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Pathology, Research and Practice

Lian-He Yang, Kimberly Ingersoll, Yue Zhao, Catherine Luedke, Siby Sebastian, Endi Wang
Aberrant expression of CD3 on diffuse large B-cell lymphoma (DLBCL) is rare, and its mechanism and biological significance are currently unclear. Herein we report a case of Epstein-Barr virus-negative, CD3-positive DLBCL in a 53 year-old male, who had a remote history of renal transplantation. After standard chemotherapy, the patient was in clinical remission. He relapsed three years later, but at this time with apparent loss of CD3 expression. PCR-based IGK gene rearrangement studies demonstrated clonal amplicons with an identical nucleotide size between the primary and secondary DLBCL, confirming the clonal relationship despite their phenotypic differences...
July 16, 2018: Pathology, Research and Practice
Kamuran Ibis, Sezer Saglam, Esra Kaytan Saglam, Pinar Firat, Dilek Yilmazbayhan, Alper Toker, Berker Ozkan, Veysel S Hancer, Murat Buyukdogan, Rian Disci, Kezban Nur Pilanci
BACKGROUND: To assess the prognostic importance of carbonic anhydrase IX (CA IX), a hypoxic biomarker, after neoadjuvant treatment in Stage III non-small cell lung cancer (NSCLC) patients. METHODS: Tissue CA IX expression was examined after surgical resection in 77 patients who had undergone neoadjuvant treatment. The effects of CA IX overexpression and other clinical factors on disease-free survival and overall survival were investigated. RESULTS: In multivariate analysis, number of neoadjuvant chemotherapy (CT) courses and gender emerged as significant independent predictors for disease-free survival, where administration of 2-3 courses of neoadjuvant chemotherapy (CT) (HR, 3...
July 11, 2018: Pathology, Research and Practice
Sujata Law, Shalini Sanyal, Ritam Chatterjee, Atrayo Law, Aditya Law, Sukalpa Chattopadhyay
RELEVANCE: Malignant peritoneal sarcomatosis related ascitic formation often leads to grave consequences but the therapeutic management of the fatal pathophysiological condition remains a rarely discussed issue. The present study investigates the anti-neoplastic activity of the plant alkaloid from Ruta graveolens on ascitic Sarcoma-180 bearing mice as a model of human malignant peritoneal ascites. MATERIALS AND METHODS: The efficacy of the loco-regional administration of Ruta graveolens on tumour cells was explored with cytopathological and cytotoxicological studies, along with the expressional modulation vital regulatory molecules viz...
July 11, 2018: Pathology, Research and Practice
Chun-Li Chen, Qing Ke, Ming Luo, Zi-Ye Gao, Zhi-Jiu Li, Zhi-Guo Luo, Dong-Bo Liu
As highly tissue-specific genes, it is increasingly recognized that long non-coding RNAs (lncRNAs) are considered as promising prognostic biomarkers for multiple human cancers. However, lack of tissue-specific lncRNAs in gastric cancer (GC) still exist. In this study, we identified a novel lncRNA LINC01939 which showed the largest fold change in GC than other human cancers from lnCAR database by bioinformatic analysis. Reverse transcription quantitative polymerase chain reaction (qPCR) assay confirmed that LINC01939 was significantly downregulated in GC tissues compared with matched normal tissues...
July 10, 2018: Pathology, Research and Practice
Rikke Karlin Jepsen, Louise Laurberg Klarskov, Michael Friis Lippert, Guy Wayne Novotny, Tine Plato Hansen, Ib Jarle Christensen, Estrid Høgdall, Lene Buhl Riis
Tumor budding is an independent prognostic factor in colorectal cancer. However, varying degrees of interobserver agreement and reproducibility challenges the use of tumor budding in diagnostics. Immunohistochemical staining of tumor slides with pan-cytokeratin visualizes the budding tumor cells and has been suggested to improve reproducibility. Here we demonstrate the methodology of tumor budding assessment using digital image analysis based on tumor slides stained for pan-cytokeratin, and investigate interobserver agreement, agreement between manual and digital assessment methods and digital reproducibility between users...
July 10, 2018: Pathology, Research and Practice
Zhongchuan Lv, Yong Zhang, Xiang Yu, Yang Lin, Yinlin Ge
Gastric cancer is one of the most common malignant tumors with the third mortality rate of cancer in world. A larger number of researches showed that genetic factors played an important role in the development of gastric cancer. More and more evidences have indicated that lncRNAs can regulate the gene expression through the transcription, post transcription and epigenetic levels including cancer invasion and metastasis, cell differentiation and apoptosis. However, the mechanisms of lncRNAs in the pathogenesis of gastric cancer remaining unclear...
July 7, 2018: Pathology, Research and Practice
Tae Jung Jang, Sun A Kim, Min Kyung Kim
Arginase 1 (Arg1) is involved in dampening the response of antitumor T lymphocytes. Arg1 expression has been reported in a variety of cancer cell lines and tumor-associated myeloid-derived cells. However, its examination in situ in tumor microenvironment is poorly investigated. We examined the Arg1-positive cells in tumor microenvironment of gastric carcinomas (GCs), colorectal carcinomas (CRCs) and prostate carcinomas (PCs), and analyzed their clinicopathological significance. Immunohistochemical staining for Arg1 was done in 60 GCs, 38 gastric adenomas, 40 CRCs, 10 colonic adenomas, 36 PCs, and 15 benign prostatic hyperplasia (BPH)...
