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International Journal of Biological Macromolecules

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https://www.readbyqxmd.com/read/27932260/equilibrium-adsorption-of-caffeic-chlorogenic-and-rosmarinic-acids-on-cationic-cross-linked-starch-with-quaternary-ammonium-groups
#1
Deimante Simanaviciute, Rima Klimaviciute, Ramune Rutkaite
In the present study, the equilibrium adsorption of caffeic acid (CA) and its derivatives, namely, chlorogenic (CGA) and rosmarinic (RA) acids on cationic cross-linked starch (CCS) with degree of substitution of quaternary ammonium groups of 0.42 have been investigated in relation to the structure and acidity of phenolic acids. The Langmuir, Freundlich and Dubinin-Radushkevich adsorption models have been used to describe the equilibrium adsorption of CA, CGA and RA from their initial solutions and solutions having the equimolar amount of NaOH at different temperatures...
December 5, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27932259/a-comparative-study-for-lipase-immobilization-onto-alginate-based-composite-electrospun-nanofibers-with-effective-and-enhanced-stability
#2
Yasemin İspirli Doğaç, İlyas Deveci, Bedrettin Mercimek, Teke Mustafa
In this study, lipase was successfully immobilized on polyvinyl alcohol/alginate and polyethylene oxide/alginate nanofibers that were prepared by electrospinning. Results showed that nanofibers (especially polyvinyl alcohol/alginate) enhanced the stability properties of lipase. When the free lipase lost its all activity after 40-60min at high temperatures, both lipase immobilized nanofibers kept almost 65-70% activity at the same time. The lipase immobilized poly vinyl alcohol/alginate and polyethylene oxide/alginate nanofibers protected approximately all of their activities until pH 9...
December 5, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27932258/heteroglycan-of-an-edible-mushroom-pleurotus-cystidiosus-structural-characterization-and-study-of-biological-activities
#3
Bibhash C Panda, Prasenjit Maity, Ashis K Nandi, Manabendra Pattanayak, Dilip K Manna, Soumitra Mondal, Satyajit Tripathy, Somenath Roy, Krishnendu Acharya, Syed S Islam
A water soluble heteroglycan (PCPS) was isolated from the aqueous extract of an edible mushroom Pleurotus cystidiosus. Structural characterization of the heteroglycan was carried out using total hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and 1D/2D NMR experiments. Sugar analysis indicated the presence of glucose, galactose, and mannose in a molar ratio of nearly 6:2:1 respectively. The chemical and NMR analysis of the PCPS indicated the presence of a repeating unit with a backbone consisting of one unit of (1→6)-b-D-glucopyranosyl, two (1→3)-b-D-glucopyranosyl, one (1→3)-a-D-glucopyranosyl, one (1→6)-a-D-glucopyranosyl, and two (1→6)-a-D-galactopyranosyl moieties respectively, out of which one (1→3)-b-D-glucopyranosyl residue was branched at O-6 with terminal b-D-glucopyranosyl and another (1→6)-a-D-galactopyranosyl residue was branched at O-2 with terminal b-D-mannopyranosyl moiety...
December 5, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27932257/antioxidant-antibacterial-and-anti-aging-activities-of-intracellular-zinc-polysaccharides-from-grifola-frondosa-sh-05
#4
Chen Zhang, Zheng Gao, Chunlong Hu, Jianjun Zhang, Xinyi Sun, Chengbo Rong, Le Jia
In present work, the strain of Grifola frondosa SH-05 was used as a vector of zinc biotransformation to produce the IZPS. The bioactivities including antioxidant and antibacterial activities in vitro and anti-aging properties in vivo of IZPS were investigated comparing with the IPS. The results which were in consistent with the results of histopathology assay demonstrated that the IZPS had superior antioxidant and anti-aging activities by scavenging the hydroxyl and DPPH radicals, increasing enzyme activities, decreasing the MDA contents and ameliorating the anile condition of mice...
December 5, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27932256/a-panorama-of-bacterial-inulinases-production-purification-characterization-and-industrial-applications
#5
REVIEW
Ram Sarup Singh, Kanika Chauhan, John F Kennedy
Inulinases are important hydrolysing enzymes which specifically act on β-2, 1 linkages of inulin to produce fructose or fructooligosaccharides. Fungi, yeasts and bacteria are the potent microbial sources of inulinases. The data on bacterial inulinases is scarce as compared to other microbial sources. Inulinases yield from bacteria is very less as compared to fungal and yeast sources of inulinases. Submerged fermentation (SmF) is the method of choice for the production of inulinases from bacterial sources. Moreover, inulin is a potent substrate for the production of inulinases in SmF...
