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FEMS Microbiology Letters

Feifei Cheng, Anzhou Ma, Xuliang Zhuang, Xiaojia He, Guoqiang Zhuang
The phytopathogen Pseudomonas syringae pv. tabaci 11528 (P. syringae 11528), causing wild-fire disease in soybean and tobacco plants, processes PsyI-PsyR quorum sensing (QS) system, in which PsyI is the N-(3-oxo-hexanoyl)-homoserine lactone (3OC6-HSL) synthase. In comparison to P. syringae 11528 AHL-deficient mutant, 845 3OC6-HSL-dependent genes were identified using RNA sequencing (RNA-seq) in the AHL-deficient mutant grown with exogenous 3OC6-HSL in the transition from the exponential to the stationary phase, and many of them were associated with virulence, which were negatively regulated...
November 17, 2016: FEMS Microbiology Letters
Sadegh Feizollahzadeh, Hossein Khanahmad, Ilnaz Rahimmanesh, Mazdak Ganjalikhani-Hakemi, Alireza Andalib, Mohammad Hossein Sanei, Abbas Rezaei
Food grade bacterium Lactococcus lactis is increasingly used for heterologous protein expression in therapeutic and industrial applications. The ability of L. lactis to secrete biologically active cytokines may be used for the generation of therapeutic cytokines. Interleukin (IL)-18 enhances the immune response, especially on mucosal surfaces, emphasizing its therapeutic potential. However, it is produced as an inactive precursor and has to be enzymatically cleaved for maturation. We genetically manipulated L...
November 17, 2016: FEMS Microbiology Letters
Mark R Hartman, Kristin T Harrington, Candice M Etson, Matthew B Fierman, Donna K Slonim, David R Walt
Sequencing and bioinformatics technologies have advanced rapidly in recent years, driven largely by developments in next-generation sequencing (NGS) technology. Given the increasing importance of these advances, there is a growing need to incorporate concepts and practices relating to NGS into undergraduate and high school science curricula. We believe that direct access to sequencing and bioinformatics will improve the ability of students to understand the information obtained through these increasingly ubiquitous research tools...
November 16, 2016: FEMS Microbiology Letters
Frederico Schmitt Kremer, Marcus Redü Eslabão, Odir Antônio Dellagostin, Luciano da Silva Pinto
Next-Generation Sequencing (NGS) has significantly reduced the cost of genome sequencing projects, resulting in an expressive increase in the availability of genomic data in public databases. The cheaper and easier is to sequence new genomes, the more accurate the annotation steps have to be to avoid both the loss of information and the accumulation of erroneous features that may affect the accuracy of further analysis. In the case of bacteria genomes, a range of web annotation software has been developed; however, many applications have yet to incorporate the steps required to improve their result, including the removal of false-positive/spurious and a more complete identification of non-coding features...
November 16, 2016: FEMS Microbiology Letters
Sarah Maria Hücker, Svenja Simon, Siegfried Scherer, Klaus Neuhaus
The enteric pathogen Escherichia coli O157:H7 Sakai (EHEC) is able to grow at lower temperatures compared to commensal E. coli Growth at environmental conditions displays complex challenges different to those in a host. EHEC was grown at 37°C (control) and at 14°C with 4% NaCl, a combination of cold and osmotic stress as present in the food chain. Comparison of RNAseq and RIBOseq data provided a snap shot of ongoing transcription and translation, differentiating transcriptional and post-transcriptional gene regulation, respectively...
November 16, 2016: FEMS Microbiology Letters
João Carlos Campanharo, Anna Maria Kielak, Tereza Cristina Luque Castellane, Eiko Eurya Kuramae, Eliana Gertrudes de Macedo Lemos
The members of subdivision 1 Acidobacterium were grown at different pH values in a new medium formulation named PSYL 5 includes sucrose, as a carbon source and other compounds (such as KH2PO4 and MgSO47H2O). Growth rate was nearly constant at pH 5 and declined at pH 3-4 and 6-7. However, it was found that the effects involving good C/N ratios and pH on the growth of members of subdivision 1 Acidobacterium were significant, and the strongest effect was given by this conditions at pH 5. In additional, incubation results of 48, 72, 96 and 120 h were relatively shorter as compared to other media earlier described for members of subdivision 1 of the phylum Acidobacteria on solid laboratory media...
