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Animal Reproduction Science

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https://www.readbyqxmd.com/read/29789185/variation-in-post-thaw-sperm-quality-of-white-tailed-deer-bucks-odocoileus-virginianus-during-rut
#1
Jamie L Stewart, Clifford F Shipley, Robyn E Ellerbrock, Fabio S Lima, Igor F Canisso
White-tailed deer farming is a growing industry in the United States, with breeding operations contributing significantly to the industry's economic impact. Artificial insemination with frozen semen allows for selection and dissemination of valuable genetics, yet surprisingly little is known regarding the best time throughout rut to perform semen cryopreservation. The objective of this study was to compare semen quality following cryopreservation of white-tailed deer bucks collected early in the breeding season (September, n = 6), at peak rut (December, n = 8), and late season (March, n = 7)...
May 19, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29778272/development-of-the-waveless-bovine-model
#2
F Jimenez-Krassel, J L H Ireland, C Kronemeyer, A Wilson-Alvarado, J J Ireland
Development of a bovine model without ovarian follicular waves (waveless) and transient increases in gonadotropin secretion during estrous cycles may lead to new methods to more consistently regulate ovulatory follicle growth thereby improving efficiency of embryo transfer. We hypothesized that the GnRH antagonist acyline would inhibit gonadotropin secretion thereby blocking follicular waves, ovarian function and ovulation during estrous cycles of cattle. To test this hypothesis, beef heifers (n = 5 per group) were treated twice daily with vehicle (control) or 25 or 50 μg/kg acyline beginning 12 h after GnRH-induced ovulation and ending 21 days later...
May 15, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29778271/evaluation-of-environmental-effects-on-reproductive-characteristics-of-mangalarga-marchador-mares-in-a-commercial-embryo-transfer-program
#3
Miguel Alejandro Silva Rua, Celia Raquel Quirino, Ana Cláudia Cerqueira Rodrigues, Mirela Mendes Christo, Marcus Antônio Pessanha Barreto
The objective of this study was to evaluate the environmental effects on embryo recovery rate and pregnancy rate of Mangalarga Marchador mares. The reproductive characteristics of donor and recipient mares were evaluated during five years in Brazilian tropical environment. The mares were used throughout the year and seasons were classified as: October to April (breeding season - BS); May (autumn transition out of the breeding season - ATBS); June to August (non-breeding season - nBS); and September (vernal transition into the breeding season - VTBS)...
May 15, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29776697/cinnamtannin-b-1-a-novel-antioxidant-for-sperm-in-red-deer
#4
F Sánchez-Rubio, M R Fernández-Santos, L Castro-Vázquez, O García-Álvarez, A Maroto-Morales, A J Soler, F Martínez-Pastor, J J Garde
Cinnamtannin B-1 (CNB-1) is a naturally occurring trimeric A-type proanthocyanidin contained in several plants such as cinnamon (Cinnamomum zeylanicum). It is considered to be a potent antioxidant. The protective effect of CNB-1 against oxidative stress was assessed in red deer epididymal sperm incubated at 37 °C. Cryopreserved sperm from six stags were thawed, pooled and extended to 400 × 106 sperm/ml in BGM (bovine gamete medium). After being aliquoted, the samples were supplemented with different concentrations of CNB-1 (0, 0...
May 15, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29776696/single-layer-centrifugation-improves-the-quality-of-frozen-thawed-sperm-of-giant-panda-ailuropoda-melanoleuca
#5
Z G Cai, J H An, Y L Liu, S M Yie, Y Zhang, F P Li, J S Chen, X Wang, J M Morrell, R Hou
The reproductive capacity of captive giant pandas is poor and sperm cryopreservation is necessary for the reproduction and conservation of this species. Cryopreservation, however, leads to a significant decrease in sperm quality, including sperm motility, acrosome integrity and DNA integrity. In the present study, a method was developed based on colloid single layer centrifugation that could significantly improve frozen-thawed sperm quality. Two colloids were compared for post-thaw giant panda sperm preparation; the sperm samples had greater total motility (Colloid 1: 44...
