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Journal of Medical Virology

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https://www.readbyqxmd.com/read/29168898/association-of-p16-cdkn2a-polymorphisms-with-the-development-of-hpv16-related-precancerous-lesions-and-cervical-cancer-in-the-greek-population
#1
D Tsakogiannis, G D Moschonas, E Bella, Z Kyriakopoulou, G D Amoutzias, T G Dimitriou, C Kottaridi, P Markoulatos
The tumor suppressor protein p16 plays a fundamental role in cell cycle regulation and exerts a protective effect against tumor growth. Two different polymorphisms at positions 540 and 580 at the 3'UTR of exon 3 of p16 gene are implicated in several types of cancer, while their role in cervical cancer development remains rather vague. In the present study, we investigated for the impact of p16 genotypes/haplotypes on patients' vulnerability to cervical disease and examined whether these factors can be used as progression markers in the Greek population...
November 23, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29168889/acute-viral-infections-of-the-central-nervous-system-2014-2016-greece
#2
Anna Papa, Elpida Papadopoulou
In order to investigate the viral etiology of acute infections of central nervous system (CNS), multiplex and single PCRs combined with serology for arboviruses were applied on samples from 132 hospitalized patients in Greece during May 2014 to December 2016. A viral pathogen was detected in 52 of 132 (39.4%) cases with acute CNS infection. Enteroviruses predominated (15/52, 28.8%), followed by West Nile virus (9/52, 17.3%). Phleboviruses, varicella-zoster virus and Epstein-Barr virus accounted for 15.4%, 13...
November 23, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29168888/apparent-seronegative-maternal-shingles-with-post-natal-mother-to-baby-transmission-of-varicella-zoster-virus
#3
Lin Pan, Nitesh Singh, Ruth Cannon, Joe Fawke, Julian W Tang
We present an unusual case of varicella zoster (VZ) virus IgG negative, yet clinically apparent, maternal shingles, which prompted the administration of VZ immunoglobulin to the newborn. The mother had no previous VZ vaccination. Eleven days later the baby developed a primary VZ infection, with only mild disease, likely as a result of the VZ immunoglobulin. The variable sensitivity of VZV IgG-specific assays is well-recognised. Thus, the ability of this particular VZV IgG assay to detect both maternal and infant VZV IgG, post-natally, suggests that the earlier VZV IgG negative results were due to lower circulating levels of maternal antibody...
November 23, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29168885/distribution-of-peripheral-dendritic-cells-in-patients-with-hbv-infection-or-spleen-and-kidney-deficiency
#4
Weixia Chen, Lei Wang, Yaofei Niu, Guang Ji, Lianjun Xing
AIMS: Spleen and kidney deficiency syndrome (SKD), a Traditional Chinese Medicine (TCM) syndrome, is the fundamental mechanisms of TCM. We aim to investigate the distribution of peripheral dendritic cells (DCs) in HBV patients with SKD or non-SKD. METHODS: Peripheral venous blood from patients with HBV infection and healthy volunteers was collected to extract PBMC, and flow cytometry assay was used to measure the distribution of DCs subsets, including myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs)...
November 23, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29149532/exploitation-of-micrornas-by-japanese-encephalitis-virus-in-human-microglial-cells
#5
Meghana Rastogi, Neha Srivastava, Sunit K Singh
JEV infection in CNS leads to the JE neuroinflammation. Children and old age individual have been reported to be more prone to JEV infection. MicroRNAs are endogenous, small non-coding RNAs, which regulate the gene expression. These are ∼22 nucleotide long, conserved RNA sequence that binds at the 3'UTR of a target mRNA and regulate the post-transcriptional gene expression. The role of microRNAs has been reported in several diseases like cancer, viral infection, neuro-degeneration, diabetes etc. In the present study, the human microglial cells were infected with JEV (JaOAr strain)...
November 17, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29144554/a-sensitive-colorimetric-dna-biosensor-for-specific-detection-of-the-hbv-gene-based-on-silver-coated-glass-slide-and-g-quadruplex-hemin-dnazyme
#6
Yubin Li, Sheng Liu, Qiujuan Deng, Liansheng Ling
A sensitive colorimetric DNA biosensor for specific detection of single stranded oligonucleotide (ssDNA) is proposed in this paper. The biosensor is based on silver-coated glass (SCGS) and G-quadruplex-hemin DNAzyme. Capture DNA is immobilized on the surface of SCGS by Ag-S bond. Signal DNA can be used to hybridize with the target DNA which is selected from the Hepatitis B virus(HBV) gene as target HBV DNA, and the HRP-mimicking G-quadruplex-hemin DNAzyme can be formed through the function of a guanine-rich fragment from signal DNA to catalyze the oxidation of 2,2-azinobis(3-ethylbenzothiozoline)-6-sulfonicacid (ABTS(2-) ) by H2 O2 ...
