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Journal of Inorganic Biochemistry

Mziyanda Mbaba, Amanda N Mabhula, Natasha Boel, Adrienne L Edkins, Michelle Isaacs, Heinrich C Hoppe, Setshaba D Khanye
A focused series of novobiocin derivatives containing a ferrocene unit together with their corresponding organic novobiocin analogues have been synthesized in modest to good yields. These compounds were screened for biological activity against a chloroquine-sensitive strain of Plasmodium falciparum (3D7) and human breast cancer cell line (HCC38). With the exception of compounds 5c and 5d, the general trend observed is that incorporation of the ferrocene moiety into novobiocin scaffold resulted in compounds 6a-d/6f showing enhanced activity compared to organic analogues 5a-b and 5e-f...
April 13, 2017: Journal of Inorganic Biochemistry
Sohini Mukherjee, Manjistha Mukherjee, Sabyasachi Bandyopadhyay, Abhishek Dey
The extent of heme extraction from myoglobin (Mb) by methylethyl ketone is found to be pH dependent and show three distinct phases. Parallel investigations of the protein using resonance Raman (rR) and circular dichroism (CD) across these pH regions indicate that these phases correspond to three different protonation steps in holoMb as the pH of the solution changed. The first transition occurs between pH5-6 and is due to the protonation of one of the heme propionate groups which disrupts its H-bonding with Arg 45 in the loop...
April 9, 2017: Journal of Inorganic Biochemistry
Ying Long, Mei-Pan Yang, Bing-Qin Yang
Two rhodamine-active probes RBAI (Rhodamine B-di-Aminobenzene-phenyl Isothiocyanate) and RGAI (Rhodamine 6G-di-Aminobenzene-phenyl Isothiocyanate) were designed, synthesized and characterized. The probes were developed as fluorescent and colorimetric chemodosimeters in ethanol-water solution with a broad pH span (5-10) and high selectivity toward Hg(2+) but no significant response toward other common competitive cations. The Hg(2+)-promoted ring opening of spirolactam of the rhodamine moiety induced cyclic guanylation of the thiourea moiety, which resulted in the dual chromo- and fluorogenic observation (off-on)...
April 9, 2017: Journal of Inorganic Biochemistry
Jolanta Dlugaszewska, Wojciech Szczolko, Tomasz Koczorowski, Paulina Skupin-Mrugalska, Anna Teubert, Krystyna Konopka, Malgorzata Kucinska, Marek Murias, Nejat Düzgüneş, Jadwiga Mielcarek, Tomasz Goslinski
Photodynamic therapy involves the use of a photosensitizer that is irradiated with visible light in the presence of oxygen, resulting in the formation of reactive oxygen species. A novel phthalocyanine derivative, the quaternary iodide salt of magnesium(II) phthalocyanine with N-methyl morpholiniumethoxy substituents, was synthesized, and characterized. The techniques used included mass spectrometry (MALDI TOF), UV-vis, NMR spectroscopy, and photocytotoxicity against bacteria, fungi and cancer cells. The phthalocyanine derivative possessed typical characteristics of compounds of the phthalocyanine family but the effect of quaternization was observed on the optical properties, especially in terms of absorption efficiency...
April 8, 2017: Journal of Inorganic Biochemistry
Florian Brulfert, Samir Safi, Aurélie Jeanson, Harald Foerstendorf, Stephan Weiss, Catherine Berthomieu, Sandrine Sauge-Merle, Éric Simoni
The threat of a dirty bomb which could cause internal contamination has been of major concern for the past decades. Because of their high chemical toxicity and their presence in the nuclear fuel cycle, uranium and neptunium are two actinides of high interest. Calmodulin (CaM) which is a ubiquitous protein present in all eukaryotic cells and is involved in calcium-dependent signaling pathways has a known affinity for uranyl and neptunyl ions. The impact of the complexation of these actinides on the physiological response of the protein remains, however, largely unknown...
