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FEMS Microbiology Letters

Chettida Srisuksam, Juntira Punya, Songsak Wattanachaisaereekul, Wachiraporn Toopaang, Supapon Cheevadhanarak, Morakot Tanticharoen, Alongkorn Amnuaykanjanasin
pks14, a reducing clade IIb polyketide synthase gene, is preserved throughout the evolution of entomopathogenic fungi. We examined the functions of pks14 in Beauveria bassiana using targeted gene disruption. pks14 disruption was verified by Southern blot and PCR analyses. The radial growth, cell dry weight and conidial germination of Δpks14 were comparable to that of the wild type. Our sequence and gene expression analyses of the pks14 biosynthetic cluster demonstrated: (i) cotranscription and constitutive expression of nearly all genes of the aforementioned cluster including C2H2 zinc finger transcription regulator gene, but not pks14 and cytochrome P450 gene; (ii) expression of pks14 gene in insect-containing culture condition only; whereby (iii) a KAR9-like gene in direct proximity with pks14 is the only gene showing co-regulation...
May 22, 2018: FEMS Microbiology Letters
Jianhua Yin, Jingxiao Cai, Zhe Yuan, Zhewei Wang, Haichun Gao, Juanping Qiu, Zhiliang Yu
High molecular weight penicillin binding proteins (PBPs) are responsible for the biosynthesis of peptidoglycan. In Escherichia coli, PBP1a and PBP1b form multienzyme peptidoglycan-synthesizing complexes with outer-membrane lipoproteins LpoA and LpoB, respectively. The two complexes appear to be largely redundant, although their distinct physiological roles remain unclear. PBP1a/LpoA and PBP1b/LpoB also exist in Shewanella oneidensis strain MR-1, but effects of the two complexes on aerobic growth and β-lactam resistance are quite different...
May 17, 2018: FEMS Microbiology Letters
Estibaliz Mateo, Elena Sevillano
In the recent years, there has been a decrease in the number of medical professionals dedicated to a research career. There is evidence that students with a research experience during their training acquire knowledge and skills that increase the probability of getting involved in research more successfully. In the Degree of Medicine (University of the Basque Country) the annual core subject 'Research Project' introduces students to research. The aim of this work was to implement a project-based learning methodology, with the students working on microbiology, and to analyse its result along time...
May 17, 2018: FEMS Microbiology Letters
Anna Espinoza Tofalos, Matteo Daghio, Myriam González, Maddalena Papacchini, Andrea Franzetti, Michael Seeger
Bioelectrochemical remediation of hydrocarbons is a technology that exploits the ability of specific microorganisms to use as electron acceptor an electrode, thus potentially lowering the operational costs related to classical bioremediation. Several well-characterized hydrocarbonoclastic strains might be electroactive, thus their biodegradation performances in Bioelectrochemical Systems should be studied. Cupriavidus metallidurans CH34 is a model metal-resistant strain whose capacity to degrade benzene aerobically has recently been described...
May 16, 2018: FEMS Microbiology Letters
Haixiang Qiu, Jiansen Gong, Patrick Butaye, Guangwu Lu, Ke Huang, Guoqiang Zhu, Jilei Zhang, Terri Hathcock, Darong Cheng, Chengming Wang
Quinolones are broad-spectrum antibiotics that have been used for decades in treating bacterial infections in humans and animals, and subsequently bacterial resistance to these agents has increased. While studies indicated the relationship between gyrA mutations and bacterial resistance to quinolones, CRISPR/Cas9 was used in this study to investigate causal role of gyrA mutation in the quinolone resistance. In this study, 818 clinical Escherichia coli isolates were analyzed for gyrA mutations and their resistance to quinolones...
May 14, 2018: FEMS Microbiology Letters
Hana Salmonová, Jirí Killer, Vera Bunešová, Martina Geigerová, Eva Vlková
Pectinatella magnifica is a freshwater bryozoan, which has become a subject of scientific interest because of its invasive expansion worldwide. To obtain a comprehensive overview of its influence on environments, information on associated bacteria is needed. In this study, cultivable bacteria associated with P. magnifica were investigated. In total, 253 isolates were selected for preliminary identification by MALDI-TOF mass spectrometry and clustered based on REP-PCR profiles. Among these, 169 strains were selected and identified using 16S rRNA gene comparative analyses...
