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FEMS Microbiology Letters

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https://www.readbyqxmd.com/read/29351606/analysis-of-metal-tolerance-in-rhizobium-leguminosarum-strains-isolated-from-an-ultramafic-soil
#1
Laura Rubio-Sanz, Belén Brito, Jose Palacios
Natural habitats containing high amounts of heavy metals provide a valuable source of bacteria adapted to deal with metal toxicity. A functional analysis of the population of legume endosymbiotic bacteria in an ultramafic soil was undertaken by studying a collection of Rhizobium leguminosarum bv viciae (Rlv) isolates obtained using pea as trap plant. One of the isolates, Rlv UPM1137, was selected on the basis of its higher tolerance to nickel and cobalt and presence of inducible mechanisms for such tolerance...
January 17, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29351603/the-unexpected-function-of-a-flavin-dependent-oxidoreductase-fox-from-variovorax-paradoxus-tbea6
#2
Christina Meinert, Marc Schürmann, Jan-Eike Domeyer, Anja Poehlein, Rolf Daniel, Alexander Steinbüchel
3,3'-Thiodipropionic acid (TDP) is used as an additive in food and cosmetic industry and as precursor substrate for biotechnical polythioester production. Its catabolism was investigated in Variovorax paradoxus TBEA6 previous to this study. It was reported that the insertion of the transposon Tn5::mob into a gene showing high homology to flavin-dependent oxidoreductases (fox) resulted in impaired growth with TDP. Therefore, it was assumed that the initial cleavage of TDP is catalyzed by an FAD-dependent oxidoreductase (Fox, VPARA_05580)...
January 17, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29346641/uncovering-behavioural-diversity-amongst-high-strength-pseudomonas-spp-surfactants-at-the-limit-of-liquid-surface-tension-reduction
#3
Kamaluddeen Kabir, Yusuf Y Deeni, Simona M Hapca, Luke Moore, Andrew J Spiers
Bacterial bio-surfactants have a wide range of biological functions and biotechnological applications. Previous analyses had suggested a limit to their reduction of aqueous liquid surface tensions (γMin), and here we confirm this in an analysis of 25 Pseudomonas spp. strains isolated from soil which produce high-strength surfactants that reduce surface tensions to 25.2 ± 0.1-26.5 ± 0.2 mN.m-1 (the surface tension of sterile growth medium and pure water was 52.9 ± 0.4 mN.m-1 and 72.1 ± 1.2 mN.m-1, respectively)...
January 16, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29346648/esrb-negatively-regulates-expression-of-the-glutamine-sythetase-glna-in-the-fish-pathogen-edwardsiella-piscicida
#4
Yunpeng Guan, Kaiyu Yin, Mian Zhou, Minjun Yang, Yuanxing Zhang, Xiaohong Liu, Qiyao Wang
Edwardsiella piscicida is a gram-negative bacterial pathogen invading a wide range of fish species. Response regulator EsrB is essential for activation of type III and type VI secretion systems (T3/T6SS). In this study, proteomes of the wild type E. piscicida EIB202 and a ΔesrB mutant strains were compared to identify the regulon components of EsrB cultured in DMEM allowing T3/T6SS expression. As a result, 19 proteins showed different expression, which were identified to be associated with T3/T6SS, related to amino acid transport and metabolism, and energy production...
January 15, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29346538/vitamin-k5-is-an-efficient-photosensitizer-for-ultraviolet-a-light-inactivation-of-bacteria
#5
Fei Xu, Ying Li, Justen Ahmad, Yonggang Wang, Dorothy Scott, Jaroslav G Vostal
Photodynamic treatment combining light and a photosensitizer molecule can be an effective method to inactivate pathogenic bacteria. This study identified vitamin K5 as an efficient photosensitizer for ultraviolet light A (UVA)-induced bacterial inactivation. Six bacterial species, Bacillus cereus (vegetative form), Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, and two species of antibiotic-resistant bacteria, Pseudomonas aeruginosa* and Staphylococcus aureus*, were suspended in aqueous solutions with or without vitamin K5 and exposed to UVA irradiation...
