journal
MENU ▼
Read by QxMD icon Read
search

Molecular and Biochemical Parasitology

journal
https://www.readbyqxmd.com/read/29175581/application-of-shape-reveals-in-vivo-rna-folding-under-normal-and-growth-stressed-conditions-in-the-human-parasite-entamoeba-histolytica
#1
Ashwini Kumar Ray, Sarah Naiyer, Shashi Shekhar Singh, Alok Bhattacharya, Sudha Bhattacharya
Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) is a versatile sequence independent method to probe RNA structure in vivo and in vitro. It has so far been tried mainly with model organisms. We show that cells of Entamoeba histolytica, a protozoan parasite of humans are hyper-sensitive to the in vivo SHAPE reagent, NAI, and show rapid loss of viability and RNA integrity. We optimized treatment conditions with 5.8S rRNA and Eh_U3 snoRNA to obtain NAI-modification while retaining RNA integrity...
November 22, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29169803/expression-purification-and-characterization-of-two-leucine-aminopeptidases-of-the-blood-fluke-schistosoma-mansoni
#2
Gabriela Maggioli, Gabriel Rinaldi, Ines Giaudrone, Patricia Berasain, José F Tort, Paul J Brindley, Carlos Carmona
Schistosomiasis is a major neglected tropical disease (NTD) and considered the most important of the human helminthiases in terms of morbidity and mortality. Whereas treatment with praziquantel has been effective since the 1980s, the potential for the emergence of drug resistance has propelled the search for new interventions. Studies have revealed key roles of proteases in parasitic helminths during establishment of infection, tissue invasion, immune evasion, parasite feeding and development throughout the different developmental stages, pinpointing them as possible candidates...
November 20, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29157661/analysis-of-codon-usage-pattern-of-mitochondrial-protein-coding-genes-in-different-hookworms
#3
Bornali Deb, Arif Uddin, Gulshana Akthar Mazumder, Supriyo Chakraborty
The phenomenon of unequal usage of synonymous codons encoding an amino acid in which some codons are more preferred to others is the codon usage bias (CUB) and it is species specific. Analysis of CUB helps in understanding evolution at molecular level and acquires significance in mRNA translation, design of transgenes and new gene discovery. In our current study, we analyzed synonymous codon usage pattern and the factors influencing it on mitochondrial protein coding genes of 6 different hookworms i.e. Ancylostoma ceylanicum, Ancylostoma duodenale, Necator americanus, Ancylostoma tubaeforme, Ancylostoma caninum and Uncinaria sanguinis as no work was reported yet...
November 17, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29155083/tbvps15-is-required-for-vesicular-transport-and-cytokinesis-in-trypanosoma-brucei
#4
Alejandra C Schoijet, Kildare Miranda, Tamara Sternlieb, Nadia M Barrera, Wendell Girard-Dias, Wanderley de Souza, Guillermo D Alonso
The class III phosphatidylinositol 3-kinase (PI3K) Vps34 is an important regulator of key cellular functions, including cell growth, survival, intracellular trafficking, autophagy and nutrient sensing. In yeast, Vps34 is associated with the putative serine/threonine protein kinase Vps15, however, its role in signaling has not been deeply evaluated. Here, we have identified the Vps15 orthologue in Trypanosoma brucei, named TbVps15. Knockdown of TbVps15 expression by interference RNA resulted in inhibition of cell growth and blockage of cytokinesis...
November 16, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29133150/structure-and-function-of-per-arnt-sim-domains-and-their-possible-role-in-the-life-cycle-biology-of-trypanosoma-cruzi
#5
REVIEW
Maura Rojas-Pirela, Daniel J Rigden, Paul A Michels, Ana J Cáceres, Juan Luis Concepción, Wilfredo Quiñones
Per-ARNT-Sim (PAS) domains of proteins play important roles as modules for signalling and cellular regulation processes in widely diverse organisms such as Archaea, Bacteria, protists, plants, yeasts, insects and vertebrates. These domains are present in many proteins where they are used as sensors of stimuli and modules for protein interactions. Characteristically, they can bind a broad spectrum of molecules. Such binding causes the domain to trigger a specific cellular response or to make the protein containing the domain susceptible to responding to additional physical or chemical signals...
