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International Endodontic Journal

Shengzhe S, Yongna Z, Song L, Yan L, Jing Z
AIM: To investigate the role of cAMP response element-binding protein (CREB) in the regulation of odonto/osteogenic differentiation of stem cells from the apical papilla (SCAPs). METHODOLOGY: SCAPs were obtained from human impacted third molars (n=15). Isolated SCAPs were transfected with CREB overexpressing/silenced lentivirus. Transfected cells were stained with alizarin red to investigate mineralized nodule formation. The expression of the mineralization-related genes alkaline phosphatase (ALP), collagen type I (Col I), runt-related transcription factor 2 (RUNX2), osterix (OSX), and osteocalcin (OCN), was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR)...
October 7, 2016: International Endodontic Journal
I Kaşikçi Bilgi, I Köseler, P Güneri, M Hülsmann, M K Çalişkan
AIM: To compare the amount of apically extruded debris and of remaining filling material during the removal of root canal filling material using three rotary NiTi retreatment instruments or Hedström files. METHODOLOGY: Ninety-six severely curved human molars of both jaws were selected. The root canals were prepared to size X2 (tip size 25, .06 taper) using the ProTaper Next system (Dentsply Sirona, Ballaigues, Switzerland), filled with gutta-percha and AH Plus sealer (Dentsply De Trey, Konstanz, Germany) and were randomly divided into 4 experimental groups (n= 24 each) with two subgroups of maxillary and mandibular teeth each...
October 5, 2016: International Endodontic Journal
F Ferreira, I Barbosa, P Scelza, D Russano, J Neff, M Montagnana, M Zaccaro Scelza
AIM: To describe a new method for the assessment of nanoscale alterations in the surface topography of nickel-titanium endodontic instruments using a high-resolution optical method, and to verify the accuracy of the technique. METHODOLOGY: Noncontact three-dimensional optical profilometry was used to evaluate defects on a size 25, .08 taper reciprocating instrument (WaveOne(®) ), which was subjected to a cyclic fatigue test in a simulated root canal in a clear resin block...
October 1, 2016: International Endodontic Journal
G De-Deus, F G Belladonna, E J N L Silva, E M Souza, J C A Carvalhal, R Perez, R T Lopes, M A Versiani
AIM: To evaluate the frequency of dentinal micro-cracks after root canal filling procedures with GuttaCore (GC), cold lateral compaction (CLC) and warm vertical compaction (WVC) techniques in mandibular molars using micro-computed tomographic analysis. METHODOLOGY: Thirty mesial roots of mandibular molars, with a type II Vertucci's canal configuration, were prepared to working length with a Reciproc R40 instrument and randomly assigned to one of 3 experimental groups (n = 10), according to the technique used for root filling: GC, CLC or WVC...
September 30, 2016: International Endodontic Journal
P Linsuwanont, P Sinpitaksakul, T Lertsakchai
AIM: To investigate the response of immature permanent teeth with non-vital pulps after revitalization procedures using cone beam computed tomography imaging and conventional radiograph. METHODOLOGY: Clinical records of patients who had undergone revitalization between 1997 and 2014 were collected. Seventeen patients attended the follow-up examination. Clinical examination and radiographic examination by means of CBCT imaging and conventional radiographs were conducted to evaluate treatment outcomes, especially the dimensional changes of the root and the pattern of root maturation...
September 30, 2016: International Endodontic Journal
Y-L Tsai, W-C Liao, C-Y Wang, M-C Chang, S-H Chang, S-F Chang, C-W Chang, Y-D Huang, C-P Chan, J-H Jeng
AIM: To describe the clinical characteristics and radiographic findings of horizontal root fractures (HRF) in posterior teeth without a history of dental trauma. METHODOLOGY: A total 24 patients and 31 HRF cases in 28 posterior teeth were collected from 2006 to 2015. Clinical examinations and radiographic imaging were evaluated. Value of confidence intervals of the proportions were calculated for data presentation. RESULTS: The number of males (54%) was similar to females (46%)...
September 28, 2016: International Endodontic Journal
M Collado-González, D García-Bernal, R E Oñate-Sánchez, P S Ortolani-Seltenerich, A Lozano, L Forner, C Llena, F J Rodríguez-Lozano
AIM: To evaluate the biocompatibility of three calcium silicate-based endodontic sealers, Bioroot BC Sealer (Septodont, Saint-Maur-des-Fosses, France), Endoseal MTA (EndoSeal, Maruchi, Seoul, Korea) and Nano Ceramic Sealer (B&L Biotech, Fairfax, VA, USA) (NCS) on human periodontal ligament stem cells (hPDLSCs). METHODOLOGY: hPDLSCs cells were cultured in the presence of various endodontic sealer eluates for 24h. Cell viability was determined using the MTT assay...
