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International Journal of Biological Macromolecules

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https://www.readbyqxmd.com/read/28919532/performance-of-cellulose-acetate-ferric-oxide-nanocomposite-supported-metal-catalysts-toward-the-reduction-of-environmental-pollutants
#1
Esraa M Bakhsh, Shahid Ali Khan, Hadi M Marwani, Ekram Y Danish, Abdullah M Asiri, Sher Bahadar Khan
Water contamination by toxic compounds has become one of the most serious problems worldwide. Catalytic reduction using metal nanoparticles offer opportunities for environmental benefits. In this study, cellulose acetate-ferric oxide nanocomposite (CA/Fe2O3) was prepared and used as support for metal nanoparticles. After adsorption of Ag, Cu or Ni ions from aqueous solutions, metal ions associated with CA/Fe2O3 were treated with sodium borohydride to prepare Ag, Cu and Ni nanoparticles loaded CA/Fe2O3. The CA/Fe2O3 supported Ag, Cu or Ni nanoparticles was evaluated as a catalyst for pollutants degradation...
September 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28919531/a-thermostable-alkaliphilic-protein-disulfide-isomerase-from-bacillus-subtilis-dr8806-cloning-expression-biochemical-characterization-and-molecular-dynamics-simulation
#2
Milad Lagzian, Ali Shahraki, Mahdiyeh Besharatian, Ahmad Asoodeh
Disulfide bonds are among the most important factors related to correct folding of the proteins. Protein disulfide isomerase (PDI) is the enzyme responsible for the correct formation and isomerization of these bonds. It is rarely studied so far and none of them showed industrial properties. In this study, the gene encoding for a putative PDI from Bacillus subtilis DR8806 was identified, cloned and expressed in Escherichia coli. It was encoded a 23.26kDa protein. The enzyme was purified by GST affinity chromatography with a specific activity of 1227u/mg...
September 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28919530/coproduction-of-protease-and-mannanase-from-bacillus-nealsonii-pn-11-in-solid-state-fermentation-and-their-combined-application-as-detergent-additives
#3
Aditi David, Aditya Kumar, Steffy Angural, Deepak Kumar, Neena Puri, Naveen Gupta
Bacillus nealsonii PN-11 produces thermo-alkalistable mannanase and protease active in wide temperature and pH range. Optimization of coproduction of protease and mannanase from this strain and application of cocktail of these enzymes as detergent additives were studied. On optimization mannanase yield of 834Ug(-1) (11.12 fold increase) and protease yield of 70Ug(-1) (4.7 fold increase) could be obtained in a single fermentation. Purification and characterization of mannanase have been done earlier and protease was done during this study and has a molecular mass of 48kDa...
September 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28919529/acetylation-of-lysine-residues-in-apomyoglobin-structural-changes-amyloid-fibrillation-and-role-of-surface-charge
#4
Mehrnaz Azami-Movahed, Ali Akbar Meratan, Atiyeh Ghasemi, Azadeh Ebrahim-Habibi, Mohsen Nemat-Gorgani
Post-translational modifications play important roles in conformational properties and aggregation propensities of different peptides and proteins. In the present study, we have investigated the effects of acetylation of lysine residues on the structure and aggregation properties of apomyoglobin (apoMb).All of the 19 lysine residues were modified. Far-, near-UV CD, intrinsic and acrylamide quenching fluorescence studies indicated that acetylation significantly influences conformation of apoMb by altering both its secondary and tertiary structures...
September 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28919528/modification-of-wool-protein-fiber-with-plasma-and-dendrimer-effects-on-dyeing-with-cochineal
#5
Toktam Sajed, Aminoddin Haji, Mohammad Khajeh Mehrizi, Majid Nasiri Boroumand
In this study, plasma treatment and a poly(propylene imine) dendrimer were employed to improve the dyeability of wool fibers with cochineal natural dye. FESEM, EDX, AFM and FTIR techniques were employed to investigate the effects of these treatments on chemical and physical properties of wool fibers. The etching of surface layer of wool fibers and increased roughness after plasma treatment was confirmed by FESEM and AFM images. EDX and FTIR analyses confirmed the creation of oxygen-containing groups and attachment of dendrimer molecules on wool fibers after plasma and dendrimer treatments respectively...
