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Pathology, Research and Practice

Hasan Mujtaba, Ying Wang, Yixin Duan, Meng Cao, Nana Zhang, Iffat Batool, Ali Murtaza, Xiaoli Chen, Yili Wang
BACKGROUND: The expected corresponding increase in tonsillar human papillomavirus (HPV) infection associated with increasing incidence of oropharyngeal squamous cell carcinoma (OPSCC) substantiate the evaluation of normal tonsillar tissue in different population. The epidemiology of HPV in tonsillar tissue varies geographically. This study evaluated samples from two countries to determine the prevalence in respective samples. OBJECTIVE: To characterize HPV infection in non-malignant tonsillar tissue from Shaanxi, China (herein after referred to as China) and Punjab, Pakistan (herein after referred to as Pakistan)...
September 6, 2018: Pathology, Research and Practice
J D Pallua, A Brunner, B Zelger, R Stalder, S H Unterberger, M Schirmer, M C Tappert
New developments in Mid-infrared microscopic imaging instrumentation and data analysis have turned this method into a conventional technique. This imaging method offers a global analysis of samples, with a resolution close to the cellular level enabling the acquisition of local molecular expression profiles. It is possible to get chemo-morphological information about the tissue status, which represents an essential benefit for future analytical interpretation of pathological changes of tissue. In this review, we give an overview of Mid-infrared microscopic imaging and its applications in clinical research...
September 4, 2018: Pathology, Research and Practice
Andres M Acosta, Erica Vormittag, Mohamed R Haroon Al Rasheed, Asma Sharif, Khin-Su Mon, Andre Kajdacsy-Balla, Gayatry Mohapatra
Intraductal carcinoma of the prostate(IDCP) is defined as a solid or cribriform neoplastic growth confined to ducts and acini, with preservation of the basal cell layer. Since IDCP can often present tumor necrosis (TN), it should be distinguished from Gleason 5 (GP5) invasive adenocarcinoma for staging and clinical purposes. In the present study we reviewed 344 radical prostatectomies performed at our institution and selected all cases with either >5% GP5 or IDCP for assessment of TN on histology slides (n = 59)...
August 29, 2018: Pathology, Research and Practice
Yehong Li, Zhongwen Yang
OBJECTIVES: Prostate adenocarcinoma (PRAD) is the most common cancer in men. The aim of this study was to reveal the critical long non-coding RNA (lncRNAs), microRNA (miRNAs) and mRNAs involved in the pathogenesis of PRAD. METHODS: The level 3 mRNA and miRNA sequencing data of PRAD were downloaded from The Cancer Genome Atlas database. Using the edgeR package of R, the differentially expressed mRNAs (DEGs), lncRNAs (DE-lncRNAs) and miRNAs (DE-miRNAs) between PRAD and normal tissues were screened...
August 29, 2018: Pathology, Research and Practice
Caterina Vicentini, Cinzia Cantù, Davide Antonello, Michele Simbolo, Andrea Mafficini, Claudio Luchini, Borislav Rusev, Antonio Benito Porcaro, Roberto Lacovelli, Matteo Fassan, Vincenzo Corbo, Matteo Brunelli, Walter Artibani, Aldo Scarpa, Rita T Lawlor
INTRODUCTION: One of the most common sites of distant metastasization of prostate cancer is bone, but to date reliable biomarkers able to predict the risk and timing of bone metastasization are still lacking. PATIENTS AND METHODS: Surgically resected paraffin embedded samples from 12 primary prostate cancers that developed metachronous bone metastasis at different time points were studied (six cases within 2 years, six cases after 5 years from surgery). A targeted next-generation DNA and RNA sequencing able to assess simultaneously mutations, copy number alterations and fusion events of multiple genes was used...
August 29, 2018: Pathology, Research and Practice
Yitong Zhang, Jing Liu, Yanyun Ma, Jingjie Wang, Jie Zhu, Jie Liu, Jun Zhang
Liver fibrosis is the underlying cause of cirrhosis and liver failure in any type of chronic liver injury. However, the function of lncRNAs in liver fibrosis is largely unknown. In this study, we performed transcriptome sequencing of CCl4 induced mouse fibrotic liver tissues and normal liver tissues, and found 118 lncRNAs and 1921 mRNAs were significantly up-regulated, whereas 59 lncRNAs and 1504 mRNAs were down-regulated in fibrotic livers. Gene ontology analysis revealed that the differentially expressed lncRNAs are implicated in cell junction, cell differentiation and cell proliferation, pathways closely associated with EMT...
