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Comparative Immunology, Microbiology and Infectious Diseases

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https://www.readbyqxmd.com/read/28750869/probiotic-lactobacillus-acidophilus-bacteria-or-synthetic-tlr2-agonist-boost-the-growth-of-chicken-embryo-intestinal-organoids-in-cultures-comprising-epithelial-cells-and-myofibroblasts
#1
Malgorzata Pierzchalska, Malgorzata Panek, Malgorzata Czyrnek, Anna Gielicz, Barbara Mickowska, Maja Grabacka
The intestinal epithelial cells reside in close proximity to myofibroblasts and microbiota, which are supposed to have an impact on intestinal stem cells fate and to influence processes of tissue maturation and regeneration. Mechanism underlying these phenomena and their diversity among vertebrates can be studied in 3D organoid cultures. We investigated the growth of chicken embryo intestinal epithelial organoids in Matrigel with and without Toll-like receptors (TLRs) stimulation. The organoid cultures contained also some myofibroblasts with potential to promote intestinal stem cell survival...
August 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28750868/effect-of-colostrum-quantity-and-quality-on-neonatal-calf-diarrhoea-due-to-cryptosporidium-spp-infection
#2
K Arsenopoulos, A Theodoridis, E Papadopoulos
This study was conducted to assess the effect of colostrum quality and quantity on Cryptosporidium spp. calf diarrhoea in an intensive dairy cattle farm in Greece. Faecal samples were collected from 100 dairy calves randomly selected and born during all 4 seasons (March 2015 to May 2016) of the year. In total, 71% of the selected calves were positive for Cryptosporidium spp. oocysts. The statistical analysis revealed influence of colostrum quality on faecal score. Linear regression showed that the colostrum quantity during the first day of life was negatively associated with the number of Cryptosporidium spp...
August 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28750867/west-nile-virus-infection-in-horses-indian-ocean
#3
E Cardinale, C Bernard, S Lecollinet, V M Rakotoharinome, J Ravaomanana, M Roger, M M Olive, D Meenowa, M R Jaumally, J Melanie, J M Héraud, S Zientara, C Cêtre-Sossah
The circulation of West Nile virus (WNV) in horses was investigated in the Southwest Indian ocean. In 2010, blood samples were collected from a total of 303 horses originating from Madagascar, Mauritius, Reunion and the Seychelles and tested for WNV-specific antibodies. An overall seroprevalence of 27.39% was detected in the Indian Ocean with the highest WNV antibody prevalence of 46.22% (95% CI: [37.4-55.2%]) in Madagascar. The age and origin of the horses were found to be associated with the WNV infection risk...
August 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28750866/two-novel-ehrlichia-strains-detected-in-amblyomma-tigrinum-ticks-associated-to-dogs-in-peri-urban-areas-of-argentina
#4
Gabriel L Cicuttin, M Nazarena De Salvo, Santiago Nava
The aim of this work was to describe two novel strains of Ehrlichia associated to Amblyomma tigrinum from Argentina. Molecular detection of agents belonging to the family Anaplasmataceae was performed targeting three different loci: 16S rRNA gene, dsb gene and a fragment of groESL heat shock operon. The results have shown that two different strains of Ehrlichia sp. associated to A. tigrinum are circulating in peri-urban areas of Argentina. The Ehrlichia strain detected in ticks from San Luis Province, named as Ehrlichia sp...
August 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28750865/human-head-lice-and-pubic-lice-reveal-the-presence-of-several-acinetobacter-species-in-algiers-algeria
#5
Nassima Mana, Meriem Louni, Philippe Parola, Idir Bitam
There are two majorspecies of medically important lice that parasitize humans: Phthirus pubis, found in pubic hair, and Pediculus humanus. Pediculus humanus consists of two eco types that live in specific niches on the human host: body lice (Pediculus humanus humanus), found on the human body and clothing, and head lice (Pediculus humanus capitis), found on the scalp. To date, only body lice are known to be vectors of human disease; however, it has recently been reported that the DNA of several bacterial agents has been detected in head lice, raising questions about their role in the transmission of pathogens...