September 26, 2016: International Endodontic Journal
P Saumya-Rajesh, J Krithikadatta, N Velmurugan, C Sooriaprakas
AIM: This randomized controlled trial compared the incidence of post-instrumentation pain associated with Mtwo rotary NiTi files and the self-adjusting file system following canal shaping and cleaning. METHODOLOGY: Following sample size estimation, a total of 130 patients were randomized into two groups based on selection criteria [group Mtwo and group SAF (self-adjusting file)]. Root canal treatment was carried out in two appointments. The teeth were endodontically treated with the appropriate allotted systems following the similar clinical parameters...
September 23, 2016: International Endodontic Journal
S Patel, A Aldowaisan, A Dawood
AIM: This case report describes a new approach to isolation and soft tissue retraction during endodontic surgery using cone-beam computed tomography (CBCT), computer-aided design (CAD) and three-dimensional (3D) printing. SUMMARY: A 53-year-old patient presented for endodontic treatment of her maxillary left central incisor. It was decided to treat this tooth with a microsurgical approach. The data from the diagnostic CBCT scan were also used to make a physical model of the operative site, and CAD software was used to design a soft tissue retractor to be used during the patient's surgery...
September 15, 2016: International Endodontic Journal
A M Elnaghy, S E Elsaka
AIM: To assess and compare the mechanical properties of TRUShape (TRS) with several nickel-titanium rotary instruments. METHODOLOGY: Cyclic fatigue, torsional resistance, flexibility and surface microhardness of TRS (size 25/.06v taper), ProTaper Next X2 (PTN X2; size 25/.06 taper), ProTaper Gold (PTG F2; size 25/.08 taper) and ProTaper Universal (PTU F2; size 25/.08 taper) instruments were evaluated. The topographic structures of the fracture surfaces of instruments were assessed using a scanning electron microscope...
September 12, 2016: International Endodontic Journal
L T A Cintra, L L Ferreira, F Benetti, A A Gastélum, J E Gomes-Filho, E Ervolino, A L F Briso
AIM: To evaluate pulpal tissue response after dental bleaching in normal and alloxan-induced diabetic rats. METHODOLOGY: Twenty-eight rats were divided into two groups of normoglycaemic and diabetic rats (n = 14). Diabetes mellitus (DM) was induced with alloxan. After DM confirmation, all rats were anaesthetized and dental bleaching was performed with 35% hydrogen peroxide (H2 O2 ) on the right maxillary molars for 30 min. Left molars were used as controls. Bleaching resulted in four hemimaxillae groups: normoglycaemic (N), N-bleached (NBle), diabetic (D) and D-bleached (DBle)...
September 10, 2016: International Endodontic Journal
C B Hoppe, J A P Oliveira, F S Grecca, A N Haas, M S Gomes
AIM: To evaluate the association between chronic oral inflammatory burden (OIB) - as the combination of periodontal and endodontic disease load - and physical fitness. METHODOLOGY: One hundred and twelve nonsmoker male police officers who performed a standardized physical fitness test (PFT) were analysed. Participants underwent oral clinical and periapical radiographic examinations. Periodontal disease was assessed by probing depth (PD) and clinical attachment loss (AL)...
August 31, 2016: International Endodontic Journal
H M A Ahmed, M A Versiani, G De-Deus, P M H Dummer
Knowledge of root and root canal morphology is a prerequisite for effective nonsurgical and surgical endodontic treatments. The external and internal morphological features of roots are variable and complex, and several classifications have been proposed to define the various types of canal configurations that occur commonly. More recently, improvements in nondestructive digital image systems, such as cone-beam and micro-computed tomography, as well as the use of magnification in clinical practice, have increased the number of reports on complex root canal anatomy...
August 31, 2016: International Endodontic Journal
L M S Castro-Raucci, L N Teixeira, I R Oliveira, W Raucci-Neto, M Jacobovitz, A L Rosa, P T de Oliveira
AIM: To evaluate the effect of a calcium aluminate-based cement (CAC+) on the development of the osteogenic phenotype in vitro. METHODOLOGY: Rat calvaria-derived cells were grown on Thermanox(®) coverslips for 24 h and then exposed either to samples (4-hour set) of CAC+ or mineral trioxide aggregate (MTA) placed on Transwell(®) inserts for periods of up to 14 days. Non-exposed cultures were used as the controls. The comparisons were carried out using the non-parametric Kruskal-Wallis test, followed by the Student-Newman-Keuls post-hoc test when appropriate...
