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FEMS Microbiology Letters

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https://www.readbyqxmd.com/read/28333317/characterization-of-a-gene-encoding-a-membrane-protein-that-affects-exopolysaccharide-production-and-intracellular-mg2-concentrations-in-ensifer-meliloti
#1
Justin P Hawkins, Ivan J Oresnik
Exopolysaccharides play an important role in the physiology of a bacterial cell. Ensifer meliloti is capable of producing at least two types of exopolysaccharides (EPS); succinoglycan, and galactoglucan. In E. meliloti exopolysaccharides are best known for their role in mediating interaction with its symbiotic hosts. It was previously shown that high concentrations of Mg 2+ or K + were capable of suppressing the mucoid phenotype associated with galactoglucan production in an expR+ derivative of Rm1021. In an attempt to determine how Mg 2+ regulates galactoglucan production, SRmD363 was mutagenized and screened for mutants which were visibly mucoid at high concentrations of magnesium...
March 16, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333333/why-aren-t-women-choosing-stem-academic-jobs-observations-from-a-small-group-discussion-at-the-2016-american-society-for-microbiology-annual-meeting
#2
Elizabeth M Adamowicz
This commentary summarizes a small-group discussion that recently occurred at the American Society for Microbiology annual general meeting, ASM Microbe, in Boston, Massachusetts on June 16-20, 2016, on the topic 'why are so few women choosing to become academics?' Specifically, the discussion focused on asking what the actual and perceived barriers to academic STEM careers women face, and possible solutions to address them which would make women more likely to seek out academic careers. The conclusions reached suggest that, despite improvement in recent years, women and minorities still face complex barriers to STEM academic careers, and further research is needed to determine the best solutions to this problem...
March 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333314/characterization-of-e-type-colicinogenic-plasmids-from-shigella-sonnei
#3
Fatema Calcuttawala, Chellaram Hariharan, Gururaja P Pazhani, Dhira Rani Saha, Thandavarayan Ramamurthy
Colicinogenic plasmids encode toxic proteins which have antagonistic activity against closely related bacteria. This study describes the molecular characterization of three colicinogenic plasmids designated as pSSE3, pSSE and pSSE2, each with a molecular size of ∼6 kb, identified in clinical isolates of S. sonnei. Sequence analysis revealed that pSSE and pSSE2 shared extensive sequence homology with each other and with Escherichia coli E-type colicinogenic plasmids. The plasmid pSSE3 lacked an additional gene imparting immunity to colicin E8, a unique feature not observed in any of the previously reported sequences of colicin E3 plasmids...
March 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333308/fluconazole-treatment-enhances-extracellular-release-of-red-pigments-in-the-fungus-monascus-purpureus
#4
Sunil H Koli, Rahul K Suryawanshi, Chandrashekar D Patil, Satish V Patil
Traditional methods for the production of food grade pigments from fungus Monascus spp. are mostly relying on submerged fermentation. However, cell bound nature and intracellular accumulation of pigments in Monascus spp is the major hurdle in pigment production by submerged fermentation. The present study focused on the investigation of the effect of the antifungal agent, fluconazole on red pigment production from Monascus purpureus (NMCC-PF01). At the optimized concentration of fluconazole (30 μg/ml), pigment production was found to be enhanced by 88% after 96 h and it remained constant even after further incubation up to 168 h...
March 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333306/protein-aggregation-in-ehrlichia-chaffeensis-during-infection-of-mammalian-cells
#5
Dorota Kuczynska-Wisnik, Chuanmin Cheng, Roman R Ganta, Michal Zolkiewski
Ehrlichia chaffeensis is an obligatory intracellular pathogen transmitted through infected ticks to humans and other vertebrates. We investigated the extent of protein aggregation in E. chaffeensis during infection of canine macrophage cell line, DH82. We discovered that the size of the aggregated fraction of E. chaffeensis proteins increased during the first 48 h post infection. We also incubated the infected cells with guanidinium chloride (GuHCl), a known inhibitor of the protein-disaggregating molecular chaperone ClpB...
March 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333269/twin-layer-biosensor-for-real-time-monitoring-of-alkane-metabolism
#6
Tapio Lehtinen, Ville Santala, Suvi Santala
Intracellular metabolic sensors can be used for efficient screening and optimization of microbial cell factories. In particular, the sensors are useful in acquiring information about pathway dynamics and bottlenecks in a straightforward manner. Here, we developed a twin-layer biosensor which functions simultaneously at two levels: through transcription factor mediated sensing and enzyme-metabolite interaction, providing insights into the dynamics of alkane metabolism. In addition, the sensor can be used for monitoring either alkane degradation or biosynthesis, depending on the used cellular context...
