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FEMS Microbiology Letters

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https://www.readbyqxmd.com/read/30423131/the-unbearable-ease-of-expression-how-avoidance-of-spurious-transcription-can-shape-g-c-content-in-bacterial-genomes
#1
Uri Gophna
No abstract text is available yet for this article.
November 13, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30423115/scientific-communication-strategies-of-microbiologists-in-the-era-of-social-media
#2
Stefano Romano, Rebecca Ansorge
Over the last decades, the world of communication underwent drastic changes, and Internet and social media emerged as essential vehicles for exchanging information. Following these trends, it is important that scientists adapt to changes and adopt optimal strategies to communicate with colleagues, lay people, and institutions. We conducted an on-line survey to investigate the communication strategies of microbiologists and their colleagues from other disciplines. We collected data from 527 scholars from 57 countries, with ∼42% of them being microbiologists...
November 12, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30418577/a-rapid-and-non-pathogenic-assay-for-association-of-mycobacterium-tuberculosis-gyrba-mutations-and-fluoroquinolone-resistance-using-recombinant-mycobacterium-smegmatis
#3
Mitsunori Yoshida, Noboru Nakata, Yuji Miyamoto, Hanako Fukano, Manabu Ato, Yoshihiko Hoshino
We developed a method involving recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG) and recombinant Mycobacterium smegmatis to determine which mutations in Mycobacterium tuberculosis (Mtb) gyrBA are associated with fluoroquinolone (FQ) resistance. The minimal inhibitory concentration (MIC) for FQ for recombinant strains with wild-type Mtb gyrBA was equivalent to that for strains with intrinsic gyrBA. Among 27 gyrBA mutations, the fold-changes in FQ MIC for M. smegmatis and M. bovis BCG backgrounds were comparable and were in part equivalent to those previously reported for recombinant Mtb strains...
November 10, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30398655/erg6-gene-is-essential-for-stress-adaptation-in-kluyveromyces-lactis
#4
Alexandra Konecna, Nora Toth Hervay, Alexandra Bencova, Marcela Morvova, Libusa Sikurova, Iva Jancikova, Dana Gaskova, Yvetta Gbelska
We investigated the effect of Kluyveromyces lactis ERG6 gene deletion on plasma membrane function and showed increased susceptibility of mutant cells to salt stress, cationic drugs and weak organic acids. Contrary to Saccharomyces cerevisiae, Klerg6 mutant cells exhibited increased tolerance to tunicamycin. The content of cell wall polysacharides did not significantly vary between wild-type and mutant cells. Although the expression of the NAD+- dependent glycerol 3-phosphate dehydrogenase (KlGPD1) in the Klerg6 mutant cells was only half of that in the parental strain, it was induced in the presence of calcofluor white...
November 6, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30376063/a-cohabiting-bacterium-alters-the-spectrum-of-short-rnas-secreted-by-escherichia-coli
#5
Olga V Alikina, Olga A Glazunova, Alexandr A Bykov, Sergey S Kiselev, Maria N Tutukina, Konstantin S Shavkunov, Olga N Ozoline
Recently, it has been found that bacteria secrete short RNAs able to affect gene expression in eukaryotic cells, while certain mammalian microRNAs 'shape the gut microbiome' altering bacterial transcriptome. The involvement of bacterial RNAs in communication with other bacteria is also expected, but has not been documented yet. Here, we compared the fractions of extremely short (12-22 nucleotides) RNAs secreted by Escherichia coli grown in a pure culture and jointly with bacteria of the Paenibacillus genus...
October 29, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30364948/expression-of-lactobacillus-plantarum-kw30-gcc-genes-correlates-with-the-production-of-glycocin-f-in-late-log-phase
#6
Soyeon Ahn, Judith Stepper, Trevor S Loo, Sean W Bisset, Mark L Patchett, Gillian E Norris
Antibacterial compounds known as bacteriocins are microbial inventions designed to reduce competition for limited resources by inhibiting the growth of closely related bacteria. Glycocin F (GccF) is an unusually di-glycosylated bacteriocin produced in a lactic acid bacterium (LAB), Lactobacillus plantarum KW30 that has been shown to be resistant to extreme conditions. It is bacteriostatic rather than bactericidal, and all its post-translational modifications (a pair of nested disulfide bonds, and O-linked and S-linked N-acetylglucosamines) are required for full activity...
October 25, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30357328/droplet-based-microfluidics-as-a-future-tool-for-strain-improvement-in-lactic-acid-bacteria
#7
Jun Chen, Mike Vestergaard, Jing Shen, Christian Solem, Martin Dufva, Peter Ruhdal Jensen
Strain development is frequently used to improve the performance and functionality of industrially important microbes. As traditional mutagenesis screen is especially utilized by the food industry to improve strains used in food fermentation, high-throughput and cost-effective screening tools are important in mutant selection. The emerging droplet-based microfluidics technology miniaturizes the volume for cell cultivation and phenotype interrogation down to the pico-liter scales, which facilitates screening of microbes for improved phenotypical properties tremendously...
