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Pathology, Research and Practice

Sonja Wörner, Hans-Konrad Mueller-Hermelink, Hans-Ullrich Voelker
In the present study, 21 cases of adult/late-onset EBV-associated lymphoproliferative disease (AELPD) with an uncertain malignant potential were investigated with regard to their histomorphology, immunophenotype, clonal rearrangement of the heavy chain (IgH) and T-cell receptor (TCR) genes and clinical course. The cases were histomorphologically reevaluated and assigned to one of three morphological groups: mononucleosis-like, Hodgkin-like, or polymorphous. In addition, cases with or without detectable necrosis were investigated for differences in clinical outcome...
December 16, 2017: Pathology, Research and Practice
David Emanuel Schwab, Guilherme Lepski, Christian Borchers, Katrin Trautmann, Frank Paulsen, Jens Schittenhelm
Immunohistochemistry is routinely used in differential diagnosis of tumours of the central nervous system (CNS). The latest 2016 WHO 2016 revision now includes molecular data such as IDH mutation and 1p/19q codeletion thus restructuring glioma classification. Direct comparative information between commonly used immunohistochemical markers for glial tumours GFAP, MAP - 2, NOGO - A, OLIG - 2 and WT - 1 concerning quality and quantity of expression and their relation to the new molecular markers are lacking. We therefore compared the immunohistochemical staining results of all five antibodies in 34 oligodendrogliomas, 106 ependymomas and 423 astrocytic tumours...
December 14, 2017: Pathology, Research and Practice
Jaroslaw Paluszczak, Katarzyna Kiwerska, Daniela Mielcarek-Kuchta
BACKGROUND: Aberrations in Wnt signaling pathway are related to the pathogenesis of head and neck carcinomas and their activation frequently results from epigenetic alterations. This study aimed to assess the frequency of the methylation of DAB2, which acts as a negative regulator of Wnt signaling, and correlate it with clinicopathological features in a group of oral cancer patients. MATERIAL AND METHODS: Forty nine patients with primary oral squamous cell carcinoma were enrolled in the study...
December 14, 2017: Pathology, Research and Practice
Eliane Macedo Sobrinho Santos, Rogério Gonçalves da Rocha, Hércules Otacílio Santos, Talita Antunes Guimarães, Carlos Alberto de Carvalho Fraga, Luiz Henrique da Silveira, Paulo Ricardo Batista, Paulo Sérgio Lopes de Oliveira, Geraldo Aclécio Melo, Sérgio Henrique Santos, Alfredo Maurício Batista de Paula, André Luiz Sena Guimarães, Lucyana Conceição Farias
Gallic acid is a polyphenolic compost appointed to interfere with neoplastic cells behavior. Evidence suggests an important role of leptin in carcinogenesis pathways, inducing a proliferative phenotype. We investigated the potential of gallic acid to modulate leptin-induced cell proliferation and migration of oral squamous cell carcinoma cell lines. The gallic acid effect on leptin secretion by oral squamous cell carcinoma cells, as well as the underlying molecular mechanisms, was also assessed. For this, we performed proliferation, migration, immunocytochemical and qPCR assays...
December 10, 2017: Pathology, Research and Practice
Rodolfo T C Takaoka, Nathália D Sertório, Lara P J Magalini, Leandro M Dos Santos, Helena R Souza, Melina M Iyomasa-Pilon, Lucas Possebon, Sara S Costa, Ana P Girol
The anti-inflammatory protein Annexin-A1 (ANXA1) is associated to tumor invasion process and its actions can be mediated by formylated peptides receptors (FPRs). Therefore, we evaluated the expression and correlation of ANXA1, FPR and cyclooxygenase-2 (COX-2) enzyme in esophageal and stomach inflammations and neoplasias. The study of proteins was performed by immunohistochemistry in biopsies of esophagitis, Barrett's esophagus, squamous cell carcinoma and adenocarcinoma of the esophagus, as well as gastritis, stomach polypus and gastric adenocarcinoma...
December 7, 2017: Pathology, Research and Practice
Ju Hwa Lee, Chang Hyeok An, Min Sung Kim, Nam Jin Yoo, Sug Hyung Lee
No abstract text is available yet for this article.
December 6, 2017: Pathology, Research and Practice
Felipe Ilelis, Nayra Soares do Amaral, Mariana Rezende Alves, Alexandre André Balieiro Anastácio da Costa, Vinícius Fernando Calsavara, Leonardo Lordello, Louise De Brot, Fernando Augusto Soares, Iara Sant'Ana Rodrigues, Rafael Malagoli Rocha
AIMS: High grade serous carcinoma (HGSC) is an aggressive tumour, and most patients relapse after treatment, acquiring resistance to platinum-based chemotherapy. One of the resistance mechanisms proposed is apoptosis evasion triggered by drug-related cytotoxic effect in the cell. In this context, this study aims to evaluate the protein expression of GRIM-19, NF-κB and IKK2, their association with chemotherapy response and to determine their prognostic values in HGSC. METHODS: GRIM-19, NF-κB and IKK2 expression was evaluated by immunohistochemistry (IHC) in 71 patients with HGSC selected between 2003 and 2013, whose underwent primary debulking surgery with complete cytoreduction...
