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Biotechnology Letters

Mark Schofield
There is an upsurge of interest in continuous bioprocessing, but currently continuous downstream bioprocessing has not been implemented to generate clinical material. This review focusses on the current state of the art of continuous downstream processing, highlighting the key advantages over traditional batch manufacturing. This allows the identification of scenarios where continuous downstream processing may be critical for commercial manufacturing success.
July 13, 2018: Biotechnology Letters
Jinxiang Zhu, Qiaoyun Zhu, Ruiqing Gong, Qin Xu, Menghao Cai, Tianyi Jiang, Xiangshan Zhou, Mian Zhou, Yuanxing Zhang
OBJECTIVE: Around one-fourth of the Komagataella phaffii genes encode hypothetical proteins with unknown functions. However, lack of powerful tools for genetic screening in K. phaffii significantly limits the functional analysis of these unknown genes. Transposon mutagenesis has been utilized as an insertional mutagenesis tool in many other organisms and would be extremely valuable if it could be applied in K. phaffii. RESULTS: In this study, we investigated in K...
July 12, 2018: Biotechnology Letters
Salvatore La China, Gabriele Zanichelli, Luciana De Vero, Maria Gullo
Acetic acid bacteria are versatile organisms converting a number of carbon sources into biomolecules of industrial interest. Such properties, together with the need to limit chemical syntheses in favor of more sustainable biological processes, make acetic acid bacteria appropriate organisms for food, chemical, medical, pharmaceutical and engineering applications. At current, well-established bioprocesses by acetic acid bacteria are those derived from the oxidative pathways that lead to organic acids, ketones and sugar derivates...
July 10, 2018: Biotechnology Letters
Yun Xue, Mengya Wang, Pengchao Zhao, Chunshan Quan, Xin Li, Lina Wang, Weina Gao, Jinghua Li, Xiangyang Zu, Dongliao Fu, Shuxiao Feng, Ping Li
Gram-negative bacilli such as Pseudomonas spp., Pseudoalteromonas sp., Angiococcus sp., Archangium sp., Burkholderia spp., Chromobacterium sp., Chondromyces sp., Cystobacter sp., Jahnella sp., Janthinobacterium sp., Lysobacter spp., Paraliomyxa sp., Photobacterium spp., Photorhabdus sp., Pontibacter sp., Ruegeria sp., Serratia sp., Sorangium sp., Sphingomonas sp., and Xenorhabdus spp. produce an enormous array of short peptides of 30 residues or fewer that are potential pharmaceutical drugs and/or biocontrol agents...
July 2, 2018: Biotechnology Letters
Yixing Zhang, Makoto Yoshida, Praveen V Vadlani
D-lactic acid is a versatile and important industrial chemical that can be applied in the synthesis of thermal-resistant poly-lactic acid. Biosynthesis of D-lactic acid can be achieved by a variety of microorganisms, including lactic acid bacteria, yeast, and fungi; however, the final product yield, optical purity, and the utilization of both glucose and xylose are restricted. Consequently, engineered microbial systems are essential to attain high titer, productivity, and complete utilization of sugars. Herein, we critically evaluate the promising wild-type microorganisms, as well as genetically modified microorganisms to produce enantiomerically pure D-lactic acid, particularly from renewable lignocellulosic biomass...
June 28, 2018: Biotechnology Letters
Weirui Zhao, Huanru Ding, Sheng Hu, Jun Huang, Changjiang Lv, Jiaqi Mei, Zhihua Jin, Shanjing Yao, Lehe Mei
In the original publication of the article, the affiliations of authors Jun Huang, Changjiang Lv and Jiaqi Mei were misplaced. The correct information for author affiliations is provided in this correction.
June 21, 2018: Biotechnology Letters
Yun Kong, Yajun Qu, Shengjun Wang, Peng George Wang, Min Chen
OBJECTIVE: To heterologously produce the Shigella dysenteriae serotype 1 O-polysaccharide (O-PS, O-antigen) in Escherichia coli by transferring the minimum number of genes instead of the entire O-PS gene cluster. RESULTS: The three glycosyltransferase genes (rfbR, rfbQ and rfp) responsible for the formation of the O-repeat unit were introduced into E. coli K-12 W3110 to synthesize S. dysenteriae 1 O-PS. The specific O-antigen ladder type with different chain lengths of O-repeat units was observed in the recombinant E...