December 5, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27923567/an-experimental-modeling-of-trinomial-bioengineering-crp-rdna-and-transporter-engineering-within-single-cell-factory-for-maximizing-two-phase-bioreduction
#6
Souvik Basak, Sumanta Kumar Ghosh, Vinay Deep Punetha, Ashish N Aphale, Prabir K Patra, Nanda Gopal Sahoo
A carbonyl reductase (cr) gene from Candida glabrata CBS138 has been heterologously expressed in cofactor regenerating E. coli host to convert Ethyl-4-chloro-3-oxobutanoate (COBE) into Ethyl-4-chloro-3-hydroxybutanoate (CHBE). The CR enzyme exhibited marked velocity at substrate concentration as high as 363mM with highest turnover number (112.77±3.95s(-1)). Solitary recombineering of such catalytic cell reproduced CHBE 161.04g/L per g of dry cell weight (DCW). Introduction of combinatorially engineered crp (crp*, F136I) into this heterologous E...
December 3, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27923566/flavonolignans-inhibit-adp-induced-blood-platelets-activation-and-aggregation-in-whole-blood
#7
Michal Bijak, Rafal Szelenberger, Joanna Saluk, Pawel Nowak
Flavonolignans are a group of active chemical compounds presented in a silymarin - a standardized extract obtained from fruits and seeds of Milk thistle (Silybum marianum L. Gaernt.). Since the 70s of the last century, flavonolignans have been regarded to the official medicine as a substances having hepatoprotective properties. However many researches performed in recent years have demonstrated that flavonolignans posses many other healthy properties including modulation of variety cell-signaling pathways. The aim of our study was to examine the effects of three major flavonolignans (silybin, silychristin and silydianin) on ADP-induced blood platelet activation using the flow cytometry analysis as well as determine the mechanism of this interaction by bioinformatic ligand docking method...
December 3, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919818/plga-soya-lecithin-based-micelles-for-enhanced-delivery-of-methotrexate-cellular-uptake-cytotoxic-and-pharmacokinetic-evidences
#8
Anupama Singh, Nagarani Thotakura, Rajendra Kumar, Bhupinder Singh, Gajanand Sharma, Om Prakash Katare, Kaisar Raza
Biocompatible and biodegradable polymers like PLGA have revolutionized the drug delivery approaches. However, poor drug loading and substantially high lipophilicity, pave a path for further tailing of this promising agent. In this regard, PLGA was feathered with biocompatible phospholipid and polymeric micelles were developed for delivery of Methotrexate (MTX) to cancer cells. The nanocarriers (114.6nm±5.5nm) enhanced the cytotoxicity of MTX by 2.13 folds on MDA-MB-231 cells. Confocal laser scanning microscopy confirmed the increased intracellular delivery...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919817/selective-adsorption-of-cr-vi-ions-from-aqueous-solutions-using-cr-6-imprinted-pebax-chitosan-go-aptes-nanofibrous-adsorbent
#9
Mohammadmahdi Etemadi, Saman Samadi, Shabnam Sharif Yazd, Pooya Jafari, Negin Yousefi, Majid Aliabadi
In the present study, a novel Cr(6+)-imprinted Pebax/chitosan/GO/APTES nanofibrous adsorbent (Cr(6+)-PCGA) was prepared and its performance was compared with PCGA nanofibers for selective sorption of Cr(VI) ions from aqueous solutions. The prepared nanofibers were characterized using FTIR, SEM and EDAX analysis. The influence of batch sorption experiments including GO/APTES content, pH, contact time, Cr(VI) initial concentration and temperature on the Cr(VI) sorption efficiency using synthesized nanofibers was investigated...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919816/surface-rearrangement-of-adsorbed-egcg-mucin-complexes-on-hydrophilic-surfaces
#10
J McColl, R Horvath, G E Yakubov, J J Ramsden
The kinetic adsorption-desorption behaviour of porcine gastric mucin in the presence of physiologically relevant concentrations of the polyphenol epigallocatechin gallate (EGCG) was investigated using high-resolution kinetic optical waveguide lightmode spectroscopy (OWLS) and atomic force microscopy (AFM). Comparison with dynamic light scattering results from EGCG-mucin mixtures indicates that discrete particles are formed whose size increases with increasing EGCG:mucin ratio. These particles are deduced to be the adsorbing entities, which fuse on the surface to form complex surface layers...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919815/chemical-modification-of-functionalized-polyhydroxyalkanoates-via-click-chemistry-a-proof-of-concept
