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Anton Karabinos
Intermediate filaments (IF) belong to major cytoskeletal components of metazoan cells. We have previously determined a tissue specific expression and assembly properties of all eleven cytoplasmic IFs (IFA-1 - IFA-4, IFB-1, IFB-2, IFC-1, IFC-2, IFD-1, IFD-2, IFP-1) in C. elegans and reported an essential function for four (IFA-1, IFA-2, IFA-3 and IFB-1) of them. In this study we continued the characterisation of the IF proteins in C. elegans by searching for in vivo polymerisation partners of the IFA proteins...
January 11, 2019: Gene
Shuyi Zhou, Yuanliang Yan, Xi Chen, Xiang Wang, Shuangshuang Zeng, Long Qian, Jie Wei, Xue Yang, Yangying Zhou, Zhicheng Gong, Zhijie Xu
BACKGROUND: Phosphoribosylaminoimidazole carboxylase (PAICS), a de novo purine metabolic enzyme, has been identified as an oncogene in several tumor types, including breast cancer and prostate cancer. However, the role of PAICS in human lung adenocarcinoma (LADC) requires further study. METHODS: In this research, the effects of PAICS on the occurrence and prognosis of LADC were evaluated using integrative bioinformatics analyses. RESULTS: By employing the bioinformatics analyses of several public databases, PAICS, which is overexpressed in the LADC tissues, was identified as a potential tumor-promoting gene in LADC biology...
January 11, 2019: Gene
Decheng Ren, Jian Hua Xu, Yan Bi, Zhaofeng Zhang, Rui Zhang, Yuyan Li, Jiaxin Hu, Zhenming Guo, Weibo Niu, Fengping Yang, Weidong Li, Yifeng Xu, Lin He, Tao Yu, Junqing Wu, Xingwang Li, Jing Du, Guang He
OBJECTIVE: Obesity is one of the major health problems strongly influenced by lifestyle, genetic and environmental factors. Previous studies have reported many single-nucleotide polymorphisms (SNPs) are associated with obesity in different races. This study aimed to explore the genetic associations between LEPR, MC4R polymorphisms and overweight/obesity in Chinese Han adolescents. METHODS: 400 adolescents including 222 health controls and 178 overweight/obese adolescents were genotyped and their body compositions were also analyzed in this study...
January 11, 2019: Gene
Yang'ou Wu, Jing Yang, Zexin Ai, Miao Yu, Jia Li, Shengjiao Li
BACKGROUND: Mesenchymal stem cells (MSCs) are multipotent cells that can be widely used in stem cell therapy. However, few studies have revealed the potential mechanisms of the changes in aging MSC. MATERIALS AND METHODS: In this study, microarray data GSE35955 was downloaded from the Gene Expression Omnibus database. Then limma package in R was used to filtrate differentially expressed genes (DEGs), Transcription factors (TFs) were predicted by DCGL package. After predicting TFs, protein-protein interaction (PPI) network and TF-mediated transcriptional regulation network were constructed...
January 11, 2019: Gene
Arseny Dubin, Sylvia Ighem Chi, Åse Emblem, Truls Moum, Steinar D Johansen
Mitochondrial genome organization of sea anemones appears conserved among species and families, and is represented by a single circular DNA molecule of 17 to 21 kb. The mitochondrial gene content corresponds to the same 13 protein components of the oxidative phosphorylation (OxPhos) system as in vertebrates. Hallmarks, however, include a highly reduced tRNA gene repertoire and the presence of autocatalytic group I introns. Here we demonstrate that the mitochondrial genome of the deep-water sea anemone Protanthea simplex deviates significantly from that of other known sea anemones...
January 11, 2019: Gene
Qisang Guo, Ying Xiong, Yu Song, Keqin Hua, Shujun Gao
ARHGAP17 has long been thought to be involved in the maintenance of tight junction and epithelial barrier. Recently, a few Rho GTPase activating proteins (RhoGAPs) have been identified as tumor suppressors in some human cancers. The present study aimed to explore ARHGAP17 expression in cervical cancer and the possible function in tumor progression. ARHGAP17 expression in cervical cancer cell lines was assessed by RT-PCR and Western blotting. ARHGAP17 expression in cervical cancer tissues and normal tissues was assessed by immunohistochemistry...