August 28, 2018: Pathology, Research and Practice
Yuanyuan Kang, Jie Liu, Ying Zhang, Yan Sun, Junting Wang, Biying Huang, Ming Zhong
Ameloblastoma(AB) is an aggressive and slow-growing tumor with high recurrence rate, which arises from odontogenic epithelium. AB mostly shows osteolytic growth, but the specific pathogenesis is not yet clear. Periostin is a considered a prominent oncogene, which was mainly produced by osteoblasts and their precursors cells, it have been proved that Periostin play an important role in bone lysis. However, the precise role of Periostin in AB progression remains unknown. In this article, the surgical specimens from cases of AB were collected, and the Periostin expression was tested and the results were analyzed for possible correlations with clinical characteristics...
August 27, 2018: Pathology, Research and Practice
Y Uchihara, E Iwata, I Papadimitriou-Olivgeri, D Herrero-Charrington, Y Tanaka, N A Athanasou
BACKGROUND: Localised (transthyretin-associated) amyloid is commonly seen in articular/periarticular tissues of elderly individuals. Whether age-associated, amyloid deposition occurs in foot and ankle (F&A) tissues has not previously been investigated. In this study we assessed the nature and frequency of F&A amyloid deposition and determined whether it is associated with age and/or specific articular/periarticular F&A lesions. METHODS: Histological sections of twenty five normal F&A articular/periarticular tissues (16-71 years) and a range of F&A lesions were stained by Congo Red...
August 27, 2018: Pathology, Research and Practice
Naoki Wada, Satoshi Nojima, Shin-Ichiro Tahara, Kenji Ohshima, Masako Kurashige, Keisuke Kawasaki, Mitsuyo Tone, Shinya Kusumoto, Jun-Ichiro Ikeda, Eiichi Morii
Glutamine (Gln) is important not only for cell proliferation but also for differentiation. Although Gln is essential for plasmacytic differentiation of lymphocytes, no study has been done on the effect of Gln on differentiation of tumor cells, such as lymphoma. Here we examined the effect of Gln on plasmacytic differentiation of lymphoplasmacytic lymphoma (LPL) with its cell lines, MWCL-1 and RPCI-WM1. Gln promoted plasmacytic differentiation of LPL, and p38 MAPK signaling pathway mediated such differentiation...
August 27, 2018: Pathology, Research and Practice
Bi Tang, Ling Xuan, Mingming Tang, Hongju Wang, Jing Zhou, Jinjun Liu, Shili Wu, Miaonan Li, Xiaojing Wang, Heng Zhang
BACKGROUND: miR-93 is recently recognized to perform anti-inflammatory action in the pathological process of cardiomyocytes dysfunction. However, it remains unclear whether miR-93-3p involves in lipopolysaccharide (LPS)-induced inflammation and apoptosis in H9c2 cells. The present study aimed to investigate the functions of miR-93-3p and its target, toll-like receptor 4 (TLR4), in LPS-stimulated cardiomyocytes. MATERIAL AND METHODS: Cell viability was analyzed by CCK-8 assay...
August 24, 2018: Pathology, Research and Practice
Murtaza M Tambuwala, Prashant Kesharwani, Rahul Shukla, Paul D Thompson, Paul A McCarron
Fibrosis is known to be the hallmarks of chronic inflammation of the bowel. Epithelial damage due to inflammation compromises the barrier function of the gastrointestinal tract. This barrier dysfunction leads to further spread of inflammation resulting in a chronic state of inflammation. This chronic inflammation leads to development of fibrosis, which has very limited therapeutic options and usually requires surgical removal of the affected tissue. Our previous work has shown that Caffeic acid phenethyl ester (CAPE) is a naturally occurring anti-inflammatory agent, found in propolis, has been found to be protective in experimental colitis via enhancement of epithelial barrier function...
August 24, 2018: Pathology, Research and Practice
Xiuyan Wang, Ming Qi
BACKGROUND: MicroRNAs bind the 3' untranslated regions (3'-UTRs) of mRNAs and thereby regulate gene expression post-transcriptionally and play an important role in cancer delvelopment. In the present study, we have explored the role of miR-718 in papillary thyroid cancer cell malignancy. MATERIALS/METHODS: Here we examined the miRNA expression in human papillary thyroid cancer by RT-PCR. Luciferase activity, RT-PCR and western blot assays were used to confirmed the target of miRNA...
August 24, 2018: Pathology, Research and Practice
Daolin Ji, Xiangyu Zhong, Xingming Jiang, Kaiming Leng, Yi Xu, Zhenglong Li, Lining Huang, Jinglin Li, Yunfu Cui
OBJECTIVES: Long non-coding RNAs (lncRNAs) are a type Table of endogenous RNA longer than 200 nucleotides in length, and this kind of RNAs lack or possess limited ability of coding proteins. A large number of studies have demonstrated that lncRNAs could take part in massive biological processes, such as transcriptional activation and interference, cellular differentiation, proliferation, migration, invasion and apoptosis. The abnormal expression of lncRNAs has been clarified to play extremely important roles in various diseases, especially in human cancers...
