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International Endodontic Journal

Sofía Tejada, Pilar Baca, Carmen María Ferrer-Luque, Matilde Ruiz-Linares, Mariano José Valderrama, María Teresa Arias-Moliz
AIM: To determine the free available chlorine of 2.5% sodium hypochlorite (NaOCl) alone and combined with 9% etidronic acid (HEDP) in the presence of inhibitors, organic tissue and organic tissue plus dentine debris; to evaluate the influence of dentine debris on the tissue-dissolving capacity of both NaOCl solutions; and to determine the antimicrobial action of these solutions when in contact with organic tissue and organic tissue plus dentine debris. METHODOLOGY: The available chlorine of the solutions over time in the absence and presence of the inhibitors was measured using a titration method...
July 18, 2018: International Endodontic Journal
I Vidovic-Zdrilic, A Vijaykumar, M Mina
AIM: To examine the contribution of perivascular cells expressing αSMA to reactionary dentinogenesis. METHODOLOGY: An inducible, Cre-loxP in vivo fate-mapping approach was used to examine the contribution of the descendants of cells expressing the αSMA-CreERT2 transgene to reactionary dentinogenesis in mice molars. Reactionary dentinogenesis was induced by experimental mild injury to dentine without pulp exposure. Student t-test was used to determine statistical significance at *p ≤ 0...
July 9, 2018: International Endodontic Journal
B Üreyen Kaya, C E Erik, E Sesli Çetin, M Köle, M Maden
AIM: To evaluate the bacterial reduction achieved by the WaveOne Gold (Dentsply Sirona Endodontics, Ballaigues, Switzerland), Hyflex EDM (Coltene-Whaledent, Altstätten, Switzerland), and XP-endo Shaper (FKG Dentaire SA, La Chaux-de-Fonds, Switzerland) in canals infected with E. faecalis. The null hypothesis (H0 ) was that there would be no difference among the NiTi systems in reducing the root canal bacterial load. METHODOLOGY: Seventy four mandibular premolar teeth with straight and round root canals were selected...
July 9, 2018: International Endodontic Journal
H Caballero-Flores, C K Nabeshima, E Binotto, M E L Machado
AIM: To assess the fracture incidence of instruments from single-file reciprocating systems when used by students in an endodontic graduate programme. METHODOLOGY: Dental records and periapical radiographs of patients treated by students on an endodontic graduate programme using reciprocating systems were assessed. Information on tooth type, number of treated root canals, number of fractured instruments, fragment size, and angle, radius, arc length and position of root canal curvature where fractures occurred were tabulated for analysis...
July 9, 2018: International Endodontic Journal
I Bago, M Suk, M Katić, D Gabrić, I Anić
AIM: To compare the Reciproc Blue, Reciproc, and ProTaper Universal Retreatment systems with regard to the effective removal of epoxy resin-based sealer and gutta-percha during the retreatment of oval, straight root canals. METHODOLOGY: Forty-five extracted, human mandibular first premolars with single straight oval canals were selected on the basis of cone beam computed tomography evaluations. The root canals were instrumented with the ProTaper Next system up to the X2 file, and filled with gutta-percha and epoxy resin-based sealer using the cold lateral condensation technique...
July 9, 2018: International Endodontic Journal
T Silva da Fonseca, G F Silva, J M Guerreiro-Tanomaru, E Sasso-Cerri, M Tanomaru-Filho, P S Cerri
AIM: To compare the formation of fibrous capsules around Biodentine and MTA Angelus implants as well as the participation of fibroblast growth factor-1 (FGF-1) and mast cells in the tissue response to these endodontic materials. METHODOLOGY: Sixty polyethylene tubes filled with Biodentine or MTA, and empty tubes (control group) were implanted into the dorsal subcutaneous tissues of male rats. After 7, 15, 30 and 60 days, the specimens were embedded in paraffin and the number of fibroblasts and mast cells was quantified in the sections stained with Masson's trichrome or Alcian Blue, respectively...
July 5, 2018: International Endodontic Journal
S Bürklein, J P Stüber, E Schäfer
AIM: To assess the real-time dynamic torque values and axial forces during preparation of straight root canals using three different endodontic motors and hand preparation. METHODOLOGY: Sixty maxillary incisors were divided into 4 homogeneous groups (n=15). After glide path establishment (size 20) the root canals were prepared to an apical size 40 using the rotary NiTi system F6 SkyTaper. Motors (DentaPort ZX OTR, EndoPilot and VDW.silver) were set to "auto-torque-reverse" mode and DentaPort ZX was set to its specific OTR mode (optimum torque reverse)...
