journal
MENU ▼
Read by QxMD icon Read
search

Immunobiology

journal
https://www.readbyqxmd.com/read/28526484/regulation-of-cacybp-sip-expression-by-nfat1-transcription-factor
#1
Beata Kądziołka, Wiesława Leśniak, Anna Filipek
In this work we have shown that NFAT1 transcription factor is involved in the regulation of CacyBP/SIP expression. We have demonstrated, by applying Western blot, RT-PCR and luciferase assay that the level of CacyBP/SIP increases upon NFAT1 overexpression. Moreover, inhibition or stimulation of NFAT transcriptional activity exerts a corresponding effect on the expression of CacyBP/SIP gene. Furthermore, EMSA and chromatin immunoprecipitation (ChIP) assay have shown that NFAT1 binds to its specific binding sites within the CacyBP/SIP gene...
May 11, 2017: Immunobiology
https://www.readbyqxmd.com/read/28363498/t-cell-metabolism-in-metabolic-disease-associated-autoimmunity
#2
REVIEW
Jacob Amersfoort, Johan Kuiper
This review discusses the relevant metabolic pathways and their regulators which show potential for T cell metabolism-based immunotherapy in diseases hallmarked by both metabolic disease and autoimmunity. Multiple therapeutic approaches using existing pharmaceuticals are possible from a rationale in which T cell metabolism forms the hub in dampening the T cell component of autoimmunity in metabolic diseases. Future research into the effects of a metabolically aberrant micro-environment on T cell metabolism and its potential as a therapeutic target for immunomodulation could lead to novel treatment strategies for metabolic disease-associated autoimmunity...
March 23, 2017: Immunobiology
https://www.readbyqxmd.com/read/28390705/the-protection-role-of-atg16l1-in-cd11c-dendritic-cells-in-murine-colitis
#3
Hong Zhang, Libo Zheng, Jeremy Chen, Masayuki Fukata, Ryan Ichikawa, David Q Shih, Xiaolan Zhang
The autophagy-related 16-like 1 gene (Atg16l1) is associated with inflammatory bowel disease (IBD) and has been shown to play an essential role in paneth cell function and intestinal homeostasis. However, the functional consequences of Atg16l1 deficiency in myeloid cells, particularly in dendritic cells (DCs), are not fully characterized. The aim of this study is to investigate the functional consequence of Atg16l1 in CD11c(+)DCs in murine colitis. We generated mice deficient in Atg16l1 in CD11c(+)DCs. Dextran Sulfate Sodium (DSS) and S...
March 16, 2017: Immunobiology
https://www.readbyqxmd.com/read/28356195/age-related-changes-and-distribution-of-t-cell-markers-cd3-and-cd4-and-toll-like-receptors-tlr2-tlr3-tlr4-and-tlr7-in-the-duck-lymphoid-organs
#4
Aiguo Zhang, Jiahua Xu, Hanzhang Lai, Wenke Huang, Niran Fang, Ruiai Chen
T lymphocytes and Toll-like receptors have been confirmed to have correlation with the ability to resistance to pathogenic challenges and play an important role in duck immune system. However, the information of ontogeny of T lymphocytes and Toll-like receptors is scarcely in duck. Therefore, to address these questions, we report the development and distribution of CD3 and CD4 by immunocytochemistry and the age-related mRNA level of duck T cell markers (CD3 and CD4) and Toll-like receptors (TLR2, TLR3, TLR4 and TLR7) by real time quantitative PCR in duck lymphoid organs (thymus, bursa of Fabricius and spleen)...
March 10, 2017: Immunobiology
https://www.readbyqxmd.com/read/28318800/autoimmune-disease-development-based-on-possible-mislocalization-of-intracellular-and-extracellular-proteins
#5
Borros Arneth
BACKGROUND: T helper cells can differentiate into several subsets of T lymphocytes, including Th1, Th2, and regulatory T (Treg) cells. As a result of this ability to differentiate, the corresponding T cell receptor (TCR) spectra display considerable cellular plasticity and interchangeability. In contrast, T lymphocyte differentiation and separation into CD4(+) and/or CD8(+) T cell lines creates stable populations over a person's lifetime, which abrogates the plasticity and interchange between these cell types and their corresponding TCR spectra but results in considerable stability regarding the corresponding TCR sequences and spectra...
