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FEMS Microbiology Letters

Gaurav Kumar, Bagre Issa, Debasish Kar, Sarmistha Biswal, Anindya S Ghosh
New Delhi Metallo beta-lactamase (NDM) is of significant public health concern due to its enormous potential to hydrolyse all major beta-lactams including carbapenems. Amino acid substitutions outside the active site reportedly affect NDM beta-lactamase activities. Here, the effect of amino acid substitutions in the possible omega-like loop region of NDM-5 has been elucidated. Overall, three substitution mutations near active site of NDM-5 were done, namely, E152A, S191A, and D223A and subsequently, the change in antimicrobial resistance was monitored upon expressing each mutant in a suitable host...
January 12, 2017: FEMS Microbiology Letters
Bihter Bayramoglu, David Toubiana, Osnat Gillor
Many studies have described the response of the facultative anaerobe, Escherichia coli, to anaerobic conditions, yet they all investigated free-living (planktonic) cells because attempts to cultivate anaerobic E. coli biofilm were mostly unsuccessful. We challenged these findings and cultivated E. coli strain MG1655 biofilm under both aerobic and anaerobic conditions, characterizing the mature biofilm architecture and global gene expression profile. We used RNA sequencing technology to compare stationary phase planktonic cells with mature biofilm, cultured with and without oxygen...
January 12, 2017: FEMS Microbiology Letters
Mariagrazia Pizza
No abstract text is available yet for this article.
January 12, 2017: FEMS Microbiology Letters
Evelien Gerits, Isolde Van der Massen, Katleen Vandamme, Kaat De Cremer, Katrijn De Brucker, Karin Thevissen, Bruno P A Cammue, Serge Beullens, Maarten Fauvart, Natalie Verstraeten, Jan Michiels
Oral infections are among the most common diseases worldwide. Many protocols for the prevention and treatment of oral infections have been described, yet no golden standard has been developed so far. The antiseptic chlorhexidine and antibiotics are often used in these treatment procedures. However, long-term use of chlorhexidine can lead to side effects and extensive use of antibiotics can promote the development of antibiotic-resistant bacteria, which in turn can compromise the effectiveness of the treatment...
January 12, 2017: FEMS Microbiology Letters
Manisha Kunadiya, Diane White, William A Dunstan, Giles E St, J Hardy, Vera Andjic, Treena I Burgess
Phytophthora cinnamomi is one of the world's most invasive plant pathogens affecting ornamental plants, horticultural crops and natural ecosystems. Accurate diagnosis is very important to determine the presence or absence of this pathogen in diseased and asymptomatic plants. In previous studies, P. cinnamomi species-specific primers were designed and tested using various polymerase chain reaction (PCR) techniques including conventional PCR, nested PCR, and quantitative real time PCR (qPCR). In all cases, the primers were stated to be highly specific and sensitive to P...
January 12, 2017: FEMS Microbiology Letters
Joseph Kreit
An overview on the microbial sterol catabolism is described with a focus on the catabolic step of the 3β-hydroxy-5-en structure. Cholesterol oxidase transforms this structure into the corresponding 3-keto-4-en feature, and thus initiates the sterol molecule catabolism. The oxidase has been found in a large number of microorganisms, especially in actinobacteria as species of Rhodococcus and Streptomyces Other actinobacteria as species of Mycobacterium and Nocardia possess NAD(P)-dependent dehydrogenase for this catabolic step...
January 12, 2017: FEMS Microbiology Letters
R Tareb, M Bernardeau, C Amiel, J P Vernoux
In this study, the potential of FTIR spectroscopy for assessing putative biochemical and structural differences between the two variants Rough (R) and Smooth (S) of Lactobacillus farciminis CNCM-I-3699, a pleomorphic strain, was investigated. The main differences observed were localized in the polysaccharide (1200-900 cm(-1)) and protein (1700-1500 cm(-1)) regions. Based on spectral information in these two spectral ranges, clustering resulted in a dendrogram that showed a clear discrimination between both morphotypes...
