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Biotechnology Letters

Guozeng Wang, Yaxin Ren, Tzi Bun Ng, Wolfgang R Streit, Xiuyun Ye
OBJECTIVE: To explore the diversity of glycoside hydrolase family 10 xylanase genes in the sediment of soda lake Dabusu by using high-throughput amplicon sequencing based on the Illumina HiSeq2500 platform. RESULTS: A total of 227,420 clean reads, representing approximately 49.5 M bp, were obtained. Operational taxonomic unit (OTU) classification, with a 95% sequence identity cut-off, resulted in 467 OTUs with 392 annotated as GH10 xylanase, exhibiting 35-99% protein sequence identity with their closest-related xylanases in GenBank...
January 14, 2019: Biotechnology Letters
Jing-Qiu Hua, Lei Yu
OBJECTIVES: This study reports the identification of a new bacterial azoreductase (AzoR) from Klebsiella oxytoca GS-4-08, its heterologous production in Escherichia coli and the decolorization of azo dyes. RESULTS: The AzoR, as a flavin-free, oxygen-insensitive enzyme, has a molecular mass of 22 kDa and a high substrate specificity for methyl red (MR). In the presence of 5 mM of NADH, the enzyme activity for decolorization of 50 mg l-1 of MR reached 5.61 μmol min-1 mg protein-1 ...
January 11, 2019: Biotechnology Letters
Razia Tahseen, Muhammad Arslan, Samina Iqbal, Zafar M Khalid, Muhammad Afzal
Soil contamination due to petroleum hydrocarbons is a ubiquitous environmental problem for which efficient remediation alternatives are required. Application of hydrocarbons degrading bacteria with enhanced degradation potential is such an alternative. The aim of present investigation was to induce mutagenicity in Pseudomonas putida through gamma-ray irradiation for the enhanced degradation of crude oil. A total of mutant 10 bacterial strains (300A-J) were screened for their degradation abilities in vitro; among which the performance of 300-B was outstanding...
January 4, 2019: Biotechnology Letters
Masayasu Mie, Takahiro Niimi, Yasumasa Mashimo, Eiry Kobatake
OBJECTIVE: We developed a DNA-NanoLuc luciferase (NnaoLuc) conjugates for DNA aptamer-based sandwich assay using the catalytic domain of the replication initiator protein derived from porcine circovirus type 2 (pRep). RESULTS: For construction of DNA aptamer and NanoLuc conjugate using the catalytic domain of Rep from PCV2. pRep fused to NanoLuc was genetically constructed and expressed in E. coli. After purification, the activities of fused pRep and NanoLuc were evaluated, and DNA-NanoLuc conjugates were constructed via the fused pRep...
January 3, 2019: Biotechnology Letters
Alexey P Tyunin, Nikolay N Nityagovsky, Yuri A Karetin, Konstantin V Kiselev
OBJECTIVE: Studies concerning 2b protein from Cucumovirus showed 2b to effectively repress functioning of the plant silencing complex, current study aimed whether retransformation with 2b gene able to restore silenced transgene expression in plant cells. RESULTS: A rolB-transgenic cell culture of Vitis amurensis Rupr. that was continuously subcultured during more than 10 years and exhibited decreased transcription of the rolB transgene was retransformed with the 2b gene of Cucumovirus-NK...
January 2, 2019: Biotechnology Letters
John A Renye, George A Somkuti, Dennis H Steinberg
OBJECTIVES: To demonstrate that S. thermophilus ST109 produces an antimicrobial peptide encoded within the bacteriocin-like peptide (blp) gene cluster, and to determine its broad spectrum activity against potential human pathogens. RESULTS: Analysis of the cell free supernatant (CFS) revealed that antimicrobial activity was associated with the presence of a heat-stable peptide of approximately 5-6 kDa; and activity was lost after protease treatment or exposure to α-amylase...
December 18, 2018: Biotechnology Letters
Dongchang Sun, Lin Wang, Xudan Mao, Mingyue Fei, Yiyang Chen, Minjia Shen, Juanping Qiu
OBJECTIVES: To develop a convenient chemical transformation mediated CRISPR/Cas9 (CT-CRISPR/Cas9) system for genome editing in Escherichia coli. RESULTS: Here, we have constructed a CT-CRISPR/Cas9 system, which can precisely edit bacterial genome (replacing, deleting, inserting or point mutating a target gene) through chemical transformation. Compared with the traditional electroporation mediated CRISPR/Cas9 (ET-CRISPR/Cas9) system, genome editing with the CT-CRISPR/Cas9 system is much cheaper and simpler...
