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Journal of Neuroscience Methods

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https://www.readbyqxmd.com/read/28192129/optimized-solubilization-of-trizol-precipitated-protein-permits-western-blotting-analysis-to-maximize-data-available-from-brain-tissue
#1
Ashley M Kopec, Phillip D Rivera, Michael J Lacagnina, Richa Hanamsagar, Staci D Bilbo
BACKGROUND: Techniques simultaneously assessing multiple levels of molecular processing are appealing because molecular signaling underlying complex neural phenomena occurs at complementary levels. The TRIzol method isolates RNA and DNA, but protein retrieval is difficult due to inefficient solubilization of precipitated protein pellets. NEW METHOD: We optimized a protocol for the efficient solubilization of protein from TRIzol-precipitated brain tissue for Western blotting analysis, which was also more effective at directly homogenizing brain tissue than RIPA buffer...
February 10, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28192130/ielvis-an-open-source-matlab-toolbox-for-localizing-and-visualizing-human-intracranial-electrode-data
#2
David M Groppe, Stephan Bickel, Andrew R Dykstra, Xiuyuan Wang, Pierre Mégevand, Manuel R Mercier, Fred A Lado, Ashesh D Mehta, Christopher J Honey
BACKGROUND: Intracranial electrical recordings (iEEG) and brain stimulation (iEBS) are invaluable human neuroscience methodologies. However, the value of such data is often unrealized as many laboratories lack tools for localizing electrodes relative to anatomy. To remedy this, we have developed a MATLAB toolbox for intracranial electrode localization and visualization, iELVis. NEW METHOD: iELVis uses existing tools (BioImage Suite, FSL, and FreeSurfer) for preimplant magnetic resonance imaging (MRI) segmentation, neuroimaging coregistration, and manual identification of electrodes in postimplant neuroimaging...
February 9, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28185890/-recording-the-human-brainstem-frequency-following-response-in-the-free-field
#3
Nuno Gama, Isabelle Peretz, Alexandre Lehmann
BACKGROUND: The human auditory brainstem frequency-following response (FFR) is an objective measure used to investigate the brainstem's encoding ability of sounds. Traditionally, FFRs are recorded under close-field conditions (earphones), but free-field stimulations (loudspeaker) have yet to be attempted, which would increase the applications of FFRs by making this technique accessible to those who cannot wear inserted transducers. Here we test the feasibility and reliability of measuring speech ABRs across free and close-field...
February 6, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28179180/developing-a-3-choice-serial-reaction-time-task-for-examining-neural-and-cognitive-function-in-an-equine-model
#4
Kirsty Roberts, Andrew J Hemmings, Sebastian D McBride, Matthew O Parker
BACKGROUND: Large animal models of human neurological disorders are advantageous compared to rodent models due to their neuroanatomical complexity, longevity and their ability to be maintained in naturalised environments. Some large animal models spontaneously develop behaviours that closely resemble the symptoms of neural and psychiatric disorders. The horse is an example of this; the domestic form of this species consistently develops spontaneous stereotypic behaviours akin to the compulsive and impulsive behaviours observed in human neurological disorders such as Tourette's syndrome...
February 5, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28163066/development-of-a-simple-rapid-and-robust-intrathecal-catheterization-method-in-the-rat
#5
Curt Mazur, Bethany Fitzsimmons, Fredrik Kamme, Brandon Nichols, Berit Powers, Ed Wancewicz
BACKGROUND: The blood brain barrier (BBB) is an impediment to the development of large and highly charged molecules as therapeutics for diseases and injuries of the central nervous system (CNS). Antisense oligonucleotides (ASOs) are large (6000-8000 MW) and highly charged and therefore do not cross the BBB. A method of circumventing the blood brain barrier to test ASOs, and other non-BBB penetrant molecules, as CNS therapeutics is the direct administration of these molecules to the CNS tissue or cerebral spinal fluid...