#11
Samuel Nkrumah-Agyeefi, Carmen Scholz
A novel approach to the post-biosynthetic chemical modification of bromo and alkyne functionalized poly(3-hydroxyalkanoates), (PHAs), via copper-catalyzed azide-alkyne cycloaddition (CuAAC) and strain promoted azide alkyne cycloaddition (SPAAC) is reported. Optimum conditions for the biosynthesis of the PHA copolymers, poly(3-hydroxynonanoate-co-3-hydroxy-11-bromoundecanoate) (PHNUBr) and poly(3-hydroxynonanoate-co-3-hydroxy-10-undecynoate) (PHNUD), using Pseudomonas oleovorans as cell factories were 20h of fermentation time and a total carbon substrate concentration of 40mM...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919814/basil-seed-mucilage-as-a-new-source-for-electrospinning-production-and-physicochemical-characterization
#12
Forouzan Kurd, Milad Fathi, Hajar Shekarchizadeh
In this study nanofibers were prepared via the electrospinning technique by basil seed mucilage (BSM) as a new source of hydrocolloid. Polyvinyl alcohol (PVA) was used as an aiding agent. Viscosity and electrical conductivity of BSM/PVA solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were in the range of 790-1300cP and 745-2140μS/cm, respectively. Morphology of nanofibers were investigated using scanning electron microscopy (SEM) that showed an average diameter range of 179-390nm...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919813/designing-of-a-novel-dextransucrase-efficient-in-synthesizing-oligosaccharides
#13
Qiu-Ping Li, Chao Wang, Hong-Bin Zhang, Xue-Qin Hu, Ruo-Han Li, Jing-Han Hua
Dextransucrase (EC2.4.5.1) from strain Leuconostoc mesenteroides 0326, which synthesizes dextran and oligosaccharides, which act as prebiotics, are popularly used in such industries as food and medicine. A novel dextransucrase efficient in synthesizing oligosaccharides was designed. We constructed the truncation mutant DSR-S1-ΔA (residues 1-3087bp) by deleting the 1494bp fragment of the C-terminal. The novel enzyme (MW: 110kDa) loss activity, when sucrose was used as only substrate. After adding an acceptor, DSR-S1-ΔA was fully activated but with heavily impaired polysaccharide synthesis ability...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919812/physicochemical-properties-and-antioxidant-activities-of-polysaccharides-from-gynura-procumbens-leaves-by-fractional-precipitation
#14
Jing-En Li, Wen-Jun Wang, Guo-Dong Zheng, Lin-Yan Li
Four new polysaccharides (GPP-20, GPP-40, GPP-60 and GPP-80) were fractionated from Gynura procumbens leaves by 20%, 40%, 60% and 80% (v/v) ethanol, successively. Their physicochemical properties including the contents of neutral sugar, uronic acid and protein, as well as the monosaccharide composition were determined. In addition, the antioxidant activities of them were investigated via the reducing power assay and scavenging capacities of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and hydroxyl free radicals, respectively...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919811/biochemical-characterization-of-a-novel-l-asparaginase-from-paenibacillus-barengoltzii-being-suitable-for-acrylamide-reduction-in-potato-chips-and-mooncakes
#15
Ran Shi, Yu Liu, Qing Mu, Zhengqiang Jiang, Shaoqing Yang
A novel L-asparaginase gene (PbAsnase) from Paenibaeillus barengoltzii CAU904 was cloned and expressed in Escherichia coli. The L-asparaginase gene was 1011bp encoding 336 amino acids. Multiple sequence alignment of PbAsnase with other known L-asparaginases revealed that the enzyme showed high similarities with some Rhizobial-type L-asparaginases, sharing the highest identity of 32% with a characterized L-asparaginase from Rhizobium etli CFN 42, suggesting that it should be a novel L-asparaginase. The recombinant L-asparaginase (PbAsnase) was purified to homogeneity and biochemically characterized...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919810/immobilization-of-indigenous-holocellulase-on-iron-oxide-fe2o3-nanoparticles-enhanced-hydrolysis-of-alkali-pretreated-paddy-straw