August 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28750864/identification-of-a-new-diagnostic-antigen-for-glanders-using-immunoproteome-analysis
#6
Sudhir K Dohre, Aayushi Kamthan, Sandeep Singh, Syed Imteyaz Alam, Subodh Kumar
Glanders is a disease of horses, donkeys and mules. The causative agent Burkholderia mallei, is a biorisk group 3 pathogen and is also a biothreat agent. Simple and rapid diagnostic tool is essential for control of glanders. Using a proteomic approach and immunoblotting with equine sera, we identified 12 protein antigens that may have diagnostic potential. Various immunoreactive proteins e.g. GroEL, translation elongation factor Tu, elongation factor Ts, arginine deiminase, malate dehydrogenase, DNA directed RNA polymerase subunit alpha were identified on 2-dimentional immunoblots...
August 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28750863/microscopic-and-molecular-identification-of-hemotropic-mycoplasmas-in-south-american-coatis-nasua-nasua
#7
Michelle P Cubilla, Leonilda C Santos, Wanderlei de Moraes, Zalmir S Cubas, Christian M Leutenegger, Marko Estrada, LeAnn L Lindsay, Edvaldo S Trindade, Célia Regina C Franco, Rafael F C Vieira, Alexander W Biondo, Jane E Sykes
Hemoplasmas were detected in two apparently healthy captive South American coatis (Nasua nasua) from southern Brazil during an investigation for vector-borne pathogens. Blood was subjected to packed cell volume (PCV) determination, a commercial real-time PCR panel for the detection of Anaplasma spp., Babesia spp., Bartonella spp., Hepatozoon spp., Leishmania spp., Mycoplasma haemofelis, 'Candidatus Mycoplasma turicensis', 'Candidatus Mycoplasma haemominutum', Neorickettsia risticii, Rickettsia rickettsii and Leptospira spp...
August 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28750862/alteration-of-haemostatic-parameters-in-uncomplicated-canine-babesiosis
#8
Josipa Kuleš, Jelena Gotić, Vladimir Mrljak, Renata Barić Rafaj
Babesiosis is a tick-borne zoonotic disease caused by haemoprotozoan parasites. The aim of this study were to assess markers of coagulation pathways in 25 dogs with naturally occurring babesiosis caused by B. canis, compared to 10 healthy controls. Protein C (PC) and antithrombin III (AT III) activity were assessed using a chromogenic substrate test, while levels of thrombin-antithrombin (TAT) complexes, activated protein C (APC) and endothelial protein C receptor were assessed using canine-specific ELISA. AT III activity was decreased as a result of a negative acute phase response, degradation by elastase, reduced availability of glycosaminoglycans, and, most importantly, consumption as a consequence of thrombin formation...
August 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28673463/occurrence-of-hemotropic-mycoplasmas-in-non-human-primates-alouatta-caraya-sapajus-nigritus-and-callithrix-jacchus-of-southern-brazil
#9
Michelle P Cubilla, Leonilda C Santos, Wanderlei de Moraes, Zalmir S Cubas, Christian M Leutenegger, Marko Estrada, Rafael F C Vieira, Maurilio J Soares, LeAnn L Lindsay, Jane E Sykes, Alexander W Biondo
Hemoplasmas, the erythrocyte-associated mycoplasmas, have been detected in several primates, causing mostly subclinical infection. This study aimed to determine the prevalence of hemoplasma infection in captive and free-ranging monkeys from southern Brazil, as well as factors and hematological abnormalities associated with infection. Blood samples from 40 non-human primates (NHP) were tested for hemoplasmas and coinfections. An overall of 10/40 (25.0%) NHP tested positive for hemoplasmas using PCR-based assays, including 9/14 (64...
June 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28673462/cell-free-dna-induced-alteration-of-autophagy-response-and-tlr9-signaling-their-relation-to-amelioration-of-dss-colitis
#10
Györgyi Műzes, Anna L Kiss, Zsolt Tulassay, Ferenc Sipos
BACKGROUND: The influence of cell-free DNA (fDNA) administration on the TLR9-autophagy regulatory crosstalk within inflammatory circumstances remains unclear. AIMS: To examine the immunobiologic effects of iv. fDNA injection on the TLR9-mediated autophagy response in murine DSS-colitis. METHODS: Different types of modified fDNAs were administered to DSS-colitic mice. Disease and histological activities, spleen index were measured. Changes of the TLR9-associated and autophagy-related gene expression profiles of lamina proprial cells and splenocytes were assayed by quantitative real-time PCR, and validated by immunohistochemistries...