December 6, 2017: Pathology, Research and Practice
C Laedrach, B Salhia, N Cihoric, I Zlobec, C Tapia
BACKGROUND: Tumor buds are associated with lympho-vascular invasion and lymph node metastases leading to the assumption that they are involved in the early metastatic process. Hence, it would be important to know if tumor buds can be targeted with the most widely used targeted therapies in breast cancer (BC) and if changes in hormone and Her2 status occur. The aim of this study was to answer these questions by determining whether hormone receptor (HR) and Her2 status are expressed in the tumor buds of a large cohort of BCs...
December 5, 2017: Pathology, Research and Practice
Ting-Juan Zhang, Hong Guo, Jing-Dong Zhou, Xi-Xi Li, Wei Zhang, Ji-Chun Ma, Xiang-Mei Wen, Xin-Yu Yao, Jiang Lin, Jun Qian
BACKGROUND: Accumulating studies have linked the disruptions of microRNA-10 (miR-10) to acute myeloid leukemia (AML) with NPM1 mutation. However, miR-10 expression and its clinical implication in AML remain poorly defined. Although a recent report showed high serum level of miR-10a was associated with adverse prognosis in AML, herein, we found bone marrow (BM) miR-10 overexpression was not a prognostic biomarker in AML. METHODS: BM miR-10 expression was examined by real-time quantitative PCR in BM mononuclear cells in 115 de novo AML patients and 45 controls...
December 5, 2017: Pathology, Research and Practice
Jiahui Zhang, Sainan Chen, Wenlie Chen, Yunmei Huang, Ruhui Lin, Meiya Huang, Yinsheng Wu, Liangpu Zheng, Zuanfang Li, Naishun Liao, Jinxia Ye, Xianxiang Liu
Osteoporotic osteoarthritis is a phenotype of osteoarthritis (OA) manifested as fragile and osteoporotic subchondral bone. However, the ultrastructural features of subchondral bone in osteoporosis OA have not been determined. The study was aimed to investigate the ultrastructural dynamic changes of subchondral bone in osteoporotic OA model and how the ultrastructural damage in the subchondral bone caused by osteoporosis deteriorated the cartilage damage in OA. Eighteen rabbits were equally randomized to three groups, including the control, the OA and the osteoporotic OA groups...
December 2, 2017: Pathology, Research and Practice
Hai-Biao Yan, Jia-Cheng Huang, You-Rong Chen, Jian-Ni Yao, Wei-Ning Cen, Jia-Yi Li, Yi-Fan Jiang, Gang Chen, Sheng-Hua Li
PURPOSE: To investigate the clinical value and potential molecular mechanisms of miR-1 in clear cell renal cell carcinoma (ccRCC). METHODS: We searched the Gene Expression Omnibus (GEO), ArrayExpress, several online publication databases and the Cancer Genome Atlas (TCGA). Continuous variable meta-analysis and diagnostic meta-analysis were conducted, both in Stata 14, to show the expression of miR-1 in ccRCC. Furthermore, we acquired the potential targets of miR-1 from datasets that transfected miR-1 into ccRCC cells, online prediction databases, differentially expressed genes from TCGA and literature...
December 2, 2017: Pathology, Research and Practice
Gabriela Molinari Roberto, Edgard Eduard Engel, Carlos Alberto Scrideli, Luiz Gonzaga Tone, María Sol Brassesco
Dysregulated mitotic kinases have frequently been associated with cancer. Changes in their expression might result from diverse mechanisms including avoidance of the tight regulation exerted by miRNAs. Herein we show that miR-10b* is downregulated in osteosarcoma samples and demonstrate its correlation with PLK1, PLK4, BUB1, and BUBR1, which are strongly intercorrelated. The selection of miRNAs that coordinately target and regulate multiple members of cancer-related pathways are particularly advantageous to tumors...
December 2, 2017: Pathology, Research and Practice
Hui Li, Jia Song, Guochao Niu, Hong Zhang, Jinbo Guo, David Q Shih, Stephan R Targan, Xiaolan Zhang
Tumor necrosis factor like cytokine 1A (TL1A) is a member of the TNF superfamily. Accumulating evidence demonstrated the importance of TL1A in the pathogenesis of inflammatory bowel disease (IBD) and suggested a potential role of TL1A blocking in IBD therapy. Here we aimed to explore whether the anti-TL1A antibody could ameliorate intestinal inflammation and fibrosis in IBD. A T cell transfer model of chronic colitis was induced by intraperitoneal injection of CD4+CD45RBhigh naive T cells isolated from either C57BL/6 wild type (WT) mice or LCK-CD2-Tl1a-GFP transgenic (L-Tg) mice into recombinase activating gene-1-deficient (RAG-/-) mice...