November 16, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29144546/presence-of-infection-and-analysis-of-hpv-subtypes-in-girls-younger-than-9-years-old-attended-at-a-referral-service-in-esp%C3%A3-rito-santo-brazil
#7
Mariana Penha De Nadai Sartori, Lidiane Pignaton Agostini, Thaís Tristão Tovar, Raquel Silva Dos Reis, Raquel Spinassé Dettogni, Eldamária de Vargas Wolfgramm Dos Santos, Antônio Chambô Filho, Iúri Drumond Louro
PURPOSE: Human papillomavirus is found in adults and adolescents and is associated with genital warts and cervical cancer. However, it has been detected in girls younger than 10 years old. Currently, there are no prevention methods for this age group, since it is not considered a risk group. The aim of this study was to evaluate the presence of infection and HPV subtype in girls under 9 years old attended at a referral service in the State of Espírito Santo, Brazil. METHODS: Forty-three girls younger than 9 years old had gynecological brush samples collected from vulval and perineal/anal regions...
November 16, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29111616/daclatasvir-and-asunaprevir-treatment-in-patients-infected-by-genotype-1b-of-hepatitis-c-virus-with-no-or-subtle-resistant-associated-substitutions-ras-in-ns5a-y93
#8
Masatoshi Ishigami, Kazuhiko Hayashi, Takashi Honda, Teiji Kuzuya, Yoji Ishizu, Tetsuya Ishikawa, Isao Nakano, Fumihiro Urano, Takashi Kumada, Kentaro Yoshioka, Yoshiki Hirooka, Hidemi Goto
(Backgrounds and Aims) In this study, we investigated the real-world data of the first approved interferon free regimen in Japan; daclatasvir and asunaprevir (DCV+ASV) in chronic hepatitis C patients infected HCV genotype 1b with no or subtle amount of baseline resistant associated substitutions (RAS). (Patients and Methods) Among 924 patients registered in our multicenter study, 750 patients that were proven not to be infected with NS5A-Y93H resistant associated substitutions (RAS) by direct sequencing and to have no or subtle amount (less than 20%) of NS5A-Y93H RAS by probe assays (Cycleave or PCR invader assay) were included in this study...
November 7, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29106738/an-experimental-miniature-piglet-model-for-the-infection-of-human-norovirus-gii
#9
Dong Joo Seo, Day Jung, Soontag Jung, Seung-Kwon Ha, Sang-Do Ha, In-Soo Choi, Jinjong Myoung, Changsun Choi
Ten Yucatan miniature piglets were challenged with the human norovirus (NoV) GII.12/GII.3 CAU140599 strain and five piglets were used as negative controls. Stool, serum, and organs were collected and processed from two NoV-infected piglets and one negative piglet at 1, 2, 3, 5, and 7 days post-inoculation (dpi). NoV was detected in stool and serum samples by real-time RT-PCR. Mild diarrhea was observed at 1-3 dpi. Fecal shedding and viremia were detected intermittently at 1, 3, and 7 dpi. While interferon-α was significantly elevated at 2-3 dpi, interferon-γ was not changed...
November 6, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29091325/dynamics-of-viral-load-in-crimean-congo-hemorrhagic-fever
#10
Imran Hasanoglu, Rahmet Guner, Ahmet Carhan, Zeliha Kocak Tufan, Dilek Yagci Caglayik, Gul Ruhsar Yilmaz, Mehmet Akin Tasyaran
Crimean Congo hemorrhagic fever (CCHF) is a viral zoonotic disease with high mortality rate. There are only a few studies on viral load in CCHF. In our study, we revealed the dynamics of viral load and its relationship with mortality in early phase of the disease. A total of 138 serum samples were collected from 23 patients. All patients had positive PCR for CCHF on admission. Serum samples were obtained daily from all patients for the first 6 days of hospitalization and stored at -80°C for viral load measurement...
