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Pathology, Research and Practice

Yoshitane Tsukamoto, Hiroyuki Futani, Shinichi Yoshiya, Takahiro Watanabe, Takako Kihara, Shohei Matsuo, Seiichi Hirota
We experienced a 38-year-old Japanese male with t(10;19) CIC-DUX4 -positive undifferentiated small round cell sarcoma in the deep abdominal wall. Three months before his first visit to our hospital, he noticed a mass in his right abdominal wall. Computed tomography on admission revealed a solid abdominal tumor 70×53mm in size and multiple small tumors in both lungs. The biopsy of the abdominal tumor revealed undifferentiated small round cell sarcoma, suggestive of Ewing sarcoma. Under the clinical diagnosis of Ewing-like sarcoma of the abdominal wall with multiple lung metastases, several cycles of ICE (ifosfamide, carboplatin and etoposide) therapy were performed...
June 6, 2017: Pathology, Research and Practice
Ho Jung An, Heejoon Jo, Chan Kwon Jung, Jin Hyoung Kang, Min Sik Kim, Dong-Il Sun, Kwang Jae Cho, Jung-Hae Cho, Hye Sung Won, Der Sheng Sun, Yoon Ho Ko
BACKGROUND: Excision repair cross complement group 1 (ERCC1) expression is a predictive biomarker for platinum-containing treatment in squamous cell carcinoma of head and neck (SCCHN). However, the prognostic significance after surgical resection is not well understood. METHODS: Oropharynx (n=143) or oral cavity (n=61) SCCHN patients undergoing surgery were included. ERCC1 protein expression and HPV status were assessed by ERCC1 and p16 immunohistochemistry. RESULTS: The ERCC1, over-expressed in 66...
May 31, 2017: Pathology, Research and Practice
Ranjie Yu, Chengyi Li, Xiaomei Lin, Qun Chen, Jie Li, Li Song, Lin Lin, Jingnan Liu, Yan Zhang, Wencui Kong, Xuenong Ouyang, Xiong Chen
MYBL2 (B-MYB), a member of the MYB family of transcription factor genes, regulates the expression of genes in the process of tumorigenesis. Many studies have shown that MYBL2 is high expresssion in several human malignancies including pancreatic ductal adenocarcinoma (PDAC). However, its role in PDAC is still unclear. The present study is designed to investigate MYBL2 expression levels and prognostic significance in PDAC patients. We assessed MYBL2 expression level by immunohistochemistry in tumor tissues from 93 PDAC patients undergoing curative resection...
May 4, 2017: Pathology, Research and Practice
Laura Lorenzon, Luana Ricca, Emanuela Pilozzi, Antoinette Lemoine, Valentina Riggio, Maria Teresa Giudice, Giuseppe Mallel, Flavio Fochetti, Genoveffa Balducci
INTRODUCTION: Recently TRG, necrosis grade and the rate of viable cancer cells of colorectal liver metastases were correlated with the response to chemotherapy treatments, whereas K-RAS mutations and c-MET over-expression were correlated with the prognosis. METHODS: 58 resection specimens were assessed for regression grades. Patients undergone neo-adjuvant treatments were compared to patients who underwent therapy exclusively adjuvantly. We investigated the K-RAS mutational profile, the c-MET over-expression along with patients' survivals curves...
April 21, 2017: Pathology, Research and Practice
Yeting Zeng, Xinrui Wang, Feilai Xie, Zhiyong Zheng
Ischemic pseudo-cellular crescent (IPCC) that is induced by ischemia and composed of hyperplastic glomerular parietal epithelial cells resembles cellular crescent. In this study, we aimed to assess the clinical and pathological features of IPCC in renal biopsy to avoid over-diagnosis and to determine the diagnostic basis. 4 IPCC cases diagnosed over a 4-year period (2012-2015) were evaluated for the study. Meanwhile, 5 cases of ANCA-associated glomerulonephritis and 5 cases of lupus nephritis (LN) were selected as control...
April 20, 2017: Pathology, Research and Practice
Hongmei Wu, Fen Zhang, Yu Chen, Chao Liu, Su Yao, Xiaolan Zhu, Xinlan Luo, Hengguo Zhuang, Yanhui Liu
Myxoid adrenocortical adenomas are uncommon. There were only 61 cases reports documented, and the tumors are tended to be misdiagnosed in virtue of being rare and distinctive histological features. Recently we encountered two myxoid adrenocortical adenoma cases of a 31-year-old Chinese woman and a 45-year-old Chinese man. The patients did not receive further treatment after surgery and were still alive after following up for 20 months. Myxoid adrenocortical adenomas is extremely rare. Recognition of this entity would be beneficial for pathologists to avoid msidiagnosis, and unnecessary treatment...
April 20, 2017: Pathology, Research and Practice
Lígia Lavezo Ferreira, Éder Ricardo Biasoli, Daniel Galera Bernabé, Cáris Maroni Nunes, Glauco Issamu Miyahara
BACKGROUND: Oral leukoplakia is considered a potentially malignant lesion for the development of squamous cell carcinoma, and various risk factors may be related to its development and malignant transformation, including the human papillomavirus (HPV). The aim of this case-control study was to detect the presence of HPV in fresh tissue, plasma and saliva samples obtained from patients with and without oral leukoplakia, and verify the correlation of the presence of DNA of HPV between different sources of materials...