March 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333255/alkaline-protease-contributes-to-pyocyanin-production-in-pseudomonas-aeruginosa
#7
Kazuhiro Iiyama, Eigo Takahashi, Jae Man Lee, Hiroaki Mon, Mai Morishita, Takahiro Kusakabe, Chisa Yasunaga-Aoki
The role of the alkaline protease (AprA) in pyocyanin production in Pseudomonas aeruginosa was investigated. AprA was overproduced when a plasmid carrying the aprA gene was introduced to an aprA-deletion mutant strain, EG03; thus, aprA-complemented EG03 was used as an overproducing strain. The complemented strain produced higher pyocyanin than the mutant strain in all commercially available media evaluated. Particularly, pyocyanin production was higher in the complemented than in the parental strain in brain-heart infusion and tryptic soy broths...
March 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333282/anchorless-cell-surface-proteins-function-as-laminin-binding-adhesins-in-lactobacillus-rhamnosus-fsmm22
#8
Ni Putu Desy Aryantini, Daisuke Kondoh, Keita Nishiyama, Yuji Yamamoto, Takao Mukai, I Nengah Sujaya, Tadasu Urashima, Kenji Fukuda
Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a two-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase, and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface...
March 9, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333278/quantifying-ribosome-dynamics-in-escherichia-coli-using-fluorescence
#9
Jurek Failmezger, Julian Ludwig, Alexander Nieß, Martin Siemann-Herzberg
Ribosomes are a crucial component of the physiological state of a cell. Therefore, we aimed to monitor ribosome dynamics using a fast and easy fluorescence readout. Using fluorescent-labeled ribosomal proteins, the dynamics of ribosomes during batch cultivation and during nutritional shift conditions were investigated. The fluorescence readout was compared to the cellular rRNA content determined by capillary gel electrophoresis with laser-induced fluorescence detection during exponentially accelerating and decelerating growth...
March 8, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333276/genetic-evidence-that-multiple-proteases-are-involved-in-modulation-of-heat-induced-activation-of-the-sigma-factor-sigi-in-bacillus-subtilis
#10
Tai-Yen Liu, Shu-Hung Chu, Yi-Nei Hu, Jyun-Jhih Wang, Gwo-Chyuan Shaw
The Bacillus subtilis sigI-rsgI operon encodes the heat-inducible sigma factor SigI and its cognate anti-sigma factor RsgI. The heat-activated SigI positively regulates expression of sigI itself and genes involved in cell wall homeostasis and heat resistance. It remains unknown which protease(s) may contribute to degradation of RsgI and heat-induced activation of SigI. In this study, we found that transcription of sigI from its σI-dependent promoter under heat stress was downregulated in a strain lacking the heat-inducible sigma factor SigB...
March 8, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333274/making-room-for-interactivity-using-the-cloud-based-audience-response-system-nearpod-to-enhance-engagement-in-lectures
#11
Stephen McClean, William Crowe
Active and collaborative learning provide distinct advantages for students in higher education, yet can often be hampered by the barrier of large class sizes. Solutions that combine a "bring your own device culture" (BOYD) with cloud-based technologies may facilitate a more interactive learning experience. In this pilot study we describe the use of one such technology, Nearpod, to enhance interactivity in lectures delivered to pharmacy and bioscience students at Ulster University. Existing material in PowerPoint or Keynote format is uploaded to the instructor area of Nearpod, interactive elements are added, and the lecture is then broadcast via the internet to student devices...
March 8, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333229/escherichia-coli-cattle-and-the-propagation-of-disease
#12
Richard A Stein, David E Katz
Several early models describing host-pathogen interaction have assumed that each individual host has approximately the same likelihood of becoming infected or of infecting others. More recently, a concept that has been increasingly emphasized in many studies is that for many infectious diseases, transmission is not homogeneous but highly skewed at the level of populations. In what became known as the "20/80 rule", about 20% of the hosts in a population were found to contribute to about 80% of the transmission potential...
March 1, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333237/new-paenibacillus-strain-produces-a-family-of-linear-and-cyclic-antimicrobial-lipopeptides-cyclization-is-not-essential-for-their-antimicrobial-activity
#13
En Huang, Xu Yang, Liwen Zhang, Sun Hee Moon, Ahmed E Yousef
A new bacterial isolate, Paenibacillus sp. OSY-N, showed potent antimicrobial activity against Gram-negative and Gram-positive bacteria. Antimicrobials produced by this strain were purified by reverse-phase high performance liquid chromatography (HPLC). Structural analysis, using mass spectrometry (MS), of a single active HPLC fraction revealed two known cyclic lipopeptides, BMY-28160 and permetin A, a new cyclic lipopeptide, and the linear counterparts of these cyclic compounds. The latter were designated as paenipeptin A, B and C, respectively...