June 28, 2018: Pathology, Research and Practice
Yoshitane Tsukamoto, Hiroyuki Futani, Takako Kihara, Takahiro Watanabe, Shunsuke Kumanishi, Shohei Matsuo, Seiichi Hirota, Takafumi Ueda, Hidetaka Yamamoto, Shinichi Yoshiya
We experienced a case of primary malignancy in giant cell tumor of bone (GCTB), arising in the right femur and harboring H3F3A mutation. A 27-year-old Japanese male without any prior disease history complained of pain in his right hip joint and right lower limb. Radiological images revealed an osteolytic and multicystic lesion existing mainly at the proximal epiphysis of the right femur. Preoperative clinical diagnosis was GCTB, although irregular marginal sclerosis was an atypical radiographic finding for conventional GCTBs...
June 28, 2018: Pathology, Research and Practice
Yukako Ishida, Akira Kido, Manabu Akahane, Shingo Kishi, Shinji Tsukamoto, Hiromasa Fujii, Kanya Honoki, Yasuhito Tanaka
This study aimed to clarify the role of mesenchymal stem cells (MSCs) as a component of the cancer microenvironment. We investigated the homing-related chemokine expression levels of MSCs treated with a prostate cancer cell line (PC-3) -conditioned medium. Among several homing chemokines, an antibody array revealed that expression of eotaxin-3 (but not eotxin-1 and -2) was highly enhanced in MSCs treated with PC-3-conditioned medium. A gene expression array showed significantly increased expression of CCR3, a receptor of eotaxin-3, in PC-3...
June 25, 2018: Pathology, Research and Practice
Milad Sabzevary-Ghahfarokhi, Mojtaba Shohan, Hedayatollah Shirzad, Ghorbanali Rahimian, Amin Soltani, Mahdi Ghatreh-Samani, Fatemeh Deris, Nader Bagheri, Mohammedhadi Shafigh, Kamran Tahmasbi
BACKGROUND: Reactive oxygen species (ROS) is one of the pathogenic factors responsible for intestinal injury in Ulcerative colitis (UC). Nuclear factor erythroid-2 related factor 2 (Nrf2) plays a critical role against ROS factors to conserve epithelial integrity. This study aimed to localize Nrf2 and IL-17A protein in the inflamed mucosa of patients with ulcerative colitis. The gene expression of Nrf2 was also correlated with GST-A4 and PRDX1. MATERIALS AND METHODS: A total of 20 patients and 20 healthy controls with definite UC based on the clinical criteria were enrolled for this study...
June 22, 2018: Pathology, Research and Practice
Nathália Martins Lopes, Flávia Caló Aquino Xavier, Rafael Carneiro Ortiz, Nádia Ghinelli Amôr, Gustavo Pompermaier Garlet, Vanessa Soares Lara, Aline Carvalho Batista, Nádia Lago Costa, Camila Oliveira Rodini
The biological process of epithelial-to-mesenchymal transition (EMT) has been studied in oral squamous cell carcinoma (OSCC) metastasis, but it is rarely evaluated at several stages of oral carcinogenesis. This study aimed to analyze the presence of SNAIL and E-cadherin proteins, markers of EMT, in the development and progression of OSCC, evaluating excised specimens of potentially malignant lesions (oral leukoplakia with and without dysplasia-OL and OLD, respectively), tumor tissues (OSCC), metastatic lymph nodes (LN), and normal oral mucosa (NOM) by immunohistochemistry, considering subcellular localization...
June 20, 2018: Pathology, Research and Practice
Fang Li, Li Ma, Cuizhi Geng, Chang Liu, Huiyan Deng, Meng Yue, Yan Ding, Xiaolin Wang, Yueping Liu
BACKGROUND: Accurate pathological assessment of breast specimens after NACT is crucial. It is beneficial to determine the treatment efficacy and predict prognosis. So we should explore the relevance between molecular subtypes and efficacy of neoadjuvant chemotherapy in breast cancer as well as its prognostic factors, which about survival analysis and disease free survival involved, which was one part of contributing for evaluating in terms of global survival and disease free survival...
June 19, 2018: Pathology, Research and Practice
Youn Jin Choi, Jungyoon Ho, Ahwon Lee, Soo Young Hur, Hyeon-Chun Park, Jong Sup Park, Yeun-Jun Chung, Sug Hyung Lee
Concurrence of both endometrial adenocarcinoma and ovarian adult granulosa cell tumor (aGCT) is believed to be related to high estrogen milieu, but genomic alterations of the concurrent endometrial adenocarcinoma and aGCT are not known. For this, we analyzed an uterine endometrial adenocarcinoma and an ovarian aGCT in a same patient by a targeted next generation sequencing (NGS). We found a germline mutation in STK11 (p.L113fs). The endometrial adenocarcinoma harbored FGFR2 and TP53 mutations and the aGCT harbored a FOXL2 (p...