November 26, 2017: Pathology, Research and Practice
Man Zhu, Shili Qiu, Xianwei Zhang, Yingchao Wang, Tapara D M Souraka, Xue Wen, Chunzi Liang, Jiancheng Tu
PURPOSE: Published data have shown that vitamin D may have a protective effect on cancer development. CYP24A1, the main enzyme responsible for the degradation of active vitamin D, plays an important role in many cancer related cellular processes. Up to now, relationships between CYP24A1 polymorphisms and cancer susceptibility have been widely investigated, whereas the results are inconsistent. The aim of present meta-analysis was to explore the associations between CYP24A1 polymorphisms and cancer susceptibility...
November 21, 2017: Pathology, Research and Practice
Li Li, Qiong Yu, Weimin Liang
The middle cerebral artery occlusion (MCAO) model in rodents has been widely used as model for studying brain ischemic stroke. TTC (2,3,5-triphenyltetrazolium chloride) staining in fresh tissues is used to evaluate the size of the infarct in MCAO model, and TTC-stained brain tissues are considered to be possible to bring a damage to the anatomical structure of neuronal cells and unsuitable for immunofluorescence analyses of cytology, and discarded after evaluation of infarct volume. Another group of models with in vivo fixation was required to the pathological or histological analyses of the infarct brains, which lead to double the numbers of animals in researches...
November 21, 2017: Pathology, Research and Practice
Cristina Pérez-Ramírez, Marisa Cañadas-Garre, Ahmed Alnatsha, Eduardo Villar, Juan Ramón Delgado, Miguel Ángel Calleja-Hernández, María José Faus-Dáder
Lung cancer, particularly non-small cell lung cancer (NSCLC) subtype, is the leading cause of cancer-related death related worldwide. Numerous gene polymorphisms in DNA repair, folate and glutathione pathways have been associated with susceptibility of NSCLC. We conducted this study to evaluate the effects of ERCC1, ERCC2, ERCC5, XRCC1, XRCC3, MTHFR, MTR, MTHFD1, SLC19A1 and GSTP1 gene polymorphisms on risk of NSCLC. No association between these gene polymorphisms and susceptibility of NSCLC were found in our patients, suggesting that genetic variations in genes involved in DNA repair, folate and glutathione metabolism pathways may not influence the risk of NSCLC...
November 21, 2017: Pathology, Research and Practice
Wen-Jing Liu, Li Zhou, Zhi-Yong Liang, Wei-Xun Zhou, Lei You, Tai-Ping Zhang, Yu-Pei Zhao
BACKGROUND: It was found that G-protein-coupled receptor kinase 3 (GRK3) played key biological roles in some cancers. However, its associations with clinicopathologic features and prognosis in pancreatic ductal adenocarcinoma (PDAC) remain unknown. METHODS AND METHODS: Expression of GRK3 was detected, using tissue microarray-based immunohistochemistry, in paired formalin-fixed paraffin-embedded tumor and non-tumor samples from 165 patients with PDAC after curative resection, and was further correlated with clinicopathologic parameters and cancer-specific survival (CSS)...
November 20, 2017: Pathology, Research and Practice
Matthew Jeffreys, Susanne K Jeffus, Michael Herfs, Charles Matthew Quick
Evaluation of vulvar intraepithelial neoplasia (VIN) may be difficult due to overlapping histologic features seen in both usual (UVIN) and differentiated vulvar intraepithelial neoplasia (DVIN). DVIN represents a diagnostic challenge; poor inter-observer agreement is well documented. P53 has been described as a potentially helpful adjunct in some cases; however, intricacies in its interpretation remain. This study evaluated 41 consecutive cases which consisted of 23 keratinizing dysplasias that were morphologically suggestive of DVIN and 18 UVINs...
November 16, 2017: Pathology, Research and Practice
Jianghua Wu, Yanhui Zhang, Lin Sun, Qiongli Zhai
AIMS: Cyclin D1, generally considered to be absent in chronic lymphocytic leukaemia/small lymphocytic lymphoma (CLL/SLL), has been reported in the proliferation centres (PCs) of recent CLL/SLL cases. Cyclin D1 immunostaining in CLL/SLL may lead to diagnostic confusion. The objective of this study was to identify the types of stained cells and the impact on diagnosis. METHODS: Cyclin D1 expression was assessed by immunostaining samples from 46 cases of CLL/SLL. CD68 and double immunostaining with CD20/CyclinD1, CD68/CyclinD1, and CD163/CyclinD1 were then performed in cases of CLL/SLL positive for cyclinD1 in the PCs...
November 16, 2017: Pathology, Research and Practice
Ning Yu, TingGuo Zhang, DaHua Zhao, Zhang Cao, Jing Du, Qian Zhang
OBJECTIVE: Cancerous inhibitor of protein phosphatase 2A (CIP2A) is a recently identified oncoprotein that stabilizes c-Myc and promotes cell proliferation and transformation. Here, we investigated the clinical significance and biological function of CIP2A in endometrial cancer. METHOD: CIP2A expression was assessed in normal endometrium, endometrial hyperplasia, endometrial atypical hyperplasia, and endometrioid adenocarcinoma tissues using immunohistochemistry, western blot, and RT-PCR...
November 15, 2017: Pathology, Research and Practice
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