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Animal Reproduction Science

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https://www.readbyqxmd.com/read/28416376/seminal-plasma-removal-by-density-gradient-centrifugation-is-superior-for-goat-sperm-preservation-compared-with-classical-sperm-washing
#1
J Santiago-Moreno, M C Esteso, C Castaño, A Toledano-Díaz, J A Delgadillo, A López-Sebastián
Seminal plasma removal is routine in goat sperm cryopreservation protocols. The classical washing procedure designed to accomplish this usually leaves the pellet resulting from use of this procedure contaminated with dead sperm, debris, and cells other than sperm. This contamination negatively affects viability of sperm after cryopreservation. The present research was conducted to compare the effect on chilled and frozen-thawed goat sperm of the classical washing method to that of a selective washing method involving density gradient centrifugation (DGC)...
April 9, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28431863/the-myometrial-contractility-during-late-pregnancy-in-dairy-cows-in-vitro
#2
L Górriz-Martín, S E Ulbrich, M Schmicke, G Hirsbrunner, C Keller, N Yücesoy, C Pfarrer, H Bollwein, M Heppelmann
This study aimed to investigate the in vitro contractility of the myometrium and its relationship to the blood concentrations of estradiol-17β (E2β), progesterone (P4), 15-keto-13,14-dihydro-PGF2α (PGFM) and ionised calcium (Ca(2+)) prior to tissue harvest in 12 healthy Holstein-Friesian cows in late pregnancy. Three circular (CM) and 3 longitudinal myometrial (LM) strips were dissected during a caesarean section and mounted in an organ bath containing modified Krebs solution (KS). The spontaneous contractility was recorded during five 30-min time periods (T1 to T5), after which the strips were exposed to increasing concentrations of oxytocin (OT; 10(-10)-10(-7)M), a natural PGF2α-analogue (PGF; 10(-7)-10(-4)M) and KS (Cont) for four 30-min time periods (T6 to T9)...
April 7, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28433506/heifers-express-g-protein-coupled-receptor-61-in-anterior-pituitary-gonadotrophs-in-stage-dependent-manner
#3
Kiran Pandey, Onalenna Kereilwe, Vitaliano Borromeo, Hiroya Kadokawa
Gonadotropin-releasing hormone receptors (GnRHRs) colocalize with insulin and glucocorticoid receptors in lipid rafts of the gonadotroph plasma membrane, where they facilitate downstream signaling. We recently found that orphan G-protein-coupled receptor (GPR)61 is expressed in the anterior pituitary (AP) of heifers, leading us to speculate that GPR61 colocalizes with GnRHR in the plasma membrane of gonadotroph and is expressed at specific times of the reproductive cycle. To test this hypothesis, we examined the coexpression of GnRHR, GPR61, and either luteinizing hormone (LH) β subunit or follicle-stimulating hormone (FSH) β subunit in AP tissue and cultured AP cells by immunofluorescence microscopy...
April 4, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28416377/comparison-between-two-estradiol-progesterone-based-protocols-for-timed-artificial-insemination-in-blocks-in-lactating-nelore-cows
#4
L F M Pfeifer, N A Castro, P M A Neves, J P Cestaro, A Schneider
The aim of this study was to compare the use of artificial insemination in time blocks (Artificial Insemination Blocks, AIB) using an 8 and 9 d estradiol-progesterone based protocol. In this experiment, lactating Nelore cows (n=253) were subjected to two estradiol-progesterone based TAI protocols. On the morning of Day 10 (8d group, n=124) or Day 11 (9d group, n=129), cows were examined by ultrasonography to evaluate the diameter of the preovulatory follicle and were inseminated once at one of the following time points, according to the diameter of the pre-ovulatory follicle (POF): Block 0 (POF≥15mm, TAI 0h after conventional TAI), Block 1 (POF 13...
April 4, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28389048/postnatal-developmental-of-neuromedin-s-and-its-receptor-in-the-male-xiaomeishan-pig-reproductive-axis
#5
Zhiyu Ma, Yangyang Zhao, Yuan Yao, Zhihai Lei, Mengmeng Jin, Xiang Li, Cuicui Jia, Zheng Zhang, Xiaoliang Li, Juan Su
Neuromedin S (NMS) has been identified as an endogenous ligand for FM-3/GPR66 and FM-4/TGR-1 which are NMU receptors NMUR1 and NMUR2, respectively. The NMS molecule is present in some peripheral tissues and the central nervous system (CNS), and it had been documented that NMS has fundamental and important roles in multiple physiological functions and processes such as circadian rhythm, energy balance, feeding behavior, stress responses and reproduction. The possible role of NMS in sexual development postnatally, however, is still obscure...
