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Immunobiology

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https://www.readbyqxmd.com/read/27916281/intratumoral-heterogeneity-of-macrophages-and-fibroblasts-in-breast-cancer-is-associated-with-the-morphological-diversity-of-tumor-cells-and-contributes-to-lymph-node-metastasis
#1
Lubov A Tashireva, Evgeny V Denisov, Tatiana S Gerashchenko, Daria N Pautova, Mikhail A Buldakov, Marina V Zavyalova, Julia Kzhyshkowska, Nadezhda V Cherdyntseva, Vladimir M Perelmuter
Recent studies have highlighted the heterogeneity of the tumor microenvironment (ME) and the importance of its analysis to the understanding of its impact on clinical outcomes. In this study, we aimed to analyze the intratumoral distribution of macrophages and fibroblasts in breast cancer (BC) based on the morphological diversity of tumor cells (tubular, alveolar, solid, trabecular and discrete structures) and the clinicopathological parameters of the disease. Thirty-six patients with invasive breast carcinoma of no special type were included in the study...
November 27, 2016: Immunobiology
https://www.readbyqxmd.com/read/27899210/comparative-analysis-of-the-internalization-of-the-macrophage-receptor-sialoadhesin-in-human-and-mouse-primary-macrophages-and-cell-lines
#2
Marjorie De Schryver, Annelies Leemans, Isabel Pintelon, Davie Cappoen, Louis Maes, Guy Caljon, Paul Cos, Peter L Delputte
Sialoadhesin (Sn) is a surface receptor expressed on resident macrophages with the ability to bind with sialic acids. During inflammation, an upregulation of Sn is observed. Upon binding of monoclonal antibodies to Sn, the receptor becomes internalized and this has been observed in multiple species. The latter characteristic, combined with the strong upregulation of Sn on inflammatory macrophages and the fact that Sn-positive macrophages contribute to certain inflammatory diseases, makes Sn an interesting entry portal for phenotype-modulating or cytotoxic drugs...
November 21, 2016: Immunobiology
https://www.readbyqxmd.com/read/27889311/structural-evaluation-of-a-nanobody-targeting-complement-receptor-vsig4-and-its-cross-reactivity
#3
Yurong Wen, Zhenlin Ouyang, Steve Schoonooghe, Siyu Luo, Patrick De Baetselier, Wuyuan Lu, Serge Muyldermans, Geert Raes, Fang Zheng
Vsig4 is a recently identified immune regulatory protein related to the B7 family with dual functionality: a negative regulator of T cell activation and a receptor for the complement components C3b and C3c. Here we present a structural evaluation of a nanobody, Nb119, against the extracellular IgV domain protein of both mouse and human recombinant Vsig4, which have a high degree of sequence identity. Although mouse and human Vsig4 bind to Nb119 with a 250 times difference in dissociation constants, the interaction results in a highly identical assembly with a RMSD of 0...
November 18, 2016: Immunobiology
https://www.readbyqxmd.com/read/27890297/digesting-the-role-of-bone-marrow-macrophages-on-hematopoiesis
#4
REVIEW
Esther Heideveld, Emile van den Akker
Tissue resident macrophages are found in various tissues like Langerhans cells in the skin or alveolar macrophages in the lung, and their main function is to regulate organ homeostasis. They have also been observed in the bone marrow and these cells in particular have been gaining importance in recent years as they are key players in hematopoiesis. However, as the characterization and classification of these putatively different bone marrow resident macrophages is far from established there is a need to generate an overview of tissue resident macrophages of the bone marrow...