November 10, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29128656/molecular-and-functional-characterization-of-two-malic-enzymes-from-leishmania-parasites
#6
Lucila Giordana, Máximo Hernán Sosa, Alejandro E Leroux, Elkin F Rodas Mendoza, Patricia Petray, Cristina Nowicki
Leishmania parasites cause a broad spectrum of clinical manifestations in humans and the available clinical treatments are far from satisfactory. Since these pathogens require large amounts of NADPH to maintain intracellular redox homeostasis, oxidoreductases that catalyze the production of NADPH are considered as potential drug targets against these diseases. In the sequenced genomes of most Leishmania spp. two putative malic enzymes (MEs) with an identity of about 55% have been identified. In this work, the ME from L...
November 8, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29107734/involvement-of-snare-protein-ykt6-in-glycosome-biogenesis-in-trypanosoma-brucei
#7
Hiren Banerjee, Richard A Rachubinski
The kinetoplastid parasites Trypanosoma and Leishmania are etiologic agents of diseases like African sleeping sickness, Chagas and leishmaniasis that inflict many tropical and subtropical parts of the world. These parasites are distinctive in that they compartmentalize most of the usually cytosolic enzymes of the glycolytic pathway within a peroxisome-like organelle called the glycosome. Functional glycosomes are essential in both the procyclic and bloodstream forms of trypanosomatid parasites, and mislocalization of glycosomal enzymes to the cytosol is fatal for the parasite...
December 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29037797/flow-cytometric-characterization-of-encystation-in-entamoeba-invadens
#8
Brenda H Welter, Michael G Sehorn, Lesly A Temesvari
Entamoeba histolytica causes dysentery and liver abscess mostly in countries that lack proper sanitation. Infection is acquired by ingestion of the cyst form in contaminated food or water. E. histolytica does not encyst in vitro; thus, E. invadens, a reptilian parasite that encysts in vitro, has been used as a surrogate. Cysts are small and possess chitin-rich walls. These are characteristics that may be exploited by flow cytometry. We stained encysting E. invadens cells with a fluorescent chitin stain, and analyzed fluorescence and forward scatter by flow cytometry...
December 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28988930/comparison-of-two-methods-for-transformation-of-plasmodium-knowlesi-direct-schizont-electroporation-and-spontaneous-plasmid-uptake-from-plasmid-loaded-red-blood-cells
#9
Roberto R Moraes Barros, Tyler J Gibson, Whitney A Kite, Juliana M Sá, Thomas E Wellems
Human infections from Plasmodium knowlesi present challenges to malaria control in Southeast Asia. P. knowlesi also offers a model for other human malaria species including Plasmodium vivax. P. knowlesi parasites can be cultivated in the laboratory, and their transformation is standardly performed by direct electroporation of schizont-infected red blood cells (RBCs) with plasmid DNA. Here we show that the efficiency of direct electroporation is exquisitely dependent on developmental age of the schizonts. Additionally, we show that transformation of P...
December 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28965880/the-divergent-n-terminal-domain-of-tim17-is-critical-for-its-assembly-in-the-tim-complex-in-trypanosoma-brucei
#10
Ebony Weems, Ujjal K Singha, Joseph T Smith, Minu Chaudhuri
Trypanosoma brucei Tim17(TbTim17), the single member of the Tim17/23/22 protein family, is an essential component of the translocase of the mitochondrial inner membrane (TIM). In spite of the conserved secondary structure, the primary sequence of TbTim17, particularly the N-terminal hydrophilic region, is significantly divergent. In order to understand the function of this region we expressed two N-terminal deletion mutants (Δ20 and Δ30) of TbTim17 in T. brucei. Both of these mutants of TbTim17 were targeted to mitochondria, however, they failed to complement the growth defect of TbTim17 RNAi cells...
