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Animal Reproduction Science

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https://www.readbyqxmd.com/read/30384994/polymorphism-of-melatonin-receptor-mtnr1a-gene-and-its-association-with-seasonal-reproduction-in-water-buffalo-bubalus-bubalis
#1
Pankaj Gunwant, Anand Kumar Pandey, Aman Kumar, Inderjeet Singh, Sandeep Kumar, J B Phogat, Vinay Kumar, C S Patil, Piyush Tomar, Sunil Kumar, Ankit Magotra
The water buffalo have a seasonal pattern of reproduction with decreased sexual activity during the longer photoperiod. The present study was designed to identify a single nucleotide polymorphism (SNP) in the MTNR1 A gene and its association with seasonal reproduction and reproductive characteristics in Murrah buffalo cows. The 812 bp fragment encompassing exon II of the MTNR1 A gene was amplified from genomic DNA of 190 pluriparous Murrah buffalo cows. Amplified PCR products from 12 samples were subjected to custom sequencing of both ends (5' and 3' ends)...
October 26, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30384993/extensive-management-conditions-do-not-modify-the-frequency-of-short-ovulatory-cycles-in-progesterone-treated-does-exposed-to-sexually-active-males
#2
J D Andrade-Esparza, L A Espinoza-Flores, H Hernández, P Chemineau, M Keller, J A Delgadillo
Most goats exposed to males in confined conditions have short ovulatory cycles. The frequency of these cycles can be reduced with a progesterone treatment prior to the introduction of males. The objective of this study was to determine whether extensive management conditions modify the frequency of short ovulatory cycles in progesterone-treated does exposed to photostimulated males. One group of does remained in extensive management conditions and grazed daily from 10:00 to 18:00 h; two other groups were confined separately in shaded pens, and fed alfalfa hay...
October 25, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30340853/ptgfr-activation-promotes-the-expression-of-ptgs-2-and-growth-factors-via-activation-of-the-pkc-signaling-pathway-in-bovine-endometrial-epithelial-cells
#3
Long Gao, Ruifeng Gao, Wei Mao, Bo Liu, Shuangyi Zhang, Duri Tahala, Changqi Fu, Yuan Shen, Jindi Wu, Yang Deng, Qianru Li, Jinshan Cao
The endometrium of domestic animals has a remarkable capacity to self-repair. Prostaglandin F2α (PGF2α ) is one of the major prostaglandins secreted from the endometrium. The role of PGF2α in endometrial repair, however, is still unknown. In the present study, it was investigated whether prostaglandin F2α receptor (PTGFR) activation could induce expression of prostaglandin-endoperoxide synthase 2 (PTGS-2) and growth factors associated with endometrial repair via activation of protein kinase C (PKC) signaling in endometrial epithelial cells (bEECs) of cattle...
October 14, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30340855/azoospermia-in-male-dromedary-clinical-findings-testicular-biopsy-serum-follicle-stimulating-hormone-and-seminal-biomarkers
#4
Derar Refaat Derar, Ahmed Ali, Moustafa M Zeitoun, Fahd Al-Sobayil
Azoospermia is not an uncommon infertility problem in the male dromedary (Camelus dromedarius). Azoospermia was investigated via clinical findings, testicular biopsy as well as the evaluation of follicle stimulating hormone (FSH), concentration of camel testis protein (TEX101) and camel epididymis-specific extracellular matrix protein (ECM1) in seminal fluids. Azoospermic male camels (AZOO group, n = 28) that had been detected to be infertile as a result of lack of resulting pregnancies after repeated mating's for at least one season were included in this study...
October 11, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30340854/b-mode-and-contrast-enhanced-ultrasonography-of-the-mammary-gland-during-the-estrous-cycle-of-dogs
#5
Katrien Vanderperren, Jimmy H Saunders, Elke Van der Vekens, Eline Wydooghe, Hilde de Rooster, Luc Duchateau, Emmelie Stock
Pathology of the mammary gland is a common health issue in dogs and includes neoplasia, cysts, inflammation and infection. The use of the B-mode (US) and contrast-enhanced (CEUS) ultrasonography may aid in the diagnosis. Previous studies are currently lacking of the ultrasonic images of the mammary gland of healthy bitches in different stages of the estrous cycle and associated normal blood perfusion patterns. The purpose, therefore, was to describe the normal B-mode US and CEUS images of the mammary gland and inguinal lymph node, in six intact female beagles during five different stages of the estrous cycle (proestrus, estrus, early and late diestrus and anestrus)...
