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Veterinary Microbiology

Miranda Prats-van der Ham, Juan Tatay-Dualde, Christian de la Fe, Ana Paterna, Antonio Sánchez, Juan Carlos Corrales, Antonio Contreras, Ángel Gómez-Martín
No abstract text is available yet for this article.
December 27, 2017: Veterinary Microbiology
Abbey J Steckler, Maria X Cardenas-Alvarez, Megan K Townsend Ramsett, Neil Dyer, Teresa M Bergholz
Listeria monocytogenes infections are an important disease of ruminants worldwide, causing encephalitis, septicemia, and abortions. Ruminant listeriosis can also pose a food safety risk due to the potential for L. monocytogenes to enter the food supply via the farm environment. Data on the genetic diversity of L. monocytogenes from ruminant clinical cases in the United States is limited. Our goal was to assess the genetic diversity of clinical listeriosis isolates from ruminants in the Upper Great Plains states, a population not well-studied, and compare this population to isolates from ruminants in New York State...
December 26, 2017: Veterinary Microbiology
Constantinos S Kyriakis, Ming Zhang, Stefan Wolf, Les P Jones, Byoung-Shik Shim, Anna H Chocallo, Jarod M Hanson, MingRui Jia, Dong Liu, Ralph A Tripp
No abstract text is available yet for this article.
December 20, 2017: Veterinary Microbiology
Silvia De Carli, Tiago Gräf, Diéssy Kipper, Fernanda Kieling Moreira Lehmann, Nathalie Zanetti, Franciele Maboni Siqueira, Samuel Cibulski, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge
No abstract text is available yet for this article.
December 7, 2017: Veterinary Microbiology
Sara Muñoz-González, Yusmel Sordo, Marta Pérez-Simó, Marisela Suarez, Albert Canturri, Maria Pilar Rodriguez, María Teresa Frías-Lepoureau, Mariano Domingo, Mario Pablo Estrada, Llilianne Ganges
Here we evaluated the effect of double vaccination with a novel subunit marker vaccine candidate based in the CSFV E2 glycoprotein fused to the porcine CD154 to prevent CSFV vertical transmission. A lentivirus-based gene delivery system was used to obtain a stable recombinant HEK 293 cell line for the expression of E2 fused to porcine CD154 molecule. Six pregnant sows were distributed in two groups and at 64days of gestation animals numbered 1-4 (group 1) were vaccinated via intramuscular inoculation with 50μg of E2-CD154 subunit vaccine...
November 7, 2017: Veterinary Microbiology
Luciana C C Lacerda, Andressa de Souza-Pollo, Ivan Ricardo M Padua, Luciano F Conceição, Camila P Balthazar da Silveira, Germana A Silva, Renato P Maluta, José L Laus
Bacterial contamination of the anterior chamber during cataract surgery is one of the main responsible for endophthalmitis postoperative. Phacoemulsification is a less invasive technique for cataract treatment, although it does not exclude the possibility of contamination. In this study, bacterial contaminants of aqueous humor collected pre- and post-phacoemulsification with intraocular lens implantation (IOL) of twenty dogs were identified. As the conjunctival microbiota constitute a significant source of anterior chamber contamination, bacterial isolates from aqueous humor were genetically compared with those present in the conjunctival surface of the patients...
January 2018: Veterinary Microbiology
Ting Huang, Xuhao Song, Kelei Zhao, Jie Jing, Yongmei Shen, Xiuyue Zhang, Bisong Yue
Trueperella pyogenes is a gram-positive opportunistic pathogen normally causes mastitis, liver abscesses and pneumonia of economically important livestock. It has been suggested that gram-negative bacteria can suppress the growth and virulence of T. pyogenes in vitro by using the quorum-sensing (QS) signal molecules and cause the transition of predominant species. However, whether these QS signals can be used as potential anti-virulence drugs against T. pyogenes infection is unclear. In this study, the in vivo inhibitory effect N-acyl homoserine lactones (AHLs) from Escherichia coli and Pseudomonas aeruginosa on T...
January 2018: Veterinary Microbiology
Pauline L C Zhang, Xiao Shen, Gabhan Chalmers, Richard J Reid-Smith, Durda Slavic, Hani Dick, Patrick Boerlin
There is little information on the genetic basis of resistance to the critically important extended-spectrum cephalosporins (ESCs) in Enterobacteriaceae from dogs in Canada. This study assessed the frequency of ESC resistance in Enterobacteriaceae isolated from dogs in Ontario and the distribution of major ESC resistance genes in these bacteria. A total of 542 Enterobacteriaceae were isolated from 506 clinical samples from two diagnostic laboratories in Ontario. Eighty-eight ESC-resistant Enterobacteriaceae and 217 Escherichia coli were isolated from 234 fecal samples from dogs collected at leash-free dog parks...
