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Ultrastructural Pathology

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https://www.readbyqxmd.com/read/29192848/effects-of-resveratrol-on-high-fructose-induced-testis-injury-in-rats
#1
Elif Guzel Meydanli, Asli Gumusel, Serbay Ozkan, Gamze Tanriverdi, M B Can Balci, Seval Develi Is, A Ismet Hazar, Mujdat Uysal, Seldag Bekpinar
This study investigated whether a high-fructose (HFr) diet changes the morphology of seminiferous tubules (ST) in rats and resveratrol (RES) has a possible restoring effect in this sense. Fructose (30%; w/v) was administered to rats alone or together with RES (50 mg/L) in drinking water for 8 weeks. In the HFr group, destruction of the germinal epithelium led to the detection of immature germ cells in the lumen. HFr diet gave rise to a decrease in the ST diameters (p < 0.05), Johnsen's tubular biopsy score values (p < 0...
December 1, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29192847/acid-etching-technique-of-non-decalcified-bone-samples-for-visualizing-osteocyte-lacuno-canalicular-network-using-scanning-electron-microscope
#2
Tiina Lampi, Hannah Dekker, Chris M Ten Bruggenkate, Engelbert A J M Schulten, Jopi J W Mikkonen, Arto Koistinen, Arja M Kullaa
The aim of this study was to define the acid-etching technique for bone samples embedded in polymethyl metacrylate (PMMA) in order to visualize the osteocyte lacuno-canalicular network (LCN) for scanning electron microscopy (SEM). Human jaw bone tissue samples (N = 18) were collected from the study population consisting of patients having received dental implant surgery. After collection, the bone samples were fixed in 70% ethanol and non-decalcified samples embedded routinely into polymethyl metacrylate (PMMA)...
December 1, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29192845/systematic-alteration-of-apoptosis-a-review-with-ultrastructural-observations-on-leukemia-cells-in-vivo
#3
Yong-Xin Ru, Shi-Xuan Zhao, Shu-Xu Dong, Hao-Yue Liang, Ying Wang
The ultrastructural characteristics of apoptosis have been described microscopically for four decades. Alterations of nuclei, apoptotic bodies, cytoplasm, and some organelles have been illustrated and investigated during apoptosis. The successive changes of cellular components corresponding with differentiation of apoptotic cells are illustrated in the present review, based on ultrastructural observation of leukemia cells of patients in our routine clinic work by transmission electron microscopy. Most electron micrographs demonstrated that membranous components of nuclear envelop, rough endoplasmic reticulum and Golgi apparatus, and mitochondria were degenerated step by step during apoptosis...
December 1, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29192844/resveratrol-reduces-light-and-electron-microscopic-changes-in-acetaminophen-induced-hepatotoxicity-in-rats-role-of-inos-expression
#4
Hulya Elbe, Mehmet Gul, Asli Cetin, Elif Taslidere, Fatma Ozyalin, Yusuf Turkoz, Ali Otlu
INTRODUCTION: Hepatotoxicity is a major complication of acetaminophen (APAP), a widely used analgesic and antipyretic drug. Resveratrol (RSV) is a naturally occurring diphenol and it has anticancer, antioxidant, and anti-inflammatory properties. OBJECTIVES: In this study, the beneficial effects of RSV on APAP-induced hepatotoxicity was investigated in rats. MATERIALS AND METHODS: Group 1: Ethanol, Group 2: Saline, Group 3: RSV (10 mg/kg/ip), Group 4: APAP (1000 mg/kg/ip/single dose), Group 5: APAP+RSV (20 min after administration of APAP)...
December 1, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29192840/betulinic-acid-induces-apoptosis-and-ultrastructural-changes-in-mda-mb-231-breast-cancer-cells
#5
Yang Gao, Qing Ma, Yan-Bin Ma, Liang Ding, Xiao-Long Xu, De-Fei Wei, Lei Wei, Jing-Wei Zhang
The aim of this study is to investigate the effects of betulinic acid (BA) on triple-negative breast cancer MDA-MB-231 cells and observe the ultrastructural changes. The concentration of BA required to induce apoptosis in MDA-MB-231 cells has been previously reported. In this study, a cell counting kit-8 proliferation assay was used to measure cell viability and the apoptosis rate. Western blotting was performed to observe the protein expression levels of Bcl-2. Cell morphology and changes in cell density were observed by microscopy...