September 13, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30201256/effects-of-short-term-intravaginal-progestagens-on-the-onset-and-features-of-estrus-preovulatory-lh-surge-and-ovulation-in-sheep
#6
Paula Martinez-Ros, Susana Astiz, Empar Garcia-Rosello, Alejandro Rios-Abellan, Antonio Gonzalez-Bulnes
In the present study, there was a comparison among classical long-term progestagen (fluorogestone acetate) protocols for synchronization of estrus and ovulation (14 days; group FGA14, n = 9 ewes) and short-term protocols based on 7 days of progestagen treatment plus a dose of prostaglandin F2α at either insertion (PG-FGA7, n = 11) or removal (FGA7-PG, n = 12). There were no significant differences in the ovulation rate and progesterone secretion among treatments. The FGA7-PG group, however, had a similar percentage of ewes expressing estrous behavior than the group FGA14 (90...
August 31, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30174098/nulliparous-and-primiparous-cows-produce-less-fertile-female-offspring-with-lesser-concentration-of-anti-m%C3%A3-llerian-hormone-amh-as-compared-with-multiparous-cows
#7
V Akbarinejad, F Gharagozlou, M Vojgani, M M Bagheri Amirabadi
Nutritional partitioning to fetal development differ among nulliparous, primiparous and multiparous cows, leading to birth of smaller calves to nulliparous and primiparous than multiparous dams. The prenatal nutritional state could influence ovarian reserve and fertility in the adult offspring. The effect of maternal parity on ovarian reserve and reproduction of female offspring, however, has not been investigated in cattle. Accordingly, a retrospective research was conducted to study reproductive variables in nulliparous- (n = 310), primiparous- (n = 236) and multiparous-born (n = 323) female offspring during the first four lactation periods in dairy cows...
August 28, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30174097/genome-wide-transcriptome-profiling-in-ovaries-of-small-tail-han-sheep-during-the-follicular-and-luteal-phases-of-the-oestrous-cycle
#8
Han Wang, Xiangyun Li, Rongyan Zhou, Jianzhong Xi, Yanhui Wei, Lanhui Li, Zhenhong Zhang
Ovarian function, the control of which is predominantly integrated by the hypothalamic-pituitary-ovarian axis, is pivotal for maintaining reproductive efficiency in sheep. To understand the regulatory mechanism of the follicular-luteal phase transition in prolific sheep, the genome-wide expression patterns of microRNAs and genes in ovarian tissue of Small Tail Han sheep were examined during the follicular and luteal phases of the oestrous cycle. Differentially expressed genes (n = 450) were identified and a total of 139 known and 72 novel miRNAs were identified in the two libraries...
August 27, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30177478/hydrometra-in-dairy-goats-ultrasonic-variables-and-therapeutic-protocols-evaluated-during-the-reproductive-season
#9
A L R S Maia, F Z Brandão, J M G Souza-Fabjan, M O Veiga, M F A Balaro, L G B Siqueira, O Facó, J F Fonseca
Hydrometra is characterized by the accumulation of fluid within the uterus due to the persistence of corpus luteum. The diagnosis of this disorder occurs with an ultrasonic exam. This study evaluated uterine drainage and fertility rates in goats after the use of d-cloprostenol in association or not with Gonadotropin-releasing hormone (GnRH) treatment. Twenty Saanen goats, diagnosed with hydrometra, received three 37.5-μg doses of d-cloprostenol laterovulvarly at 10-day intervals. On D5, the goats were assigned into two groups receiving 1 mL of GnRH or saline solution intramuscularly...
August 26, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30172606/effects-of-maternal-obesity-on-maternal-and-fetal-plasma-concentrations-of-adiponectin-and-expression-of-adiponectin-and-its-receptor-genes-in-cotyledonary-and-adipose-tissues-at-mid-and-late-gestation-in-sheep
#10
S R Fensterseifer, K J Austin, S P Ford, B M Alexander
Adiponectin potentially influences fetal weight by altering insulin signaling and trans-placental amino acid and glucose transporters. The objective of this study was to determine how maternal obesity influences maternal and fetal plasma concentrations of adiponectin, expression of fetal adiponectin, its receptors, and adipogenic genes at mid- and late-gestation. Blood samples and tissues were collected from obese and control multiparous pregnant ewes at day 75 or 135 of gestation. Although day of gestation or maternal obesity did not influence (P >  0...
