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https://www.readbyqxmd.com/read/28302418/lncrna-atb-microrna-200a-%C3%AE-catenin-regulatory-axis-involved-in-the-progression-of-hcv-related-hepatic-fibrosis
#1
Na Fu, Su-Xian Zhao, Ling-Bo Kong, Jing-Hua Du, Wei-Guang Ren, Fang Han, Qing-Shan Zhang, Wen-Cong Li, Cui Po, Rong-Qi Wang, Yu-Guo Zhang, Yue-Min Nan
OBJECTIVE(S): Long noncoding RNAs (lncRNAs)-activated by transforming growth factor beta (lncRNA-ATB) is known to be involved in the invasion of hepatocellular carcinoma by regulating target gene miR-200a. However, the role and molecular mechanisms of lncRNA-ATB/miR-200a in HCV-related liver fibrosis remains unclear. In this study, we examined the expression of lncRNA-ATB/miR-200a, and their target gene β-Catenin in liver tissues of HCV patients and hepatic stellate cells (HSCs) to elucidate the possible role of lncRNA-ATB/miR-200a axis in HSC activation and development of liver fibrosis...
March 13, 2017: Gene
https://www.readbyqxmd.com/read/28300613/identification-and-characterization-of-a-masculinizer-masc-gene-involved-in-sex-differentiation-in-artemia
#2
Dong-Rui Li, Hui-Li Ye, Jin-Shu Yang, Fan Yang, Mo-Ran Wang, Stephanie De Vos, Marnik Vuylsteke, Patrick Sorgeloos, Gilbert Van Stappen, Peter Bossier, Wei-Jun Yang
The sex of relatively primitive animals such as invertebrates is mostly determined by environmental factors and chromosome ploidy. Heteromorphic chromosomes may also play an important role, as in the ZW system in lepidopterans. However, the mechanisms of these various sex determination systems are still largely undefined. In the present study, a Masculinizer gene (Ar-Masc) was identified in the crustacean Artemia franciscana Kellogg 1906. Sequence analysis revealed that the 1140-bp full-length open reading frame of Ar-Masc encodes a 380-aa protein containing two CCCH-type zinc finger domains having a high degree of shared identities with the MASC protein characterized in the silkworm Bombyx mori, which has been determined to participate in the production of male-specific splice variants...
March 11, 2017: Gene
https://www.readbyqxmd.com/read/28300612/characterization-of-melanin-concentrating-hormone-mch-and-its-receptor-in-chickens-tissue-expression-functional-analysis-and-fasting-induced-up-regulation-of-hypothalamic-mch-expression
#3
Lin Cui, Chan Lv, Jiannan Zhang, Chunheng Mo, Dongliang Lin, Juan Li, Yajun Wang
Melanin-concentrating hormone (MCH) is a neuropeptide expressed in the brain and exerts its actions through interaction with the two known G protein-coupled receptors, namely melanin-concentrating hormone receptor 1 and 2 (MCHR1 and MCHR2) in mammals. However, the information regarding the expression and functionality of MCH and MCHR(s) remains largely unknown in birds. In this study, using RT-PCR and RACE PCR, we amplified and cloned a MCHR1-like receptor, which is named cMCHR4 according to its evolutionary origin, and a MCHR2 from chicken brain...
March 11, 2017: Gene
https://www.readbyqxmd.com/read/28284878/differential-expression-of-krt83-regulated-by-the-transcript-factor-cap1-in-chinese-tan-sheep
#4
Yufang Liu, Xiaolong Kang, Wanjie Yang, Minggui Xie, Jibin Zhang, Meiying Fang
Keratin 83 (KRT83) is an important keratin protein in hair development. In this study, expression of KRT83 was compared among different tissues and between 1-month-old lambs and 48-month adult of Chinese Tan sheep, which showed different fleece phenotypes. The results showed that KRT83 was only expressed in skin, and KRT83 mRNA level in skin was significantly higher in Tan lambs than in adult sheep. To further understand the expression regulation of KRT83 by transcription factors in Tan sheep, amplified sequences coving different ranges of KRT83 promoter region were inserted into a pGL3-basic vector and then transfected into sheep primary fibroblast cells...
March 8, 2017: Gene
https://www.readbyqxmd.com/read/28286087/minos-insertion-mutant-of-the-drosophila-gba-gene-homologue-showed-abnormal-phenotypes-of-climbing-ability-sleep-and-life-span-with-accumulation-of-hydroxy-glucocerebroside
#5
Haruhisa Kawasaki, Takahiro Suzuki, Kumpei Ito, Tsubasa Takahara, Naoko Goto-Inoue, Mitsutoshi Setou, Kazuki Sakata, Norio Ishida
Gaucher's disease in humans is considered a deficiency of glucocerebrosidase (GlcCerase) that result in the accumulation of its substrate, glucocerebroside (GlcCer). Although mouse models of Gaucher's disease have been reported from several laboratories, these models are limited due to the perinatal lethality of GlcCerase gene. Here, we examined phenotypes of Drosophila melanogaster homologues genes of the human Gaucher's disease gene by using Minos insertion. One of two Minos insertion mutants to unknown function gene (CG31414) accumulates the hydroxy-GlcCer in whole body of Drosophila melanogaster...
