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Current Microbiology

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https://www.readbyqxmd.com/read/29987521/a-dual-replicon-shuttle-vector-system-for-heterologous-gene-expression-in-a-broad-range-of-gram-positive-and-gram-negative-bacteria
#1
Mingxi Hua, Jingjing Guo, Min Li, Chen Chen, Yuanyuan Zhang, Chuan Song, Dong Jiang, Pengcheng Du, Hui Zeng
Origin of replication (ori in theta-replicating plasmids or dso in rolling circle replicating plasmids) initiates plasmid replication in a broad range of bacteria. These two kinds of plasmids were both identified in Streptococcus, a genus composed of both human commensal bacteria and pathogens with the ability to cause severe community-acquired infections, including meningitides, septicemia, and respiratory tract diseases. Given the important roles of Streptococcus in the exchange of genetic elements with other symbiotic microbes, the genotypes and phenotypes of both Streptococcus spp...
July 9, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29982935/anaerococcus-jeddahensis-sp-nov-a-new-bacterial-species-isolated-from-healthy-nomadic-bedouin-woman-from-saudi-arabia
#2
Niokhor Dione, Sara Bellali, Muhammad Yasir, Esam I Azhar, Fehmida Bibi, Mamadou Beye, Nicholas Armstrong, Frédéric Cadoret, Asif A Jiman-Fatani, Nayel Helmy, Jaishriram Rathored, Noémie Labas, Pierre-Edouard Fournier, Didier Raoult, Jean-Christophe Lagier
An understanding of the microbial diversity of the human body has generated significant interest in recent years. With the advent of MALDI-TOF mass spectrometry, high-speed sequencing, and the rebirth of microbial culture, knowledge of human microbiota is growing. Using culturomics, a strategy to explore the microbial diversity of samples, coupled with a taxono-genomic strategy, we isolated a new bacterium named Anaerococcus jeddahensis sp. nov. strain SB3T . This strain was isolated from the stool sample of a healthy nomadic Bedouin woman from Saudi Arabia...
July 7, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29980814/regulation-of-iaa-biosynthesis-in-azospirillum-brasilense-under-environmental-stress-conditions
#3
Romina Molina, Diego Rivera, Verónica Mora, Gastón López, Susana Rosas, Stijn Spaepen, Jos Vanderleyden, Fabricio Cassán
Indole-3-acetic acid (IAA) is one of the most important molecules produced by Azospirillum sp., given that it affects plant growth and development. Azospirillum brasilense strains Sp245 and Az39 (pFAJ64) were pre-incubated in MMAB medium plus 100 mg/mL L-tryptophan and treated with or exposed to the following (a) abiotic and (b) biotic stress effectors: (a) 100 mM NaCl or Na2 SO4, 4.0% (w/v) PEG6000, 0.5 mM H2 O2, 0.1 mM abscisic acid, 0.1 mM 1-aminocyclopropane 1-carboxylic acid, 45 °C or daylight, and (b) 4...
July 6, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29980813/actinomadura-hankyongense-sp-nov-isolated-from-soil-of-ginseng-cultivating-field
#4
Muhammad Zubair Siddiqi, Qingmei Liu, Kang Duk Choi, Soon Youl Lee, Jae Hag Lee, Wan Taek Im
A Gram-positive, rod-shaped, non-spore-forming, and aerobic bacterium (Gsoil 556T ) was isolated from soil of a ginseng field and subjected to its taxonomic position. Based on 16S rRNA gene sequence similarity, strain Gsoil 556T was shown to belong to the genus Actinomadura of the family Thermomonosporaceae and was closely related to A. montaniterrae CYP1-1BT (99.3%), A. nitritigenes DSM 44137T (98.7%), and A. rudentiformis HMC1T (98.5%), while it showed less than 98.4% sequence similarity to the other species of this genus...
July 6, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29980812/evaluation-of-legiolert-for-quantification-of-legionella-pneumophila-from-non-potable-water
#5
Melanie M Rech, Brian M Swalla, Jason K Dobranic
Legiolert® is a new culture method for quantification of Legionella pneumophila, which is the primary species associated with Legionnaires' disease. The test is based on a most probable number approach, and differs significantly from traditional culture methods by providing results at 7 days, rapid sample preparation and analysis, and objective interpretation of test results. In this study, we compared the performance of Legiolert with the U.S. Centers for Disease Control and Prevention (CDC) method for detection of L...