November 1, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29091300/serologic-and-viral-genome-prevalence-of-hsv-ebv-and-hcmv-among-healthy-adults-in-wuhan-china
#11
Le Wen, Yong Qiu, Shuang Cheng, Xuan Jiang, Yan-Ping Ma, Wei Fang, Wei Wang, Jie Cui, Qiang Ruan, Fei Zhao, Fei Hu, Min-Hua Luo
The worldwide infection rate of herpesvirus is high, but the detailed prevalence in China, especially the central area, remains unclear. In the present study, the prevalence of Herpes simplex virus (HSV), Epstein-Barr virus (EBV), and human cytomegalovirus (HCMV) was investigated in 303 healthy adults in Wuhan, a representative city in Central China. Viral specific IgG and IgM titers were examined in the serum by chemiluminescent immunoassay, and the existence of viral genomic DNA in blood cells were determined by nested PCR...
November 1, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29091279/hbv-hcv-and-hbv-hcv-co-infection-among-hiv-positive-patients-in-hunan-province-china-regimen-selection-hepatotoxicity-and-antiretroviral-therapy-outcome
#12
Shu Su, Christopher Kincaid Fairley, Joe Sasadeusz, Jianmei He, Xiuqing Wei, Huan Zeng, Jun Jing, Limin Mao, Xi Chen, Lei Zhang
Background Co-infection with hepatitis B (HBV) and C (HCV) is common among people living with HIV (PLHIV). This study investigates the impacts of hepatitis co-infection on antiretroviral therapy (ART) outcomes and hepatotoxicity in PLHIV. Methods The cohort study included 1,984 PLHIV. Hepatotoxicity was defined by elevated alanine aminotransferase (ALT) levels. ART outcomes were measured by CD4 cell counts, viral load and mortality rate in patients. Results Among 1,984 PLHIV, 184 (9.3%) were co-infected with HBV and 198 (10...
November 1, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29083040/metagenomic-analysis-of-viral-diversity-in-respiratory-samples-from-patients-with-respiratory-tract-infections-in-kuwait
#13
N Madi, W Al-Nakib, A S Mustafa, N Habibi
A metagenomic approach based on target independent next-generation sequencing has become a known method for the detection of both known and novel viruses in clinical samples. This study aimed to use the metagenomic sequencing approach to characterise the viral diversity in respiratory samples from patients with respiratory tract infections. We have investigated 86 respiratory samples received from various hospitals in Kuwait between 2015 and 2016 for the diagnosis of respiratory tract infections. A metagenomic approach using the next-generation sequencer to characterise viruses was used...
October 30, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29077213/emergence-of-g9p-8-rotaviruses-in-children-with-acute-gastroenteritis-in-thailand-2015-2016
#14
Wisoot Chan-It, Chulapong Chanta
Human group A rotavirus is a major contagious virus causing gastroenteritis in children. Molecular epidemiological study of group A rotavirus infections in hospitalized children was performed by multiplex RT-PCR during 2015-2016 in Chiang Rai, Thailand. G- and P-genotypes of positive rotavirus samples were further analyzed by one-step and two-step multiplex RT-PCR methods. Among 270 fecal specimens tested, rotavirus was the most prevalent (33.7%), followed by norovirus GII (4.1%), adenovirus (3%), and astrovirus (1...
October 27, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29077212/genetic-variability-human-respiratory-syncytial-virus-subgroups-a-and-b-in-turkey-during-six-successive-epidemic-seasons-2009-2015
#15
Fatma Bayrakdar, Can Naci Kocabaş, Ayşe Başak Altaş, H Gökhan Kavuncuoğlu, Yasemin Coşgun, Emine Dibek Mısırlıoğlu Shahbazi, İhsan Durmaz, Gülay Korukluoğlu, Aykut Özkul
Human respiratory syncytial virus (HRSV) is most important viral respiratory pathogen of acute lower respiratory tract infections in infants and young children worldwide. The circulating pattern and genetic characteristics in the HRSV attachment glycoprotein gene was investigated in Turkey during 6 consecutive seasons from 2009 to 2015. HRSVA was dominant in the all epidemic seasons except 2011-2012 season. Partial sequences of the HVR2 region of the G gene of 479 HRSVA and 135 HRSVB were obtained. Most Turkish strains belonged to NA1, ON1 and BA9, which were the predominant genotypes circulating worldwide...
October 27, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29077211/switching-from-tenofovir-and-nucleoside-analogue-therapy-to-tenofovir-monotherapy-in-virologically-suppressed-chronic-hepatitis-b-patients-with-antiviral-resistance
#16
Dong Yun Kim, Hye Won Lee, Jeong Eun Song, Beom Kyung Kim, Seung Up Kim, Do Young Kim, Sang Hoon Ahn, Kwang-Hyub Han, Jun Yong Park
It is unclear whether chronic hepatitis B (CHB) patients with antiviral resistance, who achieve a complete virologic response (CVR) with tenofovir disoproxil fumarate (TDF) and nucleoside analogue (NUC) combination therapy, maintain CVR if switched to TDF monotherapy. We investigated the persistence of CVR after cessation of NUC in virologically suppressed antiviral resistant CHB patients using TDF+NUC combination therapy. This study recruited 76 antiviral-resistant CHB patients showing CVR on TDF+entecavir (ETV) (n = 52), TDF+lamivudine (LAM; n = 14), and TDF+telbivudine (LdT; n = 10) combination therapy, who were switched to TDF monotherapy as step-down therapy...
