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Developmental and Comparative Immunology

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https://www.readbyqxmd.com/read/28549733/divergent-roles-of-three-cytochrome-c-in-ctsb-modulating-coelomocyte-apoptosis-in-apostichopus-japonicus
#1
Huahui Chen, Miao Lv, Zhimeng Lv, Chenghua Li, Weiwei Zhang, Xuelin Zhao, Xuemei Duan, Chunhua Jin, Jinbo Xiong, Feng Xu, Ye Li
Cytochrome c plays crucial roles in apoptosis and the immune response. We previously demonstrated that cathepsin B from Apostichopus japonicus (AjCTSB) induces coelomocyte apoptosis. However, the mechanistic explanation and the regulation of this process have not been investigated. In the present study, we identified three cytochrome c cDNAs from A. japonicus (designated Ajcytc1, Ajcytc-1, and Ajcytc-2) using expressed sequence tag- (EST) and RACE-based approaches. The deduced amino acid sequences of the three cytochrome isoforms contained conserved CXXCH motifs, which are involved in binding heme and maintaining proteolytic activity...
May 23, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28545960/vaccination-with-multimeric-recombinant-vp28-induces-high-protection-against-white-spot-syndrome-virus-in-shrimp
#2
Suparat Taengchaiyaphum, Hideki Nakayama, Jiraporn Srisala, Ratny Khiev, Diva January Aldama-Cano, Siripong Thitamadee, Kallaya Sritunyalucksana
To improve the efficacy of WSSV protection, multimeric (tetrameric) recombinant VP28 (4XrVP28) was produced and tested in comparison with those of monomeric VP28 (1XrVP28). In vitro binding of either 1XrVP28 or 4XrVP28 to shrimp hemocyte surface was evident as early as 10 min after protein inoculation. Similar results were obtained in vivo when shrimp were injected with recombinant proteins that the proteins bound to the hemocyte surface could be detected since 5 min after injection. Comparison of the WSSV protection efficiencies of 1XrVP28 or 4XrVP28 were performed by injection the purified 1XrVP28 or 4XrVP28 (22...
May 22, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28545959/serpin-14-negatively-regulates-prophenoloxidase-activation-and-expression-of-antimicrobial-peptides-in-chinese-oak-silkworm-antheraea-pernyi
#3
Saima Kausar, Muhammad Nadeem Abbas, Cen Qian, Baojian Zhu, Yu Sun, Yuxuan Sun, Lei Wang, Guoqing Wei, Iram Maqsood, Chao-Liang Liu
Genes encoding proteins of serpins superfamily are widely distributed in invertebrates. In insects, serpins play important roles in regulating immune responses and other physiological processes. Here, we report the cloning and characterization of cDNA of Apserpin-14 from Chinese oak silkworm (Antheraea pernyi). The Apserpin-14 gene contains 1206 bp open reading frame, encoding a predicted 401 amino acid residue protein. We expressed the recombinant Apserpin-14 protein in Escherichia coli and then purified protein was used to prepare rabbit anti-Apserpin-14 polyclonal antibodies...
May 22, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28528959/role-of-astakine1-in-regulating-transglutaminase-activity
#4
Ratchanok Sirikharin, Kingkamon Junkunlo, Kenneth Söderhäll, Irene Söderhäll
Transglutaminase (TGase) has been implicated in maintaining the undifferentiated stage of hematopoietic stem cells (HSC) in the crayfish Pacifastacus leniusculus. TGase activity has been reported to be regulated by astakine1, an essential crayfish cytokine for inducing new hemocyte synthesis in hematopoietic tissue (HPT). Here, the role of astakine1 in TGase activity regulation and clotting protein (CP) cross-linking was characterized. A reduction in TGase activity was observed by the addition of purified astakine1 in vitro for both endogenous crayfish TGase and a commercial purified guinea pig liver TGase...