December 13, 2018: Biotechnology Letters
Ganna Petruk, Mariana Roxo, Federica De Lise, Francesca Mensitieri, Eugenio Notomista, Michael Wink, Viviana Izzo, Daria Maria Monti
OBJECTIVE: The antioxidant activity and protective effect of a methanolic extract obtained from the marine Gram-negative bacterium Novosphingobium sp. PP1Y, isolated from the surface water of a polluted area in the harbour of Pozzuoli (Naples, Italy), was evaluated. RESULTS: The extract was tested in vitro on epithelial colorectal adenocarcinoma cells and in vivo on Caenorhabditis elegans. It showed strong protective activity against oxidative stress, in both experimental systems, by preventing ROS accumulation...
December 12, 2018: Biotechnology Letters
Zeeshan A Khan, Mohd F Siddiqui, Seungkyung Park
Antibiotic susceptibility test (AST) is an umbrella term for techniques to determine the susceptibility of bacteria to antibiotics. The antibiotic-resistant bacteria are a major threat to public health and a directed therapy based on accurate AST results is paramount in resistance control. Here we have briefly covered the progress of conventional, molecular, and automated AST tools and their limitations. Various aspects of microfluidic AST such as optical, electrochemical, colorimetric, and mechanical methods have been critically reviewed...
December 12, 2018: Biotechnology Letters
Ning Liao, Baolong Luo, Jing Gao, Xiaojuan Li, Zhixia Zhao, Yan Zhang, Yongqing Ni, Fengwei Tian
OBJECTIVES: Four kinds of oligosaccharides were used as co-encapsulating agents to test the effect of extrusion-based microencapsulation on protection of Lactobacillus fermentum L7 against exposure to simulated gastric and intestinal juices as well as long-term refrigeration storage at 4 °C. RESULTS: The combination of alginate with galacto-oligosaccharides, isomalto-oligosaccharides, fructo-oligosaccharides, and xylo-oligosaccharides, or alginate alone exhibited good properties of the beads...
December 10, 2018: Biotechnology Letters
Priyanka Priyanka, Yeqi Tan, Gemma K Kinsella, Gary T Henehan, Barry J Ryan
OBJECTIVE: This review examines on our current understanding of microbial lipase solvent tolerance, with a specific focus on the molecular strategies employed to improve lipase stability in a non-aqueous environment. RESULTS: It provides an overview of known solvent tolerant lipases and of approaches to improving solvent stability such as; enhancing stabilising interactions, modification of residue flexibility and surface charge alteration. It shows that judicious selection of lipase source supplemented by appropriate enzyme stabilisation, can lead to a wide application spectrum for lipases...
December 7, 2018: Biotechnology Letters
Marcela Robles-Machuca, M Martin Del Campo, M Ángeles Camacho-Ruiz, Enrique Ordaz, Edgar O Zamora-González, Marcelo Müller-Santos, Jorge A Rodríguez
OBJECTIVES: Ustilago maydis lipase A (UMLA) expressed in Pichia pastoris was compared with Candida antarctica lipase A (CALA) to study its biochemical properties such as thermostability and selectivity. RESULTS: UMLA had similar behavior to its homologue CALA regarding the effect of pH and temperature on enzymatic activity, substrate preference and selectivity. Both lipases were active on insoluble triglycerides as well as natural oils and hydrolyzed preferably esters with short and medium acyl and alkyl chains...
December 5, 2018: Biotechnology Letters
Manuela Panić, Doris Delač, Marin Roje, Ivana Radojčić Redovniković, Marina Cvjetko Bubalo
OBJECTIVE: Chiral building blocks [(S)-1-(3-methylphenyl)ethanol, (S)-1-(3,4-dimethylphenyl)ethanol and (S)-1-(2,4,6-trimethylphenyl)ethanol] for drug synthesis were prepared using two green approaches: (1) the yeast Saccharomyces cerevisiae as the biocatalyst and (2) the natural deep eutectic solvents (NADES) as the alternative solvents. Three different NADES with different water contents were prepared and screened for the highest conversion and enantiomeric excess of reduction of 1-(3-methylphenyl)ethanone, 1-(3,4-dimethylphenyl)ethanone (DMPA) and 1-(2,4,6-trimethyphenyl)ethanone by S...
