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International Endodontic Journal

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https://www.readbyqxmd.com/read/29786880/metagenomic-study-of-bacterial-microbiota-in-persistent-endodontic-infections-using-next-generation-sequencing
#1
G Sánchez-Sanhueza, H Bello-Toledo, G González-Rocha, A T Gonçalves, V Valenzuela, C Gallardo-Escárate
AIM: To determine the bacterial microbiota in root canals associated with persistent apical periodontitis and their relationship with the clinical characteristics of patients using next-generation sequencing (NGS). METHODOLOGY: Bacterial samples from root canals associated with teeth having persistent apical periodontitis were taken from 24 patients undergoing root canal retreatment. Bacterial DNA was extracted, and V3-V4 variable regions of the 16S rRNA gene were amplified...
May 22, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29779218/an-analysis-of-the-timing-and-materials-associated-with-pulp-disease-following-restorative-dental-treatment
#2
J B Yong, S Sivarajan, P V Abbott
AIM: To assess whether the timing of pulp disease after tooth restoration was associated with type of restorative dental material used, extent of the restoration, or tooth type. METHODOLOGY: A comprehensive search and analysis of data using the Titanium Oral Health Management software program at The Oral Health Centre of Western Australia was performed to correlate procedural codes for teeth that had restorations placed and subsequently developed pulp disease requiring endodontic treatment or extraction from 1st January 2009 to 31st December 2013...
May 19, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29777616/regenerative-endodontics-a-review
#3
REVIEW
S G Kim, M Malek, A Sigurdsson, L M Lin, B Kahler
The European Society of Endodontology and the American Association for Endodontists have released position statements and clinical considerations for regenerative endodontics. There is increasing literature on this field since the initial reports of Iwaya et al. (2001) and Banchs & Trope (2004). Endogenous stem cells from an induced periapical bleeding and scaffolds using blood clot, platelet rich plasma or platelet rich fibrin have been utilized in regenerative endodontics. This approach has been described as a 'paradigm shift" and considered the first treatment option for immature teeth with pulp necrosis...
May 19, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29774548/release-of-tgf-%C3%AE-1-into-root-canals-with-various-final-irrigants-in-regenerative-endodontics-an-in-vitro-analysis
#4
Y Chae, M Yang, J R Kim
AIM: To investigate the release of growth factors into the root canal space after various final irrigants during regenerative endodontic procedures. The residual cytotoxic effect of final irrigants on stem cells from the apical papilla (SCAP) was also examined. METHODOLOGY: To measure the release of TGF-β1, root segments (8mm long) were irrigated with 1.5% NaOCl followed by 20 mL of final irrigants; Saline, 17% EDTA, 10% citric acid, 10% or 37% phosphoric acid...
May 17, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29772080/substance-p-and-calcitonin-gene-related-peptide-expression-in-human-periodontal-ligament-after-root-canal-preparation-with-reciproc-blue-waveone-gold-xp-endoshaper-and-hand-files
#5
J Caviedes-Bucheli, N Rios-Osorio, M Rey-Rojas, F Laguna-Rivero, M M Azuero-Holguin, L E Diaz, H Curtidor, J J Castaneda-Ramirez, H R Munoz
AIM: To quantify the Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. METHODOLOGY: Fifty human periodontal ligament samples were obtained from healthy mandibular premolars where extraction was indicated for orthodontic reasons. Prior to extraction, 40 of these premolars were equally divided into four groups, and root canals were prepared using four different systems: Reciproc Blue, WaveOne Gold, XP EndoShaper, and a hand instrumentation technique...
May 17, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29763971/evaluation-of-the-effects-of-the-solution-used-for-electrochemical-dissolution-of-nickel-titanium-endodontic-files-on-dentine-structure-microhardness-and-cell-viability
#6
C C F Amaral, F Ormiga, L C Boldrini, P G Miranda, T A Mendonça, J M Granjeiro, J A C P Gomes
AIM: To evaluate the effects of the [NaF 12 g/L + NaCl 1 g/L] solution used in the electrochemical dissolution process of fractured endodontic files, as well as its NiTi-containing product, on dentine hardness, topography and human fibroblast viability. METHODOLOGY: Sixty single-rooted human teeth were evaluated for dentine microhardness using the Vickers hardness test and the area and number of dentinal tubules by scanning electron microscopy. The samples were divided according to the dentine surface treatment: distilled water; 17% EDTA; [NaF 12 g/L + NaCl 1 g/L]; and 17% EDTA + [NaF 12 g/L + NaCl 1 g/L]...
May 15, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29737548/prevotella-strains-and-lactamic-resistance-gene-distribution-in-different-oral-environments-of-children-with-pulp-necrosis
#7
B Roese de Lima, G F Nicoloso, C C Fatturi-Parolo, M B C Ferreira, F Montagner, L Casagrande
AIM: To determine the presence of Prevotella species, the cfxA/cfxA2, blaZ and blaTEM genes associated with resistance to lactamic agents in different oral niches of children with pulp necrosis. METHODOLOGY: Children with pulp necrosis in primary teeth had samples of saliva, supragingival, pulp chamber and root canal biofilms collected and tested for Prevotella species (P. intermedia, P nigrescens, P. tannerae) and for beta-lactam resistance genes (cfxA / cfxA2, blaZ and blaTEM)...