July 6, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29946896/streptomyces-dioscori-sp-nov-a-novel-endophytic-actinobacterium-isolated-from-bulbil-of-dioscorea-bulbifera-l
#6
Zhikuan Wang, Jiewei Tian, Xiaoguang Li, Longzhan Gan, Linling He, Yiwen Chu, Yongqiang Tian
A novel endophytic actinobacterium strain, A217T , was isolated from the bulbil of Dioscorea bulbifera L. Its taxonomic position was characterized using a polyphasic study. Morphological and chemotaxonomic characteristics of strain A217T were consistent with those of members of the genus Streptomyces: long straight to flexuous spore chain; cellular components contained LL-diaminopimelic acid, ribose, and small traces of glucose in whole-cell hydrolysates; MK-9(H6 ) and MK-9(H8 ) as predominant menaquinones...
June 26, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29934881/streptococcus-mutans-in-umbilical-cord-blood-peripheral-blood-and-saliva-from-healthy-mothers
#7
Marcelly Milhomem Mendes, Camilla Beatriz da Silva, Denise Bertulucci Rocha Rodrigues, Barbara Rocha Rodrigues, Vinicius Rangel Geraldo-Martins, Virginia Paes Leme Ferriani, Virmondes Rodrigues, Ruchele Dias Nogueira
The aims of this study were to analyze the presence of Streptococcus mutans (SM)-DNA in cord blood (CB), maternal peripheral blood (PB), and maternal saliva (SA) and compare with data collected in health surveys. Sixty-four healthy women with pregnancies to term and without complications attending for elective cesarean section in the Clinical Hospital of Ribeirao Preto, Sao Paulo were included. Samples of PB and unstimulated SA were obtained on the day of hospitalization and samples of CB were collected after the delivery section...
June 22, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29931384/complete-genome-sequence-of-bacillus-sp-sj-10-kccm-90078-producing-400-kda-poly-%C3%AE-glutamic-acid
#8
Won Je Jang, Jong Min Lee, Yu-Ri Kim, Md Tawheed Hasan, In-Soo Kong
Bacillus sp. SJ-10 (KCCM 90078, JCM 15709) is a halotolerant bacterium isolated from a traditional Korean food, i.e., salt-fermented fish (jeotgal). The bacterium can survive and engage in metabolism at high salt concentrations. Here, we reported complete genome sequence of Bacillus sp. SJ-10, which has a single circular chromosome of 4,041,649 base pairs with a guanine-cytosine content of 46.39%. Bacillus sp. SJ-10 encodes a subunit of poly-γ-glutamic acid (γ-PGA) with a molecular weight of approximately 400 kDa, which contains four γ-PGA synthases (pgsB, pgsC, pgsAA and pgsE) and one γ-PGA-releasing gene (pgsS)...
June 21, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29922971/surface-display-of-heterologous-%C3%AE-galactosidase-in-food-grade-recombinant-lactococcus-lactis
#9
Supeng Yin, Hongbin Zhu, Mengyu Shen, Gang Li, Shuguang Lu, Yan Zhao, Shuai Le, Yinling Tan, Yizhi Peng, Fuquan Hu, Jing Wang
β-Galactosidase is an essential enzyme for the metabolism of lactose in human beings and has an important role in the treatment of lactose intolerance (LI). β-Galactosidase expressed by intestinal microflora, such as lactic acid bacteria (LAB), also alleviates LI. A promising approach to LI management is to exploit a food-grade LAB delivery system that can inhabit the human intestine and overproduce β-galactosidase. In this study, we constructed a food-grade β-galactosidase surface display delivery system and then integrated into the chromosome of Lactococcus lactis (L...
June 19, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29922970/fecal-bacteriome-and-mycobiome-in-bats-with-diverse-diets-in-south-china
#10
Juan Li, Linmiao Li, Haiying Jiang, Lihong Yuan, Libiao Zhang, Jing-E Ma, Xiujuan Zhang, Minhua Cheng, Jinping Chen
Bats can be divided into frugivory, nectarivory, insectivory, and sanguivory based on their diets, and are therefore ideal wild animal models to study the relationship between diets and intestinal microflora. Early studies of bat gut bacteria showed that the diversity and structure of intestinal bacterial communities in bats are closely related to dietary changes. Worthy of note, intestinal microbes are composed of bacteria, fungi, protozoa, and archaea. Although the number of gut fungi is much lower than that of gut bacteria, they also play an important role in maintaining the host homeostasis...
