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Biotechnology Letters

Chunyan Li, Ning Gao, Qinqin Xue, Ni Ma, Yuqin Hu, Jianfang Zhang, Biliang Chen, Yingchun Hou
OBJECTIVES: To screen and identify the probe markers specifically binding to human cervical cancer, a phage-displayed 12-mer peptide library was used for biopanning of SiHa cells. RESULTS: After four rounds of whole-cell subtraction biopanning, the phage recovery was 21-fold higher (from 3.9 × 10(-5) to 8.3 × 10(-4)) than that of the first round, and specific phage clones were significantly enriched. 57 randomly selected phage clones were tested by ELISA, and 36 phage clones were identified as positive clones...
June 20, 2017: Biotechnology Letters
Tahl Zimmerman, Rabin Gyawali, Salam Ibrahim
OBJECTIVE: To examine whether choline and its derivatives can be used to preserve viable cells of Lactobacillus reuteri in autolytic models. RESULTS: A phosphate-induced autolytic model in de Man, Rogosa and Sharpe medium (MRS) was used. Viable cell counts were determined by plated on MRS-agar. Choline and hemicholinium-3 (HC-3) significantly blocked autolysis of L. reuteri at 360 mM and 4 mM, respectively. Viable cell counts corroborated these observations. Importantly, autolytically induced cells treated with choline and hemicholinium-3 were significantly more viable then even non-induced cells...
June 16, 2017: Biotechnology Letters
Bucheng Wang, Lu Jiang, Hechao Bai, Qiang Yong, Shiyuan Yu
OBJECTIVE: To protect the enzymes during fed-batch cellulase production by means of partial enzyme recovery at regular intervals. RESULTS: Extracellular enzymes were partially recovered at the intervals of 1, 2, or 3 days. Mycelia were also removed to avoid contamination. Increases in the total harvested cellulase (24-62%) and β-glucosidase (22-76%) were achieved. In fermentor cultivation when the enzymes were recovered every day with 15% culture broth. The total harvested cellulase and β-glucosidase activity increased by 43 and 58%, respectively, with fungal cell concentration maintained at 3...
June 13, 2017: Biotechnology Letters
Yejin Choi, Seong Yi Kwon, Ho Jung Oh, Sunbo Shim, Seokkee Chang, Hye Joo Chung, Do Keun Kim, Younsang Park, Younghee Lee
OBJECTIVES: The single radial immunodiffusion (SRID) assay, used to quantify hemagglutinin (HA) in influenza vaccines, requires reference reagents; however, because centralized production of reference reagents may slow the emergency deployment of vaccines, alternatives are needed. RESULTS: We investigated the production of HA proteins using recombinant DNA technology, rather than a traditional egg-based production process. The HA proteins were then used in an SRID assay as a reference antigen...
June 13, 2017: Biotechnology Letters
Chunqiu He, Wen Dong, Jing Li, Yanpeng Li, Chao Huang, Yanling Ma
OBJECTIVES: To improve rhamnolipid production and its potential application in removal of crude oil, the recombinant Pseudomonas aeruginosa strain DAB was constructed to enhance yield of rhamnolipids. RESULTS: Strain DAB had a higher yield of 17.3 g rhamnolipids l(-1) in the removal process with crude oil as the sole carbon source than 10 g rhamnolipids l(-1) of wild-type strain DN1, where 1% crude oil was degraded more than 95% after 14 days cultivation. These rhamnolipids reduced the surface tension of water from 72...
June 9, 2017: Biotechnology Letters
Ming-Zhu Wang, Zhuo-Lin Qiu, Xiang-Sheng Cai, Jing-Jing Li, Miao-Qin She, Yuan-Feng Xu, Ying-Song Wu
OBJECTIVE: To produce a recombinant spermatozoa antigen peptide using the E. coli: PhoA system on a protein chip for screening anti-sperm antibodies (ASA). RESULTS: The purity of the recombinant spermatozoa antigen exceeded 95% after two-step purification, as assessed using SDS-PAGE and HPLC. The diagnostic performance of a protein chip coated with the recombinant antigen peptide was evaluated by examining ASA in 51 infertile patients in comparison with a commercial ELISA kit...
June 9, 2017: Biotechnology Letters
Jia Liu, Linggai Cao, Yue Feng, Yuhua Li, Tao Li
OBJECTIVE: To suppress TNF-α-induced lipogenesis in sebocytes (associated with acne development) with microRNA-338-3p (miR-338-3p) and to explore the underlying mechanisms. RESULTS: TNF-α increased lipid droplet formation in sebocytes which were used as in vitro model of inflammation-induced acne. Flow cytometry and TLC assays validated that miR-338-3p could suppress TNF-α-induced lipid droplet formation, down-regulate the expression of PREX2a, and inactivate AKT signaling in sebocytes...