#16
Ajay Kumar, Surender Singh, Rameshwar Tiwari, Renu Goel, Lata Nain
The holocellulase from Aspergillus niger SH3 was characterized and found to contain 125 proteins including cellulases (26), hemicellulases (21), chitinases (10), esterases (6), amylases (4) and hypothetical protein (32). The crude enzyme was immobilized on five different nanoparticles (NPs) via physical adsorption and covalent coupling methods. The enzyme-nanoparticle complexes (ENC) were screened for protein binding, enzymatic activities and immobilization efficiency. Magnetic enzyme-nanoparticle complexes (MENC) formulation showed higher immobilization efficiency (60- 80%) for most of the enzymes...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919809/effect-of-novel-bioactive-edible-coatings-based-on-jujube-gum-and-nettle-oil-loaded-nanoemulsions-on-the-shelf-life-of-beluga-sturgeon-fillets
#17
Seyed Mohammad Taghi Gharibzahedi, Sara Mohammadnabi
Effect of jujube gum (JG; 4, 8 and 12% wt)-based nanoemulsions (NEs) containing nettle essential oil (NEO; 2, 3.5 and 5% wt) as new edible coatings was investigated to preserve Beluga sturgeon fillets (BSFs) during 15 day-refrigerated storage at 4°C. Physical (weight loss, cooking loss, color and texture), chemical (pH, FFA, PV, TBARS and TVB-N), microbiological (total and psychrotrophic bacterial counts), and sensorial characteristics of BSFs were kinetically analyzed. Preliminary studies showed that the NEs formulated with NEO lower than 5% at all JG concentrations were able to form stable coating solutions owing to the highest short-term stability (>90%) and entrapment efficiency (94...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919808/attenuation-of-amyloid-fibrillation-in-presence-of-warfarin-a-biophysical-investigation
#18
Mohammad Khursheed Siddiqi, Parvez Alam, Sumit Kumar Chaturvedi, Saima Nusrat, Yasser E Shahein, Rizwan Hasan Khan
Protein misfolding and aggregation are associated with more than twenty diseases, such as neurodegenerative diseases. The amyloid oligomers and fibrils may induce cell membrane disruption and lead to cell apoptosis. A great number of studies have focused on discovery of amyloid inhibitors which may prevent or treat amyloidosis. In this study, we used human serum albumin (HSA) as an amyloid model to test the anti-amyloid effects of warfarin (WFN), a very well-known drug for treatment of thrombosis and also used by biophysicists to characterize the specific binding site on HSA (site I of subdomain IIA)...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27916570/furan-modified-natural-rubber-a-substrate-for-its-reversible-crosslinking-and-for-clicking-it-onto-nanocellulose
#19
Eliane Trovatti, Ana Gisela Cunha, Antonio J F Carvalho, Alessandro Gandini
The conventional vulcanization process applied to elastomers is irreversible and hinders therefore their useful recycling. We demonstrate here that natural rubber can be reversibly crosslinked via the Diels-Alder coupling of furan and maleimide moieties. The furan-modified natural rubber used in this strategy was also exploited to bind it to maleimide-modified nanocellulose, thus generating a covalently crosslinked composite of these two renewable polymers.
December 1, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27916569/insight-into-the-biochemical-kinetic-and-spectroscopic-characterization-of-garlic-allium-sativum-phytocystatin-implication-for-cardiovascular-disease
#20
Mohd Faizan Siddiqui, Azaj Ahmed, Bilqees Bano
Phytocystatins are cysteine proteinase inhibitors present in plants. They play crucial role in maintaining protease-anti protease balance and are involved in various endogenous processes. Thus, they are suitable and convenient targets for genetic engineering which makes their isolation and characterisation from different sources the need of the hour. In the present study a phytocystatin has been isolated from garlic (Allium sativum) by a simple two-step process using ammonium sulphate fractionation and gel filtration chromatography on Sephacryl S-100HR with a fold purification of 152...
December 1, 2016: International Journal of Biological Macromolecules
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