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Current Microbiology

Fei Shang, Ting Xue, Man Wang, Xiaolin Chen, Li Yu, Ming Zhang
A Gram-negative, rod-shaped, salt-tolerant, non-pigmented, and non-spore-forming bacterium, designated strain W1(T) (type strain CICC 23797 = CGMCC1.14949), was isolated from sewage samples of a dairy farm in Bozhou, Anhui, China. Strain W1 was resistant to lincomycin, troleandomycin, rifamycin, and vancomycin. Sequence analysis of the 16S rDNA gene revealed that the strain showed sequence similarity of 98.2% with the closest related species Serratia quinivorans CP6a(T). The genomic DNA G+C content of the isolate was 52...
April 20, 2017: Current Microbiology
Zheng Gong, Min Wang, Qingwei Yang, Zhongshi Li, Jun Xia, Yu Gao, Yong Jiang, Xue Meng, Zhaoyang Liu, Ding Yang, Fangfei Zhang, Hongbing Shao, Duobing Wang
Phage PH357, a novel lytic Pseudoalteromonas lipolytica phage belonging to the Myoviridae family was isolated from the Yangtze River estuary. The microbiological characterization demonstrated that phage PH357 is stable from -20 to 60 °C and the optimal pH 7. The one-step growth curve showed a latent period of 20 min, a rise period of 20 min, and the average burst size was about 85 virions per cell. Complete genome of phage PH357 was determined. Genome of phage PH357 consisted of a linear, double-stranded 136,203 bp DNA molecule with 34...
April 20, 2017: Current Microbiology
Yilin Qu, Rongyan Li, Mingshan Jiang, Xiuhong Wang
Because of heavy use of antimicrobials, antimicrobial resistance in bacteria has become of great concern. The effect of some widely used food additives such as sucralose on bacteria in the gut and the environment has also drawn increasing attention. In this study, we investigated the interaction between antimicrobials and sucralose impacting antimicrobial resistance and mutation of Escherichia coli (E. coli). To examine antimicrobial resistance and mutation frequency, different subinhibitory concentrations of sucralose were added to cultures of E...
April 20, 2017: Current Microbiology
Xue Meng, Min Wang, Siyuan You, Duobing Wang, Yan Li, Zhaoyang Liu, Yu Gao, Lu Liu, Yaoyuan Zhang, Zhenghao Yan, Chunyan Liu, Yong Jiang, Hongbing Shao
A novel Siphoviridae family Phage BS5, which infects Pseudoalteromonas atlantica, was isolated from the surface waters of the Yellow Sea. Morphological study by transmission electron microscopy revealed that the novel phage belongs to Siphoviridae. The complete genome sequence of PBS5 contained a linear, double-strand 39949-bp DNA molecule with a G + C content of 40.6% and 65 putative open reading frames. Twelve conserved domains were detected by BLASTP in NCBI, and of these the functions of 5 were known...
April 20, 2017: Current Microbiology
Yu Gao, Qian Liu, Min Wang, Guihua Zhao, Yong Jiang, Gill Malin, Zheng Gong, Xue Meng, Zhaoyang Liu, Tongtong Lin, Yutong Li, Hongbing Shao
A novel marine Alteromonas gracilis siphovirus, phage PB15, was isolated from the surface water of the Yellow Sea in August 2015. It has a head diameter of 58 ± 5 nm head and a contractile tail approximately 105 ± 10 nm in length, and overall, the morphology suggests that PB15 belongs to the family Siphoviridae. PB15 phage is stable at over the temperature range 0-60 °C. The best MOI of these phage was 0.1, and infectivity decreased above 60 °C. The results suggest that phage is stable at pH value ranging between 3...
April 20, 2017: Current Microbiology
Su-Jin Park, Jae-Jin Lee, Seung-Yeol Lee, Dae Sung Lee, Myung Kyum Kim, Leonid N Ten, Hee-Young Jung
A Gram-negative, short rod-shaped, gliding motile, and pale pink-pigmented bacterial strain, designated 15J9-9(T), was isolated from the soil of Iho Tewoo Beach, Jeju Island, Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J9-9(T) belonged to the family Cytophagaceae and was related to Larkinella bovis M2T2B15(T) (96.5% similarity), Larkinella arboricola Z0532(T) (95.3% similarity), and Larkinella insperata LMG 22510(T) (95...