February 27, 2017: Immunobiology
https://www.readbyqxmd.com/read/28012584/severe-disease-in-cystic-fibrosis-and-fecal-calprotectin-levels
#6
Giuseppe Fabio Parisi, Maria Papale, Novella Rotolo, Donatella Aloisio, Lucia Tardino, Maria Grazia Scuderi, Vincenzo Di Benedetto, Raffaella Nenna, Fabio Midulla, Salvatore Leonardi
Fecal calprotectin (FC) is used to asses the presence of intestinal inflammation also in patients with Cystic Fibrosis (CF) and recent studies showed a correlation between bowel and lung disease in these patients. The aim of this study was to analyze the levels of FC in CF and correlate them with different phenotypes of disease. We enrolled a cohort of 54 CF patients and 50 healthy controls. In these patients, calprotectin has been assayed on a stools sample using an ELISA kit. In all patients we analyzed, FC levels were elevated above the cut-off value and significantly higher than in healthy controls...
March 2017: Immunobiology
https://www.readbyqxmd.com/read/27780588/the-non-mammalian-mif-superfamily
#7
REVIEW
Amanda Sparkes, Patrick De Baetselier, Kim Roelants, Carl De Trez, Stefan Magez, Jo A Van Ginderachter, Geert Raes, Richard Bucala, Benoît Stijlemans
Macrophage migration inhibitory factor (MIF) was first described as a cytokine 50 years ago, and emerged in mammals as a pleiotropic protein with pro-inflammatory, chemotactic, and growth-promoting activities. In addition, MIF has gained substantial attention as a pivotal upstream mediator of innate and adaptive immune responses and with pathologic roles in several diseases. Of less importance in mammals is an intrinsic but non-physiologic enzymatic activity that points to MIF's evolution from an ancient defense molecule...
March 2017: Immunobiology
https://www.readbyqxmd.com/read/27769564/in-vivo-anti-inflammatory-activities-of-novel-cytokine-il-38-in-murphy-roths-large-mrl-lpr-mice
#8
Man Chu, Lai Shan Tam, Jing Zhu, Delong Jiao, De Hua Liu, Zhe Cai, Jie Dong, Christopher Wei Kai Lam, Chun Kwok Wong
The newly named interleukin (IL)-36 subfamily member IL-38 has been shown to exert anti-inflammatory activity. However, the in vivo immunomodulatory activity of IL-38 was poorly investigated in systemic lupus erythematosus (SLE). We have investigated the expression of CD4(+)IL-17(+) Th17, CD4(+)IFN-γ(+) Th1 and CD3(+)CD4(-)CD8(-) double negative (DN) T cells and the related immunopathological mechanisms in female MRL/lpr mice model of spontaneous lupus-like disease, with or without IL-38 treatment. Intravenous administration of murine recombinant IL-38 into MRL/lpr mice can ameliorate the lupus-like clinical symptoms including proteinuria, leukocyteuria and skin lesions...
March 2017: Immunobiology
https://www.readbyqxmd.com/read/28318799/transcriptome-analysis-of-il-10-stimulated-m2c-macrophages-by-next-generation-sequencing
#9
Emily B Lurier, Donald Dalton, Will Dampier, Pichai Raman, Sina Nassiri, Nicole M Ferraro, Ramakrishan Rajagopalan, Mahdi Sarmady, Kara L Spiller
Alternatively activated "M2" macrophages are believed to function during late stages of wound healing, behaving in an anti-inflammatory manner to mediate the resolution of the pro-inflammatory response caused by "M1" macrophages. However, the differences between two main subtypes of M2 macrophages, namely interleukin-4 (IL-4)-stimulated "M2a" macrophages and IL-10-stimulated "M2c" macrophages, are not well understood. M2a macrophages are characterized by their ability to inhibit inflammation and contribute to the stabilization of angiogenesis...
February 20, 2017: Immunobiology
https://www.readbyqxmd.com/read/28233577/tnf%C3%AE-induced-downregulation-of-microrna-186-contributes-to-apoptosis-in-rat-primary-cardiomyocytes
#10
Hua Xu, Jingyao Li, Yue Zhao, Dayi Liu
Progressive loss of cardiac cardiomyocytes is involved in pathogenesis of heart failure. Inflammation is considered as a major risk factor that triggers cardiomyocytes apoptosis or induces cellular damage. Proinflammatory cytokines such as TNFα can directly activate cell apoptosis or promote oxidant production that damages cellular structure eventually. We investigated TNFα mediated apoptosis in cultured rat primary cardiomyocytes. Annexin V/PI staining and apoptosis biomarker expression were used to examine cardiomyocytes cell apoptosis response...