August 26, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30172604/b-mode-ultrasonography-and-ecobiometric-parameters-for-assessment-of-embryonic-and-fetal-development-in-sheep
#11
Victor José Correia Santos, Mariana Garcia Kako Rodriguez, Priscila Del Aguila da Silva, Renata Sitta Gomes Mariano, Augusto Ryonosuke Taira, Vivian Tavares de Almeida, Ricardo Andrés Ramirez Uscategui, Ricardo Perecin Nociti, Pedro Paulo Maia Teixeira, Marcus Antonio Rossi Feliciano, Wilter Ricardo Russiano Vicente
The aim in this study was to evaluate the maternal-fetal development during physiological gestation in sheep by means of ultrasonography and to predict the gestational age by mathematical formulae. Thirty Santa Inês sheep were included. Evaluations were performed weekly from the 3rd to 21st gestational week. The Shapiro test was used to determine normality of the data, and Spearman test was used for determining correlations of real or transformed measurements as gestation advanced. If there was a significant difference detected, variable adjustments and gestational weeks were tested using regression models...
August 26, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30166078/effect-of-seasonality-on-testicular-blood-flow-as-determined-by-color-doppler-ultrasonography-and-hormonal-profiles-in-shiba-goats
#12
Haney Samir, Paul Nyametease, Kentaro Nagaoka, Gen Watanabe
The objectives of this study were to determine if there is a seasonal pattern of testicular blood flow in male Shiba goats, and ascertaining whether or not there is a relationship among changes in testicular hemodynamics and circulating testosterone (T) and estradiol (E2). Twelve sexually mature male Shiba goats (Capra hircus), aged 19.5 ± 2.5 months were used in this study. Monitoring of the changes in testicular blood flow was performed once a week for 1 year, using color spectral Doppler ultrasonography...
August 23, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30154034/role-of-fgf9-in-sheep-testis-steroidogenesis-during-sexual-maturation
#13
Xiaoxiao Gao, Xiaolei Yao, Hua Yang, Kaiping Deng, Yixuan Guo, Tingting Zhang, Guomin Zhang, Feng Wang
Fibroblast growth factor 9 (FGF9) is an important signaling molecule in early gonadal development. Hu sheep are noted for reproductive precociousness and fertility. The present study was conducted to investigate the gene expression and functions of FGF9 in ovine testis steroidogenesis during sexual maturity. A 874 bp cDNA fragment of FGF9 was detected that included a 627 bp coding sequence, encoding 208 amino acids. The FGF9 amino acid sequence of sheep had high homology with this molecule of other mammalian species...
August 22, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30150093/reduction-of-dissolved-oxygen-in-semen-extender-with-nitrogen-gassing-reduces-oxidative-stress-and-improves-post-thaw-semen-quality-of-bulls
#14
Abhishek Kumar, J K Prasad, A R Mustapha, Beigh Yaqoob Amin, Omer Din, R Katiyar, G K Das, N Srivastava, Ajay Kumar, M R Verma, S K Ghosh
The objective of the present study was to investigate the effects of two different concentrations of dissolved oxygen (DO, 4 and 8) ppm in the extender on oxidative stress affecting plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), and deoxyribonucleic acid (DNA) damage of bull spermatozoa following cryopreservation. For the experiment, nitrogen (N2 ) gassing of the extender for varied time intervals yielded extender with DO concentration of 4 ppm and 8 ppm (Groups II and III, respectively)...
August 22, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30146093/elastographic-and-echotextural-characteristics-of-foetal-lungs-and-liver-during-the-final-5-days-of-intrauterine-development-in-dogs
#15
Ana Paula Rodrigues Simões, Marcus Antonio Rossi Feliciano, Marjury Cristina Maronezi, Ricardo Andres Ramirez Uscategui, Pawel Mieczyslaw Bartlewski, Vivian Tavares de Almeida, David Oh, Paloma do Espírito Santo Silva, Liege Cristina Garcia da Silva, Wilter Ricardo Russiano Vicente
Objective was to evaluate the echotexture and characteristics during terminal development of canine foetal respiratory and hepatic systems through elastographic examinations. Fifteen pregnant bitches were evaluated by ultrasonography twice daily, from the 53rd gestational day until whelping, and images obtained from 120 to 0 h before parturition were analysed. Images of foetal lungs and liver were recorded and then used for computer-assisted analyses to determine quantitative attributes. Acoustic Radiation Force Impulse (ARFI) elastographic of internal organs were classified as 'soft' (white areas) or 'hard' (dark areas) and quantitative analyses determined the mean shear wave velocities (SWV) of foetal lungs and liver...
