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International Journal of Biological Macromolecules

Nermeen M Abu-Elala, Huda O AbuBakr, Marwa S Khattab, Samah H Mohamed, Maha A El-Hady, Rehab A Ghandour, Rania E Morsi
Despite the potential antimicrobial and water purification benefits of chitosan-based nanocomposites, there are growing concerns regarding the hazards of leached nanoparticles (NPs) to the in-contact circumference. The antibacterial performance of the nanocomposites of chitosan with silver and copper NPs and carbon nanotubes was assessed with an emphasis on their impact on fish health. The minimal inhibitory concentrations of each preparation and the growth curves of Aeromonas hydrophila exposed to different nanocomposites were measured...
March 12, 2018: International Journal of Biological Macromolecules
Sukhendu Mandal, Semanti Ghosh, Debabrata Sinha, Soham Seal, Avisek Mahapa, Soumitra Polley, Deeya Saha, Keya Sau, Angshuman Bagchi, Subrata Sau
SarA, a winged-helix DNA binding protein, is a global virulence regulator in Staphylococcus aureus. The putative DNA binding region of SarA is located between amino acid residues Leu 53 and Gln 97. Previous studies have demonstrated that residues at positions 84, 88, 89, and 90 are critical for its function. To precisely understand the roles of the DNA binding residues, we have investigated nine mutants of a recombinant SarA (rSarA) along with the rSarA mutants carrying mutations at the above four positions...
March 12, 2018: International Journal of Biological Macromolecules
Abdelmageed M Othman, Maysa A Elsayed, Ali M Elshafei, Mohamed M Hassan
Agaricus bisporus CU13 laccase was purified using ammonium sulfate precipitation (40-80%), Sephadex G100, and DEAE Sephadex A50 anion exchange column chromatography, respectively. Two laccase isoenzymes (Lacc1 & Lacc2) with purification folds of 1.40 and 5.81 respectively, were obtained from DEAE Sephadex A50 column. Optimal temperature and pH were recorded at 55 °C and pH 5.0 for both laccase isoenzymes using ABTS as substrate. Lacc1 was more thermostable than Lacc2 with residual activity of 95, 80 and 6%, while Lacc2 only retained 72, 25 and 0...
March 12, 2018: International Journal of Biological Macromolecules
Jasmin L Whittaker, Rajkamal Balu, Robert Knott, Liliana de Campo, Jitendra P Mata, Christine Rehm, Anita J Hill, Naba K Dutta, Namita Roy Choudhury
Regenerated Bombyx mori silk fibroin (RSF) is a widely recognized protein for biomedical applications; however, their hierarchical gel structures are poorly understood. In this paper, the hierarchical structure of photocrosslinked RSF and RSF-based hybrid hydrogel systems: (i) RSF/Rec1-resilin and (ii) RSF/poly(N-vinylcaprolactam (PVCL) is reported for the first time using small-angle scattering (SAS) techniques. The structure of RSF in dilute to concentrated solution to fabricated hydrogels were characterized using small angle X-ray scattering (SAXS), small angle neutron scattering (SANS) and ultra-small angle neutron scattering (USANS) techniques...
March 12, 2018: International Journal of Biological Macromolecules
Audrius Gegeckas, Aistė Šimkutė, Renata Gudiukaitė, Donaldas Jonas Čitavičius
Solid keratin-rich waste management is one of essential research area in nowadays. Conventional chemical and high thermal keratin waste decomposition methods are fully explored and not enough effective for future biotechnology perspectives. However, traditional keratin-rich waste decomposition methods could be replaced by environmentally-friendly and economical microbial keratin waste biodegradation methods without energy wastage and essential amino acids and nutrition elements loss. In this study BPKer and BAKer keratinolytic peptidases from Bacillus sp...
March 12, 2018: International Journal of Biological Macromolecules
Qian Zhou, Li Cui, Lamei Ren, Ping Wang, Chao Deng, Qiang Wang, Xuedong Fan
Chitooligosaccharide (COS) has the characteristic of antioxidant and antibacterial effects. To improve the properties of silk fibroin (SF), COS was enzymatically grafted onto SF membranes using a laccase from Trametes versicolor. d-Glucosamine hydrochloride (GAH) and p-hydroxyphenylacetamide (PHAD), as the model compound of COS and tyrosine residues in SF were utilized to disclose the grafting mechanism, respectively. The data from UPLC-TQD and GPC analysis implied that laccase might catalyze the oxidation of PHAD and led to the formation of self-polymerized products...
March 11, 2018: International Journal of Biological Macromolecules
Mingsheng Yang, Lu Song, Jianhang Mao, Yuxia Shi, Changjing Wu, Yunxia Zhang, Li Huang, Weifeng Peng, Xiaomeng Liu
Complete mitochondrial genome (mitogenome) of the Omiodes indicata was sequenced and characterized. The circular mitogenome is 15,367 bp long, including 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an A + T-rich region. Nucleotide composition is highly biased toward A + T nucleotides (81.6%). All 13 PCGs initiate with canonical start codon (ATN), except for cox1 that initiates with CGA. All tRNAs have a typical clover-leaf structure, except for trnS1 (AGN) in which the base pairs of the dihydrouridine (DHU) arm are reduced...