September 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28919527/protective-effect-of-porcine-plasma-protein-hydrolysates-on-the-gelation-of-porcine-myofibrillar-protein-exposed-to-a-hydroxyl-radical-generating-system
#6
Haili Niu, Yichun Chen, Huan Zhang, Baohua Kong, Qian Liu
This study investigated the effect of different concentrations of porcine plasma protein hydrolysates (PPPH) on the gelation of porcine myofibrillar protein (MP) exposed to a hydroxyl radical-generating system (HRGS). Compared to non-oxidized MP, the oxidative modification led to a decreased penetration force and water holding capacity (WHC) (P<0.05). Meanwhile, addition of PPPH (1.5mg/mL) reduced the loss of MP gel strength and WHC induced by HRGS (P<0.05). Low-field nuclear magnetic resonance results suggested that the addition of PPPH facilitated the hydration of the protein...
September 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28919526/chemical-crosslinking-of-biopolymeric-scaffolds-current-knowledge-and-future-directions-of-crosslinked-engineered-bone-scaffolds
#7
REVIEW
Ahmad Oryan, Amir Kamali, Ali Moshiri, Hossien Baharvand, Hamed Daemi
Bone tissue scaffolds made from either natural or synthetic polymers are employed to promote bone healing. However, lack of sufficient or poor mechanical properties such as low integrity and stability reduces their medical applications. Crosslinking, defined as induction of chemical or physical links among polymer chains, is a simple method generally used to modify mechanical, biological and degradation properties of hydrogels. Although crosslinking through chemical reactions improves the mechanical properties of bone substitutes, most of the reagents used for this aim demonstrate undesirable effects and may exert toxic reactions...
September 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28919525/gelatin-stabilization-of-quantum-dots-for-improved-stability-and-biocompatibility
#8
Sundararajan Parani, Kannaiyan Pandian, Oluwatobi Samuel Oluwafemi
We herein report an aqueous synthesis of gelatin stabilized CdTe/CdS/ZnS (CSSG) core/double shell quantum dots (QDs) with improved biocompatibility. The as-synthesized QDs were characterized by ultraviolet-visible (UV-Vis) and photoluminescence (PL) spectroscopic techniques, x-ray diffraction technique (XRD), x-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). The CSSG QDs revealed high photoluminescence quantum yield (PLQY) with excellent stability over a period of one year and retained 90% of its initial PLQY without any aggregation or precipitation under ambient condition...
September 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28919524/chitosan-ferulic-acid-coated-poly-%C3%AE%C2%B5-caprolactone-electrospun-materials-with-antioxidant-antibacterial-and-antitumor-properties
#9
Gyuldzhan Yakub, Milena Ignatova, Nevena Manolova, Iliya Rashkov, Reneta Toshkova, Ani Georgieva, Nadya Markova
Novel fibrous materials from poly(ε-caprolactone) (PCL), chitosan (Ch) and natural phenolic acid ferulic acid (FA) of diverse design were successfully prepared by electrospinning or electrospinning combined with dip-coating. FA incorporated in the PCL fibrous mats or in the Ch coating was in the amorphous state as evidenced by the performed differential scanning calorimetry (DSC) and X-ray diffraction (XRD) analysis. The release of FA was affected by the composition and design of the polymer matrix. The incorporation of a combination of FA and Ch in the fibrous mats imparted to these materials higher killing rates against pathogenic bacteria S...
September 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28917941/skin-regeneration-effect-of-the-glycosaminoglycans-from-liparis-tessellatus-eggs
#10
Jae-Joon Lee, Bernadeth F Ticar, Zuliyati Rohmah, Si-Hyang Park, Dawon Kang, Seok-Joong Kang, Byeong-Dae Choi
A purified Glycosaminoglycans (GAGs) extract from L. tessellatus eggs was enzyme-hydrolyzed and then fractionated with DEAE-Sepharose column chromatography. The fractions were subsequently subjected to skin regeneration effects analysis against skin fibroblast (CCD-986sk) cell lines. Fraction 3 is evidently to have tyrosinase inhibition activity by 20.1% at concentration of 50mg/mL. DOPA oxidation assay, collagenase inhibition activity assay, fibroblast proliferation assay, and production of type I C-peptide assays were done to further proof the skin regeneration effect of GAGs fractions...
