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https://www.readbyqxmd.com/read/27923672/functional-characterization-of-two-novel-purine-transporters-from-the-basidiomycota-phanerochaete-chrysosporium
#1
Mariana Barraco-Vega, Héctor Romero, Mariana Richero, María Pía Cerdeiras, Gianna Cecchetto
Purine transporters as substrate entry points in organisms, are involved in a number of cellular processes such as nitrogen source uptake, energy metabolism and synthesis of nucleic acids. In this study, two nucleobase transporter genes (phZ, phU) from Phanerochaete chrysosporium were cloned, identified, and functionally characterized. Our results show that PhZ is a transporter of adenine and hypoxanthine, and a protein belonging to the AzgA-like family, whilst PhU belongs to the NAT/NCS2 family, transporting xanthine and uric acid...
December 3, 2016: Gene
https://www.readbyqxmd.com/read/27923671/a-core-component-of-the-cul4-ubiquitin-ligase-complexes-ddb1-regulates-spermatogenesis-in-the-chinese-mitten-crab-eriocheir-sinensis
#2
AnYu Fang, XueJie Li, YuanLi Wang, DiYue Pan, Qun Wang
Studies in mammals have shown that damaged DNA-binding protein 1 (DDB1) is a multifunctional protein that recognizes UV-induced DNA lesions and activates nucleotide excision repair process, and could also be a linker protein for Cullin4 in ubiquitination to regulate cell cycle progression. However, there are few studies of DBB1 in crustaceans. In this study, a cDNA representing the DDB1 gene from Eriocheir sinensis (Es-DDB1) was cloned successfully. The full length Es-DDB1 cDNA comprises 4871 nucleotides, and encodes an open-reading frame (ORF) of 1137 amino acid residues...
December 3, 2016: Gene
https://www.readbyqxmd.com/read/27919705/the-complete-mitochondrial-genome-of-thitarodes-sejilaensis-lepidoptera-hepialidae-a-host-insect-of-ophiocordyceps-sinensis-and-its-implication-in-taxonomic-revision-of-hepialusadopted-in-china
#3
Zhiwen Zou, Qiang Min, Shiyu Cheng, Tianrong Xin, Bin Xia
The mitochondrial genome is widely used for phylogenetic analyses and evolutionary biology. The complete mitochondrial genome of Thitarodes sejilaensis (Lepidoptera: Hepialidae) was sequenced and analyzed in this study. This mitogenome is a typical circular molecule of 15,290bp, with the gene content, orientation and order identical to other insects in the family Hepialidae. The genome nucleotide composition is heavily biased towards As and Ts, accounting for 80.87% of total nucleotide content. The major strand shows a positive AT-skew and negative GC-skew...
December 2, 2016: Gene
https://www.readbyqxmd.com/read/27919704/global-transcriptome-analysis-of-halolamina-sp-to-decipher-the-salt-tolerance-in-extremely-halophilic-archaea
#4
Aslıhan Kurt-Kızıldoğan, Büşra Abanoz, Sezer Okay
Extremely halophilic archaea survive in the hypersaline environments such as salt lakes or salt mines. Therefore, these microorganisms are good sources to investigate the molecular mechanisms underlying the tolerance to high salt concentrations. In this study, a global transcriptome analysis was conducted in an extremely halophilic archaeon, Halolamina sp. YKT1, isolated from a salt mine in Turkey. A comparative RNA-seq analysis was performed using YKT1 isolate grown either at 2.7M NaCl or 5.5M NaCl concentrations...
December 2, 2016: Gene
https://www.readbyqxmd.com/read/27916718/loss-of-the-opa-interacting-protein-5-inhibits-breast-cancer-proliferation-through-mir-139-5p-notch1-pathway
#5
Hong-Chang Li, Ya-Feng Chen, Wen Feng, Han Cai, Yi Mei, Yi-Ming Jiang, Teng Chen, Ke Xu, Dian-Xu Feng
Opa interacting protein 5 (OIP5) has been reported to be over-expressed in several kinds of human cancer. However, the biological function and clinical significance of OIP5 in human breast cancer remains unknown. In this study, we found that OIP5 was notably over-expressed in breast cancer tissues compared with their corresponding nontumorous tissues. Statistical analysis showed a significant correlation of OIP5 expression with advanced clinical stage. Ablation OIP5 inhibited the proliferation of breast cancer cells...