February 16, 2017: Immunobiology
https://www.readbyqxmd.com/read/28237664/il-10-deficient-mice-express-ifn-%C3%AE-mrna-and-clear-leptospira-interrogans-from-their-kidneys-more-rapidly-than-normal-c57bl-6-mice
#11
Amy A Devlin, Priya J Halvorsen, Jennifer C Miller, Scott M Laster
Leptospira interrogans (L. interrogans), the causative agent of leptospirosis, is a widespread zoonotic spirochete that lives a dual lifestyle. L. interrogans infects mice, rats, and wildlife in a persistent and asymptomatic fashion, while also causing productive and acute infections in other mammals such as humans and hamsters. Infections in humans can be fatal, accompanied by a cytokine storm and shock-like symptoms. Production of IL-10 has been noted in both rodent and human infections which has led a number of investigators to hypothesize that IL-10 plays a role in the pathogenesis of this disease...
February 10, 2017: Immunobiology
https://www.readbyqxmd.com/read/28209270/crosstalk-between-glucocorticoids-and-il-4-modulates-ym1-expression-in-alternatively-activated-myeloid-cells
#12
Nathalie Ng Kuet Leong, Frank Brombacher, Alexander H Dalpke, Michael Weitnauer
Airway epithelial cells induce a tolerogenic microenvironment by modulating immune cells in the lung. We recently showed that the supernatant of airway epithelial cells induces two marker genes of alternative activation, Ym1 and Ms4a8a, in respiratory myeloid cells. This induction was partially mediated by glucocorticoids, secreted by airway epithelial cells. In this study, we further investigated Ym1 and Ms4a8a regulation in alternatively activated myeloid cells in the presence of the TH2 cytokines IL-4 and IL-13...
February 9, 2017: Immunobiology
https://www.readbyqxmd.com/read/28190533/the-roles-of-a-ribosomal-protein-s19-polymer-in-a-mouse-model-of-carrageenan-induced-acute-pleurisy
#13
Koji Yamanegi, Toru Kawakami, Naoko Yamada, Shunsuke Kumanishi, Hiroyuki Futani, Keiji Nakasho, Hiroshi Nishiura
C5-deficient mice usually present moderate neutrophil activation during the initiation phase of acute inflammation. Conversely, C5a receptor (C5aR)-deficient mice show unusually excessive activation of neutrophils. We identified the ribosomal protein S19 (RP S19) polymer, which is cross-linked at Lys122 and Gln137 by transglutaminases in apoptotic neutrophils, as a second C5aR ligand during the resolution phase of acute inflammation. The RP S19 polymer promotes apoptosis via the neutrophil C5aR and phagocytosis via the macrophage C5aR...
February 7, 2017: Immunobiology
https://www.readbyqxmd.com/read/28189340/molecular-identification-and-functional-characterization-of-a-tumor-necrosis-factor-tnf-gene-in-crassostrea-hongkongensis
#14
Fufa Qu, Zhiming Xiang, Yang Zhang, Jun Li, Shu Xiao, Yuehuan Zhang, Yanping Qin, Yingli Zhou, Ziniu Yu
Tumor necrosis factor superfamily (TNFSF) represents a group of multifunctional inflammatory cytokines that have been shown to participate in a variety of pathological and immunological process. However, the functions of these proteins in oyster are still poorly understood. In the present study, an oyster TNF homolog (named ChTNF) was identified from a cDNA library of Crassostrea hongkongensis. The complete cDNA of ChTNF was 2457bp in length containing an open reading frame (ORF) of 1044bp, a 5'-untranslated region (UTR) of 381bp and a 3'-UTR of 1032bp...
February 6, 2017: Immunobiology
https://www.readbyqxmd.com/read/28164808/emerging-food-allergens-identification-of-polyphenol-oxidase-as-an-important-allergen-in-eggplant-solanum-melongena-l
#15
Bheemanapalli N Harish Babu, Anthony Wilfred, Yeldur P Venkatesh
Although many allergens have been detected in eggplant (Solanum melongena L.), their identity have not been elucidated. The aim of this study was to investigate whether polyphenol oxidase (PPO), an important eggplant enzyme, acts as an allergen. The proteins of eggplant peel extract were separated on phenyl-Sepharose (PS), and analyzed by skin prick test (SPT), ELISA and IgE-immunoblotting; the components were analyzed for PPO activity, presence of protein-bound copper, and recognition by rabbit polyclonal anti-sweet potato PPO antiserum...
