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FEMS Microbiology Letters

Akira Fukuda, Masaru Usui, Torahiko Okubo, Chie Tagaki, Nop Sukpanyatham, Yutaka Tamura
The spreading of antimicrobial-resistant Enterobacteriaceae, especially those co-harboring plasmid-mediated cephalosporin (bla) and colistin (mcr) resistance genes, is becoming increasingly problematic. As a vector, flies carry antimicrobial-resistant bacteria (ARB) into human and livestock habitats. To investigate ARB in flies, we collected 235 flies from 27 sites (18 urban areas, five pig farms, and four chicken farms) in Thailand during 2013-2015. Cefotaxime-resistant Enterobacteriaceae (CtxRE) and bla-positive CtxRE were isolated from 70 (29...
July 13, 2018: FEMS Microbiology Letters
Steven M Swift, Jerel J Waters, D Treva Rowley, Brian B Oakley, David M Donovan
Clostridium perfringens, a spore-forming anaerobic bacterium, causes food poisoning and gas gangrene in humans, and is an agent of necrotizing enteritis in poultry, swine and cattle. Endolysins are peptidoglycan hydrolases from bacteriophage that degrade the bacterial host cell wall causing lysis and thus harbor antimicrobial therapy potential. The genes for the PlyCP10 and PlyCP41 endolysins were found in prophage regions of the genomes from Clostridium perfringens strains Cp10 and Cp41, respectively. The gene for PlyCP10 encodes a protein of 351 amino acids, while the gene for PlyCP41 encodes a protein of 335 amino acids...
July 13, 2018: FEMS Microbiology Letters
Jia Yu, Xi Peng, Yuxi Wei, Yue Mi, Baojie Zhu, Ting Zhou, Zhen Yang, Yin Liu
The 16S-23S rDNA internal transcribed spacer (ITS) sequence, located in the rrn operon, has been analyzed and evaluated for use in phylogenetic analysis and the detection target of bacteria. The ITS region displays a high level of diversity, being present in multiple copies and displaying variability in both length and sequence, and it carries more phylogenetic information than 16S rDNA. However, appropriately identifying ITS regions to use in analyses is challenging. To solve this problem, we analyzed the ITS regions in Vibrio parahaemolyticus and predicted the secondary structure of each analogous rrn transcript...
July 13, 2018: FEMS Microbiology Letters
Helen Wang, Dominik Waluk, Ray Dixon, Stefan Nordlund, Agneta Norén
Metabolic regulation of Rhodospirillum rubrum nitrogenase is mediated at the post- translational level by the enzymes DraT and DraG when subjected to changes in nitrogen or energy status. DraT is activated during switch-off whileDraG is inactivated by reversible membrane association. We confirm here that the ammonium transporter, AmtB1, rather than its paralog AmtB2, is required for ammonium induced switch off. Amongst several substitutions at the N100 position in DraG, only N100K, failed to locate to the membrane following ammonium shock, suggesting loss of interaction through charge repulsion...
July 13, 2018: FEMS Microbiology Letters
J Redfern, L Bowater, M Crossle, J Verran
The increase in Antimicrobial resistance (AMR) microorganisms has been exacerbated by exposure to antimicrobial drugs (e.g. antibiotics). A solution to AMR may require academic researchers to not only contribute to the drug discovery pipeline through laboratory research, but also to engage and inform non-specialist audiences using a variety of interventions in order to change behaviour towards our use of antibiotics. In this paper, the authors describe a hands-on public engagement event focusing on AMR. 'A Spoonful of Soil', was created by drawing on the past experiences of the delivering team (also described), with planning focusing on clear concise messages, selection of an appropriate audience and ensuring the event would be of significant interest to the audience...
July 9, 2018: FEMS Microbiology Letters
Patricio Sánchez, Cristian Oliver, Mauricio Hernández, Marcos Cortés, Karla Valenzuela, Rafael A Garduño, Rubén Avendaño-Herrera, Alejandro J Yáñez
Piscirickettsia salmonis is an intracellular γ-proteobacteria and the etiological agent of piscirickettsiosis, which causes massive economic losses in the Chilean salmon industry. The type IV pili (T4P) play an important role in adherence to host cell surfaces and bacterial pathogenicity. T4P contains a variable number of components, as predicted in P. salmonis genomes. However, no studies have determined if P. salmonis possesses T4P. The aims of this investigation were to identify T4P components in the P...