June 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28673461/experimental-infection-of-artibeus-intermedius-with-a-vampire-bat-rabies-virus
#11
Cirani Obregón-Morales, Álvaro Aguilar-Setién, Leonardo Perea Martínez, Guillermo Galvez-Romero, Flor Olivia Martínez-Martínez, Nidia Aréchiga-Ceballos
Experimental infection of Artibeus intermedius, the great fruit-eating bat, was performed with vampire bat rabies isolates. Bats (n=35) were captured in the wild and quarantined prior to experimental infection. No rabies antibodies were detected by rapid fluorescent focus inhibition test (RFFIT) prior to infection. Three doses of rabies virus (RV) and three different routes of infection were used. One out of 35 bats died without showing any clinical signs at day 14 and was positive for rabies. None of the 34 other bats showed clinical signs for rabies, but high antibody titers were detected post-inoculation, suggesting either innate immune response to the vampire bat rabies virus or possible pre-exposure to RV and inoculation leading to a booster effect...
June 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28673460/a-survey-of-canine-haemoprotozoan-parasites-from-turkey-including-molecular-evidence-of-an-unnamed-babesia
#12
Munir Aktas, Sezayi Ozubek
Canine tick-borne apicomplexan parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. A reverse line blot assay was performed on 219 blood samples collected from domestic dogs for simultaneous detection of all named canine piroplasm species as well as Hepatozoon canis. Ten samples hybridized to the Theileria/Babesia and Babesia catch all probes but did not hybridize to any species-specific probe tested, suggesting the presence of an unrecognized Babesia species or genotype...
June 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28673459/serological-survey-of-neospora-caninum-and-toxoplasma-gondii-in-cattle-bos-indicus-and-water-buffaloes-bubalus-bubalis-in-ten-provinces-of-brazil
#13
Jenevaldo Barbosa da Silva, Rafael Romero Nicolino, Gisele Maria Fagundes, Henrique Dos Anjos Bomjardim, Alessandra Dos Santos Belo Reis, Danillo Henrique da Silva Lima, Carlos Magno Chaves Oliveira, José Diomedes Barbosa, Adivaldo Henrique da Fonseca
The objective of this study was to determine the prevalence of antibodies to Neospora caninum and Toxoplasma gondii among 500 cattle (Bos indicus) and 500 buffaloes (Bubalus bubalis) using the indirect fluorescent antibody test (IFAT) technique. Blood samples from were collected from water buffalo and cattle in 10 municipalities in the northern region of Brazil. The frequency of cattle and water buffaloes seropositive for Neospora caninum in Pará state, Brazil, was 55% and 44%, respectively, and the frequency of cattle and water buffaloes seropositive for Toxoplasma gondii was 52% and 39%, respectively...
June 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28673458/performance-endoparasitary-control-and-blood-values-of-ewes-locally-adapted-in-semiarid-region
#14
Josiel Borges Ferreira, Ana Carla Diógenes Suassuna Bezerra, Magda Maria Guilhermino, Jacinara Hody Gurgel Morais Leite, Wilma Emanuela da Silva, Renato Diógenes Macedo Paiva, Tallysson Nogueira Barbosa, José Ernandes Rufino de Sousa, Débora Andréa Evangelista Façanha
This study evaluated the variation in the prevalence of endoparasitoses and their impact on body condition and blood values of sheep of the Morada Nova breed. A total of 138 ewes were examined for their morphology (body weight, BW; body condition score, BCS), parasitology (faecal egg count, FEC; Famacha© score; coproculture), hematology (red blood cell count,RBC); hemoglobin concentration, HE; packet cell volume, PCV; mean corpuscular volume, MCV,; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; leukocytes, WBC) and serum biochemistry (glucose, cholesterol, triglycerides, urea, creatinine, total protein, albumin, globulin, AST and ALT)...
June 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28673457/incidence-and-vertical-transmission-rate-of-neospora-caninum-in-sheep
#15
Paulo César G A Filho, Júnior M B Oliveira, Muller R Andrade, José G Silva, Pomy C P Kim, Jonatas C Almeida, Wagnner J N Porto, Rinaldo A Mota
The infection by Neospora caninum in sheep can lead to abortion and the birth of weak and debilitated lambs. The aim of this study was to assess the incidence of natural infection by Neospora caninum and the vertical transmission rate among sheep. A flock of 50 sheep was monitored for serum antibody titres against N. caninum and seroconversion over a period of six months using an indirect ELISA technique. The offspring of the herd was also investigated regarding anti-N. caninum antibodies to determine the vertical transmission rate through the indirect immunofluorescence technique...
