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FEMS Microbiology Letters

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https://www.readbyqxmd.com/read/29145601/identification-and-functional-analysis-of-potential-prophage-derived-recombinases-for-genome-editing-in-lactobacillus-casei
#1
Yongping Xin, Tingting Guo, Yingli Mu, Jian Kong
Numerous LAB bacteriophage genomes have been sequenced, while the functional genes are yet to be exploited. In this study, a λ Red-like recombinase operon LCABL_13040-50-60 was identified from a prophage PLE3 in Lactobacillus casei BL23 genome, and its recombination function was confirmed by replacement of a 167 bp galK fragment with chloramphenicol-resistant gene (cat) in the L. casei BL23 genome. Further functional analysis showed that LCABL_13040 and LCABL_13060 were analogs to the host nuclease inhibitor (Redγ) and 5'-3' exonuclease (Redα/RecE), respectively...
November 14, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29126221/identification-and-characterization-of-mcr-mediated-colistin-resistance-in-extraintestinal-escherichia-coli-from-poultry-and-livestock-in-china
#2
Afrah Kamal Yassin, Jilei Zhang, Jiawei Wang, Li Chen, Patrick Kelly, Patrick Butaye, Guangwu Lu, Jiansen Gong, Min Li, Lanjing Wei, Yaoyao Wang, Kezong Qi, Xiangan Han, Stuart Price, Terri Hathcock, Chengming Wang
Antimicrobial resistance to colistin has emerged worldwide threatening the efficacy of one of the last-resort antimicrobials used for the treatment of multidrug-resistant Enterobacteriaceae infection in humans. In this study, we investigated the presence of colistin resistance genes (mcr-1, mcr-2, mcr-3) in Escherichia coli strains isolated from poultry and livestock collected between 2004 and 2012 in China. Furthermore, we studied the maintenance and transfer of the mcr-1 gene in E. coli after serial passages...
November 8, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29126137/study-of-mesophilic-aeromonas-salmonicida-a527-strain-sheds-light-on-the-species-lifestyles-and-taxonomic-dilemma
#3
Antony T Vincent, François D Rouleau, Sylvain Moineau, Steve J Charette
The Gram-negative bacterium Aeromonas salmonicida contains five subspecies: salmonicida, smithia, achromogenes, masoucida and pectinolytica. Pectinolytica is a mesophilic subspecies with the ability to thrive at a wide range of temperatures, including 37°C, while the four other subspecies are psychrophilic, thus restricted to lower temperatures. The psychrophilic subspecies are known to infect a wide range of fishes. However, there is no evidence of pathogenicity for the mesophilic subspecies pectinolytica...
November 8, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29126108/laboratory-scale-photobiotechnology-current-trends-and-future-perspectives
#4
Holger Morschett, Varun Loomba, Gregor Huber, Wolfgang Wiechert, Eric von Lieres, Marco Oldiges
Phototrophic bioprocesses are a promising puzzle piece in future bio-economy concepts but mostly fail for economic reasons, yet. Besides other aspects, this is mainly attributed to the omnipresent issue of optimal light supply impeding scale-up and -down of phototrophic processes according to classic established concepts. This MiniReview examines two current trends in photobiotechnology, namely microscale cultivation and modeling and simulation. Micro photobioreactors are a valuable and promising trend with microfluidic chips and microtiter plates as predominant design concepts...
November 8, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29121260/an-arf-gap-promotes-endocytosis-and-hyphal-growth-of-ashbya-gossypii
#5
Therese Oscarsson, Andrea Walther, Klaus B Lengeler, Jürgen Wendland
The ADP-ribosylation factor (ARF) family of GTPases are highly conserved from yeast to human and regulate vesicle budding. Sec7 domain containing proteins stimulate the guanine nucleotide exchange on Arf proteins while ARF-GTPase activating proteins stimulate the hydrolysis of GTP. Since vesicle trafficking is important for hyphal growth we studied the A. gossypii homolog of S. cerevisiae ARF3 along with its putative GEF and GAP encoded by YEL1 and GTS1, respectively. Deletion of YEL1 had no discernible phenotype and deletion of ARF3 had only a minor defect in vacuolar fusion...
November 7, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29121239/overproduction-of-s-meliloti-argc-n-acetyl-gamma-glutamyl-phosphate-reductase-promotes-growth-delay-and-inefficient-nodules
#6
Carmen Vargas-Lagunas, Yolanda Mora, Rafael Díaz, Gabriel Martínez-Batallar, Lourdes Girard, Sergio Encarnación, Humberto Peralta, Jaime Mora
argC encodes N-acetyl-gamma-glutamyl phosphate reductase, the enzyme that catalyzes the high-energy consuming third step in the arginine synthesis pathway. A comparative analysis revealed two translation start sites in argC from Sinorhizobium meliloti. To determine whether both protein versions are synthesized in the organism and their functional role, we obtained genetic constructs with one (1S) or two (2S) start sites, with promoters of low (pspeB) or high (plac) transcriptional rate. The constructs were transferred to the S...
