Read by QxMD icon Read

FEMS Microbiology Letters

Angharad E Green, Alejandro Amézquita, Yvan Le-Marc, Matthew J Bull, Thomas R Connor, Eshwar Mahenthiralingam
Pseudomonas aeruginosa (P. aeruginosa) is a common contaminant associated with product recalls in the home and personal care (HPC) industry. Preservation systems are used to prevent spoilage and protect consumers, but greater knowledge is needed of preservative resistance mechanisms used by P. aeruginosa contaminants. We aimed to identify genetic pathways associated with preservative exposure by using an industrial P. aeruginosa strain and implementing RNA-Seq to understand gene expression changes in response to industry relevant conditions...
March 14, 2018: FEMS Microbiology Letters
Tobias Jung, Matthias Mack
We studied the interaction of the tricarboxylic acid cycle (TCA) enzymes citrate synthase, isocitrate dehydrogenase and malate dehydrogenase in the bacteria Bacillus subtilis and Escherichia coli in vivo. In B. subtilis the genes encoding citrate synthase, isocitrate dehydrogenase and malate dehydrogenase form an operon (citZ-icd-mdh) and predominantly are co-transcribed from a single promoter. In E. coli the corresponding genes gltA, icd and mdh do not form a transcription unit, are scattered around the chromosome and are expressed from different promoters...
March 13, 2018: FEMS Microbiology Letters
Jianming Luo, Yuetong Li, Jinli Xie, Lijuan Gao, Liu Liu, Shiyi Ou, Long Chen, Xichun Peng
Bacterial interactions in the biological network affect the growth of Bifidobacterium. In the present study, five habitats were constructed by changing animals, their health statuses and their diets. In each of these habitats, different networks of Bifidobacterium were outlined through correlation analysis of the 50 most dominant microbes. Thirty-eight bacterial genera directly correlated with the growth of Bifidobacterium, including 23 genera with a positive correlation and 15 genera with a negative correlation...
March 13, 2018: FEMS Microbiology Letters
Jia-Yi Cai, Yuan-Yuan Wang, Kai Ma, Yong-Na Hou, Guo-Dong Yao, Toshihiko Hayashi, Kikuji Itoh, Shin-Ichi Tashiro, Satoshi Onodera, Takashi Ikejima
Present study demonstrates that growth of Staphylococcus aureus in the presence of salicylate reduces ultraviolet C (UVC)-induced cell death and increases the generation of reactive oxygen species (ROS). In addition, compounds that scavenge ROS (N-acetylcysteine, glutathione, catalase and superoxide dismutase) reverse the increased UVC survival induced by growth in the presence of salicylate, while ROS donors (tert-butylhydroperoxide, H2O2, and NaClO) enhance survival of salicylate challenged cultures. Collectively these findings suggest that ROS production induced by growth in the presence of salicylate protects S...
March 13, 2018: FEMS Microbiology Letters
Rajesh Padumane Shastry, Stephen K Dolan, Yassmin Abdelhamid, Ravishankar Rai Vittal, Martin Welch
The quorum quenching (QQ) activity of endophytic bacteria associated with medicinal plants was explored. Extracts of the Gram-negative Enterobacter sp. CS66 possessed potent N-acylhomoserine lactone (AHL) hydrolytic activity in vitro. Using degenerate primers, we PCR-amplified an open reading frame (denoted aiiE) from CS66 that was 96% identical to the well-characterised AHL-lactonase AiiA from Bacillus thuringiensis, but only 30% identical to AHL-lactonases from other Gram-negative species. This confirms that close AiiA homologs can be found in both Gram-positive and Gram-negative bacteria...
March 6, 2018: FEMS Microbiology Letters
José Luis Ortega
The main objective of this article is to group altmetric indicators according to their relationships and detect disciplinary differences in a set of 3,793 research articles published in 2013 with regard to their altmetric impact. Three of the most representative altmetric providers (Altmetric, PlumX and CrossRef Event Data (CED)) and Scopus were used to extract information about these publications and their metrics. Principal Component Analysis (PCA) was used to summarize the information of these metrics and detect groups of indicators...
March 6, 2018: FEMS Microbiology Letters
Victoria Hritonenko, Matteo Metruccio, David Evans, Suzanne Fleiszig
The type three secretion system (T3SS) is important for the intracellular survival of Pseudomonas aeruginosa. Known T3SS inducers include low Ca2+, serum, or host cell contact. Here, we used corneal epithelial cell lysates to test if host cytosolic factors could also induce the T3SS. Invasive P. aeruginosa strain PAO1 was exposed to cell lysates for 16 h, and expression of T3SS effectors determined by q-PCR and Western immunoblot. Lysate exposure reduced PAO1 growth (∼5-fold) versus trypticase soy broth (TSB), but also resulted in appearance of a protein in culture supernatants, but not bacteria cell pellets, that reacted with antibody raised against ExoS...
