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FEMS Microbiology Letters

James I Prosser
No abstract text is available yet for this article.
October 2, 2017: FEMS Microbiology Letters
Bryan M Dewsbury
Faculty development of inclusive teaching practices has become more common in response to significant differences in STEM student retention between underrepresented minorities in the USA and students from other ethnic groups. Approaches to solve this have shifted from focusing on student deficits to changing campus culture, including the mindsets of instructors who teach STEM courses. In this article, I argue that based on the literature informing the conceptual frameworks used for faculty development in inclusive teaching, faculty developers should reframe the message of their workshops to focus participants more on the scope of the journey, and shift the direction of overall efforts some to redevelop pedagogical training at the graduate and postdoc levels...
October 2, 2017: FEMS Microbiology Letters
James P McEvoy
Active learning, including the promotion of student interactivity in lectures, has been found to improve student engagement and performance in university science classes. This letter describes the use of Pearson's Learning Catalytics to run regular, formatively assessed problem-solving sessions as part of the semiflipped redesign of an introductory level university bioscience course. Students found the problem-solving sessions more engaging than a traditional lecture, and felt that they were receiving better feedback on their progress in the course...
October 2, 2017: FEMS Microbiology Letters
Anna Petrovicheva, Jessica Joyner, Theodore R Muth
Agrobacterium tumefaciens is the causal agent of crown gall disease and is a vector for DNA transfer in transgenic plants. The transformation process by A. tumefaciens has been widely studied, but the attachment stage has not been well characterized. Most measurements of attachment have used microscopy and colony counting, both of which are labor and time intensive. To reduce the time and effort required to analyze bacteria attaching to plant tissues, we developed a quantitative real-time PCR (qPCR) assay to quantify attached A...
October 2, 2017: FEMS Microbiology Letters
Olivier Gros
Here, the first description is reported of an epsilon sulfur-oxidizing bacterium from sulfide-rich sediments of marine mangrove in the Caribbean. By transition electron microscopy it was shown that this new strain contains intracytoplasmic large internal sulfur granules, which was confirmed by energy-dispersive X-ray spectroscopy analyses performed using an environmental scanning electron microscope. The sulfur distribution obtained for this sulfur-oxidizing bacterial strain allowed us to conclude that elemental sulfur is formed as an intermediate oxidation product and stored intracellularly...
October 2, 2017: FEMS Microbiology Letters
J Jeukens, I Kukavica-Ibrulj, J G Emond-Rheault, L Freschi, R C Levesque
Antimicrobial resistance (AMR) is now recognized as a global threat to human health. The accessibility of microbial whole-genome sequencing offers an invaluable opportunity for resistance surveillance via the resistome, i.e. the genes and mutations underlying AMR. Unfortunately, AMR prediction from genomic data remains extremely challenging, especially for species with a large pan-genome. One such organism, for which multidrug-resistant (MDR) isolates are frequently encountered in the clinic, is Pseudomonas aeruginosa...
October 2, 2017: FEMS Microbiology Letters
Carla Andrea Alonso, Yakubu A Kwabugge, Madubuike U Anyanwu, Carmen Torres, Kennedy F Chah
Twenty-six lactose non-fermenting, oxidase, urease and citrate-positive Gram-negative rods, isolated from broiler chickens, pigs and cattle at slaughter, were subjected to the matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and 16S rDNA sequencing for identification. Susceptibility to 14 antimicrobials was determined by the disc diffusion method. Ochrobactrum isolates resistant to third-generation cephalosporins were PCR-screened for the presence of the Ochrobactrum anthropi ampC gene (blaOCH)...
September 15, 2017: FEMS Microbiology Letters
Pattra Charnchai, Sirima Suvarnakuta Jantama, Kaemwich Jantama
In this study, Bifidobacterium animalis subsp. lactis BF052 was demonstrated the growth capability in soymilk and could be thus supplemented as a probiotic starter that employed soymilk as one of its food vehicles. The complete genome sequence of BF052 was therefore determined to understand the genetic basis of BF052 as a technological and functional probiotic starter. The whole genome sequence of BF052 consists of a circular genome of 1938 624 bp with a G+C content of 60.50%. This research highlights relevant genes involving in its adaptive responses to industrial and/or environmental stresses and utilization of α-galacto-oligosaccharides in BF052 strain compared with other representative bifidobacterial genomes...
