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FEMS Microbiology Letters

Ignatiy I Goryanin, Anna A Kudryavtseva, Vladimir P Balabanov, Valentina S Biryukova, Ilya V Manukhov, Gennadii B Zavilgelsky
Antirestriction proteins of the ArdB group (ArdB, KlcA) specifically inhibit restriction (endonuclease) activity of restriction-modification (RM) type I systems. Anti-restriction activity of KlcA and ArdB, encoded in transmissible plasmids RP4 (IncPα) and R64 (IncI1), respectively, has been determined. We show that the protein KlcA (RP4), an amino acid sequence identical to that of the protein KlcA (RK2), inhibits the activity of EcoKI when the klcA gene is located on the plasmid under the control of strong promoter...
September 18, 2018: FEMS Microbiology Letters
F G Melgaço, I S Luz, M R S Assis, M S Caldas, A G Maranhão, D A F Silva, M L L Brandão, V M Medeiros, C O Rosas, S M L Reis, M P Miagostovich
This study aimed to evaluate viral and bacterial contamination from typical Brazilian cheeses, such as Minas (fresh) and Prato (ripened), commercially obtained in the Greater Metropolitan Region of the State of Rio de Janeiro, Brazil. Minas [30], Prato [30] and sliced Prato [30] cheese samples were investigated for norovirus genogroup I and II (NoV GI-II) and human adenovirus (HAdV) by direct nucleic acid extraction using TRIzol® and amplification by TaqMan® based quantitative polymerase chain reaction. Listeria monocytogenes, Salmonella spp...
September 18, 2018: FEMS Microbiology Letters
Katharina Novak, Stefan Pflügl
A broad range of different chemical and pharmaceutical compounds have been produced in microbial cell factories. To compete with traditional crude oil based production processes, the use of complex alternative raw materials such as lignocellulosic biomass, waste streams and utilization of CO2 in gas fermentations has been suggested. All of these streams contain acetate, a cheap and potentially interesting carbon source for microbial production processes. Acetate (co-)utilization remains challenging, which is the reason for extensive research on the use of acetate for the production of value-added compounds...
September 18, 2018: FEMS Microbiology Letters
Lincon Felipe Lima Silva, Aline Sotta Réco, Rúben Peña, Maria Angelica Ganga, Sandra Regina Ceccato-Antonini
Dekkera bruxellensis is a spoilage yeast in wine and fuel ethanol fermentations able to produce volatile phenols from hydroxycinnamic acids by the action of the enzymes cinnamate decarboxylase (CD) and vinyphenol reductase (VR) in wine. However, there is no information about this ability in the bioethanol industry. This work evaluated CD and VR activities and 4-ethylphenol production from p-coumaric acid by three strains of D. bruxellensis and PE-2, an industrial Saccharomyces cerevisiae strain. Single and multiple-cycle batch fermentations in molasses and sugarcane juice were carried out...
September 18, 2018: FEMS Microbiology Letters
Jack T H Wang, Wilhelmina M Huston, Priscilla Johanesen, Megan Lloyd, Karena L Waller
The American Society for Microbiology's curricular guidelines for Introductory Microbiology highlighted key laboratory skills in the isolation, visualization, and identification of microorganisms as core learning objectives in the discipline. Since the publication of these guidelines in 2012, there has been a paucity of diagnostic assessment tools in the literature that can be used to assess competencies in the microbiology laboratory. This project aimed to establish a laboratory competency examination for introductory microbiology, with tasks specifically aligned to laboratory skills and learning outcomes outlined in curricular guidelines for microbiology...
September 18, 2018: FEMS Microbiology Letters
Sara Amiri Fahliyani, Keivan Beheshti-Maal, Fereshteh Ghandehari
Mastitis is an inflammation of the mammary gland that occurs when pathogenic microorganisms enter the udder. Even though tremendous advancements in veterinary diagnosis and therapeutics, mastitis is still the most frequent and costly disease of dairy herds overall the world. The purpose of this research was to isolate and identify the lytic phages as a potential method for biological control of bovine mastitis. In this study Klebsiella oxytoca was isolated from contaminated milk samples of Isfahan dairy herds, Isfahan, Iran and characterized as K...
September 13, 2018: FEMS Microbiology Letters
Sharrel Rebello, Amith Abraham, Aravind Madhavan, Raveendran Sindhu, Parameswaran Binod, Arun K Babu, Embalil Mathachan Aneesh, Ashok Pandey
The non-conventional yeasts Kluyveromyces lactis, Yarrowia lipolytica, Ogataea polymorpha and Pichia pastoris have been developed as eukaryotic expression hosts because of their desirable growth characteristics, including inhibitor and thermo-tolerance, utilization of diverse carbon substrates, and high amount of extracellular protein secretion. These yeasts already have established in the heterologous production of vaccines, therapeutic proteins, food additives, and bio-renewable chemicals, but recent advances in genetic tool box have the potential to greatly expand and diversify their impact on biotechnology...