April 8, 2017: Journal of Inorganic Biochemistry
Hong Wang, Nianhui Zhai, Ying Chen, Haibin Xu, Kehe Huang
Calcium, as a ubiquitous second messenger, governs a large array of cellular processes and is necessary for cell survival. More recently, it was observed that the cytosolic Ca(2+) concentration ([Ca(2+)]c) elevation could induce apoptosis in primary cultured rat proximal tubular (rPT) cells exposed to cadmium (Cd), but the concrete mechanism is still unclear. This study was designed to investigate the signal pathway involved in [Ca(2+)]c elevation-mediated apoptosis. The results confirmed the elevation of [Ca(2+)]c by confocal microscopy and enhancement of the apoptosis by Hoechst 33258 staining and flow cytometer when rPT cells were exposed to Cd for 12h...
April 8, 2017: Journal of Inorganic Biochemistry
Nebojša Pantelić, Bojana B Zmejkovski, Branka Kolundžija, Marija Đorđić Crnogorac, Jelena M Vujić, Biljana Dojčinović, Srećko R Trifunović, Tatjana P Stanojković, Tibor J Sabo, Goran N Kaluđerović
Four novel gold(III) complexes of general formulae [AuCl2{(S,S)-R2eddl}]PF6 (R2eddl=O,O'-dialkyl-(S,S)-ethylenediamine-N,N'-di-2-(4-methyl)pentanoate, R=n-Pr, n-Bu, n-Pe, i-Bu; 1-4, respectively), were synthesized and characterized by elemental analysis, UV/Vis, IR, and NMR spectroscopy, as well as high resolution mass spectrometry. Density functional theory calculations pointed out that (R,R)-N,N'-configuration diastereoisomers were energetically the most favorable. Duo to high cytotoxic activity complex 3 was chosen for stability study in DMSO, no decomposition occurs within 24h, and for the reaction with ascorbic acid in which was reduced immediately...
April 4, 2017: Journal of Inorganic Biochemistry
Anderson Arndt, Cleber Wanderlei Liria, Jenicer K U Yokoyama-Yasunaka, M Terêsa Machini, Sílvia Reni Bortolin Uliana, Breno Pannia Espósito
Leishmaniasis is an infection caused by protozoa of the genus Leishmania and transmitted by sandflies. Current treatments are expensive and time-consuming, involving Sb(V)-based compounds, lipossomal amphotericin B and miltefosine. Recent studies suggest that inhibition of trypanothione reductase (TR) could be a specific target in the development of new drugs because it is essential and exclusive to trypanosomatids. This work presents the synthesis and characterization of new iminodibenzyl derivatives (dado) with ethylenediamine (ea), ethanolamine (en) and diethylenetriamine (dien) and their copper(II) complexes...
April 4, 2017: Journal of Inorganic Biochemistry
Yao Zhang, Zhenlei Zhang, Yi Gou, Ming Jiang, Hamid Khan, Zuping Zhou, Hong Liang, Feng Yang
Owing to the flexible IIA sub-domain of human serum albumin (HSA), we proposed to rationally design a metal agent with a leaving group, and then regulate a leaving group of metal agent to be displaced by His-242 for improving its delivery efficiency and selectivity. To confirm our hypothesis, we synthesized a copper(II) compound derived from 2-amino-5-chlorophenol 2-hydroxybenzaldehyde Schiff-base, containing a leaving group [pyridine, PRD], namely Cu(L)(PRD). The HSA complex structure revealed that Cu(L)(PRD) binds to the hydrophobic cavity in HSA IIA sub-domain, His242 of HSA replaces the pyridine ligand in Cu compound, coordinating with Cu(2+)...
April 4, 2017: Journal of Inorganic Biochemistry
Agnieszka Strzelecka-Kiliszek, Lukasz Bozycki, Saida Mebarek, Rene Buchet, Slawomir Pikula
Bone cells control initial steps of mineralization by producing extracellular matrix (ECM) proteins and releasing vesicles that trigger apatite nucleation. Using transmission electron microscopy with energy dispersive X-ray microanalysis (TEM-EDX) we compared the quality of minerals in vesicles produced by two distinct human cell lines: fetal osteoblastic hFOB 1.19 and osteosarcoma Saos-2. Both cell lines, subjected to osteogenic medium with ascorbic acid (AA) and β-glycerophosphate (β-GP), undergo the entire osteoblastic differentiation program from proliferation to mineralization, produce the ECM and spontaneously release vesicles...
