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Experimental Lung Research

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https://www.readbyqxmd.com/read/29847180/the-effect-of-captopril-on-lipopolysaccharide-induced-lung-inflammation
#1
Javad Boskabadi, Amin Mokhtari-Zaer, Azam Abareshi, Mohammad Reza Khazdair, Bahman Emami, Nama Mohammadian Roshan, Mahmoud Hosseini, Mohammad Hossein Boskabady
PURPOSE: As an angiotensin converting enzyme (ACE) inhibitor, the effects of captopril on inflammation has been previously examined. Captopril has been shown to have anti-inflammatory and antioxidant effects. Imbalance in the oxidant/antioxidant system is one of the major causes of inflammation. In the present study, the effects of captopril on total and differential white blood cells (WBC), oxidative stress andlung histopathological changes produced by lipopolysaccharide (LPS) were investigated in rat...
May 30, 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29847197/changes-in-mucin-1-expression-in-a-rat-model-of-allergic-airway-inflammation
#2
Bilal Hasan, Haimiti Abudulimu, Li Tian, Abduxukur Ablimit
PURPOSE: To explore the distribution and expressional changes of mucin 1 (Muc1) in airway of rats with allergic airway inflammation. MATERIALS AND METHODS: Ovalbumin (OVA) was used to induce acute allergic inflammation in male Wistar rats. The distributions and expressions of Muc1 in lungs of normal and model rats were assessed by immunohistochemical staining and western blotting, respectively. RESULTS: Immunohistochemical staining showed that Muc1 distributed in airway epithelial cells with ciliates, but not those nonciliated cells...
April 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29781747/sialylation-pattern-in-lung-epithelial-cell-line-and-siglecs-expression-in-monocytic-thp-1-cells-as-cellular-indicators-of-cigarette-smoke-induced-pathology-in-vitro
#3
Przemyslaw Wielgat, Emil Trofimiuk, Robert Czarnomysy, Adam Holownia, Jan J Braszko
PURPOSE: Cellular response to cigarette smoke (CS) involves activation of recognition receptors resulting in changes in immune status, oxidative stress and cell turnover. We investigated the effects of CS on sialic acid-binding immunoglobulin type lectins (Siglecs) expression and their sialylated ligands in human immune and non-immune cells. METHODS: Human monocytes (THP-1) and epithelial cells (A549) were cultured in CS-conditioned medium (CSM). Expression of Siglec-8 and Siglec-5/Siglec-14 was analysed in THP-1 cells using flow cytometry...
April 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29737931/rux-largely-restores-lungs-in-iraq-pm-exposed-mice-up-regulating-regulatory-t-cells-tregs
#4
David Lin, Jonathan Li, Rabail Razi, Niha Qamar, Laurie Levine, Thomas Zimmerman, Sayyed A Hamidi, Millicent Schmidt, Marc G Golightly, Todd Rueb, Andrea Harrington, Merrill Garnett, Frank Antonawich, Steven McClain, Edmund Miller, Courtney Cox, Po Hsuan Huang, Anthony M Szema
Background Military personnel post-deployment to Iraq and Afghanistan have noted new-onset respiratory illness. This study's primary objective was to further develop an animal model of Iraq Afghanistan War Lung Injury (IAW-LI) and to test a novel class of anti-injury drug called RuX. Methods Particulate Matter (PM) samples were obtained in Iraq then characterized by spectromicroscopy. C57BL/6 mice underwent orotracheal instillation with PM, followed by drinkable treatment with RuX. Lung histology, inspiratory capacity (FlexiVent), thymic/splenic regulatory T cell (Treg) number, and whole-lung genomics were analyzed...
April 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29683754/micrornas-in-idiopathic-pulmonary-fibrosis-new-research-progress-and-their-pathophysiological-implication
#5
Chenggui Miao, Youyi Xiong, Guoxue Zhang, Jun Chang
Many studies have shown that microRNAs (miRNAs) play important roles in the development of idiopathic pulmonary fibrosis (IPF). The purpose of this review is to systematically summarize the recent advance of miRNAs in the pathology of IPF, highlighting the new research progress and their pathophysiological implication. Recent studies have shown that miRNAs differentially expressed in blood and lung tissue from IPF patients are closely related to the occurrence of IPF disease, which may be IPF diagnostic markers and prognostic indicators...