December 1, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29192811/effects-of-vitamin-d-on-ovary-in-dhea-treated-pcos-rat-model-a-light-and-electron-microscopic-study
#6
Latife Seyran Çelik, Yurdun Kuyucu, Ebru Dündar Yenilmez, Abdullah Tuli, Kenan Dağlıoğlu, Ufuk Özgü Mete
AIM: The aim of this study was to investigate the effects of vitamin D treatment on ovary in experimentally designed polycystic ovary syndrome of female rats using light and electron microscopic techniques. METHODS: Twenty-four female pre-pubertal rats were divided into control, DHEA and DHEA+Vit.D groups. In DHEA group, the PCOS rat model was developed by 6mg/kg/day dehydroepiandrosterone administration as subcutaneously injections. In DHEA+Vit.D group, 6 mg/kg/day DHEA and 120ng/100g/week 1,25(OH)2D3 was performed simultaneously...
December 1, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29192810/histological-and-ultrastructural-evaluation-of-human-decellularized-matrix-as-a-hernia-repair-device
#7
Martina Ghetti, Valentina Papa, Giovanni Deluca, Valeria Purpura, Paolo Ruscelli, Davide Melandri, Daniela Capirossi, Evandro Nigrisoli, Paola Minghetti, Elena Bondioli, Giovanna Cenacchi
Recently, interest has been increasing for human decellularized matrices, due to their ability to reduce numerous side effects related to hernia repair. To date, only animal studies investigated the biological interaction post-implant of human decellularized matrices for soft tissue repair. Therefore, the aim of this study was to evaluate the morphological response one year post implant of human decellularized matrix, through morphological analysis of human biopsies. The histological and ultrastructural results revealed a perfect cellular repopulation and neoangiogenesis, with minimal inflammatory response and a well-organized collagen matrix...
December 1, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29192809/kidney-carcinoid-tumor-histological-immunohistochemical-and-ultrastructural-features
#8
Xin Gu, Minyu Cheng, Guillermo A Herrera
Primary carcinoid tumor of the kidney is uncommon. Only limited morphologic studies have been reported in the literature. In general, renal carcinoid tumor reveals typical morphological features as present in carcinoid tumors in the other organs, i.e. growing as trabeculae intermixed with nests of monotonous cuboidal cells that show "salt and pepper" nuclear appearance. As reported in other organs, different morphological expressions may also present in renal carcinoid tumors, such as papillary, pseudoglandular or solid growth patterns and clear/eosinophilic cytoplasmic changes...
December 1, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29192807/autophagy-in-advanced-low-and-high-grade-tubular-adenocarcinomas-of-the-stomach-an-ultrastructural-investigation
#9
Rosario Alberto Caruso, Giuseppe Angelico, Eleonora Irato, Rosalba de Sarro, Giovanni Tuccari, Antonio Ieni
Autophagy represents a catabolic process in which cellular protein and organelles are engulfed into autophagosomes, digested in lysosomes and reutilized for the cellular metabolism. In neoplastic conditions, autophagy may act either as a tumour suppressor avoiding the accumulation of damaged proteins and organelles or as a mechanism of cell survival promoting the tumour growth. Although enhanced autophagy has been reported in hypoxic areas of solid tumors, there are only few ultrastructural reports concerning the relationships between autophagy and tumor grade...
December 1, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29297778/chronic-cholestasis-is-associated-with-hypogonadism-and-premature-ovarian-failure-in-adult-rats-cholestasis-causes-ovarian-hypogonadism
#10
Yomna I Mahmoud
Hypogonadism is a well-known complication in males with chronic liver diseases. However, the consequences of chronic hepatopathies on female reproductive capacities have received relatively little attention. The present study evaluates the effect of chronic obstructive jaundice on the ovary of adult cycling rats. Estrous cyclicity was monitored to check the functional status of the hypothalamic-pituitary-ovarian axis. Ovarian changes were assessed using histomorphometric, immunohistochemical, and ultrastructural techniques...
January 2018: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29130841/ultrastructural-pathology-joins-forces-with-the-society-for-ultrastructural-pathology
#11
Guillermo A Herrera
No abstract text is available yet for this article.
November 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28925796/should-transmission-electron-microscopy-and-ultrastructural-cilia-evaluation-remain-part-of-the-diagnostic-work-up-for-primary-ciliary-dyskinesia
#12
Claudius Werner, Panayiotis Kouis
The diagnostic work-up for primary ciliary dyskinesia (PCD) traditionally includes ciliary ultrastructural assessment using transmission electron microscopy (TEM). However, the identification of genetic variants of PCD that are missed by TEM, along with the development of novel diagnostic modalities for PCD that allow structural evaluation of cilia, such as immunofluorescence analysis and the increased availability of genetic testing, calls into questioning the contemporary role of TEM in the diagnostic work-up for PCD...