August 13, 2016: International Endodontic Journal
M S Gomes, D E Böttcher, R K Scarparo, R D Morgental, S B G Waltrick, A C Ghisi, N M Rahde, M G Borba, L C Blomberg, J A P Figueiredo
AIM: To determine the prevalence of preoperative endodontic pain (PREP) and the incidence of postoperative endodontic pain (POEP), identifying the predictors of PREP and POEP in a southern Brazilian subpopulation, using clinical data from an electronic chart database (ECD). METHODOLOGY: This retrospective observational study included 563 consecutive individuals presenting for root canal treatment (RCT). Patients were treated by undergraduate and graduate students, following standard RCT protocols...
August 13, 2016: International Endodontic Journal
E M Rodrigues, A L G Cornélio, L B Mestieri, A S C Fuentes, L P Salles, C Rossa-Junior, G Faria, J M Guerreiro-Tanomaru, M Tanomaru-Filho
AIM: To investigate the cytotoxicity, osteogenic bioactivity and mRNA expression of osteogenic markers of bone morphogenetic protein 2 (BMP-2), osteocalcin (OC) and alkaline phosphatase (ALP) induced by the extracts of set MTA Plus (MTA P) (Avalon Biomed Inc. Bradenton, FL, USA) in comparison with MTA (Angelus, Londrina, PR, Brazil) on human dental pulp cells (hDPCs). METHODOLOGY: Cell viability was assessed by mitochondrial dehydrogenase enzymatic (MTT) assay, and the mechanism of cell death was evaluated by flow cytometry...
August 13, 2016: International Endodontic Journal
T Turk, M E Kaval, M Sarikanat, M Hülsmann
To evaluate the effect of chlorhexidine (CHX) on fracture resistance of roots treated with different concentrations of ethylenediaminetetraacetic acid (EDTA) METHODOLOGY: One hundred and twenty intact single-rooted premolar teeth were sectioned below the cementum-enamel junction to standardize the length of the teeth to 12 mm. The canals of one hundred specimens were instrumented with ProTaper Universal rotary instruments up to size F4 and were randomly divided into five groups (n=20) according to the final irrigating solutions: Group 1: distilled water (DW), Group 2: 5% EDTA and 2...
July 28, 2016: International Endodontic Journal
K M Galler
Revitalization or regenerative treatment approaches in teeth with incomplete root formation and pulp necrosis have become part of the therapeutic endodontic spectrum and should be considered as an alternative to conventional apexification. Ideally, regenerative endodontic procedures allow not only for a resolution of pain, inflammation and periapical lesions, but also for the formation of an immunocompetent tissue inside the root canal which can reconstitute the original biological structure and function of dental pulp and thus lead to an increase in root length, and thickness and strength of previously thin, fracture-prone dentine walls...
October 2016: International Endodontic Journal
J Xu, M Y Shao, H Y Pan, L Lei, T Liu, L Cheng, T Hu, P M H Dummer
AIM: To develop a suitable protocol for screening extracted teeth so as to create anatomically well-balanced experimental groups for endodontic studies. METHODOLOGY: Sixty pairs (120 teeth) of contralateral premolars (CLPs) were collected. The evaluation of samples for anatomical features was performed in the following ways. First, the 120 teeth were divided into right and left sides (N = 60, each side), and the teeth of each side were screened individually according to regular inclusion criteria (parameters included mature apical foramen, canal type, canal curvature and tooth length)...
October 2016: International Endodontic Journal
M M Koçak, E Çiçek, S Koçak, B C Sağlam, F Furuncuoğlu
AIM: To evaluate the amount of apically extruded debris after root canal instrumentation with ProTaper Next and HyFlex instruments in curved root canals. METHODOLOGY: Forty extracted mandibular first molar teeth with curved mesial roots and of similar lengths were instrumented using ProTaper Next or HyFlex instruments. The extruded debris was collected into pre-weighed Eppendorf tubes. The tubes were stored in an incubator at 68 °C for 5 days. The tubes were weighed to obtain the final dry weight of the extruded debris...
October 2016: International Endodontic Journal
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