotechnology Letters
Jhansi L Varanasi, Pallavi Sinha, Debabrata Das
OBJECTIVE: To selectively enrich an electrogenic mixed consortium capable of utilizing dark fermentative effluents as substrates in microbial fuel cells and to further enhance the power outputs by optimization of influential anodic operational parameters. RESULTS: A maximum power density of 1.4 W/m(3) was obtained by an enriched mixed electrogenic consortium in microbial fuel cells using acetate as substrate. This was further increased to 5.43 W/m(3) by optimization of influential anodic parameters...
January 28, 2017: Biotechnology Letters
Dongping Mo, Daheng Yang, Xuelian Xiao, Ruihong Sun, Lei Huang, Jian Xu
OBJECTIVE: To investigate the roles of miR-145 in lung adenocarcinoma (LAC) and to clarify the regulation of N-cadherin by miR-145. RESULTS: In 57 paired clinical LAC tissues, diminished miR-145 was significantly correlated with the lymph node metastasis and was negatively correlated with N-cadherin mRNA level expression. Wound healing and transwell assays revealed a reduced capability of tumor metastasis induced by miR-145 in LAC. miR-145 negatively regulated the invasion of cell lines through targeting N-cadherin by directly binding to its 3'-untranslated region...
January 24, 2017: Biotechnology Letters
Sebastiana Roccuzzo, Andrew P Beckerman, Jagroop Pandhal
No abstract text is available yet for this article.
January 24, 2017: Biotechnology Letters
Vahid Jalali Javaran, Alireza Shafeinia, Mokhtar Jalali Javaran, Esmaeil Ghasemi Gojani, Malihe Mirzaee
OBJECTIVE: To use a transient expression system to express a truncated human tissue plasminogen activator (K2S) gene in cucurbit plants. RESULTS: The recombinant tissue plasminogen activator protein (K2S form) was expressed in active form in cucurbit plants. Its molecular weight was 43 kDa. The plant-derived rt-PA was determined using goat anti-rabbit antibody by western blotting. Among the infected lines, the highest expression of rt-PA was 62 ng/100 mg per leaf tissue as measured by ELISA...
January 16, 2017: Biotechnology Letters
Hyo Sang Jo, Eun Ji Yeo, Min Jea Shin, Yeon Joo Choi, Hyeon Ji Yeo, Su Bin Cho, Jung Hwan Park, Chi Hern Lee, Won Sik Eum, Soo Young Choi
OBJECTIVES: To identify the protective effect of DJ-1 protein against oxidative stress-induced HepG2 cell death, we used cell-permeable wild type (WT) and a mutant (C106A Tat-DJ-1) protein. RESULTS: By using western blotting and fluorescence microscopy, we observed WT and C106A Tat-DJ-1 proteins were efficiently transduced into HepG2 cells. Transduced WT Tat-DJ-1 proteins increased cell survival and protected against DNA fragmentation and intracellular ROS generation levels in H2O2-exposed HepG2 cells...
January 10, 2017: Biotechnology Letters
Xianzhang Jiang, Hongjiao Liu, Yongchao Niu, Feng Qi, Mingliang Zhang, Jianzhong Huang
No abstract text is available yet for this article.
January 10, 2017: Biotechnology Letters
Chenghong Wang, Jiguo Qiu, Youjian Yang, Jinwei Zheng, Jian He, Shunpeng Li
OBJECTIVE: To identify and characterize a novel aryloxyphenoxypropionate (AOPP) herbicide-hydrolyzing carboxylesterase from Aquamicrobium sp. FPB-1. RESULTS: A carboxylesterase gene, fpbH, was cloned from Aquamicrobium sp. FPB-1. The gene is 798 bp long and encodes a protein of 265 amino acids. FpbH is smaller than previously reported AOPP herbicide-hydrolyzing carboxylesterases and shares only 21-35% sequence identity with them. FpbH was expressed in Escherichia coli BL21(DE3) and the product was purified by Ni-NTA affinity chromatography...
January 5, 2017: Biotechnology Letters
Joon Serk Seo, Byung Sub Min, Yeon Jung Kim, Jong Moon Cho, Gi-Seong Kwon, Byeong-Pil Lim, Shin-Jae Chang, Dong-Il Kim
OBJECTIVES: To evaluate the characteristics of a novel human cell line, F2N78, including growth performance, physicochemical properties, and biological activity via direct comparison with CHO cells. RESULTS: The culture performance and physicochemical properties of antibodies produced from F2N78 and CHO cells were compared. For charge variants, antibodies produced from F2N78 cells contained a greater acidic charge variants than CHO cells. Regarding main glycoforms, degree of galactosylation was 52% in CT-A produced from F2N78 cells compared to CHO cells (37%)...
January 4, 2017: Biotechnology Letters
Lu-Lu Hou, Feng Liu, Xiaonan Zang, Xuecheng Zhang, Bangxiang He, Yating Ding, Xinwei Song, Dongfang Xiao, Haitao Wang
OBJECTIVES: To optimize the cultivation media for the growth rate of Haematococcus pluvialis and to study the transcription regulation of the algal nitrate reductase (NR), a key enzyme for nitrogen metabolism. RESULTS: The NR gene from H. pluvialis hd7 consists of 5636 nucleotides, including 14 introns. The cDNA ORF is 2718 bp, encoding a 905 aa protein with three conserved domains. The NR amino acids of H. pluvialis hd7 are hydrophilic and have similarity of 72% compared to that of Dunaliella...
January 4, 2017: Biotechnology Letters
Chunlong Sun, Hongtao Zhang, Wen Du, Baoqin Wang, Min Ji
OBJECTIVE: Methionine is a valid target for the treatment of cancer and to achieve in vivo imaging and early diagnosis of tumors, we have synthesized near-infrared (NIR) fluorochrome IR-822-labeled methionine (IR-822-Met). RESULTS: NIR fluorescent dye IR-822 was conjugated with methionine through its amide bond. It had low toxicity to normal cell/tissues. In vitro and in vivo studies demonstrated its high targeting capability to tumors. The results support the potential of using ligand-modified methionine probe for tumor diagnosis and targeted therapy...
January 3, 2017: Biotechnology Letters
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