June 21, 2018: Biotechnology Letters
Yangyang Wang, Xinke Yan, Shengyuan Zeng, Ting Zhang, Fengjuan Cheng, Rongjuan Chen, Changzhu Duan
OBJECTIVES: To investigate the interaction of E3 ubiquitin ligase UHRF2 with p21 and the mechanism of UHRF2 in repairing DNA damage caused by hydroxyurea (HU) in HEK293 cells. RESULTS: Western blotting indicated that the overexpression of UHRF2 reduced the level of p21, particularly in HEK293 cells. Immunoprecipitation and immunofluorescence staining reveled that UHRF2 combined with p21 in the nucleus. In addition, UHRF2 degraded p21 through ubiquitination and shortened the half-life of p21...
June 19, 2018: Biotechnology Letters
Chen Wang, Jiaqi Wang, Min Chen, Li Fan, Liang Zhao, Wen-Song Tan
OBJECTIVE: To explore the influence of ultra-low carbon dioxide partial pressure (pCO2 ) on the monoclonal antibody (mAb) N-glycosylation profile in Chinese hamster ovary (CHO) cell culture. RESULTS: In fed-batch bioreactor cultures, lowering the pCO2 in the medium (< 25 mmHg) via increasing headspace aeration decreased the cell viability and mAb production in CHO cells. Additionally, mAb galactosylation under low pCO2 was approximately 27.45 ± 2.13%, noticeably higher than that observed under normal pCO2 (21...
June 19, 2018: Biotechnology Letters
Seyed Ziyae Aldin Samsam Shariat, Fatemeh Borzouee, Mohammad Reza Mofid, Jaleh Varshosaz
OBJECTIVES: The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS). METHODS: Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using glutaraldehyde as cross-linker. Kinetic properties and stability of free and immobilized LPO were investigated. RESULTS: LPO was purified 59...
June 18, 2018: Biotechnology Letters
Zhi Miao, Qian Li, Jian Zhao, Peng Wang, Lei Wang, Hong-Peng He, Nan Wang, Hao Zhou, Tong-Cun Zhang, Xue-Gang Luo
OBJECTIVES: To establish stable infliximab-expressing Chinese hamster ovary (CHO) cells with high tolerance to serum-free culture. RESULTS: Bcl-2 antagonist/killer 1 (BAK1), which is a key mediator of the apoptosis pathway, was disrupted, and infliximab, which is a broadly used monoclonal antibody for the treatment of rheumatoid arthritis and other autoimmune diseases, was incorporated into the BAK1 locus of the CHO chromosome using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas genome-editing technique...
June 18, 2018: Biotechnology Letters
Alessandra Stefan, Natalia Calonghi, Fabrizio Schipani, Fabrizio Dal Piaz, Giorgio Sartor, Alejandro Hochkoeppler
OBJECTIVE: We attempted to overexpress Human Histone Deacetylase 1 (HDAC1) in Escherichia coli. RESULTS: A synthetic gene coding for HDAC1, and optimised for E. coli codon usage, was cloned into pBADHisB, generating pBAD-rHDAC1. This construct was used to transform E. coli TOP10, and the target protein was overexpressed and partially purified. According to its elution volume from a Superdex 200 column, the partially purified rHDAC1 was obtained in aggregated form, i...
June 14, 2018: Biotechnology Letters
P Castellano, M Pérez Ibarreche, L Longo Borges, F C Niño Arias, G R Ross, E C Pereira De Martinis
OBJECTIVES: The objective of this study was to evaluate the ability of Lactobacillus curvatus CRL705, CRL1532, and CRL1533 and Lactobacillus sakei CRL1613 to survive under simulated gastrointestinal conditions. Moreover, a microencapsulation approach was proposed to improve gastrointestinal survival. Finally, experiments were performed to demonstrate that Lactobacillus spp. can modulate the ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells. RESULTS: Lactobacillus strains were encapsulated in alginate beads to enhance the survival of bacteria under in vitro gastrointestinal conditions...
June 8, 2018: Biotechnology Letters
Xiaoyu Guo, Zhimin Yu, Meihui Zhang, Wenzhu Tang, Yumei Sun, Xianzhen Li
OBJECTIVE: To enhance the production of phenolic compounds during barley germination using chitooligosaccharide as an elicitor to improve the antioxidant capacity of malt. RESULTS: When used as an elicitor for barley germination, chitooligosaccharide with a molecular weight of 3 kDa, added at 10 mg/kg barley kernels during the first steeping cycle, led to the maximum production of phenolic compounds. Compared with the control with no chitooligosaccharide added to the steeping water, the total phenolic content was increased by 54...
