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FEMS Microbiology Letters

Anna Koza, Anna Kusmierska, Kimberley McLaughlin, Olena Moshynets, Andrew J Spiers
Combined experimental evolutionary and molecular biology approaches have been used to investigate the adaptive radiation of Pseudomonas fluorescens SBW25 in static microcosms leading to the colonisation of the air-liquid interface by biofilm-forming mutants such as the Wrinkly Spreader. In these microcosms, the ecosystem engineering of the early wild-type colonists establish the niche space for subsequent WS evolution and colonisation. Random WS mutations occurring in the developing population that de-regulate diguanylate cyclases and c-di-GMP homeostasis result in cellulose-based biofilms at the air-liquid interface...
May 23, 2017: FEMS Microbiology Letters
Guang-Yu Wang, Ming Li, Fang Ma, Hu-Hu Wang, Xing-Lian Xu, Guang-Hong Zhou
Pseudomonas spp., in particular Pseudomonas fragi, are dominant in aerobically stored chilled meats. This work isolated P. fragi isolates from spoiled chicken and investigated the effect of modified atmosphere packaging (MAP, CO2/N2 30%/70%) on physicochemical properties of P. fragi isolates compared to the corresponding controls (air). A total of 6 P. fragi isolates were isolated from aerobically stored spoiled chicken. MAP inhibited the growth of the isolates primarily in the exponential phase without inducing cell death and weakened the isolate's ability to form biofilms...
May 22, 2017: FEMS Microbiology Letters
Beatrix Fahnert
No abstract text is available yet for this article.
May 18, 2017: FEMS Microbiology Letters
Shelby E Gantt, Susanna López-Legentil, Patrick M Erwin
Marine sponges have been shown to harbor diverse microbial symbiont communities that play key roles in host functioning, yet little is known about how anthropogenic disturbances impact sponge-microbe interactions. The Mediterranean sponge Crambe crambe is known to accumulate heavy metals in polluted harbors. In this study, we investigated whether the microbiome of C. crambe differed between sponges inhabiting a polluted harbor in Blanes (Spain) and a nearby (< 1 km) natural environment. Triplicate sponge and ambient seawater samples were collected from each site and the microbial composition of each sample was determined by 16S rRNA gene sequence analysis (Illumina Hi-Seq platform)...
May 18, 2017: FEMS Microbiology Letters
Mayumi Hatakeyama, Takuya Kitaoka, Hirofumi Ichinose
Aspergillus oryzae cytochrome P450 monooxygenase (CYP57B3) is capable of catalyzing hydroxylation of genistein to produce 3΄-hydroxygenistein. Because hydroxylated derivatives of genistein, including 3΄-hydroxygenistein, exhibit various pharmacological activities, CYP57B3 would potentially be useful as a biocatalyst in the pharmaceutical field. We therefore performed random mutagenesis of CYP57B3 to improve its catalytic activities for genistein. Random mutations were introduced by error-prone PCR into CYP57B3, resulting in construction of a library of mutants...
May 18, 2017: FEMS Microbiology Letters
V I Francis, E C Stevenson, S L Porter
Pseudomonas aeruginosa is a versatile opportunistic pathogen capable of infecting a broad range of hosts, in addition to thriving in a broad range of environmental conditions outside of hosts. With this versatility comes the need to tightly regulate its genome to optimise its gene expression and behaviour to the prevailing conditions. Two-component systems (TCSs) comprising sensor kinases and response regulators, play a major role in this regulation. This minireview discusses the growing number of two-component systems that have been implicated in the virulence of Pseudomonas aeruginosa, with a special focus on the emerging theme of multikinase networks, which are networks comprising multiple sensor kinases working together, sensing and integrating multiple signals to decide upon the best response...
May 16, 2017: FEMS Microbiology Letters
Jeff Errington
No abstract text is available yet for this article.
May 16, 2017: FEMS Microbiology Letters
Denis Mariat, Véronique Robert, Philippe Langella, Jean-Marc Chatel
Lactic acid bacteria as Lactococcus lactis are used as vector for protein but also DNA delivery into intestinal cells in vitro and in vivo. For the plasmid delivery strategy, the plasmid copy number per bacteria (PCN) is thus of great importance. The aim of this paper is to determine the physiological conditions when PCN is the highest in the bacteria. PCN was characterized by qPCR in five different recombinant Lactococcus lactis strains, containing one (mono-) or two different plasmids (biplasmidic), at exponential or stationary phase...
May 15, 2017: FEMS Microbiology Letters
Fernando H Martins, Roberto Nepomuceno, Roxane M F Piazza, Waldir P Elias
Enteropathogenic Escherichia coli (EPEC) strains employ the type III secretion system (T3SS) effector Tir to induce actin cytoskeletal rearrangements. While some EPEC require tyrosine phosphorylation (Y-P) of Tir to trigger actin assembling, certain strains whose Tir is not tyrosine phosphorylated utilize the T3SS effector Tir-cytoskeleton coupling protein (TccP/TccP2) for efficient actin polymerization. The presence of tccP/tccP2 in typical EPEC belonging to distinct evolutionary lineages is well established but, in contrast, little is known about the distribution of these genes in atypical EPEC (aEPEC) showing distinct phylogenetic background...
May 15, 2017: FEMS Microbiology Letters
Yuji Aiba, Hiroki Ishikawa, Masayoshi Tokunaga, Yasuhiko Komatsu
Some strains of lactic acid bacteria are reported to inhibit the growth of Helicobacter pylori (H. pylori) and proposed to be useful to support so-called triple therapy for H. pylori. Although most strains must be alive to exert their anti-H. pylori activity, some lactobacilli strains are effective even when dead. One possible underlying mechanism of such an activity of non-living lactobacilli is reportedly co-aggregation with H. pylori. In the present study, we found that a non-living heat-killed form of Lactobacillus johnsonii No...
