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https://www.readbyqxmd.com/read/29339074/identification-and-characterization-of-a-doublesex-gene-which-regulates-the-expression-of-insulin-like-androgenic-gland-hormone-in-fenneropenaeus-chinensis
#1
Shihao Li, Fuhua Li, Kuijie Yu, Jianhai Xiang
The doublesex and its homologue genes are important regulators of sexual differentiation which are conserved among animal kingdom. In the present study, we reported a doublesex gene (designated as FcDsx) identified from the Chinese shrimp F. chinensis. The gene structure, nucleotide and deduced amino acid sequences of FcDsx were characterized. The results showed that the deduced amino acid sequence of FcDsx had the common features of Dsx proteins, including a doublesex/male abnormal 3 (DM) domain, an oligomerization domain and a predicted monopartite nuclear localization signal...
January 12, 2018: Gene
https://www.readbyqxmd.com/read/29339073/the-human-gcom1-complex-gene-interacts-with-the-nmda-receptor-and-internexin-alpha
#2
Raymond S Roginski, Chi W Lau, Phillip P Santoiemma, Sara J Weaver, Peicheng Du, Patricia Soteropoulos, Jay Yang
The known functions of the human GCOM1 complex hub gene include transcription elongation and the intercalated disk of cardiac myocytes. However, in all likelihood, the gene's most interesting, and thus far least understood, roles will be found in the central nervous system. To investigate the functions of the GCOM1 gene in the CNS, we have cloned human and rat brain cDNAs encoding novel, 105 kDa GCOM1 combined (Gcom) proteins, designated Gcom15, and identified a new group of GCOM1 interacting genes, termed Gints, from yeast two-hybrid (Y2H) screens...
January 12, 2018: Gene
https://www.readbyqxmd.com/read/29339072/deep-sequencing-of-a-qtl-rich-region-spanning-128-136mbp-of-pig-chromosome-15
#3
K Piórkowska, K Żukowski, K Ropka-Molik, M Tyra
The present study shows the characterization of the chromosome 15 (SSC15) region that is highly rich in quantitative traits loci (QTLs) associated with pork quality, growth performance, fat and meat carcass contents. The analytic method that was utilized included targeted enrichment DNA sequencing and RNA hybridisation probes. The research included two pig breeds (Puławska and Polish Landrace) that are significantly different in terms of carcass and meat quality features. Filtered sequences were aligned to the Sscrofa10...
January 12, 2018: Gene
https://www.readbyqxmd.com/read/29339071/mir-155-5p-promotes-fibroblast-cell-proliferation-and-inhibits-foxo-signaling-pathway-in-vulvar-lichen-sclerosis-by-targeting-foxo3-and-cdkn1b
#4
Lina Ren, Yi Zhao, Xiaoxi Huo, Xin Wu
Vulvar lichen sclerosis (VLS) is a chronic inflammatory skin disorder. Evidence is accumulating that microRNAs (miRNAs) exert crucial roles in initiation and development of a wide range of human diseases. MiR-155-5p has been frequently reported to be implicated in the tumorigenesis and progression of multiple types of cancers, however, its biological role in VLS remains unclear. This study aimed to explore the role of miR-155-5p in VLS and clarify the potential molecular mechanisms involved. In the present study, miR-155-5p was observed to be significantly upregulated in VLS tissues...
January 12, 2018: Gene
https://www.readbyqxmd.com/read/29339070/involvement-of-interferon-regulatory-factor-3-from-the-barbel-chub-squaliobarbus-curriculus-in-the-immune-response-against-grass-carp-reovirus
#5
Ronghua Wang, Yaoguo Li, Zhiyu Zhou, Qiaolin Liu, Lingbing Zeng, Tiaoyi Xiao
The barbel chub Squaliobarbus curriculus is an important commercial fish species in China, and has shown significant resistance to grass carp reovirus (GCRV). In this study, the cDNA sequence of interferon regulatory factors 3 (IRF3) from Squaliobarbus curriculus, designated as ScIRF3, was cloned, and its effect against GCRV was investigated. The full-length 1837 base pair (bp) cDNA of ScIRF3 contained a complete open reading frame of 1374 bp and encoded a putative polypeptide of 457 amino acid residues. The ScIRF3 protein contained conserved domains, including an N-terminal DNA-binding domain, a C-terminal IRF association domain, and a serine-rich domain...
