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Plasmid

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https://www.readbyqxmd.com/read/28219792/conjugative-type-iv-secretion-in-gram-positive-pathogens-trag-a-lytic-transglycosylase-and-endopeptidase-interacts-with-translocation-channel-protein-tram
#1
Verena Kohler, Ines Probst, Andreas Aufschnaiter, Sabrina Büttner, Lisa Schaden, Gerald Rechberger, Günther Koraimann, Elisabeth Grohmann, Walter Keller
Conjugative transfer plays a major role in the transmission of antibiotic resistance in bacteria. pIP501 is a Gram-positive conjugative model plasmid with the broadest transfer host-range known so far and is frequently found in Enterococcus faecalis and Enterococcus faecium clinical isolates. The pIP501 type IV secretion system is encoded by 15 transfer genes. In this work, we focus on the VirB1-like protein TraG, a modular peptidoglycan metabolizing enzyme, and the VirB8-homolog TraM, a potential member of the translocation channel...
February 17, 2017: Plasmid
https://www.readbyqxmd.com/read/28189631/episomal-lentiviral-vectors-confer-erythropoietin-expression-in-dividing-cells
#2
Feng Chen, Xin Qi, Rong Zhang, Zong-Yong Wu, Cui-E Yan, Jia Li, Qiu-Ying Liu, Jun Qi
Lentiviral vectors are now widely considered as one of the most common gene delivery tools for dividing and non-dividing cells. However, insertional mutagenesis has been found in clinical trials with retroviral vectors, which poses a safety risk. The use of non-integrating lentiviral (NIL) vectors, which avoid integration, eliminates the insertional mutagenesis problem. These NIL vectors are unable to mediate stable gene delivery into dividing cells, which makes them of limited use in the clinical practice of gene therapy...
February 8, 2017: Plasmid
https://www.readbyqxmd.com/read/28137396/comparative-analysis-of-the-standard-pcr-based-replicon-typing-pbrt-with-the-commercial-pbrt-kit
#3
Elisa Carloni, Francesca Andreoni, Enrica Omiccioli, Laura Villa, Mauro Magnani, Alessandra Carattoli
Plasmids are the main vectors of resistance and virulence genes in Enterobacteriaceae and plasmid typing is essential for the analysis of evolution, epidemiology and spread of antibacterial resistance. The PCR-Based Replicon Typing (PBRT), developed by Carattoli et al. in 2005, was an efficient method for plasmid identification and typing in Enterobacteriaceae. The 2005 PBRT scheme detected 18 replicons in 8 PCR reactions. Recently, the identification of novel replicons and plasmid types requested an update of the PBRT scheme...
January 27, 2017: Plasmid
https://www.readbyqxmd.com/read/28130036/construction-of-a-biobrick%C3%A2-compatible-vector-system-for-rhodococcus
#4
James Ellinger, Claudia Schmidt-Dannert
Throughout the past decade, the field of synthetic biology has grown rapidly. By using assembly platforms such as BioBricks™, scientists can quickly and easily build gene circuits or multi-step pathways. One limitation, however, is that most of these parts were designed and characterized with Escherichia coli as the target chassis. As a consequence, there exists a lack of standardized and well characterized or BioBrick™ compatible plasmid backbones that replicate in other potential non-model chassis organisms...
January 24, 2017: Plasmid
https://www.readbyqxmd.com/read/28119062/baculovirus-based-genome-editing-in-primary-cells
#5
Maysam Mansouri, Zahra Ehsaei, Verdon Taylor, Philipp Berger
Genome editing in eukaryotes became easier in the last years with the development of nucleases that induce double strand breaks in DNA at user-defined sites. CRISPR/Cas9-based genome editing is currently one of the most powerful strategies. In the easiest case, a nuclease (e.g. Cas9) and a target defining guide RNA (gRNA) are transferred into a target cell. Non-homologous end joining (NHEJ) repair of the DNA break following Cas9 cleavage can lead to inactivation of the target gene. Specific repair or insertion of DNA with Homology Directed Repair (HDR) needs the simultaneous delivery of a repair template...