February 2, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28163065/a-standardized-method-to-measure-brain-shifts-with-decompressive-hemicraniectomy
#6
Askiel Bruno, Abdurrehman Zahran, Nina Paletta, Laith Maali, Fenwick T Nichols, Ramon Figueroa
BACKGROUND: A standardized, reliable, and practical method for measuring decompressive hemicraniectomy (DHC) defects and brain shifts in malignant middle cerebral artery (MCA) territory infarction is needed for reliable comparisons between computed tomography (CT) scans. Such a method could facilitate further studies on the effects of DHC. NEW METHOD: We describe and apply a method for measuring DHC defects and brain shifts on CT scans in 25 patients with malignant MCA territory infarction...
February 2, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28161299/cell-culture-chamber-with-gas-supply-for-prolonged-recording-of-human-neuronal-cells-on-microelectrode-array
#7
Joose Kreutzer, Laura Ylä-Outinen, Antti-Juhana Mäki, Mervi Ristola, Susanna Narkilahti, Pasi Kallio
BACKGROUND: Typically, live cell analyses are performed outside an incubator in an ambient air, where the lack of sufficient CO2 supply results in a fast change of pH and the high evaporation causes concentration drifts in the culture medium. That limits the experiment time for tens of minutes. In many applications, e.g. in neurotoxicity studies, a prolonged measurement of extracellular activity is, however, essential. NEW METHOD: We demonstrate a simple cell culture chamber that enables stable culture conditions during prolonged extracellular recordings on a microelectrode array (MEA) outside an incubator...
February 1, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28161298/correlative-analysis-of-dopaminergic-and-serotonergic-metabolism-across-the-brain-to-study-monoaminergic-function-and-interaction
#8
Françoise Dellu-Hagedorn, Aurélie Fitoussi, Philippe De Deurwaerdère
BACKGROUND: The widespread innervation of dopamine (DA) and serotonin (5-HT) systems in cortical and subcortical regions suggests that their biochemical interactions can occur in multiple regions directly or indirectly via neurobiological networks. NEW METHOD: The present study was aimed at validating a neurochemical approach of monoaminergic function based on inter-individual variability of monoamine tissue contents in various cortical and subcortical areas. We focused on monoamines metabolism and examined correlations within and between these monoaminergic systems in selected regions for the metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and/or homovanillic acid (HVA) and 5-hydroxyindole acetic acid (5-HIAA) alone or with respect to the turnover indexes DOPAC/DA, DOPAC+HVA/DA and 5-HIAA/5-HT...
February 1, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28147250/an-mr-compatible-gyroscope-based-arm-movement-tracking-system
#9
S Iman Shirinbayan, Jochem W Rieger
BACKGROUND: Functional magnetic resonance imaging is well suited to link neural population activation with movement parameters of complex natural arm movements. However, currently existing MR-compatible arm tracking devices are not constructed to measure arm joint movement parameters of unrestricted movements. Therefore, to date most research focuses on simple arm movements or includes very little knowledge about the actual movement kinematics. NEW METHOD: We developed a low cost gyroscope-based arm movement tracking system (GAMTS) that features MR-compatibility...
January 29, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28143748/a-three-dimensional-neural-spheroid-model-for-capillary-like-network-formation
#10
Molly E Boutin, Liana L Kramer, Liane L Livi, Tyler Brown, Christopher Moore, Diane Hoffman-Kim
BACKGROUND: In vitro three-dimensional neural spheroid models have an in vivo-like cell density, and have the potential to reduce animal usage and increase experimental throughput. The aim of this study was to establish a spheroid model to study the formation of capillary-like networks in a three-dimensional environment that incorporates both neuronal and glial cell types, and does not require exogenous vasculogenic growth factors. NEW METHOD: We created self-assembled, scaffold-free cellular spheroids using primary-derived postnatal rodent cortex as a cell source...