October 24, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30346523/research-letter-cross-reactivity-of-latex-agglutination-assay-complicates-the-identification-of-burkholderia-pseudomallei-from-soil
#8
Jirarat Songsri, Yuta Kinoshita, Wiyada Kwanhian, Sueptrakool Wisessombat, Jitbanjong Tangpong, Md Siddiqur Raman Khan, Apichai Tuanyok
The monoclonal antibody-based latex agglutination tests targeting a high molecular weight exopolysaccharide antigen of Burkholderia pseudomallei are commercially available. The tests are primarily used in routine diagnosis of melioidosis in major hospitals in Thailand and some endemic countries. Being a rapid test, this technique was employed as a presumptive test to identify colonies of B. pseudomallei among many others grown from soil specimens collected from southern Thailand. Cross-reactivity of these tests with other soil bacteria was a concern since it complicated the identification of B...
October 22, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30346519/preparation-tips-for-academic-interviews
#9
Bob Dolan
No abstract text is available yet for this article.
October 22, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30346516/genome-analysis-of-lactic-acid-bacterial-strains-selected-as-potential-starters-for-traditional-slovakian-bryndza-cheese
#10
Barbora Markusková, Aneta Lichvariková, Tomáš Szemes, Janka Korenová, Tomáš Kuchta, Hana Drahovská
Genomes of 21 strains of lactic acid bacteria isolated from Slovakian traditional cheeses were sequenced on an Illumina MiSeq platform. Subsequently, they were analysed regarding taxonomic classification, presence of genes encoding defence systems, antibiotic resistance and production of biogenic amines. Thirteen strains were found to carry genes encoding at least one bacteriocin, 18 carried genes encoding at least one restriction-modification system, all strains carried 1-6 prophages and 9 strains had CRISPR-Cas systems...
October 22, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30346510/metabolomics-approach-used-for-understanding-temperature-related-pectinase-activity-in-bacillus-licheniformis-dy2
#11
Yi Guan, Di Yin, Xi Du, Xiuyun Ye
Pectinases are enzymes that catalyze pectin degradation. There is a global demand for pectinases because of their wide utility and catalytic efficiency. Optimization of the fermentation process to increase the pectolytic enzyme activity is generally practiced to lower process costs, but whether temperature influences the metabolome, enhancing pectinase activity, is not known. Here, we developed a metabolomics approach to explore it. The activity of P-DY2 pectinase produced by Bacillus licheniformis DY2 was higher in cells grown at 30°C than those grown at 37 °C...
October 22, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30307512/prediction-and-identification-of-novel-srnas-involved-in-agrobacterium-strains-by-integrated-genome-wide-and-transcriptome-based-methods
#12
Ilamathi Raja, Vikram Kumar, Hariharan Sabapathy, Manoharan Kumariah, Kasthuri Rajendran, Jebasingh Tennyson
sRNAs are a class of gene regulators in bacteria, playing a central role in its response to environmental changes. Bioinformatic prediction facilitates the identification of sRNAs expressed at different conditions. We propose a novel method of prediction of sRNAs from the genome of Agrobacterium based on PWM matrix of conditional sigma factors. sRNAs predicted from the genome are integrated with the virulence specific transcriptome data to identify putative sRNAs that are over expressed during Agrobacterial virulence induction...
October 11, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30304507/modulation-of-quorum-sensing-controlled-virulence-factors-in-chromobacterium-violaceum-by-selective-amino-acids
#13
Leena Champalal, Udhaya Santhosh Kumar, Nagasathya Krishnan, Baskaralingam Vaseeharan, Vairamani Mariappanadar, Raman Pachaiappan
Bacterial pathogenesis regulation requires N-acyl homoserine lactone (AHL)-based quorum sensing (QS). The main objective of this study was to assess the anti-quorum sensing and anti-biofilm potential of five different amino acids namely serine, aspartic acid, lysine, leucine, and tryptophan. The selected amino acids were assessed for their ability in inhibiting QS activity such as exopolysaccharide (EPS) production, biofilm formation, pigment production in Chromobacterium violaceum, and swarming motility. At 0...
October 10, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30299471/streptomyces-protein-secretion-and-its-application-in-biotechnology
#14
Mohamed Belal Hamed, Jozef Anné, Spyridoula Karamanou, Anastassios Economou
Bacteria are of tremendous importance in the pharma- and bio-industry as producers of a broad range of economically interesting metabolites and proteins. Gram-positive bacteria are valuable hosts for the production of heterologous proteins for obvious reasons. Contrary to Gram-negative bacteria, Gram-positive bacteria release their secreted proteins immediately into the spent culture broth as they are not hindered by an outer membrane. Secretory protein production also avoids the formation of inclusion bodies, hence facilitating downstream processing...