January 2018: Veterinary Microbiology
Anno de Jong, Farid El Garch, Shabbir Simjee, Hilde Moyaert, Markus Rose, Myriam Youala, Ed Siegwart
VetPath is an ongoing pan-European antimicrobial susceptibility monitoring programme collecting pathogens from diseased cattle, pigs and poultry not recently treated with antibiotics. Non-duplicate milk samples were collected from cows with acute clinical mastitis in nine countries and 934 isolates were obtained during 2009-2012 for subsequent antimicrobial susceptibility testing in a central laboratory. CLSI broth microdilution methodology was used, and where available, MICs were interpreted using CLSI approved veterinary-specific (ceftiofur) otherwise human clinical breakpoints...
January 2018: Veterinary Microbiology
Francesca Romana Massacci, Chiara Francesca Magistrali, Lucilla Cucco, Ludovica Curcio, Luca Bano, PierMario Mangili, Eleonora Scoccia, Magne Bisgaard, Bent Aalbæk, Henrik Christensen
In rabbit, P. multocida is considered a predominant pathogenic agent; despite this, few data on the molecular epidemiology are available so far. The aim of this work was to characterize P. multocida isolates from rabbit affected by various diseases in Italy. Comparison was made to reference strains from other countries. Thirty-nine isolates were tested using PCRs to detect the genes coding capsular antigens, virulence factors and lipopolysaccharide structures (LPS). Multilocus sequence typing (MLST) was performed and 19 STs registered that belonged to 9 clonal complexes...
January 2018: Veterinary Microbiology
Kate A Worthing, Sam Abraham, Geoffrey W Coombs, Stanley Pang, Sugiyono Saputra, David Jordan, Darren J Trott, Jacqueline M Norris
Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is an increasingly prevalent pathogen in veterinary medicine. This study examined the molecular epidemiology of clinical MRSP isolated from Australian animals. Clinical staphylococci submitted to all Australian veterinary diagnostic laboratories were collected during 2013 and identified using traditional phenotypic tests and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Phenotypic antimicrobial resistance was determined using broth microdilution and disk diffusion...
January 2018: Veterinary Microbiology
Li-Li Ma, Zhen-Hong Sun, Yu-Lin Xu, Shu-Juan Wang, Hui-Ning Wang, Hao Zhang, Li-Ping Hu, Xiao-Mei Sun, Lin Zhu, Hong-Qi Shang, Rui-Liang Zhu, Kai Wei
H9N2 subtype low pathogenic avian influenza virus (LPAIV) is distributed worldwide and causes great economic losses in the poultry industry, especially when complicated with other bacterial infections. Tissue damages caused by virus infection provide an opportunity for bacteria invasion, but this mechanism is not sufficient for low pathogenic strains. Moreover, although H9N2 virus infection was demonstrated to promote bacterial infection in several studies, its mechanism remained unclear. In this study, infection experiments in vivo and in vitro demonstrated that the adhesion of Escherichia coli (E...
January 2018: Veterinary Microbiology
Kinga M Sulyok, Katinka Bekő, Zsuzsa Kreizinger, Enikő Wehmann, Ákos Jerzsele, Zsuzsanna Rónai, Ibolya Turcsányi, László Makrai, Levente Szeredi, Szilárd Jánosi, Sára Ágnes Nagy, Miklós Gyuranecz
Determining the antibiotic susceptibility profile of Mycoplasma bovis isolates in vitro provides the basis for the appropriate choice of antibiotics in the therapy. Traditionally, the antibiotic susceptibility examination of mycoplasmas is technically demanding, time-consuming and rarely performed in diagnostic laboratories. The aim of the present study was to develop rapid molecular assays to determine mutations responsible for elevated minimal inhibitory concentrations (MICs) to fluoroquinolones, tetracyclines, aminocyclitols, macrolides, lincosamides, phenicols and pleuromutilins in M...
January 2018: Veterinary Microbiology
Farid El Garch, Anno de Jong, Xavier Bertrand, Didier Hocquet, Marlène Sauget
We evaluate here the presence of the mcr-1-like and mcr-2 genes in Escherichia coli and Salmonella spp. isolated from healthy food-producing animals at slaughter between 2002 and 2014 in Europe. Isolates were retrieved from cattle, pig and chicken from 11 European countries of production. The susceptibility to colistin and antibiotics used in human medicine was determined by agar dilution. Colistin-resistant isolates were PCR-screened for mcr genes. mcr-positive isolates were typed by Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing...