September 13, 2018: FEMS Microbiology Letters
Yuko Ohara-Nemoto, Yu Shimoyama, Manami Nakasato, Haruka Nishimata, Taichi Ishikawa, Minoru Sasaki, Shigenobu Kimura, Takayuki K Nemoto
Dipeptidyl peptidase (DPP) 4, DPP5, DPP7, and DPP11, expressed in the periplasmic space, are crucial for energy production for Porphyromonas gingivalis, an asaccharolytic bacterium that causes periodontal disease. Bacterial DPP4 seems to be involved in regulation of blood glucose level via degradation of incretins. The present study aimed to identify four dpp orthologs in oral microbiota by database searches, and their enzymatic activities in periodontopathic and cariogenic bacteria, as well as oral specimens were determined...
September 10, 2018: FEMS Microbiology Letters
Fu Lina, Wang Ting, Wei Lanfang, Yang Jun, Liu Qi, Wang Yating, Wang Xing, Ji Guanghai
Lysobacter antibioticus is an important biocontrol bacteria against phytopathogens in soil and with the ability to produce nonvolatile antimicrobial metabolites has been extensively characterised. It's important to estabilish application techniques to detect and monitor L. antibioticus directly and acurately in soil samples. We developed and tested 13 primer sets according to phenazine gene (phzA, phzB, phzD, phzF, phzS) and cyclohexanone monooxygenase gene (phzNO1), a pair of primer phzNO1 F1/phzNO1 R1 based on cyclohexanone monooxygenase (phzNO1) gene of L...
September 10, 2018: FEMS Microbiology Letters
Tomotaka Tanabe, Ayaka Isshiki, Katsushiro Miyamoto, Hiroshi Tsujibo, Shigeo Yamamoto, Tatsuya Funahashi
We found that Vibrio furnissii can utilize aerobactin (AERO) as a xenosiderophore. A homology search of its genome revealed that this bacterium possesses genes encoding an AERO-mediated iron acquisition system similar to that of Vibrio vulnificus. The system consists of the ABC transporter gene vatCDB, the GntR-type transcriptional repressor gene iutR, and the outer membrane receptor gene iutA. The functions of the vatCDB operon and iutA in V. furnissii were confirmed by the inability of the corresponding deletion mutants to utilize AERO...
September 7, 2018: FEMS Microbiology Letters
Delia Patricia Parrilla-Taylor, Norberto Vibanco-Pérez, Ma de Jesús Durán-Avelar, Bruno Gomez-Gil, Raúl Llera-Herrera, Ricardo Vázquez-Juárez
White spot syndrome virus (WSSV) has a ∼300 kb double-stranded DNA genome. It originated in China, spread rapidly through shrimp farms in Asia, and subsequently to America. This study determined complete genome sequences for nine historic WSSV strains isolated from Pacific white shrimp (Litopenaeus vannamei) captured in farm ponds in northwest Mexico (Sinaloa and Nayarit). Genomic DNA was captured by an amplification method using overlapping long-range PCR and sequencing by Ion Torrent-PGM. Complete genome sequences were assembled (length range 255-290 kb) and comparative genome analysis with WSSV strains revealed substantial deletions (3 and 10 kb in two regions) in seven strains, with two strains differing from the rest...
September 4, 2018: FEMS Microbiology Letters
Thuan Phu Nguyen-Vo, Satish Kumar Ainala, Jung-Rae Kim, Sunghoon Park
Coenzyme B12 is an essential cofactor for many enzymes such as glycerol dehydratase, methionine synthase and methylmalonyl-CoA mutase. Herein, we revisited the B12 biosynthetic gene clusters (I and II) in Pseudomonas denitrificans, a well-known industrial producer of the coenzyme B12, to understand the regulation of gene expression and improve the production of coenzyme B12. There existed eight operons, seven in the cluster I and one in the cluster II, and four operons were regulated by B12-responsive riboswitches with a switch-off concentration at ∼5 nM coenzyme B12...
September 3, 2018: FEMS Microbiology Letters
Rui-Ming Wang, Nan Li, Kai Zheng, Jing-Feng Hao
Enhancing the acid tolerance of the probiotic bacterial strain Lactobacillus acidophilus NCFM (L. acidophilus NCFM) is critical for improving its viability during transiting through gastrointestinal tract, which is the basis of eliciting beneficial effects as an intestinal probiotic. In this study, the role of trehalose and the glutamateγ-aminobutyric acid antiporter gene gadC in enhancing the acid tolerance of L. acidophilus NCFM was investigated. Trehalose at a concentration of 0.5% (w/v) was sufficient to significantly increase bacterial viability at pH 2...