May 14, 2018: FEMS Microbiology Letters
Lucia Muggia, Sigrun Kraker, Theodora Gößler, Martin Grube
The thallus structure of the lichen symbiosis provides a fungal shelter for the growth of algal partners. The long-living thallus also provides a habitat for other fungi, but experimental studies, which could inform us about the details of their interactions have hardly been conducted. We present a new approach by embedding axenically cultured strains of fungi together with algae in alginate spheres, which allows easy transfer of co-cultures on solid media. As the growth rates of the organisms are differentially triggered by the underlying medium, alginate embedding can help to adjust optimal parameters for stable culture of the combined symbionts...
May 4, 2018: FEMS Microbiology Letters
Rabia Tanvir, Aqeel Javeed, Yasir Rehman
Fatty acid and their amide derivatives are natural self-defense agents in plants. They have been observed to be broadly bioactive against a variety of disease agents. The mechanism of action understood so far being their targeting the protein synthesis and causing leakage of the intracellular components. Owing to their broad bioactivity, the fatty acids and their amides as therapeutics can cover a wide range of indications such as cancer, bacterial infections, parasitic infection, inflammations, diabetes and obesity to name a few...
May 3, 2018: FEMS Microbiology Letters
Weiming Yao, Guangjian Xu, Bing Bai, Hongyan Wang, Minggui Deng, Jinxin Zheng, Duoyun Li, Xiangbin Deng, Xiaojun Liu, Zhiwei Lin, Zhong Chen, Guiqiu Li, Qiwen Deng, Zhijian Yu
The aim of this study was to determine whether in vitro induced erythromycin resistance facilitates the cross-resistance to the novel fluoroketolide, solithromycin, in Staphylococcus aureus. Four strains of methicillin-susceptible S. aureus strains S2, S3, S5, S7 were successfully induced to establish erythromycin-resistant strains by continuous in vitro culture with erythromycin. Mutations at drug binding sites were shown to increase the minimal inhibitory concentrations for ketolides, including telithromycin and the novel compound solithromycin, but did not increase for lincosamides, chloramphenicols, or oxazolidinones...
May 3, 2018: FEMS Microbiology Letters
Jean-Claude Piffaretti, Bernhard Schink, Jan C Semenza
No abstract text is available yet for this article.
April 30, 2018: FEMS Microbiology Letters
L Ganderton
Historically, the vital roles played by technicians in Higher Education have had little recognition. Job opportunities are often overlooked by those who have no working knowledge of such organisations and poorly defined career pathways mean that it may not be a first choice for well-qualified, ambitious individuals. With just over a third of all Higher Education technicians in the UK now due to retire in the next 10 to 15 years, fundamental changes are needed, both to attract large numbers of people into vacant roles and to retain current technical staff...
April 27, 2018: FEMS Microbiology Letters
Tomoya Tsukazaki
The bacterial membrane protein SecDF enhances protein translocation across the membrane driven by the complex of SecA ATPase and SecYEG. Many newly synthesized proteins in the cytoplasm are programmed to be translocated to the periplasm via the narrow channel that is formed in the center of SecYEG. During the protein translocation process, SecDF is proposed to undergo repeated conformational transitions to pull out the precursor protein from the SecYEG channel into the periplasm. Once SecDF captures the precursor protein on the periplasmic surface, SecDF can complete protein translocation even if SecA function is inactivated by ATP depletion, implying that SecDF is a protein translocation motor that works independent of SecA...
April 27, 2018: FEMS Microbiology Letters
L A Robinson, I J Turner, M J Sweet
No abstract text is available yet for this article.
April 26, 2018: FEMS Microbiology Letters
Carlos Felipe Estévez Castro, Jorge Humberto Serment-Guerrero, Jorge Luis Fuentes
Exposure to ultraviolet (UV) radiation blocks DNA replication and arrests cellular division in Escherichia coli. Restoration of chromosome replication involves nucleoid reorganization, which involves the participation of the recombination-catalysing proteins RecA, RecO, RecR and RecN. In the present work, we evaluated the influence of recN, uvrA and recJ gene mutations on post-irradiation nucleoid reorganization. We used isogenic E. coli strains that are defective for these genes to study post-irradiation kinetics of the nucleoid shape fractions using fluorescence microscopy...