January 12, 2018: Gene
https://www.readbyqxmd.com/read/29339069/multiple-homologous-genes-knockout-ko-by-crispr-cas9-system-in-rabbit
#6
Huan Liu, Tingting Sui, Di Liu, Tingjun Liu, Mao Chen, Jichao Deng, Yuanyuan Xu, Zhanjun Li
The CRISPR/Cas9 system is a highly efficient and convenient genome editing tool, which has been widely used for single or multiple gene mutation in a variety of organisms. Disruption of multiple homologous genes, which have similar DNA sequences and gene function, is required for the study of the desired phenotype. In this study, to test whether the CRISPR/Cas9 system works on the mutation of multiple homologous genes, a single guide RNA (sgRNA) targeting three fucosyltransferases encoding genes (FUT1, FUT2 and SEC1) was designed...
January 12, 2018: Gene
https://www.readbyqxmd.com/read/29339068/a-new-mirna-regulator-mir-672-reduces-cardiac-hypertrophy-by-inhibiting-jun-expression
#7
Yili Lu, Fangli Wu
Cardiac hypertrophy is one of the initial symptoms of many heart diseases. We found that miR-672-5p may participate in the regulation of heart disease development in mouse, but the association between miR-672-5p and cardiac hypertrophy remains unclear. In the present study, we found that the abundance of miR-672-5p decreased in hypertrophic cardiomyocytes induced by phenylephrine, angiotensin II (Ang II) and insulin-like growth factor 1. Putative target genes of miR-672-5p were identified using four pipelines, miRWalk, miRanda, RNA22 and Targetscan, and a total of 834 genes were predicted by all four pipelines...
January 12, 2018: Gene
https://www.readbyqxmd.com/read/29339067/molecular-characterization-and-function-of-%C3%AE-n-acetylglucosaminidase-from-ridgetail-white-prawn-exopalaemon-carinicauda
#8
Yuying Sun, Jiquan Zhang, Jianhai Xiang
Chitin degradation is catalyzed by a two-component chitinolytic enzyme system, chitinase and β-N-acetylglucosaminidase (NAGase). In this paper, the full-length cDNA sequence encoding NAGase (EcNAG) was obtained from Exopalaemon carinicauda. The deduced amino acid sequence of EcNAG open reading frame (ORF) contained one Glycohydro_20b2 domain and one Glyco_hydro_20 domain. Based on the cDNA sequence, the genomic structure of EcNAG was characterized and it was composed of six exons and five introns. EcNAG mRNA majorly expressed in the hepatopancreas and epidermis...
January 12, 2018: Gene
https://www.readbyqxmd.com/read/29337088/cross-regulatory-network-in-pseudomonas-aeruginosa-biofilm-genes-and-tio2-anatase-induced-molecular-perturbations-in-key-proteins-unraveled-by-a-systems-biology-approach
#9
Rani Anupama, S Sajitha Lulu, Amitava Mukherjee, Subramanian Babu
A systems biology approach was used to study all the biofilm related genes of P. aeruginosa PAO1, and the interaction of titanium dioxide (TiO2) anatase with biofilm related proteins. Among the 71 genes, the interactions of all the nodal pairs were extracted by STRING 10.5 database. The inter-relationship among these genes was predicted by constructing complete PPI network and visualized in Cytoscape v 3.4.0. Total number of nodes of the network was found to be 335 and edges were 795. The network was further investigated for its clusters and the best cluster was further analyzed for the hub proteins which significantly contribute in cross-regulation of the biofilm related process...
January 11, 2018: Gene
https://www.readbyqxmd.com/read/29337087/genetic-polymorphisms-of-pharmacogenomic-vip-variants-in-the-yi-population-from-china
#10
Mengdan Yan, Dianzhen Li, Guige Zhao, Jing Li, Fanglin Niu, Bin Li, Peng Chen, Tianbo Jin
INTRODUCTION: Drug response and target therapeutic dosage are different among individuals. The variability is largely genetically determined. With the development of pharmacogenetics and pharmacogenomics, widespread research have provided us a wealth of information on drug-related genetic polymorphisms, and the very important pharmacogenetic (VIP) variants have been identified for the major populations around the world whereas less is known regarding minorities in China, including the Yi ethnic group...