January 28, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28137433/an-improved-method-for-growing-neurons-comparison-with-standard-protocols
#11
Diletta Pozzi, Jelena Ban, Federico Iseppon, Vincent Torre
BACKGROUND: Since different culturing parameters - such as media composition or cell density - lead to different experimental results, it is important to define the protocol used for neuronal cultures. The vital role of astrocytes in maintaining homeostasis of neurons - both in vivo and in vitro - is well established: the majority of improved culturing conditions for primary dissociated neuronal cultures rely on astrocytes. NEW METHOD: Our culturing protocol is based on a novel serum-free preparation of astrocyte - conditioned medium (ACM)...
January 27, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28131862/evidence-accumulation-detected-in-bold-signal-using-slow-perceptual-decision-making
#12
Paul M Krueger, Marieke K van Vugt, Patrick Simen, Leigh Nystrom, Philip Holmes, Jonathan D Cohen
BACKGROUND: We assessed whether evidence accumulation could be observed in the BOLD signal during perceptual decision making. This presents a challenge since the hemodynamic response is slow, while perceptual decisions are typically fast. NEW METHOD: Guided by theoretical predictions of the drift diffusion model, we slowed down decisions by penalizing participants for incorrect responses. Second, we distinguished BOLD activity related to stimulus detection (modeled using a boxcar) from activity related to integration (modeled using a ramp) by minimizing the collinearity of GLM regressors...
January 25, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28115187/semi-automatic-3d-morphological-reconstruction-of-neurons-with-densely-branching-morphology-application-to-retinal-aii-amacrine-cells-imaged-with-multi-photon-excitation-microscopy
#13
Bas-Jan Zandt, Are Losnegård, Erlend Hodneland, Margaret Lin Veruki, Arvid Lundervold, Espen Hartveit
BACKGROUND: Accurate reconstruction of the morphology of single neurons is important for morphometric studies and for developing compartmental models. However, manual morphological reconstruction can be extremely time-consuming and error-prone and algorithms for automatic reconstruction can be challenged when applied to neurons with a high density of extensively branching processes. NEW METHOD: We present a procedure for semi-automatic reconstruction specifically adapted for densely branching neurons such as the AII amacrine cell found in mammalian retinas...
January 20, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28109833/a-novel-method-linking-neural-connectivity-to-behavioral-fluctuations-behavior-regressed-connectivity
#14
Antony D Passaro, Jean M Vettel, Jonathan McDaniel, Vernon Lawhern, Piotr J Franaszczuk, Stephen M Gordon
BACKGROUND: During an experimental session, behavioral performance fluctuates, yet most neuroimaging analyses of functional connectivity derive a single connectivity pattern. These conventional connectivity approaches assume that since the underlying behavior of the task remains constant, the connectivity pattern is also constant. NEW METHOD: We introduce a novel method, behavior-regressed connectivity (BRC), to directly examine behavioral fluctuations within an experimental session and capture their relationship to changes in functional connectivity...
January 18, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28109832/a-novel-hybrid-auditory-bci-paradigm-combining-assr-and-p300
#15
Netiwit Kaongoen, Sungho Jo
BACKGROUND: Brain-computer interface (BCI) is a technology that provides an alternative way of communication by translating brain activities into digital commands. Due to the incapability of using the vision-dependent BCI for patients who have visual impairment, auditory stimuli have been used to substitute the conventional visual stimuli. NEW METHOD: This paper introduces a hybrid auditory BCI that utilizes and combines auditory steady state response (ASSR) and spatial-auditory P300 BCI to improve the performance for the auditory BCI system...