November 2, 2016: FEMS Microbiology Letters
Jingjing Sun, Xiaojian Gao, Jiang Qun, Xuedi Du, Keran Bi, Xiaojun Zhang, Li Lin
This study aimed to evaluate the survival and gene expression of Vibrio harveyi under starvation conditions. The microcosms V. harveyi were incubated in sterilized seawater for four weeks at room temperature. Overall, the cell numeration declined rapidly about 10(3) CFU/ ml during starvation, with a tiny rebound at day twenty-one. Scanning electron microscopy revealed rod-shaped cells became sphere with a rippled cell surface. By polymerase chain reaction (PCR) assay, nine genes, named luxR, toxR, vhhB, flaA, topA, fur, rpoS, mreB, ftsZ were detected in the non-starvedi cells...
November 2, 2016: FEMS Microbiology Letters
Xiaohan Zhu, Tengsheng Zhou, Liqiong Chen, Shiqin Zheng, Shaohua Chen, Dongmei Zhang, Guangpu Li, Zonghua Wang
Asexual development of phytopathogenic fungi such as Magnaporthe oryzae involves morphological changes that require spatiotemporal regulation of polarized growth. ADP-ribosylation factor 6 (Arf6) is a small GTPase known to regulate membrane trafficking and organization of the actin cytoskeleton at the cell surface, and consequently has an impact on cell morphology and polarity. In this study, we have functionally characterized the Arf6 homolog in M. oryzae, showing that △arf6 exhibits hyper-branching at hyphal tips and morphologically abnormal conidia as a result of defective polarized growth...
November 2, 2016: FEMS Microbiology Letters
Rahul Nandre, Xiaosai Ruan, Qiangde Duan, Weiping Zhang
Enterotoxigenic Escherichia coli (ETEC) bacteria producing heat-stable toxin (STa) and/or heat-labile toxin (LT) are among top causes of children's diarrhea and travelers' diarrhea. Currently no vaccines are available for ETEC associated diarrhea. A major challenge in developing ETEC vaccines is the inability to stimulate protective antibodies against the key STa toxin which is potently toxic and also poorly immunogenic. A recent study suggested toxoid fusion 3xSTaN12S-dmLT, which consists of a monomer LT toxoid (LTR192G/L211A) and three copies of STa toxoid STaN12S, may represent an optimal immunogen inducing neutralizing antibodies against STa toxin [IAI 2014, 82(5):1823-32]...
November 2, 2016: FEMS Microbiology Letters
Verena Nowroth Lisa Marquart, Dieter Jendrossek
The culturability of Escherichia coli, Ralstonia eutropha and of Bacillus subtilis after incubation in phosphate-buffered saline at either 5°C or 30°C was determined. Culturability of B. subtilis hardly depended on temperature. Culturability of E. coli rapidly decreased at 30°C but remained almost constant at 5°C. In contrast, culturability of R. eutropha decreased by three orders of magnitude at 5°C within 24 hours but only moderately decreased (one order of magnitude) at 30°C. Remarkably, prolonged incubation of R...
November 2, 2016: FEMS Microbiology Letters
Jacqueline Marshall, Yannick Rossez, Geoffrey Mainda, David L Gally, Tim Daniell, Nicola Holden
Type 1 fimbriae (T1F) are well characterised cell surface organelles expressed by Escherichia coli and required for adherence to mannosylated host tissue. They satisfy molecular Koch's postulates as a virulence determinant and a host-adapted role has been reinforced by reports that T1F expression is repressed at sub-mammalian temperatures. Analysis of a group of 136 environmental and animal E. coli isolates that express T1F at 37 °C showed that 28% are also capable of expression at 20 °C, in a phase variable manner...
November 2, 2016: FEMS Microbiology Letters
Marcio L Rodrigues, Leonardo Nimrichter, Radames J B Cordero
Science is international by nature. Scientific exchange and international mobility are essential for training young scientists in general and international collaboration has been directly linked to high-quality science and innovation. In this article, we present evidence showing that international mobility has a direct and beneficial impact on scientific discovery, career development, and cultural maturity, especially for students and young scientists.
October 25, 2016: FEMS Microbiology Letters
Elizabeth C Sargent, Andrew Hitchcock, S Andreas Johansson, Rebecca Langlois, C Mark Moore, Julie LaRoche, Alex J Poulton, Thomas S Bibby
Polyploidy is a well-described trait in some prokaryotic organisms; however, it is unusual in marine microbes from oligotrophic environments, which typically display a tendency towards genome streamlining. The biogeochemically significant diazotrophic cyanobacterium Trichodesmium is a potential exception. With a relatively large genome and a comparatively high proportion of non-protein-coding DNA Trichodesmium appears to allocate relatively more resources to genetic material than closely related organisms and microbes within the same environment...