June 9, 2017: Biotechnology Letters
Tingting Zhang, Guangtao Ma, Yan Zhang, Hongda Huo, Yuqian Zhao
OBJECTIVE: To explore the molecular mechanism of microRNA-599(miR-599) in the migration and invasion of glioma. RESULT: Clinicopathological characteristics of 33 patients were analyzed. Based on reverse transcription-PCR, miR-599 was down-regulated in glioma tissues compared with adjacent normal brain tissues (P < 0.001). Moreover, negative correlations between miR-599 and periostin protein expression in glioma tissues (P < 0.01) and necrosis by magnetic resonance imaging (P < 0...
June 8, 2017: Biotechnology Letters
Maranda Esterhuizen-Londt, Stefanie Hertel, Stephan Pflugmacher
OBJECTIVES: To evaluate the remediation efficiency of Mucor hiemalis by comparing media elimination, uptake, and biotransformation of microcystin-LR with exposure to pure toxin versus a crude bloom extract. RESULTS: With exposure to the extract, the elimination rate of microcystin-LR from the media, which was 0.28 ng MC-LR l(-1) h(-1), was significantly higher compared to that achieved with exposure to the pure toxin (0.16 ng MC-LR l(-1) h(-1)) after 24 h...
June 8, 2017: Biotechnology Letters
Han Yong Park, Doo Hwan Kim, Iyyakkannu Sivanesan
The genus Ajuga L., belonging to Lamiaceae family, is widespread. The demand for Ajuga species has risen sharply because of their medicinal, ornamental, and pharmacological properties. These wide-ranging plants are being rapidly depleted due to over-collection for ornamental and medicinal purposes, as well as by habitat destruction and deforestation. Ajuga boninsimae, A. bracteosa, A. ciliate, A. genevensis, A. incisa, A. makinoi, A. multiflora, A. pyramidalis, A. shikotanensis, A. reptans, and A. vestita are categorized and protected as endangered plants...
June 8, 2017: Biotechnology Letters
Weiling Xu, Fangchao Gong, Ting Zhang, Baorong Chi, Jingyu Wang
OBJECTIVES: To investigate the roles of Dead end 1 (Dnd1) in modulating cancer stem cell-related traits of hepatocellular carcinoma (HCC). RESULTS: Dead end (Dnd1) inhibited spheroid formation, suppressed the expression of stemness-related genes, and increased sensitivity to sorafenib in HCC cells. Mechanistically, Dnd1 could bind to 3'-UTR of LATS2, the key kinase of Hippo pathway, thus elevating LATS2 mRNA stability and its expression, subsequently leading to phosphorylation of YAP and its cytoplasmic retention...
June 7, 2017: Biotechnology Letters
Xiaofan Yin, Libo Jiang, Jielai Yang, Lu Cao, Jian Dong
OBJECTIVES: To design and fabricate a 3D-printed cervical cage composite of polylactic acid (PLA)/nano-sized and β-tricalcium phosphate (β-TCP). RESULTS: CAD analysis provided a useful platform to design the preliminary cage. In vitro cell culture and in vivo animal results showed promising results in the biocompatibility of the constructs. Endplate matching evaluation showed better matching degree of 3D-printed cages than those of conventional cages. Biomechanical evaluation showed better mechanical properties of 3D-printed cages than those of conventional cages...
June 6, 2017: Biotechnology Letters
Xiaofeng Meng, Baozhong Shi
OBJECTIVES: To investigate the roles of miR-215 in high-grade glioma and to clarify the regulation of retinoblastoma 1 (RB1) by miR-215. RESULTS: miR-215 is frequently up-regulated in high-grade glioma tissues. Increased miR-215 expression is significantly associated with World Health Organization grade (P < 0.01) tumor size (P < 0.05) and poor prognosis (P < 0.01). Over-expression of miR-215 promoted cell proliferation and knockdown of miR-215 inhibited cell proliferation in vitro...
June 1, 2017: Biotechnology Letters
Ausra Peciulyte, Maria Pisano, Ronald P de Vries, Lisbeth Olsson
OBJECTIVES: To evaluate the potential of enzyme cocktails produced by five filamentous fungi to supplement the industrial cellulase cocktail, Celluclast 1.5L, in order to improve the efficiency of saccharification. RESULTS: The fungi were cultivated on wheat bran and the resulting supernatants were combined with Celluclast in enzymatic hydrolysis experiments to test their ability to hydrolyze wheat bran and five cellulose-rich substrates. The supernatant showing the best performance was that from an Aspergillus niger cellulase mutant...