September 19, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28925789/motile-cilia-defects-in-diseases-other-than-primary-ciliary-dyskinesia-the-contemporary-diagnostic-and-research-role-for-transmission-electron-microscopy
#13
Hannah M Mitchison, Amelia Shoemark
Ultrastructural studies have underpinned the cell biological and clinical investigations of the varied roles of motile cilia in health and disease, with a long history since the 1950s. Recent developments from transmission electron microscopy (TEM; cryo-electron microscopy, electron tomography) have yielded higher resolution and fresh insights into the structure and function of these complex organelles. Microscopy in ciliated organisms, disease models, and in patients with ciliopathy diseases has dramatically expanded our understanding of the ubiquity, multisystem involvement, and importance of cilia in normal human development...
September 19, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28922056/secondary-defects-detected-by-transmission-electron-microscopy-in-primary-ciliary-dyskinesia-diagnostics
#14
Mellisa Dixon, Amelia Shoemark
Primary ciliary dyskinesia (PCD) is predominantly an autosomal recessively inherited condition that affects ~1 in 15,000 people. Diagnosis of PCD can be complex and is ordinarily based on the results of multiple investigations. These investigations include nasal nitric oxide, high-speed video microscopy, genotyping, and electron microscopy analysis of ciliary ultrastructure. A diagnosis is ultimately confirmed by the presence of a hallmark defect identified by transmission electron microscopy or biallelic variants in a known PCD gene...
September 18, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28922052/applications-of-emerging-transmission-electron-microscopy-technology-in-pcd-research-and-diagnosis
#15
Amelia Shoemark
Primary Ciliary Dyskinesia (PCD) is a heterogeneous genetic condition characterized by dysfunction of motile cilia. Patients suffer from chronic infection and inflammation of the upper and lower respiratory tract. Diagnosis of PCD is confirmed by identification of a hallmark defect of ciliary ultrastructure or by identification of biallelic pathogenic mutations in a known PCD gene. Since the first description of PCD in 1976, assessment of ciliary ultrastructure by transmission electron microscopy (TEM) has been central to diagnosis and research...
September 18, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28915070/value-of-transmission-electron-microscopy-for-primary-ciliary-dyskinesia-diagnosis-in-the-era-of-molecular-medicine-genetic-defects-with-normal-and-non-diagnostic-ciliary-ultrastructure
#16
Adam J Shapiro, Margaret W Leigh
Primary ciliary dyskinesia (PCD) is a genetic disorder causing chronic oto-sino-pulmonary disease. No single diagnostic test will detect all PCD cases. Transmission electron microscopy (TEM) of respiratory cilia was previously considered the gold standard diagnostic test for PCD, but 30% of all PCD cases have either normal ciliary ultrastructure or subtle changes which are non-diagnostic. These cases are identified through alternate diagnostic tests, including nasal nitric oxide measurement, high-speed videomicroscopy analysis, immunofluorescent staining of axonemal proteins, and/or mutation analysis of various PCD causing genes...
September 15, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28891733/application-of-laboratory-and-digital-techniques-for-visual-enhancement-during-the-ultrastructural-assessment-of-cilia
#17
Josef A Schroeder
Routine diagnostic electron microscopy of primary ciliary dyskinesia (PCD) is based on the findings of ultrastructural defects of axonemal components. Assessment of the typical abnormalities can be enhanced by improving the sample preservation status using tannic acid (TA) as additive in the biopsy fixation or processing steps. Another option is the implementation of computer-assisted image analysis tools. Advancements in high-resolution 3D visualization of the axonemal structure have been noted, with great potential for the future diagnosis of inherited cilia disorders...
September 11, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28872924/editorial
#18
K Kyriacou, Panayiotis Yiallouros
No abstract text is available yet for this article.
September 5, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28829237/comparative-ultrastructural-hepatic-alterations-induced-by-free-and-liposome-encapsulated-mefenamic-acid
#19
Qais Bashir Jarrar, Muhammad Nazrul Hakim, Manraj Singh Cheema, Zainul Amiruddin Zakaria
Mefenamic acid (MFA) is used as an anti-inflammatory, antinociceptive, and antipyretic agent for treatment of a wide range of pathological disorders. While the uncertainty of its safety and the poor oral bioavailability constitute the major limiting factors of its medical use, considerable efforts including liposomal encapsulation are needed to achieve maximum therapeutic advantages. The current work was conducted to investigate the ultrastructural alterations in the liver induced by free MFA and its liposomal preparation...
September 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28805487/state-of-the-art-nanopathological-diagnostics
#20
Luca Roncati, Giuseppe Barbolini
The nanopathological diagnostics (ND) is an ultra-specialized branch of pathological anatomy aimed to identify the nanoparticles of metallic, semimetallic, or nonmetallic elements in the inorganic particulate matter present inside pathological tissues, even on the nanometer scale. ND exploits an environmental scanning electron microscope, connected to an X-ray microprobe mounted on an energy-dispersive spectrometer. The searching of nanoparticles can be performed on paraffin-embedded material, omitting emissions of black overlay and plating procedures...
September 2017: Ultrastructural Pathology
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