June 6, 2018: Biotechnology Letters
Jessica Thomas, Desiree Jones, Leni Moldovan, Mirela Anghelina, Keith J Gooch, Nicanor I Moldovan
OBJECTIVES: Attachment of magnetic particles to cells is needed for a variety of applications but is not always possible or efficient. Simpler and more convenient methods are thus desirable. In this study, we tested the hypothesis that endothelial cells (EC) can be loaded with micron-size magnetic beads by the phagocytosis-like mechanism 'angiophagy'. To this end, human umbilical vein EC (HUVEC) were incubated with magnetic beads conjugated or not (control) with an anti-VEGF receptor 2 antibody, either in suspension, or in culture followed by re-suspension using trypsinization...
June 6, 2018: Biotechnology Letters
Samiya Khan, Sanjay Gupta, Nidhi Gupta
OBJECTIVES: To improve the quality of diesel fuel via removal of aromatic compounds using Pseudomonas sp. RESULTS: In the present study Pseudomonas sp. was able to remove 94% of fluorene, 59% of phenanthrene, 49% of anthracene, 52% of fluoranthene, 45% of pyrene and 75% carbazole present in diesel oil. Additionally, it also does not affect the aliphatic content of fuel thus maintaining the carbon backbone of the fuel. CONCLUSIONS: Pseudomonas sp...
May 25, 2018: Biotechnology Letters
Dong Wei, Weili Yu, Chunyang Hu, Lijuan Shen, Tao Hu
OBJECTIVE: Capsular polysaccharide (PS) of Streptococcus pneumoniae is a key virulence factor and typically conjugated with a carrier protein. It is necessary to improve the immunogenicity of the conjugate vaccine against S. pneumoniae. RESULTS: A phenyl linker between tetanus toxoid (TT) and S. pneumoniae Type 14 PS was used to improve the PS-specific immunogenicity of the conjugate vaccine. As compared with the one with the amyl linker (PS-TT), the conjugate with the phenyl linker (PS-phe-TT) decreased the TT-specific IgG titers and significantly increased the PS-specific IgG titers and the IL-5 level...
May 25, 2018: Biotechnology Letters
Margarita Stritzler, Ana Diez Tissera, Gabriela Soto, Nicolás Ayub
OBJECTIVES: Identification of novel microbial factors contributing to plant protection against abiotic stress. RESULTS: The genome of plant growth-promoting bacterium Pseudomonas fluorescens FR1 contains a short mobile element encoding a novel type of extracellular polyhydroxybutyrate (PHB) polymerase (PhbC) associated with a type I secretion system. Genetic analysis using a phbC mutant strain and plants showed that this novel extracellular enzyme is related to the PHB production in planta and suggests that PHB could be a beneficial microbial compound synthesized during plant adaptation to cold stress...
May 24, 2018: Biotechnology Letters
Zhen-Hai Li, Min Liu, Feng-Qing Wang, Dong-Zhi Wei
OBJECTIVES: To test the applicability of Cpf1 from Francisella novivida in genomic integration of in vivo assembled DNA parts in Saccharomyces cerevisiae. RESULTS: An easy-to-use vector toolkit, containing a CEN6/ARS4 plasmid expressing Cpf1 from Francisella novivida (FnCpf1) and a 2 μ plasmid for crRNA or crRNA array expressing, was constructed for Cpf1-assisted genomic integration in S. cerevisiae. Our results showed that FnCpf1 allowed for targeted singleplex, doubleplex, and tripleplex genomic integration of in vivo assembled DNA parts with efficiencies of 95, 52, and 43%, respectively...
May 24, 2018: Biotechnology Letters
Hoda Sabati, Hossein Motamedi
OBJECTIVE: Water in oil emulsions increase oil processing costs and cause damage to refinery equipment which necessitates demulsification. Since chemical demulsifiers cause environmental pollution, biodemulsifiers have been paid more attention. This study aims to identify biodemulsifier-producing bacteria from petroleum contaminated environments. RESULTS: As a result, several biodemulsifier producing strains were found that Stenotrophomonas sp. strain HS7 (accession number: MF445088) which produced a cell associated biodemulsifier showed the highest demulsifying ratio, 98...
May 15, 2018: Biotechnology Letters
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