March 29, 2017: Journal of Inorganic Biochemistry
Aleksandar Dimkovikj, Mallory J Banton, Lance A McDanel, Katelin N Arndt, Katelyn E Unvert, Elizabeth K Thorn, Ana Royo Marco, Rachel A Hellmann-Whitaker
RNA is known to interact with Mg(2+) when assuming higher-ordered tertiary configurations. Structurally, when tRNA molecules interact with Mg(2+), they consistently form a "L-shape" conformation each time they are synthesized. Therefore, if Mg(2+) can induce tertiary structure formation, then binding to alternative cations could produce alternative tertiary configurations. By utilizing circular dichroism and mobility gel-shift assays it was observed that tRNA structure can be altered when in the presence of different divalent cationic species...
March 28, 2017: Journal of Inorganic Biochemistry
Xin Li, Zihui Liu, Ying Xu, Dongfeng Wang
A novel heterobinuclear complex [CoNa(C15H10NO4F)2(CH3OH)]2 with Schiff base (C15H10NO4F: 2-amino-4-fluorobenzoic acid-3-methoxysalicylaldehyde) was synthesized and characterized by IR and (1)H NMR spectroscopy, elemental analysis and single crystal X-ray diffraction. X-ray crystallography reveals that the cobalt atom is six-coordinated by two nitrogen atoms from -CN-, two carboxylate oxygen atoms and two hydroxyl oxygen atoms in different ligands, while the sodium atom is seven-coordinated by two methoxy oxygen atoms, two hydroxyl oxygen atoms in different ligands, two oxygen atoms in the same carboxylate and one oxygen atom of solvent methanol...
March 28, 2017: Journal of Inorganic Biochemistry
Cláudia S Nóbrega, Mariana Raposo, Gonzalez Van Driessche, Bart Devreese, Sofia R Pauleta
Neisseria gonorrhoeae is an obligate human pathogen that expresses an array of molecular systems to detoxify reactive oxygen species as defense mechanisms during colonization and infection. One of these is the bacterial peroxidase that reduces H2O2 to water in its periplasm. The soluble form of this enzyme was heterologously expressed in E. coli in the holo-form binding two c-types hemes, a high-potential E heme and a low-potential P heme, with redox potentials of (+310mV) and (-190mV/-300mV), respectively in the presence of calcium ions, at pH7...
March 27, 2017: Journal of Inorganic Biochemistry
Baolong Xie, Fufeng Liu, Xiaoyan Dong, Yongjian Wang, Xiaoguang Margaret Liu, Yan Sun
Aggregation of amyloid β-proteins (Aβ) induced by Cu(2+) is a crucial element in the pathogenesis of Alzheimer's disease (AD), and cerebral acidosis is a common complication of AD. Under mildly acidic conditions, Cu(2+)-Aβ species have higher tendency to generate neurotoxic aggregates. Hence it is of significance to develop potent agents that inhibit Cu(2+)-mediated Aβ aggregation under a mildly acidic condition. Herein we synthesized acidulated human serum albumin (A-HSA) to mitigate Cu(2+)-mediated Aβ42 aggregation and cytotoxicity at pH6...
March 27, 2017: Journal of Inorganic Biochemistry
Rainer Lippert, Tatyana E Shubina, Sandra Vojnovic, Aleksandar Pavic, Jovana Veselinovic, Jasmina Nikodinovic-Runic, Nada Stankovic, Ivana Ivanović-Burmazović
In order to improve antimicrobial effects of previously studied meso-tetrakis(4-ferrocenylphenyl)porphyrin 1, we have modified its structure by replacing two trans-positioned ferrocenylphenyl moieties with methoxy methylene substituted tert-butylphenyl moieties. Newly synthesized 5(4),15(4)-bis-(ferrocenyl)-10(4),20(4)-bis-(tert-butyl)-10(2),10(6),20(2),20(6)-tetrakis-(methoxy-methylene)-5,10,15,20-tetraphenylporphyrin 4 was chemically characterized in detail (by NMR, UV/Vis, IR, MALDI-TOF and ESI MS spectrometry, cyclic voltammetry, prediction of the relative lipophilicity as well as computational methods) and its biological effects were studied in terms of its antibacterial and antifungal activity (both with and without photoactivation), cytotoxicity, hemolysis and DNA cleavage...
