journal
MENU ▼
Read by QxMD icon Read
search

European Journal of Cell Biology

journal
https://www.readbyqxmd.com/read/28889942/differential-functions-of-wave-regulatory-complex-subunits-in-the-regulation-of-actin-driven-processes
#1
Christof Litschko, Joern Linkner, Stefan Brühmann, Theresia E B Stradal, Tobias Reinl, Lothar Jänsch, Klemens Rottner, Jan Faix
The WAVE regulatory complex (WRC) links upstream Rho-family GTPase signaling to the activation of the ARP2/3 complex in different organisms. WRC-induced and ARP2/3 complex-mediated actin nucleation beneath the plasma membrane is critical for actin assembly in the leading edge to drive efficient cell migration. The WRC is a stable heteropentamer composed of SCAR/WAVE, Abi, Nap, Pir and the small polypeptide Brk1/Hspc300. Functional interference with individual subunits of the complex frequently results in diminished amounts of the remaining polypeptides of the WRC complex, implying the complex to act as molecular entity...
September 4, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28886883/alternative-splicing-of-telomerase-catalytic-subunit-htert-generated-by-apoptotic-endonuclease-endog-induces-human-cd4-t-cell-death
#2
Dmitry D Zhdanov, Daria A Vasina, Vladimir A Grachev, Elena V Orlova, Valentina S Orlova, Marina V Pokrovskaya, Svetlana S Alexandrova, Nikolai N Sokolov
Telomerase activity is regulated by alternative splicing of its catalytic subunit human Telomerase Reverse Transcriptase (hTERT) mRNA. Induction of a non-active spliced hTERT leads to inhibition of telomerase activity. However, very little is known about the mechanism of hTERT mRNA alternative splicing. The aim of this study was to determine the role of the apoptotic endonuclease EndoG in alternative splicing of hTERT and telomerase activity. A strong correlation was identified between EndoG expression levels and hTERT splice variants in human CD4(+) and CD8(+) T lymphocytes...
September 1, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28865806/resveratrol-promotes-human-embryonic-stem-cells-self-renewal-by-targeting-sirt1-erk-signaling-pathway
#3
Zahra Safaeinejad, Mohammad Nabiuni, Maryam Peymani, Kamran Ghaedi, Mohammad Hossein Nasr-Esfahani, Hossein Baharvand
Resveratrol (RSV), a natural polyphenol component, has diverse biological properties. It has been shown that RSV regulated the self-renewal and differentiation of several types of stem cells, but the precise role of this compound on regulation of human embryonic stem cells (hESCs) self- renewal remained to be elucidated. Here we have shown that RSV promoted hESCs proliferation through cell cycle modulation and up-regulation of anti-apoptotic markers, without affecting pluripotency. Furthermore, inhibition of SIRT1 by EX-527 resulted in suppression of RSV-induced enhancement of hESCs self-renewal...
August 26, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28847588/cellular-distribution-of-invadopodia-is-regulated-by-nanometer-scale-surface-protein-patterns
#4
Gizem Bati-Ayaz, Ali Can, Devrim Pesen-Okvur
Invadopodia are proteolytic structures formed by cancer cells. It is not known whether their cellular distribution can be regulated by the organization of the extracellular matrix or the organization of the golgi complex or whether they have an adhesion requirement. Here, we used electron beam lithography to fabricate fibronectin (FN) nanodots with isotropic and gradient micrometer scale spacings on K-casein and laminin backgrounds. Investigating cancer cells cultured on protein nanopatterns, we showed that (i) presence of FN nanodots on a K-casein background decreased percent of cells with neutral invadopodia polarization compared to FN control surfaces; (ii) presence of a gradient of FN nanodots on a K-casein background increased percent of cells with negative invadopodia polarization compared to FN control surfaces; (iii) polarization of the golgi complex was similar to that of invadopodia in agreement with a spatial link; (iv) local adhesion did not necessarily appear to be a prerequisite for invadopodia formation...
