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Animal Reproduction Science

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https://www.readbyqxmd.com/read/28302333/analysis-of-common-carp-cyprinus-carpio-sperm-motility-and-lipid-composition-using-different-in-vitro-temperatures
#1
Hadiseh Dadras, Sabine Sampels, Amin Golpour, Viktoriya Dzyuba, Jacky Cosson, Borys Dzyuba
In fish, sperm quality is frequently associated with sperm motility variables. The response of sperm motility to different temperatures varies among species and plasma membrane lipid composition may contribute to variations in findings in previous research. In the present study, sperm motility and lipid composition were analysed between motile or immotile carp Cyprinus carpio sperm at different in vitro temperatures (4, 14 and 24°C). The duration of the period over which sperm motility is sustained was longer at 4°C compared with 14 and 24°C; while sperm velocity was greatest at 24°C...
March 6, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28285882/effect-of-altering-the-intervals-between-consecutive-superovulatory-doses-of-porcine-follicle-stimulating-hormone-on-ovarian-responses-and-embryo-yields-in-anestrous-ewes
#2
P M Bartlewski, M Murawski, T Schwarz, M E F Oliveira
The effect of varying intervals between successive gonadotropin injections on the superovulatory outcomes in anestrous Rideau Arcott ewes superstimulated for ovarian follicular development with multiple doses of porcine FSH (pFSH) was evaluated in a single study. Twenty-five animals received six (1×2.5ml and 5×1.25ml) injections of Folltropin(®)-V given at 0800 and 1600h or at 0800 and 2000h in Group 1 (n=9) or Group 2 (n=16), respectively. An i.m. injection of 500 IU of equine chorionic gonadotropin (eCG; Folligon(®)) was given concurrently with the first pFSH dose...
March 6, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28262463/spermatozoa-with-high-mitochondrial-membrane-potential-and-low-tyrosine-phosphorylation-preferentially-bind-to-oviduct-explants-in-the-water-buffalo-bubalus-bubalis
#3
Kaustubh Kishor Saraf, Arumugam Kumaresan, Shivani Chhillar, Samiksha Nayak, Sreela Lathika, Tirtha Kumar Datta, Subhash Chand Gahlot, Prabha Karan, Kiran Verma, Tushar Kumar Mohanty
Although it is understood that spermatozoa are subjected to selection processes to form a functional sperm reservoir in the oviduct, the mechanism remains obscure. With the aim to understand the sperm selection process in the oviduct, in the present in vitro study, we analyzed mitochondrial membrane potential and tyrosine phosphorylation status in oviduct-explants bound and unbound spermatozoa. Frozen semen from Murrah buffalo bulls (n=10) used under progeny testing programme were utilized for the study. Oviduct explants were prepared by overnight culture of epithelial cells in TCM- 199 and washed spermatozoa were added to the oviduct explants and incubated for 4h...
February 28, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28268040/long-term-liquid-storage-and-reproductive-evaluation-of-an-innovative-boar-semen-extender-formula12-%C3%A2-containing-a-non-reducing-disaccharide-and-an-enzymatic-agent
#4
Carla Bresciani, Annalisa Bianchera, Ruggero Bettini, Annamaria Buschini, Laura Marchi, Clotilde Silvia Cabassi, Alberto Sabbioni, Federico Righi, Claudio Mazzoni, Enrico Parmigiani
There are no reports of saccharolytic enzymes being used in the preparation of formulations for animal semen extenders. In the present study, the use of an innovative semen extender (Formula12(®)) in the long-term liquid storage of boar semen at 17°C was evaluated. The formulation included use of a disaccharide (sucrose) as the energy source precursor coupled to an enzymatic agent (invertase). The innovative extender was evaluated and compared in vitro to a commercial extender (Vitasem LD(®)) for the following variables: Total Motility (TM), Forward Progressive Motility (FPM), sperm morphology, membrane integrity, acrosome integrity, and chromatin instability...
February 24, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28249701/effect-of-season-on-scrotal-circumference-semen-characteristics-seminal-plasma-composition-and-spermatozoa-motility-during-liquid-storage-in-inra180-rams
#5
Anass Benmoula, Abdelmoughit Badi, Moussa El Fadili, Kaoutar El Khalil, Larbi Allai, Abderaouf El Hilali, Bouchra El Amiri
The present study was undertaken to assess the effect of seasons on scrotal circumference, semen characteristics, seminal plasma composition, and sperm motility during liquid storage of INRA180 rams. The semen was collected from five mature INRA180 rams (2-3 years of age) during one year (from April 2014 to March 2015). Scrotal circumferences, semen characteristics, some biochemical parameters of seminal plasma were evaluated. Immediately after collection and evaluation, the semen was pooled and extended in skim milk (SM) at 15°C to reach 0...