January 22, 2017: Plasmid
https://www.readbyqxmd.com/read/28109681/editorial
#6
EDITORIAL
Julian I Rood, Christopher M Thomas, Eva M Top
No abstract text is available yet for this article.
January 18, 2017: Plasmid
https://www.readbyqxmd.com/read/28063893/a-plasmid-encoded-dsba-homologue-is-a-growth-phase-regulated-thioredoxin
#7
Amada Díaz-Magaña, Martha P Chávez-Moctezuma, Jesús Campos-García, Martha I Ramírez-Díaz, Carlos Cervantes
The Pseudomonas aeruginosa plasmid pUM505 contains in a pathogenicity island the dsbA2 gene, which encodes a product with similarity to DsbA protein disulfide isomerases, enzymes that catalyze formation and isomerization of disulfide bonds in protein cysteine residues. Using transcriptional fusions, it was found that dsbA2 gene promoter is activated during the stationary phase, suggesting that DsbA2 protein may be required for adaptive changes that occur during this stage of bacterial growth. Transfer of the pUM505 dsbA2 gene to a cadmium-sensitive P...
January 4, 2017: Plasmid
https://www.readbyqxmd.com/read/28034789/bibac-gw-based-vectors-for-generating-reporter-lines-for-site-specific-genome-editing-in-planta
#8
Damar Tri Anggoro, Mariliis Tark-Dame, Aimee Walmsley, Rurika Oka, Mara de Sain, Maike Stam
When generating transgenic plants, one of the objectives is to achieve stable expression of the transgene. Transgene silencing can be avoided by single copy integration of the transgene. Binary systems that predominantly result in single copy integrations, such as BIBAC vectors, are also single-copy in E. coli, the organism in which the T-DNA to be delivered to the plant is assembled. Although a low-copy number is important for stable maintenance of large DNA fragments in E. coli, it hampers cloning into the vector due to a low DNA yield...
December 26, 2016: Plasmid
https://www.readbyqxmd.com/read/27989736/construction-of-pduo-a-bicistronic-shuttle-vector-series-for-dual-expression-of-recombinant-proteins
#9
Paul A Nakata
Our ability to genetically manipulate microbial systems is often hampered by the availability of genetic tools. Thus, there is a need for the continued expansion of our molecular tool box. In support of this expansion, this study reports the design, construction, and validation of a new bicistronic shuttle vector series, pDUO, for the dual expression of genes in different hosts. Each vector was designed and constructed to contain two araC-pBAD inducible promoter systems for tight control over gene expression...
December 16, 2016: Plasmid
https://www.readbyqxmd.com/read/27916622/a-novel-group-of-incq1-plasmids-conferring-multidrug-resistance
#10
M Oliva, R Monno, P D'Addabbo, G Pesole, A M Dionisi, M Scrascia, M Chiara, D S Horner, C Manzari, I Luzzi, C Calia, A M D'Erchia, C Pazzani
The IncQ is a group of non-conjugative but mobilisable plasmids that are found and stably maintained in a wide range of bacteria contributing to the spread of antimicrobial resistance genes and to the insurgence of multidrug resistant bacteria. Here we report the identification, in clinical Salmonella Typhimurium strains, of an IncQ1 plasmid (pNUC) which confers resistance to sulfamethoxazole, streptomycin and tetracycline through the presence of sul2, strAB and tetA genes, respectively. pNUC was detected in five multidrug resistant S...
January 2017: Plasmid
https://www.readbyqxmd.com/read/27825973/an-efficient-system-for-the-generation-of-marked-genetic-mutants-in-members-of-the-genus-burkholderia
#11
Sravanthi Shastri, Helena L Spiewak, Aderonke Sofoluwe, Vigdis A Eidsvaag, Atif H Asghar, Tyrone Pereira, Edward H Bull, Aaron T Butt, Mark S Thomas
To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mutant allele to the genome of the host organism in place of the wild-type gene. However, due to their intrinsic resistance to many antibiotics only selected antibiotic-resistance markers can be used in members of the genus Burkholderia, including the Burkholderia cepacia complex (Bcc)...