October 25, 2016: FEMS Microbiology Letters
Yuriya Yukioka, Tsukiko Tanahashi, Keisuke Shida, Haruka Oguchi, Shota Ogawa, Chiaki Saito, Shunsuke Yajima, Shinsaku Ito, Kanju Ohsawa, Hirofumi Shoun, Yasuyuki Sasaki
Although nitric oxide (NO) is an important signaling molecule in bacteria and higher organisms, excessive intracellular NO is highly reactive and dangerous. Therefore, living cells need strict regulation systems for cellular NO homeostasis. Recently, we discovered that Streptomyces coelicolor A3(2) retains the nitrogen oxide cycle (NO3(-)→NO2(-)→NO→NO3(-)) and nitrite removal system. The nitrogen oxide cycle regulates cellular NO levels, thereby controlling secondary metabolism initiation (red-pigmented antibiotic, RED production) and morphological differentiation...
October 18, 2016: FEMS Microbiology Letters
Hongmei Jiao, Hui Yang, Dan Zhao, Li He, Jin Chen, Guocai Li
Humans health has been seriously endangered by highly prevalent salmonellosis and multidrug resistant Salmonella strains. Current vaccines suffer from variable immune-protective effects, so more effective ones are in need to control Salmonella infection : Bacterial ghosts have been produced by the expression of lysis gene E from bacteriophage PhiX174 and can be filled with considerable exogenous substances such as DNA or drugs as a novel platform. In this study, Salmonella enteritidis (SE) ghosts were developed and loaded with Neisseria gonorrhoeae porin B (porB) to construct a novel inactive vaccine...
October 18, 2016: FEMS Microbiology Letters
Karen Michael, David No, Jennifer Dankoff, Kyoyi Lee, Elisabeth Lara-Crawford, Marilyn C Roberts
Clostridium difficile is both a hospital and community acquired pathogen. The current study determined if C. difficile could be cultured from clinical laundry facility surfaces. A total of 240 surface samples were collected from dirty areas (n = 120), which handle soiled clinical linens, and from clean areas (n = 120), which process and fold the clean linens, within the University of Washington Consolidated Laundry facility in 2015. Sampling was done four times over the course of one year. The dirty area was significantly more contaminated than the clean area (21% vs 2%, p<0...
October 14, 2016: FEMS Microbiology Letters
Yong Yang, Hua Gu, Xiaofeng Yu, Li Zhan, Jiancai Chen, Yun Luo, Yunyi Zhang, Yanjun Zhang, Yiyu Lu, Jianmin Jiang, Lingling Mei
Emetic toxin producing Bacillus cereus (emetic B. cereus) is the third member of B. cereus group whose toxins are encoded by megaplasmids, beside anthrax and insecticidal toxins of B. anthracis and B. thuringiensis respectively. 18 emetic isolates collected from food poisoning events, clinical and non-random food samples in Zhejiang province of China, were analyzed by plasmid screening, pulse field gel electrophoresis (PFGE), multi-locus sequence typing (MLST), as well as toxic gene identification to investigate their genotypic diversity...
October 14, 2016: FEMS Microbiology Letters
Robert Dolan
No abstract text is available yet for this article.
October 14, 2016: FEMS Microbiology Letters
David S Senchina
Infectious diseases are potential catalysts for exploring 'engaged citizen' or socioscientific themes given their interwoven economic, political, scientific, and social dimensions. This article describes how an undergraduate course on the history of infectious diseases was modified to explore the impact of two 'engaged citizen' themes (poverty and technology), and to consider the ramifications of those themes on past, present, and future infectious disease outbreaks. Four outbreaks were used as the foundation for the course: plague (1350s), puerperal fever (1840s), cholera (1850s), and syphilis (1930s)...
October 14, 2016: FEMS Microbiology Letters
Yu Yang, Wendy Ballent, Brooke K Mayer
Phosphorus (P) is a critical, nonrenewable nutrient; yet excess discharges can lead to eutrophication and deterioration of water quality. Thus, P removal from water must be coupled with P recovery to achieve sustainable P management. P-specific proteins provide a novel, promising approach to recover P from water. Bacterial phosphate binding proteins (PBP) are able to effectively remove phosphate, achieving extremely low levels in water (i.e., 0.015 mg-P L(-1)). A prerequisite of using PBP for P recovery, however, is not only removal, but also controlled P release, which has not yet been reported...
October 14, 2016: FEMS Microbiology Letters
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