November 17, 2016: Immunobiology
https://www.readbyqxmd.com/read/27887739/differences-in-the-modulation-of-reactive-species-lipid-bodies-cyclooxygenase-2-5-lipoxygenase-and-ppar-%C3%AE-in-cerebral-malaria-susceptible-and-resistant-mice
#5
Tatiana K S Borges, Érica A R Alves, Henda A R Vasconcelos, Fabiana P Carneiro, André M Nicola, Kelly G Magalhães, Maria Imaculada Muniz-Junqueira
Proinflammatory responses are associated with the severity of cerebral malaria. NO, H2O2, eicosanoid and PPAR-γ are involved in proinflammatory responses, but regulation of these factors is unclear in malaria. This work aimed to compare the expression of eicosanoid-forming-enzymes in cerebral malaria-susceptible CBA and C57BL/6 and -resistant BALB/c mice. Mice were infected with Plasmodium berghei ANKA, and the survival rates and parasitemia curves were assessed. On the sixth day post-infection, cyclooxygenase-2 and 5-lipoxygenase in brain sections were assessed by immunohistochemistry, and, NO, H2O2, lipid bodies, and PPAR-γ expression were assessed in peritoneal macrophages...
November 17, 2016: Immunobiology
https://www.readbyqxmd.com/read/27871781/crocin-inhibits-rankl-induced-osteoclast-formation-and-bone-resorption-by-suppressing-nf-%C3%AE%C2%BAb-signaling-pathway-activation
#6
Lijia Fu, Fang Pan, Yong Jiao
Crocin is a dietary compound with antioxidant and anti-inflammatory properties, but its effects on bone resorption have not been well characterized. Here we address this issue by examining the direct effects of crocin on osteoclast cells in vitro. Osteoclastogenesis was induced by RANKL (receptor activator of NF-κB ligand) in mouse bone marrow-derived macrophages in the absence or presence of crocin at various concentrations. Further, the bone resorption activity of mature osteoclast treated with crocin was assessed by pit assay...
November 15, 2016: Immunobiology
https://www.readbyqxmd.com/read/27876379/microrna-profiling-of-human-intermediate-monocytes
#7
Adam M Zawada, Lu Zhang, Insa E Emrich, Kyrill S Rogacev, Nicolas Krezdorn, Björn Rotter, Danilo Fliser, Yvan Devaux, Loems Ziegler-Heitbrock, Gunnar H Heine
Among the three human monocyte subsets, intermediate CD14++CD16+ monocytes have been characterized as particularly proinflammatory cells in experimental studies and as potential biomarkers of cardiovascular risk in clinical cohorts. To further substantiate the distinct role of intermediate monocytes within human monocyte heterogeneity, we assessed subset-specific expression of miRNAs as central epigenetic regulators of gene expression. We hypothesized that intermediate monocytes have a distinct miRNA profile compared to classical and non-classical monocytes...
November 13, 2016: Immunobiology
https://www.readbyqxmd.com/read/27843000/changes-in-gene-expression-induced-by-histamine-fexofenadine-and-osthole-expression-of-histamine-h1-receptor-cox-2-nf-%C3%AE%C2%BAb-ccr1-chemokine-ccl5-rantes-and-interleukin-1%C3%AE-in-pbmc-allergic-and-non-allergic-patients
#8
Natalia Karolina Kordulewska, Elżbieta Kostyra, Anna Cieślińska, Michał Matysiewicz, Ewa Fiedorowicz, Edyta Sienkiewicz-Szłapka
INTRODUCTION: Fexofenadine (FXF) is a third-generation antihistamine drug and osthole is assumed as a natural antihistamine alternative. This paper compares results of histamine, FXF and osthole impact on HRH-1, COX-2, NF-κB-p50, CCR1 mRNA expression. We also measured mRNA expression of IL-1β and CCL5/RANTES in incubated peripheral blood mononuclear cells (PBMC) to compared how histamine, FXF and osthole had influence on expression level and interacts on product secretion. OBJECTIVE: The purpose was to investigate expression pattern in asthma PBMC...