August 23, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28818340/lps-binding-to-hmgb1-promotes-angiogenic-behavior-of-endothelial-cells-through-inhibition-of-p120-and-cd31-via-erk-p38-src-signaling
#5
Zhenguo Liu, Juan Wang, Wu Xing, Yingqiong Peng, Jun Quan, Xuegong Fan
Lipopolysaccharide (LPS) is known to mediate angiogenic effects in endothelial cells. The underlying mechanisms, however, remain largely unknown. In this study, we showed that LPS induced high motility group box protein 1 (HMGB1) secretion in human pulmonary microvascular endothelial cells (HPMECs). Knockdown and overexpression of HMGB1 by adenoviral vectors effectively inhibited and promoted LPS-induced HPMEC migration and capillary-like tube formation, respectively. On the other hand, HMGB1 exerted an inhibitory effect on LPS-suppressed expression of platelet-endothelial cell adhesion molecule (CD31) and p120 catenin (p120); HMGB1 knockdown reversed this effect...
August 9, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28797528/actinin-1-and-actinin-4-play-essential-but-distinct-roles-in-invadopodia-formation-by-carcinoma-cells
#6
Hideki Yamaguchi, Yuumi Ito, Nami Miura, Yuko Nagamura, Ayaka Nakabo, Kiyoko Fukami, Kazufumi Honda, Ryuichi Sakai
Invadopodia are ventral membrane protrusions formed by cancer cells that degrade the extracellular matrix (ECM) during tumor invasion and metastasis. Formation of invadopodia is initiated by the assembly of actin filaments (F-actin) that results from the coordinated activation of several actin regulatory proteins. Actinin-1 and actinin-4 are actin bundling proteins expressed in non-muscle cells and actinin-4 is preferentially associated with malignant phenotypes of carcinoma cells. In this study, we investigated the role of actinin-1 and -4 in invadopodia formation...
August 1, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28780966/elastic-tethers-connect-separating-anaphase-chromosomes-in-a-broad-range-of-animal-cells
#7
Arthur Forer, Michelle L Duquette, Leocadia V Paliulis, E Fegaras, M Ono, D Preece, Michael W Berns
We describe the general occurrence in animal cells of elastic components ("tethers") that connect individual chromosomes moving to opposite poles during anaphase. Tethers, originally described in crane-fly spermatocytes, exert force on chromosome arms opposite to the direction the anaphase chromosomes move. We show that they exist in a broad range of animal cells. Thus tethers are previously unrecognised components of general mitotic mechanisms that exert force on chromosomes and they need to be accounted for in general models of mitosis in terms of forces on chromosomes and in terms of what their roles might be...
July 29, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28764863/cellular-expression-in-vitro-and-in-vivo-confirmation-of-gaec1-oncogenic-properties-in-colon-cancer
#8
Riajul Wahab, Vinod Gopalan, Farhadul Islam, Robert A Smith, Bin Qiao, Alfred K Lam
GAEC1 (Gene amplified in esophageal cancer 1) alterations have oncogenic properties in oesophageal squamous cell carcinomas and frequent amplifications of the gene were noted in colorectal adenocarcinomas. However, the subcellular localization and expression of GAEC1 at the protein level have never been reported in human cancer cells. The present study aimed to investigate whether GAEC1 is differentially expressed in different stages of colon cancer and to elucidate its underlying cellular and molecular mechanism in colon cancer progression...
July 26, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28764862/ferulic-acid-promotes-osteogenesis-of-bone-marrow-derived-mesenchymal-stem-cells-by-inhibiting-microrna-340-to-induce-%C3%AE-catenin-expression-through-hypoxia
#9
Kewei Du, Ziqiang Li, Xuchen Fang, Tingwei Cao, Yaozeng Xu
Osteogenic differentiation is regulated through multiple signaling networks that may include responses to hypoxia. Antioxidant ferulic acid (FA) can promote hypoxia signaling by inducing hypoxic-induced factor (HIF). However, whether FA could affect osteogenesis has not been explored. We examined human bone marrow-derived mesenchymal stem cell (MSC) following FA treatment. The expression of β-catenin was measured, and candidate microRNAs that target β-catenin were studied. The involvement of hypoxia was investigated in miR-340-5p that contains hypoxia response elements (HRE) in the promoter region...