December 1, 2016: Gene
https://www.readbyqxmd.com/read/27916717/is-luks-pv-a-novel-experimental-therapy-for-leukemia
#6
REVIEW
Wulin Shan, Xiaoling Ma, Fang Deng
Although the studies on the pathogenesis and prognosis of leukemia have made revolutionary progress, the long-term survival remains unsatisfactory. Alternative techniques are being developed to target leukemia. Several decades after researchers' work, a variety of bacteria toxins are being explored as potential anti-leukemia agents, either to provide direct effects or to deliver therapeutic proteins to leukemia. LukS-PV, a component of Panton-Valentine Leukocidin secreted by S. aureus, has been tested in acute myeloid leukemia as a novel experimental strategy...
December 1, 2016: Gene
https://www.readbyqxmd.com/read/27914980/cloning-and-characterization-of-adipogenin-and-its-overexpression-enhances-fat-accumulation-of-bovine-myosatellite-cells
#7
Yang Liu, Bijie Jiang, Changzhen Fu, Ruijie Hao
Adipogenin (ADIG) is an adipocyte-specific membrane protein highly expressed in adipose tissues and is increased during the adipocyte differentiation. However, the roles and mechanisms of ADIG on fat accumulation and adipocyte differentiation in ex vivo still largely unknown. In this study, we isolated bovine myosatellite cells based on adhesion characteristics to investigate whether ADIG overexpression could promote trans-differentiation and increase fat accumulation in myosatellite cells. Immunofluorescence labeling was then used for the phenotypic characteristics of myosatellite...
November 30, 2016: Gene
https://www.readbyqxmd.com/read/27913185/mir-346-promotes-the-biological-function-of-breast-cancer-cells-by-targeting-srcin1-and-reduces-chemosensitivity-to-docetaxel
#8
Fan Yang, Long-Ji Luo, Lei Zhang, Dan-Dan Wang, Su-Jin Yang, Li Ding, Jian Li, Dan Chen, Rong Ma, Jian-Zhong Wu, Jin-Hai Tang
MicroRNAs (miRNAs) are a class of highly conserved small noncoding RNAs that play pivotal roles at the post-transcriptional level in the biological function of various cancers, including breast cancer. In our study, miR-346 mimic, inhibitor, negative control or si-SRCIN1 were transfected into MCF-7 and MCF-7/Doc cells, respectively. Quantitative real time PCR (qRT-PCR) was used to measure miR-346 and SRCIN1 mRNA expressions and western blot was used to detect the expression of SRCIN1 in protein level. CCK-8 and colony formation were employed to verify cell viability and proliferation...
November 29, 2016: Gene
https://www.readbyqxmd.com/read/27899326/complete-plastid-genome-sequence-of-goosegrass-eleusine-indica-and-comparison-with-other-poaceae
#9
Hui Zhang, Nathan Hall, J Scott McElroy, Elijah K Lowe, Leslie R Goertzen
Eleusine indica, also known as goosegrass, is a serious weed in at least 42 countries. In this paper we report the complete plastid genome sequence of goosegrass obtained by de novo assembly of paired-end and mate-paired reads generated by Illumina sequencing of total genomic DNA. The goosegrass plastome is a circular molecule of 135,151bp in length, consisting of two single-copy regions separated by a pair of inverted repeats (IRs) of 20,919 bases. The large (LSC) and the small (SSC) single-copy regions span 80,667 bases and 12,646 bases, respectively...
November 26, 2016: Gene
https://www.readbyqxmd.com/read/27889371/identification-and-molecular-characterization-of-escherichia-coli-blashv-genes-in-a-chinese-teaching-hospital
#10
Mei Zhu, Guangjian Yang, Ailing Li, Li Zong, Zhaoguang Dong, Junwan Lu, Kaibo Zhang, Cong Cheng, Qingli Chang, Xiuying Wu, Jianchao Ying, Xianneng Li, Li Ding, Haixiao Zheng, Junping Yu, Jun Ying, Teng Xu, Huiguang Yi, Peizhen Li, Kewei Li, Songquan Wu, Qiyu Bao, Junrong Wang
Escherichia coli (E. coli) commonly reside in human intestine and most E. coli strains are harmless, but some serotypes cause serious food poisoning. This study identified and molecularly characterized E. coli blaSHV genes from 490 E. coli strains with multi-drug resistance in a hospital population. PCR and molecular cloning and southern blot were performed to assess functions and localizations of this resistant E. coli gene and the pulsed-field gel electrophoresis (PFGE) was utilized to demonstrate the clonal relatedness of the positive E...