July 3, 2018: International Endodontic Journal
C Wichnieski, K Maheshwari, L C Souza, F Nieves, T Tartari, G P Garlet, E Carneiro, A Letra, R M Silva
AIM: To investigate the DNA methylation profiles of immune-response related genes in apical periodontitis (AP) lesions. METHODOLOGY: The methylation profiles on the cytosine-phosphate-guanine (CpG) regions of 22 gene promoters involved in inflammation and autoimmunity were assessed in 60 human AP lesions and 24 healthy periodontal ligaments (controls) using a pathway-specific real-time polymerase chain reaction array (EpiTect® Methyl Signature PCR Array Human Inflammatory Response)...
June 14, 2018: International Endodontic Journal
S Ran, M Chu, S Gu, J Wang, J Liang
AIM: To investigate the ability of E. faecalis to cause apoptosis and pyroptosis of human osteoblastic MG63 cells and whether the inflammasome is involved in this process. METHODOLOGY: MG63 cells were infected with E. faecalis at a multiplicity of infection (MOI) of 10, 100, 500, and 1000 for 6 and 12 h. The cell proliferation was determined with the Cell Counting Kit-8. Apoptosis and pyroptosis after E. faecalis infection was evaluated by flow cytometry and a lactate dehydrogenase (LDH) cytotoxicity assay kit...
June 14, 2018: International Endodontic Journal
M Reymus, C Fotiadou, A Kessler, K Heck, R Hickel, C Diegritz
AIM: To assess the feasibility of producing artificial teeth for endodontic training using 3D printing technology, to analyse the accuracy of the printing process, and to evaluate the teeth by students when used during training. METHODOLOGY: Sound extracted human teeth were selected, digitalized by cone beam computed tomography (CBCT) and appropriate software and finally reproduced by a stereolithographic printer. The printed teeth were scanned and compared with the original ones (trueness) and to one another (precision)...
June 14, 2018: International Endodontic Journal
P Neelakantan, D R Herrera, V G A Pecorari, B P F A Gomes
AIM: The aim of this systematic review was to answer the following question: in patients with primary endodontic infection, is there a statistically significant difference in the endotoxin levels after chemomechanical preparation with sodium hypochlorite (NaOCl) or chlorhexidine (CHX)? METHODOLOGY: A protocol was prepared and registered on PROSPERO (CRD42017069996). Four electronic databases (MEDLINE via PubMeb, Scopus, Web of Science and Cochrane Library) were searched from their start dates to 1 March 2017 using strict inclusion and exclusion criteria and reviewed following PRISMA (Preferred Reporting Items for Systematic reviews and Meta-Analyses) guidelines...
June 9, 2018: International Endodontic Journal
W-Z Lew, S-W Feng, C-T Lin, H-M Huang
AIM: To investigate whether static magnetic fields (SMFs) have a positive effect on the migration and dentinogenesis of dental pulp stem cells (DPSCs) to promote reparative dentine formation. METHODOLOGY: In vitro scratch assays and a traumatic pulp exposure model were performed to evaluate the effect of 0.4-Tesla (T) SMF on DPSC migration. The cytoskeletons of the DPSCs were identified by fluorescence immunostaining and compared with those of a sham-exposed group...
June 5, 2018: International Endodontic Journal
T Tartari, C Wichnieski, L Bachmann, M Jafelicci, R M Silva, A Letra, R van der Hoeven, M A H Duarte, C M Bramante
AIM: To investigate the effects of combinations of several irrigants on the roughness and wettability of dentine, adhesion of Enterococcus faecalis and Candida albicans and adsorption of chlorhexidine (CHX) to the dentine. METHODOLOGY: Bovine dentine samples were prepared and their surface roughness standardized. The samples were distributed in groups (n = 10) and subjected to one of the following irrigation protocols: G1 - saline solution; G2 - sodium hypochlorite (NaOCl); G3 - NaOCl + ethylenediaminetetraacetic acid (EDTA); G4 - NaOCl + peracetic acid (PAA); G5 - NaOCl + 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP); G6 - NaOCl + EDTA + CHX; G7 - NaOCl + PAA + CHX; G8 - NaOCl + HEDP + CHX; and G9 - mixture of NaOCl + HEDP...