February 22, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28262464/assessment-of-cathepsin-mrna-expression-and-enzymatic-activity-during-early-embryonic-development-in-the-yellowtail-kingfish-seriola-lalandi
#6
Jaime Palomino, Giannina Herrera, Jorge Torres-Fuentes, Phillip Dettleff, Alok Patel, Víctor Martínez
In pelagic species such as Seriola lalandi, survival of both the eggs and embryos depends on yolk processing during oocyte maturation and embryo development. The main enzymes involved in these processes are the cathepsins, which are essential for the hydration process, acquiring buoyancy and nutrition of the embryo before hatching. This study aimed to investigate the mRNA expression profiles of cathepsins B, D and L (catb, catd and catl) and the activity of these enzymes during early development in S. lalandi...
February 21, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28258785/establishment-of-critical-timing-of-progesterone-supplementation-on-corpus-luteum-and-embryo-development-in-beef-heifers
#7
M H Parr, S Scully, P Lonergan, A C O Evans, M A Crowe, M G Diskin
Optimal concentrations of progesterone (P4) during early pregnancy are a major determinant of embryo survival in cattle. This study examined the effects of P4 supplementation, following a period of induced low P4, on corpus luteum (CL) development, circulating P4 concentrations and embryo development. A total of 107 beef heifers from one herd were used in the study. Following AI (Day 0) at a synchronized oestrus, heifers were randomly assigned to 1 of 5 treatment groups: (1) no subsequent treatment (control; n=15); (2) administration of a synthetic prostaglandin F2α analogue (PG) on Days 3, 3...
February 17, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28238531/fibroblast-growth-factor-2-fgf2-and-vascular-endothelial-growth-factor-a-vegfa-synergistically-promote-steroidogenesis-and-survival-of-cultured-buffalo-granulosa-cells
#8
S R Mishra, Jaya Bharati, G Rajesh, V S Chauhan, G Taru Sharma, S Bag, V P Maurya, G Singh, M Sarkar
The present study investigated the combined effect of fibroblast growth factor 2 (FGF2) and vascular endothelial growth factor A (VEGF-A) on estradiol (E2) secretion and relative abundance of mRNA for aromatase enzyme (CYP19A1), proliferating cell nuclear antigen (PCNA) and BCL-2 associated X protein (BAX) in cultured buffalo granulosa cells (GCs). Follicles were isolated and classified into four groups based on size and E2 concentration in follicular fluid (FF): Small, 4-6mm diameter, E2<0.5ng/ml; Medium, 7-9mm, E2=0...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28238530/a-membrane-associated-adenylate-cyclase-modulates-lactate-dehydrogenase-and-creatine-kinase-activities-required-for-bull-sperm-capacitation-induced-by-hyaluronic-acid
#9
Silvina Fernández, Mariana Córdoba
Hyaluronic acid, as well as heparin, is a glycosaminoglycan present in the female genital tract of cattle. The aim of this study was to evaluate oxidative metabolism and intracellular signals mediated by a membrane-associated adenylate cyclase (mAC), in sperm capacitation with hyaluronic acid and heparin, in cryopreserved bull sperm. The mAC inhibitor, 2',5'-dideoxyadenosine, was used in the present study. Lactate dehydrogenase (LDH) and creatine kinase (CK) activities and lactate concentration were determined spectrophotometrically in the incubation medium...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28238445/pentoxifylline-effects-on-capacitation-and-fertility-of-stallion-epididymal-sperm
#10
P N Guasti, G A Monteiro, R R D Maziero, M T Carmo, J A Dell'Aqua, A M Crespilho, E A Rifai, F O Papa
The aims of this study were to determinate whether pentoxifylline (PTX) increases the motion parameters of fresh and frozen-thawed equine epididymal spermatozoa, to evaluate the tyrosine phosphorylation of frozen-thawed epididymal sperm in the presence of PTX and to determine whether the PTX-treatment of stallion epididymal sperm prior to freezing improves the fertility response of mares to a reduced number of spermatozoa per insemination dose. Fifty epididymis were flushed with a skim milk based extender with or without PTX...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28233584/pdpn-gene-promotes-the-proliferation-of-immature-bovine-sertoli-cells-in-vitro
#11
Yi Gao, Lihong Qin, Yuwei Yang, Xue Dong, Zijiao Zhao, Guoliang Zhang, Zhihui Zhao
