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Developmental and Comparative Immunology

Kai Luo, Shuhuan Zhang, Dongdong Tang, Lihai Xia, Weihua Gao, Guangming Tian, Zhitao Qi, Qiaoqing Xu, Wenbing Zhang
Teleost fish have more complex interferon receptor systems than mammals. In the present study, genes encoding four cytokine receptor family B (CRFBs) and two interferon gamma receptors (IFNGRs) in Dabry's sturgeon (Acipenser dabryanus) were identified by RNA-sequencing. Sequence analysis revealed that the Dabry's sturgeon CRFBs and IFNGRs contained several conserved characteristics features, including signal peptides and a transmembrane domain. Phylogenetic analysis suggested that they belong to the CRFB3, CRFB5, and IFNGR protein families, and were named CRFB3a, CRFB3b, CRFB5a, CRFB5b, IFNGR1, and IFNGR2...
March 16, 2018: Developmental and Comparative Immunology
Fan-Xiang Gao, Wei-Jia Lu, Yang Wang, Qi-Ya Zhang, Yi-Bing Zhang, Cheng-Yan Mou, Zhi Li, Xiao-Juan Zhang, Chao-Wei Liu, Li Zhou, Jian-Fang Gui
Diverse immunoglobulin (Ig) domain-containing protein (DICP) family is a novel bony fish-specific multi-gene family encoding diversified immune receptors. However, their function and the implication of binding partners remain unknown. In this study, we first identified 28 DICPs from three gibel carp gynogenetic clones and revealed their high variability and clone-specific feature. After crucian carp herpesvirus (CaHV) infection, these DICPs were significantly upregulated in head kidney, kidney and spleen. The up-regulation folds in clone A+ , F and H were related to the susceptibility to CaHV, progressively increasing from resistant clone to susceptible clone...
March 16, 2018: Developmental and Comparative Immunology
Sandra Vreman, Gael Auray, Huub F J Savelkoul, Annemarie Rebel, Artur Summerfield, Norbert Stockhofe-Zurwieden
The present study investigated the innate immune response in vitro to determine porcine neonate responses with Toll-like receptor (TLR)2 ligand (Pam3Cys) or TLR9 ligand (CpG) and compare these with adults. We identified the same phenotypically defined dendritic cells (DC) subsets and DC proportions in porcine neonate and adult blood by flow cytometry, which were plasmacytoid DCs (pDCs): CD14- CD4+ CD172a+ CADM1- ) and conventional DCs (cDCs), the latter being further divided into a cDC1 (CD14- CD4- CD172alow CADM1+ ) and a cDC2 (CD14- CD4- CD172a+ CADM1+ ) subset...
March 16, 2018: Developmental and Comparative Immunology
Pakpoom Boonchuen, Phattarunda Jaree, Anchalee Tassanakajon, Kunlaya Somboonwiwat
Acute hepatopancreatic necrosis disease, AHPND, caused by a specific strain of Vibrio parahaemolyticus (VPAHPND ), results in great loss of global shrimp production. Despite this, studies on shrimp defense mechanisms protecting against AHPND are few. In this study, suppression subtractive hybridization (SSH) was performed to identify differentially expressed genes from white shrimp Litopenaeus vannamei hepatopancreas upon VPAHPND infection at the early stages: 3 and 6 h post challenge and in the late stage at 48 h post challenge...
March 15, 2018: Developmental and Comparative Immunology
Yongjie Liu, Fujun Hou, Zhaoying Qian, Xiaolin Liu
The clade B serpins contain predominantly intracellular proteins and were reported to be involved in multiple biological functions, especially in inflammation and immune system function. However, studies about the role of the invertebrate intracellular serpins in immune responses were still deficient. Therefore, this paper focused on the functional characterization of LvserpinB3 in white shrimp Litopenaeus vannamei. The pAc5.1-LvserpinB3-EGFP vector was transfected into Drosophila Schneider 2 (S2) cells to analyze the subcellular localization of LvserpinB3, and fluorescent imaging showed that LvserpinB3 were mainly localized to the mitochondria...
March 14, 2018: Developmental and Comparative Immunology
Zhaoqun Liu, Lingling Wang, Yunchen Yan, Yan Zheng, Wenjing Ge, Meijia Li, Weilin Wang, Xiaorui Song, Linsheng Song
Dopamine (DA), a significant member of catecholamines, is reported to induce biomineralization of calcium carbonate vaterite microspheres via dopamine receptor (DR) in bivalves, implying the modulation of dopaminergic system on shell formation during larval development. In this research, a homologue of D1 type DR (CgD1DR-1) was identified from oyster Crassostrea gigas, whose full length cDNA was 1197 bp. It was widely expressed in various tissues of C. gigas, with the significantly higher levels in hepatopancreas, mantle, muscle and gill...