April 17, 2017: Current Microbiology
Yousri Abdelmutalab Abdelhafiz, Thamilvaani Manaharan, Saharuddin BinMohamad, Amir Feisal Merican
The draft genome here presents the sequence of Bacillus subtilis UMX-103. The bacterial strain was isolated from hydrocarbon-contaminated soil from Terengganu, Malaysia. The whole genome of the bacterium was sequenced using Illumina HiSeq 2000 sequencing platform. The genome was assembled using de novo approach. The genome size of UMX-103 is 4,234,627 bp with 4399 genes comprising 4301 protein-coding genes and 98 RNA genes. The analysis of assembled genes revealed the presence of 25 genes involved in biosurfactant production, where 14 of the genes are related to biosynthesis and 11 of the genes are in the regulation of biosurfactant productions...
April 17, 2017: Current Microbiology
Yongyi Ge, Fengming Yu, Yumei Tan, Xiaoling Zhang, Zuoyi Liu
Aspergillus cristatus undergoes sexual and asexual development under conditions of low and high osmotic pressure, respectively. In this study, the expression levels of 107 genes associated with sexual and asexual development were analysed under conditions of low and high osmotic pressure by RNA sequencing. The results showed that 37 genes were up-regulated and other genes were down-regulated under conditions of high osmotic pressure, with most of the up-regulated genes associated with asexual development and most down-regulated genes associated with sexual development...
April 17, 2017: Current Microbiology
Yi Jin, Guojuan Gan, Xiaoyun Yu, Dongdong Wu, Li Zhang, Na Yang, Jiadan Hu, Zhiheng Liu, Lixin Zhang, Huachang Hong, Xiaoqing Yan, Yan Liang, Linxian Ding, Yonglong Pan
Printing and dyeing wastewater with high content of organic matters, high colority, and poor biochemical performance is hard to be degraded. In this study, we isolated viable but non-culturable (VBNC) bacteria from printing and dyeing wastewater with the culture media contained resuscitation promoting factor (Rpf) protein secreted by Micrococcus luteus, counted the culturable cells number with the most probable number, sequenced 16S rRNA genes, and performed polymerase chain reaction-denaturing gradient gel electrophoresis...
April 17, 2017: Current Microbiology
Leonid N Ten, Jae-Jin Lee, Yeon-Hee Lee, Su-Jin Park, Seung-Yeol Lee, Sangkyu Park, Dae Sung Lee, In-Kyu Kang, Myung Kyum Kim, Hee-Young Jung
No abstract text is available yet for this article.
April 12, 2017: Current Microbiology
Kurt T Ash, Kristina M Drake, Whitney S Gibbs, Bert Ely
The genomes of the type B3 bacteriophages that infect Caulobacter crescentus are among the largest phage genomes thus far deposited into GenBank with sizes over 200 kb. In this study, we introduce six new bacteriophage genomes which were obtained from phage collected from various water systems in the southeastern United States and from tropical locations across the globe. A comparative analysis of the 12 available genomes revealed a "core genome" which accounts for roughly 1/3 of these bacteriophage genomes and is predominately localized to the head, tail, and lysis gene regions...
April 10, 2017: Current Microbiology
Adi Setyo Purnomo, Refdinal Nawfa, Fahimah Martak, Kuniyoshi Shimizu, Ichiro Kamei
No abstract text is available yet for this article.
April 10, 2017: Current Microbiology
Weimin Chen, Hongmei Chai, Weixian Yang, Xiaolei Zhang, Yuhui Chen, YongChang Zhao
Agrocybe salicacola is a delicious and cultivable mushroom. It is important to understand this species' inherent characteristics, especially to elucidate the constitution and segregation of mating genes. In this study, two compatible B mating loci in strain YAASM0711 of A. salicacola were cloned from the monokaryons, and sequence and phylogeny analyses showed two conserved genes encoding pheromone receptors maybe lost mating activity, which determined by comparing with those of other mushrooms. In the conserved regions of mating loci, partial insertion/deletion fragments made non-coding regions posses polymorphisms, and monokaryotic strains of different mating types were distinguished from each other according to the amplification profile of variable regions, which suggested mating loci were integrally assigned to offspring strains during mitosis in A...
April 10, 2017: Current Microbiology
Thamir H Al-Kahem Al-Balawi, Adam L Wood, Alexis Solis, Ted Cooper, Ravi D Barabote
We describe the isolation and characteristics of a novel thermophilic bacterium from soil. The organism is a member of the Anoxybacillus genus based on phylogenetic analysis of the 16S rRNA gene. The 16S rRNA of the organism shares >99% sequence identity with those of two species, Anoxybacillus rupiensis and A. geothermalis. We named this isolate as Anoxybacillus sp. strain UARK-01. UARK-01 grows optimally in the presence of oxygen at 55 °C and pH 8. It grew excellently in the presence of lignin as the sole carbon source...