June 4, 2018: International Endodontic Journal
F Shahsavari, M Abbasnejad, S Esmaeili-Mahani, M Raoof
AIM: To investigate the role of rostral ventromedial medulla orexin-1 receptors in the modulation of orofacial nociception as well as nociception-induced learning and memory impairment in adult male rats. METHODOLOGY: Pulpal nociception was induced by intradental application of capsaicin (100 μg) into the incisors of rats. Orexin-1 receptors agonist (orexin-A, 10, 25 and 50 pmol L-1  rat-1 ) and antagonist (SB-334867-A, 40 and 80 nmol L-1  rat-1 ) were microinjected into the rostral ventromedial medulla prior to capsaicin administration...
June 1, 2018: International Endodontic Journal
K Makino, O Takeichi, K Imai, H Inoue, K Hatori, K Himi, I Saito, K Ochiai, B Ogiso
AIM: To determine whether Porphyromonas endodontalis can reactivate latent Epstein-Barr virus (EBV). METHODOLOGY: The concentrations of short-chain fatty acids (SCFAs) in P. endodontalis culture supernatants were determined using high-performance liquid chromatography. A promoter region of BamHI fragment Z leftward open reading frame 1 (BZLF-1), which is a transcription factor that controls the EBV lytic cycle, was cloned into luciferase expression vectors. Then, the luciferase assay was performed using P...
June 1, 2018: International Endodontic Journal
S Drukteinis, V Peciuliene, P M H Dummer, J Hupp
AIM: To evaluate and compare the canal shaping ability of BioRace, ProTaper NEXT and Genius engine-driven nickel-titanium (NiTi) file systems in extracted mandibular first molars using micro-computed tomography (MCT). METHODOLOGY: Sixty mesial root canals of mandibular first molars were randomly divided into three equal groups, according to the instrument system used for root canal preparation (n = 20): BioRace (BR), ProTaper NEXT (PTN) or Genius (GN). Root canals were prepared to the full WL using a crown-down technique up to size 35, ...
June 1, 2018: International Endodontic Journal
E İriboz, B Arıcan Öztürk, M Kolukırık, I Karacan, H Sazak Öveçoğlu
AIM: To detect the unknown components of the oral microbiome and the effects of root canal treatment in a Turkish population and to evaluate the changes in microbial diversity in the root canals before and after treatment. METHODOLOGY: Single-rooted central and lateral maxillary incisors with one canal were chosen from 20 patients. Baseline samples of intact intracanal microbiota were collected from 20 root canals of permanent teeth with necrotic pulps using sterile paper points...
June 1, 2018: International Endodontic Journal
G Sánchez-Sanhueza, H Bello-Toledo, G González-Rocha, A T Gonçalves, V Valenzuela, C Gallardo-Escárate
AIM: To determine the bacterial microbiota in root canals associated with persistent apical periodontitis and their relationship with the clinical characteristics of patients using next-generation sequencing (NGS). METHODOLOGY: Bacterial samples from root canals associated with teeth having persistent apical periodontitis were taken from 24 patients undergoing root canal retreatment. Bacterial DNA was extracted, and V3-V4 variable regions of the 16S rRNA gene were amplified...
May 22, 2018: International Endodontic Journal
J B Yong, S Sivarajan, P V Abbott
AIM: To assess whether the timing of pulp disease after tooth restoration was associated with type of restorative dental material used, extent of the restoration or tooth type. METHODOLOGY: A comprehensive search and analysis of data using the Titanium Oral Health Management software program at The Oral Health Centre of Western Australia were performed to correlate procedural codes for teeth that had restorations placed and subsequently developed pulp disease requiring endodontic treatment or extraction from 1st January 2009 to 31st December 2013...
May 19, 2018: International Endodontic Journal
S G Kim, M Malek, A Sigurdsson, L M Lin, B Kahler
The European Society of Endodontology and the American Association for Endodontists have released position statements and clinical considerations for regenerative endodontics. There is increasing literature on this field since the initial reports of Iwaya et al. (Dental Traumatology, 17, 2001, 185) and Banchs & Trope (Journal of Endodontics, 30, 2004, 196). Endogenous stem cells from an induced periapical bleeding and scaffolds using blood clot, platelet rich plasma or platelet-rich fibrin have been utilized in regenerative endodontics...
May 19, 2018: International Endodontic Journal
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