January 11, 2018: Gene
https://www.readbyqxmd.com/read/29331485/impact-of-9p21-3-region-and-atherosclerosis-related-genes-variants-on-long-term-recurrent-hard-cardiac-events-after-a-myocardial-infarction
#11
German J Osmak, Boris V Titov, Natalia A Matveeva, Vitalina V Bashinskaya, Roman M Shakhnovich, Tatiana S Sukhinina, Nino G Kukava, Mikhail Ya Ruda, Olga O Favorova
Atherosclerotic coronary artery disease (CAD) and myocardial infarction (MI) as its most severe clinical complication remain the leading causes of mortality in the majority of countries. Despite the progress in the treatment of MI, quite often the patients, after the first-time MI, develop subsequently a variety of adverse cardiovascular events. In this retrospective study we evaluated the contribution of allelic variations in 9p21.3 locus and in 21 atherogenesis-related genes to the development of hard cardiac events in a cohort of patients of Russian ethnicity after the first acute MI during long-term follow-up (7-10 years)...
January 10, 2018: Gene
https://www.readbyqxmd.com/read/29331484/novel-biomolecular-information-in-rotenone-induced-cellular-model-of-parkinson-s-disease
#12
D Lin, Y Liang, D Zheng, Y Chen, X Jing, M Lei, Z Zeng, T Zhou, X Wu, S Peng, K Huang, L Yang, S Xiao, J Liu, E Tao
In order to uncover the remarkable pathogenic genes or molecular pathological process in Parkinson's disease (PD), we employed a microarray analysis upon the cellular PD model induced by rotenone. Compared to the control group, 2174 genes were screened out to be expressed differently in the rotenone-induced group by certain criterion. GO analysis and the pathways analysis showed the significant enrichment of genes that were associated with the biological process of cell cycle, apoptotic process, organelle fusion, mitochondrial lesion, endoplasmic reticulum stress and so on...
January 10, 2018: Gene
https://www.readbyqxmd.com/read/29331483/gata4-is-a-transcriptional-regulator-of-r-spondin1-in-japanese-flounder-paralichthys-olivaceus
#13
Xiumei Liu, Zan Li, Bo Wang, He Zhu, Yuezhong Liu, Jie Qi, Quanqi Zhang
GATA4 is a well-known transcription factor of the GATA family implicated in regulation of sex determination and gonadal development in mammals. In this study, we cloned the full-length cDNA of Paralichthys olivaceus gata4 (Po-gata4). Phylogenetic, gene structure, and synteny analysis showed that Po-GATA4 is homologous to GATA4 of teleost and tetrapod. Po-gata4 transcripts were detected in Sertoli cells, spermatogonia, oogonia and oocytes, with higher transcript levels overall in the testis than the ovary. The promoter region of P...
January 10, 2018: Gene
https://www.readbyqxmd.com/read/29331482/genetic-basis-of-hearing-loss-in-spanish-hispanic-and-latino-populations
#14
REVIEW
Rahul Mittal, Amit P Patel, Desiree Nguyen, Debbie R Pan, Vasanti M Jhaveri, Jason R Rudman, Arjuna Dharmaraja, Denise Yan, Yong Feng, Prem Chapagain, David J Lee, Susan H Blanton, Xue Zhong Liu
Hearing loss (HL) is the most common neurosensory disorder affecting humans. The screening, prevention and treatment of HL require a better understanding of the underlying molecular mechanisms. Genetic predisposition is one of the most common factors that leads to HL. Most HL studies include few Spanish, Hispanic and Latino participants, leaving a critical gap in our understanding about the prevalence, impact, unmet health care needs, and genetic factors associated with hearing impairment among Spanish, Hispanic and Latino populations...
January 10, 2018: Gene
https://www.readbyqxmd.com/read/29331481/genetic-contribution-of-sun5-mutations-to-acephalic-spermatozoa-in-fujian-china
#15
Yan-Wei Sha, Xiaohui Xu, Zhi-Yong Ji, Shao-Bin Lin, Xu Wang, Ping-Ping Qiu, Yulin Zhou, Li-Bin Mei, Zhi-Ying Su, Lin Li, Ping Li
Acephalic spermatozoa is an extremely rare disease associated with primary infertility. A recent study showed that genetic alterations in the SUN5 gene lead to this disease, and SUN5 mutations could explain the disease in about half of the patients. Therefore, in the present study, to re-visit the genetic contribution of SUN5 mutations to acephalic spermatozoa, we recruited 15 unrelated affected individuals and screened the SUN5 gene for mutations by whole-exome sequencing (WES) and Sanger sequencing. Five of the 15 (33...