January 2017: Plasmid
https://www.readbyqxmd.com/read/27890562/tailor-made-fibroblast-specific-and-antibiotic-free-interleukin-12-plasmid-for-gene-electrotransfer-mediated-cancer-immunotherapy
#12
Urska Kamensek, Natasa Tesic, Gregor Sersa, Spela Kos, Maja Cemazar
Electrotransfer mediated delivery of interleukin-12 (IL-12) gene, encoded on a plasmid vector, has already been demonstrated to have a potent antitumor efficacy and great potential for clinical application. In the present study, our aim was to construct an optimized IL-12-encoding plasmid that is safe from the regulatory point of view. In light of previous studies demonstrating that IL-12 should be released in a tumor localized manner for optimal efficacy, the strong ubiquitous promoter was replaced with a weak endogenous promoter of the collagen 2 gene, which is specific for fibroblasts...
November 24, 2016: Plasmid
https://www.readbyqxmd.com/read/27864039/effects-of-the-sequence-and-orientation-of-an-expression-cassette-in-tobacco-transformed-by-dual-bt-genes
#13
Tong Qiu, Yan Dong, Yachao Ren, Jinmao Wang, Minsheng Yang, Jun Zhang
This study investigated the effects of the sequence arrangement and orientation of a target gene expression cassette in vectors on expression levels to determine the optimal combination for highly efficient multi-gene expression. Five plant transformation vectors were constructed using dual Bt genes, Cry1Ac and Cry3A, which differed in the sequence arrangement and orientation of the target gene expression cassette. Through an Agrobacterium-mediated method, 5 vectors were used for the genetic transformation of tobacco to obtain transgenic lines...
November 15, 2016: Plasmid
https://www.readbyqxmd.com/read/27826018/analysis-of-pcerc7-a-small-antibiotic-resistance-plasmid-from-a-commensal-st131-escherichia-coli-defines-a-diverse-group-of-plasmids-that-include-various-segments-adjacent-to-a-multimer-resolution-site-and-encode-the-same-nika-relaxase-accessory-protein-enabling
#14
Robert A Moran, Ruth M Hall
The ampicillin resistance plasmid pCERC7, carrying transposon Tn2 with an IS4 insertion, was detected in the draft genome of a commensal Escherichia coli isolate. The genome data also revealed that this isolate belongs to ST131, clade B. pCERC7 is 9712bp comprised of a 3319bp backbone, Tn2::IS4 (6388bp) and 5bp of target site duplication, and was present at a copy number of 40. pCERC7 is related to several plasmids composed of only the backbone, or the backbone with the Tn2 insertion in the same position. These plasmids have been found previously in Escherichia coli or Salmonella enterica recovered in several different countries from as early as the 1970s...
November 5, 2016: Plasmid
https://www.readbyqxmd.com/read/27794439/corrigendum-to-construction-of-a-highly-active-liver-specific-transcriptional-regulatory-element-through-combination-of-the-albumin-promoter-and-%C3%AE-fetoprotein-enhancer-plasmid-65-2011-125-31
#15
E Q Chen, X Q Song, Y L Wang, T Y Zhou, L Bai, L Liu, C Liu, X Cheng, H Tang
No abstract text is available yet for this article.
October 26, 2016: Plasmid
https://www.readbyqxmd.com/read/27743797/enhanced-plasmid-loss-in-bacterial-populations-exposed-to-the-antimicrobial-compound-irgasan-delivered-from-interpenetrating-polymer-network-silicone-hydrogels
#16
Leise Riber, Mette Burmølle, Martin Alm, Stefan M Milani, Peter Thomsen, Lars H Hansen, Søren J Sørensen
The spread of antimicrobial resistance, usually mediated by horizontal transfer of plasmids, limits the options of treating bacterial infections and thereby poses a crucial human health problem. The disturbance of plasmid stability within bacterial species in clinical environments serves as a novel strategy to reduce the development and dissemination of antibiotic resistance. We tested the ability of irgasan to destabilize plasmids from Escherichia coli K-12 cells when added directly into liquid growth medium at concentrations below levels of marked bacterial growth inhibition, or when released into liquid growth medium from irgasan-impregnated Interpenetrating Polymer Network (IPN) silicone hydrogel objects, a novel technology developed as drug-delivery platform...