April 2, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28431864/toward-an-integrative-and-predictive-sperm-quality-analysis-in-bos-taurus
#6
J L Yániz, C Soler, C Alquézar-Baeta, P Santolaria
There is a need to develop more integrative sperm quality analysis methods, enabling researchers to evaluate different parameters simultaneously cell by cell. In this work, we present a new multi-parametric fluorescent test able to discriminate different sperm subpopulations based on their labeling pattern and motility characteristics. Cryopreserved semen samples from 20 Holstein bulls were used in the study. Analyses of sperm motility using computer-assisted sperm analysis (CASA-mot), membrane integrity by acridine orange-propidium iodide combination and multi-parametric by the ISAS(®)3Fun kit, were performed...
March 31, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28416375/automatic-and-manual-doppler-velocimetry-measurements-of-the-uterine-artery-in-pregnant-ewes
#7
Renato Travassos Beltrame, Lucas Buss Littig, Carolina Covre, Amanda de Barros Martins, Celia Raquel Quirino, R L D Costa
We aimed to compare measurements of speed and blood flow parameters obtained manually and automatically in the uterine arteries of pregnant ewes. Eighteen Santa Inês ewes, confirmed to be pregnant, were followed every 2 weeks until parturition. The systolic peak (PS), end diastolic velocity (ED), maximum and average speed in the cardiac cycle (TAMAX and TAMEAN, respectively), pulsatility index (PI), resistance index (RI) and the systole/diastole (S/D) ratio and body flow volume (BFV) was measured. We began the wave evaluation automatically, then the observer manually marked the beginning and end of the wave...
March 31, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28412080/reproductive-parameters-of-critically-endangered-european-mink-mustela-lutreola-in-captivity
#8
Kairi Kiik, Tiit Maran, Kristel Nemvalts, Siiri-Lii Sandre, Toomas Tammaru
Founding captive populations is often the last chance for saving endangered species from extinction. Ensuring successful reproduction is typically most critical for the maintenance of captive populations, with purposeful selection of individuals for breeding being one of the crucial aspects. Comparable cross-species data on the determinants of reproduction success are most useful for solving problems in captive species programs. In the present study, we provide an overview of a 20-year captive breeding program of the critically endangered European mink...
March 31, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28385397/maternal-age-modulates-the-effects-of-early-pregnancy-l-proline-supplementation-on-the-birth-weight-of-piglets
#9
P Gonzalez-Añover, A Gonzalez-Bulnes
Previous results obtained in gilts maintained under experimental conditions suggest that amino acid supplementation during pregnancy may be a promising strategy for diminishing the incidence of embryo losses and low birth-weight newborn. The current study evaluated the effects of a short-term supplementation with L-proline, around implantational stages, on litter size and birth-weight of piglets in sows of different parities maintained under commercial farm conditions. There were no significant effects in mature sows with three or more parities, but the supplementation improved the reproductive efficiency of the high-prolific first-parity sows and of all the sows at second-parity...
March 30, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28385396/monitoring-menstrual-cycle-gestation-and-lactation-by-measuring-urinary-oestradiol-and-progesterone-in-the-captive-golden-snub-nosed-monkey-rhinopithecus-roxellanae
#10
Wei-Zhen Chen, Yue Li, Hui-Liang Yu, Hui Yao, Xiang Li, Li Han, Chang-Min Hu, Jia-Jun Xiong, Dong-Ming Liu, Ming-Xing Ding, Jian-Guo Chen
The golden snub-nosed monkey is an endangered species and study of its reproductive physiology is crucial for the species' breeding programs. Urine samples (770) from 5 mature female golden snub-nosed monkeys were collected in the Shengnongjia Nature Reserve between October 2013 and December 2014 to monitor their menstrual cycle, gestation, and lactation. The concentrations of oestradiol (E2) and progesterone (P4) in the samples were measured by Chemiluminescent Microparticle Immunoassay (CMIA), and the hormone concentrations were indexed to creatinine levels to compensate for differences in water content...