May 15, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29773470/is-boar-sperm-freezability-more-intrinsically-linked-to-spermatozoa-than-to-the-surrounding-seminal-plasma
#6
Junwei Li, Jordi Roca, Cristina Pérez-Patiño, Isabel Barranco, Emilio A Martinez, Heriberto Rodriguez-Martinez, Inmaculada Parrilla
This study aimed to elucidate the effect of seminal plasma (SP) from post-SRF on boar sperm freezability and, in addition, to determine the relevance of sperm itself to sustain cryopreservation, regardless of the SP surrounding them. Twelve ejaculates from three boars were manually collected in fractions/portions, P1: the first 10 mL of the SRF, P2: the rest of the SRF and the post-SRF. Immediately, samples were centrifuged to separate spermatozoa from the surrounding SP. Spermatozoa from P1 and P2 were then incubated with its own SP or that from post-SRF, diluted in BTS (1:1, v/v) at 17 °C overnight before being frozen in 0...
May 14, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29764712/the-tail-in-tropical-hair-ewes-ovis-aries-that-are-in-estrus-is-used-as-a-proceptive-signal-and-favors-ram-copulation
#7
Ángel Fierros-García, Rodolfo Ungerfeld, Virginio Aguirre, Agustín Orihuela
Two experiments were performed to determine if the tail has a role: 1) in proceptive behavior, and 2) in facilitating copulation in hair sheep. The movements and position of the tail of estrous and non-estrous ewes in response to male courtship were compared in the first study. In the second study, the courtship of rams and mating behavior directed towards tailed or tail-docked, in estrus or diestrus ewes, were compared. Both experiments were conducted with restrained ewes during 3 min assessment periods...
May 12, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29759910/genetic-parameters-predictions-and-rankings-for-semen-production-traits-in-a-thailand-multi-breed-dairy-population-using-genomic-polygenic-and-polygenic-models
#8
Mattaneeya Sarakul, Mauricio A Elzo, Skorn Koonawootrittriron, Thanathip Suwanasopee, Danai Jattawa
The objectives were to compare estimates of variance components, genetic parameters, prediction accuracies, and rankings of bulls for semen volume (VOL), number of sperm (NS), and motility (MOT) using genomic-polygenic (GPRM) and polygenic repeatability models (PRM). The dataset comprised 13,535 VOL, 12,773 NS, and 12,660 MOT from 131 bulls collected from 2001 to 2017 in the Semen Production and Dairy Genetic Evaluation Center of the Dairy Farming Promotion Organization of Thailand. Genotypic data encompassed 76,519 actual and imputed SNP from 72 animals...
May 12, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29753535/ovopel-%C3%A2-and-carp-pituitary-extract-for-induction-of-reproduction-in-colossoma-macropomum-females
#9
Francielle Novaes Souza, Edenilce de Fatima Ferreira Martins, Ruy Alberto Caetano Corrêa Filho, Janessa Sampaio de Abreu, Luana Barbosa Pires, Danilo Pedro Streit, Nelson Mauricio Lopera-Barrero, Jayme Aparecido Povh
The aim of this study was to evaluate the efficiency of the hormonal inducers Ovopel® and carp pituitary extract (CPE) for induction of reproduction in Colossoma macropomum females. The treatments were CPE at the dose of 5.5 mg/kg divided into two applications (10%; and 90% after 12 h) and Ovopel® at doses of 0.2 and 0.4 pellet/kg body weight in a single application. Eight replicates were used in each of the three treatments, totaling 24 experimental units. The females spawned when treated with the 0...
May 4, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29735243/effect-of-semen-processing-methods-on-lumpy-skin-disease-virus-status-in-cryopreserved-bull-semen
#10
C Henry Annandale, Mario P Smuts, Karen Ebersohn, Lizette du Plessis, Estelle H Venter, Tom A E Stout
Lumpy skin disease is an economically important disease of cattle, caused by the lumpy skin disease virus (LSDV; Capripoxvirus). It has a variable clinical appearance but, in severely affected animals, is associated with extensive skin damage, pneumonia and death. The LSDV can be found in the semen of infected bulls for prolonged periods of time, from where it can be transmitted by mating or artificial insemination and cause clinical disease in heifers and cows. In this study, an ejaculate was collected from a LSDV seronegative bull and confirmed free from LSDV DNA by PCR...
