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Journal of Virological Methods

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https://www.readbyqxmd.com/read/30003926/a-novel-magnetic-beads-based-method-for-polioviral-concentration-from-environmental-samples
#1
Yuri Perepliotchikov, Itai Benhar, Yossi Manor, Thomas Wilton, Manasi Majumdar, Javier Martin, Ella Mendelson, Lester M Shulman
Environmental surveillance (EnvS)2 is an important tool for monitoring the presence of poliovirus in endemic and poliovirus free regions. Unlike acute flaccid paralysis (AFP)3 surveillance, EnvS can monitor large populations using small numbers of samples and detect the introduction of poliovirus even before the appearance of AFP cases. Early detection and timely response can prevent the onset of poliovirus associated AFP, as was demonstrated by silent poliovirus transmission in Israel in 2013. Although EnvS is currently recommended as supplementary to AFP surveillance, it is limited to laboratories with equipment for poliovirus concentration and to regions where samples can be easily transported under temperature controlled conditions to such facilities...
July 9, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/30003925/an-optimized-high-throughput-fluorescence-plate-reader-based-rsv-neutralization-assay
#2
Yong-Peng Sun, Wei Zhang, Qin-Jian Zhao, Jian-Li Cao, Lu-Jing Zhang, Jiang-Yan Xiang, Jun-Yu Si, Xue Lin, Li Chen, Zi-Zheng Zheng, Ning-Shao Xia
A licensed vaccine for respiratory syncytial virus (RSV) has yet to be developed, and a reliable and repeatable neutralizing assay is indispensable for vaccine development. Here, we demonstrated an optimized high-throughput RSV neutralization assay that utilizes a fluorescence plate reader (reader) as a substitute for flow cytometry to detect fluorescent signals in RSV-A2 mKate-infected cells. Furthermore, this study tested the influence of virus input and infectivity on the neutralizing assay and highlighted critical factors (together with a suggested protocol) for obtaining stable data using this assay...
July 9, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29981297/comparison-of-viral-elution-concentration-methods-for-recovering-noroviruses-from-deli-meats
#3
Isabelle da Silva Luz, Marize Pereira Miagostovich
This study aimed to assess viral elution-concentration methods for recovering noroviruses from deli meats. Spiking experiments were conducted to evaluate the recovery success rates and recovery efficiencies of human norovirus (NoV) GI and GII and murine norovirus 1 (MNV-1) using polyethylene glycol (PEG 6000) precipitation, skimmed milk flocculation (SMF), TRIzol® reagent, and a combination of PEG/TRIzol® and SMF/TRIzol® methods. Molecular analysis using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) revealed TRIzol® as the best method to be used for viral recovery from ham with medium recovery rates of 37...
July 4, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29981296/identification-and-genetic-characterization-of-bovine-enterovirus-by-combination-of-two-next-generation-sequencing-platforms
#4
Maria Serena Beato, Maurilia Marcacci, Eliana Schiavon, Luciano Bertocchi, Marco Di Domenico, Alessia Peserico, Monica Mion, Guendalina Zaccaria, Lara Cavicchio, Iolanda Mangone, Elena Soranzo, Claudio Patavino, Cesare Cammà, Alessio Lorusso
Prompt and accurate diagnosis is warranted for infectious diseases of domestic animals which may have a significant impact on animal production or clinical practice. In this study, the identification and genetic characterization of a bovine enterovirus (BEV) strain isolated from a calf with diarrhea, are described. Two different next generation sequencing platforms were employed. Shotgun metagenomic accomplished by MinION sequencing (Oxford Nanopore Technologies) allowed the identification of BEV RNA from a cell-culture isolate...
July 4, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29969601/analysis-of-drug-resistance-mutations-in-whole-blood-dna-from-hiv-1-infected-patients-by-single-genome-and-ultradeep-sequencing-analysis
#5
Alma Sotillo, Olalla Sierra, Lorena Martínez-Prats, Francisca Gutiérrez, Sagrario Zurita, Federico Pulido, Rafael Rubio, Rafael Delgado
In HIV-1 infected patients blood CD4+ T lymphocytes could be a valuable target to analyze drug resistance mutations (DRM) selected over the course of the infection. However, detection of viral resistance mutations in cellular DNA by standard genotype resistance techniques (SGRT) is suboptimal. Whole blood DNA (wbDNA) from 12 HIV-1 infected patients on ART was studied by Single Genome Sequencing (SGS) and 8 of them also by Ultradeep pyrosequencing (UDP). Results were compared with contemporary and historical DRM detected in plasma by SGRT during follow up...
