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Anatomia, Histologia, Embryologia

Ahmed M Abdellatif, Mohamed A Hamed, El-Sayed El-Shafaey, Haitham Eldoumani
Buffaloes represent a major source of milk production, especially in developing countries including Egypt. The buffalo foot is frequently involved in a large proportion of lameness cases. The relatively small size and complexity of its structures often render the radiographic evaluation of the foot challenging. Magnetic resonance imaging (MRI) is a noninvasive imaging technique that is regarded as both safe and accurate for assessment of the foot disorders in both man and animals. The purpose of the current investigation was to describe the MRI anatomy of buffalo foot using cadaveric hind feet...
September 16, 2018: Anatomia, Histologia, Embryologia
Andhika Yudha Prawira, Savitri Novelina, Huda Salahudin Darusman, Wartika Rosa Farida, Srihadi Agungpriyono
Skin becomes the largest organ in the body and protects its own inner layer. The structure and chemical composition of the skin contribute to skin condition and affect the habitat of certain bacteria. The Sunda Porcupine is one of endemic animals of Indonesia which possesses quill as the main derivate of its skin and as a defence tool against predators. The present study used nine adults (five females and four males) of Sunda Porcupine and aimed to observe the correlation of skin structure with bacterial population at the surface level...
September 11, 2018: Anatomia, Histologia, Embryologia
Bruno C Schimming, Maria D Cesario, Selma M M Matheus
Opossums, the largest marsupials found in Brazil, are animals of great interest due to their commonality in large urban centres and their frequent use as laboratory animals. Thus, this study was designed to describe the vaginal complex in ten female opossums by gross anatomy and light microscopy. The vaginal complex comprises two lateral vaginae, a vaginal cul-de-sac and a urogenital sinus. No marked morphological contrast of the vaginal complex was observed in the proestrus and metestrus phases of the females studied...
September 7, 2018: Anatomia, Histologia, Embryologia
Yanping Ding, Na Zhang, Jialong Li, Yiran Jin, Baoping Shao
Ghrelin is a very important brain-gut peptide that modulates appetite and energy metabolism in mammals. The yak is the only large mammal that can adapt to the cold temperatures and hypoxia conditions present in the Qinghai-Tibet Plateau. However, there are no reports on ghrelin molecular characterization and expression in the hypothalamus-pituitary-digestive tract axis of the yak to date. In this study, the coding region sequence of the yak ghrelin, containing a complete ORF (351) encoding for 117 amino acids, was cloned...
September 3, 2018: Anatomia, Histologia, Embryologia
Christian Roski, Christiane Langrock, Nicole Körber, Gunnar Habermann, Eberhard Buse, Andreas Reichenbach, Thomas Pannicke, Mike Francke
Ca2+ -binding proteins are differentially expressed in the nervous system; their functional role often remains unclear. This immunohistochemical study aimed at characterising and comparing the expression pattern of the Ca2+ -binding proteins calbindin (Calb), calretinin (Calr) and parvalbumin (Parv) in the retina of four species of macaque monkeys: Macaca fascicularis (cynomolgus macaque), M. mulatta (rhesus macaque), M. thibetana (Tibetan macaque) and M. fuscata (Japanese macaque). Calb was found in cone photoreceptors and in a subset of bipolar cells...
August 28, 2018: Anatomia, Histologia, Embryologia
Liliana Rytel, Kamila Szymanska, Ignacy Gonkowski, Joanna Wojtkiewicz
The gastrointestinal (GI) tract is innervated by nerve processes derived from the intramural enteric neurons and neurons localized outside the digestive tract. This study analysed the neurochemical characterization of nerves in the wall of the porcine oesophagus using single immunofluorescence technique. Immunoreactivity to vesicular acetylcholine transporter (VAChT), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), somatostatin (SOM), galanin (GAL), neuronal isoform of nitric oxide synthase (nNOS), substance P (SP), leucine enkephalin (LENK), calcitonin gene-related peptide (CGRP) or dopamine beta-hydroxylase (DBH) was investigated in intramuscular and intramucosal nerves of the cervical, thoracic and abdominal oesophagus...
August 14, 2018: Anatomia, Histologia, Embryologia
Francisco Gil, Alberto Arencibia, Verónica García, Gregorio Ramírez, José María Vázquez
The aim of the study was to describe the anatomical location and the topographic relationship of the major salivary glands to associated tissues in six dog cadavers by high-field magnetic resonance imaging (MRI) and gross anatomical dissections and sections. Spin-echo T1-weighted and T2-weighted magnetic resonance (MR) images were obtained in transverse, sagittal, and dorsal planes with a 1.5 T magnet. The digastric muscle, the external auditory canal, the eyeball, and the medial pterygoid muscle were the most consistent topographic landmarks for the identification of the mandibular, parotid, and zygomatic salivary glands, respectively...
