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International Endodontic Journal

G Gambarini, M Seracchiani, L Piasecki, F O Valenti, M Galli, D Di Nardo, L Testarelli
AIM: To compare two different clinical techniques when using endodontic rotary instruments by monitoring the torque in vivo. METHODOLOGY: Ten single rooted maxillary anterior teeth were prepared by an endodontist using size 25, .06 taper TF rotary instruments (KerrEndo, Orange, CA, USA). All instruments were rotated at 500 rpm with maximum torque set at 2.5Ncm using an endodontic motor (Kavo, Biberach, Germany), which automatically recorded the torque values every 1/10 of a second...
November 16, 2018: International Endodontic Journal
A G Machado, B P S Guilherme, J C Provenzano, M F Marceliano-Alves, L S Gonçalves, J F Siqueira, M A S Neves
AIM: To compare the effects of three instrumentation systems, and a supplementary approach with a finishing instrument, on filling material removal during retreatment of mandibular molar canals. METHODS: Sixty mesial canals from mandibular molars (Vertucci's type IV anatomy) were instrumented, filled and subjected to retreatment. After initial removal of the root canal filling material using the D-RaCe system, the canals were randomly distributed into 3 groups (n=20) according to the instrument system used for preparation: the Self-Adjusting File (SAF), TRUShape or XP-endo Shaper...
November 12, 2018: International Endodontic Journal
E J Kramer, H Meyer-Lueckel, T G Wolf, F Schwendicke, M Naumann, R J Wierichs
AIM: This prospective, non-interventional, multi-center, practice-based study aimed to evaluate the longevity of post-restored endodontically treated teeth (ETT) and to analyze factors influencing the success and survival of endodontic posts. METHODOLOGY: Eight general dental practitioners each placed up to 27 endodontic posts without any restriction to size and material. Teeth were restricted to incisors, canines and premolars.. Multi-level Cox proportional hazards models were used to evaluate the association between clinical factors and time until failure...
November 12, 2018: International Endodontic Journal
J Buchgreitz, M Buchgreitz, L Bjørndal
AIM: To report the precision of guided access cavity preparations in relation to demographic and dental variables in fifty patients. METHODOLOGY: This observational study was carried out during the period 2014-2017. The patients were consecutive referrals to a private endodontic practice. The inclusion criteria were: 1) pulp space obliteration associated with signs of apical periodontitis (PAI score >3 or sensitive to percussion, 2) teeth with pulp space obliteration in need of a post, where the referring dentist had attempted or failed to negotiate the obliterated root canal (and it was not possible to negotiate using the operating microscope within a reasonable time frame), 3) a surgical intervention was not justified...
November 8, 2018: International Endodontic Journal
G L Souza, C M P Rosatto, M J B Silva, M V Silva, D B R Rodrigues, C C G Moura
AIM: To evaluate the in vitro cytotoxicity and cytokine release of three fresh root canal sealers and to determine the type of cell death they induce. METHODOLOGY: The sealers tested were Sealer 26 (S26), AH Plus (AHP), and Endosequence BC Sealer (END). Fresh sealers were cultivated in contact with monocytes and polymorphonuclears (PMNs) obtained from peripheral blood of humans. Cell viability, apoptosis and necrosis were analysed at 4 h (PMNs) or 24 h (monocytes) using AnnexinV and propidium iodide in a cytometer...
November 2, 2018: International Endodontic Journal
A Zoya, R K Tewari, S K Mishra, S M Faisal, S Ali, A Kumar, S Moin
AIM: To evaluate the extraradicular peroxide release from sodium percarbonate compared to sodium perborate as an intracoronal bleaching agent. METHODOLOGY: Sixty mandibular single-rooted premolars with intact CEJ were selected. After root filling, gutta-percha was removed 4 mm apical to CEJ and 2 mm of GIC was condensed over the root filling. Intracoronal bleaching agents were placed into six groups of teeth (n=10): sodium perborate with distilled water (SPW); sodium percarbonate with distilled water (SPCW); sodium perborate with 30% hydrogen peroxide (SPHP); sodium percarbonate with 30% hydrogen peroxide (SPCHP); 30% hydrogen peroxide as positive control (HP) and distilled water as negative control (CL)...