April 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29683731/bronchiolitis-obliterans-murine-model-induced-by-nitric-acid-aerosol-inhalation-an-economical-and-reproducible-model
#6
Jianing Yin, Xiaobo Ma, Fei Huang, Yucong Ma, Yanan Li
PURPOSE: Bronchiolitis obliterans (BO) is a highly debilitative and fatal syndrome associated with a series of severe lower airway disorders. The pathogenesis of BO is complicated and not entirely understood. An appropriate animal model of BO may aid research into its pathogenesis. Here, we establish a mouse model of BO to provide insight into this disease. MATERIALS AND METHODS: 6-8 week old BABL/c mice were exposed to 5% nitric acid (NA) aerosol through a nebulizer for 3 hours, and controls were exposed to distilled water instead...
April 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29677457/improved-protocol-for-simultaneous-analysis-of-leukocyte-subsets-and-epithelial-cells-from-murine-and-human-lung
#7
Christine Happle, Lena Meyer-Decking, Anika Dreier, Martin Wetzke, Stephanie Gläsener, Ruth Grychtol, Peter Braubach, Alexandra Jablonka, Sibylle Haid, Adan Chari Jirmo, Anika Habener, Jelena Skuljec, Gesine Hansen
PURPOSE: To study and isolate lung cells by flow cytometry, enzymatic digestion and generation of single cell suspensions is required. This significantly influences expression of cellular epitopes and protocols need to be adapted for the best isolation and subsequent analysis of specific cellular subsets. MATERIALS AND METHODS: We optimized protocols for the simultaneous isolation and characterization of specific human and murine lung cell types. For alveolar epithelial cells (AEC), a primarily dispase based digestion method and for leukocytes, a primarily collagenase based technique was adapted...
April 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29621420/regulation-of-eosinophil-recruitment-and-allergic-airway-inflammation-by-heparan-sulfate-proteoglycan-hspg-modifying-enzymes
#8
Xiao Na Ge, Idil Bastan, Sung Gil Ha, Yana G Greenberg, Jeffrey D Esko, Savita P Rao, P Sriramarao
BACKGROUND: HSPGs are glycoproteins containing covalently attached heparan sulfate (HS) chains which bind to growth factors, chemokines, etc., and regulate various aspects of inflammation including cell recruitment. We previously showed that deletion of endothelial N-acetylglucosamine N-deacetylase-N-sulfotransferase-1 (Ndst1), an enzyme responsible for N-sulfation during HS biosynthesis, reduces allergic airway inflammation (AAI). Here, we investigated the importance of O-sulfation mediated by uronyl 2-O-sulfotransferase (Hs2st) in development of AAI relative to N-sulfation...
March 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29565741/tuberculosis-and-cigarette-smoke-exposure-an-update-of-in-vitro-and-in-vivo-studies
#9
Y López-Hernández, C E Rivas-Santiago, J A López, G Mendoza-Almanza, R Hernandez-Pando
Tuberculosis (TB) has been declared the first cause of death by an infectious agent. Annually, 10.4 million people suffer active TB. Most infected individuals live in low-income countries, where social and economic conditions enhance the dissemination and progression of the disease. These countries have a high percentage of smokers. Thousands of studies have linked cigarette smoke (CS) with increased risk of many diseases, such as cancer and lung diseases. Numerous in vitro studies have been conducted to evaluate the general and specific toxic effects of CS in lung immune function...
March 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29543496/microrna-21-aggravates-chronic-obstructive-pulmonary-disease-by-promoting-autophagy
#10
Zhengpeng Zeng, ShengYang He, JunJuan Lu, Chun Liu, Hua Lin, ChaoQun Xu, LiHua Xie, ShengHua Sun
MicroRNAs and autophagy play important roles in chronic obstructive pulmonary disease (COPD). This study was designed to explore the role of microRNA-21 (miR-21) induced autophagy in COPD. Using the C57BL/6, miR-21-/- mice and human bronchial epithelial (16HBE) cell line, we found that in the lung tissues of mice, the level of autophagy in the COPD model group was significantly higher than that in the control group. However, compared to the COPD model, the level of autophagy was significantly lower in the miR-21-/- CSE+CS group...