November 23, 2016: Gene
https://www.readbyqxmd.com/read/27884772/ovol2-gene-inhibits-the-epithelial-to-mesenchymal-transition-in-lung-adenocarcinoma-by-transcriptionally-repressing-twist1
#11
Zhi-Hai Wang, Zhong Li, Meng Hu, Qing-Jie Yang, Shen Yan, Rong-Si Wu, Bo-An Li, Ming Guo
BACKGROUND: Associated with recent achievements in therapy for advanced lung adenocarcinoma, there will still be an unmet medical need for effective treatment of stage IIIb/IV, and the prognosis of lung cancer is not optimistic till now. OBJECTIVE: In order to obtain some essential evidences for a potential targeted therapy in lung adenocarcinoma, the effects of Ovol2 gene on Epithelial-to-Mesenchymal Transition (EMT) was observed and the probable mechanisms were analyzed...
November 21, 2016: Gene
https://www.readbyqxmd.com/read/27876533/multiple-single-nucleotide-polymorphisms-in-the-first-intron-of-the-il2ra-gene-affect-transcription-factor-binding-and-enhancer-activity
#12
Anton M Schwartz, Denis E Demin, Ilya E Vorontsov, Artem S Kasyanov, Lidia V Putlyaeva, Karina A Tatosyan, Ivan V Kulakovskiy, Dmitry V Kuprash
IL2RA gene encodes the alpha subunit of a high-affinity receptor for interleukin-2 which is expressed by several distinct populations of lymphocytes involved in autoimmune processes. A large number of polymorphic alleles of the IL2RA locus are associated with the development of various autoimmune diseases. With bioinformatics analysis we the dissected the first intron of the IL2RA gene and selected several single nucleotide polymorphisms (SNPs) that may influence the regulation of the IL2RA gene in cell types relevant to autoimmune pathology...
November 19, 2016: Gene
https://www.readbyqxmd.com/read/27871926/the-telomere-attrition-rate-is-not-accelerated-in-women-born-small-for-gestational-age-a-birth-cohort-study
#13
Anderson Sanches de Melo, Rosana Maria Dos Reis, Rodrigo do Tocantins Calado de Saloma Rodrigues, Ricardo de Carvalho Cavalli, Heloisa Bettiol, Viviane Cunha Cardoso, Rui Alberto Ferriani, Marco Antonio Barbieri, Carolina Sales Vieira
Physiologically, a reduction in telomere length (LTL) occurs with aging, but epigenetic changes may accelerate telomere shortening and also facilitate the onset of oxidative/inflammatory stress and the development of clinical/metabolic comorbidities in life spam. Although individuals born small for gestational age (SGA) may be related to those epigenetic changes, the assessment of LTL in individuals born SGA has yielded conflicting results (only cross-sectional studies) and has not been carried out in longitudinal studies...
November 18, 2016: Gene
https://www.readbyqxmd.com/read/27871925/identification-and-characterization-of-a-phospholipid-scramblase-encoded-by-planarian-dugesia-japonica
#14
Yu Han, Ao Li, Lili Gao, Weiwei Wu, Hongkuan Deng, Wenjing Hu, Na Li, Shimin Sun, Xiufang Zhang, Bosheng Zhao, Baohua Liu, Qiuxiang Pang
Phospholipid scramblases (PLSCRs) are the conserved calcium-binding, type II transmembrane proteins synthesized in all eukaryotic organisms. In mammals, these proteins play essential roles in various physiological processes, especially in the immune responses. However, the existence of PLSCRs and their biological functions in planarian are still unknown at present. In this study, a new member of PLSCRs was identified in planarian Dugesia japonica (D. japonica), named DjPLSCR. The sequence analysis revealed that it contains an opening reading frame consisting of 897bp encoding a putative protein of 241 amino acids with a predicted molecular mass of ~28...
November 18, 2016: Gene
https://www.readbyqxmd.com/read/27871924/mir-335-regulates-the-chemo-radioresistance-of-small-cell-lung-cancer-cells-by-targeting-parp-1
#15
Yingshan Luo, Lihua Tong, Hui Meng, Weiliang Zhu, Linlang Guo, Ting Wei, Jian Zhang
The role of miR-335 in the regulation of chemosensitivity and radiosensitivity of small cell lung cancer (SCLC) was investigated. miR-335 was significantly downregulated in multi-drug-resistant SCLC H69AR and H446DDP cells compared with parental cells as detected by qRT-PCR. Then, we demonstrated the negative correlation between miR-335 expression and the chemo-radiosensitivity of SCLC cells, including cell proliferation, cell clonality and cell apoptosis. In addition, miR-335 overexpression inhibited cell migration in vitro and tumor growth in vivo, whereas inhibition of miR-335 promoted cell migration and tumor growth...