June 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28673456/pathogenic-leptospira-spp-in-bats-molecular-investigation-in-southern-brazil
#16
Fabiana Quoos Mayer, Emily Marques Dos Reis, André Vinícius Andrade Bezerra, Cristine Cerva, Júlio Rosa, Samuel Paulo Cibulski, Francisco Esmaile Sales Lima, Susi Missel Pacheco, Rogério Oliveira Rodrigues
The present study aimed to investigate the frequency of pathogenic Leptospira spp. in Brazilian bats and to determine possible risk factors associated to it. Ninety two bats of 12 species were evaluated. Whole genomic DNA from kidneys was extracted and real-time PCR specific to pathogenic Leptospira spp. was applied. Association between the frequency of specimens positive for Leptospira spp. and sex, age, bat species or family, season of collection, geographic localization and feeding habits was evaluated. The results showed that 39...
June 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28673455/neorickettsia-risticii-rickettsia-sp-and-bartonella-sp-in-tadarida-brasiliensis-bats-from-buenos-aires-argentina
#17
Gabriel L Cicuttin, María N De Salvo, Isabel La Rosa, Federico E Gury Dohmen
Bats are potential reservoirs of many vector-borne bacterial pathogens. The aim of the present study was to detect species of Anaplasma, Ehrlichia, Neorickettsia, Rickettsia, Borrelia and Bartonella in Brazilian free-tailed bats (Tadarida brasiliensis, Molossidae) from Buenos Aires city, Argentina. Between 2012 and 2013, 61 T. brasiliensis from urban areas of Buenos Aires city were studied. The samples were molecularly screened by PCR and sequencing. Five bats (8.2%) were positive to Neorickettsia risticii, one (1...
June 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28522284/corrigendum-to-development-of-a-new-pcr-based-assay-to-detect-anaplasmataceae-and-the-first-report-of-anaplasma-phagocytophilum-and-anaplasma-platys-in-cattle-from-algeria-comp-immunol-microbiol-infect-dis-4-39-2015-39-45-elsevier-ltd
#18
Mustapha Dahmani, Bernard Davoust, Mohamed Seghir Benterki, Florence Fenollar, Didier Raoult, Oleg Mediannikov
No abstract text is available yet for this article.
May 15, 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28504100/ectonucleotidase-and-adenosine-deaminase-as-inflammatory-marker-in-dairy-cows-naturally-infected-by-dictyocaulus-viviparus
#19
Aleksandro S da Silva, Matheus D Baldissera, Nathieli B Bottari, Pedro H Doleski, Guilherme M Carmo, Claiton I Schwertz, Neuber J Lucca, Luan C Henker, Manoela M Piva, Patrícia Giacomin, Lenita M Stefani, Daniela B R Leal, Gustavo Machado, Ricardo E Mendes
The aim of this study was to evaluate the influence of dictyocaulosis (mild or severe) on enzymes of NTPDase, 5'-nucleotidase, and adenosine deaminase (ADA) of dairy cows naturally infected by Dictyocaulus viviparus. Blood and faeces were collected from 22 dairy cows of the same farm to evaluate NTPDase (ATP and ADP substrate), 5'-nucleotidase, and ADA activities on days 0 (pre-treatment) and 10 (post-treatment). Seric activities of NTPDase (ATP substrate), 5'-nucleotidase, and ADA were lower (P<0.05) in D...
April 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28504099/zoonotic-intestinal-parasites-and-vector-borne-pathogens-in-italian-shelter-and-kennel-dogs
#20
Donato Traversa, Angela Di Cesare, Giulia Simonato, Rudi Cassini, Carmine Merola, Anastasia Diakou, Lénaïg Halos, Frederic Beugnet, Antonio Frangipane di Regalbono
This study investigated the presence of zoonotic parasites and vector-borne pathogens in dogs housed in kennels and shelters from four sites of Italy. A total of 150 adoptable dogs was examined with different microscopic, serological and molecular methods. Overall 129 dogs (86%) were positive for one or more parasites and/or pathogens transmitted by ectoparasites. Forty-eight (32%) were positive for one infection, while 81 (54%) for more than one pathogen. The most common zoonotic helminths recorded were hookworms, roundworms and Capillaria aerophila, followed by mosquito-borne Dirofilaria spp...
April 2017: Comparative Immunology, Microbiology and Infectious Diseases
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