March 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29419339/emphysema-induced-by-elastase-alters-the-mrna-relative-levels-from-dna-repair-genes-in-acute-lung-injury-in-response-to-sepsis-induced-by-lipopolysaccharide-administration-in-wistar-rats
#11
Luiz Philippe S Sergio, Leda M F Lucinda, Maycon M Reboredo, Flavia de Paoli, Lídia M C Fonseca, Bruno V Pinheiro, Andre L Mencalha, Adenilson S Fonseca
Purpose/Aim of the study: Patients suffering from chronic obstructive pulmonary disease (COPD) in association with acute respiratory distress syndrome (ARDS) present oxidative stress in lung cells, with production of free radicals and DNA lesions in pulmonary and adjacent cells. Once the DNA molecule is damaged, a set of enzymatic mechanisms are trigged to preserve genetic code integrity and cellular homeostasis. These enzymatic mechanisms include the base and the nucleotide excision repair pathways, as well as telomere regulation...
March 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29393707/kaempferol-attenuates-acute-lung-injury-in-caecal-ligation-and-puncture-model-of-sepsis-in-mice
#12
Dipankar Jyoti Rabha, Thakur Uttam Singh, Soya Rungsung, Tarun Kumar, Subhashree Parida, Madhu Cholenahalli Lingaraju, Avishek Paul, Monalisa Sahoo, Dinesh Kumar
AIM OF THE STUDY: Kaempferol is a flavonoid and important part of the diet. Kaempferol has shown antioxidant, antiinflammatory and antidiabetic activities in various studies. However, protective potential of kaempferol in acute lung injury induced by sepsis and its mechanism remains unclear. The present study was undertaken to evaluate the effect of kaempferol in sepsis-induced acute lung injury in mice and its possible mechanism of action. MATERIALS AND METHODS: Acute lung injury was induced by CLP surgery in mice...
March 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29381088/low-dose-monocrotaline-causes-a-selective-pulmonary-vascular-lesion-in-male-and-female-pneumonectomized-rats
#13
Daniel J Lachant, David F Meoli, Deborah Haight, Jason A Lyons, Robert F Swarthout, R James White
Purpose/Aim: Low doses (30-80 mg/kg) of monocrotaline are commonly used to create experimental models of pulmonary hypertension in rats. At these doses, monocrotaline causes pulmonary endothelial apoptosis and acute lung injury which ultimately results in pulmonary vascular disease. Higher doses of monocrotaline (300 mg/kg) are known to create severe liver injury, but previous investigations with lower doses have not reported histology in other organs to determine whether the vascular injury with monocrotaline is pulmonary-selective or generalized...
February 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29373054/erratum
#14
Yun Zeng
No abstract text is available yet for this article.
February 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29324063/granulomatous-lung-inflammation-is-nanoparticle-type-dependent
#15
Yves Pacheco, Marine Ponchon, Serge Lebecque, Alain Calender, Jean François Bernaudin, Dominique Valeyre, Marc Iglarz, Daniel S Strasser, Rolf Studer, Diego Freti, Toufiq Renno, Abederrazzaq Bentaher
BACKGROUND: Nanoparticles are increasingly suspected as a strong etiologic factor of granuloma formation. AIM OF THE STUDY: The aim of our study was to compare lung inflammatory response and histology changes following exposure of mice to two widely used nanoparticles: carbon nanotubes (MWCNT) and cadmium-based nanoparticles (QDOT705) in an attempt to better our understanding of granulomatous inflammation. MATERIALS AND METHODS: Various groups of mice were included: control mice and mice that were intranasally instilled with QDOT or MWCNT...