March 30, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28385395/cytoplasmic-droplet-acting-as-a-mitochondrial-modulator-during-sperm-maturation-in-dogs
#11
D S R Angrimani, J D A Losano, C F Lucio, G A L Veiga, F C Landim, M Nichi, C I Vannucchi
Motility acquisition during sperm maturation and passage through the epididymis is closely related to mitochondrial function and appears to occur in parallel with cytoplasmic droplet (CD) migration. However, such mechanism remains unclear in dogs. Thus, the aim of this study was to characterize the influence of sperm CD in the mitochondrial functionality during epididymal sperm maturation in dogs. Twenty-one adult dogs were submitted to elective bilateral orchiectomy. Testicles were stored for 18-24h at 5°C and epididymal sperm samples were then collected from different segments of the epididymis (caput, corpus and cauda)...
March 30, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28413155/effects-of-natural-environment-on-reproductive-histo-morphometric-dynamics-of-female-dromedary-camel
#12
Hafiz Muhammad Ali, Anas Sarwar Qureshi, Riaz Hussain, Giorgia Urbinati, Mohammad Zahid Mustafa, Farah Ali, Abdul Manan, Liliane Massaad-Massade
Camel shows a seasonal breeding pattern with enhanced reproductive activity during the period of low climatic temperature, high rainfall and better food conditions. Therefore, the study was conducted to explore the underlying seasonal effects on histological dimensions of reproductive tract in adult female one-humped camel (Camelus dromedarius) kept in the natural environment of Pakistan. A total 25 reproductive tracts were collected during spring, summer, autumn and winter seasons and were analysed for histo-morphometric parameters during different environmental conditions...
March 29, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28389047/effects-of-ecg-and-progesterone-on-superovulation-and-embryo-production-in-wood-bison-bison-bison-athabascae
#13
J Manuel Palomino, Miriam P Cervantes, Murray R Woodbury, Reuben J Mapletoft, Gregg P Adams
Experiments were done to determine if inclusion of eCG and progesterone in the superstimulation protocol will increase the ovarian response and embryo production in wood bison, and to provide preliminary information regarding the effect of season. In Experiment 1 (anovulatory season), bison (n=26) were synchronized by follicular ablation (Day -1) and given FSH on Days 0 and 2, and assigned to 3 groups: Progesterone (Days 0-4), eCG (Day 3), or progesterone+eCG. On Day 5, bison were given hCG and inseminated 12 and 24h later...
March 29, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28400072/notch-signaling-pathway-promotes-the-development-of-ovine-ovarian-follicular-granulosa-cells
#14
Jiongjie Jing, Xiaolong Jiang, Jianwei Chen, Xiaolei Yao, Miaomiao Zhao, Pengfei Li, Yangyang Pan, Youshe Ren, Wenzhong Liu, Lihua Lyu
The Notch signaling pathway regulates cell proliferation, differentiation and apoptosis involved in development of the organs and tissues such as nervous system, cartilage, lungs, kidneys and prostate as well as the ovarian follicles. This study aimed to investigate the mRNA expression and localization of NOTCH2, as the key factor in Notch signaling pathway. This was determined by PCR, real-time PCR and immunohistochemistry. Additionally, the effects of inhibiting Notch signaling pathway with different concentrations (5μM, 10μM and 20μM) of N-[N-(3, 5-Difuorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT), an inhibitor of Notch signaling pathway, on ovine granulosa cells was determined in vitro by detecting estradiol production using enzyme linked immunosorbent assay and expressions of the genes related to the cell cycle and apoptosis using real-time polymerase chain reaction (PCR)...
March 28, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28392308/effects-of-grazing-tall-fescue-containing-ergot-alkaloids-on-bull-sperm-cryopreservation
#15
C R Burnett, W C Bridges, S L Pratt
There are many positive agronomic traits that make tall fescue a desirable forage, however, reduced fertility rates are reported for beef cattle grazing pasture containing the ergot alkaloid-producing endophyte, Epichloë coenophiala. The objective of this study was to assess the influence of consumption tall fescue containing ergot alkaloids on sperm physiology as measured by survival of sperm following cryopreservation. Yearling Angus bulls (n=25), having passed a breeding soundness exam (BSE), were assigned to one of two treatments accounting for body weight (BW) and body condition score (BCS)...