December 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28843781/biochemical-composition-of-marine-monogenean-parasite-eggs
#11
Alexander K Brazenor, David S Francis, Kate S Hutson, Alexander G Carton
This study on the eggs of the tropical monogenean Neobenedenia girellae presents the first detailed quantitative biochemical information of a marine parasite species' eggs. Moisture and protein composed the majority of the contents of freshly laid eggs (79.12±0.82 and 11.51±0.49% respectively) followed by lipid (2.50±0.15%). Lipids were composed of approximately equal amounts of saturated, monounsaturated, and polyunsaturated fatty acids and the predominant lipid class was triacylglycerol (33.82±1.20%)...
December 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28887063/the-50kda-metalloproteinase-tvmp50-is-a-zinc-mediated-trichomonas-vaginalis-virulence-factor
#12
Jonathan Puente-Rivera, José Luis Villalpando, Alma Villalobos-Osnaya, Laura Isabel Vázquez-Carrillo, Gloria León-Ávila, María Dolores Ponce-Regalado, César López-Camarillo, Jose Miguel Elizalde-Contreras, Eliel Ruiz-May, Rossana Arroyo, María Elizbeth Alvarez-Sánchez
Trichomonas vaginalis is a protozoan parasite that can adapt to the trichomonicidal Zn(2+) concentrations of the male urogenital tract microenvironment. This adaptation is mediated by molecular mechanisms, including proteinase expression, that are regulated by cations such as Zn(2+). Herein, we characterized the previously identified 50kDa metalloproteinase aminopeptidase P (M24 family) member TvMP50 as a new Zn(2+)-mediated parasite virulence factor. Quantitative RT-PCR and indirect immunofluorescence assays corroborated the positive regulation of both mp50 gene expression and native TvMP50 protein overexpression in the cytoplasm and secretion products of parasites grown in the presence of Zn(2+)...
October 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28847609/structural-and-evolutionary-analysis-of-leishmania-alba-proteins
#13
Kauê Santana da Costa, João Marcos Pereira Galúcio, Elvis Santos Leonardo, Guelber Cardoso, Élcio Leal, Guilherme Conde, Jerônimo Lameira
The Alba superfamily proteins share a common RNA-binding domain. These proteins participate in a variety of regulatory pathways by controlling developmental gene expression. They also interact with ribosomal subunits, translation factors, and other RNA-binding proteins. The Leishmania infantum genome encodes two Alba-domain proteins, LiAlba1 and LiAlba3. In this work, we used homology modeling, protein-protein docking, and molecular dynamics (MD) simulations to explore the details of the Alba1-Alba3-RNA complex from Leishmania infantum at the molecular level...
October 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28844893/tandem-affinity-purification-of-exosome-and-replication-factor-c-complexes-from-the-non-human-infectious-kinetoplastid-parasite-crithidia-fasciculata
#14
Wakisa Kipandula, Terry K Smith, Stuart A MacNeill
Kinetoplastid parasites are responsible for a range of diseases with significant global impact. Trypanosoma brucei and Trypanosoma cruzi cause human African trypanosomiasis and Chagas disease, respectively, while various Leishmania species are responsible for cutaneous, mucocutaneous and visceral leishmaniasis. Understanding the biology of these organisms is key for effective diagnosis, prophylaxis and treatment. The insect parasite Crithidia fasciculata offers a safe and low-cost alternative for studies of kinetoplastid biology...
October 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28843782/rodent-free-cyclical-transmission-of-trypanosoma-brucei-brucei
#15
Arunasalam Naguleswaran, Isabel Roditi
We provide a simple protocol enabling cyclical transmission of Trypanosoma brucei brucei to be performed without the need for mammals. These procedures have two advantages: they are in line with 3R principles of animal use - replace, refine, reduce - and may enable more laboratories to study the complete life cycle.