March 11, 2018: International Journal of Biological Macromolecules
Gourhari Chakraborty, Ravi Babu Valapa, G Pugazhenthi, Vimal Katiyar
In order to overcome the challenges that underlies in dispersion of exfoliated graphene (GR) into poly lactic acid (PLA) matrix by extrusion, a versatile approach is used in this work, where uniform coating of GR over PLA is carried out prior to extrusion of PLA. Effect of melt processing on different graphene loaded composites (PLA-M-0.05GR, PLA-M-0.2GR) is studied. Investigation on the morphology, thermal stability, crystallization property, surface wettability, mechanical property, and dynamic mechanical property of composites are performed...
March 10, 2018: International Journal of Biological Macromolecules
G Zhou, K P Wang, H W Liu, L Wang, X F Xiao, D D Dou, Y B Fan
Owing to low bearing capacity and efficiency, traditional filters or adsorbents for removal of contaminants like crystal violet (CV) dye required frequent replacement. Besides, the combination of three-dimensional (3D) printing and bionics could break the constraints of traditional configuration. In this study, a novel depth-type hybrid polylactic acid (PLA)@graphene oxide (GO)/chitosan (CS) sponge filter with bionic fish-mouth structure was prepared and fabricated, assisted by 3D printing and double freeze-drying technology, according to the theories of vertical cross-step filtration and swirling flow...
March 9, 2018: International Journal of Biological Macromolecules
Zhenhui Wang, Zhimeng Lv, Chenghua Li, Yina Shao, Weiwei Zhang, Xuelin Zhao
We have previously confirmed that β-integrin from Apostichopus japonicus (designated AjITGB) binds LPS and mediates the immune response. In this study, we found that AjITGB positively promoted the echinoderm immune cells located in the coelomic cavity (designated as coelomocyte) phagocytic activities. Flow cytometry assay indicated that the phagocytic rate was significantly decreased, by a 37.3% and 41.36%, after AjITGB siRNA inference in vitro and in vivo, respectively, a result consistent with the decrease observed with an anti-AjITGB antibody blocking treatment...
March 8, 2018: International Journal of Biological Macromolecules
Joanna Filipowska, Joanna Lewandowska-Łańcucka, Adriana Gilarska, Łukasz Niedźwiedzki, Maria Nowakowska
The aim of this study was to assess osteogenic potential of three groups of biopolymeric hydrogel-based surfaces made of plain collagen, chitosan or collagen/chitosan, crosslinked with genipin or all three biopolymers modified with silica particles of two sizes (S1=240nm and S2=450nm). Biocompatibility and osteoinductive properties of the resulting composites were analyzed in the human bone marrow-derived mesenchymal stromal cells (hBMSCs) in vitro cultures. It was revealed that all tested materials are biocompatible and significantly enhance ALP activity in hBMSCs which was particularly pronounced for collagen/chitosan based hybrids...
March 8, 2018: International Journal of Biological Macromolecules
Wan Hasnidah Wan Osman, Meng-I Lin, Keiko Kondo, Takashi Nagata, Masato Katahira
Glutathione S-transferases (GSTs) of wood-degrading fungi play essential roles in cellular detoxification processes and endogenous metabolism. Fungal GSTs of GSTFuA class are suggested to be involved in lignin degradation. Ceriporiopsis subvermispora is one of the important model fungi of the selective lignin degraders, we found it interesting to study its GSTs. Here, we characterized the activities of two GSTs of the GSTFuA class of C. subvermispora (CsGST63524 and CsGST83044). A high-yield expression systems involving Escherichia coli was developed for each of these enzymes...
March 7, 2018: International Journal of Biological Macromolecules
Sangily Jayanthi, Baskaralingam Vaseeharan, Ramachandran Ishwarya, Sivashanmugam Karthikeyan, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Csaba Vágvölgyi
Prophenoloxidase is a conserved Cu-containing enzyme acting as a major defense molecule in the immune response of crustaceans. In the present research, we purified prophenoloxidase from the haemolymph of Portunus pelagicus (Pp-proPO) by Blue Sepharose CL-6B chromatography. Pp-proPO exhibited only one band with molecular weight of 75 kDa on SDS-PAGE. The purified Pp-proPO was characterized through X-ray diffraction (XRD) and high-performance liquid chromatography (HPLC). Pp-proPO showed phagocytic activity on the yeast Saccharomyces cerevisiae as well as encapsulation on sepharose CL-6B beads associated with CM sepharose and beads of sodium alginate...
March 7, 2018: International Journal of Biological Macromolecules
Abbas Shali, Sadegh Hasannia, Fatemeh Gashtasbi, S Masoud Abdous, S Shirin Shahangian, Shirin Jalili
Since anthrax is an acute infectious disease, detection and neutralization of the toxins of pathogenic Bacillus anthracis are of great importance. The critical role of protective antigen (PA) component of tripartite anthrax toxin in toxin entry into the host cell cytosol provided a great deal of effort to generate monoclonal antibodies against this constitute. Regarding the importance of anthrax detection/neutralization and unique physicochemical and pharmacological features of VHHs as single domain antibodies, the present study aimed to generate VHHs against the receptor binding domain of PA, termed PAD4...