April 26, 2018: FEMS Microbiology Letters
Halie K Miller, Whittney N Burda, Ronan K Carroll, Lindsey N Shaw
Staphylococcus aureus possess three alternative σ factors, including a lone extracytoplasmic function σ factor, σS. Our group previously identified and characterized this element, mapping three sigS promoters, demonstrating its inducibility during stress and virulence inducing conditions, and demonstrating a role for this factor in disease causation. In the present study, we identify a fourth promoter of the sigS operon, termed P4, located in a unique position internal to the sigS coding region. Transcriptional profiling revealed that expression from P4 is dominant to the three upstream promoters, particularly upon exposure to chemical stressors that elicit DNA damage and disrupt cell wall stability; each of which have previously been shown to stimulate sigS expression...
April 24, 2018: FEMS Microbiology Letters
Nicola J Fawcett, Anna Dumitriu
The field of microbiology presents unique opportunities, and accompanying challenges, for artistic collaborations. On one hand, artistic works enable exploration of the aesthetics and of issues in biomedical science and new technologies, and draw in new, non-scientific audiences. On the other hand, creating art with microbes requires rigorous consideration of health and safety.Artists working in this field, known as Bio Art, tend to want to push the boundaries of what is possible or 'known', and work with new biomedical tools as they become available...
April 24, 2018: FEMS Microbiology Letters
Daniel Lipus, Dhritikshama Roy, Eakalak Khan, Daniel Ross, Amit Vikram, Djuna Gulliver, Richard Hammack, Kyle Bibby
The Bakken Shale has become one of the United States' most important oil and gas producing regions. This study examined the microbiology and geochemical characteristics of Bakken region produced water from 17 well sites sampled from the three-phase separator and produced water holding tank over a six-month time frame. Produced water samples had high total dissolved solids (TDS) (220,000 mg/L - 350,000 mg/L) and low dissolved organic carbon (DOC) concentrations (41 mg/L - 132 mg/L). Microbial abundances varied between 101-104 16S rRNA gene copies/mL, approximately four orders of magnitude below those observed for produced waters from other hydraulic fracturing regions...
April 23, 2018: FEMS Microbiology Letters
Chen Hanna, Fang Qian, Tu Qiang, Liu Chenlang, Yin Jia, Yin Yulong, Xia Liqiu, Bian Xiaoying, Zhang Youming
Contact-dependent growth inhibition (CDI) is a type of competitive mechanisms, and has been identified in various strains including Burkholderia, Dickeya, E. coli, and Yersinia. Classical CDI systems contain three genes, cdiB, cdiA and cdiI. CdiB encoded by cdiB gene is a conserved β-barrel protein and required for export of CdiA. CdiA protein encoded by cdiA gene includes a conserved N-terminal domain, and variable C-terminal toxic domain (CdiA-CT). Immunity protein CdiI binds and inactivates toxin protein CdiA-CT...
April 23, 2018: FEMS Microbiology Letters
Tammy C Tobin, Ashley Shade
Microbiology increasingly relies upon bioinformatics to understand complex microbial interactions. Nevertheless, biology undergraduates often lack the basic quantitative and computer-based skills required for bioinformatics analyses. To address these issues, the course module 'A Town on Fire! 16S rRNA Gene Amplicon Analysis of Microbial Communities Overlying the Centralia, PA Mine Fire' was developed for an undergraduate microbiology lecture course. In this module, microbiology students used Quantitative Insights into Microbial Ecology (QIIME) to perform taxonomic, phylogenetic and statistical analyses on bacterial communities from three hot mine fire-impacted surface soils using 16S rRNA gene amplicon sequences...
April 23, 2018: FEMS Microbiology Letters
Chitwadee Phithakrotchanakoon, Aekkachai Puseenam, Sriwan Wongwisansri, Lily Eurwilaichitr, Supawadee Ingsriswang, Sutipa Tanapongpipat, Niran Roonsawang
Ogataea thermomethanolica TBRC656 is a thermotolerant methylotrophic yeast suitable for heterologous protein expression at various temperatures. However, the lack of efficient methods for targeted gene mutagenesis limits strain engineering in this yeast. In this study, we applied a CRISPR-Cas9 based-tool for targeted gene mutagenesis in O. thermomethanolica. The putative unfolded protein response (UPR) regulator OtHAC1, and the OtMAL1 (maltase) and OtMAL2 (maltose permease) genes involved with sucrose and maltose utilization were targeted for CRISPR-Cas9 mutagenesis...
April 23, 2018: FEMS Microbiology Letters
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