June 30, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29966597/titration-of-cell-associated-varicella-zoster-virus-with-the-mv9g-reporter-cell-line-for-antiviral-studies
#6
Xiaojie Li, Xiaoxia Li, Wei Gong, Guanqing Wang, Zhenling Lu, Ningjun Wu, Chengxiang Lian, Ling Huang, Naoki Inoue
Titration of the cell-associated virus (CAV) of varicella-zoster virus (VZV) is essential for antiviral studies. A VZV reporter cell line, MV9G, generated in our previous study expresses firefly luciferase upon CAV infection in a dose-dependent manner, suggesting that use of the cell line for titration is feasible. In this study, MeWo cells infected with VZV vaccine Oka (vOka) strain or with clinical isolates obtained from patients with varicella or zoster were used as CAV. A co-culture of MV9G cells with the virus-infected MeWo cells were set up and optimized for titration of CAV...
June 29, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29964077/an-improved-reverse-transcription-loop-mediated-isothermal-amplification-assay-for-sensitive-and-specific-detection-of-serotype-o-foot-and-mouth-disease-virus
#7
Da-Rae Lim, Hye-Ryung Kim, Min-Ji Park, Ha-Gyeong Chae, Bok-Kyung Ku, Jin-Ju Nah, So-Yoon Ryoo, Sung-Hwan Wee, Yu-Ri Park, Hyo-Sung Jeon, Ji-Jeong Kim, Bo-Young Jeon, Hyeong-Woo Lee, Sang-Geon Yeo, Choi-Kyu Park
A sensitive and specific swarm primer-based reverse transcription loop-mediated isothermal amplification (sRT-LAMP) assay for the detection of serotype O foot-and-mouth disease virus (FMDV) was developed and evaluated. The assay specifically amplified the VP3 gene of serotype O FMDV, but did not amplify the VP3 gene of other serotype FMDVs or any other viruses. The limit of detection of the assay was 102 TCID50 /mL or 103 RNA copies/µL, which is 100 times lower than that of the RT-LAMP assay without swarm primers...
June 28, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29964076/multi-laboratory-comparison-of-three-commercially-available-zika-igm-enzyme-linked-immunosorbent-assays
#8
Alison Jane Basile, Christin Goodman, Kalanthe Horiuchi, Angela Sloan, Barbara W Johnson, Olga Kosoy, Janeen Laven, Amanda J Panella, Isabel Sheets, Freddy Medina, Emelissa J Mendoza, Monica Epperson, Panagiotis Maniatis, Vera Semenova, Evelene Steward-Clark, Emily Wong, Brad J Biggerstaff, Robert Lanciotti, Michael Drebot, David Safronetz, Jarad Schiffer
No abstract text is available yet for this article.
June 28, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29958922/a-monoclonal-antibody-to-diii-e-protein-allowing-the-differentiation-of-west-nile-virus-from-other-flaviviruses-by-a-lateral-flow-assay
#9
Belén Rebollo, Teresa Pérez, Ana Camuñas, Elisa Pérez-Ramírez, Francisco Llorente, Maria Paz Sanchez-Seco, Miguel Ángel Jiménez-Clavero, Ángel Venteo
No abstract text is available yet for this article.
June 26, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29958921/development-of-a-droplet-digital-rt-pcr-for-the-quantification-of-foot-and-mouth-virus-rna
#10
Tatiana F Pinheiro-de-Oliveira, Antonio A Fonseca, Marcelo F Camargos, Mateus Laguardia-Nascimento, Anapolino M de Oliveira, Ana C P Cottorello, Aristoteles Goes-Neto, Edel F Barbosa-Stancioli
Foot-and-mouth-disease (FMD) is a highly contagious disease of domestic animals which can result in substantial economic losses, caused by the FMD virus (FMDV). The aim of this study was to develop and standardize a novel reverse transcriptase droplet digital PCR (RT-ddPCR) assay for the quantification of FMDV RNA. This assay was based upon an OIE-recognized real-time RT-PCR that detects the 3D-encoding region of FMDV. The limit of detection at 101.4 TCID50 /mL and 26.5 copies was determined using FMDV-A24-Cruzeiro-virus and a plasmid containing the 3D-FMDV sequences, respectively...