June 1, 2017: Biotechnology Letters
Elaheh Sajadi, Valiollah Babaipour, Ali Asghar Deldar, Bagher Yakhchali, Seyed Safa-Ali Fatemi
OBJECTIVES: To evaluate the crystallinity index of the cellulose produced by Escherichia coli Nissle 1917 after heterologous expression of the cellulose synthase subunit D (bcsD) gene of Gluconacetobacter xylinus BPR2001. RESULTS: The bcsD gene of G. xylinus BPR2001 was expressed in E. coli and its protein product was visualized using SDS-PAGE. FTIR analysis showed that the crystallinity index of the cellulose produced by the recombinants was 0.84, which is 17% more than that of the wild type strain...
June 1, 2017: Biotechnology Letters
Xiufang Diao, Shuqing Sun
OBJECTIVE: To examine the role of miR-124a in LPS-induced septic cardiac insufficiency where underlying mechanism is unclear. RESULTS: Expression of miR-124a was decreased in myocardium of LPS-induced septic cardiac dysfunction model. miR-124a antagomiR or agomiR were injected via tail vein to induce miR-124a-dysregulated model. miR-124a antagomiR aggravated LPS-induced cardiac dysfunction and apoptosis, while miR-124a agomiR had the opposite effect. Syntaxin-2 (STX2) was indicated as a candidate target gene by bioinformatic software...
May 30, 2017: Biotechnology Letters
Chunxia Qiao, Xiaoling Lang, Longlong Luo, Shusheng Geng, Ming Lv, Jing Geng, Xinying Li, Jiannan Feng, Beifen Shen, Yan Li
OBJECTIVES: To find a "me-better" antibody by epitope-specific antibody optimization and multi-parametric analysis. RESULTS: Using epitope-specific library based on the commercial drug, Pertuzumab/2C4, we screened a novel human anti-HER2 antibody, MIL5, which has slightly higher affinity than the drug. MIL5 and 2C4 share the same epitope to bind HER2; however, MIL5 bound to HER2 His235-His245 more tightly than 2C4, which could be the main reason of its enhanced affinity...
May 30, 2017: Biotechnology Letters
Adnan Memic, Ali Navaei, Bahram Mirani, Julio Alvin Vacacela Cordova, Musab Aldhahri, Alireza Dolatshahi-Pirouz, Mohsen Akbari, Mehdi Nikkhah
There is a great need for the development of biomimetic human tissue models that allow elucidation of the pathophysiological conditions involved in disease initiation and progression. Conventional two-dimensional (2D) in vitro assays and animal models have been unable to fully recapitulate the critical characteristics of human physiology. Alternatively, three-dimensional (3D) tissue models are often developed in a low-throughput manner and lack crucial native-like architecture. The recent emergence of bioprinting technologies has enabled creating 3D tissue models that address the critical challenges of conventional in vitro assays through the development of custom bioinks and patient derived cells coupled with well-defined arrangements of biomaterials...
May 26, 2017: Biotechnology Letters
Tatsuya Kato, Kotaro Kikuta, Ayumi Kanematsu, Sachiko Kondo, Hirokazu Yagi, Koichi Kato, Enoch Y Park
OBJECTIVE: To synthesize complex type N-glycans in silkworms, shRNAs against the fused lobe from Bombyx mori (BmFDL), which codes N-acetylglucosaminidase (GlcNAcase) in the Golgi, was expressed by recombinant B. mori nucleopolyhedrovirus (BmNPV) in silkworm larvae. RESULTS: Expression was under the control of the actin promoter of B. mori or the U6-2 and i.e.-2 promoters from Orgyia pseudotsugata multiple nucleopolyhedrovirus (OpMNPV). The reduction of specific GlcNAcase activity was observed in Bm5 cells and silkworm larvae using the U6-2 promoter...
May 25, 2017: Biotechnology Letters
Yan Ru Wu, Yun Gyeong Kang, Ji Won Shin, Mi Jin Kim, Jung-Woog Shin
OBJECTIVE: To control the oscillatory behavior of the intracellular calcium ([Ca(2+)]i) concentration in endothelial cells via mechanical factors (i.e., various hydrostatic pressures) because [Ca(2+)]i in these cells is affected by blood pressure. RESULTS: Quantitative analyses based on real-time imaging showed that [Ca(2+)]i oscillation frequency and relative concentration increased significantly when 200 mm Hg pressure, mimicking hypertension, was applied for >10 min...
May 24, 2017: Biotechnology Letters
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