March 7, 2017: Gene
https://www.readbyqxmd.com/read/28286086/hyper-methylation-of-the-upstream-cpg-island-shore-is-a-likely-mechanism-of-gper1-silencing-in-breast-cancer-cells
#6
Mohan C Manjegowda, Paridhi Singhal Gupta, Anil M Limaye
GPER1, also known as GPR30, is a novel seven-transmembrane G-protein coupled estrogen receptor that mediates both short-term (non-genomic) and long-term (genomic) effects of estrogen in target cells and tissues. A substantial body of work over the last two decades has highlighted its therapeutic or prognostic utility. However, the clinical data on the expression of GPER1 in breast tissue is ambiguous. Analysis of TCGA RNAseq data revealed significantly lower mean expression of GPER1 mRNA in primary breast tumors compared to that in normal breast tissues...
March 7, 2017: Gene
https://www.readbyqxmd.com/read/28286085/hspa5-gene-encoding-hsp70-chaperone-bip-in-the-endoplasmic-reticulum
#7
REVIEW
Jie Wang, Jessica Lee, David Liem, Peipei Ping
The HSPA5 gene (Ensembl ID: ENSG00000044574 (WTSI/EMBL-EBI, 2015)) encodes the binding immunoglobulin protein (BiP), an Hsp70 family chaperone localized in the ER lumen. As a highly conserved molecular chaperone, BiP assists in a wide range of folding processes via its two structural domains, a nucleotide-binding domain (NBD) and substrate-binding domain (SBD). BiP is also an essential component of the translocation machinery for protein import into the ER, a regulator for Ca(2+) homeostasis in the ER, as well as a facilitator of ER-associated protein degradation (ERAD) via retrograde transportation of aberrant proteins across the ER membrane...
March 7, 2017: Gene
https://www.readbyqxmd.com/read/28268179/vibrio-parahaemolyticus-calr-down-regulates-the-thermostable-direct-hemolysin-tdh-gene-transcription-and-thereby-inhibits-hemolytic-activity
#8
Yiquan Zhang, Ying Zhang, He Gao, Lingyu Zhang, Zhe Yin, Xinxiang Huang, Dongsheng Zhou, Huiying Yang, Wenhui Yang, Li Wang
TDH, encoded by tdh gene, is a major virulent determinant of V. parahaemolyticus that controls various biological activities, such as hemolytic activity, cytotoxicity, and enterotoxicity. The hemolytic activity on Wagatsuma agar ascribed to TDH is called Kanagawa phenomenon (KP). All KP positive strains contain tdh1 and tdh2 genes, but tdh2 is predominantly responsible for KP. CalR is a regulatory protein that was originally identified as a repressor of swarming motility and T3SS1 gene expression in V. parahaemolyticus...
March 4, 2017: Gene
https://www.readbyqxmd.com/read/28267575/pdgfs-and-their-receptors
#9
REVIEW
Andrius Kazlauskas
The platelet-derived growth factor (PDGF)/PDGFR receptor (PDGFR) family is essential for a vast array of physiological processes such as migration and proliferation of percityes that contribute to the formation and proper function of blood vessels. While ligand-dependent de-repression of the PDGFR's kinase activity is the major mode by which the PDGFR is activated, there are additional mechanisms to activate PDGFRs. Deregulated PDGFR activity contributes to various pathological conditions, and hence the PDGF/PDGFR family members are viable therapeutic targets...
March 3, 2017: Gene
https://www.readbyqxmd.com/read/28263861/proximity-of-radiation-desiccation-response-motif-to-the-core-promoter-is-essential-for-basal-repression-as-well-as-gamma-radiation-induced-gyrb-gene-expression-in-deinococcus-radiodurans
#10
Narasimha Anaganti, Bhakti Basu, Rita Mukhopadhyaya, Shree Kumar Apte
The radioresistant D. radiodurans regulates its DNA damage regulon (DDR) through interaction between a 17bp palindromic cis-regulatory element called the Radiation Desiccation Response Motif (RDRM), the DdrO repressor and a protease IrrE. The role of RDRM in regulation of DDR was dissected by constructing RDRM sequence-, position- or deletion-variants of Deinococcal gyrB gene (DR0906) promoter and by RDRM insertion in the non-RDRM groESL gene (DR0606) promoter, and monitoring the effect of such modifications on the basal as well as gamma radiation inducible promoter activity by quantifying fluorescence of a GFP reporter...