November 7, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29126315/packaging-of-mycobacterium-smegmatis-bacteria-into-fecal-pellets-by-the-ciliate-tetrahymena-pyriformis
#7
Alix M Denoncourt, Valérie E Paquet, Steve J Charette
Mycobacteria are widespread microorganisms that live in various environments, including man-made water systems where they cohabit with protozoa. Environmental mycobacterial species give rise to many opportunistic human infections and can infect phagocytic protozoa. Protozoa such as amoebae and ciliates feeding on bacteria can sometimes get rid of non-digestible or pathogenic material by packaging it into secreted fecal pellets. Usually, packaged bacteria are still viable and are protected against chemical and physical stresses...
November 6, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29126297/tellurate-enters-escherichia-coli-k-12-cells-via-the-sult-type-sulfate-transporter-cyspuwa
#8
Jennifer Goff, Nathan Yee
Soluble forms of tellurium are environmental contaminants that are toxic to microorganisms. While tellurite [Te(IV)] is a well-characterized antimicrobial agent, little is known about the interactions of tellurate [Te(VI)] with bacterial cells. In this study, we investigated the role of sulfate transporters in the uptake of tellurate in Escherichia coli K-12. Mutant strains carrying a deletion of the cysW gene in the CysPUWA sulfate transporter system accumulated less cellular tellurium and exhibited higher resistance to tellurate compared to the wild type strain...
November 6, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29112725/peptide-transporter-yjiy-influences-the-expression-of-the-virulence-gene-mgtc-to-regulate-biofilm-formation-in-salmonella
#9
Preeti Garai, Kasturi Chandra, Jayanta Chatterjee, Dipshikha Chakravortty
Biofilm is one of the coping strategies of Salmonella against antimicrobial environmental stresses including nutrient starvation. However, channeling of the cue of starvation towards biofilm formation is not understood well. Our study shows that a carbon starvation gene, yjiY, coding for a peptide transporter, influences the expression of a virulence associated gene, mgtC, in Salmonella, to regulate biofilm formation. We demonstrate here that the mutant strain, ΔyjiY, is unable to form biofilm due to the increased expression of mgtC...
November 3, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29106534/effective-presentation-skills
#10
Robert Dolan
No abstract text is available yet for this article.
November 2, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29106533/ywhae-is-a-novel-interaction-partner-of-helicobacter-pylori-caga
#11
Xiaoyan Zhang, Bangwei Zeng, Chunyan Wen, Shurong Zheng, Hao Chen, Feifei She
CagA, an important virulence factor of Helicobacter pylori, targets and interacts with a series of host proteins to activate signaling factors involved in many functions, such as development, cytoskeleton rearrangement, and inflammatory molecule release. Despite extensive efforts, the relationship between CagA and gastric cancer is far from completely understood. Here, the GAL4 yeast two-hybrid system was used to screen cellular proteins for binding to CagA, and five cellular proteins, including tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE), were identified...
November 2, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29099921/methicillin-resistant-staphylococcus-aureus-in-minas-frescal-cheese-evaluation-of-classic-enterotoxin-genes-antimicrobial-resistance-and-clonal-diversity
#12
Alice Gonçalves Martins Gonzalez, Leila Márcia Peres Marques, Marcel da Silva Amorim Gomes, JhonathanCampos do Couto Beltrão, Marcos Gabriel Pinheiro, Luciana Maria Ramires Esper, Geraldo Renato de Paula, Lenise Arneiro Teixeira, Fábio Aguiar-Alves
This study aimed to investigate classical enterotoxin (sea to see) and mecA genes, by polymerase chain reaction (PCR) and anitimicrobial susceptibility, by disc diffusion test of Staphylococcus aureus isolated from minas frescal cheese (MFC). All methicillin resistant S. aureus (MRSA) isolates were investigated for the presence of PVL genes and clonal diversity. Thirty-one S. aureus were isolated from four MFC samples. Seven (22.6%) S. aureus carried mecA gene and two of them carried enterotoxin genes seb/sec and sea/seb...
November 1, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29095985/development-of-an-asporogenic-bacillus-cereus-strain-to-improve-keratinase-production-in-exponential-phase-by-switching-sigmah-on-and-sigmaf-off
#13
Fathollah Ahmadpour, Bagher Yakhchali
Many bacteria, including the genus Bacillus are able to produce proteases (keratinase). In Bacillus, proteases are produced in the stationary phase and initial stages of sporulation. Protease production is coordinated with sporulation in which expression of various genes by different sigma factors manages the transition from exponential to the stationary phase. In the present study the sigma-F gene of an indigenous Bacillus cereus strain, which is involved in transcription of maintaining sporulation genes was deleted...
October 31, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29092037/antibiotic-stewardship-a-european-perspective
#14
Céline Pulcini
Antibiotic stewardship programmes, which are a set of interventions aiming at prescribing antibiotics appropriately and responsibly, are one of the key strategies to tackle the current worldwide bacterial resistance crisis. We will present here some examples of international and national initiatives, with a focus on Europe, discuss the role of clinical microbiologists, and present some perspectives and future developments.Antibiotic stewardship (ABS) is one of the key strategies to tackle the current worldwide bacterial resistance crisis (https://ec...