November 2, 2018: International Endodontic Journal
W L O da Rosa, V P Lima, R R Moraes, E Piva, A F da Silva
The aim of this systematic review and meta-analysis was to evaluate whether the use of calcium hydroxide (CH) liner improves the clinical success in the treatment of deep caries lesions of primary and permanent teeth. The review was reported in accordance with the PRISMA Statement. Only studies that evaluated deep carious lesions treated with and without a CH liner were included. The required outcomes had to be obtained by clinical, radiographic or laboratory evaluations. Statistical analyses were performed with the RevMan 5...
November 2, 2018: International Endodontic Journal
B Gu, T Kaneko, S Y M Zaw, P P Sone, H Murano, Y Sueyama, Z C T Zaw, T Okiji
AIM: To assess M1/M2 macrophage phenotypes in a coronal pulp regeneration model in rats, under the hypothesis that there are dynamic M1/M2 phenotype changes during the different stages of the pulp regeneration. METHODOLOGY: The maxillary first molars of Wistar rats were pulpotomized, and biodegradable hydrogel-made scaffolds carrying rat bone marrow mesenchymal stem cells were implanted in the pulp chamber. After 3, 7, and 14 days, samples were processed for (1) histological analysis and double immunoperoxidase staining for CD68 (a general macrophage marker) and one of either CCR7 (an M1 marker), CD163 (an M2 marker), or CD206 (an M2 marker); (2) real-time PCR for AIF1 (an M1 marker), CD163, CD206, IL-10 and TNF-α mRNA expression; and (3) western blotting for detection of CD68, CCR7 and CD206 proteins...
November 2, 2018: International Endodontic Journal
X Huang, F Liu, J Hou, K Chen
AIM: To profile miRNA expression between inflamed and healthy human dental pulp tissues and to investigate how the up-regulation of miR-223-3p in the inflamed pulp tissue regulates odontoblast differentiation and regeneration. METHODOLOGY: Microarray analysis was used to identify differences in miRNA expression patterns between healthy and inflamed pulp tissue. The results were validated using quantitative real-time PCR. To determine the effect of miR-223-3p on odontoblast differentiation, miR-223-3p was overexpressed in human dental pulp stem cells (DPSCs), which were cultured in mineralizing induction medium (to induce odontoblast differentiation)...
October 28, 2018: International Endodontic Journal
C Plengwitthaya, K Dhanuthai, S Chantarangsu, C Ratisoontorn
AIM: To determine the prevalence of cholesterol crystals in periapical biopsies and to evaluate the correlation between cholesterol crystals in periapical biopsies and age, gender, location of the periapical lesion, pathologic diagnosis and lesion size. METHODOLOGY: The biopsy report database (Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand) during 2005-2014 was reviewed. The prevalence of cholesterol crystals in the periapical lesions of root filled teeth was determined...
October 19, 2018: International Endodontic Journal
A Torres, E Shaheen, P Lambrechts, C Politis, R Jacobs
AIM: To describe a minimally invasive method to create a 3D printed guide to gain access to obliterated root canals on the basis of CBCT data. SUMMARY: A 85-year-old female ASA III was referred for root canal treatment of tooth 22. Clinically there were no complains, no percussion pain or sinus tract. Radiologically the tooth had an obliterated canal with an apical radiolucency. The tooth was diagnosed with asymptomatic apical periodontitis. Microguided Endodontic treatment was performed with the help of a 3D printed guide...
October 19, 2018: International Endodontic Journal
R Philpott, K Gulabivala, R Leeson, Y-L Ng
AIMS: To investigate the prevalence, pain catastrophizing and other predictive factors, and clinical course of persistent pain/discomfort associated with teeth displaying periapical healing following non-surgical root canal treatment (NSRCT). METHODOLOGY: One-hundred-ninety-eight patients (264 teeth) who had NSRCT were reviewed at 5-14 months, post-operatively. Teeth with persistent post-treatment pain or discomfort, plus evidence of periapical healing were further monitored 0...
October 17, 2018: International Endodontic Journal
P Varela, E Souza, G de Deus, F Duran-Sindreu, M Mercadé
AIM: To compare histologically the debridement efficacy of four irrigation techniques in root canals instrumented with a reciprocating single file. METHODOLOGY: From recently extracted premolars with vital pulp, 68 were selected and classified as having round canals (mesiodistal diameter similar to buccolingual) or oval-shaped canals (mesiodistal diameter 2.5 times larger than buccolingual) (n = 32, each) by means of bidirectional radiographs. Four additional uninstrumented samples served as histological controls...