May 18, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28526442/comparative-study-of-interleukin-17c-il-17c-and-il-17d-in-large-yellow-croaker-larimichthys-crocea-reveals-their-similar-but-differential-functional-activity
#5
Yang Ding, Jingqun Ao, Xinhua Chen
Interleukin 17 (IL-17) family members are key players in regulating the immune response in mammals. Here, we identified the IL-17C and IL-17D homologs from large yellow croaker (Larimichthys crocea), named LcIL-17C and LcIL-17D, respectively. The deduced LcIL-17C and LcIL-17D proteins possessed the typical IL-17 domain and shared a conserved arrangement of eight cysteine residues. Both LcIL-17C and LcIL-17Dc genes were constitutively expressed in all tissues examined, although at different levels. After challenge with Aeromonas hydrophila, the expression of LcIL-17C and LcIL-17D was significantly increased in gills, head kidney, and spleen...
May 16, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28522173/variation-in-the-immune-state-of-gammarus-pulex-crustacea-amphipoda-according-to-temperature-are-extreme-temperatures-a-stress
#6
Sophie Labaude, Yannick Moret, Frank Cézilly, Charel Reuland, Thierry Rigaud
Temperature is known to impact host-parasite interactions in various ways. Such effects are often regarded as the consequence of the increased metabolism of parasites with increasing temperature. However, the effect of temperature on hosts' immune system could also be a determinant. Here we assessed the influence of temperature on the immunocompetence of the crustacean amphipod Gammarus pulex. Amphipods play a key ecological role in freshwater ecosystems that can be altered by several parasites. We investigated the consequences of three weeks of acclimatization at four temperatures (from 9 °C to 17 °C) on different immunological parameters...
May 15, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28512012/crustins-are-distinctive-members-of-the-wap-containing-protein-superfamily-an-improved-classification-approach
#7
REVIEW
Francisco Vargas-Albores, Marcel Martínez-Porchas
Crustins are considered effector molecules of innate immunity in arthropods, and classification schemes have been proposed over the last 10 years. However, classification problems have emerged: for example, proteins that have been well identified as members of a particular category have also been classified as crustins. Therefore, the objective of this manuscript was to analyze and, based on solid arguments, improve the original proposed nomenclature to make crustins a distinctive group of antibacterial proteins...
May 13, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28506725/the-first-invertebrate-nfil3-transcription-factor-with-role-in-immune-defense-identified-from-the-hong-kong-oyster-crassostrea-hongkongensis
#8
Jun Li, Yang Zhang, Yuehuan Zhang, Fan Mao, Zhiming Xiang, Shu Xiao, Haitao Ma, Ziniu Yu
NFIL3 (nuclear factor interleukin 3-regulated) is a basic leucine zipper type transcription factor that mediates a variety of immune responses in vertebrates. However, the sequence information and function of NFIL3 homologs in invertebrates, especially mollusks, remains unknown. In the present study, the first NFIL3 homolog was identified in a marine mollusk, Crassostrea hongkongensis (designated as ChNFIL3), followed by its functional characterization. The full-length cDNA of ChNFIL3 is 2221 bp and consists of an open reading frame (ORF) of 1536 bp that encodes a polypeptide of 551 amino acids...
May 12, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28502652/comparative-and-evolutionary-analysis-of-an-adapter-molecule-myd88-in-invertebrate-metazoans
#9
Yipeng Ren, Junli Xue, Huanhuan Yang, Baoping Pan, Wenjun Bu
The myeloid differentiation factor 88 (MyD88) is an essential adapter in Toll-like receptor (TLR) signalling pathways, with TLR the first pattern-recognition receptor (PRR) that was discovered in Drosophila. In the present study, a MyD88 gene was identified and characterized from a commercially important shellfish, Scapharca subcrenata, including a DEATH domain and TIR domain conserved within other molluscs. Furthermore, comparative genomic evidence revealed that MyD88 was of different lengths and contained quantitative exon and intron regions, which might be involved in specific mechanisms...