November 10, 2016: Immunobiology
https://www.readbyqxmd.com/read/27839836/different-antiviral-effects-of-ifn%C3%AE-and-ifn%C3%AE-in-an-hbv-mouse-model
#9
Yun Zhou, Sheng Li, Zongsheng Tang, Chunli Xu, Shunmei Huang, Jun Wu, Ulf Dittmer, Julia Dickow, Kathrin Sutter, Mengji Lu, Dongliang Yang, Jingjiao Song
Interferons α and β (IFNα and IFNβ) are type I interferons produced by the host to control pathogen propagation. However, only a minority of chronic hepatitis B (CHB) patients generate a sustained response after treatment with recombinant IFNα. The anti-HBV effect of IFNβ and the underlying mechanism are not well-understood. Here, we compared the antiviral activities of IFNα and IFNβ by application of IFNα or IFNβ expression plasmids using the well-established HBV hydrodynamic injection (HI) mouse model...
November 8, 2016: Immunobiology
https://www.readbyqxmd.com/read/27908642/negative-regulation-of-nlrp3-inflammasome-by-sirt1-in-vascular-endothelial-cells
#10
Yanxiang Li, Xiaofeng Yang, Yanhao He, Weirong Wang, Jiye Zhang, Wei Zhang, Ting Jing, Bo Wang, Rong Lin
NLRP3 inflammasome not only functions as a critical effector in innate immunity, but also triggers the production of proinflammatory cytokines involved in inflammation-associated diseases. Sirtuin 1 (SIRT1) plays an important role in the regulation of cellular inflammation. However, whether the activation of NLRP3 inflammasome is regulated by SIRT1 remains unknown. In this study, we investigated the regulatory effect of SIRT1 on NLRP3 inflammasome and the underlying mechanisms. We found that lipopolysaccharide (LPS) and adenosine triphosphate (ATP)-induced the activation of NLRP3 inflammasome in human umbilical vein endothelial cells (HUVECs)...
November 4, 2016: Immunobiology
https://www.readbyqxmd.com/read/27871782/association-of-high-hla-e-expression-during-acute-cellular-rejection-and-numbers-of-hla-class-i-leader-peptide-mismatches-with-reduced-renal-allograft-survival
#11
Hana Guberina, Vera Rebmann, Bettina Wagner, Fabiola da Silva Nardi, Phillip Dziallas, Sebastian Dolff, Anja Bienholz, Jeremias Wohlschlaeger, Agnes Bankfalvi, Falko M Heinemann, Oliver Witzke, Yvonne M Zoet, Frans H J Claas, Peter A Horn, Andreas Kribben, Ilias I N Doxiadis
Non-classical Human Leukocyte Antigen (HLA)-E preferentially presents leader peptides derived from classical HLA-class I molecules. HLA-E can trigger opposed immune responses by interacting with inhibitory NKG2A or by activating NKG2C receptors on NK and T-cells. We studied the impact of HLA-E on renal allograft survival during acute cellular rejection. HLA-E expression was up-regulated in acute cellular rejection (ACR) biopsies (n=12) compared to biopsies from 13 renal allografts with no rejection-signs. HLA-E up-regulation was correlated with numbers of HLA-class I leader peptide mismatches (p=0...
November 4, 2016: Immunobiology
https://www.readbyqxmd.com/read/27832933/a-plant-cell-expressed-recombinant-anti-tnf-fusion-protein-is-biologically-active-in-the-gut-and-alleviates-immune-mediated-hepatitis-and-colitis
#12
Yaron Ilan, Svetlana Gingis-Velitski, Ami Ben Ya'aco, Yehudit Shabbat, Lidya Zolotarov, Einat Almon, Yoseph Shaaltiel
: The orally administered BY-2 plant cell-expressed recombinant anti-TNF fusion protein (PRX-106) (n=6) consists of the soluble form of the human TNF receptor (TNFR) fused to the Fc component of a human antibody IgG1 domain. AIM: To evaluate the immune modulatory effect of the oral administration of plant cells expressing PRX-106. METHODS: Mice treated with Concanavalin A (ConA) to induce immune hepatitis was orally treated with cells expressing PRX-106 containing 0...