January 12, 2017: FEMS Microbiology Letters
Shuai Zhou, Yanmei Huang, Bingshao Liang, Hui Dong, Shuwen Yao, Yinshuang Chen, Yongqiang Xie, Yan Long, Sitang Gong, Zhenwen Zhou
PURPOSE: Previous epidemiologic studies have demonstrated an inverse association between Helicobacter pylori infection and the frequency of allergic asthma. The neutrophil-activating protein (NAP) of Helicobacter pylori has been identified as a modulator possessing anti-Th2 inflammation activity. Here, we sought to determine whether systemic or mucosal pre-administration of recombinant Helicobacter pylori NAP (rNAP) could prevent ovalbumin-induced allergic asthma in mice. METHODS: Mice were exposed to purified rNAP through intraperitoneal injection or inhalation and then sensitized with ovalbumin...
January 12, 2017: FEMS Microbiology Letters
Mariela E Srednik, Yannick D N Tremblay, Josée Labrie, Marie Archambault, Mario Jacques, Fernández Cirelli Alicia, Elida R Gentilini
Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNS isolated from cows with clinical and subclinical mastitis in Argentina from 2008-2014 were identified by PCR-RFLP using the gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation and Staphylococcus haemolyticus species formed the strongest biofilms...
January 12, 2017: FEMS Microbiology Letters
Utpal Bose, Catharine A Ortori, Sarir Sarmad, David A Barrett, Amitha K Hewavitharana, Mark P Hodson, John A Fuerst, P Nicholas Shaw
The structures of acyl homoserine lactone (AHL) compounds and their quantification was accomplished using an integrated liquid chromatography-mass spectrometry approach. The precursor and product ions, along with retention times of peaks, were searched against an in-house database of AHLs and structures confirmed by accurate mass and by comparison with authentic AHL standards. The two compounds, N-(3-oxodecanoyl)-L-homoserine lactone and N-(3-oxododecanoyl)-L-homoserine lactone were characterised and quantified in Salinispora sp...
January 12, 2017: FEMS Microbiology Letters
Katherine D'Amico, Melanie J Filiatrault
The plant pathogen Pseudomonas syringae accounts for substantial crop losses and is considered an important agricultural issue. To better manage disease in the field, it is important to have an understanding of the underlying genetic mechanisms that mediate virulence. There are a substantial number of genes in sequenced bacterial genomes, including P. syringae, that encode for conserved hypothetical proteins; some of these have been functionally characterized in other Pseudomonads and have been demonstrated to play important roles in disease...
January 10, 2017: FEMS Microbiology Letters
Julia M Kurth, Anja Schuster, Waldemar Seel, Stefanie Herresthal, Jörg Simon, Christiane Dahl
The diheme cytochromes c of the widespread TsdA family are bifunctional thiosulfate dehydrogenase/tetrathionate reductases. Here, biochemical information was collected about TsdA from the Epsilonproteobacterium Wolinella succinogenes (WsTsdA). The situation in W. succinogenes is unique since TsdA is closely associated with the unprecedented lipoprotein TsdC encoded immediately downstream of tsdA in the same direction of transcription. WsTsdA purified from Escherichia coli catalyzed both thiosulfate oxidation and tetrathionate reduction...
January 6, 2017: FEMS Microbiology Letters
Ronglin He, Chen Li, Jun Feng, Dongyuan Zhang
Hydrophobin proteins originate from filamentous fungi, which are able to self-assemble at water-air interfaces. Hydrophobins have multiple functions in fungal growth and development. In the present study, the function of the Trhfb3 gene encoding a class II hydrophobin was characterized in Trichoderma reesei The null mutant of Trhfb3 presented a wettable phenotype and a significantly reduced conidial production compared with the parent strain. The ΔTrhfb3 mutant also exhibited less biomass formation than the parent strain...