May 8, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29737545/influence-of-apical-enlargement-on-the-repair-of-apical-periodontitis
#8
C M Jara, R C Hartmann, D E Böttcher, T S Souza, M S Gomes, J A P Figueiredo
AIM: To evaluate the influence of several apical enlargement protocols on the radiographic and histological healing of apical periodontitis in rats. METHODOLOGY: Apical periodontitis was induced bilaterally in the mandibular right and left first molars of 24 Wistar rats by pulp exposure to the oral cavity for three weeks. A standard serial root canal preparation technique was performed in the molar of one side, while the opposite side was the control group. Rats were randomly divided into three experimental groups (n=8), according to the diameter of apical enlargement during root canal preparation: K-files size 20 (EG1), size 25 (EG2), and size 30 (EG3)...
May 8, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29737544/external-cervical-resorption-part-2-management
#9
REVIEW
S Patel, F Foschi, R Condon, T Pimentel, B Bhuva
Effective management of external cervical resorption (ECR) depends on accurate assessment of the true nature and accessibility of ECR; this has been discussed in part 1 of this 2 part paper. This aim of this paper is firstly, to review the literature in relation to the management of ECR, and secondly, based on the available evidence describe different strategies for the management of ECR. In cases where ECR is supracrestal, superficial and with limited circumferential spread a surgical repair without root canal treatment is the preferred approach...
May 8, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29730894/cytokine-expression-in-response-to-root-repair-agents
#10
R R Oliveira, W L F Tavares, A L Reis, V A Silva, L Q Vieira, A P Ribeiro Sobrinho
AIM: To evaluate the expression of TNF-α, IL-6, IFN-γ, TGF-β, IL-4, IL-10, RANKL, RANK and OPG on mouse calvarial bone treated with MTA, Geristore® and Emdogain® . METHODOLOGY: Bone wounds were made on the heads of C57BL/6 mice, breaking the periosteum and the cortical surface of the calvaria. Each repair agent was inserted into sectioned Eppendorf microtubes and placed on the bone wound, and soft tissues were sutured. At 14 and 21 days, animals were sacrificed and the treated region was dissected...
May 6, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29729097/3d-printed-model-for-hands-on-training-in-dental-traumatology
#11
M Reymus, C Fotiadou, R Hickel, C Diegritz
AIM: To assess the feasibility of creating a realistic model for hands-on training in dental traumatology using 3D printing technology, and then to investigate the added value of working with the website dentaltraumaguide. org. METHODOLOGY: With the use of special software applications, a model was designed based on the CBCT of the maxilla of a real patient that imitated several traumatic dental injuries. The model was reproduced using a stereolithographic printer in order to use the specimens in a hands-on training course on dental traumatology for undergraduate students in their final year in the Department of Conservative Dentistry and Periodontology in Munich, Germany...
May 5, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29729026/longevity-of-bond-strength-of-resin-cements-to-root-dentine-after-radiation-therapy
#12
P A Yamin, R D Pereira, F C Lopes, A M Queiroz, H F Oliveira, P C Saquy, M D Sousa-Neto
AIM: To evaluate the bond strength and adhesive interface between several resin cements and root dentine immediately and after 6 months of radiotherapy protocol. METHODOLOGY: Sixty maxillary canines were selected and randomly assigned to two groups (n=30): one group was not irradiated, and the other one was subjected to a cumulative radiation dose of 60 Gy. The teeth were sectioned to obtain roots with 16 mm length, and the canals were prepared with Reciproc system (R50) and filled using a lateral condensation technique with epoxy resin sealer...
May 4, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29704466/external-cervical-resorption-part-1-histopathology-distribution-and-presentation
#13
REVIEW
S Patel, A Mavridou, P Lambrechts, N Saberi
External Cervical Resorption (ECR) is the loss of dental hard tissue as a result of odontoclastic action. It is a dynamic process that involves periodontal, dental and in later stages pulpal tissues. Over the last two decades ECR has attracted increased interest, this is in part due to novel micro-CT and histopathological techniques for its assessment, and also improved radiographic detection using CBCT. This literature review will cover the aetiology, potential predisposing factors, histopathology and diagnosis of ECR...
April 28, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29704465/association-of-manual-or-engine-driven-glide-path-preparation-with-canal-centring-and-apical-transportation-a-systematic-review
#14
REVIEW
R C Hartmann, O A Peters, Jap de Figueiredo, G Rossi-Fedele
The role and effect of glide path preparation in root canal treatment remains controversial. This systematic review aims to compare apical transportation and canal centring of different glide path preparation techniques, with or without subsequent engine-driven root canal preparation. A database search in PubMed, PubMed Central, Embase, Scopus, EBSCO Dentistry & Oral Sciences Source and Virtual Health Library was conducted, using appropriate key words to identify the effect of glide path preparation (or its absence) on apical transportation and canal centring...
