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https://www.readbyqxmd.com/read/27487564/dihydromyricetin-suppresses-inflammatory-responses-in-vitro-and-in-vivo-through-inhibition-of-ikk%C3%AE-activity-in-macrophages
#1
Rui Wang, Jiang Pi, Xiaohui Su, Juan Liu, Xing Zeng, Ivan Wong, Lufen Huang, Hua Zhou, Jiye Cai, Ting Li, Liang Liu
Dihydromyricetin (DMY) a flavonoid derived from medicinal plant Ampelopsis grossedentata, possesses anti-oxidative and anti-inflammatory effects in vitro, however, the in vivo anti-inflammatory action of DMY remains unknown. In the current study, carrageenan-induced paw edema in rat, an acute inflammation model, and RAW264.7 macrophages activated by LPS were employed to evaluate the anti-inflammatory potency of DMY in vivo and in vitro. Results showed that DMY significantly attenuated rat paw edema induced by carrageenan...
November 2016: Scanning
https://www.readbyqxmd.com/read/27482855/design-of-a-high-bandwidth-tripod-scanner-for-high-speed-atomic-force-microscopy
#2
Chen Yang, Jihong Yan, Maja Dukic, Nahid Hosseini, Jie Zhao, Georg E Fantner
Tip-scanning high-speed atomic force microscopes (HS-AFMs) have several advantages over their sample-scanning counterparts. Firstly, they can be used on samples of almost arbitrary size since the high imaging bandwidth of the system is immune to the added mass of the sample and its holder. Depending on their layouts, they also enable the use of several tip-scanning HS-AFMs in combination. However, the need for tracking the cantilever with the readout laser makes designing tip-scanning HS-AFMs difficult. This often results in a reduced resonance frequency of the HS-AFM scanner, or a complex and large set of precision flexures...
November 2016: Scanning
https://www.readbyqxmd.com/read/27400457/information-or-resolution-which-is-required-from-an-sem-to-study-bulk-inorganic-materials
#3
REVIEW
Q Xing
Significant technological advances in scanning electron microscopy (SEM) have been achieved over the past years. Different SEMs can have significant differences in functionality and performance. This work presents the perspectives on selecting an SEM for research on bulk inorganic materials. Understanding materials demands quantitative composition and orientation information, and informative and interpretable images that reveal subtle differences in chemistry, orientation/structure, topography, and electronic structure...
November 2016: Scanning
https://www.readbyqxmd.com/read/27391259/structural-study-on-the-interactions-of-oxaliplatin-and-linear-dna
#4
Yongpeng Zou, Linhai Biao, Fengjie Xu, Ruisi Liu, Zhiguo Liu, Yujie Fu
Damage to cellular DNA is believed to determine the cytotoxicity of oxaliplatin. However, high resolution structures formed by oxaliplatin and different linear DNA remain unclear. This study characterized, the key structures of different linear DNA in the platination process by UV absorption spectra and atomic force microscopy (AFM). Bathochromic shift and hyperchromicity in UV spectra after addition of oxaliplatin revealed that it can disrupt base stacking of DNA in the platination process. AFM results of different linear DNA indicated that, the platination process can induce DNA change from an extended conformation to the network structure with many kinks and finally to the compact particles, or toroids with increasing the incubation time...
November 2016: Scanning
https://www.readbyqxmd.com/read/27353800/trypan-blue-as-an-affordable-marker-for-automated-live-dead-cell-analysis-in-image-cytometry
#5
Susanne Melzer, Celio Siman Mafra Nunes, Denise Coutinho Endringer, Tadeu Uggere de Andrade, Attila Tarnok, Dominik Lenz
The aim of the present study was to combine image cytometry and trypan blue (TB) exclusion staining for a reproducible high-throughput detection of dead cells, enabling TB as an inexpensive marker, to be affordable for many studies and creating the possibility to combine fluorochromes without or with less spectral overlap. Capillary blood was drawn from a healthy volunteer, red blood cells were lysed and leukocyte cell death was induced. Samples were stained with CD45-FITC, CD14-PE, TB and DAPI, and then analyzed using image cytometry (iCys)...