October 14, 2018: International Endodontic Journal
F H Busanello, X Petridis, M V R So, R J B Dijkstra, P K Sharma, L W M van der Sluis
AIM: To investigate the influence of biofilm structure on the biofilm removal capacity of endodontic irrigants and to study changes in the architecture of the remaining biofilms. METHODOLOGY: Streptococcus oralis J22 and Actinomyces naeslundii T14V-J1 were cocultured under different growth conditions on saliva-coated hydroxyapatite discs. A constant depth film fermenter (CDFF) was used to grow steady-state 4-day biofilms. Biofilms were grown under static conditions for 4 and 10 days within a confined space...
October 10, 2018: International Endodontic Journal
F Shafiei, M Doozandeh, F Gharibpour, A Adl
AIM: To assess the effect of acid-etching duration time on the compressive strength, microstructure and shear bond strength of All-Bond Universal adhesive to MTA-Angelus (AMTA), and Biodentine (BD). METHODOLOGY: Forty-eight cylindrical specimens (4 mm in diameter, 6 mm in height) of AMTA in four groups and 48 specimens of BD in four groups (n = 12) were prepared and stored for 72 h. The control groups were unetched whilst the three experimental groups were acid-etched for 5, 10 and 15 s, respectively, followed by compressive strength testing...
October 9, 2018: International Endodontic Journal
R Ordinola-Zapata, J N R Martins, M A Versiani, C M Bramante
AIM: To evaluate dentine thickness from both distal ('danger zone') and mesial ('safety zone') aspects of the MB1 and MB2 canals of maxillary first molars at the furcation level using micro-CT imaging. METHODOLOGY: One hundred maxillary first molars with two canals in the MB root were selected from a Brazilian subpopulation and scanned at a resolution of 19.6 μm in a micro-CT device. From each specimen, two axial cross-sectioned images were selected at 2 and 3 mm apical to the furcation level of the MB root and evaluated for the smallest dentinal thickness...
October 8, 2018: International Endodontic Journal
R C D Swimberghe, A De Clercq, R J G De Moor, M A Meire
AIM: To evaluate the efficacy of sonically, ultrasonically and laser-activated irrigation (LAI) in removing a biofilm-mimicking hydrogel from the isthmus in a root canal model. METHODOLOGY: Transparent resin blocks containing two standardized root canals (apical diameter of 0.3 mm, 6% taper, 16 mm long, with a coronal reservoir) connected by an isthmus (0.15 mm wide, 2 mm high) were used as the test model. The isthmus was filled with a hydrogel-containing dentine debris...
October 8, 2018: International Endodontic Journal
P A Francisco, M G Delboni, A R Lima, Y Xiao, W L Siqueira, B P F A Gomes
AIM: To characterize the proteome of 20 root canals in teeth with post-treatment endodontic disease using mass spectrometry and to correlate the identified proteins with clinical features. METHODOLOGY: Twenty patients with radiographic evidence of apical periodontitis and need for root canal re-treatment were selected. Samples from the root canal contents were collected and processed using two-dimensional capillary nano-flow liquid chromatography and electrospray ionization tandem mass spectrometry...
October 4, 2018: International Endodontic Journal
F F N P Costa, J Pacheco-Yanes, J F Siqueira, A C S Oliveira, I Gazzaneo, C A Amorim, P H B Santos, F R F Alves
AIM: To evaluate the frequency of post-treatment apical periodontitis associated with root filled teeth with at least one untreated root canal. METHODOLOGY: Eight hundred and seven cone beam computed tomography images containing at least one root filled tooth were selected from a collection of 1543 images from Brazilian individuals. Scans were taken using ICAT Classic devices (Imaging Sciences, Hatfield, PA, USA) in a private oral radiology clinic from January to April 2015...
October 4, 2018: International Endodontic Journal
C Kruse, R Spin-Neto, D C Evar Kraft, M Vaeth, L-L Kirkevang
AIM: To assess the diagnostic accuracy of Cone Beam Computed Tomography (CBCT) to diagnose apical periodontitis (AP) using histopathology of ex vivo human jaws as the reference standard. METHODOLOGY: Based on periapical radiographs of jaw specimens from human bodies donated for science, a sample of 223 teeth with 340 roots including all tooth groups, and different disease and treatment statuses was selected. Cone Beam Computed Tomography was performed using Cranex® 3Dx (Soredex Oy, Tuusula, Finland), small field-of-view (5 × 5 cm), and isotropic resolution 0...
September 28, 2018: International Endodontic Journal
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