April 28, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29682752/mineral-density-in-carious-dentine-after-treatment-with-calcium-silicates-and-polyacrylic-acid-based-cements
#15
P M Pires, T P Santos, A Fonseca-Gonçalves, M M Pithon, R T Lopes, A A Neves
AIM: To test the hypothesis that polyacrylic acid-based and calcium silicate materials are able to increase mineral density of artificially demineralized dentine produced by a microcosm model for caries formation. METHODOLOGY: Forty-five dentine cavities were prepared in 9 sound human third molars (5 in each tooth). Specimens received a bacterial inoculum and were incubated in BHI plus 5% of sucrose for 96h to allow biofilm formation. After that, the specimens were scanned in a high energy micro-CT (baseline stack) and then, each cavity was filled with different cements: zinc polycarboxylate (Poly Zinc™ , Prevest DenPro, Jammu, India), conventional glass ionomer (Ketac Molar, 3M ESPE, Seefeld, Germany) and two calcium silicate based materials (MTA, Angelus, Londrina, Brazil and Biodentine™ , Septodont, Saint Maur des Fosses, France) while the central cavity was filled with utility wax (negative control)...
April 22, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29679496/detection-of-bone-marrow-derived-fibrocytes-in-human-dental-pulp-repair
#16
N Yoshiba, N Edanami, A Tohma, R Takeuchi, N Ohkura, A Hosoya, Y Noiri, H Nakamura, K Yoshiba
AIM: To explore the expression profile of CD45+/pro-collagen I+ fibrocytes in intact dental pulps as well as during wound healing in adult dental pulp tissue. METHODOLOGY: A total of 16 healthy permanent teeth were obtained from young patients (18 to 25 years) undergoing orthodontic treatment. Routine pulp capping with mineral trioxide aggregate (MTA) was performed under local anaesthesia to induce a mineralized barrier at the exposed surface. Teeth were extracted from patients after 7, 14, and 35 days...
April 21, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29645286/effect-of-curing-conditions-on-physical-and-chemical-properties-of-mta
#17
T Zarra, T Lambrianidis, L Vasiliadis, C Gogos
AIM: To evaluate setting time, pH, solubility and surface roughness of MTA+ and ProRoot MTA and characterize their hydration under several curing conditions. METHODOLOGY: Specimens were prepared to evaluate setting time (n=6 for each group, specimen dimensions 10x8x5mm), solubility (n=6 for each group, specimen dimension 20 mm in diameter and 1.5 mm thick) after 1 and 28 days, pH (n=10 for each group, specimen dimensions 10 mm in diameter and 1 mm thick) after 1, 7, 14, 21 and 28 days and surface roughness (n=10 for each group, specimen dimensions 4 mm in diameter and 3 mm high) after 28 days when cements were cured at 95% humidity or immersed in saline or HBSS at 37°C...
April 12, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29637579/defect-propagation-in-niti-rotary-instruments-a-noncontact-optical-profilometry-analysis
#18
I Barbosa, F Ferreira, P Scelza, J Neff, D Russano, M Montagnana, M Zaccaro Scelza
AIM: To evaluate the presence and propagation of defects and their effects on surfaces of nickel-titanium (NiTi) instruments using noncontact, three-dimensional optical profilometry, and to assess the accuracy of this method of investigation. METHODOLOGY: The flute surface areas of instruments from two commercial instrumentation systems, namely Reciproc R25 (n = 5) and WaveOne Primary (n = 5), were assessed and compared before and after performing two instrumentation cycles in simulated root canals in clear resin blocks...
April 10, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29635697/2-3-5-4-tetrahydroxystilbene-2-o-%C3%AE-glucoside-thsg-potentiates-self-renewal-of-human-dental-pulp-stem-cells-via-the-ampk-erk-sirt1-axis
#19
C-Y Lin, Y-T Chin, P-J Kuo, H-W Lee, H-M Huang, H-Y Lin, I-T Weng, C-N Hsiung, Y-H Chan, S-Y Lee
AIM: To evaluate the effect of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) on cell proliferation and examine the mechanisms of THSG-enhanced proliferative potential in human dental pulp stem cells (hDPSC). METHODS: After treatment with THSG, hDPSC were collected. Cell viability was determined by MTS assay, while messenger RNA (mRNA) expressions of proliferation and stem cell markers were analyzed using real-time PCR. Flow cytometry was also conducted to analysis protein expression of stem cell markers...
April 10, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29618163/differential-diagnosis-between-a-granuloma-and-radicular-cyst-effectiveness-of-magnetic-resonance-imaging
#20
G Lizio, E Salizzoni, M Coe, M R Gatto, S Asioli, T Balbi, G A Pelliccioni
AIM: To investigate the diagnostic reliability and accuracy of magnetic resonance imaging (MRI) to differentiate periapical lesions of endodontic origin and to compare the results with histopathological information. METHODOLOGY: The radiolucent periapical jaw lesions of 34 patients, which were surgically enucleated, were investigated by two radiologists using MRI, based on the same six criteria, to categorize the lesions as granulomas, radicular cysts or others...
April 4, 2018: International Endodontic Journal
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