January 11, 2019: Gene
Caineng Zhong, Yuchang Mai, Hengyuan Gao, Wenbin Zhou, Dongxian Zhou
TPA is considered to be a tumor promoting molecule that induces the expression of COX-2 protein. However, it is contradictory to find that TPA can induce tumor cell apoptosis and exert antitumor activity. Therefore, the role of TPA in tumorigenesis and development has not yet been elucidated. Here we show that TPA can promote the apoptosis of breast cancer cells and increase the ratio of Bax/Bcl-2. It is suggested that TPA may induce apoptosis of breast cancer cells through mitochondrial apoptosis pathway. Further studies showed that TPA could cause mitochondrial dysfunction and trigger mitochondrial apoptotic pathway...
January 11, 2019: Gene
Yun Shao, Xiaoli Zhang, Steve van Nocker, Xiaoqing Gong, Fengwang Ma
Members of the sucrose non-fermenting-1-related protein kinase 2 (SnRK2) family play central roles in the abscisic acid (ABA) signaling pathway and in mediating osmotic stress signaling and tolerance in plants. Previously, 12 full-length coding sequences that belong to SnRK2 gene family were identified and cloned in wild apple species Malus prunifolia. In this study, one of the members, MpSnRK2.10, was overexpressed in Arabidopsis and apple to investigate its potential function in response to drought stress...
January 11, 2019: Gene
P Manikandan, D Ramyachitra, R Nandhini
In living organisms, the MicroRNAs act as an important role by controlling regulatory mechanisms, and likely manipulating the output of numerous protein-coding genes. Several computational databases, algorithms and tools have been developed to discover the miRNA target genes. But, the existing methods obtain poorer results in identification of miRNA target gene. Hence in this research work, integrated prediction scores is used to identify the microRNA target interactions and hybrid fuzzy algorithms are used to make final predictions...
January 11, 2019: Gene
Eva Blondeau-Bidet, Junya Hiroi, Catherine Lorin-Nebel
Ion uptake mechanisms are diverse in fish species, certainly linked to duplication events that have led to the presence of a multitude of paralogous genes. In fish, Na+ uptake involves several ion transporters expressed in different ionocyte subtypes. In the European sea bass Dicentrarchus labrax, several key transporters potentially involved in Na+ uptake have been investigated in seawater (SW) and following a 2 weeks freshwater (FW) acclimation. Using gel electrophoresis, we have shown that the Na+ /H+ -exchanger 3 (nhe3, slc9a3) is expressed in gills and kidney at both salinities...
January 11, 2019: Gene
Hyoim Jeon, Le Minh Thong, Byeongyong Ahn, Hye-Sun Cho, Le Van Chanh Quy, Joori Yum, Kwonho Hong, Jin-Hoi Kim, Hyuk Song, Chankyu Park
Proline-arginine-rich (PR)-39 is neutrophil antimicrobial peptide that has potent antimicrobial activity against a broad spectrum of microorganisms, including bacteria, fungi, and some enveloped viruses as a part of the innate immune system. We analyzed the nucleotide sequence variations of PR-39 exon 4, which is the mature peptide region responsible for antimicrobial activity, from 48 pigs of six breeds using sequence-based typing. The analysis identified four alleles including allele PR-35 with a 12-bp deletion near the N-terminus...
January 11, 2019: Gene
Xiaolong Huang, Huiqing Yan, Lisheng Zhai, Yin Yi
GLABROUS1 is an R2R3 MYB homolog that is essential for the initiation of trichome development. In this study, we used RACE to clone a full-length cDNA from Rosa roxburghii Tratt, termed RrGL1, which was 1013 bp, including an open reading frame of 792 bp that encoded 263 amino acids. In situ hybridization, corresponding with qRT-PCR results, revealed that RrGL1 transcripts were mainly expressed in petiole, leaf, and stem. RrGL1 expression levels at various fruit developmental stages in R. roxburghii were also evaluated...
January 11, 2019: Gene
Corina I Schanzenbach, Benedikt Kirchner, Susanne E Ulbrich, Michael W Pfaffl
Common pregnancy detection methods in dairy cattle have limits in sensitivity and timing. Thus, we aimed at finding new early pregnancy biomarkers in milk. A previous study in milk cells and skim milk suggested the possible use of several miRNAs for this purpose. In order to make the sampling procedure more convenient for daily use and to include the milk fat into analysis, here, a similar study was repeated using whole fresh milk of days 4, 12, 18 and 21 of pregnancy and non-pregnant day-matched controls, respectively...
January 11, 2019: Gene
Mohammad Taheri, Rezvan Noroozi, Arash Dehghan, Golnaz Atri Roozbahani, Mir Davood Omrani, Soudeh Ghafouri-Fard
Prostate cancer (PCa) and benign prostate hyperplasia (BPH) as two prevalent age-related disorders in male have some shared genetic and immunological underlying mechanisms. However, researchers have aimed at identification of specific biomarkers with the ability to differentiate between these disorders. In the present study, we genotyped the rs4073, rs2227306 and rs1126647 single nucleotide polymorphisms within IL-8 gene in 530 individuals including 130 PCa patients, 200 BPH patients and 200 male controls. The rs2227306 alleles and genotypes were distributed equally in the three study groups...