Podoplanin (PDPN) is a transmembrane receptor which is involved in various physiological and pathological processes, such as cell motility, invasion, tumor metastasis and blood vessels formation. Although there are reports on the involvement of PDPN in Sertoli cells in human and mice, the role of PDPN on the development of bovine Sertoli cells has not been reported. In the present study, Sertoli cells were isolated from 1-day-old bovine testes by two steps enzyme digestion method. Feulgen staining of satellite karyosomes and inhibin immunofluorescence staining suggested that the isolated immature Sertoli cells were very pure...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28228338/extra-and-intracellular-calcium-signaling-pathway-s-differentially-regulate-histamine-induced-myometrial-contractions-during-early-and-mid-pregnancy-stages-in-buffaloes-bubalus-bubalis
#12
Abhishek Sharma, Udayraj P Nakade, Soumen Choudhury, Rajkumar Singh Yadav, Satish Kumar Garg
This study examines the differential role of calcium signaling pathway(s) in histamine-induced uterotonic action during early and mid-pregnancy stages in buffaloes. Compared to mid pregnancy, tonic contraction, amplitude and mean-integral tension were significantly increased by histamine to produce myometrial contraction during early pregnancy with small effects on phasic contraction and frequency. Although uterotonic action of histamine during both stages of pregnancy is sensitive to nifedipine (a L-type Ca(2+) channels blocker) and NNC55-0396 (T-type Ca(2+) channels blocker), the role of extracellular calcium seems to be more significant during mid-pregnancy as in this stage histamine produced only 9...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28215454/sperm-quality-and-oxidative-status-as-affected-by-homogenization-of-liquid-stored-boar-semen-diluted-in-short-and-long-term-extenders
#13
Mariana B Menegat, Ana Paula G Mellagi, Rafael C Bortolin, Tila A Menezes, Amanda R Vargas, Mari Lourdes Bernardi, Ivo Wentz, Daniel P Gelain, José Cláudio F Moreira, Fernando P Bortolozzo
Homogenization of diluted boar semen during storage has for a long time been regarded as beneficial. Recent studies indicated an adverse effect of homogenization on sperm quality for yet unknown reasons. This study aimed to verify the effect of homogenization on sperm parameters and to elucidate the impact of oxidative stress. Twenty-one normospermic ejaculates (21 boars) were diluted with Androstar(®) Plus (AND) and Beltsville Thawing Solution (BTS). Semen doses were submitted to no-homogenization (NoHom) or twice-a-day manual homogenization (2xHom) during storage at 17°C for 168h...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28215453/beef-heifers-with-diminished-numbers-of-antral-follicles-have-decreased-uterine-protein-concentrations
#14
Anthony K McNeel, Émerson M Soares, Allyson L Patterson, Jeffrey L Vallet, Elane C Wright, Erin L Larimore, Olivia L Amundson, Jeremy R Miles, Chadwick C Chase, Clay A Lents, Jennifer R Wood, Andrea S Cupp, George A Perry, Robert A Cushman
Previous research demonstrated a favorable relationship between the number of follicles detectable in the bovine ovary by ultrasonography and fertility, and bovine females with diminished numbers of antral follicles had smaller reproductive tracts. Therefore, we hypothesized that uterine function would be compromised in beef heifers with diminished numbers of antral follilcles. Angus heifers (n=480) were submitted for ultrasonographic evaluation of antral follicle number at 325 and 355d of age. After the second ultrasonographic examination, 40 pubertal heifers with the greatest average number of antral follicles (30...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28214066/identification-of-g-170g-a-and-g-332g-a-mutations-in-exon-3-of-leptin-gene-bcnl-and-cail-and-their-association-with-semen-quality-and-testicular-dimensions-in-sanjabi-rams
#15
Roya Bakhtiar, Alireza Abdolmohammadi, Hadi Hajarian, Zahra Nikousefat, Davood Kalantar-Neyestanaki
The purpose of this study was to investigate leptin gene polymorphisms and their relationships with the characteristics of sperm quality and testicular dimensions. Semen samples were collected from 96 Sanjabi rams during autumn and spring seasons over two years. Simultaneously, the dimensions of length, width and scrotal circumference were measured. Blood samples were taken from the jugular vein to extract DNA. PCR was performed to amplify a 463bp fragment including exon 3 of leptin gene. PCR products were digested by Bcnl and Cail restriction enzymes to identify 170G>A and 332G>A mutations in exon 3, respectively...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28190662/prospective-ultrasonographic-and-endocrine-predictors-of-spermatogenic-onset-in-ram-lambs