December 23, 2016: FEMS Microbiology Letters
Gopinath Viswanathan, Sangya Yadav, Shrilaxmi V Joshi, Tirumalai R Raghunand
FhaA is a forkhead associated domain containing protein, the depletion of which leads to accumulation of peptidoglycan (PG) precursors at the septum and poles in Mycobacterium smegmatis (M. smegmatis), by a mechanism undefined thus far. To elucidate its function, we constructed an fhaA (MSMEG_0035) knockout (ΔfhaA) strain in M. smegmatis and demonstrated that this gene is dispensable for in vitro growth. The mutant showed a short cell length phenotype due to a probable defect in cell elongation/cell wall synthesis, which was reversed by complementation with both M...
December 23, 2016: FEMS Microbiology Letters
Jing-Yi Zhao, Shu-Mei Zhao, Xiao-Dong Mu, Zijun Xiao
Three qnrS2-containing isolates of Pseudoalteromonas and Shewanella were collected from the seawater samples of Qingdao in China during 2014. They displayed resistance to ampicillin, ciprofloxacin, kanamycin, nalidixic acid and sulfamethoxazole. The qnrS2 genes were identified in the chromosomes of Pseudoalteromonas strain E8 and S16, and in a 140-kb plasmid in Shewanella strain S14, respectively. In addition, two copies of qnrS2 were identified in the strain E8. Sequence analyses revealed that there was an identical DNA segment located in the downstream of qnrS2 in strain S14 and E8, coding for a TetR transcriptional regulator, two putative integrases and a hypothetical protein...
December 23, 2016: FEMS Microbiology Letters
Robert Dolan
No abstract text is available yet for this article.
December 22, 2016: FEMS Microbiology Letters
Arnoud H M van Vliet
The human bacterial pathogen Helicobacter pylori has a highly variable genome, with significant allelic and sequence diversity between isolates and even within well-characterised strains, hampering comparative genomics of H. pylori In this study, pan-genome analysis has been used to identify lineage-specific genes of H. pylori A total of 346 H. pylori genomes spanning the hpAfrica1, hpAfrica2, hpAsia2, hpEurope, hspAmerind and hspEAsia multilocus sequence typing (MLST) lineages were searched for genes specifically over- or underrepresented in MLST lineages or associated with the cag pathogenicity island (PAI)...
December 22, 2016: FEMS Microbiology Letters
Panjaphorn Nimmanee, Emily W T Tam, Patrick C Y Woo, Pramote Vanittanakom, Nongnuch Vanittanakom
Stress-activated MAPK (SAPK) pathways are systems used to regulate the stress adaptation of most fungi. It has been shown that in Talaromyces marneffei (Penicillium marneffei), a pathogenic dimorphic fungus, the sakA gene is involved, not only in tolerance against oxidative and heat stresses, but also in playing a role in asexual development, yeast cell generation in vitro and survival inside macrophage cell lines. In this study, the role of the T. marneffei sakA gene on the nitrosative stress response, and the regulation of gene expression were investigated...
December 22, 2016: FEMS Microbiology Letters
Jean-Pierre Gratia
Subclonal analysis of spontaneous zygogenesis (Z-mating) products of Escherichia coli K12 was undertaken to grasp the extent of vertical and horizontal evolution in unstable strains expressing one parental or recombinant genome. Isolates were obtained following serial cultures or serial intercrosses. A high diversity of strains was obtained, among which some resumed the phenotype of the partners of the initial or subsequent Z-matings. When noncomplementing diploids are infected with a mixture of distinct temperate bacteriophages, lysogenization occurs at the expenses of the active chromosome only...
December 22, 2016: FEMS Microbiology Letters
Jose R López, Laura Lorenzo, Cinta Marcelino-Pozuelo, M Carmen Marin-Arjona, Jose I Navas
Pseudomonas baetica is a pathogen described in 2012 from wedge sole (Dicologoglossa cuneata). The aims of this study were to 1) determine the influence of temperature on its virulence; and 2) develop specific protocols for rapid diagnosis. Virulence assays carried out by bath using Senegalese sole fry, showed that virulence is strongly influenced by temperature. So, LD50 at 14°C was of 8.5×10(5) cfu/ml, meanwhile at 20°C no mortalities were recorded. On the other hand, the high mortality rates observed on virulence assays by intraperitoneal injection of 2...
December 22, 2016: FEMS Microbiology Letters
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