November 2016: Scanning
https://www.readbyqxmd.com/read/27353661/disulfiram-chelated-with-copper-promotes-apoptosis-in-human-breast-cancer-cells-by-impairing-the-mitochondria-functions
#6
Yaping Yang, Kefan Zhang, Yawei Wang, Mengjia Li, Xiaoxue Sun, Zhihong Liang, Liwei Wang, Lixin Chen, Haifeng Yang, Linyan Zhu
Disulfiram (DSF) has been proved to have broad-spectrum anti-alcoholism effects, and it is also found to show stronger anti-tumor effects after chelating with Cu(2+) to form DSF-Cu complex. In this work, we studied the anti-tumor activity of DSF-Cu in MCF-7 cells by flow cytometry, confocal laser scanning microscope, and atomic force microscopy to clarify the underlying anti-tumor mechanisms. MCF-7 cells were incubated with 50, 100, 150, 200, and 250 nM DSF chelated with 10 µM CuCl2 for 24 h. The results showed that DSF-Cu could induce the accumulation of MCF-7 cells in G2/M phase and apoptosis in a concentration-dependent manner...
November 2016: Scanning
https://www.readbyqxmd.com/read/27302216/brain-early-infarct-detection-using-gamma-correction-extreme-level-eliminating-with-weighting-distribution
#7
V Teh, K S Sim, E K Wong
According to the statistic from World Health Organization (WHO), stroke is one of the major causes of death globally. Computed tomography (CT) scan is one of the main medical diagnosis system used for diagnosis of ischemic stroke. CT scan provides brain images in Digital Imaging and Communication in Medicine (DICOM) format. The presentation of CT brain images is mainly relied on the window setting (window center and window width), which converts an image from DICOM format into normal grayscale format. Nevertheless, the ordinary window parameter could not deliver a proper contrast on CT brain images for ischemic stroke detection...
November 2016: Scanning
https://www.readbyqxmd.com/read/27296240/a-micro-computed-tomography-study-of-the-negotiation-and-anatomical-feature-in-apical-root-canal-of-mandibular-molars
#8
Yi Min, Jing-Zhi Ma, Ya Shen, Gary Shun-Pan Cheung, Yuan Gao
The aim of this study was to investigate the clinical negotiation of various apical anatomic features of the mandibular first molars in a Chinese population using micro-computed tomography (micro-CT). A total of 152 mandibular first molars were scanned with micro-CT at 30 µm resolution. The apical 5 mm of root canal (ARC) was reconstructed three dimensionally and classified. Subsequently, the access cavity was prepared with the ARC anatomy blinded to the operator. The ARC was negotiated with a size 10 K file with or without precurve...
November 2016: Scanning
https://www.readbyqxmd.com/read/27288112/bending-resistance-and-cyclic-fatigue-of-a-new-heat-treated-reciprocating-instrument
#9
Emmanuel João Nogueira Leal Silva, Cristiane Rodrigues, Victor Talarico Leal Vieira, Felipe Gonçalves Belladonna, Gustavo De-Deus, Hélio Pereira Lopes
The current study aimed to evaluate the bending resistance and the cyclic fatigue life of a new heat-treated reciprocating instrument (ProDesign R). Untreated ProDesign R, Reciproc R25, and WaveOne Primary instruments were used as reference instruments for comparison. The bending resistance was performed in ten instruments of each system by using a universal testing machine and a proper apparatus. The cyclic fatigue resistance was tested measuring the number of cycles to failure in an artificial stainless steel canal...
November 2016: Scanning
https://www.readbyqxmd.com/read/27285145/simulations-and-measurements-in-scanning-electron-microscopes-at-low-electron-energy
#10
REVIEW
Christopher G H Walker, Luděk Frank, Ilona Müllerová
The advent of new imaging technologies in Scanning Electron Microscopy (SEM) using low energy (0-2 keV) electrons has brought about new ways to study materials at the nanoscale. It also brings new challenges in terms of understanding electron transport at these energies. In addition, reduction in energy has brought new contrast mechanisms producing images that are sometimes difficult to interpret. This is increasing the push for simulation tools, in particular for low impact energies of electrons. The use of Monte Carlo calculations to simulate the transport of electrons in materials has been undertaken by many authors for several decades...