March 28, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28385394/effect-of-injectable-trace-mineral-complex-supplementation-on-development-of-ovarian-structures-and-serum-copper-and-zinc-concentrations-in-over-conditioned-holstein-cows
#16
Juan González-Maldonado, Raymundo Rangel-Santos, Raymundo Rodríguez-de Lara, Oswaldo García-Peña
This study evaluated the effect of injecting trace minerals on reproductive performance in over-conditioned Holstein cows before synchronized estrus. Multiparous non-lactating, over-conditioned repeat breeder cows (n=20) were assigned randomly to one of two treatments: 1) control (n=10), and 2) supplementation with an injectable trace mineral complex 25days before expected synchronized estrus (n=10). Follicular waves were synchronized by intravaginal insertion of a CIDR for eight days and an intramuscular (i...
March 28, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28385398/reproductive-parameters-of-dairy-goats-after-receiving-two-doses-of-d-cloprostenol-at-different-intervals
#17
A L R S Maia, F Z Brandão, J M G Souza-Fabjan, M F A Balaro, M E F Oliveira, O Facó, J F Fonseca
This study evaluated the efficiency of two d-cloprostenol injections at different intervals on the reproductive parameters of dairy goats. Trial 1 comprised 54 goats allocated to receive two 37.5μg d-cloprostenol doses at intervals of seven (T7, n=19), 10 (T10, n=18), and 11.5 (T11.5, n=17) days. Trial 2 comprised 62 goats allocated to receive injections at T7 (n=30) and T11.5 (n=32). Ultrasonography was done and blood was collected just before d-cloprostenol injections. After the second dose, goats were artificially inseminated (AI) with frozen-thawed semen at 18-24h (Trial 1) or at 10-24h (adjusted according to the time of estrus onset in Trial 2) after estrus detection...
March 23, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28366279/chemical-sterilisation-of-animals-a-review-of-the-use-of-zinc-and-cacl2-based-solutions-in-male-and-female-animals-and-factors-likely-to-improve-responses-to-treatment
#18
REVIEW
John Cavalieri
Chemical sterilisation can be used as an alternative to surgical castration in some circumstances. This review focuses on responses to treatment with zinc- or CaCl2-based chemosterilants, factors that have affected treatments and their potential use to sterilise female cattle. Successful treatment with a low incidence of adverse side effects in male animals has occurred with the use of zinc gluconate (ZG), neutralised in arginine and a 20% solution of CaCl2 in ethanol. Injection technique plays an important role in success...
March 18, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28342689/induction-of-out-of-season-egg-laying-by-artificial-photoperiod-in-yangzhou-geese-and-the-associated-endocrine-and-molecular-regulation-mechanisms
#19
Huanxi Zhu, Xibing Shao, Zhe Chen, Chuankun Wei, Mingming Lei, Shijia Ying, Jianning Yu, Zhendan Shi
This study was carried out to induce out-of-season breeding, in the summer, and to achieve high reproductive performance using artificial photoperiod manipulation in the long-day breeding Yangzhou goose. Young geese were subject to a two-phase short-to-long (group A) or a three-phase (long-short-long; group B) photoperiod program February through October. Egg-laying was induced to start similarly in both groups in May, increased to a peak level in July, and then decreased gradually through to October. The peak and post-peak laying rates were higher with the three-phase than with the two-phase program...
March 18, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28341393/cell-specific-immuno-localization-of-progesterone-receptor-alpha-in-the-rabbit-ovary-during-pregnancy-and-after-parturition
#20
Mahmoud Abd-Elkareem
Progesterone receptor alpha (PRA) has a central coordinator role in the ovarian functions in mammals. The aim of this study was to investigate the immunolocalization of PRA in the rabbit ovary during pregnancy and after parturition. The rabbit ovary during pregnancy and after parturition had moderate cytoplasmic and moderate to intense nuclear PRA immunostaining in the ovarian surface epithelial cells, stromal cells and interstitial gland cells. The PRA was also present in granulosa cells and theca interna cells of the growing, small antral and mature Graafian follicles...
March 18, 2017: Animal Reproduction Science
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