August 20, 2018: Pathology, Research and Practice
Xinying Liu, Xiaodan Liu, Xiaoqiang Wang, Rui Wu, Kuangen Zhang, Beiying Liu, Qichen Liu, Yakun Shao, Ran Tang, Jiangfeng You, Fei Pei
Endometrial dedifferentiated carcinoma is a rare, malignant tumor whose molecular alterations have not been clarified yet. We report a novel case of a 61-year old woman who presented with irregular vaginal bleeding after menopause and a 3 cm uterus mass. Histology revealed endometrial dedifferentiated adenocarcinoma, a rare subtype comprised of undifferentiated adenocarcinoma. The patient still survived 1 year after surgery without chemotherapy and radiotherapy. Immunohistochemistry revealed loss of MLH1/PMS2 expression and retained MSH2/MSH6 expression...
August 20, 2018: Pathology, Research and Practice
Li Xue, Xiaolan Lu, Juntao He, Ting Zhang, Xiaokang Wu, Yanping Zhang, Ningning Wang, Zhe An, Jiru Xu, Yan Geng
BACKGROUND: CK 18-M30 was increased in patients with NAFLD. However, little is known about the relationship between CK 18-M30 and NAFLD progression. We aimed to analyze the variety of CK 18-M30 and other metabolism indices during NAFLD progression. Meanwhile, we aimed to investigate the correlation between CK 18-M30 and liver pathology during NAFLD progression. MATERIALS AND METHODS: Rats were fed with high sucrose and high fat diet for building NAFLD models. We detected liver pathology by hematoxylin-eosin (HE) staining...
August 20, 2018: Pathology, Research and Practice
Leandra Koletzko, Abdo Mahli, Claus Hellerbrand
Hepatic steatosis is common in patients infected with hepatitis C virus (HCV). Particularly in patients infected with non-genotype 3 HCV, hepatic steatosis is closely related to factors of the metabolic syndrome such as hyperlipidemia. However, the molecular mechanisms involved in this "metabolic" steatosis in non-3 genotype HCV infections are not well understood. Here, we aimed to develop an in vitro model to study the effect of genotype 1 HCV infection on hepatic lipotoxicity and lipid metabolism...
August 19, 2018: Pathology, Research and Practice
Jian-Zhu Yang, Xian-Da Wu, Jian-Bo Meng, Jin-Qiao Zhang, Li-Xia Sun
The aim of the study was to investigate whether microvessel density (MVD) could be associated with skeletal extramedullary disease relapse (skeletal-EMDR) in patients with multiple myeloma (MM) who have skeletal-EMD at diagnosis. Seventy-nine newly diagnosed MM patients who have skeletal-EMD were retrospectively enrolled in this study. The 4-year cumulative incidence of skeletal-EMDR was 35.0%±8.3%. The 4-year probability of overall survival (OS) was 54.0%±7.6%. Multivariate analysis showed that skeletal-EMDR (HR = 4...
August 19, 2018: Pathology, Research and Practice
Parnian Ahmadi Moghaddam, Rajesh Singh, Mandana Mahmoodi, Meenakshi Mehrotra, Gustavo Benaim, Rajyalakshmi Luthra, Alberto Paniz-Mondolfi
No abstract text is available yet for this article.
August 19, 2018: Pathology, Research and Practice
Qingting Bu, Hairong He, Di Fan, Jun Lyu, Zhenyu Pan, Haisheng You
BACKGROUND: Wilms' tumor (WT) is the most common pediatric renal tumor. Despite its high survival rate, the potential prognostic factors should further be studied to reduce the intensity of the treatment. A few studies have found LOH of 16q is associated with worse survival in patients with WT, but it is still contradictory. This study aimed to performed a meta-analysis to clarify this. METHODS: Databases including the Wanfang, PubMed, Chinese National Knowledge Infrastructure, Embase, and Cochrane Library databases were searched July 2018...
August 17, 2018: Pathology, Research and Practice
Chengxiang Zhang, Heng Qin, Rui Zheng, Yubang Wang, Ting Yan, Fei Huan, Yang Han, Weicheng Zhu, Lulu Zhang
This study was aimed to investigate whether ibuprofen could alter the P-glycoprotein expression and function under Alzheimer's Disease condition and whether this alteration was induced by the inhibition of inflammatory reaction. APP/PS1 mice were used as AD model mice and ibuprofen-treated AD mice were given ibuprofen for 5 months. Then, Abcb1a/1b mRNA levels and P-gp expression were evaluated by qRT-PCR and western blot. Abcb1 mRNA levels were significantly reduced in AD mice compared to control mice, and it could be restored by ibuprofen treatment...
August 17, 2018: Pathology, Research and Practice
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