July 6, 2018: FEMS Microbiology Letters
Mariko Tanno-Nakanishi, Yuichiro Kikuchi, Eitoyo Kokubu, Satoru Yamada, Kazuyuki Ishihara
Treponema denticola is a major pathogen in periodontal disease, and is frequently isolated from the lesions of patients with chronic periodontitis. T. denticola utilizes serum components as nutrient sources so as to colonize and proliferate in the gingival crevice. However, the mechanisms of serum utilization remain unclear. Therefore, the aim of the present study was to identify T. denticola serum utilization genes. Precultured T. denticola cells were suspended in a tryptone-yeast extract-gelatin-volatile fatty acids medium containing 0, 1%, and 10% serum, respectively, and incubated anaerobically for 17 h...
July 5, 2018: FEMS Microbiology Letters
Tania Selas Castiñeiras, Steven G Williams, Antony G Hitchcock, Daniel C Smith
Since the emergence of the biopharmaceutical industry in the 1980's, Escherichia coli, has played an important role in the industrial production of recombinant proteins and plasmid DNA for therapeutic use. Currently, advanced biopharmaceutical products, including rationally-designed recombinant proteins and viral-vector gene therapies, offer unprecedented promise for the long-term management, and even cure of disease. As such, E. coli remains an important production host for the biopharmaceutical industry. This review provides insight into the industrially-relevant strain engineering approaches used to enhance both the quantity and quality of these therapeutic products...
July 4, 2018: FEMS Microbiology Letters
Li-Na Xu, Shang Guo, Sheng-Wan Zhang
Three culinary-medicinal fungi and mushrooms (Agaricus bisporus AS2796, Helvella lacunosa X1 and Fomitiporia yanbeiensis S.Guo & L.Zhou) were individually inoculated into different cereal grains (wheat, rice, oat, corn, millet, quinoa, buckwheat, soybean, pea and sorghum) and the antioxidant properties of fungus-fermented products after solid-state fermentation (SSF) (0, 7, 14, 21, 28 and 35 days; 25°C) were studied.The results showed that the total phenol contents (TPCs) of the fermented cereals varied with fermentation time and the starter organisms...
July 4, 2018: FEMS Microbiology Letters
Na Chang, Shunyu Yao, Deguang Chen, Lei Zhang, Jinhai Huang, Lilin Zhang
Cadmium is a heavy metal that cause irreversible hazard to the living organisms. Cadmium ion can induces the phosphorylation of MAPKs pathway molecules, such as Hog1 and Slt2, but downstream effectors and potential activation pathway were still unclear. In this study, the RNA-seq data analysis in cadmium-stressed yeast was performed to predict and screen the signal transduction pathway and the potential effect molecules regulated by MAPKs. Based on DEGs and Venn diagram, 31 genes regulated by Hog1p and 2 genes induced by Slt2p, which related to carbohydrate metabolism, oxidative damage, DNA replication stress and detoxification were characterized under Cd exposure yeast...
July 4, 2018: FEMS Microbiology Letters
Eveline M van den Berg, Martijn van Veen, Catherine Cotton, Bauke Oudega
No abstract text is available yet for this article.
July 4, 2018: FEMS Microbiology Letters
Doria Naila Bouchedja, Sabine Danthine, Tambi Kar, Patrick Fickers, Hosni Sassi, Abdelghani Boudjellal, Christophe Blecker, Frank Delvigne
The oleaginous yeast Yarrowia lipolytica has the ability to use oils and fats as carbon source, making it a promising cell factory for the design of alternative bioprocesses based on renewable substrates. However, such a multiphasic bioreactor design is rather complex and leads to several constraints when considering emulsification of the oil-in-water mixture, foaming and cell growth/physiology on hydrophobic substrate. This study aims to shed light on the effect of pH changes on the physico-chemical properties of the cultivation medium and on cell physiology...
July 4, 2018: FEMS Microbiology Letters
Fernando Pérez-García, Volker F Wendisch
Corynebacterium glutamicum has a long and successful history in the biotechnological production of the amino acids l-glutamate and l-lysine. In the recent years, C. glutamicum has been engineered for the production of a broad catalog of value-added compounds including organic acids, vitamins, terpenoids and proteins. Moreover, this bacterium has been engineered to realize a flexible carbon source concept enabling product formation from various second generation feedstocks without competing uses in human and animal nutrition...
June 30, 2018: FEMS Microbiology Letters
Kaitlyn N Wells, Patrick Videau, Dylan Nelson, Jessie E Eiting, Benjamin Philmus
Cyanobacterial natural products offer new possibilities for drugs and lead compounds but many factors can inhibit the production of sufficient yields for pharmaceutical processes. While Escherichia coli and Streptomyces sp. have been used as heterologous expression hosts to produce cyanobacterial natural products, they have not met with resounding success largely due to their inability to recognize cyanobacterial promoter regions. Recent work has shown that the filamentous freshwater cyanobacterium Anabaena sp...