April 30, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29728276/ameliorative-effect-of-chitosan-conjugated-17%C3%AE-methyltestosterone-on-testicular-development-in-clarias-batrachus
#11
Ratnadeep Saha, Irfan Ahmed Bhat, Ravi Charan, Suresh Babu Padinhate Purayil, Gopal Krishna, Annam Pavan Kumar, Rupam Sharma
Chitosan nanoparticles conjugated with 17α-methyltestosterone (CS + MT) were used for studying their effect on the testicular development of Clarias batrachus during different reproductive phases. The size of chitosan nanoparticles was 127.2 nm and the nano-conjugated 17α methyltestosterone (17α-MT) was 196.1 nm (20 mg/100 ml of chitosan). Single injections of CS + MT at different doses such as 0.01, 0.1 and 0.5 μg/g body weight were administered to adults during the pre-spawning, spawning and post-spawning phase...
April 26, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29728275/insertion-of-275-bp-sine-into-first-intron-of-pdia4-gene-is-associated-with-litter-size-in-xiang-pigs
#12
Chang Liu, Xueqin Ran, Xi Niu, Sheng Li, Jiafu Wang, Qin Zhang
The aim of the study was to investigate the SINE polymorphism in Xiang, Kele, Qianbei black, Jiangkouluobo, Large White, and Duroc pig breeds. The PCR based detection method was conducted to identify the short interspersed nuclear element (SINE) polymorphism in the PDIA4 gene. There were greater frequencies of the SINE-/- genotypes in Xiang pigs (55.9%) as compared with other pig breed groups. There was an association between this 275 bp SINE polymorphism and litter size (P = 0.003). The homozygous SINE+/+ genotype of the PDIA4 gene had a 1...
April 26, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29731243/sperm-quality-assays-how-good-are-they-the-horse-perspective
#13
REVIEW
Charles C Love
Sperm quality assays have increased in number in the last 10 years. Most of these assays are flow cytometry based in application and are modified from assays that have been developed to measure somatic cell function. The goal of any sperm quality assay should be to advance the clinicians/researchers understanding of sperm cell function and the relationship to fertility. While these assays appear to measure somatic cell-like functions in sperm there tends to be little understanding how the results of these assays relate to fertility...
April 22, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29699920/effect-of-cholesterol-loaded-cyclodextrin-on-semen-cryopreservation-of-aksaray-malakli-shepherd-dogs-of-different-ages
#14
Muhammed Enes Inanc, Koray Tekin, Kemal Tuna Olgac, Burak Yilmaz, Beste Cil, Umut Tasdemir, Purhan Barbaros Tuncer, Serhat Buyukleblebici, Emre Durmaz, Ongun Uysal
The objective of the study was to determine the effect of cholesterol-loaded cyclodextrin (CLC) on the quality parameters of semen from Aksaray Malakli Shepherd dogs of different age groups. Forty-eight male dogs were divided into 3 groupings according to their ages (young age (Y): ≤3 years, n: 20; middle age (M): 4-6 years, n: 20; old age (O): ≥7 years; n: 8). The sperm-rich portion of the ejaculate from each dog was divided into four aliquots and extended with either tris as a control (C) or tris loaded with 0...
April 21, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29699918/cyclodextrins-or-cholesterol-loaded-cyclodextrins-a-better-choice-for-improved-cryosurvival-of-chicken-spermatozoa
#15
Agnieszka Partyka, Maciej Strojecki, Wojciech Niżański
This study was designed to test if treating chicken sperm with i) the cyclodextrins 2-hydroxypropyl-β-cyclodextrin (HBCD) and methyl-β-cyclodextrin (MBCD) alone improve fresh, liquid-stored and cryopreserved semen quality, or ii) cholesterol-loaded cyclodextrins (CLCs): 2-hydroxypropyl-β-cyclodextrin loaded with cholesterol (HCLC) and methyl-β-cyclodextrin loaded with cholesterol (MCLC) enhance chicken semen quality for application to assisted reproductive technologies. Three consecutive experiments were performed with different concentrations of additives: Exp...
April 21, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29753534/phenotypic-varieties-of-sperm-pathology-genetic-abnormalities-or-environmental-influences-can-result-in-different-patterns-of-abnormal-spermatozoa
#16
REVIEW
Hector E Chemes
The present paper reviews in detail ultrastructural and molecular studies addressed to characterize different phenotypes of sperm pathology in sterile men. In each case ultrastructural, immunocytochemical, molecular and genetic information is provided to differentiate two main kinds of sperm pathologies: systematic phenotypes with known or suspected genetic origin and non-systematic ones, usually secondary to different pathologies of the male reproductive system. Special attention is paid to detailed ultrastructural features profusely illustrated with electron micrographs...