May 11, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28502651/rack-1-a-multifaceted-regulator-is-required-for-c-%C3%A2-elegans-innate-immunity-against-s-%C3%A2-flexneri-m9ot-infection
#10
Shanmugam Marudhupandiyan, Udayakumar Prithika, Boopathi Balasubramaniam, Krishnaswamy Balamurugan
The nematode C. elegans has the ability to clear off bacterial colonization in the intestine using pathogen specific innate immune response. Here, we show that C. elegans RACK-1 has been vital in determining the survival of worms under specific pathogenic infection. Among various pathogens tested, S. flexneri M9OT (SF) exhibited highest pathogenicity by killing rack-1 mutant worm-VC3013 earlier when compared to WT. The expression level of rack-1 mRNA was found to be decreased and it further indicated that the host translational event appeared to be affected during SF infection...
May 11, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28502650/crayfish-immunity-recent-findings
#11
Lage Cerenius, Kenneth Söderhäll
Freshwater crayfish is an important commodity as well as a successful model for studies on crustacean immunity. Due to the ease with which they are kept and the available methods for hemocyte separation and culture they have proven to be very useful. Here, recent progress regarding pattern recognition, immune effector production and antiviral mechanisms are discussed. Several cases of functional resemblance between vertebrate complement and the crayfish immune reactions are highlighted.
May 11, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28501515/shrimp-humoral-responses-against-pathogens-antimicrobial-peptides-and-melanization
#12
Anchalee Tassanakajon, Vichien Rimphanichyakit, Suwattana Visetnan, Piti Amparyup, Kunlaya Somboonwiwat, Walaiporn Charoensapsri, Sureerat Tang
Diseases have caused tremendous economic losses and become the major problem threatening the sustainable development of shrimp aquaculture. The knowledge of host defense mechanisms against invading pathogens is essential for the implementation of efficient strategies to prevent disease outbreaks. Like other invertebrates, shrimp rely on the innate immune system to defend themselves against a range of microbes by recognizing and destroying them through cellular and humoral immune responses. Detection of microbial pathogens triggers the signal transduction pathways including the NF-κB signaling, Toll and Imd pathways, resulting in the activation of genes involved in host defense responses...
May 10, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28499858/enhanced-behavioral-immune-defenses-in-a-c-%C3%A2-elegans-c-type-lectin-like-domain-gene-mutant
#13
Barbara Pees, Anke Kloock, Rania Nakad, Camilo Barbosa, Katja Dierking
C-type lectin-like domain (CTLD) proteins occupy crucial functions in the immune system of vertebrates, but their role in invertebrate immunity is much less understood. The nematode Caenorhabditis elegans possesses a highly diverse CTLD protein encoding (clec) gene repertoire. A role of C. elegans clec genes in pathogen defense is always assumed, yet experimental evidence for clec immune function is rare. To systematically test the potential function of clec genes in the C. elegans defense against pathogens, we screened 39 clec mutants for survival on the Gram-positive pathogen Bacillus thuringiensis (BT18247) and 37 clec mutants on the Gram-negative pathogen Pseudomonas aeruginosa (PA14)...
May 10, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28499857/preface-to-the-special-issue-impact-of-omics-on-comparative-immunology
#14
EDITORIAL
Pierre Boudinot, Unni Grimholt, John Hansen
No abstract text is available yet for this article.
May 9, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28487235/transcriptome-wide-analysis-of-micrornas-in-branchiostoma-belcheri-upon-vibrio-parahemolyticus-infection
#15
Ping Jin, Shengjie Li, Lianjie Sun, Caiyun Lv, Fei Ma
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that participate in diverse biological processes via regulating expressions of target genes at post-transcriptional level. Amphioxus, as modern survivor of an ancient chordate lineage, is a model organism for comparative genomics study. However, miRNAs involved in regulating immune responses in Branchiostoma belcheri are largely unclear. Here, we systematically investigated the microRNAs (miRNAs) involved in regulating immune responses in the cephalochordate amphioxus (Branchiostoma belcheri) through next-generation deep sequencing of amphioxus samples infected with Vibrio parahemolyticus...
