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Pathology, Research and Practice

Chen Wang, Jing Luo, Jialing Rong, Siying He, Lei Zhang, Fang Zheng
BACKGROUND: The S100 protein family is implicated in tumor invasion and metastasis, but its prognostic roles in gastric cancer (GC) has not been elucidated. MATERIALS AND METHODS: In the current study, Kaplan-Meier plotter (KM plotter) database integrated the expression data and survival information of 1065 GC patients were downloaded from the Gene Expression Omnibus (GEO) (GSE22377, GSE14210 and GSE51105) that published by the three major cancer centers (Berlin, Bethesda and Melbourne)...
November 2, 2018: Pathology, Research and Practice
Lorenzo Alibardi
The ultrastructural immunolocalization of telomerase and hyaluronate has been studied in a case of oropharyngeal squamous carcinoma. Immunofluorescence shows that telomerase immunolabeling is present in the cytoplasm and in nuclei of some keratinocytes during their migration into the underlying connective tissue. The electron microscope shows that the nuclear localization of telomerase mainly occurs in the large nucleoli and in likely Cajal bodies, the sites of assembling and maturation of proteins forming the telomerase complex...
November 2, 2018: Pathology, Research and Practice
Patricia J Brooks, Jeffrey W Chadwick, Marco Caminiti, Brendan Dickson, Iona Leong
Primary aneurysmal bone cyst (ABC) is a cystic bone neoplasm characterized by disease-defining gene fusions involving the USP6/Tre2 gene. The literature describing gnathic ABC is limited. This case report describes a 27-year-old man presenting with a long-standing left-sided facial asymmetry. Multi-detector computed tomography imaging demonstrated a large expansile lesion positioned within the left condylar head. The lesion was biopsied and resected. The specimen showed a giant cell-rich cystic neoplasm, with fibrous tissue lined by multinucleated giant cells...
November 2, 2018: Pathology, Research and Practice
Zane Vitenberga, Māra Pilmane, Aurika Babjoniševa
INTRODUCTION: Chronic obstructive pulmonary disease (COPD) is a progressive chronic disease leading to obstructive lung airways and airflow limitations. The background of COPD is extensive cytopathology and histopathology orchestrated by mostly chronic inflammation with the local release of inflammatory, anti-inflammatory and regulatory mediators, as well as further remodeling and shaping of local architecture. Inflammatory mechanisms are provided by complex intercellular signalling networks and regulation of locally occurring immune responses...
October 29, 2018: Pathology, Research and Practice
Yunfeng Jin, Wenjie Zheng, Li Li, Guoqin Huang, Ya Liu, Haiyan Jiang, Yuexiang Zhang, Chunhui Tang
INTRODUCTION: The expression of bone morphogenetic protein-10 (BMP-10) is downregulated in some cancer types, but its function and mechanism in ovarian cancer remains unclear. MATERIALS AND METHODS: BMP-10 expression was detected in ovarian cancer tissues and cell lines by using immunochemistry and western blotting. Prognostic value of BMP-10 was evaluated by Kaplan-Meier curve and Cox regression model. Knockdown or overexpression of BMP-10 was conducted by using specific siRNA or pcDNA-BMP-10 in ovarian cancer cell lines...
October 28, 2018: Pathology, Research and Practice
Fangchao Mei, Jia Yu, Man Li, Mingwei Xiang, Yupu Hong, Yu Zhou, Yundong You, He Xia, Hongzhong Jin, Weixing Wang
OBJECTIVE: For patients with acute necrotizing pancreatitis (ANP), a high body mass index (BMI) increases the likelihood of acute hepatic injury (AHI). In the current study, we explored whether magnesium isoglycyrrhizinate (MgIg) could alleviate ANP-induced liver injury in obese rats. METHODS: Sprague-Dawley (SD) rats were selected for the present study, and the ANP model was established by retrograde injection of 5% sodium taurocholate into the biliary-pancreatic duct...
October 28, 2018: Pathology, Research and Practice
Miguel Barquín, Constanza Maximiano, Clara Pérez-Barrios, Estela Sanchez-Herrero, María Soriano, Marta Colmena, Manuel García-Espantaleón, Eva Tejerina González, Lourdes Gutierrez, Antonio Carlos Sánchez Ruiz, María Torrente, Mariano Provencio, Atocha Romero
Genetic screening for BRCA mutations should be offered to all women diagnosed with epithelial ovarian, fallopian tube, and/or peritoneal cancers given the implications for treatment options and cancer risk assessments. Yet, while germline breast cancer susceptibility gene 1 (BRCA1) and breast cancer susceptibility gene 2 (BRCA2) testing is commonly performed, BRCA1/2 somatic mutations testing is rather challenging since the poor quality of DNA extracted from formalin fixed paraffin embedded (FFPE) samples can significantly impair this process...