March 2, 2017: Gene
https://www.readbyqxmd.com/read/28263860/subcellular-distribution-of-the-18kda-translocator-protein-and-transcript-variant-pbr-s-in-human-cells
#11
Guo-Jun Liu, Ryan J Middleton, Richard B Banati
Despite continued interest in the 18kDa translocator protein (PBR/TSPO) as a biomarker and a therapeutic target for a range of diseases, its functional role, such as in the steroid synthesis pathway and energy metabolism has either become contentious or remains to be described more precisely. The PBR/TSPO gene consists of four exons, while a shorter isoform termed PBR-S lacks exon 2. The PBR-S 102-codon open reading frame differs to that of PBR/TSPO, resulting in a protein that is completely unrelated to PBR/TSPO...
March 2, 2017: Gene
https://www.readbyqxmd.com/read/28259691/hotair-genetic-variants-are-associated-with-prostate-cancer-and-benign-prostate-hyperplasia-in-an-iranian-population
#12
Mohammad Taheri, Mohsen Habibi, Rezvan Noroozi, Azadeh Rakhshan, Shaghayegh Sarrafzadeh, Arezou Sayad, Mir Davood Omrani, Soudeh Ghafouri-Fard
Prostate cancer and benign prostate hyperplasia (BPH) are heterogeneous disorders with a wide array of clinical presentations and high prevalence among men. Several protein coding genes as well as non-coding genes have been shown to contribute in prostate cancer and BPH risk. Among non-coding genes whose contribution in tumorigenesis has been identified is HOX transcript antisense RNA (HOTAIR). In the present study we aimed at identification of the associations between three HOTAIR polymorphisms (rs12826786, rs1899663 and rs4759314) and risk of prostate cancer and BPH by the means of tetra-primer ARMS-PCR in a population of 128 Iranian prostate cancer patients, 143 BPH patients and 250 normal male controls...
March 1, 2017: Gene
https://www.readbyqxmd.com/read/28259690/the-herbal-medicine-melissa-officinalis-extract-effects-on-gene-expression-of-p53-bcl-2-her2-vegf-a-and-htert-in-human-lung-breast-and-prostate-cancer-cell-lines
#13
Rana Jahanban-Esfahlan, Khaled Seidi, Amir Monfaredan, Vahid Shafie-Irannejad, Mehran Mesgari Abbasi, Ansar Karimian, Bahman Yousefi
INTRODUCTION: Earlier, we verified that Melissa officinalis extract (MOE) elicits potent antiproliferative effects on different human cancer cells. To gain insights into the molecular mechanisms accounting for the cytotoxic effects of MOE, we assessed the expression patterns of several prominent molecules with therapeutic potential in cancer by Quantitative PCR (Q-PCR). METHODS: A549, MCF-7 and PC3 cancer cells were grown in complete RPMI 1640 and seeded in 24 well micro plates...
March 1, 2017: Gene
https://www.readbyqxmd.com/read/28257836/association-analysis-of-fto-gene-polymorphisms-with-obesity-in-greek-adults
#14
Yiannis Goutzelas, Kalliopi Kotsa, Yiannis Vasilopoulos, Xanthippi Tsekmekidou, Costas Stamatis, John G Yovos, Theologia Sarafidou, Zissis Mamuris
Nowadays, obesity is the greatest scourge worldwide, particularly for the developed countries and is a huge burden for the public health. Over the past decade, GWAS have revealed a number of genes associated with obesity. The fat mass and obesity associated (FTO) gene was the first one associated with obesity in a significant number of populations and recent meta-analysis studies confirm this association. FTO is a N-methyladenosine demethylase and in addition to the genetic association, its biological role in the regulation of body weight has been documented...
February 28, 2017: Gene
https://www.readbyqxmd.com/read/28257835/stratifying-melanoma-and-breast-cancer-tcga-datasets-on-the-basis-of-the-cnv-of-transcription-factor-binding-sites-common-to-proliferation-and-apoptosis-effector-genes
#15
James A Mauro, John M Yavorski, George Blanck
Transcription factors that activate both proliferation- and apoptosis-effector genes, along with a number of related observations, have led to a proposal for a feed forward mechanism of activating the two gene classes, whereby a certain concentration of a transcription factor activates the proliferation-effector genes and a higher concentration of the transcription factor activates the apoptosis-effector genes. We reasoned that this paradigm of regulation could lead to, in the cancer setting, a selection for relatively reduced copy numbers of apoptosis-effector gene, transcription factor binding sites (TFBS)...