January 18, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28104487/comparison-of-a-special-designed-high-intensity-coil-to-a-standard-round-coil-tms-setting
#16
Andrea Szelényi, Julia Wölfle, Igor Fischer, Hans-Jakob Steiger, Michael Sabel
BACKGROUND: Routine diagnostic Transcranial Magnetic Stimulation (TMS) is performed with a round coil (RC) for cranial and spinal root stimulation, being less successful for motor evoked potentials (MEP) of lower limb muscles. MEP elicited with a special configured flat figure-of-eight coil designed for high intensity stimulation (HI-coil) were compared to RC with regard to handling, efficiency, and physiological properties of MEP. NEW METHODS: MEP elicited with HI-coil and 9-cm diameter RC for cortical, spinal and peripheral stimulation (PES) were compared for Motor threshold (MT), latencies and amplitudes of bilateral Abductor pollicis brevis (APB) and Abductor hallucis muscles (AH)...
January 16, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28104486/valid-statistical-approaches-for-analyzing-sholl-data-mixed-effects-versus-simple-linear-models
#17
Machelle D Wilson, Sunjay Sethi, Pamela J Lein, Kimberly P Keil
BACKGROUND: The Sholl technique is widely used to quantify dendritic morphology. Data from such studies, which typically sample multiple neurons per animal, are often analyzed using simple linear models. However, simple linear models fail to account for intra-class correlation that occurs with clustered data, which can lead to faulty inferences. NEW METHOD: Mixed effects models account for intra-class correlation that occurs with clustered data; thus, these models more accurately estimate the standard deviation of the parameter estimate, which produces more accurate p-values...
January 16, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28104485/green-channel-autofluorescence-imaging-a-novel-and-sensitive-technique-to-delineate-infarcts
#18
Kang-Hoon Je, Wi-Sun Ryu, Su-Kyoung Lee, Eo Jin Kim, Jeong-Yeon Kim, Hee Jeong Jang, Jung E Park, Matthias Nahrendorf, Dawid Schellingerhout, Dong-Eog Kim
BACKGROUND: We have found that infarcted brain regions exhibit green channel autofluorescence (GCAF). Here, we compare ex vivo GCAF-imaging with 2,3,5-triphenylteterazolium chloride (TTC)-staining. NEW METHOD: C57BL/6 mice (n=120) underwent GCAF-imaging after transient or permanent middle cerebral artery occlusion (tMCAO or pMCAO). COMPARISON WITH EXISTING METHODS: TTC-staining may not reflect subtle ischemic injury. TTC-stained tissues, when reused, are prone to processing artifacts related to prior TTC-staining...
January 16, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28099872/assessment-of-gait-and-sensorimotor-deficits-in-the-d1ct-7-mouse-model-of-tourette-syndrome
#19
Stephen C Fowler, Laura J Mosher, Sean C Godar, Marco Bortolato
Tourette syndrome (TS) is a neurodevelopmental disorder characterized by multiple motor and phonic tics. While TS patients have been also shown to exhibit subtle abnormalities of sensorimotor integration and gait, animal models of this disorder are seldom tested for these functions. To fill this gap, we assessed gait and sensorimotor integration in the D1CT-7 mouse, one of the best-validated animal models of TS. D1CT-7 mice exhibit spontaneous tic-like manifestations, which, in line with the clinical phenomenology of TS, are markedly exacerbated by environmental stress...
January 15, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28089759/using-pupil-size-and-heart-rate-to-infer-affective-states-during-behavioral-neurophysiology-and-neuropsychology-experiments
#20
Andrew R Mitz, Ravi V Chacko, Philip T Putnam, Peter H Rudebeck, Elisabeth A Murray
BACKGROUND: Nonhuman primates (NHPs) are a valuable research model because of their behavioral, physiological and neuroanatomical similarities to humans. In the absence of language, autonomic activity can provide crucial information about cognitive and affective states during single-unit recording, inactivation and lesion studies. Methods standardized for use in humans are not easily adapted to NHPs and detailed guidance has been lacking. NEW METHOD: We provide guidance for monitoring heart rate and pupil size in the behavioral neurophysiology setting by addressing the methodological issues, pitfalls and solutions for NHP studies...
January 13, 2017: Journal of Neuroscience Methods
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