December 3, 2018: Biotechnology Letters
Aline de Luna Marques, Ofélia de Queiroz Fernandes Araújo, Magali Christe Cammarota
Studies have reported enhancements in methane yield from pretreatment methods that benefit the anaerobic digestion (AD) of microalgae. However, energy return on investment (EROI), i.e., methane production enhancement achieved over energy input, may be unfavorable. Aiming to quantify EROI of AD microalgae pretreatment, about 180 experiments applied to 30 microalgae biomasses were compiled through an extensive literature survey, classified into 4 pretreatments (physical, enzymatic, chemical, and hybrid), and analyzed...
December 1, 2018: Biotechnology Letters
Roxana-Andreea Ilica, Lenuţa Kloetzer, Anca-Irina Galaction, Dan Caşcaval
Fumaric acid is a valuable compound used in foods, beverages, detergents, animal feed, pharmaceuticals and miscellaneous industrial products. It is produced on a large scale by the petrochemical route but the current tendency is towards implementing "green production" and environmental friendly technologies like biotechnological production of fumaric acid using low-cost raw materials. In this context, numerous studies focus on improving the fermentation process not only by using renewable raw material and genetically modified microorganisms, but also by developing and applying different downstream techniques for easy recovery of fumaric acid from the fermented broth...
December 1, 2018: Biotechnology Letters
Alan Costello, Nga T Lao, Niall Barron, Martin Clynes
The impact of local structure on mRNA translation is not well-defined pertaining to the 5' UTR. Reports suggest structural remodelling of the 5' UTR can significantly influence mRNA translation both in cis and trans however a new layer of complexity has been applied to this model with the now known reversible post-transcriptional chemical modification of RNA. N6 -methyladenosine (m6 A) is the most abundant internal base modification in mammalian mRNA. It has been reported that mRNAs harbouring m6 A motifs in their 5' UTR have improved translation efficiency...
November 30, 2018: Biotechnology Letters
Zijian Tan, Jing Zhao, Jiuzhou Chen, Deming Rao, Wenjuan Zhou, Ning Chen, Ping Zheng, Jibin Sun, Yanhe Ma
OBJECTIVE: To enhance the thermostability and deregulate the hemin inhibition of 5-aminolevulinic acid (ALA) synthase from Rhodopseudomonas palustris (RP-ALAS) by a computer-aided rational design strategy. RESULTS: Eighteen RP-ALAS single variants were rationally designed and screened by measuring their residual activities upon heating. Among them, H29R and H15K exhibited a 2.3 °C and 6.0 °C higher melting temperature than wild-type, respectively. A 6.7-fold and 10...
November 29, 2018: Biotechnology Letters
Chen Shen, Wanli Zhao, Xuming Liu, Jihua Liu
OBJECTIVES: To investigate the regio-selective demethylation of papaverine by CYP105D1 and develop a whole-cell biocatalytic system for the preparative synthesis of 6-O-demethyl-papaverine. RESULTS: CYP105D1 from Streptomyces griseus ATCC 13273 was used for the regioselective demethylation of papaverine at C-6 using putidaredoxin reductase (PDR) and putidaredoxin (Pdx) as the electron transport system. The Km value of CYP105D1 towards papaverine was estimated to be 92...
November 21, 2018: Biotechnology Letters
Hua Shao, Xianmei Hu, Liping Sun, Wenshan Zhou
OBJECTIVE: To obtain active lipases for biodiesel production by refolding Proteus sp. lipase inclusion bodies expressed in E. coli. RESULTS: A lipase gene lipPN1 was cloned from Proteus sp. NH 2-2 and expressed in E. coli BL21(DE3). Non-reducing SDS-PAGE revealed that recombinant LipPN1(rLipPN1) were prone to form inclusion bodies as disulfide-linked dimers in E. coli. Site-directed mutagenesis confirmed that Cys85 in LipPN1 was involved in the dimer formation. After optimizing the inclusion body refolding conditions, the maximum lipase activity reached 1662 U/L...
November 16, 2018: Biotechnology Letters
Cristiane Angélica Ottoni, Marta F Simões, Jonas G Santos, Luciana Peixoto, Cleiton R Martins, Bruno P Silva, Almir O Neto, António G Brito, Alfredo E Maiorano
OBJECTIVE: Our study evaluated the performance of different two-chambered microbial fuel cell (MFC) prototypes, operated with variable distance between electrodes and Nafion membrane and specific inoculum concentration, applied for vinasse treatment. RESULTS: The performance of the developed MFC resulted in a maximum current density of 1200 mA m-2 and power density of 800 mW m-2 in a period of 61 days. MFC performed a chemical oxygen demand removal at a rate ranging from 51 to 60%...
November 15, 2018: Biotechnology Letters
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