June 19, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29922969/adpa-sd-a-positive-regulator-for-morphological-development-and-toyocamycin-biosynthesis-in-streptomyces-diastatochromogenes-1628
#11
Juan Wang, Jie Xu, Shuai Luo, Zheng Ma, Andreas Bechthold, Xiaoping Yu
AdpA is studied and considered as a pleiotropic regulator which is involved in morphological development and secondary metabolism in many Streptomyces. In this study, AdpAsd, which was cloned from toyocamycin (TM)-producing strain Streptomyces diastatochromogenes 1628, was identified as an ortholog of AdpA and belongs to a large subfamily of the AraC/XylS family. In order to elucidate the correlation of AdpAsd with TM biosynthesis and morphological differentiation, adpA sd was placed under the control of the ermE* promoter in plasmid pIB139...
June 19, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29916034/differences-in-carbohydrates-utilization-and-antibiotic-resistance-between-streptococcus-macedonicus-and-streptococcus-thermophilus-strains-isolated-from-dairy-products-in-italy
#12
Armin Tarrah, Laura Treu, Sabrina Giaretta, Vinicius Duarte, Viviana Corich, Alessio Giacomini
Streptococcus thermophilus and S. macedonicus are the only two species of the genus related to food productions so far known. In the present study, eight S. thermophilus and seven S. macedonicus strains isolated from dairy environments in Italy were compared in order to evidence possible species-specific technological characteristics. Their capability to use lactose, galactose, fructose, and glucose, sugars commonly present in foods and two carbohydrates considered as prebiotics, xylose and inulin, along with the respective growth kinetics were studied...
June 18, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29907938/analysis-of-the-gut-microbial-diversity-of-dairy-cows-during-peak-lactation-by-pacbio-single-molecule-real-time-smrt-sequencing
#13
Weicheng Li, Qiangchuan Hou, Yanjie Wang, Huimin Ma, Yahua Liu, Feiyan Zhao, Jing Li, Lai-Yu Kwok, Jie Yu, Zhihong Sun, Tiansong Sun
BACKGROUND: The gut microbes of dairy cows are strongly associated with their health, but the relationship between milk production and the intestinal microbiota has seldom been studied. Thus, we explored the diversity of the intestinal microbiota during peak lactation of dairy cows. METHODS: The intestinal microbiota of nine dairy cows at peak lactation was evaluated using the Pacific Biosciences single-molecule real-time (PacBio SMRT) sequencing approach. RESULTS: A total of 32,670 high-quality 16S rRNA gene sequences were obtained, belonging to 12 phyla, 59 families, 107 genera, and 162 species...
June 15, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29948009/generation-and-in-vivo-characterization-of-tn5-induced-biofilm-mutants-of-vibrio-cholerae-o139
#14
Preeti Gupta, Bharti Mankere, Shami Chekkoora Keloth, Urmil Tuteja, Kulanthaivel Thava Chelvam
The Gram-negative bacterium Vibrio cholerae is a unique pathogen with an ability to colonize human intestine as well as outside environments. The biofilm, an organized polymeric structure produced by this bacterium known to be a significant factor for the survival and persistence in hostile conditions. However, the direct role of biofilm formation by this bacterium in environmental persistence, in vivo colonization, and pathogenesis remains unexplored. In this study, we have generated biofilm-altered Tn5 mutants of V...
June 12, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29948008/paenibacillus-maysiensis-sp-nov-a-nitrogen-fixing-species-isolated-from-the-rhizosphere-soil-of-maize
#15
Tian-Shu Wang, Jiu-Yan Xie, Li-Ying Wang, San-Feng Chen
A novel bacterium SX-49T with nitrogen-fixing capability was isolated from the rhizosphere soil of maize. Phylogenetic analysis of nifH gene fragment and 16S rRNA gene sequence revealed that the strain SX-49T is a member of the genus Paenibacillus. Values of 16S rRNA gene sequence similarity were highest between SX-49T and P. jamilae DSM 13815T (97.0%), P. brasiliensis DSM 14914T (97.8%), P. polymyxa DSM 36T (97.5%), and P. terrae DSM 15891T (98.8%). The similarity between SX-49T and other Paenibacillus species was < 97...