April 20, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29716779/effect-of-maternal-metabolism-on-fetal-supply-glucose-non-esterified-fatty-acids-and-beta-hydroxybutyrate-concentrations-in-canine-maternal-serum-and-fetal-fluids-at-term-pregnancy
#17
Orsolya Balogh, Rupert Bruckmaier, Stefanie Keller, Iris Margaret Reichler
The progressive adaptations in carbohydrate and lipid metabolism during canine pregnancy are reflected in the concentrations of glucose, non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB). The levels of these metabolites in the bitch likely affect fetal concentrations and the composition of amniotic and allantoic fluids (AMF and ALF, respectively). We studied 31 canine parturitions (Cesarean sections) and found that glucose, NEFA and BHB concentrations were significantly higher in maternal serum than in AMF or ALF...
April 20, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29699919/effect-of-cooling-rate-on-sperm-quality-of-cryopreserved-andalusian-donkey-spermatozoa
#18
S Demyda-Peyrás, M Bottrel, D Acha, I Ortiz, M Hidalgo, J J Carrasco, V Gómez-Arrones, J Gósalvez, J Dorado
The aim of this study was to evaluate the effect of different cooling rates on post-thaw quality of cryopreserved donkey spermatozoa. Eighteen ejaculates from six adult Andalusian donkeys (three ejaculates per donkey) were collected using an artificial vagina. Pooled semen samples (two ejaculates per pool) were divided into three aliquots, and frozen in Gent freezing extender using three different cryopreservation protocols (P): P1 (conventional slow freezing, as control): semen pre-cooled in an Equitainer for 2 h and frozen in liquid nitrogen (LN2 ) vapour; P2 (controlled pre-freeze cooling rate): semen pre-cooled at a controlled rate for 73 min and frozen in LN2 vapour; and P3 (rapid freezing) semen frozen immediately in LN2 vapour...
April 20, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29705131/effect-of-carp-pituitary-homogenate-cph-and-sgnrha-ovaprim-on-northern-pike-esox-lucius-spermiation-stimulation-and-its-effect-on-quantity-and-quality-of-sperm
#19
Beata Irena Cejko, Sławomir Krejszeff, Daniel Żarski, Sylwia Judycka, Katarzyna Targońska, Dariusz Kucharczyk
The effect of carp pituitary homogenate (CPH, n = 7) at a dose of 2.0 mg kg-1 and (D-Arg6 , Pro9 NET)-sGnRH + domperidone (Ovaprim, n = 7) at a dose of 0.5 ml kg-1 in northern pike (Esox lucius) sperm maturation under controlled conditions was examined. On the control group, 0.9% NaCl at a dose of 1.0 ml kg-1 (n = 7) was used. Sperm was collected 48 h following injection. Sperm quantity (total sperm volume, total sperm production and sperm concentration), sperm motility using the Computer-assisted sperm analysis (CASA) system and sperm and seminal plasma quality (sperm pH, seminal plasma osmotic pressure and seminal plasma pH) were determined for each male separately in each group...
April 19, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29703418/relationship-between-sperm-motility-characteristics-and-atp-concentrations-and-association-with-fertility-in-two-different-pig-breeds
#20
Nina Hårdnes Tremoen, Ann Helen Gaustad, Ina Andersen-Ranberg, Maren van Son, Teklu Tewoldebrhan Zeremichael, Kirsten Frydenlund, Eli Grindflek, Dag Inge Våge, Frøydis Deinboll Myromslien
Boar fertility has a major impact on overall pig reproductive efficiency. Using accurate and objective in vitro sperm variables for predicting in vivo fertility from a single ejaculate, however, is challenging. Motility is the most widely used indicator of sperm quality, and a computer assisted sperm analysis (CASA) system is now available for objective assessment of sperm motility characteristics. In this study sperm motility characteristics and semen ATP concentrations were investigated and the effect of both were evaluated on total number of piglets born (TNB) when Norwegian Landrace (NL) and Norwegian Duroc (ND) boar semen was used for AI...
April 19, 2018: Animal Reproduction Science
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