January 11, 2019: Gene
Gulnaz F Korytina, Leysan Z Akhmadishina, Yulia G Aznabaeva, Olga V Kochetova, Naufal Sh Zagidullin, Julia G Kzhyshkowska, Shamil Z Zagidullin, Tatyana V Viktorova
BACKGROUND AND OBJECTIVE: Chronic obstructive pulmonary disease (COPD) is a complex chronic inflammatory disease of the respiratory system affecting primarily distal respiratory pathways and lung parenchyma. This work was designed as a case-control study aimed at investigating the association of the NRF2/KEAP1 signaling system, and antioxidant defense gene polymorphisms with COPD in population from Russia. METHODS: Ten SNPs: NFE2L2 (rs35652124), KEAP1 (rs1048290), MPO (rs2333227), PRNP (rs1799990), PTGR1 (rs2273788), HSPA1A (rs1008438), TXNRD2 (rs1139793), GSR (rs1002149), SIRT2 (rs10410544), and PTGS1 (rs1330344) were genotyped by the real-time polymerase chain reaction (TaqMan assays) in a case-control study (425 COPD patients and 457 controls, from the same region of Russia, representatives of Tatar population)...
January 11, 2019: Gene
Yexiong Qian, Qiaoyu Ren, Jing Zhang, Liang Chen
High temperature is a common stress, which influences the growth and reproduction of plants. Maize is one of the most important crops all over the world. However, heat stress reduces significantly the yield and quality of maize. Therefore, it is important to illuminate molecular mechanism of maize response to heat stress. To estimate genes related to heat stress, we analyzed the transcriptome of maize in response to heat stress. In this study, six cDNA libraries were constructed form total RNA isolated from leaves of maize...
January 11, 2019: Gene
Shahrzad Pouyanrad, Soheila Rahgozar, Elaheh Sadat Ghodousi
Acute lymphoblastic leukemia (ALL) is the most prevalent cancer among children, and multidrug efflux mediated by overexpression of ABC transporters is the major impediment to successful chemotherapy in this malignancy. The goal of this study is to identify the non-coding RNAs (ncRNAs) which may affect the expression levels of ABCA3; the previously identified prognostic biomarker for multidrug resistance (MDR) in childhood ALL (cALL). Bone marrow samples from 64 cALLs, including 46 de novo and 18 relapsed patients, in addition to 30 non-cancer controls were collected, and ncRNAs were nominated using in silico studies...
January 10, 2019: Gene
George P Livi
The quest to elucidate the molecular mechanism of action of rapamycin in the early 1990s led to the discovery of the novel TOR (target of rapamycin) proteins in yeast and mammalian cells. This was a major breakthrough that resulted in the development of new rapamycin analogs as anti-cancer agents, and launched new research that revealed the pre-eminent biological role of mTOR (mammalian or mechanistic TOR). Beyond mediating rapamycin sensitivity, the TOR proteins are nutrient sensing protein kinases, conserved from yeast to man, with a core function in regulating cell growth, metabolism and overall cell survival...
January 9, 2019: Gene
Pranjal Kumar, Seema Khadirnaikar, Sudhanshu Kumar Shukla
Lung Adenocarcinoma (LUAD) is the most common cause of lung cancer-related deaths. Long non-coding RNAs (LncRNAs) play an essential role in cancer development and progression. In this study, we identified PILAR1, a prognostic and overexpressed LncRNA, using multiple independent datasets of LUAD patients. Higher expression of PILAR1 was associated with survival in Dhanasekaran et al. (HR = 2.29, p-value = 0.017), TCGA (HR = 1.51, p-value = 0.017) and KM plotter (HR = 2.67, p-value ≤ 0...
January 9, 2019: Gene
Showkat Ahmad Dar, Prem P Srivastava, Tincy Varghese, Mir Ishfaq Nazir, Subodh Gupta, Gopal Krishna
A short term starvation and refeeding experiment was conducted to study the temporal changes in SOD, CAT and HSP70 gene expression of Labeo rohita fingerlings. The study was carried out for 15 days with initial 7 days of starvation and then refeeding up to 15th day of the experimental trial. The expressions of SOD and CAT genes of liver and gills were significantly up-regulated after 7 days of starvation, down-regulated after 3 days of refeeding, and returned to the basal values after 8 days of refeeding...
January 8, 2019: Gene
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