March 6, 2018: FEMS Microbiology Letters
Linfeng Ke, Yajun Chen, Pengming Liu, Shan Liu, Dandan Wu, Yihui Yuan, Yan Wu, Meiying Gao
Magnetotactic bacteria (MTB) can biosynthesize magnetosomes, which have great potential for applications. A new MTB strain, Magnetospirillum sp. ME-1, was isolated and cultivated from freshwater sediments of East Lake (Wuhan, China) using the limiting dilution method. ME-1 had a chain of 17 ± 4 magnetosomes in the form of cubooctahedral crystals with a shape factor of 0.89. ME-1 was closest to Magnetospirillum sp. XM-1 according to 16S rRNA gene sequence similarity. Compared with XM-1, ME-1 possessed additional copy of mamPA and a larger mamO in magnetosome-specific genes...
March 4, 2018: FEMS Microbiology Letters
Sofia Mindlin, Anatoliy Petrenko, Mayya Petrova
A novel mobile genetic element has been identified in small plasmids isolated from permafrost strains of Acinetobacter lwoffii. This element, designated the chrAB dif module, contains the chromium resistance genes chrA and chrB, functionally active both in the original host strains and after transfer into Acinetobacter baylyi. The 3011 bp chrAB dif module is flanked by XerC/XerD recombination sites highly homologous to those of the site-specific recombination system dif/Xer. Analysis of public databases revealed almost identical variants of the chrAB dif module in different plasmids in strains of various Acinetobacter species predominantly inhabiting the environment (A...
March 4, 2018: FEMS Microbiology Letters
Rongmei Li, Shuo Wang, Ronghua Sun, Xiang He, Yongting Liu, Congfeng Song
Transcription activator-like (TAL) effectors encoded by tal genes were recognized as a key virulence strategy used by Xanthomonas oryzae pv. oryzae (Xoo) to cause bacterial leaf blight of rice. TAL effector PthXo3 is a major virulence factor identified in a Philippine Xoo strain PXO61, and it can induce the expression of susceptibility gene OsSWEET14 by binding to the effector-binding element (EBE) in the promoter region. In this study, pthXo3 homologous genes were also identified and isolated from Xoo Chinese strain OS198 and Japanese strain JXOV, which were named as PthXo3OS198 and PthXo3JXOV respectively...
March 4, 2018: FEMS Microbiology Letters
Shunzhang Liu, Canxin Meng, Guanpeng Xu, Huahua Jian, Fengping Wang
Reference genes are critical to obtain reliable results of RT-qPCR, which is widely used for relative quantification of gene expression. In this study, we evaluated the validity of seven candidate reference genes for normalization in RT-qPCR analysis in the deep-sea bacterium Shewanella psychrophila WP2 under different environmental conditions. Among the set of genes investigated, gyrA, 16S rRNA and rho were identified as the most suitable reference genes for WP2 at different temperatures, hydrostatic pressures and salinities, respectively...
February 26, 2018: FEMS Microbiology Letters
Chenlang Liu, Fangnan Yu, Qingshu Liu, Xiaoying Bian, Shengbiao Hu, Huansheng Yang, Yulong Yin, Yuezhong Li, Yuemao Shen, Liqiu Xia, Qiang Tu, Youming Zhang
Transporter engineering has been shown to be a positive approach for enhancing natural product titers in microbial cell factories by expelling target compounds out of feasible hosts. In this work, two multidrug efflux pumps, Orf14 and Orf3, were modulated in the epothilone production strain Burkholderia DSM7029::Tn5-km-epo (named G32) via Red/ET engineering to increase heterologous polyketide epothilone yields. Compared with the prior G32 strain, the total production of several epothilones in the G32::orf14-orf3 mutant was meaningfully doubled according to high-performance liquid chromatography-mass spectrometer (HPLC-MS) analysis...
February 26, 2018: FEMS Microbiology Letters
Syed Hidayathulla, Abdelaaty A Shahat, Mansour S Alsaid, Abdullah A Al-Mishari
The present study investigates the optimization of tannase production from A. nidulans for various physicochemical parameters and harvests tannase for its chemical characterization. The maximum tannase activity was observed on the third day of incubation at 35°C and the stability was observed at pH 5.5 to 6.0 by holding its 100% activity. The tannase was partially purified from A. nidulans [FT10] by ammonium sulfate precipitation at different concentrations and it was found that at 80% of ammonium sulfate concentration, the precipitate exhibited the maximum activity for tannase of 96 U/ml...
February 26, 2018: FEMS Microbiology Letters
Jorge Mejía-Barajas, Rocío Montoya-Pérez, Salvador Manzo-Avalos, Christian Cortés-Rojo, Héctor Riveros-Rosas, Carlos Cervantes, Alfredo Saavedra-Molina
Membrane fatty acid composition has an important role in yeast stress resistance, particularly in temperature tolerance. Most studies investigating temperature and membrane fatty acids use the yeast Saccharomyces cerevisiae without considering other yeasts, such as Kluyveromyces marxianus, which has physiological differences and industrial advantages with respect to Saccharomyces cerevisiae. One of the primary traits of Kluyveromyces marxianus is its thermotolerance. The effect of fatty acid addition (oleic acid, linoleic acid, linolenic acid, and araquidic acid) on the thermotolerance of the Kluyveromyces marxianus strain SLP1 was evaluated...