September 3, 2018: FEMS Microbiology Letters
Quan Xie, Wen Mei, Xiang Ye, Peng Zhou, Md Sharifull Islam, Khalid Ragaei Abdraboh Elbassiony, Chao Yuan, Jinquan Li, Yang Zhou
Aeromonas hydrophila is a causative pathogen of many infectious diseases in fish and human. The two-component regulatory system enables bacteria to response to a wide range of stimuli, growth conditions and environments. The two-component system CpxA/R is prevalent in microorganisms and involved in the pathogenesis of a number of pathogens. In this study, we generated a ΔcpxA/R mutant of A. hydrophila ZYAH72 to explore the biological functions mediated by the CpxA/R in this organism. Compared to the wild type strain, the knockout mutant strain ΔcpxA/R exhibited a significant impairment in adherence to human epithelial cells Caco-2 and resistance against host killing in fish blood...
September 3, 2018: FEMS Microbiology Letters
Xian-Liang Zhao, He Chen, Ke-Ke Zhong, Li Li, Xiang-Hui Kong
Florfenicol, a synthetic drug with chemical structure and spectrum of antibacterial activity similar to chloramphenicol, has been shown to be effective against a number of bacterial pathogens. However, there are increasing signs of florfenicol-resistant bacteria due to the misuse and overuse of florfenicol in aquaculture. In the present study, florfenicol had a higher bactericidal efficacy in the presence of myo-inositol, may be due to the ability of myo-inositol to increase susceptibility of Aeromonas hydrophila to florfenicol...
September 3, 2018: FEMS Microbiology Letters
Kenji Okano, Kohsuke Honda, Hironori Taniguchi, Akihiko Kondo
Recent advances in DNA synthesis and computer science have enabled the de novo design of biosynthetic pathways. Numerous computational tools are currently available for searching biosynthetic pathways and ranking them on the basis of multiple criteria for installation into microbial chassis strains. This new framework allows the design of artificial biosynthetic pathways without expert knowledge of the specific biochemical reactions involved. Moreover, genetic apparatuses with quantitative and predictable properties enable rational construction of gene circuits...
August 30, 2018: FEMS Microbiology Letters
Rajni Hatti-Kaul, Lu Chen, Tarek Dishisha, Hesham El Enshasy
Lactic acid bacteria constitute a diverse group of industrially significant, safe microorganisms that are primarily used as starter cultures and probiotics, and are also being developed as production systems in industrial biotechnology for biocatalysis and transformation of renewable feedstocks to commodity- and high value chemicals, and health products. Development of strains, which was initially based mainly on natural approaches, is also achieved by metabolic engineering that has been facilitated by the availability of genome sequences and genetic tools for transformation of some of the bacterial strains...
August 30, 2018: FEMS Microbiology Letters
Chih-Chan Wu, Yung-Yu Tsai, Takao Ohashi, Ryo Misaki, Savitree Limtong, Kazuhito Fujiyama
Oleaginous yeast Rhodosporidium toruloides DMKU3-TK16 (TK16), which was isolated from Thailand, is considered a promising lipid producer for biodiesel production. For future industrial applications of this strain, thermotolerant traits are highly desired for their potential to reduce cooling costs in a commercial fermenter. Here, by using an adaptive breeding strategy, we isolated a thermotolerant R. toruloides mutant, L1-1. The isolated L1-1 strain exhibited better growth and higher lipid production at 37°C, and it was found to have significantly higher oleic acid (C18:1) content and yield compared to the wild-type TK16 when cultivated at 37°C...
August 17, 2018: FEMS Microbiology Letters
Sydney Pindling, Daniella Azulai, Brandon Zheng, Dylan Dahan, Gabriel G Perron
Exposure to low concentrations of antibiotics found in aquatic environments can increase susceptibility to infection in adult fish due microbiome disruption. However, little is known regarding the effect of antibiotic pollution on fish larvae. Here, we show that exposure to streptomycin, a common antibiotic used in medicine and aquaculture, disrupts the normal composition of zebrafish larvae microbiomes, significantly reducing the microbial diversity found in the fish. Exposure to streptomycin also significantly increased early mortality among fish larvae, causing full mortality within a few days of exposure at 10 μg/mL...
July 19, 2018: FEMS Microbiology Letters
Akira Fukuda, Masaru Usui, Torahiko Okubo, Chie Tagaki, Nop Sukpanyatham, Yutaka Tamura
The spreading of antimicrobial-resistant Enterobacteriaceae, especially those co-harboring plasmid-mediated cephalosporin (bla) and colistin (mcr) resistance genes, is becoming increasingly problematic. As a vector, flies carry antimicrobial-resistant bacteria (ARB) into human and livestock habitats. To investigate ARB in flies, we collected 235 flies from 27 sites (18 urban areas, five pig farms, and four chicken farms) in Thailand during 2013-2015. Cefotaxime-resistant Enterobacteriaceae (CtxRE) and bla-positive CtxRE were isolated from 70 (29...
July 13, 2018: FEMS Microbiology Letters
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