January 15, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29325106/transcriptomic-profiling-of-interacting-nasal-staphylococci-species-reveals-global-changes-in-gene-and-non-coding-rna-expression
#6
Grith M M Hermansen, Pavelas Sazinas, Ditte Kofod, Andrew Millard, Paal Skytt Andersen, Lars Jelsbak
Interspecies interactions between bacterial pathogens and the commensal microbiota can influence disease outcome. In the nasal cavities, Staphylococcus epidermidis has been shown to be a determining factor for Staphylococcus aureus colonization and biofilm formation. However, the interaction between S. epidermidis and S. aureus has mainly been described by phenotypic analysis, and little is known about how this interaction modulates gene expression.This study aimed to determine the interactome of nasal S. aureus and S...
January 9, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29319821/probiotic-potency-of-lactobacillus-plantarum-kx519413-and-kx519414-isolated-from-honey-bee-gut
#7
C Honey Chandran, T R Keerthi
The Indian honey bee-Apis cerana indica, which harbors an abundant and diverse range of lactic acid bacteria (LAB) in their gut with beneficial effects was used as the source for the isolation of LAB. In the present study, two LAB isolates from honey bee gut were selected primarily based on their phenotypic and selective biochemical characterization, further PCR and identified using 16S rRNA sequencing as Lactobacillus plantarum and were registered in National Centre for Biotechnology Information (NCBI) under accession number KX519413 and KX519414...
January 8, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29319811/identification-of-a-consensus-motif-in-erg28p-required-for-c-4-demethylation-in-yeast-ergosterol-biosynthesis-based-on-mutation-analysis
#8
Xia Ke, Xiao-Yuan Xia, Ren-Chao Zheng, Yu-Guo Zheng
The Erg28p protein is localized to the endoplasmic reticulum (ER), where it acts as a scaffold to tether the C-4 demethylase complex involved in the sterol biosynthesis pathway of Saccharomyces cerevisiae. However, due to the challenges involved in characterizing the interactions of membrane proteins, the precise region of Erg28p that is responsible for the assembly of this enzyme complex remains unknown. To address this question, mutants with serial truncations in the C-terminus of Erg28p were constructed based on a topology prediction of its transmembrane domain...
January 8, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29319802/functional-analysis-of-arginine-decarboxylase-gene-spea-of-bacteroides-dorei-by-markerless-gene-deletion
#9
Mikiyasu Sakanaka, Yuta Sugiyama, Misaki Nara, Aya Kitakata, Shin Kurihara
Polyamine concentrations in the intestine are regulated by their biosynthesis by hundreds of gut microbial species and these polyamines are involved in host health and disease. However, polyamine biosynthesis has not been sufficiently analyzed in major members of the human gut microbiota, possibly owing to a lack of gene manipulation systems. In this study, we successfully performed markerless gene deletion in Bacteroides dorei, one of the major members of the human gut microbiota. The combination of a thymidine kinase gene (tdk) deletion mutant and a counter-selection marker tdk, which has been applied in other Bacteroides species, was used for the markerless gene deletion...
January 8, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29319784/the-effect-of-isabelin-a-sesquiterpene-lactone-from-ambrosiaartemisiifolia-on-soil-microorganisms-and-human-pathogens
#10
Francesco Molinaro, Olaf Tyc, Jules Beekwilder, Katarina Cankar, Cinzia Margherita Bertea, Michèle Negre, Paolina Garbeva
Ambrosia artemisiifolia L. (common ragweed) is an invasive weed, well-known for the strong allergenic effect of its pollen, as well as for its invasiveness and impact in crop fields (e.g. causing yield losses). This species produces a broad range of sesquiterpenoids. In recent years, new bioactive molecules have been discovered in this plant, e.g. isabelin, a sesquiterpene dilactone. The bioactivity of isabelin has been already demonstrated on allergy-related receptors and its inhibitory effect on seeds of various plant species...