April 20, 2017: Pathology, Research and Practice
Marcela Beghini, Márcia Fernandes de Araújo, Viviane Oliveira Severino, Renata Margarida Etchebehere, Denise Bertulucci Rocha Rodrigues, Sanívia Aparecida de Lima Pereira
OBJECTIVE AND DESIGN: The aim of the present study was to evaluate the immunohistochemical expression of Gal-1, Gal-3 and Gal-9 in the colon of chronic chagasic patients compared to biopsied non-chagasic patients. MATERIAL OR SUBJECTS: Thirty-two colon fragments were selected from chagasic patients with megacolon (n=25) and nonchagasic patients without megacolon (n=7). METHODS: Immunohistochemistry for Gal-1, Gal-3 and Gal-9 was performed using a common light microscope and the results were scored 0-3 according to labeling intensity...
April 20, 2017: Pathology, Research and Practice
Yuqi Luo, Haitao Yu, Wentao Ou, Lin Jia, Yaoxing Huang
OBJECTIVE: To investigate the characterization of intestinal epithelial stem cells stained by Rhodamine 123 (Rho) and analyze the dynamic changes of intestinal epithelial stem cells during the injured-repaired progress induced by 5-FU. METHODS: Mucosal cells were obtained from adult C57BL/6J mice. The Rho stained cells were sorted using FACS. The mouse model of intestinal mucosal injured-repaired was established by injecting 5-FU and sacrificed at different time post-injection, and the middle intestines were used for detecting the percentage of Rho low staining cell fraction by FACS and detecting the expression of the intestinal epithelial stem cells marker-musashi-1 (msi-1) by RT-PCR and immunohistochemistry...
April 20, 2017: Pathology, Research and Practice
Naoki Wada, Jun-Ichiro Ikeda, Hiromasa Tanaka, Hajime Sakakita, Masaru Hori, Yuzuru Ikehara, Eiichi Morii
Nonequilibrium atmospheric pressure plasma (NEAPP) is a novel approach for blood coagulation, wound healing, and tumor elimination. NEAPP not only directly but also indirectly affects living cells via the medium exposed to NEAPP-yielding devises, called plasma-activated medium (PAM). The conservable and portable PAM serves as an alternative and advantageous approach over direct NEAPP. Here we examined the effect of PAM on lymphoplasmacytic lymphoma (LPL) cell lines. We found that PAM induced plasma cell differentiation and reduced tumorigenic population...
April 20, 2017: Pathology, Research and Practice
Haifeng Li, Yanyang Zhang, Yuhang Pan, Dayang Hui, Jianning Chen, Yi Jin
Hepatic embryonal rhabdomyosarcoma (ERMS) is extremely rare. Here we report the simultaneous occurrence of hepatocellular carcinoma (HCC) and ERMS of the liver in a 40-year-old man without any symptoms. Macroscopically, the mass was composed of two different tumors. The large tumor was 4.5×4×4cm and was poorly circumscribed and soft in the central region of left lateral lobe of the liver with apparently focal necrosis. The small tumor, with diameter of 1cm, was adjacent to the large tumor without clear boundary...
April 20, 2017: Pathology, Research and Practice
Nae Yu Kim, Jung-Soo Pyo
OBJECTIVE: The aim of this study was to investigate the clinicopathological characteristics and prognostic role of enhancer of zeste homologue 2 (EZH2) expression in non-small cell lung cancer (NSCLC). METHODS: The correlation between EZH2 expression and the clinicopathological characteristics, including sex, smoking history, tumor differentiation, histologic type, tumor stage, and lymph node metastasis, was evaluated through a meta-analysis. In addition, the prognostic value of EZH2 expression was elucidated...
April 20, 2017: Pathology, Research and Practice
Bing Wei, Pengfei Ren, Chengjuan Zhang, Zhizhong Wang, Bing Dong, Ke Yang, Jiuzhou Zhao, Shichun Tu, Jie Ma, Yongjun Guo
Lung cancer with EGFR mutation is often associated pathological characteristics and good responses to EGFR tyrosine kinase inhibitors (TKIs). However, certain types of rare EGFR mutations have be linked to cases with poor response to EGFR TKIs. Therefore, extensive molecular screening and pathological characterization are essential for accurate diagnosis and selection of effective treatment plans. Although a large body of studies have established the rate of EGFR mutations as a whole entity, the rates of each individual types of mutations, especially those rare ones, have not been precisely determined in large patient populations with uniform genetic background...
April 20, 2017: Pathology, Research and Practice
Yuichi Koyama, Teppei Morikawa, Jimpei Miyakawa, Yu Miyama, Tohru Nakagawa, Yukio Homma, Masashi Fukayama
Diagnosis of upper urinary tract urothelial carcinoma in ureteroscopic biopsies is challenging. Therefore, an immunohistochemical marker that can differentiate between malignant and benign urothelium and predict final pathological features is necessary. In this study, we investigated Ki-67 expression in 26 ureteroscopic biopsies of the ureter and renal pelvis diagnosed with urothelial carcinoma (UC) and in 13 biopsies with non-neoplastic urothelium, using digital image analysis. The median Ki-67 labeling index was 1...