August 22, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30322674/early-embryo-development-number-quality-and-location-and-the-relationship-with-plasma-progesterone-in-dogs
#16
S Miranda, N Carolino, H Vilhena, R Payan-Carreira, R M L N Pereira
This research assessed the developmental stages and morphological quality of dog embryos collected during different stages of pregnancy as well as the relationship with serum progesterone recorded at insemination and embryo collection. Embryos were collected from 23 young mature bitches, that had been inseminated with fresh semen 3-6 days after the LH surge (day 0). Embryo flushing was performed on pregnancy days 8-11 (Group 1), 12-15 (Group 2), or 16-20 (Group 3). The location, number and morphological characteristics of the embryos were evaluated...
November 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30318312/ovarian-transcriptomic-analysis-reveals-the-alternative-splicing-events-associated-with-fecundity-in-different-sheep-breeds
#17
Xiangyang Miao, Qingmiao Luo, Huijing Zhao, Xiaoyu Qin
Alternative splicing (AS) is one of the most common mechanisms that accounts for the greater macromolecular and cellular complexity of higher eukaryotic organisms. This study focused on the splicing events in the ovaries of different sheep breeds, namely the Han and Dorset breeds. Of the groups studied, cassette splicing events accounted for the maximum number of the AS events with significant differences, whereas the splicing events that were mutually exclusive with introns accounted for the smallest proportion of splicing events...
November 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30301622/effect-of-reduced-glutathione-gsh-supplementation-to-tris-egg-yolk-extender-on-chilled-semen-variables-of-dogs
#18
Anne Hoeg Andersen, Mette Thinnesen, Klaus Failing, Sandra Goericke-Pesch
One reason for reduced longevity of chilled dog semen is oxidative stress. The antioxidant glutathione (GSH) improves viability of frozen-thawed dog sperm, but its effect on chilled dog semen has not been investigated. An experiment consisting of two parts was performed: Sperm rich fractions, SRF, were split, diluted with a Tris-egg yolk (TEY) extender containing 0, 5 or 10 mM GSH and stored at 4 °C for 10 days (Part 1; n = 19) or 4 days (Part 2; n = 11), respectively. For Part 1 of the study, percentage (%) of motile, viable, morphologically abnormal spermatozoa and % acrosomal deviations were assessed on days 0, 1, 2, 4 and 10 after dilution...
November 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30301621/sperm-quality-in-wild-caught-and-farmed-males-of-the-european-eel-anguilla-anguilla
#19
L Locatello, D Bertotto, R Cerri, A Parmeggiani, N Govoni, A Trocino, G Xiccato, O Mordenti
Sperm density and performance of wild and farmed male European eels were evaluated to investigate the effect of maintenance in captivity on ejaculate quality. Hormonal stimulation (with human chorionic gonadotropin) lasted for 17 weeks. Different origins of the male European eels partially influenced the quality of the ejaculates. Indeed, wild animals (i.e., collected at a natural site) had greater sperm longevity during the hormonal stimulation, whereas there was no significant effect of the origin on sperm density, spermatocrit, the percentage of motile sperm, and plasma testosterone concentrations...
November 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30301620/effect-of-length-of-time-post-mortem-on-quality-and-freezing-capacity-of-cantabric-chamois-rupicapra-pyrenaica-parva-epididymal-spermatozoa
#20
M Álvarez-Rodriguez, M Álvarez, L Anel-López, C Guerra, C A Chamorro, L Anel, P de Paz, F Martínez-Pastor
Genome Resource Banks are keystones in the ex-situ conservation of wild species. Post-mortem (PM) collection of epididymal spermatozoa is an opportunistic and valuable source of germplasm, the time from the death of the animal limits its use. Seeking to improve germplasm preservation strategies for the chamois (Rupicapra sp.), the effect of PM time on epididymal sperm quality and freezability was studied using the Cantabrian chamois. Samples were classified according to PM collection time, up to 216 h (refrigerated), and cryopreserved (Tris-citric acid-fructose, 430 mOsm/kg, 15% egg yolk, 8% glycerol; freezing at -20 °C/min)...
November 2018: Animal Reproduction Science
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