March 14, 2018: Developmental and Comparative Immunology
Sisi Gao, Pierre J Deviche
Stress-induced inhibition of innate immune activity is widespread in free-ranging birds, but the mechanisms that are responsible for this inhibition are poorly understood. We previously demonstrated that an increase in plasma corticosterone (CORT), the primary avian glucocorticoid, is necessary for the inhibition of natural antibody- and complement-mediated as well as bactericidal activities to occur during stress. Here we investigated the role of glucocorticoid receptors in stress-induced inhibition of natural antibody- and complement-mediated activities and bactericidal activity within non-genomic (<10 min) and genomic (<120 min) time frames in male House Sparrows, Passer domesticus...
March 9, 2018: Developmental and Comparative Immunology
Peng Zhang, Yu Liu, Min Wang, Miren Dong, Zhaoqun Liu, Zhihao Jia, Weilin Wang, Anguo Zhang, Lingling Wang, Linsheng Song
Iron-sulphur clusters (ISCs), one of the oldest and most versatile cofactors of proteins, are involved in catalysis reactions, electron transport reactions, regulation processes as well as sensing of ambient conditions. Iron-sulphur cluster assembly protein (IscA) is a scaffold protein member of ISC formation system, which plays a significant role in the assembly and maturation process of ISC proteins. In the present study, the cDNA sequence of iron-sulphur cluster assembly protein 2 (designated as EsIscA2) was cloned from Eriocheir sinensis...
March 9, 2018: Developmental and Comparative Immunology
Regla M Medina-Gali, María Del Mar Ortega-Villaizán, Luis Mercado, Beatriz Novoa, Julio Coll, Luis Perez
The antiviral effects of beta-glucan, an immunostimulatory agent were studied in zebrafish both in vitro and in vivo. Here we show that zebrafish ZF4 cells as well as whole fish primed with yeast β-glucan zymosan exhibited increased cytokine expression and elevated response to spring viremia of carp virus (SVCV) infection. In vitro, previous treatment of β-glucan enhanced ZF4 cell viability against SVCV infection which is associated to the activation of interferon signaling pathway and inflammatory cytokines gene expression...
March 7, 2018: Developmental and Comparative Immunology
Fumihiko Katakura, Yuji Sugie, Kiyotaka Hayashi, Kohei Nishiya, Jiro Miyamae, Masaharu Okano, Teruyuki Nakanishi, Tadaaki Moritomo
The development of mammalian megakaryocytes and platelets is regulated by numerous cytokine signals, primarily through the thrombopoietin (TPO)/c-MPL axis. Although non-mammalian vertebrates are known to possess nucleated thrombocytes functionally equivalent to mammalian platelets, the dynamics of the thrombocyte development remains unclear. Here we identified TPO and a splice variant (TPO-v) caused by the intron retention in common carp (Cyprinus carpio). Both the tpo and its variant transcripts were highly expressed in heart and liver...
March 6, 2018: Developmental and Comparative Immunology
Guang-Ping Liu, Li-Xin Xiang, Tong Shao, Ai-Fu Lin, Jian-Zhong Shao
Peroxiredoxin 1 (Prdx1/Prx1), a ubiquitous antioxidant enzyme involved in preventing oxidative damage and maintaining redox homeostasis, is essential for various cellular activities. Extracellular Prdx1 also plays important roles in innate immune responses. However, little is known about the regulatory functions of Prdx1 in adaptive immunity. In the present study, we report the stimulatory role of Prdx1 in the initiation of adaptive humoral immunity in a zebrafish model. Administration of Prdx1 protein to zebrafish significantly induced the expression of TNF-α, IL-1β, and IL-6 by the Toll-like-receptor-4a-mediated NF-κB signaling pathway and enhanced the activation of MHC-II+ antigen-presenting cells, CD4+ T cells, and mIgM+ B cells...
March 5, 2018: Developmental and Comparative Immunology
Yu Liu, Peng Zhang, Weilin Wang, Miren Dong, Min Wang, Changhao Gong, Zhihao Jia, Zhaoqun Liu, Anguo Zhang, Lingling Wang, Linsheng Song
DM9 is a novel protein domain with unknown function originally discovered in Drosophila melanogaster. Recently, a protein harboring DM9 repeats was identified as mannose-specific lectin (CgCGL1, renamed as CgDM9CP-1) from the Pacific oyster Crassostrea gigas. In the present study, another DM9 containing protein was identified from oyster C. gigas (designated as CgDM9CP-2). The open reading frame of CgDM9CP-2 gene was of 432 bp, encoding a polypeptide of 143 amino acids with two tandem DM9 repeats. The deduced amino acid sequence of CgDM9CP-2 shared 60...
March 5, 2018: Developmental and Comparative Immunology
Xiao-Lu Xie, Xue-Jiao Chang, Yan Gao, Dong-Li Li, Ling-Ke Liu, Man-Jun Liu, Ke-Jian Wang, Hai-Peng Liu
Influenza A virus non-structural-1A binding protein (named as Ns1abp) was originally identified as a host protein from human that bound to the viral NS-1 protein. In our previous study, the expression of an Ns1abp-like gene (denoted as CqNs1abp-like gene) was found to be up-regulated in a transcriptome library from the haematopoietic tissue (Hpt) cells of red claw crayfish Cherax quadricarinatus post white spot syndrome virus (WSSV) infection. To elucidate the role of CqNs1abp-like gene involved in WSSV infection, we cloned the CqNs1abp-like gene in which the open reading frame was 2232 bp, encoding 743 amino acids with two typical domains of one BTB (Broad-Complex, Tramtrack and Bric a brac) domain at N-terminal and six Kelch domains at C-terminal...