June 26, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29949737/an-efficient-recovery-method-for-enteric-viral-particles-from-agricultural-soils
#11
Julie Brassard, Marie-Josée Gagné
Enteric viruses have been recognized as the leading cause of non-bacterial gastroenteritis and hepatitis outbreaks around the world. Understanding their prevalence and persistence in the environment is important for the effective control of these infections. The aim of this study was to develop an efficient recovery procedure for viral infectious particles from agricultural soils. Samples (25 g) of soil (black earth soil, loamy soil, and sandy soil) were spiked with murine norovirus (MNV) and feline calicivirus (FCV), mixed with five different buffers and viral genetic material was extracted by 3 commercial kits...
June 24, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29949736/clover-tagged-porcine-reproductive-and-respiratory-syndrome-virus-infectious-clones-for-rapid-detection-of-virus-neutralizing-antibodies
#12
Baicheng Huang, Xia Xiao, Biyun Xue, En-Min Zhou
Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is a widespread disease that affects domestic pigs of all ages. Accurate and rapid detection of PRRSV specific neutralizing antibodies levels in a pig herd is beneficial for the evaluation of the herd's immunity to combat the specific viral infection. However, the current methods for viral detection, including fluorescent focus neutralization (FFN) and cytopathic effect (CPE) reduction neutralizing assays, are subjective and time-consuming...
June 24, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29944907/detection-of-tobamoviruses-by-rt-pcr-using-a-novel-pair-of-degenerate-primers
#13
Yueyue Li, Guanlin Tan, Pingxiu Lan, Ansheng Zhang, Yong Liu, Ruhui Li, Fan Li
A generic RT-PCR assay was developed for the universal detection of viruses of the genus Tobamovirus using a novel pair of degenerate primers designed based on conserved regions on replicase genes of 32 tobamoviruses. The assay detected nine tobamoviruses, including six Solanaceae-infecting subgroup tobamoviruses of Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV), Tomato mottle mosaic virus (ToMMV), Tobacco mottle green mosaic virus (TMGMV), Pepper mild mottle virus (PMMoV), Paprika mild mottle virus (PaMMV), one Orchidaceae-infecting tobamovirus of Odontoglossum ringspot virus (ORSV) and two Cucurbitaceae-infecting subgroup tobamoviruses of Cucumber green mottle mosaic virus (CGMMV) and Zucchini green mottle mosaic virus (ZGMMV), with high amplification efficiency, specificity and sensitivity...
June 23, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29940196/viral-interference-between-low-pathogenic-avian-influenza-h9n2-and-avian-infectious-bronchitis-viruses-in-vitro-and-in-ovo
#14
Aouini Rim, Laamiri Nacira, Ghram Abdeljelil
BACKGROUND: Low pathogenic avian influenza (LPAI) H9N2 and infectious bronchitis virus (IBV) are important pathogens of poultry, causing important economic losses for the sector. Replication interference between these two viruses was described using cell cultures (CC) and embryonated chicken eggs (ECE). Chicken embryo lung (CEL) and ECE were simultaneously or sequentially infected with IBV vaccine strain (H120) and LPAIV-H9N2 (A/Ck/TUN/145/2012) to evaluate viral interactionsin vitro and in ovo, respectively...
June 22, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29936095/development-of-a-real-time-reverse-transcription-loop-mediated-isothermal-amplification-method-rt-lamp-for-detection-of-a-novel-swine-acute-diarrhea-syndrome-coronavirus-sads-cov
#15
Huanan Wang, Feng Cong, Fanwen Zeng, Yuexiao Lian, Xiangnan Liu, Manlin Luo, Pengju Guo, Jingyun Ma
A novel swine acute diarrhea syndrome Coronavirus (SADS-CoV) that causes severe diarrhea in suckling piglets was identified in southern China in 2017. A simple and rapid detection test was developed for this virus using real-time RT-LAMP based on the conserved N gene of the virus. The method had a detection limit of 1.0 × 101 copies/µL with no cross-reactions with classical swine fever virus, porcine and respiratory syndrome virus NA, porcine and respiratory syndrome virus EU, transmissible gastroenteritis coronavirus, foot and mouth disease virus, porcine epidemic diarrhea virus (S-INDEL and non-S-INDEL), swine influenza virus subtype H1N1, porcine circovirus type 2, seneca valley virus, porcine parvovirus, porcine deltacoronavirus and rotavirus...
