Huaina Qiu, Ruohan Wang, Junmin Xing, Lu Li, Zhiyan Gao, Jiajie Li, Chao Fang, Feihu Shi, Feng Mo, Lin Liu, Yi Zhao, Hongyan Xie, Shan Zhao, Jun Huang
Interleukin 9 (IL-9) is an effective cytokine secreted by newly defined Th9 cells, which is involved in allergic and infectious diseases. In this study, lymphocytes were isolated from mesenteric lymph node (MLN), spleen, liver, lung, and Peyer's patches (PP) of C57BL/6 mice 5-6 weeks after S. japonicum infection, intracellular cytokine staining was done to detect the percentage of IL-9-producing CD4+ T cells. The qPCR and ELISA were used to verify the content of IL-9 in MLN. The population of IL-9-producing lymphocyte subset was identified by FACS...
April 20, 2023: Molecular and Biochemical Parasitology