November 18, 2016: Gene
https://www.readbyqxmd.com/read/27871923/omics-analysis-of-mouse-brain-models-of-human-diseases
#16
Véronique Paban, Béatrice Loriod, Claude Villard, Luc Buee, David Blum, Susanna Pietropaolo, Yoon H Cho, Sylvie Gory-Faure, Elodie Mansour, Ali Gharbi, Béatrice Alescio-Lautier
The identification of common gene/protein profiles related to brain alterations, if they exist, may indicate the convergence of the pathogenic mechanisms driving brain disorders. Six genetically engineered mouse lines modelling neurodegenerative diseases and neuropsychiatric disorders were considered. Omics approaches, including transcriptomic and proteomic methods, were used. The gene/protein lists were used for inter-disease comparisons and further functional and network investigations. When the inter-disease comparison was performed using the gene symbol identifiers, the number of genes/proteins involved in multiple diseases decreased rapidly...
November 18, 2016: Gene
https://www.readbyqxmd.com/read/27866042/carcinogenic-effects-of-oil-dispersants-a-kegg-pathway-based-rna-seq-study-of-human-airway-epithelial-cells
#17
Yao-Zhong Liu, Lei Zhang, Astrid M Roy-Engel, Shigeki Saito, Joseph A Lasky, Guangdi Wang, He Wang
The health impacts of the BP oil spill are yet to be further revealed as the toxicological effects of oil products and dispersants on human respiratory system may be latent and complex, and hence difficult to study and follow up. Here we performed RNA-seq analyses of a system of human airway epithelial cells treated with the BP crude oil and/or dispersants Corexit 9500 and Corexit 9527 that were used to help break up the oil spill. Based on the RNA-seq data, we then systemically analyzed the transcriptomic perturbations of the cells at the KEGG pathway level using two pathway-based analysis tools, GAGE (generally applicable gene set enrichment) and GSNCA (Gene Sets Net Correlations Analysis)...
November 16, 2016: Gene
https://www.readbyqxmd.com/read/27864010/differentially-expressed-genes-in-the-testicular-tissues-of-adenylyl-cyclase-3-knockout-mice
#18
Jing Zhang, Yuan Yuan, Qingxiu Liu, Dong Yang, Mingshen Liu, Limin Shen, Yanfen Zhou, Zhenshan Wang
Adenylate cyclase 3 (AC3) is an important component of the cyclic adenosine 3',5'-monophosphate (cAMP) signaling pathway and converts adenosine triphosphate into cAMP. Male mice with AC3 deletion (AC3(-/-)) are sterile. However, the mechanical mechanism remains unclear. By TUNEL staining, we found that cell apoptosis in the testicular tissues of AC3(-/-) mice increased significantly compared with that in the wild-type (AC3(+/+)) mice. Differentially expressed genes regulated by AC3 in the testicular tissues were identified by gene chip hybridization...
November 15, 2016: Gene
https://www.readbyqxmd.com/read/27864009/evolutionary-dynamics-of-triosephosphate-isomerase-gene-intron-location-pattern-in-metazoa-a-new-perspective-on-intron-evolution-in-animals
#19
Bing Chen, Jingru Shao, Huifu Zhuang, Jianfan Wen
Intron evolution, including its dynamics in the evolutionary transitions and diversification of eukaryotes, remains elusive. Inadequate taxon sampling due to data shortage, unclear phylogenetic framework, and inappropriate outgroup application might be among the causes. Besides, the integrity of all the introns within a gene was often neglected previously. Taking advantage of the ancient conserved triosephosphate isomerase gene (tim), the relatively robust phylogeny of Metazoa, and choanoflagellates as outgroup, the evolutionary dynamics of tim intron location pattern (ILP) in Metazoa was investigated...
November 15, 2016: Gene
https://www.readbyqxmd.com/read/27851981/panget-pan-genomics-tool
#20
Iyyappan Yuvaraj, Jayavel Sridhar, Daliah Michael, Kanagaraj Sekar
A decade after the concept of Pan-genome was first introduced; research in this field has spread its tentacles to areas such as pathogenesis of diseases, bacterial evolutionary studies and drug resistance. Gene content-based differentiation of virulent and a virulent strains of bacteria and identification of pathogen specific genes is imperative to understand their physiology and gain insights into the mechanism of genome evolution. Subsequently, this will aid in identifying diagnostic targets and in developing and selecting vaccines...
November 13, 2016: Gene
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