March 21, 2017: Journal of Inorganic Biochemistry
Geng Dong, Ulf Ryde
The DMSO reductase family is the largest and most diverse family of mononuclear molybdenum oxygen-atom-transfer proteins. Their active sites contain a Mo ion coordinated to two molybdopterin ligands, one oxo group in the oxidised state, and one additional, often protein-derived ligand. We have used density-functional theory to evaluate how the fourth ligand (serine, cysteine, selenocysteine, OH(-), O(2-), SH(-), or S(2-)) affects the geometries, reaction mechanism, reaction energies, and reduction potentials of intermediates in the DMSO reductase reaction...
March 21, 2017: Journal of Inorganic Biochemistry
Qingchun Zhang, Bo Jin, Zhaotao Shi, Xiaofang Wang, Shan Lei, Xingyan Tang, Hua Liang, Qiangqiang Liu, Mei Gong, Rufang Peng
A new tris(dopamine) derivative, containing three dopamine chelate moieties which were attached to a trimesic acid molecular scaffold, has been prepared and fully characterized by NMR, FTIR and HRMS. The solution thermodynamic stability of the chelator with Fe(III), Mg(II), Zn(II) and Fe(II) ions was investigated. Results demonstrated that the chelator exhibited effective binding ability and improved selectivity to Fe(III) ion. The chelator possessed affinity similar to that of diethylenetriaminepentaacetic acid chelator for Fe(III) ion...
March 21, 2017: Journal of Inorganic Biochemistry
Massimiliano Peana, Serenella Medici, Valeria Marina Nurchi, Joanna I Lachowicz, Guido Crisponi, Eugenio Garribba, Daniele Sanna, Maria Antonietta Zoroddu
5-Hydroxy-2-(hydroxymethyl)pyridin-4(1H)-one ligand, an iron chelator, was evaluated for its coordination ability toward Al(III), Cu(II) and Zn(II) ions by using potentiometric, NMR, EPR and UV-Vis techniques. The behavior of the ligand with the non-essential Al(III) ion has been examined, as well as its potential influence on the homeostatic equilibria of the essential Cu(II) and Zn(II) ions. Structural information on the complex formation equilibria have been obtained from 1D and 2D NMR study. The donor atoms involved in the coordination of Al(III), Cu(II) and Zn(II) ions are (O, O) the same as for Fe(III) at physiological pH value, even if from the complexation competition study the ligand appears to be more selective toward Fe(III) ions supporting that it can be used as an iron chelating agent...
March 16, 2017: Journal of Inorganic Biochemistry
Justyna Krych-Madej, Lidia Gebicka
Catalase, a heme enzyme, which catalyzes decomposition of hydrogen peroxide to water and molecular oxygen, is one of the main enzymes of the antioxidant defense system of the cell. Nitrite, used as a food preservative has long been regarded as a harmful compound due to its ability to form carcinogenic nitrosamines. Recently, much evidence has been presented that nitrite plays a protective role as a nitric oxide donor under hypoxic conditions. In this work the effect of nitrite on the catalytic reactions of catalase was studied...
February 24, 2017: Journal of Inorganic Biochemistry
Jianqiao Chen, Tingru Pan, Na Wan, Zhepeng Sun, Ziwei Zhang, Shu Li
Cadmium (Cd) decreases immune function and induces apoptosis of immune cells. Selenium (Se) can antagonize some metal element toxicity including Cd. To evaluate the cytotoxicity of Cd and the chemoprotective role of Se on bird neutrophils in vitro, we incubated chicken neutrophils cells with Cadmium chloride (CdCl2) (10(-6)M), Sodium selenite (Na2SeO3) (10(-7)M), and with a mixture of Na2SeO3 (10(-7)M) and CdCl2 (10(-6)M) for 12, 24, 36, and 48h. We found that Interleukin 1β (IL-1β), Interleukin 10 (IL-10), and interferon gamma (IFN-γ) increased and interleukin 17 (IL-17), interleukin 4 (IL-4) decreased significantly in the chicken neutrophils of the Cd treatment groups...
February 24, 2017: Journal of Inorganic Biochemistry
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