June 8, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29872901/sphingomonas-montis-sp-nov-isolated-from-forest-soil-of-low-altitude-mountain
#16
Dhiraj Kumar Chaudhary, Ram Hari Dahal, Jaisoo Kim
Strain DRJ-4T was isolated from forest soil of a low-altitude mountain and was taxonomically characterized by a polyphasic approach. This strain was yellow-colored, Gram-stain-negative, strictly aerobic, motile, non-sporulating, catalase-positive, oxidase-negative, and rod-shaped. Strain DRJ-4T was able to grow at 20-42 °C, pH 6.5-10.0, and 0-1.0% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain DRJ-4T formed a distinct lineage within the members of the family Sphingomonadaceae of the phylum Proteobacteria and moderately related to Sphingomonas faucium E62-3T (96...
June 5, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29869679/species-diversity-and-functional-prediction-of-surface-bacterial-communities-on-aging-flue-cured-tobaccos
#17
Fan Wang, Hongwei Zhao, Haiying Xiang, Lijun Wu, Xiao Men, Chang Qi, Guoqiang Chen, Haibo Zhang, Yi Wang, Mo Xian
Microbes on aging flue-cured tobaccos (ATFs) improve the aroma and other qualities desirable in products. Understanding the relevant organisms would picture microbial community diversity, metabolic potential, and their applications. However, limited efforts have been made on characterizing the microbial quality and functional profiling. Herein, we present our investigation of the bacterial diversity and predicted potential genetic capability of the bacteria from two AFTs using 16S rRNA gene sequences and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) software...
June 5, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29869678/implementing-electric-potential-difference-as-a-new-practical-parameter-for-rapid-and-specific-measurement-of-minimum-inhibitory-concentration-of-antibiotics
#18
Nasrin Mobasheri, Mehrdad Karimi, Javad Hamedi
New methods to determine antimicrobial susceptibility of bacterial pathogens especially the minimum inhibitory concentration (MIC) of antibiotics have great importance in pharmaceutical industry and treatment procedures. In the present study, the MIC of several antibiotics was determined against some pathogenic bacteria using macrodilution test. In order to accelerate and increase the efficiency of culture-based method to determine antimicrobial susceptibility, the possible relationship between the changes in some physico-chemical parameters including conductivity, electrical potential difference (EPD), pH and total number of test strains was investigated during the logarithmic phase of bacterial growth in presence of antibiotics...
June 5, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29869093/antimicrobial-and-cytotoxic-properties-of-bioactive-metabolites-produced-by-streptomyces-cavourensis-ybq59-isolated-from-cinnamomum-cassia-prels-in-yen-bai-province-of-vietnam
#19
Hanh-Nguyen Thi Vu, Dat Tien Nguyen, Huy Quang Nguyen, Ha Hoang Chu, Son Ky Chu, Minh Van Chau, Quyet-Tien Phi
The endophytic actinomycete strain YBQ59 was isolated from Cinnamomum cassia Prels in Yen Bai province (21°53'14″N; 104°35'9″E) of northern Vietnam. Based on analysis of morphological, physiological characteristics and 16S rRNA gene sequence (GenBank Acc. No. MF950891), the strain YBQ59 possessed high similarity to Streptomyces cavourensis subsp. cavourensis strain NRRL 2740, therefore assigned as S. cavourensis YBQ59. The ethyl acetate extract of the YBQ59 culture broth isolated eight pure secondary metabolites, identified as 1-monolinolein (1), bafilomycin D (2), nonactic acid (3), daidzein (4), 3'-hydroxydaidzein (5), 5,11-epoxy-10-cadinanol (6), prelactone B (7), and daucosterol (8)...
June 4, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29858620/complete-genome-sequence-of-actinosynnema-pretiosum-x47-an-industrial-strain-that-produces-the-antibiotic-ansamitocin-ap-3
#20
LETTER
Chuanqing Zhong, Gongli Zong, Shulan Qian, Meng Liu, Jiafang Fu, Peipei Zhang, Jun Li, Guangxiang Cao
Ansamitocins are extraordinarily potent antitumor agents. Ansamitocin P-3 (AP-3), which is produced by Actinosynnema pretiosum, has been developed as a cytotoxic drug for breast cancer. Despite its importance, AP-3 is of limited applicability because of the low production yield. A. pretiosum strain X47 was developed from A. pretiosum ATCC 31565 by mutation breeding and shows a relatively high AP-3 yield. Here, we analyzed the A. pretiosum X47 genome, which is ~8.13 Mb in length with 6693 coding sequences, 58 tRNA genes, and 15 rRNA genes...
June 1, 2018: Current Microbiology
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