November 2016: Scanning
https://www.readbyqxmd.com/read/27280953/immunomodulatory-effects-of-polysaccharide-compounds-in-macrophages-revealed-by-high-resolution-afm
#11
Jiang Pi, Yuanyuan Wang, Haiyan Zhu, Hua Jin, Jinhuan Jiang, Fen Yang, Chung Wah Ma, Minghua Hu, Fangli Ma, Huaihong Cai, Jiye Cai
Polysaccharide compounds (PCs), which composed of different kinds of polysaccharides always isolated from different kinds of traditional Chinese medicine, are now attracting more and more attentions due to their strong immunomodulatory activities beyond the corresponding one-component polysaccharides. In this study, we demonstrated for the first time that PCs-1 and PCs-2 had strong immunomodulatory effects on macrophages both in in vitro and in vivo models by atomic force microscopy (AFM). By high resolution AFM imaging, PCs-1 and PCs-2 were found to inhibit LPS induced cell surface particle size and roughness increase in RAW264...
November 2016: Scanning
https://www.readbyqxmd.com/read/27273728/scanning-electron-microscopy-combined-with-image-processing-technique-analysis-of-microstructure-texture-and-tenderness-in-semitendinous-and-gluteus-medius-bovine-muscles
#12
Facundo Pieniazek, Valeria Messina
In this study the effect of freeze drying on the microstructure, texture, and tenderness of Semitendinous and Gluteus Medius bovine muscles were analyzed applying Scanning Electron Microscopy combined with image analysis. Samples were analyzed by Scanning Electron Microscopy at different magnifications (250, 500, and 1,000×). Texture parameters were analyzed by Texture analyzer and by image analysis. Tenderness by Warner-Bratzler shear force. Significant differences (p < 0.05) were obtained for image and instrumental texture features...
November 2016: Scanning
https://www.readbyqxmd.com/read/27254103/unexpected-fe-enriched-compounds-observed-in-mg-ce-alloy-an-atomic-scale-stem-investigation
#13
Jingxu Zheng, Bin Chen
This paper investigates into the unexpected nanosize Fe-enriched particles with defects incorporated, using EDS under STEM mode and atomic-resolution HAADF. The particle that locates between eutectic Mg12 Ce phase and α-Mg phase is identified as CeFe2 Si2 compound. Two types of defects are incorporated: Type-I defect is Ce-Si in a simple cubic lattice; Type-II defect is Ce in a FCC lattice. The interface between the defect-free CeFe2 Si2 and the defects is fully coherent. In addition, another two Ce/Fe/Si-enriched particles are observed, proving that the enrichment of Ce, Fe, and Si is not an accidental phenomenon...
November 2016: Scanning
https://www.readbyqxmd.com/read/27253888/autoregressive-linear-least-square-single-scanning-electron-microscope-image-signal-to-noise-ratio-estimation
#14
Kok Swee Sim, Syafiq NorHisham
A technique based on linear Least Squares Regression (LSR) model is applied to estimate signal-to-noise ratio (SNR) of scanning electron microscope (SEM) images. In order to test the accuracy of this technique on SNR estimation, a number of SEM images are initially corrupted with white noise. The autocorrelation function (ACF) of the original and the corrupted SEM images are formed to serve as the reference point to estimate the SNR value of the corrupted image. The LSR technique is then compared with the previous three existing techniques known as nearest neighbourhood, first-order interpolation, and the combination of both nearest neighborhood and first-order interpolation...
November 2016: Scanning
https://www.readbyqxmd.com/read/27220904/the-effect-of-different-beverages-on-the-color-and-translucency-of-flowable-composites
#15
Muhammet Karadas
This study examined the changes in color and translucency of flowable composites after immersion in different beverages. Thirty composite samples were prepared from four flowable composites (G-aenial Universal Flo, Filtek Ultimate, Esthelite Flow Quick, and Clearfil Majesty ES Flow) and a microhybrid composite (Filtek Z-250) and stored in distilled water at 37°C for 24 h. The samples were randomly divided into seven groups and then immersed in different beverages (Red Bull, coffee, black tea, Pepsi Cola, orange juice, and distilled water) for 7 days...