May 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28487234/anti-inflammatory-action-of-angiotensin-1-7-in-experimental-colitis-may-be-mediated-through-modulation-of-serum-cytokines-chemokines-and-immune-cell-functions
#16
Maitham A Khajah, Maryam M Fateel, Kethireddy V Ananthalakshmi, Yunus A Luqmani
We recently demonstrated Ang 1-7 reduced inflammation in the dextran sulfate sodium (DSS) colitis model. In this study we examined the effect of Ang 1-7 on modulation of plasma levels of selected cytokines and chemokines and immune cell effector functions (apoptosis, chemotaxis and superoxide release) in vitro. The degree of neutrophil recruitment to the colon was assessed by immunofluorescence and myeloperoxidase activity. Daily Ang 1-7 treatment at 0.01 mg/kg dose which previously ameliorated colitis severity, showed a significant reduction in circulating levels of several cytokines and chemokines, and neutrophil recruitment to the colonic tissue...
May 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28483486/corrigendum-to-characterization-of-molecular-properties-and-regulatory-pathways-of-crustinpm1-and-crustinpm7-from-the-black%C3%A2-tiger-shrimp-penaeus-monodon-dev-comp-immunol-67%C3%A2-2017-%C3%A2-18-29
#17
Sopacha Arayamethakorn, Premruethai Supungul, Anchalee Tassanakajon, Kuakarun Krusong
No abstract text is available yet for this article.
May 5, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28479346/cqtoll-participates-in-antiviral-response-against-white-spot-syndrome-virus-via-induction-of-anti-lipopolysaccharide-factor-in-red-claw-crayfish-cherax-quadricarinatus
#18
Yan-Yao Li, Xiao-Xiao Chen, Feng-Yu Lin, Qiu-Fan Chen, Xing-Yuan Ma, Hai-Peng Liu
It is well known that Tolls/Toll like receptors (TLRs), a family of pattern recognition receptors, play important roles in immune responses. Previously, we found that a Toll transcript was increased in a transcriptome library of haematopoietic tissue (Hpt) cells from the red claw crayfish Cherax quadricarinatus post white spot syndrome virus infection. In the present study, a full-length cDNA sequence of Toll receptor (named as CqToll) was identified with 3482 bp which contained an open reading frame of 3021 bp encoding 1006 amino acids...
May 4, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28479345/a-bcwd-resistant-line-of-rainbow-trout-exhibits-higher-abundance-of-igt-b-cells-and-heavy-chain-tau-transcripts-compared-to-a-susceptible-line-following-challenge-with-flavobacterium-psychrophilum
#19
Patty Zwollo, Erin Hennessey, Catherine Moore, David P Marancik, Gregory D Wiens, Lidia Epp
Bacterial Cold Water Disease (BCWD) is a common, chronic disease in rainbow trout, and is caused by the gram-negative bacterium Flavobacterium psychrophilum (Fp). Through selective breeding, the National Center for Cool and Cold Water Aquaculture has generated a genetic line that is highly resistant to Fp challenge, designated ARS-Fp-R (or R-line), as well as a susceptible "control" line, ARS-Fp-S (S-line). In previous studies, resistance to Fp had been shown to correlate with naive animal spleen size, and further, naïve R-line trout had been shown to have a lower abundance of IgM(+) and IgM(++) cells compared to S-line fish...
May 4, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28479344/characterization-and-antimicrobial-evaluation-of-sppr-amp1-a-proline-rich-antimicrobial-peptide-from-the-mud-crab-scylla-paramamosain
#20
Chanprapa Imjongjirak, Pawanrat Amphaiphan, Walaiporn Charoensapsri, Piti Amparyup
Antimicrobial peptide (AMP) is an important molecule in the innate immune system. Here, we report the cloning and functional studies of proline-rich AMPs (PR-AMPs) from the three species of mud crab: Scylla paramamosain, S. serrata, and the swimming crab Portunus pelagicus. The deduced peptides revealed that they contain the putative signal peptides and encode for mature peptides, which contain sequence architecture similar to a 6.5-kDa proline-rich AMP of the shore crab, Carcinus maenas which showed similarity with the bactenecin7...
May 4, 2017: Developmental and Comparative Immunology
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