October 8, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30295732/expression-dynamics-of-the-poly-%C3%AE-glutamic-acid-biosynthesis-genes-of-bacillus-subtilis-in-response-to-glucose-and-glutamic-acid-a-pilot-study
#15
Deepika Pandey Tiwari, Poonam Mishra Chatterjee, Harish Rotti, Bipin Chand, Ritu Raval, Ashok Kumar Dubey
Poly-γ-glutamic acid (PGA) is biosynthesized by various Bacillus species through PGA synthetase, encoded by PGA operon comprising of ywsC and ywtABC genes. Due to the minimal available knowledge, understanding the expression pattern of PGA operon genes is pivotal. In this study, the effect of glucose and glutamic acid on global gene expression profile of Bacillus subtilis Natto3 was investigated using high throughput microarray, with an emphasis on PGA operon and genes influencing PGA production. Two treatment groups (set1-in presence of glutamic acid and set2-in presence of glutamic acid+glucose) were analyzed against control, (in presence of glucose)...
October 5, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30289449/the-screening-and-identification-of-the-biological-control-fungi-chaetomium-spp-against-wheat-common-root-rot
#16
Hai-Mei Yue, Bing Wang, Wen-Feng Gong
Bipolaris sorokiniana is a soil-borne fungal pathogen, causing common root rot in wheat, and is difficult to control through chemical and agricultural means. Chaetomium spp. have been documented as potential biological control agents for plant diseases. In this study, seven Chaetomium strains were screened for their abilities to control Bipolaris sorokiniana on agar plates. Strain 22-10 significantly inhibited the growth of B. sorokiniana on PDA plates up to 66.7%. Its filtrate of liquid culture also inhibited the mycelial growth of B...
October 4, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30265303/genome-mining-for-the-search-and-discovery-of-bioactive-compounds-the-streptomyces-paradigm
#17
A C Ward, Nee Allenby
The need for new antimicrobials is indisputable. The flight from natural products in drug discovery was unfortunate. However, the revolution that is genome mining, enabled by the explosion in sequencing technology, is a cause for hope. Nevertheless, renewed search and discovery is still a challenge. We explore novel metabolite diversity and the challenges in Streptomyces. Estimating novel bioactive metabolites remaining to be discovered is an important driver for future investment. Frequent re-discovery of known natural products was a major factor in big pharma exiting search and discovery and remains a reality...
September 27, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30256945/basics-of-genome-scale-metabolic-modeling-and-applications-on-c1-utilization
#18
Ilyas Kabimoldayev, Anh Duc Nguyen, Laurence Yang, Sunghoon Park, Eun Yeol Lee, Donghyuk Kim
It is fundamental to understand the relationship between genotype and phenotype in biology. This requires comprehensive knowledge of metabolic pathways, genetic information and well-defined mathematic modeling. Integration of knowledge on metabolism with mathematical modeling results in genome-scale metabolic models which have proven useful to investigate bacterial metabolism and to engineer bacterial strains capable of producing value-added biochemical. Single carbon substrates such as methane and carbon monoxide have drawn interests and they assumed one of next-generation feedstocks because of their high abundance and low price...
September 25, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30252037/a-novel-sucrose-based-expression-system-for-heterologous-proteins-expression-in-thermotolerant-methylotrophic-yeast-ogataea-thermomethanolica
#19
Aekkachai Puseenam, Kanokarn Kocharin, Sutipa Tanapongpipat, Lily Eurwilaichitr, Supawadee Ingsriswang, Niran Roongsawang
The thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC656 is a potential host for heterologous protein expression. In this study, a novel expression system was developed for O. thermomethanolica based on the maltase (mal) gene promoter from this organism. The OtMal promoter function was tested for expression of fungal enzymes as reporter genes. Measurement of xylanase reporter enzyme activity showed that the OtMal promoter was repressed during growth on glucose and was activated by sucrose. When sucrose was used as a carbon source, the OtMal promoter was approximately twice as strong as the constitutive OtGAP promoter...
September 24, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30239714/antirestriction-activities-of-klca-rp4-and-ardb-r64-proteins
#20
Ignatiy I Goryanin, Anna A Kudryavtseva, Vladimir P Balabanov, Valentina S Biryukova, Ilya V Manukhov, Gennadii B Zavilgelsky
Antirestriction proteins of the ArdB group (ArdB, KlcA) specifically inhibit restriction (endonuclease) activity of restriction-modification (RM) type I systems. Anti-restriction activity of KlcA and ArdB, encoded in transmissible plasmids RP4 (IncPα) and R64 (IncI1), respectively, has been determined. We show that the protein KlcA (RP4), an amino acid sequence identical to that of the protein KlcA (RK2), inhibits the activity of EcoKI when the klcA gene is located on the plasmid under the control of strong promoter...
September 18, 2018: FEMS Microbiology Letters
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