June 19, 2018: Pathology, Research and Practice
Hans Bösmüller, Vanessa Pfefferle, Zeid Bittar, Veit Scheble, Marius Horger, Bence Sipos, Falko Fend
BACKGROUND: Microvessel density is an indicator of tumor-driven neoangiogenesis. Hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC) have distinct vascular patterns, which are also reflected in their imaging characteristics. Since a significant proportion of HCC are treated without biopsy confirmation, it is essential to discriminate HCC and ICC radiologically. The aim of our study was therefore to compare microvessel density and expression of VEGFR-2 in HCC and ICC, since these data may ultimately help us to better understand their imaging characteristics...
June 19, 2018: Pathology, Research and Practice
Huan-Yu Dai, Yan Ma, Zhao Da, Xiao-Ming Hou
OBJECTIVE: The tripartite motif 66(TRIM66) is an important member of the TRIM protein superfamily, which can participate in the expression of multiple proteins, and is closely associated with the behaviors of non-small cell lung cancer (NSCLC). In this study, we aimed to explore the effect of TRIM66 in this process in vitro using NSCLC cell lines, and the role of TRIM66 in regulating epithelial-mesenchymal transition(EMT) in NSCLC. METHODS: Western blotting was used to detect the TRIM66 protein expression levels in NSCLC cell lines and normal lung epithelial cells BEAS-2B...
June 18, 2018: Pathology, Research and Practice
John M Carney, Lin Wang, Rex Bentley, Diana M Cardona, Xuefeng Zhang
Squamous cell carcinoma with pseudoangiosarcomatous features is a rare but well-recognized variant of squamous cell carcinoma. These tumors exhibit complex anastomosing channels lined by neoplastic cells, histologically mimicking a vasoformative mesenchymal tumor. Immunohistochemically, the published cases expressed epithelial markers and were consistently negative for vascular markers. Squamous cell carcinoma with pseudoangiosarcomatous features and aberrant expression of vascular markers has never been reported...
June 12, 2018: Pathology, Research and Practice
Ruiqiong Ma, Zhijian Tang, Kunkun Sun, Xue Ye, Hongyan Cheng, Xiaohong Chang, Heng Cui
BACKGROUND: ADAM23, a member of the disintegrin and metalloprotease (ADAM) family, has been reported to be expressed in several types of tumours. Nevertheless, the exact role of ADAM23 in epithelial ovarian cancer (EOC) remains unclear. The aim of this study was to investigate ADAM23 expression in EOC and evaluate its clinicopathological and prognostic significance. METHODS: Immunohistochemistry (IHC), western blot and real-time PCR (RT-PCR) were used to analyse ADAM23 expression in 133 EOC, 42 benign ovarian tumour and 35 healthy control samples...
June 12, 2018: Pathology, Research and Practice
Xiangwen Zhang, Tingting Zhou, Wenbin Li, Taotao Zhang, Ninwei Che, Guo Zu
BACKGROUND: Few studies have reported the clinical and prognostic significance of C/EBP homologous protein (CHOP) in advanced gastric cancer (GC). Therefore, the present study investigated the expression of CHOP in advanced GC patients to determine its potential prognostic role. METHODS: The levels of CHOP in 95 patients with advanced GC and adjacent non-cancerous tissues were evaluated by qRT-PCR, western blot and immunohistochemistry. Furthermore, the association of CHOP expression with clinicopathological parameters and prognosis of advanced GC patients was analyzed...
June 12, 2018: Pathology, Research and Practice
José I López, Rafael Pulido, Jesús M Cortés, Javier C Angulo, Charles H Lawrie
Intratumor heterogeneity (ITH) detection remains a challenge in modern oncology because it can have a direct impact on the success of new therapies. Anti-PD-1/PD-L1 immunotherapy is an emerging treatment modality that is showing great promise for clear cell renal cell carcinoma (CCRCC) patients with advanced disease. Patient selection for such therapy relies upon the immunohistochemical detection of PD-1/PD-L1, however the degree of ITH for these markers among tumor cells and/or inflammatory mononuclear infiltrates remains unknown...
June 12, 2018: Pathology, Research and Practice
Xiaolin Peng, Yao Liu, Xin Peng, Zhengming Wang, Zhe Zhang, Yuling Qiu, Meihua Jin, Ran Wang, Dexin Kong
Olfactory neuroblastoma (ONB) is a kind of rare and complex head and neck tumor. The reports on this field are very scarce due to the low morbidity. Here, we summarized the clinical features and prognosis of ONB through analysis of 10 cases, and determined the phosphorylation status of some molecules known to be involved in carcinogesis such as Akt, Erk, Stat3 and Stat5 in ONB tissue. Ten ONB patients were recruited in this study, 6 male and 4 female, ranging from 26 to 66 years old. In the 10 cases, 6 were diagnosed as late T stage (T3 /T4 ), 6 were at late Kadish stage (C/D) and 3 were at high Hyams grade (Ⅲ), which indicated a poorer prognosis...
June 10, 2018: Pathology, Research and Practice
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