July 22, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28754257/ejcb-molecular-basis-of-protein-fates-in-the-secretory-and-endocytic-pathways-and-beyond
#10
EDITORIAL
Frédéric Couture, Anna Mai Jansen, Paul Taghert, Klaudia Brix
No abstract text is available yet for this article.
August 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28684042/signal-dependent-transport-of-a-membrane-cargo-from-early-endosomes-to-recycling-endosomes
#11
Ismail S Mahmoud, Jade Louber, Steve K Dower, Anne M Verhagen, Paul A Gleeson
Many membrane cargoes undergo endocytosis and intracellular recycling to the plasma membrane via the early endosomes and the recycling endosomes. However whether specific sorting signals are required for transport from early endosomes to recycling endosomes is not known and the current view is that transport to the recycling endosomes is by a passive default process. Here we show that the cytoplasmic tail of the neonatal Fc receptor (FcRn) contains discrete signals for endocytosis and for sorting to the recycling endosomes...
August 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28624236/regulation-of-the-proprotein-convertases-expression-and-activity-during-regenerative-angiogenesis-role-of-hypoxia-inducible-factor-hif
#12
Jia Ma, Serge Evrard, Iker Badiola, Geraldine Siegfried, Abdel-Majid Khatib
Proprotein convertases (PCs) are involved in various physiological and pathological processes ranging from embryogenesis to carcinogenesis. Here, using the zebrafish fin regeneration model, we report induced expression of furin and PC5 but not PACE4 and PC7 during fin regeneration that is associated with increased PC activity. Stabilization of HIF by cobalt chloride (CoCl2) further increases these processes. The use of the general PC-inhibitor decanoyl-RVKR-cholromethyl ketone (CMK) inhibited control and CoCl2-induced PC activity...
August 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28483279/macrocyclization-of-a-potent-pace4-inhibitor-benefits-and-limitations
#13
Teresa Łepek, Anna Kwiatkowska, Frédéric Couture, Kévin Ly, Roxane Desjardins, Yves Dory, Adam Prahl, Robert Day
PACE4, one of the seven members of the proprotein convertase family, plays an important role in the progression of prostate cancer. Therefore, its inhibition has become an attractive target to develop new therapies against this disease. Recently, we have developed a highly potent and selective PACE4 inhibitor, known as the multi-Leu peptide with the following sequence Ac-LLLLRVKR-NH2. Herein, with the aim of improving the stability profile of this inhibitor for potential in vivo application, we investigated the impact of different cyclization strategies...
August 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28413120/endosome-to-trans-golgi-network-transport-of-proprotein-convertase-7-is-mediated-by-a-cluster-of-basic-amino-acids-and-palmitoylated-cysteines
#14
Jeroen Declercq, Bruno Ramos-Molina, Ragna Sannerud, Bas Brouwers, Vincent P E G Pruniau, Sandra Meulemans, Evelyn Plets, Wim Annaert, John W M Creemers
Proprotein Convertase 7 (PC7) is a Furin-like endoprotease that cleaves precursor proteins at basic amino acids. PC7 is concentrated in the trans-Golgi network (TGN) but it shuttles between the plasma membrane and the TGN depending on sequences in the cytoplasmic tail. A short region containing a three amino acids motif, P(724)-L(725)-C(726), is essential and sufficient for internalization of PC7 but not for TGN localization, which requires the additional presence of the juxtamembrane region. In this study we have investigated the contribution of a cluster of basic amino acids and two reversibly palmitoylated cysteine residues to endocytic trafficking...
August 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28377049/the-endocytic-pathways-of-a-secretory-granule-membrane-protein-in-hek293-cells-pam-and-egf-traverse-a-dynamic-multivesicular-body-network-together
#15
Nils Bäck, Kristiina Kanerva, Vishwanatha Kurutihalli, Andrew Yanik, Elina Ikonen, Richard E Mains, Betty A Eipper
Peptidylglycine α-amidating monooxygenase (PAM) is highly expressed in neurons and endocrine cells, where it catalyzes one of the final steps in the biosynthesis of bioactive peptides. PAM is also expressed in unicellular organisms such as Chlamydomonas reinhardtii, which do not store peptides in secretory granules. As for other granule membrane proteins, PAM is retrieved from the cell surface and returned to the trans-Golgi network. This pathway involves regulated entry of PAM into multivesicular body intralumenal vesicles (ILVs)...