August 14, 2018: Anatomia, Histologia, Embryologia
Mohamed A M Alsafy, Samir A El-Gendy, Basma Kamal
The camel digits have a complex structure with tendons, joints and ligaments. The objective of the study was to determine the anatomical features of the metatarsophalangeal joint and its puncture site in dromedary camel using anatomical dissection, radiography, computed tomography (CT) bone and soft tissue window scans and 3D reconstruction render volume of CT. The study used six adult camels of both sexes (three males and three females), aged 9-15 years (Mean ± SD equal 11.80 ± 2.59 years). The camel fetlock possesses two metatarsophalangeal joints, left and right ones...
August 6, 2018: Anatomia, Histologia, Embryologia
Đorđe N Milićević, Sanja Z Despotović, Jürgen Westermann, Novica M Milićević
The spleen is the only blood filter in the organism which removes foreign antigens and effete cells from circulation. The significant role in capturing, transporting and presentation of antigens to immune cells is executed by a special subset of splenic macrophages called marginal metallophilic macrophages. Upon stimulation with lipopolysaccharide, these cells promptly migrate from their preferential location at the inner aspect of the splenic marginal sinus into the B-cell lymphoid follicles. This migration is executed via CXC chemokine ligand 13 in a lymphotoxin-dependent fashion...
August 6, 2018: Anatomia, Histologia, Embryologia
Mateusz K Hołda, Jakub Hołda, Mateusz Koziej, Kamil Tyrak, Wiesława Klimek-Piotrowska
AIM: The aim of this study was to investigate the influence of formaldehyde-based fixation on dimension, weight and shape of cardiac tissue during a 1-year observation. MATERIALS AND METHODS: Seven measuring sites were permanently marked in 40 swine hearts prior to fixation. Four study groups (n = 10 each) were assembled that differed only in concentration and the type of fixative. The fixatives were 2%, 4% or 10% formaldehyde phosphate-buffered solution (FPBS) and alcoholic formalin...
July 30, 2018: Anatomia, Histologia, Embryologia
Abdel-Hamid K Osman, Louise C Abbott, Amal A M Ahmed
Progesterone receptors (PR) are necessary to mediate the biological effects of progesterone and are integral to the regulation of a number of different aspects of reproduction in mammals including ovulation of the oocyte, implantation of the conceptus and maintenance of pregnancy. This study investigated the expression and localization of progesterone receptors in the uterine wall of both pregnant and cyclic (nonpregnant) camels. Uterine tissue samples were collected from healthy animals and processed for routine histological and immunohistochemical staining techniques to reveal nuclear PR...
July 29, 2018: Anatomia, Histologia, Embryologia
Toshiyuki Tanaka, Hideo Akiyoshi, Keiichiro Mie
The circle of Willis (CW) supplies blood to the brain. In humans, anatomical variations in the CW are an important risk factor for transient ischaemic attack and stroke. In canines, anatomical variations in the CW have not been reported. The aim of this study was to determine anatomical variations in the CW in dogs by magnetic resonance angiography. Normal configuration of the CW was observed in 82%, aplasia of the precommunicating segment of the rostral cerebral artery was observed in 14%, and aplasia of the rostral communicating artery was observed in 4% of dogs included in the study...
July 22, 2018: Anatomia, Histologia, Embryologia
Rafael C Siqueira, Sheila C Rahal, Leticia R Inamassu, Maria J Mamprim, Rodrigo H F Teixeira, Carlos R Teixeira, Fabricio B Rassy, Luciane R Mesquita, Frederico O B Monteiro, Marcilio Felix, Alessandra Melchert
The aim of this study was to determine some radiographic reference values for hip joint, femur and patella in maned wolves. Ten maned wolves (Chrysocyon brachyurus), five live (G1) and five cadavers (G2), were used. There were no statistically significant differences in the radiological measurements between right and left pelvic limbs of the G1 and G2, except for mechanical lateral proximal femoral angle (mLPFA) in both Groups. The comparison of the measurements between Groups showed difference only for mechanical lateral distal femoral angle (mLDFA)...
July 22, 2018: Anatomia, Histologia, Embryologia
Alexander V Makarevich, Martina Földešiová, Juraj Pivko, Elena Kubovičová, Peter Chrenek
Follicle atresia in mammals is a universal phenomenon characteristic by degenerative morphological changes in granulosa and theca cells. The unfavourable effect of milk production in relation to fertility has been studied starting from the 70s of the last century; however, there is no unambiguous and persuasive data on association of ovarian atresia with milk yield of dairy cows. The aim of this study was to define histological signs of ovarian follicle atresia in dairy cows in relation to their milk production...