October 12, 2016: Plasmid
https://www.readbyqxmd.com/read/27693407/the-pultra-plasmid-series-a-robust-and-flexible-tool-for-fluorescent-labeling-of-enterobacteria
#17
Despoina A I Mavridou, Diego Gonzalez, Abigail Clements, Kevin R Foster
Fluorescent labeling has been an invaluable tool for the study of living organisms and bacterial species are no exception to this. Here we present and characterize the pUltra plasmids which express constitutively a fluorescent protein gene (GFP, RFP, YFP or CFP) from a strong synthetic promoter and are suitable for the fluorescent labeling of a broad range of Enterobacteria. The amount of expressed fluorophore from these genetic constructs is such, that the contours of the cells can be delineated on the basis of the fluorescent signal only...
September 29, 2016: Plasmid
https://www.readbyqxmd.com/read/27687731/conjugative-dna-transfer-in-streptomyces-a-mycelial-organism
#18
L Thoma, G Muth
Conjugative DNA-transfer in the Gram-positive mycelial soil bacterium Streptomyces, well known for the production of numerous antibiotics, is a unique process involving the transfer of a double-stranded DNA molecule. Apparently it does not depend on a type IV secretion system but resembles the segregation of chromosomes during bacterial cell division. A single plasmid-encoded protein, TraB, directs the transfer from the plasmid-carrying donor to the recipient. TraB is a FtsK-like DNA-translocase, which recognizes a specific plasmid sequence, clt, via interaction with specific 8-bp repeats...
September 28, 2016: Plasmid
https://www.readbyqxmd.com/read/27620651/destabilization-of-inca-and-incc-plasmids-by-sgi1-and-sgi2-type-salmonella-genomic-islands
#19
Christopher J Harmer, Mohammad Hamidian, Stephanie J Ambrose, Ruth M Hall
Both the Salmonella genomic islands (SGI) and the conjugative IncC plasmids are known to contribute substantially to the acquisition of resistance to multiple antibiotics, and plasmids in the A/C group are known to mobilize the Salmonella genomic island SGI1, which also carries multiple antibiotic resistance genes. Plasmid pRMH760 (IncC; A/C2) was shown to mobilize SGI1 variants SGI1-I, SGI1-F, SGI1-K and SGI2 from Salmonella enterica to Escherichia coli where it was integrated at the preferred location, at the end of the trmE (thdF) gene...
September 13, 2016: Plasmid
https://www.readbyqxmd.com/read/27615011/a-super-twin-t-dna-vector-that-allows-independent-gene-expression-during-agrobacterium-mediated-transformation
#20
Qiang Yang, Mei Deng, Ling-Ling Zhang, Xiao-Wei Zhang, Le-Ning Wang, Hu Chen, Jian Ma, Peng-Fei Qi, Qian-Tao Jiang, Xiu-Jin Lan, Yu-Ming Wei, You-Liang Zheng
In this study, we designed and constructed a super twin T-DNA vector (pTRIDT313-g) containing two independent T-DNA cassettes-one for the selection gene Hyg and the other for the target gene Gus-to produce marker-free transgenic lines. The resulting vector was transformed into tobacco, and polymerase chain reaction (PCR) analysis showed four types of gene combinations in the T1 and T2 generations: Gus only, Hyg only, Gus+Hyg, and untransformed lines. The intermediate region from the T-DNA of the right border of Hyg to the left border of Gus in the Hyg and Gus lines was not amplified...
September 8, 2016: Plasmid
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