January 10, 2018: Gene
https://www.readbyqxmd.com/read/29331480/human-papillomavirus-hpv-18-genetic-variants-and-cervical-cancer-risk-in-taizhou-area-china
#16
Hui-Hui Xu, Ling-Zhi Zheng, Ai-Fen Lin, Shan-Shan Dong, Ze-Ying Chai, Wei-Hua Yan
Human papillomavirus (HPV) type 18 is predominantly associated with the development of cervical adenocarcinomas, whereas data on HPV18 genetic variability in China are limited. HPV18 genetic variants were formed phylogenetic tree, including lineages A, B, and C. We aimed to evaluate the diversity of HPV18 genetic variants by sequencing the entire E6, E7 and L1 genes. Between 2012 and 2015, a total of 138 (0.8%, 138/17669) women with single HPV18 infection were selected in this study. Finally, we observed 122 HPV18 isolates of the complete E6-E7-L1 sequences, and obtained 36 distinct variation patterns which the accession GenBank numbers as KY457805-KY457840...
January 10, 2018: Gene
https://www.readbyqxmd.com/read/29331479/molecular-characterization-and-expression-of-piwil1-and-piwil2-during-gonadal-development-and-treatment-with-hcg-and-lhrh-a2-in-odontobutis-potamophila
#17
Cheng Zhao, Wenxu Zhu, Shaowu Yin, Quanquan Cao, Hongye Zhang, Xin Wen, Guosong Zhang, Wanli Xie, Shuqiao Chen
Piwi proteins play an important regulatory role in germ cell division during gametogenesis and gonad development. In order to understand the function of Piwi genes in the reproductive process of the dark sleeper, we identified and characterized Piwil1 and Piwil2 from gonad tissue. The tissue distribution demonstrated that Piwils were highly expressed in the gonad of the dark sleeper. During gonad development, higher expression was observed in stage I of both the testes and ovaries than in subsequent stages at mRNA and protein levels...
January 10, 2018: Gene
https://www.readbyqxmd.com/read/29331478/comprehensive-analysis-of-lncrnas-and-mrnas-with-associated-co-expression-and-cerna-networks-in-c2c12-myoblasts-and-myotubes
#18
Rui Chen, Ting Jiang, Yanling She, Sidong Xie, Shanyao Zhou, Cheng Li, Jun Ou, Yulin Liu
Long non-coding RNAs (lncRNAs) are emerging as important regulators in the modulation of muscle development and muscle-related diseases. To explore potential regulators of muscle differentiation, we determined the expression profiles of lncRNAs and mRNAs in C2C12 mouse myoblast cell line using microarray analysis. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed to explore their function. We also constructed co-expression, cis/trans-regulation, and competing endogenous RNA (ceRNA) networks with bioinformatics methods...
January 10, 2018: Gene
https://www.readbyqxmd.com/read/29329929/detoxification-genes-polymorphisms-in-sids-exposed-to-tobacco-smoke
#19
Laura Filonzi, Cinzia Magnani, Anna Maria Lavezzi, Marina Vaghi, Luana Nosetti, Francesco Nonnis Marzano
The best hypothesis to explain Sudden Infant Death Syndrome (SIDS) pathogenesis is offered by the "triple risk model", which suggests that an interaction of different variables related to exogenous stressors and infant vulnerability may lead to the syndrome. Environmental factors are triggers that act during a particular sensible period, modulated by intrinsic genetic characteristics. Although literature data show that one of the major SIDS risk factors is smoking exposure, a specific involvement of molecular components has never been highlighted...
January 9, 2018: Gene
https://www.readbyqxmd.com/read/29329928/cloning-and-characterization-of-%C3%A2-6-%C3%A2-5-fatty-acyl-desaturase-fad-gene-promoter-in-the-marine-teleost-siganus-canaliculatus
#20
Yewei Dong, Jianhong Zhao, Junliang Chen, Shuqi Wang, Yang Liu, Qinghao Zhang, Cuihong You, Óscar Monroig, Douglas R Tocher, Yuanyou Li
The rabbitfish Siganus canaliculatus was the first marine teleost demonstrated to have the ability of biosynthesizing long-chain polyunsaturated fatty acids (LC-PUFA) from C18 PUFA precursors, and all genes encoding the key enzymes for LC-PUFA biosynthesis have been cloned and functionally characterized, which provides us a potential model to study the regulatory mechanisms of LC-PUFA biosynthesis in teleosts. As the primary step to clarify such mechanisms, present research focused on promoter analysis of gene encoding ∆6/∆5 fatty acyl desaturase (Fad), a rate-limiting enzyme catalyzing the first step in the conversion of C18 PUFA to LC-PUFA...
January 9, 2018: Gene
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