#16
Pawel M Bartlewski, Jennifer L Giffin, Olutobi A Oluwole, Ann C Hahnel
The objective of this study was to examine testicular ultrasonographic characteristics and endocrine profiles in prepubescent ram lambs for correlations with the age at first detection of elongated spermatids (ESt age). Bi-weekly ultrasound examinations and weekly testicular biopsies began at 10 weeks of age or at the time that testicular volume reached 15cm(3), and continued until 1-2 weeks after Est's were first detected by histological examination of testicular biopsies in twenty-two spring-born Rideau Arcott×Polled Dorset lambs...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28189350/the-cryoprotective-effect-of-iodixanol-in-buffalo-semen-cryopreservation
#17
Dheer Singh Swami, Pradeep Kumar, R K Malik, Monika Saini, Dharmendra Kumar, M H Jan
This is the first report to examine the effect of iodixanol (OptiPrep(TM)) on cryosurvival of buffalo spermatozoa. A total of thirty ejaculates (five ejaculates from each bull) from six buffalo bulls were used for this experiment. Each ejaculate was divided into four aliquots and diluted in freezing extender supplemented with different concentrations of OptiPrep(TM) (0, 1.25, 2.5 and 5%) and then cryopreserved. The semen quality variables were evaluated before and after freezing of the semen. There were no effects of OptiPrep(TM) (P>0...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28189349/the-reproductive-performance-of-female-goats-treated-with-melatonin-is-not-improved-after-introduction-to-bucks-displaying-springtime-sexual-activity-if-these-does-are-experiencing-decreasing-body-weight-condition-score
#18
L A Zarazaga, M C Gatica, L Gallego-Calvo, J L Guzmán
The aim of the present work was to determine whether treatment with melatonin modifies the reproductive response of female goats experiencing increasing or decreasing body weight (BW)/body condition score (BCS) when introduced to bucks displaying springtime sexual activity. During natural anoestrus, 53 does were isolated from bucks for a period of 42days and distributed into two groups: 1) low BW/low BCS animals (N=24) (LLg group), which were fed 1.9 times their maintenance requirements so they would experience increasing BW and BCS; and 2) high BW/high BCS animals (N=29) (HHl group), which were fed 0...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28148467/comparison-of-fertility-of-liquid-or-frozen-semen-when-varying-the-interval-from-cidr-removal-to-insemination
#19
Brittany N Richardson, Erin L Larimore, Julie A Walker, Matthew D Utt, J Mel DeJarnette, George A Perry
Cryopreservation allows for long-term storage of semen; however, it leads to damage of sperm that may result in complete loss of viability or changes that possibly decrease sperm functionality. Liquid semen is not exposed to these stressors and may result in a longer lifespan in the female reproductive tract, thus increasing the range in timing of insemination without affecting fertility. The objective of this study was to compare fertility of liquid and frozen semen when varying the interval from CIDR removal to AI using the 7-day CO-Synch+CIDR protocol for synchronization of time of estrus...
March 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28139300/identification-of-a-novel-12-bp-insertion-deletion-indel-of-ips-related-oct4-gene-and-its-association-with-reproductive-traits-in-male-piglets
#20
Fa Ren, Shuai Yu, Rui Chen, Xiaoyan Lv, Chuanying Pan
As a key factor of cellular reprogramming, Oct4 is one of vital transcription factors for induced pluripotent stem cells (iPSCs). Loss of its function or deletion causes apoptosis in primordial germ cells (PGCs), which affect reproductive traits in mammals. In this study, a novel 12-bp insertion/deletion (indel) polymorphism (NC_010449:g.2759-2760insGGTTTTTGTCTA) within the Oct4 gene was identified in 442 pigs of Large White (LW) and Landrace (LD) breeds, showing three genotypes designated as II, ID, and DD...
March 2017: Animal Reproduction Science
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