February 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29324062/four-day-pulse-of-sodium-cromoglycate-modulates-pulmonary-vessel-wall-remodeling-during-21-day-hypoxia-in-rats
#16
Tomáš Novotný, Jiří Uhlík, Luděk Vajner
AIM OF THE STUDY: Remodeling of pulmonary resistance arteries in rats due to 4-day hypoxia could be successfully suppressed by sodium cromoglycate. In this study, we tested the difference in the suppression between two distinct time patterns of cromoglycate administration during 21-day hypoxia. In the experiment, we focused on some details in both smooth muscle cells and extracellular matrix of pulmonary arterial walls. METHODS: During 21-day hypoxia, rats were treated with sodium cromoglycate either in the first four days or in the last four days...
February 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29324052/the-expression-profile-of-claudin-family-members-in-the-developing-mouse-lung-and-expression-alterations-resulting-from-exposure-to-secondhand-smoke-shs
#17
Joshua B Lewis, Felix R Jimenez, Brigham J Merrell, Brent Kimbler, Juan A Arroyo, Paul R Reynolds
Claudins are tight junctional proteins implicated in cell polarity and epithelial barrier maintenance. Claudin misregulation adversely impacts developmental aspects of cell differentiation and proliferation. The current research evaluated transcriptional expression for Claudins 1-11 and 18 in the developing murine lung at embryonic days (E) 14.5, 16.5, and 18.5 and at post-natal day (PN) 3 and PN15. Mouse lungs were also assessed by immunohistochemical analysis to qualitatively evaluate Claudin protein expression...
February 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29324051/anti-il-8-antibody-potentiates-the-effect-of-exogenous-surfactant-in-respiratory-failure-caused-by-meconium-aspiration
#18
Pavol Mikolka, Jana Kopincova, Petra Kosutova, Maros Kolomaznik, Andrea Calkovska, Daniela Mokra
AIM: Meconium aspiration syndrome (MAS) is life-threatening respiratory failure of newborns which can be treated by exogenous surfactant. In response to meconium, increased levels of chemokine IL-8 (CXCL8) stimulate massive neutrophil infiltration of the lungs. Local accumulation and activation of neutrophils, on-going inflammation, lung edema, and oxidative damage contribute to inactivation of endogenous and therapeutically given surfactants. Therefore, we have hypothesized that addition of monoclonal anti-IL-8 antibody into exogenous surfactant can mitigate the neutrophil-induced local injury and the secondary surfactant inactivation and may finally result in improvement of respiratory functions...
February 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29236548/budesonide-instillation-immediately-after-reperfusion-ameliorates-ischemia-reperfusion-induced-injury-in-the-transplanted-lung-of-rat
#19
Wei Gao, Qiu-Ming Meng, Xiao-Guang Cui
PURPOSE: Lung ischemia-reperfusion injury (LIRI) after lung transplantation can lead to primary graft dysfunction. Budesonide can improve endothelial function to reduce lung injury. This study was aimed to examine the effects of budesonide on LIRI and potential mechanisms. METHODS: Wistar rats were randomized and transplanted with syngeneic left lung or received the sham surgery. The recipients were instilled with saline or budesonide immediately after reperfusion...
December 13, 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/29252074/murine-model-of-oropharyngeal-gastric-fluid-aspiration-a-new-assessment-method-for-intrapulmonary-liquid-distribution-using-digital-pixel-calculation
#20
Dylan Beinart, Sade M B Finn, Uwe Scheuermann, Zoie E Holzknecht, Andrew S Barbas, William Parker, Shu S Lin
AIM OF THE STUDY: The aim of this study was to investigate a new method for visualization and quantification of intrapulmonary liquid distribution after oropharyngeal gastric fluid aspiration in mice. MATERIAL AND METHODS: Eleven mice received oropharyngeal aspiration with a gastric fluid, India ink, and saline solution. Digital imaging and pixel calculation were used to analyze intrapulmonary fluid distribution selectively. RESULTS: Digital pixel analysis and orophanryngeal aspiration are both safe techniques in mice and deliver reproducible/valid results...
November 2017: Experimental Lung Research
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