November 4, 2016: Immunobiology
https://www.readbyqxmd.com/read/27839837/exogenous-calreticulin-incorporated-onto-non-infective-trypanosoma-cruzi-epimastigotes-promotes-their-internalization-into-mammal-host-cells
#13
Eduardo Sosoniuk-Roche, Gerardo Vallejos, Lorena Aguilar-Guzmán, Javier Pizarro-Bäuerle, Katherine Weinberger, Carlos Rosas, Carolina Valck, Marek Michalak, Arturo Ferreira
Chagas disease is an endemic pathology in Latin America, now emerging in developed countries, caused by the intracellular protozoan Trypanosoma cruzi, whose life cycle involves three stages: amastigotes, epimastigotes, and trypomastigotes. T. cruzi Calreticulin (TcCRT), an endoplasmic reticulum resident chaperone, translocates to the external cellular membrane, where it captures complement component C1, ficolins and MBL, thus inactivating the classical and lectin pathways. Trypomastigote-bound C1 is detected as an "eat me" signal by macrophages and promotes the infective process...
October 29, 2016: Immunobiology
https://www.readbyqxmd.com/read/27817988/expression-and-characterization-of-recombinant-chicken-mannose-binding-lectin
#14
Weidong Zhang, Martin van Eijk, Hongbo Guo, Albert van Dijk, Onno B Bleijerveld, M Hélène Verheije, Guanbo Wang, Henk P Haagsman, Edwin J A Veldhuizen
Mannose binding lectin (MBL) is a serum collagenous C-type lectin that plays an important role in the innate immune protection against pathogens. Previously, human and mouse studies have demonstrated that MBL binds a broad range of pathogens that results in their neutralization through agglutination, enhanced phagocytosis, and/or complement activation via the lectin pathway. The role of MBL in chicken is not well understood although the MBL concentration in serum seems to correlate with protection against infections...
October 29, 2016: Immunobiology
https://www.readbyqxmd.com/read/27817989/heme-oxygenase-1-affects-granulopoiesis-in-mice-through-control-of-myelocyte-proliferation
#15
Karolina Bukowska-Strakova, Maciej Ciesla, Krzysztof Szade, Witold Norbert Nowak, Robert Straka, Agata Szade, Malgorzata Tyszka-Czochara, Karolina Najder, Anna Konturek, Maciej Siedlar, Jozef Dulak, Alicja Jozkowicz
Heme oxygenase-1 (HO-1) is stress-inducible, cytoprotective enzyme degrading heme to carbon monoxide (CO), biliverdin and Fe(2+). We showed that HO-1 knock-out mice (HO-1(-/-)) have a twofold higher level of granulocytes than wild type (WT) mice, despite decreased concentration of granulocyte colony-stimulating factor (G-CSF) in the blood and reduced surface expression of G-CSF receptor on the hematopoietic precursors. This suggests the effect of HO-1 on granulopoiesis. Here we aimed to determine the stage of granulopoiesis regulated by HO-1...
October 21, 2016: Immunobiology
https://www.readbyqxmd.com/read/27793398/novel-expression-of-a-functional-trimeric-fragment-of-human-sp-a-with-efficacy-in-neutralisation-of-rsv
#16
Alastair Watson, Nina Kronqvist, C Mirella Spalluto, Mark Griffiths, Karl J Staples, Tom Wilkinson, Uffe Holmskov, Grith L Sorensen, Anna Rising, Jan Johansson, Jens Madsen, Howard Clark
Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis and hospitalisation of infants in developed countries. Surfactant protein A (SP-A) is an important innate immune molecule, localized in pulmonary surfactant. SP-A binds to carbohydrates on the surface of pathogens in a calcium-dependent manner to enable neutralisation, agglutination and clearance of pathogens including RSV. SP-A forms trimeric units and further oligomerises through interactions between its N-terminal domains. Whilst a recombinant trimeric fragment of the closely related molecule (surfactant protein D) has been shown to retain many of the native protein's functions, the importance of the SP-A oligomeric structure in its interaction with RSV has not been determined...