March 3, 2018: Developmental and Comparative Immunology
Sarah J Baker, Mark E Merchant
The innate immune system functions to quickly respond to pathogens and is likely the primary line of defense for ectothermic vertebrates. Snake populations appear to be in widespread decline globally, but despite the threats from emerging pathogens, very little work has been conducted to characterize their basic immune function. We used a wide-ranging snake species, the Prairie Rattlesnake (Crotalus viridis), to measure effects of snake plasma on the growth of eight bacterial species. Additionally, we quantified bacterial killing ability and kinetics of the immune response...
March 3, 2018: Developmental and Comparative Immunology
Débora Torrealba, Joan Carles Balasch, Manuel Criado, Lluís Tort, Simon Mackenzie, Nerea Roher
The inflammatory reflex modulates the innate immune system, keeping in check the detrimental consequences of overstimulation. A key player controlling the inflammatory reflex is the alpha 7 acetylcholine receptor (α7nAChR). This receptor is one of the signalling molecules regulating cytokine expression in macrophages. In this study, we characterize a novel teleost α7nAChR. Protein sequence analysis shows a high degree of conservation with mammalian orthologs and trout α7nAChR has all the features and essential amino acids to form a fully functional receptor...
March 1, 2018: Developmental and Comparative Immunology
Alexander M Gorbushin
The evolution of complement system in invertebrates is poorly investigated. While the repertoire of complement genes in several Ecdysozoa lineages is found substantially different from that of Deuterostomia, the composition and function of the complement in the second protostome lineage, Lophotrochozoa, remains unclear. Here we report the general description of new transcriptomic data on the common periwinkle, Littorina littorea, and trace the evolutionary trajectories of the ancestral proto-complement repertoire...
February 28, 2018: Developmental and Comparative Immunology
Pengzhi Qi, Bin Wu, Baoying Guo, Chuan Zhang, Kaida Xu
Complement is a complex innate immune surveillance system, playing a key role in host homeostasis, inflammation, and in the defense against pathogens. Complement regulators are crucial to prevent the injudicious production of these mediators and potential injury to self tissues. Here, we identified the complement factor H (CFH) and its related gene 2 (CFHR2) homologs from large yellow croaker (Larimichthys crocea), named LcCfh and LcCfhr2, respectively. The deduced LcCfh and LcCfhr2 proteins shared significant structural similarities and identified codes for a polypeptide consisting of various numbers of highly conserved SCR domains...
February 26, 2018: Developmental and Comparative Immunology
Rebeca Moreira, Patricia Pereiro, Pablo Balseiro, Massimo Milan, Marianna Pauletto, Luca Bargelloni, Beatriz Novoa, Antonio Figueras
Mediterranean mussels are a worldwide spread bivalve species with extraordinary biological success. One of the reasons of this success could be the reproduction strategy of bivalves, characterized by the presence of trochophore larvae. Larval development in bivalves has been a topic of raising interest in the scientific community but it deserves much more attention. The principal objective of this work was to study the transcriptomic profile of the ontogeny of Mytilus galloprovincialis analyzing the gene expression in different developmental stages, from oocytes to juveniles...
February 23, 2018: Developmental and Comparative Immunology
Liangliang Mu, Xiaoxue Yin, Yanhui Xiao, Xia Bian, Yanjian Yang, Liting Wu, Jianmin Ye
Collectins, a subfamily of the C-type lectins, are able to bind non-self glycoconjugates on the surface of microorganisms and inhibit infection by direct neutralization, agglutination and/or opsonization, which play important roles in innate immunity. In this study, a CL11X1-like collectin (OnCL11X1) was identified from Nile tilapia (Oreochromis niloticus) and characterized at expression and agglutination functional levels. The open reading frame of OnCL11X1 is 840 bp of nucleotide sequence encoding polypeptides of 279 amino acids...
February 24, 2018: Developmental and Comparative Immunology
Xiaoyuan Wei, Limin Wang, Wanwei Sun, Ming Zhang, Hongyu Ma, Yueling Zhang, Xinxu Zhang, Shengkang Li
As pattern recognition receptors, C-type lectins (CTLs) play important roles in immune system of crustaceans through identifying and binding to the conservative pathogen-associated molecular patterns (PAMPs) on pathogen surfaces. In this study, a new CTL, SpCTL-B, was identified from the hemocytes of mud crab Scylla paramamosain. The full-length of SpCTL-B cDNA was 1278 bp with an open reading frame (ORF) of 348 bp. The predicted SpCTL-B protein contains a single carbohydrate-recognition domain (CRD). SpCTL-B transcripts were distributed in all examined tissues with the highest levels in hepatopancreas...
February 21, 2018: Developmental and Comparative Immunology
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