September 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28917940/natural-and-synthetic-polymers-bioceramics-bioactive-compounds-mediated-cell-signaling-in-bone-tissue-engineering
#11
REVIEW
S Harsha Rao, B Harini, R Pranav Kumar Shadamarshan, K Balagangadharan, N Selvamurugan
Bone is a highly integrative and dynamic tissue of the human body. It is continually remodeled by bone cells such as osteoblasts, osteoclasts. When a fraction of a bone is damaged or deformed, stem cells and bone cells under the influence of several signaling pathways regulate bone regeneration at the particular locale. Effective therapies for bone defects can be met via bone tissue engineering which employs drug delivery systems with biomaterials to enhance cellular functions by acting on signaling pathways such as Wnt, BMP, TGF-β, and Notch...
September 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28917939/preparation-and-characterization-of-gelatin-%C3%AE-tcp-sf-biocomposite-scaffold-for-bone-tissue-regeneration
#12
JunTae Huh, JiUn Lee, WonJin Kim, Miji Yeo, GeunHyung Kim
In this study, we suggest a new biocomposite scaffold composed of gelatin/α-TCP (tricalcium phosphate)/SF (silk-fibroin) (GTS) which has enhanced mechanical strength and high level of cellular activity. To fabricate GTS scaffold, a temperature-controlled 3D printing process was used and appropriate printing conditions were selected based on rheological data. To show the feasibility as a biomedical scaffold for bone tissue regeneration, the various physical and biological results, using MG63 (osteoblast-like cells), of the GTS scaffold were compared with those of a pure gelatin (G) and gelatin/α-TCP (GT) composite scaffold...
September 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28917938/the-evaluation-of-antioxidant-and-antifungal-properties-of-6-amino-6-deoxychitosan-in-vitro
#13
Fang Luan, Qing Li, Wenqiang Tan, Lijie Wei, Jingjing Zhang, Fang Dong, Guodong Gu, Zhanyong Guo
We synthesized 6-amino-6-deoxychitosan (NCS) through a series of reactions from chitosan (CS). The antioxidant ability of CS and NCS were investigated in vitro, including that of DPPH-radical and hydrogen peroxide, reducing power, chelating abilities and inhibition of lipid peroxidation. As expected, after the introduction of amino groups, antioxidant ability had improved significantly. Especially, scavenging effect against DPPH-radical and hydrogen peroxide of NCS were 97% and 92% at 1.6mg/mL, respectively...
September 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28917937/pyrazole-based-compounds-in-chitosan-liposomal-emulsion-for-antimicrobial-cotton-fabrics
#14
Ahmed Nada, Marwa Al-Moghazy, Ahmed A F Soliman, Gehan M T Rashwan, Taghreed Hosny Ahmed Eldawy, Ashraf Abd Elhakim Hasan, Galal Hosni Sayed
The chemistry of pyrazoles has gained increasing attention due to its diverse pharmacological properties such as antiviral, antagonist, antimicrobial, anticancer, anti-inflammatory, analgesic, anti-prostate cancer, herbicidal, acaricidal and insecticidal activities. 1-phenyl pyrazole-3, 5-diamine, 4-[2-(4-methylphenyl) diazenyl] and 1H- pyrazole-3 (1), 5-diamine, 4-[2-(4-methylphenyl) diazenyl] (2) were synthesized, characterized and encapsulated into liposomal chitosan emulsions for textile finishing. The chemical modifications of cotton fabrics were demonstrated by infrared analysis...
September 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28916383/heterologous-expression-and-structure-function-relationship-of-low-temperature-and-alkaline-active-protease-from-acinetobacter-sp-ihb-b-5011-mn12
#15
Richa Salwan, Vivek Sharma, Mohinder Pal, Ramesh Chand Kasana, Sudesh Kumar Yadav, Arvind Gulati
The gene encoding protease from Acinetobacter sp. IHB B 5011(MN12) was cloned and expressed in Escherichia coli BL21(DE3). The nucleotide sequence revealed 1323bp ORF encoding 441 amino acids protein with molecular weight 47.2kDa. The phylogenetic analysis showed clustering of Alp protease with subtilisin-like serine proteases of S8 family. The amino acid sequence was comprised of N-terminal signal peptide 1-21 amino acids, pre-peptide 22-143 amino acids, peptidase S8 domain 144-434 amino acids, and pro-peptide 435-441 amino acids at C-terminus...