October 28, 2018: Pathology, Research and Practice
Menglan Li, Qianyun Deng, Lei Zhang, Siying He, Jialing Rong, Fang Zheng
BACKGROUND: The lymphocyte to monocyte ratio (LMR), a novel systematic biomarker of inflammation, has been reported to be associated with the progression and prognosis of many malignant cancers. However, the relationship between LMR and survival outcome of urological cancers (UCs) remains controversial. Herein, we conducted a meta-analysis to identify the prognostic value of pretreatment LMR in patients with UCs. METHODS: A literature search was performed in PubMed, Web of Science, Embase, Cochrane Library, Cochrane Central Register of Controlled Trials, Scopus, and CINAHL databases up to July 2018...
October 24, 2018: Pathology, Research and Practice
Asterios Triantafyllou, Kenneth O Devaney, Jennifer L Hunt, Alessandra Rinaldo, Alfio Ferlito
Current knowledge of laryngeal neuroendocrine cells in man and other vertebrates is reviewed. Particular attention is paid to differences in the distribution of neuroendocrine cells between squamous and respiratory laryngeal mucosa, foetal versus post-natal spatial arrangements, relation to the laryngeal cavity and nerve fibres, and immunoreactivities of these cells. Methodological deficiencies and gaps in knowledge are outlined. Comparisons with neuroendocrine cells in lung and gut are drawn, caution with regard to existing histogenetic models of laryngeal neuroendocrine neoplasia is advised and lines of future research are suggested...
October 24, 2018: Pathology, Research and Practice
Shi-Qing Zhang, Wen-Kai Ni, Ming-Bing Xiao, Feng Jiang, Cui-Hua Lu, Rui-Hua Wang, Run-Zhou Ni
Apoptosis in intestinal epithelial cells (IECs) promotes the development of ulcerative colitis (UC), a type of inflammatory bowel disease (IBD). Efficient clearance of apoptotic cells is essential for tissue homeostasis in metazoans. Actin related protein 3 (ARP3) promotes endothelial dysfunction. The expression and function of ARP3 in UC remains unclear. In this study, the expression of apoptotic markers as p53, Bax, Cleaved-Caspease9 and Cleaved-Caspease3 were proved to be increased in the intestinal epithelial cells (IECs) of UC patients and in a mouse disuccinimidyl suberate(DSS)-induced colitis model; meanwhile, ARP3 expression was elevated...
October 24, 2018: Pathology, Research and Practice
Yanwen Xu, Renli Liu, Chuanpeng Liao, Jing Liu, Huafu Zhao, Zongyang Li, Wenlan Liu, Lei Chen, Changpeng Wu, Hui Tan, Zhongping Chen, Ni Xie, Weiping Li
Glioma is the commonest malignant tumor in the central nervous system (CNS), characterized by rapid growth. However, the molecular mechanism underlying the growth remains unclear. Immunity-related GTPase family M protein (IRGM) participates in immune response to pathogen and tumorigenesis. Proliferation and autophagy are two crucial functions contributing to aggressive growth. Therefore, our aims were to probe whether IRGM regulates glioma proliferation and autophagy. In this study, we found that 47 glioma specimens had more IRGM expression than 11 non-cancerous brain tissues with immunohistochemistry...
October 24, 2018: Pathology, Research and Practice
Chaoxi Zhou, Yong Li, Guiying Wang, Wenbo Niu, Jianfeng Zhang, Guanglin Wang, Qun Zhao, Liqiao Fan
Stomatin-like protein-2 (SLP-2) gene belongs to the stomatin supergene family, and previous studies have revealed up-regulated SLP-2 expression in gallbladder cancer, lung cancer, and esophageal cancer, while the role of SLP-2 in colorectal cancer (CRC) remains unclear and needs further investigation. Therefore, the expression levels of SLP-2 in CRC tissue and cell lines were tested in this study. Besides, we further explored the role of SLP-2 in CRC invasion and metastasis at molecular level via gene intervention technique...
October 24, 2018: Pathology, Research and Practice
Fa-Zheng Shen, Xiang-Sheng Li, Ji-Wei Ma, Xiang-Yang Wang, Shu-Peng Zhao, Lei Meng, Shu-Feng Liang, Xin-Li Zhao
BACKGROUND: Cell division cycle associated 7 like (CDCA7L) belongs to the JPO protein family, which is recently identified as a target gene of c-Myc and is frequently dysregulated in multiple cancers. This study aimed to explore the clinicpathological value and biological role of CDCA7L in glioma. METHODS: CDCA7L expression in glioma patients was determined using the Oncomine database, and the prognostic role of CDCA7L expression was assessed in a retrospective cohort study...
October 24, 2018: Pathology, Research and Practice
Simona Gurzu, Tivadar Bara, Calin Molnar, Tivadar Bara, Vlad Butiurca, Hanga Beres, Simin Savoji, Ioan Jung
BACKGROUND: Pancreatic mucinous cystic neoplasms (MCN) are rare tumors that are usually diagnosed in females. MATERIALS AND METHODS: In our department, only four of the 109 consecutive cases of pancreatic tumors (3.67%) were diagnosed as MCNs. In this report, we present the characteristics of these four specific cases which also showed unusual HER-2 positivity and neuroendocrine differentiation. RESULTS: The four MCNs were diagnosed in patients with ages between 46 and 75 years...