October 30, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29092031/synechococcus-plasticity-under-environmental-changes
#15
Cristiana Callieri
Cyanobacteria are among the oldest photoautotrophic organisms on Earth, and have contributed to shaping the planet's biogeochemistry with their significant biomass and key metabolic activities. Synechococcus, the focus of this review, is one of the prevalent genera in the order Chroococcales, common in oceans and lakes and characterized by a coccoid unicellular or microcolony morphology. The evolution of its phycobilisomes is the key of the adaptation of this tiny photosynthetic cell to different light regimes and environmental conditions...
October 30, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29088370/antibiotic-treatment-disrupts-bacterial-communities-in-the-colon-and-rectum-of-siv-infected-macaques
#16
Alexander S Zevin, Tiffany Hensley-McBain, Charlene Miller, Elise Smith, Stanley Langevin, Nichole R Klatt
Antibiotic therapies are known to disrupt gastrointestinal (GI) bacterial communities. HIV and pathogenic simian immunodeficiency virus (SIV) infections have also been associated with disrupted GI bacterial communities. We administered a combination antibiotic therapy to six SIV-infected rhesus macaques (RM) and collected colon biopsies, stool samples, and rectal swabs before and after antibiotics, and evaluated the bacterial communities at each sample site using high-throughput 16S rRNA gene sequencing. The colon mucosa and stool samples displayed different bacterial communities, while the rectal swabs showed a mixture of the mucosal and stool-associated bacteria...
October 27, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29087459/regulatory-role-of-xynr-yagi-in-catabolism-of-xylonate-in-escherichia-coli-k-12
#17
Tomohiro Shimada, Eri Momiyama, Yuki Yamanaka, Hiroki Watanabe, Kaneyoshi Yamamoto, Akira Ishihama
The genome of Escherichia coli K-12 contains ten cryptic phages, altogether constituting about 3.6% of the genome in sequence. Among more than 200 predicted genes in these cryptic phages, 14 putative transcription factor (TF) genes exist, but their regulatory functions remain unidentified. As an initial attempt to make a breakthrough for understanding the regulatory roles of cryptic phage-encoded TFs, we tried to identify the regulatory function of CP4-6 cryptic prophage-encoded YagI with unknown function. After SELEX screening, YagI was found to bind mainly at a single site within the spacer of bidirectional transcription units, yagA (encoding another uncharacterized TF) and yagEF (encoding 2-keto-3-deoxy gluconate aldolase, and dehydratase, respectively) within this prophage region...
October 26, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29087456/yeast-phospholipase-c-is-required-for-stability-of-casein-kinase-i-yck2p-and-expression-of-hexose-transporters
#18
Tiantian Zhang, Luciano Galdieri, Jiri Hasek, Ales Vancura
Phospholipase C (Plc1p) in Saccharomyces cerevisiae is required for normal degradation of repressor Mth1p and expression of the HXT genes encoding cell membrane transporters of glucose. Plc1p is also required for normal localization of glucose transporters to the cell membrane. Consequently, plc1Δ cells display histone hypoacetylation and transcriptional defects due to reduced uptake and metabolism of glucose to acetyl-CoA, a substrate for histone acetyltransferases. In the presence of glucose, Mth1p is phosphorylated by casein kinase I Yck1/2p, ubiquitinated by the SCFGrr1 complex, and degraded by the proteasome...
October 26, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29069461/xdhr-negatively-regulates-actinorhodin-biosynthesis-in-streptomyces-coelicolor-m145
#19
Jiafang Fu, Gongli Zong, Peipei Zhang, Junxia Ma, Xiuhua Pang, Guangxiang Cao
The xdhR gene encodes a TetR-family regulator in Streptomyces coelicolor. However, little is known about the function of XdhR in regulating actinorhodin production. Here, we report that XdhR negatively regulates actinorhodin biosynthesis in S. coelicolor. Deletion of xdhR resulted in overproduction of actinorhodin by approximately 2.5-fold compared to the wild-type strain. Complementation of the xdhR deletion strain restored actinorhodin production to normal levels. In addition, the relative expression levels of actinorhodin cluster genes were all significantly increased in the xdhR deletion strain compared to the wild-type strain...
October 24, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29069450/improvement-of-a-yeast-self-excising-integrative-vector-by-prevention-of-expression-leakage-of-the-intronated-cre-recombinase-gene-during-plasmid-maintenance-in-escherichia-coli
#20
Michael O Agaphonov
The use of plasmids possessing a regulatable gene coding for a site-specific recombinase together with its recognition sequences significantly facilitates genome manipulations since it allows self-excision of the portion of the genetic construct integrated into the host genome. Stable maintenance of such plasmids in Escherichia coli , which is used for plasmid preparation, requires prevention of recombinase synthesis in this host, which can be achieved by interrupting the recombinase gene with an intron. Based on this approach, Saccharomyces cerevisiae and Hansenula polymorpha self-excising vectors possessing intronated gene for Cre recombinase and its recognition sites ( LoxP ) were previously constructed...
October 24, 2017: FEMS Microbiology Letters
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