February 25, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333234/horizontal-transfer-of-the-blandm-1-gene-to-pseudomonas-aeruginosa-and-acinetobacter-baumannii-in-biofilms
#14
Windy D Tanner, Robyn M Atkinson, Ramesh K Goel, Mark A Toleman, Lowell Scott Benson, Christina A Porucznik, James A VanDerslice
Horizontal gene transfer has contributed to the global spread of the blaNDM-1 gene. Multiple studies have demonstrated plasmid transfer of blaNDM-1 between Gram-negative bacteria, primarily Enterobacteriaceae species, but conjugational transfer of natural blaNDM-1 plasmids from Enterobacteriaceae into Pseudomonas aeruginosa and Acinetobacter baumannii has not previously been shown. As P. aeruginosa and A. baumannii are both typically strong biofilm-formers, transfer of natural blaNDM-1 plasmids could potentially occur more readily in this environment...
February 25, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333223/editorial-on-115-years-of-sulfur-microbiology
#15
Rich Boden
No abstract text is available yet for this article.
February 25, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333211/glutathione-affects-the-transport-activity-of-rhizobium-leguminosarum-3841-and-is-essential-for-efficient-nodulation
#16
Guojun Cheng, Ramakrishnan Karunakaran, Alison K East, Olaya Munoz-Azcarate, Philip S Poole
As glutathione (GSH) plays an essential role in growth and symbiotic capacity of rhizobia, a glutathione synthetase (gshB) mutant of Rhizobium leguminosarum biovar viciae 3841 (Rlv3841) was characterised. It fails to efficiently utilise various compounds as a sole carbon source, including glucose, succinate, glutamine and histidine, and shows 60-69% reduction in uptake rates of glucose, succinate and the non-metabolisable substrate α-amino isobutyric acid (AIB). The defect in glucose uptake can be overcome by addition of exogenous GSH, indicating GSH, but not its bacterial synthesis, is required for efficient transport...
February 23, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333204/proteins-ylaj-and-yhcn-contribute-to-the-efficiency-of-spore-germination-in-b-subtilis
#17
Christian L Johnson, Anne Moir
The YlaJ and YhcN spore lipoproteins of B. subtilis contain a common domain, and are of unknown function. Homologues of YlaJ or YhcN are widespread in Bacilli and are also encoded in those Clostridia that use cortex lytic enzymes SleB and CwlJ for cortex hydrolysis during germination. In B. subtilis, we report that single and double mutants lacking YlaJ and/or YhcN show a reduced rate of spore germination in L-alanine, with a delay in loss of heat resistance, release of DPA, and OD fall. If B. subtilis spores lack the cortex lytic enzyme CwlJ, spore cortex degradation and subsequent outgrowth to form colonies is strictly dependent on the other cortex lytic enzyme SleB, allowing a test of SleB function; in a cwlJ mutant background, the combined loss of both ylaJ and yhcN genes resulted in a spore population in which only 20% of spores germinated and outgrew to form colonies, suggesting that SleB activity is compromised...
February 23, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333199/effect-of-butyrate-and-lactobacillus-gg-on-a-butyrate-receptor-and-transporter-during-campylobacter-jejuni-exposure
#18
Gail A M Cresci, Paul C Mayer, Stuart A Thompson
Campylobacter jejuni (C. jejuni) frequently infects humans causing many gastrointestinal symptoms, fever, fatigue and several long-term debilitating diseases. Current treatment for campylobacteriosis includes rehydration and in some cases, antibiotic therapy. Probiotics are used to treat several gastrointestinal diseases. Butyrate is a short-chain fatty acid known to promote intestinal health. Interaction of butyrate with its respective receptor (HCAR2) and transporter (SLC5A8), both expressed in the intestine, is associated with water and electrolyte absorption as well as providing defense against colon cancer and inflammation...
February 23, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333198/a-tetracycline-inducible-integrative-expression-system-for-streptococcus-pneumoniae
#19
Marina Meiers, Anke Laux, Daniela Eichinger, Anne Sexauer, Patrick Marx, Ralph Bertram, Reinhold Brückner
Inducible gene expression systems are very useful to analyze cellular processes. The ability to switch the expression state of genes of interest may even be crucial if essential traits or genetic instability are involved. An integrative plasmid, pTEX2, was designed using the (anhydro)tetracycline-inducible promoter Pxyl/tet from staphylococcal plasmid pRAB11 to control gene expression in Streptococcus pneumoniae. The system was evaluated by expressing genes of the two-component regulatory system ciaRH of S...
February 21, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28204472/an-uncharacterized-gene-coding-a-conserved-lytic-transglycosylase-domain-rl4716-is-required-for-proper-cell-envelope-function-in-rhizobium-leguminosarum
#20
Kara D Neudorf, Christopher K Yost
No abstract text is available yet for this article.
February 15, 2017: FEMS Microbiology Letters
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