January 2018: Veterinary Microbiology
Shuanghu Cai, Haiyan Cheng, Huanying Pang, Jichang Jian, Zaohe Wu
V. alginolyticus is an important opportunistic pathogen which causes vibriosis in aquatic animals. AcfA, as an accessory colonization factor, is hypothesized to be involved in the pathogenesis of infection. In this study, a mutant strain with an in-frame deletion removed nucleotides 86 to 561 of the acfA gene was constructed to reveal the role of AcfA in the physiology and virulence from V. alginolyticus. An acfA mutant showed a similar growth level, an obvious decrease in swarming motility and the activity of ECPase, a higher LD50 value by intraperitoneal injection of grouper fish compared to that of the wild-type...
January 2018: Veterinary Microbiology
Laura Del Río, Antonio Murcia, Antonio J Buendía, Daniel Álvarez, Nieves Ortega, José A Navarro, Jesús Salinas, María Rosa Caro
Chlamydia abortus, like other members of the family Chlamydiaceae, have a unique intracellular developmental cycle that is characterized by its chronic nature. Infection of a flock can remain undetected for months, until abortion occurs the following reproductive season but, to date, neither the location nor the mechanisms that maintain this latent phase are fully understood. Studies have shown that IL-10 produced as a response to certain micro-organisms sustains the intracellular survival of pathogens and increases host susceptibility to chlamydial infections...
January 2018: Veterinary Microbiology
Santiago Mirazo, Noemí R Gardinali, D'Albora Cecilia, Lorenzo Verger, Florencia Ottonelli, Natalia Ramos, Gustavo Castro, Marcelo A Pinto, Viviana Ré, Belén Pisano, Alejandra Lozano, Jaqueline Mendes de Oliveira, Juan Arbiza
Hepatitis E virus (HEV) infection is an issue of public health concern in high-income and non-endemic countries. Increasing evidence supports the hypothesis of a zoonotic route as the main mode of infection in this epidemiological setting, since the transmission of genotypes HEV-3 and HEV-4 from reservoirs to humans has been demonstrated. In America, studies have confirmed the circulation of HEV in pig herds but the zoonotic role of wild boars has never been evaluated. Uruguay has a high burden of HEV- associated acute hepatitis, and a close phylogenetic relationship was observed among human HEV-3 strains and European isolates detected in swine...
January 2018: Veterinary Microbiology
Qian Jiang, Zuo Yu, Jia-Sen Liu, De-Sheng Kong, Dong-Chun Guo, Chuan-Song Quan, Bo-Tao Li, Xiao-Liang Hu, Liandong Qu
Rabbit hemorrhagic disease virus (RHDV) is responsible for rabbit hemorrhagic disease (RHD), which is an acute, lethal and highly contagious disease in both wild and domestic rabbits. Although current vaccines are highly effective for controlling RHD, they are derived from infected rabbit livers and their use is thus associated with safety and animal-welfare concerns. In this study, we generated a recombinant lentogenic canine adenovirus type 2 (CAV2) vector expressing the RHDV vp60 gene, named rCAV2-VP60. rCAV2-VP60 expressed VP60 protein in Madin-Darby canine kidney cells as demonstrated by western blot and immunofluorescence assay...
January 2018: Veterinary Microbiology
Birgitta Duim, Koen M H W Verstappen, Ruwani S Kalupahana, Lakmali Ranathunga, Ad C Fluit, Jaap A Wagenaar
The presence and genetic characteristics of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in Sri Lanka was investigated to add additional insight into global spread, emergence and evolution of MRSP. A total of 234 samples from dogs visiting veterinary clinics were cultured for staphylococci and the genomes of the MRSP isolates were sequenced, to identify resistance genes, the multilocus sequence types (MLST) and spa types. From a questionnaire the history of antimicrobial treatment and patient information was obtained...
January 2018: Veterinary Microbiology
Jinlong Cheng, Caiyun Huo, Jing Zhao, Tong Liu, Xiao Li, Shihong Yan, Zhujun Wang, Yanxin Hu, Guozhong Zhang
Infectious bronchitis is a highly contagious, acute viral respiratory disease of chickens, caused by infectious bronchitis virus (IBV). In recent years, the isolation rate of QX-like IBV has increased in the world. To clarify this phenomenon and better understand the pathogenicity of QX-like IBV, we examined differences in pathogenicity between two IBV strains, SD and M41, which belong to QX-like and Mass-type IBV, respectively. SD strain was more virulent in 3-week-old specific-pathogen-free chickens than M41 strain causing higher mortality with severe renal lesions...
January 2018: Veterinary Microbiology
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