September 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28916382/influence-of-%C3%AE-lactoglobulin-and-calcium-chloride-on-the-molecular-structure-and-interactions-of-casein-micelles
#16
Yu Zhuang, Ikuko Ueda, Ulrich Kulozik, Ronald Gebhardt
Targeted processing of casein micelles (CM) requires a basic understanding of their molecular structure as well as their interactions with each other and with other components. In this study, angle- and concentration-dependent static and dynamic light scattering is applied to investigate changes in the molecular weight, size, and intermolecular interactions of CM after the addition of β-lactoglobulin (ß-Lg) and calcium chloride. Addition of a surplus of ß-Lg impairs the colloidal stability of CM. In the presence of 0...
September 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28916381/perspectives-on-the-production-structural-characteristics-and-potential-applications-of-bioplastics-derived-from-polyhydroxyalkanoates
#17
REVIEW
Priscilla B S Albuquerque, Carolina B Malafaia
Since the last two decades, the use of synthetic materials has increased and become more frequent in this capitalist system. Polymers used as raw materials are usually disposed very rapidly and considered serious damages when they return to the environment. Because of this behaviour, there was an increasing in the global awareness by minimizing the waste generated, in addition to the scientific community concern for technological alternatives to solve this problem. Alternatively, biodegradable polymers are attracting special interest due to their inherent properties, which are similar to the ones of the conventional plastics...
September 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28916380/roles-of-amino-acid-residues-h66-and-d326-in-the-creatine-kinase-activity-and-structural-stability
#18
Qing-Yun Wu, Fang Wei, Yuan-Yuan Zhu, Yu-Xue Tong, Jiang Cao, Ping Zhou, Zhen-Yu Li, Ling-Yu Zeng, Feng Li, Xiao-Yun Wang, Kai-Lin Xu
Creatine kinase (CK) is a key enzyme for cellular energy metabolism, catalyzing the reversible phosphoryl transfer from phosphocreatine to ADP in vertebrates. CK contains a pair of highly conserved amino acids (H66 and D326) which might play an important role in sustaining the compact structure of CK by linking its N- and C- terminal domains; however the mechanism is still unclear. In this study, spectroscopic, structural modeling and protein folding experiments suggested that D326A, H66P and H66P/D326A mutations led to disruption of the hydrogen bond between those two amino acid residues and form the partially unfolded state which made it easier to be inactivated and unfolded under environmental stresses, and more prone to form insoluble aggregates...
September 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28916379/carboxymethyl-fenugreek-galactomannan-gellan-gum-calcium-silicate-composite-beads-for-glimepiride-delivery
#19
Hriday Bera, Srilatha Mothe, Sabyasachi Maiti, Sridhar Vanga
Novel carboxymethyl fenugreek galactomannan (CFG)-gellan gum (GG)-calcium silicate (CS) composite beads were developed for controlled glimepiride (GLI) delivery. CFG having degree of carboxymethylation of 0.71 was synthesized and characterized by FTIR, DSC and XRD analyses. Subsequently, GLI-loaded hybrids were accomplished by ionotropic gelation technique employing Ca(+2)/Zn(+2)/Al(+3) ions as cross-linkers. All the formulations demonstrated excellent drug encapsulation efficiency (DEE, 48-97%) and sustained drug release behaviour (Q8h, 62-94%)...
September 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28911807/cellulose-nanofibrils-cnfs-from-ammophilaarenaria-a-natural-and-a-fast-growing-grass-plant
#20
Zayneb Jebali, Abdelkader Nabili, Hatem Majdoub, Sami Boufi
The production of cellulose nanofibrils (CNFs) with a high yield from Ammophilaarenariahas been explored and the ensuing CNFs were characterized in terms of morphology, crystallinity and rheological properties. Ammophilaarenaria (marram grass) is a perennial grass plant with high tolerance to drought, which is growing naturally in nutritionally poor sand with low organic matter. Fibers were extracted from the plant by conventional alkaline pulping and bleaching with a yield around 50% based on the dry weight of the plant...
September 11, 2017: International Journal of Biological Macromolecules
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