April 8, 2017: Current Microbiology
Xia Zhang, Chaowang Zhu, Juan Yin, Yang Sui, Yingchao Wang, Guanghua Zhai
During the infectious procedure of Salmonella enterica serovar Typhi (S. Typhi), Salmonella would suffer from some severe environmental stresses, such as gastric acid stress, enteric hyperosmotic stress, bile acids stress, and oxidative stress. S. Typhi must regulate the expression of numerous genes through the complex regulatory network to adapt strict stresses. RpoS, which encodes sigma factor σ(s), was reported to be a very important regulator in the maximal survival of enteric pathogens including S. enterica in the stress conditions...
April 6, 2017: Current Microbiology
D M Hunupolagama, N V Chandrasekharan, W S S Wijesundera, H S Kathriarachchi, T H P S Fernando, R L C Wijesundera
Colletotrichum is an important fungal genus with great diversity, which causes anthracnose of a variety of crop plants including rubber trees. Colletotrichum acutatum and Colletotrichum gloeosporioides have been identified as the major causative agents of Colletotrichum leaf disease of rubber trees in Sri Lanka based on morphology, pathogenicity, and the analysis of internally transcribed spacer sequences of the nuclear ribosomal DNA. This study has been conducted to investigate the members of the C. acutatum species complex causing rubber leaf disease using a morphological and multi gene approach...
April 5, 2017: Current Microbiology
Qisong Hu, Jiachao Zhang, Chuanbiao Xu, Congfa Li, Sixin Liu
White pepper (Piper nigrum L.), a well-known spice, is the main pepper processing product in Hainan province, China. The solid-state method of fermentation can peel pepper in a highly efficient manner and yield high-quality white pepper. In the present study, we used next-generation sequencing to reveal the dynamic changes in the microbiota during pepper peeling by solid-state fermentation. The results suggested that the inoculated Aspergillus niger was dominant throughout the fermentation stage, with its strains constituting more than 95% of the fungi present; thus, the fungal community structure was relatively stable...
April 4, 2017: Current Microbiology
Qing Pan, Xiaoqun Zeng, Daodong Pan, Liuyang Peng, Zhen Wu, Yangying Sun, Yan Wei
The proliferation mechanism of Lactobacillus plantarum RB1 promoted by stachyose was investigated in this work. The hydrolysis of stachyose, the glycometabolism, and the cytoactivity of L. plantarum RB1 were detected after proliferation. The specific activity of α-galactosidase of L. plantarum RB1 in the stachyose group was significantly higher than the control group (without stachyose), which indicated that the stachyose induced L. plantarum RB1 and produced more α-galactosidase to hydrolyze stachyose. The glycometabolism which includes glycolysis and tricarboxylic acid (TCA) cycle was significantly enhanced in the stachyose group compared with the control group...
April 4, 2017: Current Microbiology
Aslıhan Örs Gevrekci
Polyamines are well-conserved, multifunctional polycations that contribute to a number of processes in the cells such as cell cycle, apoptosis, stress response, and gene expression. Therefore, polyamine levels should be kept under strict regulation by specific polyamine transporters and polyamine synthases. In this study, the aim is to experimentally characterize a predicted spermidine synthase gene srm1, which was identified upon sequence similarity, in fission yeast Schizosaccharomyces pombe. In an attempt to understand the role of this gene in cell cycle and stress response, deletion mutant of srm1 was generated and analyzed in terms of cell cycle regulation and environmental stress response...
March 27, 2017: Current Microbiology
Yuwei Ren, Sisi Li, Zhixin Wu, Chengchong Zhou, Ding Zhang, Xiaoxuan Chen
The aim of this study was to explore the influence of Bacillus subtilis CH9 on Aeromonas hydrophila SC2005. The transcription level of virulence genes of A. hydrophila SC2005 and its hemolysin activity as well as its cytotoxicity were analyzed when B. subtilis CH9 and A. hydrophila SC2005 were co-cultured. The results indicated that the transcription levels of four virulence genes of A. hydrophila, including aer, ahyB, hcp, and emp, decreased when A. hydrophila was cultured with B. subtilis CH9. Furthermore, the extracellular products of A...
March 24, 2017: Current Microbiology
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