February 2017: Immunobiology
https://www.readbyqxmd.com/read/28164807/induction-of-b7-h1-receptor-by-bacterial-cells-fractions-of-porphyromonas-gingivalis-on-human-oral-epithelial-cells-b7-h1-induction-by-porphyromonas-gingivalis-fractions
#16
S Groeger, F Jarzina, U Mamat, J Meyle
The immune-regulatory B7-H1 receptor, also known as programmed death-ligand 1 (PD-L1), plays an important role in cell-mediated immune response. It is a co-signaling molecule that mediates regulation of T cell activation and tolerance and is able to negatively regulate activated T cell functions and survival. High expression of B7-H1 in host cells may contribute to the chronicity of inflammatory disorders and represents a possible mechanism of immune evasion. Porphyromonas gingivalis is regarded as a keystone pathogen in periodontitis and is able to invade host cells and disposes a variety of virulence factors including lipopolysaccharide (LPS), fimbriae and proteases such as gingipains...
February 2017: Immunobiology
https://www.readbyqxmd.com/read/27793398/novel-expression-of-a-functional-trimeric-fragment-of-human-sp-a-with-efficacy-in-neutralisation-of-rsv
#17
Alastair Watson, Nina Kronqvist, C Mirella Spalluto, Mark Griffiths, Karl J Staples, Tom Wilkinson, Uffe Holmskov, Grith L Sorensen, Anna Rising, Jan Johansson, Jens Madsen, Howard Clark
Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis and hospitalisation of infants in developed countries. Surfactant protein A (SP-A) is an important innate immune molecule, localized in pulmonary surfactant. SP-A binds to carbohydrates on the surface of pathogens in a calcium-dependent manner to enable neutralisation, agglutination and clearance of pathogens including RSV. SP-A forms trimeric units and further oligomerises through interactions between its N-terminal domains. Whilst a recombinant trimeric fragment of the closely related molecule (surfactant protein D) has been shown to retain many of the native protein's functions, the importance of the SP-A oligomeric structure in its interaction with RSV has not been determined...
February 2017: Immunobiology
https://www.readbyqxmd.com/read/27773661/insights-into-the-interaction-of-cd4-with-anti-cd4-antibodies
#18
Van-Chien Bui, Thi-Huong Nguyen
Knowledge about the mechanism by which some antibodies can block HIV-1 entry is critical to our understanding of their function and may offer new avenues for controlling the adhesion of HIV-1 to the host cells. While much progress has been made, this mechanism remains unclear. Here, atomic force microscopy, isothermal titration calorimetry (ITC), and circular dichroism spectroscopy were used to measure some biophysical characteristics of the interaction of four-domains (D1-D4) membrane protein CD4 with anti-D3 antibody OKT4 and with HIV-1 entry blocking anti-D1 antibody Leu3a...
February 2017: Immunobiology
https://www.readbyqxmd.com/read/27773660/psoriatic-inflammation-causes-hepatic-inflammation-with-concomitant-dysregulation-in-hepatic-metabolism-via-il-17a-il-17-receptor-signaling-in-a-murine-model
#19
Naif O Al-Harbi, Ahmed Nadeem, Mohammed M Al-Harbi, Khairy M A Zoheir, Mushtaq A Ansari, Ahmed M El-Sherbeeny, Khalid M Alanazi, Moureq R Alotaibi, Sheikh F Ahmad
Psoriatic inflammation has been shown to be associated with cardiovascular dysfunction and systemic inflammation. Recently, psoriasis has also been linked to hepatic disorders, however underlying mechanism connecting the two are unknown. IL-17A being a central pro-inflammatory cytokine in the pathogenesis of psoriasis may be involved in hepatic inflammation through its receptor and downward signaling; however so far no study has investigated IL-17A related signaling in the liver during psoriasis in a murine model...
February 2017: Immunobiology
https://www.readbyqxmd.com/read/27771174/down-regulation-of-na-h-exchanger-1-by-toll-like-receptor-stimulation-in-macrophages
#20
Shiho Takakuwa, Natsumi Mizuno, Takayuki Takano, Sanki Asakawa, Taiki Sato, Masahiro Hiratsuka, Noriyasu Hirasawa
The role of Na(+)/H(+) exchanger 1 (NHE1) in various cell types, including inflammatory cells, has been extensively studied. However, regulation of NHE1 protein level in activated inflammatory cells is yet to be characterized. In this study, we investigated whether Toll-like receptor (TLR) ligands can regulate NHE1 protein level in the mouse macrophage-like RAW 264 cell line. We found that lipopolysaccharide (LPS), a TLR4 ligand, lowered NHE1 level and activity in RAW 264 cells and in primary murine macrophages...
February 2017: Immunobiology
journal
journal
27872
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"