February 28, 2017: Gene
https://www.readbyqxmd.com/read/28257834/anxa1ac2-26-peptide-a-possible-therapeutic-approach-in-inflammatory-ocular-diseases
#16
Laila Toniol Cardin, Nathália Martins Sonehara, Kallyne Kioko Oliveira Mimura, Anemari Ramos Dinarte Dos Santos, Wilson Araújo da Silva, Lays Martin Sobral, Andréia Machado Leopoldino, Bianca Rodrigues da Cunha, Eloiza H Tajara, Sonia Maria Oliani, Flávia Cristina Rodrigues-Lisoni
The eye is immunologically privileged when inflammatory responses are suppressed. One component responsible for the suppression of inflammatory responses is the blood retinal barrier, which comprises the retinal pigment epithelium. The destruction of this barrier initiates inflammation, which can affect any part of the eye. Therefore, inflammatory response is controlled by the action of anti-inflammatory mediators, among these mediators, annexin A1 (ANXA1) protein acts as a modulator of inflammation. In this study we aimed to improve the knowledge of this area by investigating how a peptide of the ANXA1 protein (ANXA1Ac2-26) modulates the morphology, proliferation, migration and expression of genes and proteins in human retinal pigment epithelium cells (ARPE-19)...
February 28, 2017: Gene
https://www.readbyqxmd.com/read/28254634/competitive-regulation-of-ipo4-transcription-by-elk1-and-gabp
#17
Pengpeng Xu, Wenbo Lin, Fenglin Liu, Alan Tartakoff, Tao Tao
Nuclear import is a highly selective process that involves the specific recognition of appropriate import signals by suitable receptors. Many nuclear transport pathways are mediated by importin β superfamily members. Among them, IPO4 is a nuclear import receptor for many cargoes. However, its transcriptional regulation remains largely unknown. In the present study, we identified a core region encompassing nt -118 to +108 that is necessary for its promoter activity. Transcription factors binding to this region were screened, resulting in the identification of two members of the Ets family, Ets-like transcription factor-1 and GA binding protein, which repress or activate its promoter activity, respectively...
February 27, 2017: Gene
https://www.readbyqxmd.com/read/28249773/toad-toxin-resistant-snake-thamnophis-elegans-expresses-high-levels-of-mutant-na-k-atpase-mrna-in-cardiac-muscle
#18
Shabnam Mohammadi, Alan H Savitzky, Jennifer Lohr, Susanne Dobler
Toads are chemically defended by bufadienolides, which are lethal to most predators. These toxins exert their lethal effects by binding to and disabling the Na(+)/K(+)-ATPases of cell membranes. Many species of snakes exhibit resistance to the effects of bufadienolides due to target-site insensitivity of the Na(+)/K(+)-ATPase. Mutations that confer resistance have previously been identified in ATP1a3, the gene that codes for the Na(+)/K(+)-ATPase α-3 paralog. We have found that this mutant gene is expressed at a significantly elevated level in heart tissue compared to gut, kidney, and liver of the bufadienolide-resistant snake, Thamnophis elegans...
February 26, 2017: Gene
https://www.readbyqxmd.com/read/28249772/the-association-between-opg-rs3102735-gene-polymorphism-microembolic-signal-and-stroke-severity-in-acute-ischemic-stroke-patients
#19
Hongqin Zhao, Yanyan Cao, Honghua Chen, Wanqun Xu, Ximei Sun, Xudong Pan
BACKGROUND AND PURPOSE: Osteoprotegerin (OPG) is a member of the tumor necrosis factor receptor superfamily and is involved in the progress of atherosclerosis. We chose a gene polymorphism locus, OPG rs3102735, to explore how OPG gene polymorphisms relate to the occurrence of ischemic stroke and microembolic signals and to evaluate their relationship with the severity of neurologic deficits at admission and the degree of vascular stenosis. METHODS: We studied 251 patients diagnosed with large artery atherosclerosis (LAA) stroke and 121 controls...
February 26, 2017: Gene
https://www.readbyqxmd.com/read/28249771/effects-of-the-copy-number-of-ribosomal-genes-genes-for-rrna-on-viability-of-subjects-with-chromosomal-abnormalities
#20
N A Lyapunova, L N Porokhovnik, N V Kosyakova, I A Mandron, T G Tsvetkova
The number of active ribosomal genes (AcRG) was evaluated in 172 carriers of chromosomal abnormalities (CA) such as Down's syndrome (DS), Robertsonian translocations (RT), Klinefelter's and Turner's syndromes, trisomy Х, disomy Y, and various structural CA. In controls (n=318), AcRG dosage varied from 119 to 190 copies with a mean of 151 copies per diploid genome. In CA carriers, except for DS newborns, AcRG dosage was not beyond these limits. As shown previously, only within these limits cellular homeostasis and organism's viability can be supported, while genomes beyond these limits are eliminated by embryonic loss...
February 26, 2017: Gene
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