June 21, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29920299/quantitative-multiplex-one-step-rt-pcr-assay-for-identification-and-quantitation-of-sabin-strains-of-poliovirus-in-clinical-and-environmental-specimens
#16
Hasmik Manukyan, Tatiana Zagorodnyaya, Ricardo Ruttimann, Yossi Manor, Ananda Bandyopadhyay, Lester Shulman, Konstantin Chumakov, Majid Laassri
An improved quantitative multiplex one-step RT-PCR (qmosRT-PCR) for simultaneous identification and quantitation of all three serotypes of poliovirus is described. It is based on using serotype-specific primers and fluorescent TaqMan oligonucleotide probes. The assay can be used for high-throughput screening of samples for the presence of poliovirus, poliovirus surveillance and for evaluation of virus shedding by vaccine recipients in clinical trials to assess mucosal immunity. It could replace conventional methods based on cell culture virus isolation followed by serotyping...
June 16, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29906491/recombinant-newcastle-disease-viruses-with-targets-for-pcr-diagnostics-for-rinderpest-and-peste-des-petits-ruminants
#17
P A van Rijn, J Boonstra, H G P van Gennip
Since February 1st 2011, rinderpest (RP) has been officially declared eradicated worldwide. National authorities have been requested to destroy all their RP related materials. Nonetheless, their national reference laboratories performing real time reverse transcription polymerase chain reaction assays (PCR diagnostics) need RP positive control samples, since some countries still prefer to maintain diagnostic capability for RP for several reasons. In the future, a similar situation will arise for peste des petits ruminants (PPR) as the ambition has been expressed to eradicate PPR...
June 12, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29902492/analytical-validation-of-viral-cns-flow-chip-kit-for-detection-of-acute-meningitis-and-encephalitis
#18
Mercedes Pérez-Ruiz, Irene Pedrosa-Corral, Sara Sanbonmatsu-Gámez, Cristina Gómez-Camarasa, José María Navarro-Marí
A new molecular assay (Viral CNS Flow Chip kit, Master Diagnóstica, Spain) has been developed for the detection of eight viruses causing acute meningitis and encephalitis, i.e. herpes simplex viruses 1-2, varicella zoster virus, human enterovirus, human parechovirus, Toscana virus, human cytomegalovirus and Epstein Barr virus. The new assay is a multiplex one-step RT-PCR followed by automatic flow-through hybridization, colorimetric detection and image analysis. The limit of detection was 50 copies/reaction, and 10 copies/reaction for human enterovirus and the other seven viruses, respectively...
June 12, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29902491/on-site-detection-of-bovine-leukemia-virus-by-a-field-deployable-automatic-nucleic-extraction-plus-insulated-isothermal-polymerase-chain-reaction-system
#19
Vickie J Ruggiero, Oscar J Benitez, Yun-Long Tsai, Pei-Yu Alison Lee, Chuan-Fu Tsai, Yu-Chun Lin, Hsiao-Fen Grace Chang, Hwa-Tang Thomas Wang, Paul Bartlett
Bovine leukemia virus (BLV) is a contagious, oncogenic deltaretrovirus of cattle with a worldwide distribution. In the US, over 40% of dairy cows are infected with the virus, and evidence of its economic impact is growing. This study evaluated the performance of a field-deployable automatic nucleic acid-extraction/insulated isothermal PCR (iiPCR) system for on-site BLV-proviral DNA detection in dairy cows compared with a conventional laboratory real-time PCR (rt-PCR). Assay performance was verified in parallel tests of 36 archived blood samples with 100% agreement (κ = 1...
June 11, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29894712/reverse-transcription-loop-mediated-isothermal-amplification-rt-lamp-designed-for-fast-and-sensitive-on-site-detection-of-pepper-chat-fruit-viroid-pcfvd
#20
Parichate Tangkanchanapas, Monica Höfte, Kris De Jonghe
Pepper chat fruit viroid (PCFVd) is one of the most important tomato and pepper diseases causing serious losses, affecting productivity, fruit quality and even international seed trade. Reverse transcription Loop-mediated isothermal amplification (RT-LAMP) is a fast and reliable RNA amplification assay, out competing conventional reverse transcription polymerase chain reaction (RT-PCR) in robustness, analytical sensitivity and specificity, and cost-effectiveness. In this work, a PCFVd specific RT-LAMP detection assay, based on a set of six primers was developed...
June 9, 2018: Journal of Virological Methods
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