October 18, 2016: Immunobiology
https://www.readbyqxmd.com/read/27773663/alterations-of-trim21-mrna-expression-during-monocyte-maturation
#17
Marzena Lenart, Magdalena Rutkowska-Zapała, Rafał Szatanek, Kazimierz Węglarczyk, Małgorzata Stec, Karolina Bukowska-Strakova, Anna Gruca, Jarosław Czyż, Maciej Siedlar
Tripartite motif-containing protein 21 (TRIM21) play a dual role in the cytoplasm of the cells where it facilitates destruction of some antibody-coated viruses and some bacteria, and initiates synthesis of proinflammatory cytokines. Macrophages and CD16(+) monocyte subset can particularly participate in a proinflammatory response caused by viral infection, however, the molecular mechanisms underlying these processes are not fully understood. The aim of this study was to determine the level of TRIM21-mRNA expression in monocyte subsets including: classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+)) and non-classical (CD14(+)CD16(++)) monocytes, as well as during in vitro differentiation of the isolated monocytes towards dendritic cells or macrophages...
October 18, 2016: Immunobiology
https://www.readbyqxmd.com/read/27773662/regulatory-t-cells-in-malignant-pleural-effusions-subsequent-to-lung-carcinoma-and-their-impact-on-the-course-of-the-disease
#18
J Budna, Ł Spychalski, M Kaczmarek, M Frydrychowicz, J Goździk-Spychalska, H Batura-Gabryel, J Sikora
Tumors exert suppressive effects on the host immune system and tumor progression can be linked to functional impairments of immune cells. Regulatory T cells (Treg) are a subpopulation of T lymphocytes and play a key role in suppressing immune responses against autoimmune diseases and cancer. The aim of the study was to investigate the prevalence of Treg in malignant and benign pleural effusions and to evaluate the relationship between Treg frequency and disease advance. Pleural effusions from 76 patients were subjected to a routine laboratory diagnosis and analyzed by conventional cytology...
October 18, 2016: Immunobiology
https://www.readbyqxmd.com/read/27773660/psoriatic-inflammation-causes-hepatic-inflammation-with-concomitant-dysregulation-in-hepatic-metabolism-via-il-17a-il-17-receptor-signaling-in-a-murine-model
#19
Naif O Al-Harbi, Ahmed Nadeem, Mohammed M Al-Harbi, Khairy M A Zoheir, Mushtaq A Ansari, Ahmed M El-Sherbeeny, Khalid M Alanazi, Moureq R Alotaibi, Sheikh F Ahmad
Psoriatic inflammation has been shown to be associated with cardiovascular dysfunction and systemic inflammation. Recently, psoriasis has also been linked to hepatic disorders, however underlying mechanism connecting the two are unknown. IL-17A being a central pro-inflammatory cytokine in the pathogenesis of psoriasis may be involved in hepatic inflammation through its receptor and downward signaling; however so far no study has investigated IL-17A related signaling in the liver during psoriasis in a murine model...
October 18, 2016: Immunobiology
https://www.readbyqxmd.com/read/27771173/complement-activation-inflammation-and-relative-adamts13-deficiency-in-secondary-thrombotic-microangiopathies
#20
Péter Farkas, Dorottya Csuka, Bálint Mikes, György Sinkovits, Marienn Réti, Endre Németh, Kristóf Rácz, Krisztina Madách, Mihály Gergely, Judit Demeter, Zoltán Prohászka
BACKGROUND: The secondary forms of hemolytic uremic syndrome/thrombotic thrombocytopenic purpura (secondary TMA) emerge as complications of coexisting diseases. OBJECTIVES: We hypothesized that secondary TMA could be characterized by the presence of relative ADAMTS13 deficiency and complement activation, and this relationship may have a prognostic value for outcome. PATIENTS AND METHODS: Fifty-three patients with thrombotic microangiopathy (TMA) and coexisting disease (such as malignancies, sepsis, heart surgery with extracorporeal circulation, solid organ transplantation, systemic autoimmune disorders), 41 patient controls, and 34 healthy controls were enrolled in our case-control study with 30days follow-up...
October 18, 2016: Immunobiology
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