January 8, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29315386/regulated-ploidy-of-bacillus-subtilis-and-three-new-isolates-of-bacillus-and-paenibacillus
#11
Benjamin Böttinger, Florian Semmler, Karolin Zerulla, Katharina Ludt, Jörg Soppa
Bacteria were long assumed to be monoploid, maintaining one copy of a circular chromosome. In recent years it became obvious that the majority of species in several phylogenetic groups of prokaryotes are oligoploid or polyploid. The present study aimed at investigating the ploidy inGram-positive aerobic endospore-forming bacteria. First, the numbers of origins and termini of the widely-used laboratory strain Bacillus subtilis 168 were quantified. The strain was found to be mero-oligoploid in exponential phase (5...
January 5, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29281013/characterization-of-a-novel-miia-domain-containing-protein-mdce-in-bradyrhizobium-spp
#12
David Durán, Juan Imperial, José Palacios, Tomás Ruiz-Argüeso, Michael Göttfert, Susanne Zehner, Luis Rey
Several genes coding for proteins with metal ion-inducible autocleavage (MIIA) domains were identified in type III secretion system tts gene clusters from draft genomes of recently isolated Bradyrhizobium spp. MIIA domains have been first described in the effectors NopE1 and NopE2 of Bradyrhizobium diazoefficiens USDA 110. All identified genes are preceded by tts box promoter motifs. The identified proteins contain one or two MIIA domains. A phylogenetic analysis of 35 MIIA domain sequences from 16 Bradyrhizobium strains revealed four groups...
December 21, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29272423/transcriptional-and-environmental-control-of-bacterial-denitrification-and-n2o-emissions
#13
Hannah Gaimster, Mark Alston, David Richardson, Andrew Gates, Gary Rowley
In oxygen-limited environments, denitrifying bacteria can switch from oxygen-dependent respiration to nitrate (NO3-) respiration in which the NO3- is sequentially reduced via nitrite (NO2-), nitric oxide (NO) and nitrous oxide (N2O) to dinitrogen (N2). However, atmospheric N2O continues to rise, a significant proportion of which is microbial in origin. This implies that the enzyme responsible for N2O reduction, nitrous oxide reductase (NosZ), does not always carry out the final step of denitrification either efficiently, or in synchrony with the rest of the pathway...
December 20, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29272380/vibrioferrin-production-by-the-food-spoilage-bacterium-pseudomonas-fragi
#14
Tamsyn Stanborough, Narelle Fegan, Shane M Powell, Mark Tamplin, P Scott Chandry
Pseudomonas fragi is a meat and milk spoilage bacterium with high iron requirements, however mechanisms of iron acquisition remain largely unknown. The aim of this work was to investigate siderophore production as an iron acquisition system for P. fragi. A vibrioferrin siderophore gene cluster was identified in 13 P. fragi and experiments were conducted with a representative strain of this group (F1801). Chromeazurol S assays showed P. fragi F1801 produced siderophores under iron starvation at optimum growth and refrigeration temperature...
December 20, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29267883/distinct-features-of-c-n-balance-regulation-in-prochlorococcus-sp-strain-mit9313
#15
María Agustina Domínguez-Martín, Antonio López-Lozano, Oriol Alberto Rangel-Zúñiga, Jesús Díez, José Manuel García-Fernández
The abundance and significant contribution to global primary production of the marine cyanobacterium Prochlorococcus have made it one of the main models in marine ecology. Several conditions known to cause strong effects on the regulation of N-related enzymes in other cyanobacteria lacked such effect in Prochlorococcus. Prochlorococcus sp. strain MIT9313 is one of the most early-branching strains among the members of this genus. In order to further understand the C/N control system in this cyanobacterium, we studied the effect of the absence of three key elements in the ocean, namely N, P and Fe, as well as the effect of inhibitors of the N assimilation or photosynthesis on the N metabolism of this strain...