September 15, 2017: FEMS Microbiology Letters
Fernanda Kerche Paes da Silva, Dieter W Brück, Wolfram M Brück
The use of insects as a source of protein is becoming an important factor for feeding an increasing population. After protein extraction for food use, the insect exoskeleton may offer the possibility for the production of added value products. Here, the aim was to isolate bacteria from the surface of farmed mealworms (Tenebrio molitor Linnaeus, 1758) for the production of chitinous material from insect exoskeletons using microbial fermentation. Isolates were screened for proteases and acid production that may aid deproteination and demineralisation of insects through fermentation to produce chitin...
September 15, 2017: FEMS Microbiology Letters
Yiran Xu, Yongfei Liu, Feiran Li, Guoqiang Cao, Ping Zheng, Jibin Sun, Jianping Wen, Dawei Zhang
Threonine (Thr), an essential amino acid for mammals, has expanded its application to industries with fast-growing market demand. One factor that limits the production of L-threonine by microbial fermentation is the lack of an effective export system. This study proposes a method based on isobaric tags for relative and absolute quantification (iTRAQ) to determine potential Thr excretion proteins. Proteomics analysis revealed that an ABC family transport protein named YecC was upregulated in response to Thr addition...
September 15, 2017: FEMS Microbiology Letters
Liqun Xia, Haiying Liang, Liang Xu, Jianlin Chen, Michaël Bekaert, Honglian Zhang, Yishan Lu
Fish nocardiosis is a chronic systemic granulomatous disease, and Nocardia seriolae is the main pathogen that causes it. The pathogenesis and virulence factors of N. seriolae are not fully understood. A phospholipase C (PLC), which is likely to be a secreted protein targeting host cell mitochondria, was found by a bioinformatics analysis of the whole genome sequence of N. seriolae. In order to determine the subcellular localization and study the preliminary function of PLC from N. seriolae (NsPLC), in this study gene cloning, secreted protein identification, subcellular localization in host cells and apoptosis detection of NsPLC were carried out...
September 15, 2017: FEMS Microbiology Letters
Fuminori Sakai, Griffin Sonaty, David Watson, Keith P Klugman, Jorge E Vidal
Identification of Streptococcus pneumoniae and its more than 90 serotypes is routinely conducted by culture and Quellung reactions. Quantitative polymerase chain reactions (qPCRs) have been developed for molecular detection, including a pan-pneumococcus lytA assay, and assays targeting 79 serotypes. Reactions require genomic DNA from every target to prepare standards, which can be time consuming. In this study, we have developed a synthetic DNA molecule as a surrogate for genomic DNA and present new single-plex qPCR reactions to increase molecular detection to 94 pneumococcal serotypes...
September 15, 2017: FEMS Microbiology Letters
Yuming Li, Zhuanchang Wu, Ke Liu, Pengfei Qi, Jinpeng Xu, Jianchao Wei, Beibei Li, Donghua Shao, Yuanyuan Shi, Yafeng Qiu, Zhiyong Ma
Porcine reproductive and respiratory syndrome virus (PRRSV) has an important impact on the pig industry. Doxycycline (Dox) is a second-generation tetracycline widely used for treating bacterial infections. We evaluated the antiviral effect of Dox against PRRSV infection in Marc-145 cells that were susceptible to PRRSV infection. Dox significantly reduced the PRRSV-induced cytopathic effect and effectively restrained PRRSV replication in a dose-dependent manner. The 50% effective concentration of Dox was approximately 0...