May 15, 2017: FEMS Microbiology Letters
Wolfgang Kneifel, Stephen Forsythe
No abstract text is available yet for this article.
May 15, 2017: FEMS Microbiology Letters
Amy Siegesmund
Student success is too often challenged by a lack of metacognition and ability to self-regulate learning. This commentary argues that the use of self-assessment to increase student metacognition positively impacts student learning and self-regulation. In addition, several strategies for incorporating self-assessment will be presented.
May 11, 2017: FEMS Microbiology Letters
Boriana Marintcheva
Virtual virus is a semester-long interdisciplinary project offered as part of upper level elective course in Virology. Students are challenged to apply key concepts from multiple biological sub-disciplines to 'synthesize' a plausible virtual virus. The project is executed as a scaffolded series of hands-on sessions and mini-projects which are integrated into continuous story leading to mock conference presentation and comprehensive report modeling article publication. It complements classroom instruction helping students to meet overarching learning targets traditionally associated undergraduate virology courses such as: viral structure and function, mode of viral propagation and flow of genetic information, and virus/host interactions on the cellular and organismal level...
May 11, 2017: FEMS Microbiology Letters
Lise Goetghebuer, Pierre Servais, Isabelle George
Microbial communities play a key role in water self-purification. They are primary drivers of biogenic element cycles and ecosystem processes. However, these communities remain largely uncharacterized. In order to understand the diversity-heterotrophic activity relationship facing sole carbon sources, we assembled a synthetic community composed of 20 'typical' freshwater bacterial species mainly isolated from the Zenne River (Belgium). The carbon source utilization profiles of each individual strain and of the mixed community were measured in Biolog Phenotype MicroArrays PM1 and PM2A microplates that allowed testing 190 different carbon sources...
May 11, 2017: FEMS Microbiology Letters
Mia M Howard, Terrence H Bell, Jenny Kao-Kniffin
We show that choice of soil microbiome transfer method, i.e. direct soil transfers and a common soil wash procedure, dramatically influences the microbiome that develops in a new environment, using high-throughput amplicon sequencing of 16S rRNA genes and the fungal ITS region. After three weeks of incubation in commercial potting mix, microbiomes were most similar to the source soil when a greater volume of initial soil was transferred (5% v/v transfer), and least similar when using a soil wash. Abundant operational taxonomic units (OTUs) were substantially affected by transfer method, suggesting that compounds transferred from the source soil, shifts in biotic interactions, or both, play an important role in their success...
May 4, 2017: FEMS Microbiology Letters
Wenyu Gu, Muhammad Farhan Ul Haque, Jeremy D Semrau
Methanotrophs, or methane-oxidizing bacteria exhibit a unique 'copper-switch' where expression of two forms of methane monooxygenase is controlled by the availability of copper. In the absence of copper, a cytoplasmic or soluble methane monooxygenase (sMMO) is expressed. In the presence of copper a membrane-bound or particulate methane monooxygenase (pMMO) is expressed. These two forms of MMO have very different properties, and elucidation of the basis of the copper-switch is of significant interest as methanotrophs are becoming increasingly popular for the valorization of methane...
May 4, 2017: FEMS Microbiology Letters
Na Zhou, Shuai Zhao, Chang-Yan Tian
Utilization of rhizobacteria that have associated with plant roots in harsh environments could be a feasible strategy to deal with limits to agricultural production caused by soil salinity. Halophytes occur naturally in high-salt environments, and their roots may be associated with promising microbial candidates for promoting growth and salt tolerance in crops. This study aimed to isolate efficient halotolerant plant-growth-promoting rhizobacterial strains from halophytes and evaluate their activity and effects on sugar beet (Beta vulgaris L...
April 29, 2017: FEMS Microbiology Letters
Shiwen Zhuang, Diana Chinyere Anyaogu, Takeshi Kasama, Mhairi Workman, Uffe Hasbro Mortensen, Timothy John Hobley
We report effects of dissolved oxygen (DO) concentration and iron addition on gene expression of Magnetospirillum gryphiswaldense MSR-1 cells during fermentations, focusing on 0.25-24 h after iron addition. The DO was strictly controlled at 0.5% or 5% O2, and compared with aerobic condition. Uptake of iron (and formation of magnetosomes) was only observed in the 0.5% O2 condition where there was little difference in cell growth and carbon consumption compared to the 5% O2 condition. Quantitative reverse transcription PCR analysis showed a rapid (within 0...
April 19, 2017: FEMS Microbiology Letters
Yuki Maeda, Chun-Yi Lin, Yojiro Ishida, Masayori Inouye, Yoshihiro Yamaguchi, Sangita Phadtare
Reminiscent of eukaryotic apoptotic programmed cell death, bacteria also contain a large number of suicide genes, which are in general co-expressed with their cognate antitoxic genes. These systems called the toxin-antitoxin (TA) systems are associated with cellular dormancy, and play major roles in biofilm formation and persistent multi-drug resistance of many human pathogens. In recent years, the study on TA system toxins has become a hot topic due to the health implications of these toxins by virtue of their role in bacterial pathogenicity...
April 19, 2017: FEMS Microbiology Letters
Jon James
This commentary describes some of the current challenges for science education in the UK and how an EU educational project (PERFORM) is seeking to use performing arts to engage young people with science, its values, and the processes of research.
April 12, 2017: FEMS Microbiology Letters
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