November 2016: Scanning
https://www.readbyqxmd.com/read/27186786/n-acetylcysteine-eradicates-pseudomonas-aeruginosa-biofilms-in-bone-cement
#16
Mehmet Emin Onger, Hasan Gocer, Dilek Emir, Suleyman Kaplan
Biofilm is an example of bacterial group behavior. We investigated the effect of N-acetylcysteine (NAC) alone and in combination with ciprofloxacin on Pseudomonas aeruginosa biofilm formation. Four groups (each contains six molds) of standardized bone cement molds were infected. NAC, ciprofloxacin each alone, and NAC/ciprofloxacin combination were evaluated in point of inhibiting and eradicating biofilm capacity using microbiological and electron microscopical evaluation techniques. Microbial counts and electron microscopical observations showed that the effect of NAC and ciprofloxacin combination on biofilm formation in bone cement is valuable...
November 2016: Scanning
https://www.readbyqxmd.com/read/27171920/sem-and-tem-for-structure-and-properties-characterization-of-bacterial-cellulose-hydroxyapatite-composites
#17
Natalia A Arkharova, Elena I Suvorova, Alexander V Severin, Albert K Khripunov, Sergey V Krasheninnikov, Vera V Klechkovskaya
Preparation of composites with different properties and gradient of components is aimed at better performance of materials for bone substitution. Bacterial cellulose-hydroxyapatite (BC-HAP) composites with various mass ratio of the components (BC-25HAP, BC-4HAP, and BC-HAP) were prepared by a novel method of growing HAP nanocrystals (the linear size ≤30 nm) in water solutions in the presence of the BC gel-film micro-fragments. Varying the BC-HAP ratios leads to a gradual change of the physical properties of the materials...
November 2016: Scanning
https://www.readbyqxmd.com/read/27162104/multi-port-driven-birdcage-coil-for-multiple-mouse-mr-imaging-at-7-t
#18
Phil Heo, Jeung-Hoon Seo, Sang-Doc Han, Yeunchul Ryu, Jong-Deok Byun, Kyoung-Nam Kim, Jung Hee Lee
In ultra-high field (UHF) imaging environments, it has been demonstrated that multiple-mouse magnetic resonance imaging (MM-MRI) is dependent on key factors such as the radiofrequency (RF) coil hardware, imaging protocol, and experimental setup for obtaining high-resolution MR images. A key aspect is the RF coil, and a number of MM-MRI studies have investigated the application of single-channel RF transmit (Tx)/receive (Rx) coils or multi-channel phased array (PA) coil configurations under a single gradient coil set...
November 2016: Scanning
https://www.readbyqxmd.com/read/27120132/atomic-scale-characterization-of-the-equilibrium-%C3%AE-phase-in-mg-nd-y-alloy-by-means-of-haadf-stem
#19
Jingxu Zheng, Bin Chen
The β phase in Mg-Nd-Y alloy, which forms during isothermal ageing, is proved to be Mg41 Nd5 by energy-dispersive X-ray spectroscopy (EDS) results as compositional evidence and by atomic-scaled high angle annular dark field-scanning transmission electron microscopy (HAADF-STEM) direct images from [001]β , [111¯]β, and [3¯11]β as structural evidence. SCANNING 38:743-746, 2016. © 2016 Wiley Periodicals, Inc.
November 2016: Scanning
https://www.readbyqxmd.com/read/27111226/nanostructural-characterization-of-sf9-cells-during-virus-like-particles-generation
#20
Gi-Ja Lee, Su-Hwa Lee, Young Ju Lee, Fu-Shi Quan
Sf9 cells (a clonal isolate of Spodoptera frugiperda Sf21 cells) are commonly used to generate recombinant virus-like particles (VLPs). For VLPs generation, Sf9 cells are infected with recombinant baculoviruses (rBV) expressing desired proteins. During rBV infections, Sf9 cells have changes in cell diameters and surface structures. In this study, for the first time, we investigated nanostructural changes of Sf9 cells using atomic force microscopy (AFM) during VLPs generation containing Toxoplasma gondii rhoptry protein 18 (ROP 18)...
November 2016: Scanning
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