July 18, 2018: Anatomia, Histologia, Embryologia
Ralph G Thomé, Isabella Cristina de Oliveira Cardoso, Sabrina Elisa de Oliveira, Hélio Batista Dos Santos
We determined for the first time the reproductive biology of Piabina argentea through macroscopic and microscopic analysis of ovaries and evaluated the morphological changes in hepatocytes. Two hundred and 46 specimens were collected, 204 females and 42 males, between March 2014 and February 2015. Biometrics data were obtained. From females, gonad and liver samples were conducted to histological, histochemical and immunohistochemical techniques. Mature ovaries were used to determine absolute and relative fecundity...
July 16, 2018: Anatomia, Histologia, Embryologia
Eva Patricia López-Damián, José Alfredo Jiménez-Medina, Miguel Angel Lammoglia, Jaime Arturo Pimentel, Lourdes Teresa Agredano-Moreno, Chris Wood, Carlos Salvador Galina, Tatiana Fiordelisio
Embryo transfer using cryopreserved B. indicus embryos results in low pregnancy rates. The low viability of B. indicus embryos is likely a result of their low freezability relative to embryos from other species, notably B. taurus. Freezability is probably related to the quantity of lipid droplets in the embryonic cells. However, the mechanism underlying this association is unknown. Using multiphoton and transmission electron microscopy, the proportion, volume, morphology and composition of lipid droplets in fresh and cryopreserved B...
July 5, 2018: Anatomia, Histologia, Embryologia
Manuel L Sánchez, Eliana de Souza, Luis A Aguilar, Rafael Coveñas
Using an immunocytochemical technique, we have studied in the alpaca brainstem the distribution of immunoreactive structures containing prodynorphin (alpha-neoendorphin)- and pro-opiomelanocortin (adrenocorticotrophin hormone (18-39) (ACTH), beta-endorphin (1-27))-derived peptides. No peptidergic-immunoreactive cell body was observed. Immunoreactive fibres were widely distributed, although in most of the brainstem nuclei the density of the peptidergic fibres was low or very low. In general, the distribution of the immunoreactive fibres containing the peptides studied was very similar...
October 2018: Anatomia, Histologia, Embryologia
Kongkiat Srisuwatanasagul, Nawarus Prapaiwan, Sayamon Srisuwatanasagul, Annop Kunavongkrit, Atthaporn Roongsitthichai
This study aimed to investigate the immunoexpression of Ki-67 protein, androgen receptor (AR), and estrogen receptor beta (ERβ) in testicular tissues of male pigs immunocastrated using GnRH vaccine (Improvac™, Zoetis Co., Ltd., Thailand) with different times. Totally, 30 male pigs were classified by castration protocol into three groups: T1 (n = 10) consisted of pigs immunocastrated at 14 and 18 weeks of age, T2 (n = 10) included pigs immunocastrated at 9 and 19 weeks of age, and C (n = 10) contained intact pigs...
October 2018: Anatomia, Histologia, Embryologia
Mike M Mpango, Mary-Cathrine Madekurozwa
This study describes ciliated, nonciliated and mitochondrial luminal epithelial cells of the isthmus in laying and moulting domestic fowls using histological and ultrastructural techniques. The ciliated cells were nonsecretory, while numerous electron-dense secretory granules were present in the nonciliated cells of laying birds. Mitochondrial cells, occurring in two morphologically distinct forms, constituted the third type of epithelial cell present in the isthmus. The SEM study showed that the luminal epithelium was dominated by ciliated cells, the cilia of which partially obscured adjacent nonciliated cells...
October 2018: Anatomia, Histologia, Embryologia
Olivia Garcia-Suarez, Roberto Cabo, Francesco Abbate, Basilio Randazzo, Rosaria Laurà, Giuseppe Piccione, Antonino Germanà, Maria Levanti
Leptin is an anorectic hormone secreted mainly by peripheral adipocytes but also by other central and peripheral tissues. It acts by means of a receptor called OB-R, influencing not only appetite and body mass but being also involved in many fields like endocrinology, metabolism and reproduction. Immunohistochemistry and qRT-PCR techniques were, respectively, used to demonstrate the presence of leptin and its receptor in the gut of adult zebrafish and to evaluate the leptin gene expression response to feeding and fasting...
October 2018: Anatomia, Histologia, Embryologia
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