June 30, 2018: FEMS Microbiology Letters
Yong-Chun Liu, Ri-Ming Huang, Jie Bao, Ke-Yue Wu, Heng-Yu Wu, Xiang-Yang Gao, Xiao-Yong Zhang
The microbes associated with black corals remain poorly studied. The present study is the first attempt to investigate microbial community structure in the black corals Antipathes ceylonensis and A. dichotoma from the South China Sea by using high-throughput Illumina sequencing. A total of 52 bacterial and 3 archaeal phyla were recovered in this study, suggesting the black corals harboured highly diverse microbial communities. Among the 55 microbial phyla, Proteobacteria, Firmicutes, Bacteroidetes, Chloroflexi, Acidobacteria and Actinobacteria dominated in the two black corals from the South China Sea...
June 30, 2018: FEMS Microbiology Letters
María José Valderrama, Bruno González-Zorn, Pilar Calvo de Pablo, Rosalía Díez-Orejas, Teresa Fernández-Acero, Jessica Gil-Serna, Lucía de Juan, Humberto Martín, María Molina, Federico Navarro-García, Belén Patiño, Jesús Pla, Daniel Prieto, Carmina Rodríguez, Elvira Román, Ana Belén Sanz-Santamaría, María Isabel de Silóniz, Mónica Suárez, Covadonga Vázquez, Víctor J Cid
The Small World Initiative (SWI) is a consolidated and successful education program rooted in the USA that tackles the antibiotic crisis by a crowdsourcing strategy. Based on active learning, it challenges young students to discover novel bioactive-producing microorganisms form environmental soil samples. Besides its pedagogical efficiency to impart Microbiology contents in academic curricula, SWI promotes vocations on research and development in Experimental Sciences and, at the same time, disseminates the antibiotic awareness guidelines of the World Health Organization (WHO)...
June 30, 2018: FEMS Microbiology Letters
Aniket Saraf, Himanshu G Dawda, Archana Suradkar, Priyanka Batule, Isha Behere, Manasi Kotulkar, Ankita Kumat, Prashant Singh
A false branching cyanobacterium (strain 10A1_PS) was isolated from a freshwater body of the Pachmarhi Biosphere Reserve, India and was characterized using the polyphasic approach. The detailed morphological examination indicated that the strain belonged to the complex genus Scytonema as it exhibited typical false branching character whose frequency increased with age of the culture. As the family Scytonemataceae and the genus Scytonema has been shown to be polyphyletic in many studies, we provide deep insights into the phylogenetic complexities within the family Scytonemataceae based on 16S rRNA gene phylogeny along with complete morphological, molecular and phylogenetic characterization of the strain...
June 30, 2018: FEMS Microbiology Letters
Xiaoqiang Jia, Tingting Zhao, Yilin Liu, Rongrong Bu, Kang Wu
To improve the performance of a whole-cell biosensor for lead detection, we designed six gene circuits by re-configuring the regulatory elements and incorporating positive feedback loops to the circuits. The lead resistance operon pbr encodes six genes with pbrRT on one side of the promoter and pbrABCD on the other side. PbrR, the divergent promoter it regulates, and GFP were used to design the lead biosensors. One has pbrR and gfp on opposite sides of the promoter mimicking the native operon. We re-configured it by placing pbrR and gfp on the same side or under two separate promoters...
June 28, 2018: FEMS Microbiology Letters
Jingru Zhao, Shouli Yuan, Bin Gao, Shunyi Zhu
Peptides with an inhibitor cystine knot (ICK) motif are extensively present in animals and plants where they exert a diversity of biological functions. However, few studies have been undertaken on this class of peptides in fungi. In this work, we identify a total of 386 fungal ICK peptides and proteins containing this motif by computational data mining of fungal genome databases, which exhibit 14 different exon-intron structures. According to their domain architectures, these proteins are classified into three distinct structural types, including single domains, tandem repeat domains and fusion domains, in which six families belonging to single and tandem repeat domains show remarkable sequence similarity to those from animals and plants, suggesting their orthologous relationship...
June 28, 2018: FEMS Microbiology Letters
Beatriz Casasola-Rodríguez, Guillermo M Ruiz-Palacios, Ramos-Cervantes Pilar, Luis Losano, Monje-Ramírez Ignacio, María Teresa Orta de Velásquez
The recognition of the viable but non-culturable (VBNC) state of pathogenic bacteria has brought with it many questions to answer related to the need to detect and quantify viable bacteria in the environment in an accurate way. To assess viability of Vibrio cholerae, we developed a RT-Real Time PCR technique based on differential expression analysis from mRNA deep sequencing data. We compared two induction conditions to achieve the VBNC state: a bacterial suspension induced by artificial seawater at 4 °C, and the addition of c-di-GMP...
June 28, 2018: FEMS Microbiology Letters
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