October 23, 2018: Pathology, Research and Practice
Li Jiang, Chang-Liang Wu, Ji-Zhou Wu, Xia Yang, Han-Lin Wang, Guo-Jian Li
The clinical role and potential molecular mechanisms of microRNA-449c-5p (miR-449c-5p) in hepatocellular carcinoma (HCC) tissues remains unclear. Combining multiple bioinformatic tools, we studied the miR-449c-5p expression levels in HCC tissues and explored possible target genes and related signaling pathways. First, miR-449c-5p expression data from microarrays provided by publicly available sources were mined and analyzed using various meta-analysis methods. Next, genes that were downregulated after miR-449c-5p mimic transfection into HCC cells were identified, and in silico methods were used to predict potential target genes...
October 23, 2018: Pathology, Research and Practice
Andrej Belančić, Luka Vranić, Ivan Ševeljević, Ita Hadžisejdić, Antica Duletić Načinović, Nives Jonjić
An association between autoimmune events, as well as the development of antiphospholipid (aPL) antibodies and lymphoproliferative disorders is well recognized. We present the patient with coagulation abnormalities and non-Hodgkin lymphoma (NHL), primarily diagnosed as nodal marginal zone B-cell lymphoma (NMZL), and in relapse as diffuse large B-cell lymphoma (DLBCL). In the follow-up period, the patient simultaneously developed different aPL antibodies. The presence of aPL antibodies in NHL is frequent but it is not common in the NMZL...
October 23, 2018: Pathology, Research and Practice
Yoon Jin Cha, Hyo Sup Shim, Joungho Han, Yong Soo Choi
BACKGROUND: We aimed to investigate the clinicopathologic and histopathologic characteristics of colloid adenocarcinoma of the lung. METHODS: We reviewed the data of 10 patients with colloid adenocarcinoma that met the definition and histopathologic criteria of the current classification of the World Health Organization. The clinicopathologic characteristics were analyzed. Comprehensive histologic review and immunohistochemical staining for TTF-1, ALK, CDX2, and MUC5AC as well as a molecular examination for EGFR and KRAS was performed...
October 23, 2018: Pathology, Research and Practice
Morten Ragn Jakobsen, Chinachote Teerapakpinyo, Shanop Shuangshoti, Somboon Keelawat
BACKGROUND: Assessment of HER2 status is considered standard of care in the histopathologic workup of breast cancer and conveys prognostic and predictive information used to guide treatment decisions. The assessment is often carried out in a two-step approach where immunohistochemical expression of HER2 protein is first evaluated by conventional microscopy and equivocal cases are further analyzed by in-situ hybridization techniques to assess gene amplification status. In this study we compared conventional manual assessment of immunohistochemical HER2 expression with digital image analysis (DIA) and consensus manual assessment by a panel of three pathologists...
October 23, 2018: Pathology, Research and Practice
Fernanda Rodrigues Helmo, Renata Margarida Etchebehere, Natália Bernardes, Maria Flávia Meirelles, Caetano Galvão Petrini, Laura Penna Rocha, Maria Luíza Gonçalves Dos Reis Monteiro, Camila Souza de Oliveira Guimarães, Vicente de Paula Antunes Teixeira, Marlene Antônia Dos Reis, Juliana Reis Machado, Rosana Rosa Miranda Corrêa
This literature review aims to address the main scientific findings on oxidative stress activity in different gestational disorders, as well as the function and application of melatonin in the treatment of fetal and neonatal changes. Oxidative stress has been associated with the etiopathogenesis of recurrent miscarriages, preeclampsia, intrauterine growth restriction, and stillbirth. Both, the exacerbated consumption of the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase, and the increased synthesis of reactive oxygen species, such as superoxide, peroxynitrite, and hydrogen peroxide, induce phospholipid peroxidation and endothelial dysfunction, impaired invasion and death of trophoblast cells, impaired decidualization, and remodeling of maternal spiral arteries...
October 23, 2018: Pathology, Research and Practice
Dajiang Wu, Hao Zhang, Fang Ji, Wenbin Ding
MicroRNAs (miRNAs) are abnormally expressed in numerous diseases, which are intimately associated with cell proliferation, migration and invasion. Recent study indicated that miR-17 may be involved in regulating osteosarcoma (OS) occurrence and development, but its function and mechanism have not been reported. In this study, quantitative real-time PCR (qRT-PCR) was used to measure the expression of miR-17, and Western blotting assay was performed to measure the expressions of SAM and SH3 domain containing 1 (SASH1), phosphoinoinositide-3 kinase (PI3K), protein kinase B (AKT), Caspase3, Bcl-2 gene family (Bcl-2, Bax) and matrix metalloprotein (MMP-2, MMP-9) in MG-63 cells...
October 22, 2018: Pathology, Research and Practice
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