October 27, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29077204/hepatitis-b-virus-infection-and-associated-risk-factors-among-pregnant-women-attending-antenatal-care-in-health-facilities-of-tigray-northern-ethiopia
#17
Tadele Araya Mezgebo, Selam Niguse, Atsebaha Gebrekidan Kahsay, Haftamu Hailekiros, Nega Berhe, Tsehaye Asmelash Dejene
Hepatitis B virus infection is one of the leading causes of liver disease in the world. This study was conducted to determine the prevalence of HBV infection and associated risk factors among pregnant women in Northern Ethiopia using a cross-sectional study design. A total of 328 pregnant women were included in this study. Clinical and socio-demographic data of the pregnant women were collected using a structured questionnaire by nurses or midwives during their ANC visit. For the detection of HBsAg, 5 mL of venous blood was collected from the pregnant women; serum was separated in the health facilities from the whole blood and was transported to Tigray Public Health Research Institute for analysis using...
October 27, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29073731/performance-evaluation-of-cepheid-xpert-norovirus-kit-with-a-user-modified-protocol
#18
Rachel Shi-Lei Wong, Fion Yeo, Wai Theng Chia, Chun Kiat Lee, Mun Han Leong, Christopher Wai-Siong Ng, Kok Siong Poon, Gabriel Zherong Yan, Lily-Lily Chiu, Benedict Junrong Yan, Roland Jureen, Evelyn Siew-Chuan Koay, Hong Kai Lee
The Cepheid Xpert® Norovirus kit automates sample processing, nucleic acid extraction, and real-time reverse transcription polymerase chain reactions (RT-PCRs) to detect norovirus genogroups I (GI) and II (GII). Eighty-five stool samples collected between February 2015 and May 2017 were used to compare the performance of a user-modified Xpert assay against a clinically validated laboratory-developed test (LDT). Of the 85 samples, 54 were previously archived in -80°C freezer. The remaining 31 were fresh samples tested concurrently with the LDT...
October 26, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29073730/molecular-and-epidemiological-analysis-of-pandemic-and-post-pandemic-influenza-a-h1n1-pdm09-virus-from-central-india
#19
Mahima Sahu, Neeru Singh, Mohan K Shukla, Varhsa A Potdar, Ravendra K Sharma, Lalit Kumar Sahare, Mahendra J Ukey, Pradip V Barde
Influenza A(H1N1)pdm09 virus pandemic struck India in 2009 and continues to cause outbreaks in its post pandemic phase. Diminutive information is available about influenza A(H1N1)pdm09 from central India. This observational study presents epidemiological and molecular findings for the period of six years. Throat swab samples referred from districts of Madhya Pradesh were subjected to diagnosis of Influenza A(H1N1)pdm09 following WHO guidelines. Clinical and epidemiological data was recorded and analysed. Hemagglutinin (HA) gene sequencing and phylogenetic analysis was performed...
October 26, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29064582/establishment-of-a-platform-for-molecular-and-immunological-characterization-of-the-rna-dependent-rna-polymerase-ns5b-of-an-egyptian-hcv-isolate
#20
Hossam Gewaid, Ahmed A Mesalam, Ahmed A Ibrahim, Dina N Abdel Shafy, Rola N Abdel Shafy, Nahed Emara, Soha M Hamdy, Mohy Gewaid, Mahmoud M Bahgat
The present work aimed at establishing a platform to enable frequent characterization of the HCV RNA-dependent-RNA-polymerase from Egyptian clinical isolates. Subjecting amplified HCV-NS5B coding gene from Egyptian patient's serum to sequencing, multiple alignment, and phylogenetic analysis confirmed its subtype 4a origin. Nucleotide sequence analysis revealed presence of an additional start codon at the beginning of the NS5B gene. Peptide sequence alignment demonstrated presence of unique amino acid residues in our 4a-NS5B sequence distinct from the JFH-1-NS5B sequence as well as unique amino acids compared to other genotypes...
October 24, 2017: Journal of Medical Virology
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