February 22, 2018: FEMS Microbiology Letters
Chuanqing Zhong, Jiafang Fu, Tianyi Jiang, Chunming Zhang, Guangxiang Cao
The ability of Microlunatus phosphovorus to accumulate large amounts of polyphosphate (Poly-P) plays an important role in removing soluble phosphorus from wastewater. Strain JN459, isolated from a sewage system, was previously demonstrated to be Microlunatus phosphovorus. In this study, we analyzed the phosphorus-accumulating and phosphorus-releasing characteristics of strain JN459. Our analyses indicate that strain JN459 accumulates Poly-P under aerobic conditions but releases phosphorus under anaerobic conditions...
February 22, 2018: FEMS Microbiology Letters
Kristina Beblo-Vranesevic, Maria Bohmeier, Alexandra K Perras, Petra Schwendner, Elke Rabbow, Christine Moissl-Eichinger, Charles S Cockell, Pauline Vannier, Viggo T Marteinsson, Euan P Monaghan, Pascale Ehrenfreund, Laura Garcia-Descalzo, Felipe Gómez, Moustafa Malki, Ricardo Amils, Frédéric Gaboyer, Frances Westall, Patricia Cabezas, Nicolas Walter, Petra Rettberg
Four facultative anaerobic and two obligate anaerobic bacteria were isolated from extreme environments (deep subsurface halite mine, sulfidic anoxic spring, mineral-rich river) in the frame MASE (Mars Analogues for Space Exploration) project. The isolates were investigated under anoxic conditions for their survivability after desiccation up to six months and their tolerance to ionizing radiation up to 3000 Gy. The results indicated that tolerances to both stresses are strain-specific features. Yersinia intermedia MASE-LG-1 showed a high desiccation tolerance but its radiation tolerance was very low...
February 21, 2018: FEMS Microbiology Letters
Bingjun Qian, Lirong Yin, Xiaomin Yao, Yaoguang Zhong, Juan Gui, Feifeng Lu, Fuming Zhang, Jianhua Zhang
The saponins, as components of tea seed meal (TSM), are undesirable hemolytic components, and should be degraded for reducing their hemolytic activity in order to be used in animal feed. In this study, β-glucuronidase was verified to be a potent hydrolase of tea seed saponins to reduce their hemolytic activity, and a β-glucuronidase-producing Lactobacillus crustorum (L. crustorum) strain was screened from raw bovine milk. Next, solid-state fermentation with the isolated L. crustorum and a Bacillus subtilis natto strain, which can produce cellulase and hence improve the fermentation performance of tea seed meal, was processed for detoxification of tea seed meal...
February 20, 2018: FEMS Microbiology Letters
Miriam B Herold, Madeline E Giles, Colin J Alexander, Elizabeth M Baggs, Tim J Daniell
Denitrification is a key process responsible for the majority of soil nitrous oxide (N2O) emissions but the influences of pH and cultivation on the soil denitrifier community remain poorly understood. We hypothesised that the abundance and community structure of the total bacterial community and bacterial denitrifiers would be pH sensitive and that nirK and nirS containing denitrifiers would differ in their responses to change in pH and cultivation. We investigated the effect of long-term pH adjusted soils (ranging from pH 4...
February 19, 2018: FEMS Microbiology Letters
Ashutosh Tripathi, Vishal Srivastava, Bhupendra N Singh
Tuberculosis continues to be one of the deadliest infectious disease worldwide. MicroRNAs (miRNAs) are small non-coding entities that play critical role as post-transcriptional regulators and are transcriptionally deregulated upon mycobacterial infection. In this study, we found significant upregulation of hsa-let-7b-5p in Mycobacterium tuberculosis (MTB) infected THP-1 human macrophages. Concomitantly, we detected the reduced level of Fas protein, one of the targets of hsa-let-7b-5p, in MTB infected THP-1 macrophages...
February 19, 2018: FEMS Microbiology Letters
María J Torres, Sergio Avila, Eulogio J Bedmar, María J Delgado
The alfalfa endosymbiont Ensifer meliloti strain1021 is known to be an incomplete denitrifier due to its inability to grow anoxically using nitrate as respiratory substrate to produce ATP and grow under anoxic conditions. Although this bacterium contains and expresses the complete set of denitrification genes napEFDABC, nirK, norECBQD and nosRZDFYLX encoding the periplasmic nitrate reductase (Nap), Cu-containing nitrite reductase (NirK), c-type nitric oxide (cNor) and nitrous oxide reductase (Nos), respectively, the reasons of its inability to grow under anoxic conditions are still very poorly understood...
February 16, 2018: FEMS Microbiology Letters
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"