August 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28372832/cab45-unraveling-key-features-of-a-novel-secretory-cargo-sorter-at-the-trans-golgi-network
#16
REVIEW
Birgit Blank, Julia von Blume
The accurate and efficient delivery of proteins to specific domains of the plasma membrane or to the extracellular space is critical for the ordered function of surface receptors and proteins such as insulin, collagens, antibodies, extracellular proteases. The trans-Golgi network is responsible for sorting proteins onto specific carriers for transport to their final destination. The role of the mannose-6-phosphate receptor in the sorting of hydrolases destined for lysosomes has been studied extensively, but the sorting mechanisms for secreted proteins remains poorly understood...
August 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28366434/latest-emerging-functions-of-spp-sppl-intramembrane-proteases
#17
REVIEW
Torben Mentrup, Regina Fluhrer, Bernd Schröder
Signal peptide peptidase (SPP) and the four related SPP-like (SPPL) proteases are homologues of the presenilins, which comprise the catalytic centre of the γ-secretase complex. SPP/SPPL proteases are GxGD-type aspartyl intramembrane proteases selective for substrates with a type II membrane topology. Subcellular localisations of SPP/SPPL proteases range from the early secretory pathway to the plasma membrane and the endocytic system. Similarly diverse are their functional roles at the cellular level covering the turnover of signal peptides and membrane proteins, a contribution to the ERAD pathway as well as the regulation of cellular protein glycosylation and certain signaling pathways...
August 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28347547/pace4-is-an-important-driver-of-zr-75-1-estrogen-receptor-positive-breast-cancer-proliferation-and-tumor-progression
#18
François Panet, Frédéric Couture, Anna Kwiatkowska, Roxane Desjardins, Brigitte Guérin, Robert Day
Breast cancer is the most frequent and deadly malignancy in women worldwide. Despite national screening programs combined with new treatments relapse rate remain high and new therapies are needed. From previous work, we identified PACE4, a member of the proprotein convertase (PCs) family of endoproteases, as a novel therapeutic target in prostate cancer. In the present study we asked the question if PACE4 could also be a potential target in breast cancer. In clinical samples of breast adenocarcinoma, we observed a specific overexpression of PACE4 in the estrogen-receptor (ER) positive subtype...
August 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28274595/interdependence-of-thyroglobulin-processing-and-thyroid-hormone-export-in-the-mouse-thyroid-gland
#19
Jonas Weber, Joseph McInnes, Cise Kizilirmak, Maren Rehders, Maria Qatato, Eva K Wirth, Ulrich Schweizer, Francois Verrey, Heike Heuer, Klaudia Brix
Thyroid hormone (TH) target cells need to adopt mechanisms to maintain sufficient levels of TH to ensure regular functions. This includes thyroid epithelial cells, which generate TH in addition to being TH-responsive. However, the cellular and molecular pathways underlying thyroid auto-regulation are insufficiently understood. In order to investigate whether thyroglobulin processing and TH export are sensed by thyrocytes, we inactivated thyroglobulin-processing cathepsins and TH-exporting monocarboxylate transporters (Mct) in the mouse...
August 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28129919/loss-of-huntingtin-stimulates-capture-of-retrograde-dense-core-vesicles-to-increase-synaptic-neuropeptide-stores
#20
Dinara Bulgari, David L Deitcher, Edwin S Levitan
The Huntington's disease protein Huntingtin (Htt) regulates axonal transport of dense-core vesicles (DCVs) containing neurotrophins and neuropeptides. DCVs travel down axons to reach nerve terminals where they are either captured in synaptic boutons to support later release or reverse direction to reenter the axon as part of vesicle circulation. Currently, the impact of Htt on DCV dynamics in the terminal is unknown. Here we report that knockout of Drosophila Htt selectively reduces retrograde DCV flux at proximal boutons of motoneuron terminals...
August 2017: European Journal of Cell Biology
journal
journal
27680
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"