October 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28818675/presence-of-quintuple-dhfr-n51-c59-s108-dhps-a437-k540-mutations-in-plasmodium-falciparum-isolates-from-pregnant-women-and-the-general-population-in-nanoro-burkina-faso
#16
Esmée Ruizendaal, Marc C Tahita, Maminata Traoré-Coulibaly, Halidou Tinto, Henk D F H Schallig, Petra F Mens
Sulphadoxine-pyrimethamine (SP) is only used for intermittent preventive treatment during pregnancy (IPTp) in most Sub-Saharan African countries. However, there are concerns about the efficacy of IPTp-SP because of increasing resistance. Combinations of point mutations in the dhps and dhfr genes of Plasmodium falciparum are associated with SP resistance, in particular the quintuple dhfr (N51, C59, S108) - dhps (codons A437, K540) mutant. In Nanoro, Burkina Faso, filter paper samples from pregnant women at first antenatal care visit and at delivery plus samples from the general population (GP) were studied for dhfr and dhps mutations by sequencing...
October 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28811124/two-cap-residues-in-the-s1-subsite-of-a-plasmodium-falciparum-m1-family-aminopeptidase-promote-broad-specificity-and-enhance-catalysis
#17
Matthew Rosati, Seema Dalal, Michael Klemba
The aminopeptidase PfA-M1 is a key contributor to peptide catabolism in the human malaria parasite Plasmodium falciparum. PfA-M1 substrate specificity is shaped by the cylindrical S1 subsite, which accommodates the sidechain of the substrate P1 residue. At the top of the S1 subsite are two "cap" residues, E572 and M1034, that are positioned to influence S1 subsite specificity. In this study, we have mutated the cap residues, individually and together, and have evaluated the effects on PfA-M1 specificity and catalytic efficiency...
October 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28803842/experimental-evidence-for-hybridization-of-closely-related-lineages-in-plasmodium-relictum
#18
Vaidas Palinauskas, Rasa Bernotienė, Rita Žiegytė, Staffan Bensch, Gediminas Valkiūnas
Over 50 avian Plasmodium species have been described. However, PCR-based information shows much broader diversity of genetic lineages in these parasites. This discrepancy indicates insufficient knowledge about taxonomic diversity and boundaries of a single species in avian Plasmodium species. In recent taxonomy, most of genetically closely related lineages that share the same morphology and development patterns are attributed to the same biological species, but there is no information if these lineages are able to cross...
October 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28716719/metacyclic-vsg-expression-site-promoters-are-recognized-by-the-same-general-transcription-factor-that-is-required-for-rna-polymerase-i-transcription-of-bloodstream-expression-sites
#19
Nikolay G Kolev, Arthur Günzl, Christian Tschudi
Infectious metacyclic Trypanosoma brucei cells develop in the salivary glands of tsetse flies. A critical aspect of the developmental program leading to acquisition of infectivity is the synthesis of a variant surface glycoprotein (VSG) coat. Metacyclic VSG genes are transcribed from a set of specialized VSG expression sites (ESs) that differ from bloodstream VSG ESs by being monocistronic, being significantly shorter, lacking long stretches of 70-bp repeats, and having distinct promoter sequences. Both metacyclic and bloodstream VSG ESs are transcribed by the multifunctional T...
September 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28729071/transient-transfection-of-intraerythrocytic-babesia-gibsoni-using-elongation-factor-1-alpha-promoter
#20
Mingming Liu, Masahito Asada, Shinuo Cao, Paul Franck Adjou Moumouni, Patrick Vudriko, Artemis Efstratiou, Hassan Hakimi, Tatsunori Masatani, Fujiko Sunaga, Shin-Ichiro Kawazu, Junya Yamagishi, Xuenan Xuan
The development of gene manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not yet been established for Babesia gibsoni. Here, we report for the first time, the successful transient transfection of B. gibsoni. The plasmid containing the firefly luciferase reporter gene (pBS-ELA) was transfected into B. gibsoni by an AMAXA 4D Nucleofector™ device. Transfection using program FA113 and Lonza buffer SF showed the highest luciferase expression...
July 17, 2017: Molecular and Biochemical Parasitology
journal
journal
27960
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"