March 7, 2018: International Journal of Biological Macromolecules
Alyne Marem, Debora N Okamoto, Lilian C G Oliveira, Diego M Ruiz, Roberto A Paggi, Marcia Y Kondo, Iuri E Gouvea, Maria A Juliano, Rosana E de Castro, Luiz Juliano, Marcelo Y Icimoto
Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon Natrialba magadii. Many extracellular proteases have been characterized from archaea to bacteria as adapted to hypersaline environments retaining function and stability until 4.0 M NaCl. As observed in other secreted halolysins, this stability can be related to the presence of a C-terminal extension (CTE) sequence. In the present work, we compared the biochemical properties of recombinant Nep protease with the truncated form at the 134 amino acids CTE (Nep∆CTE), that was more active in 4 M NaCl than the non-truncated wild type enzyme...
March 7, 2018: International Journal of Biological Macromolecules
Lin Zhou, Lu Wang, Shuju Bai, Shu Xing, Wannan Li, Junfeng Ma, Xueqi Fu
Low molecular weight protein tyrosine phosphatase (LMW-PTP) is highly conserved across almost all living organisms and is involved in the modulation of a number of cellular proteins related to important signaling pathways. In this study, we isolated lmwptp (Y94H6A.7) of Caenorhabditis elegans, the homolog of human ACP1, and set up an effective feeding-based RNA interference (RNAi) knockdown against this gene. We found that knockdown of lmwptp decreased damage associated with heat shock, oxidative stress and UV irradiation in wild-type worms, however, its deficiency didn't further reduce the stress resistance of hsf-1 or daf-16 mutants and didn't further increase the stress sensitivity associated with age-1, akt-1 or akt-2 mutants, but it enhanced the stress resistance of daf-2 mutants...
March 7, 2018: International Journal of Biological Macromolecules
Yujuan Li, Shuwen Guan, Cong Liu, Xinhua Chen, Yuemei Zhu, Yutong Xie, Jianbin Wang, Xue Ji, Liqin Li, Zhuohan Li, Yue Zhang, Xiangzhi Zeng, Mingquan Li
This study aims to investigate the neuroprotective effects of Coptis chinensis Franch polysaccharide (CCP) on Aβ1-42 transgenic CL4176 Caenorhabditis elegans, as well as its mechanism of action. The results in life span experiment showed that CCP could significantly increase the lifespan of C. elegans and the effect is in the descending order of 100 mg/L > 500 mg/L > 200 mg/L. The behavioral experiments also demonstrated that CCP at the concentration of 100 mg/L could delay the paralysis rate of C...
March 7, 2018: International Journal of Biological Macromolecules
Jianhao Wang, Zhilan Zhu, Xiang Wang, Li Yang, Li Liu, Jianpeng Wang, Eseosaserea Igbinigie, Xiaoqian Liu, Jinping Li, Lin Qiu, Yong-Qiang Li, Pengju Jiang
Recently, the in-capillary electrophoresis assay has been applied to variety kinds of analyses owing to its multiple functional integrating features, including mixing of samples, reaction process of the mixtures, and the separation and detection in one capillary system. However, the micro-reactor still has its limitations to the currently available applications, especially the mixing step of the samples inside the capillary could not be well controlled automatically or manually. Herein, we have developed a novel capillary electrophoresis assay for the detection of antibody-peptide binding inside a bending capillary...
March 7, 2018: International Journal of Biological Macromolecules
Meirong Bai, Jing He, Liangfa Kang, Jun Nie, Ruixue Yin
Individual insulin therapy considering the heterogeneity of insulin resistance between patients may bring more benefits than conventional therapy. Therefore, in glucose-responsive insulin delivery systems, more attention should be paid on further regulation of insulin release to meet individual requirements. Our study shows the feasibility of using a photo-crosslinkable shell layer to regulate basal and bolus insulin release from glucose-responsive Con A-polysaccharides network. Core-shell microspheres were fabricated through a two-step high-speed shear-emulsification method...
March 7, 2018: International Journal of Biological Macromolecules
Shang-Jun Yin, Linmeng Zhang, Lili Zhang, Jiaxin Wan, Wei Song, Xiamin Jiang, Yong-Doo Park, Yue-Xiu Si
The pharaoh cuttlefish Sepia pharaonis is particularly sensitive to environmental changes in its breeding environment. The breeding of S. pharaonis larvae was carried out in different salinities for 48 h, and the changes in survival rate, histological structure, energy metabolism, and anti-oxidative stress parameters were investigated and correlated with arginine kinase (AK) expression changes in muscle and liver tissues. The suitable salinity for larvae cultivation ranged from 24 to 30‰, and the survival rate showed a significant decline at 21‰ salinity...
March 7, 2018: International Journal of Biological Macromolecules
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