December 18, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29253127/isolation-and-evaluation-of-cocktail-phages-for-the-control-of-multi-drug-resistant-escherichia-coli-serotype-o104-h4-and-e-coli-o157-h7-isolates-causing-diarrhea
#16
Doaa Safwat Mohamed, Eman Farouk Ahmed, Abobakr Mohamed Mahmoud, Rehab Mahmoud Abd El-Baky, James John
Escherichia coli serotype O157: H7 and E. coli O104: H4 are well known food borne pathogens causing sever enteric illness. Using bacteriophages as biocontrol agents of some food borne pathogens and multi-drug resistant bacteria has a great attention nowadays. This study aims to test the effect of cocktail phages on the growth of some food borne pathogens and MDR E. coli. Routine conventional PCR was used to confirm the identification of E. coli isolates. Double-layered culture technique was used to isolate phages from sewage water...
December 14, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29240953/influence-of-excess-branched-chain-amino-acid-uptake-by-streptococcus-mutans-in-human-host-cells
#17
Takafumi Arimoto, Rei Yambe, Hirobumi Morisaki, Haruka Umezawa, Hideo Kataoka, Shohei Matsui, Hirotaka Kuwata
Oral streptococci, including cariogenic bacterium Streptococcus mutans, comprise a large percentage of human supragingival plaque, which contacts both tooth surfaces and gingiva. Eukaryotic cells are able to take up macromolecules and particles, including bacteria, by endocytosis. Increasing evidence indicates endocytosis may be used as an entry process by bacteria. We hypothesized some endocytosed bacteria might survive and obtain nutrients, such as amino acids, until they are killed. To verify this hypothesis, we focused on bacterial utilization of branched-chain amino acids (BCAAs; isoleucine, leucine, and valine) in host cells...
December 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29240952/diversity-and-complexity-of-flavodiiron-no-o2-reductases
#18
Filipe Folgosa, Maria C Martins, Miguel Teixeira
Flavodiiron proteins (FDPs) are a family of enzymes endowed with nitric oxide (NO) or oxygen reductase activities, forming the innocuous nitrous oxide (N2O) or water molecules, respectively. FDPs are widespread in the three life kingdoms, and have a modular nature, being each monomer minimally constituted by a metallo-β-lactamase-like domain containing a catalytic diiron center, followed by a flavodoxin one, with a flavin mononucleotide (FMN). Since their discovery, additional domains have been found in FDPs, attached to the C-terminus, and containing either extra metal (iron) centers or extra flavin binding modules...
December 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29240942/overexpression-of-pdr16-confers-resistance-to-complex-sphingolipid-biosynthesis-inhibitor-aureobasidina-in-yeast-saccharomyces-cerevisiae
#19
Yuka Katsuki, Yutaro Yamaguchi, Motohiro Tani
Sphingolipids are essential for normal cell growth of yeast Saccharomyces cerevisiae. AureobasidinA (AbA), an antifungal drug, inhibits Aur1, an enzyme catalysing the synthesis of inositolphosphorylceramide, and induces a strong growth defect in yeast. In this study, we screened for multicopy suppressor genes that confer resistance to AbA, and identified PDR16. In addition, it was found that PDR17, a paralog of PDR16, also functions as a multicopy suppressor. Pdr16 and Pdr17 belong to a family of phosphatidylinositol transfer proteins; however, cells overexpressing the other members of the family hardly exhibited resistance to AbA...
December 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29240940/state-of-the-art-in-eukaryotic-nitrogenase-engineering
#20
Stefan Burén, Luis M Rubio
Improving the ability of plants and plant-associated organisms to fix and assimilate atmospheric nitrogen has inspired plant biotechnologists for decades, not only to alleviate negative effects on nature from increased use and availability of reactive nitrogen, but also because of apparent economic benefits and opportunities. The combination of recent advances in synthetic biology and increased knowledge about the biochemistry and biosynthesis of the nitrogenase enzyme has made the seemingly remote and for long unreachable dream more possible...
December 12, 2017: FEMS Microbiology Letters
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