September 15, 2017: FEMS Microbiology Letters
Cory A Leonard, J Russell Hayman
Microsporidia are obligate intracellular, spore-forming, fungal-related pathogens that employ a unique organelle, the polar tube, to transfer infectious spore contents into host cells to initiate infection. Spore adherence to host cells may provide the proximity required for polar tube/host cell interaction during in vivo infection. In previous in vitro studies, host sulfated glycosaminoglycans (GAGs) or recombinant microsporidia endospore protein (EnP1) was implicated in the pathogen adherence and infection process; however, complete ablation of spore adherence and infection could not be achieved, suggesting that additional or alternative spore and host cell determinants of adherence and infection may exist...
September 15, 2017: FEMS Microbiology Letters
Maria L Bonatelli, Maria C Quecine, Mariana S Silva, Carlos A Labate
The industrial ethanolic fermentation process is operated in distilleries, either in fed-batch or continuous mode. A consequence of the large industrial ethanol production is bacterial contamination in the fermentation tanks, which is responsible for significant economic losses. To investigate this community, we accessed the profile of bacterial contaminant from two distilleries in Brazil, each operating a different fermentation mode, throughout sugarcane harvest of 2013-2014. Bacterial communities were accessed through Illumina culture-independent 16S rDNA gene sequencing, and qPCR was used to quantify total bacteria abundance...
September 15, 2017: FEMS Microbiology Letters
Robert Dolan
No abstract text is available yet for this article.
September 15, 2017: FEMS Microbiology Letters
Hidemasa Nakaminami, Teruyo Ito, Xiao Han, Ayumu Ito, Miki Matsuo, Yuki Uehara, Tadashi Baba, Keiichi Hiramatsu, Norihisa Noguchi
SasX is a known virulence factor of Staphylococcus aureus involved in colonisation and immune evasion of the bacterium. The sasX gene, which is located on the ϕSPβ prophage, is frequently found in the sequence type (ST) 239 S. aureus lineage, which is the predominant healthcare-associated clone in Asian countries. In Japan, ST239 clones have rarely been identified, and sasX-positive strains have not been reported to date. Here, we report the first identification of 18 sasX-positive methicillin-resistant S...
September 1, 2017: FEMS Microbiology Letters
Angela Zhang, Austin L Carroll, Shota Atsumi
Atmospheric CO2 levels have reached an alarming level due to industrialization and the burning of fossil fuels. In order to lower the level of atmospheric carbon, strategies to sequester excess carbon need to be implemented. The CO2-fixing mechanism in photosynthetic organisms enables integration of atmospheric CO2 into biomass. Additionally, through exogenous metabolic pathways in these photosynthetic organisms, fixed CO2 can be routed to produce various commodity chemicals that are currently produced from petroleum...
September 1, 2017: FEMS Microbiology Letters
Letícia B Pereira, Bruna R Z Palermo, Camila Carlos, Laura M M Ottoboni
Corals harbor a wide diversity of bacteria associated with their mucus. These bacteria can play an important role in nutrient cycling, degradation of xenobiotics and defense against pathogens by producing antimicrobial compounds. However, the diversity of the cultivable heterotrophic bacteria, especially in the Brazilian coral species, remains poorly understood. The present work compares the diversity of cultivable bacteria isolated from the mucus and surrounding environments of four coral species present along the Brazilian coast, and explores the antibacterial activity of these bacteria...
September 1, 2017: FEMS Microbiology Letters
Zhu Zeng, Fanglei Zuo, Rui Yu, Bo Zhang, Huiqin Ma, Shangwu Chen
A novel lactose-responsive promoter of the ATP-binding cassette (ABC) transporter gene Lba1680 of Lactobacillus acidophilus strain 05-172 isolated from a traditionally fermented dairy product koumiss was characterized. In L. acidophilus 05-172, expression of Lba1680 was induced by lactose, with lactose-induced transcription of Lba1680 being 6.1-fold higher than that induced by glucose. This is in contrast to L. acidophilus NCFM, a strain isolated from human feces, in which expression of Lba1680 and Lba1679 is induced by glucose...
September 1, 2017: FEMS Microbiology Letters
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