April 20, 2017: Pathology, Research and Practice
Yang Wang, Xin-Li Kang, Fan-Chang Zeng, Cong-Jie Xu, Jia-Quan Zhou, Dong-Ni Luo
OBJECTIVE: The study is performed to explore the correlations of forkhead box O3 (FoxO3) and forkhead box O4 (FoxO4) expressions with clinicopathological features and prognosis of bladder cancer. METHODS: Bladder cancer tissues and adjacent normal tissues from the recruited 222 patients were collected. Quantitative real-time polymerase chain reaction (qRT-PCR), Western blotting and immunohistochemistry were applied to determine the expressions of FoxO3 and FoxO4...
April 20, 2017: Pathology, Research and Practice
Jun Li, Ya-Fen Yu, Chang-Hua Liu, Cui-Mei Wang
OBJECTIVES: CD163 and CD206, markers of M2 macrophages, possesses anti-inflammatory properties. This study aims to investigate the clinicopathologic significance of M2 macrophages in patients of glomerulonephritis with crescents. METHODS: Renal tissue samples from patients of glomerulonephritis with more than 30% cell or cell-fibrous crescents, including lupus nephritis (LN, n=14), anti-neutrophil cytoplasmic antibody-associated vasculitis (AAV, n=14), IgA nephropathy(IgAN) (n=11), Henoch Schonlein purpura glomerulonephritis(HSPGN)(n=8)were included in this study...
April 20, 2017: Pathology, Research and Practice
Jing Chen, Junxia Zhao, Xiaofen Zhou, Shuang Liu, Yongxin Yan, Yanling Wang, Cuili Cao, Shou Han, Najing Zhou, Yannan Xu, Juan Zhao, Yunli Yan, Huixian Cui
Topoisomerase IIβ (topoIIβ) is a nuclear enzyme specifically expressed in neurons, and plays an important role in the development of the cerebellum. To date, the expression of topoIIβ protein in medulloblastoma (MB) has not been investigated. In this study, 16 MB specimens including 10 classical subtypes of MB and 6 desmoplastic subtypes of MB (DMB), along with 5 normal cerebellum samples, were obtained from clinics. With immunohistochemical staining, prominently expressed topoIIβ was seen in normal cerebellar tissues, while there was no or less pronounced staining in classical MB cells...
April 20, 2017: Pathology, Research and Practice
Yi Wang, Huijun Hu, Qiong Wang, Zhongwu Li, Yumin Zhu, Wei Zhang, Yanling Wang, Hongbing Jiang, Jie Cheng
Accumulating evidence has revealed that aberrant abundance of 5-hydroxymethylcytosine (5hmC) is critically involved in tumorigenesis. The aim of the present study was to investigate the level of 5hmC in primary oral squamous cell carcinoma (OSCC) and determine its clinical significance as well as prognostic value in predicting patients' outcomes. The expression levels of 5hmC in 95 human OSCC samples and 24 normal oral mucosa were evaluated by immunohistochemical staining. Moreover, the associations between the expression status of 5hmC and several clinicopathological parameters as well as patients' survival were further statistically assessed...
April 19, 2017: Pathology, Research and Practice
Burcu Saka, Ozgur Ekinci, Ayse Dursun, Nalan Akyurek
OBJECTIVE: To investigate the immunohistochemical expressions of HIF-1α, CA9 and CXCR4 in resected human CRC specimens in relation to clinicopathologic and prognostic variables. METHODS: A total of 186 patients (mean(SD) age: 56.7(12.6) years, 54.0% were males) with colorectal adenocarcinoma were included in this retrospective study. Resection specimens of the primary tumor were reviewed to confirm the diagnoses and the stage of the disease. Data on age, gender, tumor characteristics (localization, size, macroscopic growth pattern, histologic type, grade, angiolymphatic invasion, TNM stage), applied treatments and clinical outcome (overall survival, local recurrence and distant metastasis) were obtained from the hospital records...
April 14, 2017: Pathology, Research and Practice
Xuchen Zhang, Hongfa Zhu, Xiu Yang, Volkan N Adsay, Dhanpat Jain
Pancreatic "acinar cell cystadenoma" (PACA) is a rare benign pancreatic cystic lesion showing acinar cell differentiation. The neoplastic nature of PACA has been questioned and its exact pathogenesis remains unclear. To investigate that acinar cell differentiation is a non-specific metaplastic phenomenon that can occur in pancreatic ductal system, especially when chronically inflamed and dilated, and doesn't necessarily imply an acinar cell neoplasm, we retrospectively analyzed cases diagnosed as PACA and cases with post-obstructive cystic dilatation of pancreatic ducts for acinar cell differentiation using immunohistochemistry for trypsin...
April 1, 2017: Pathology, Research and Practice
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