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Tokai Journal of Experimental and Clinical Medicine

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https://www.readbyqxmd.com/read/30191548/using-artificial-dna-sequence-to-suppress-non-specific-bindings-in-crude-nuclear-extract-during-surface-plasmon-resonance-assay
#1
Zhong Lian Li, Kenta Nagahori, Shinichi Kawata, Takuya Omotehara, Yuki Ogawa, Hidenobu Miyaso, Masahiro Itoh, Yoshinori Otsuki, Kou Sakabe
OBJECTIVE: Surface plasmon resonance (SPR) has been extensively used to characterize the interactions between molecules in terms of their binding specificity, affinity, and kinetics. Practical procedures, however, for measurement of the protein-DNA association in crude nuclear extract are yet to be defined. METHODS: Crude nuclear extract was obtained from MCF-7 cells or recombinant estrogen receptor alpha (ERα) was used for analysis. To suppress signal from non-specific bindings in SPR assay using Biacore, DNA fragments with minimal protein binding activity were identified in a database for transcription factors and included in the study...
September 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/30191547/comparison-between-endoscopic-treatment-and-surgical-drainage-of-the-pancreatic-duct-in-chronic-pancreatitis
#2
Yohei Kawashima, Yoshiaki Kawaguchi, Aya Kawanishi, Masami Ogawa, Kenichi Hirabayashi, Toshio Nakagohri, Tetsuya Mine
BACKGROUND: Treatment of recurrent chronic obstructive pancreatitis is pancreatic duct decompression with endoscopic drainage (endoscopic pancreatic stenting [EPS] with extracorporeal shockwave lithotripsy [ESWL]) or surgical drainage. Despite the recent popularization of endoscopic drainage, treatment or stent removal is difficult in many patients. We compared the efficacy, safety, and medical cost of endoscopic and surgical treatments. PATIENTS AND METHODS: We retrospectively compared the treatment course and medical cost of hospitalization between 41 patients who had undergone pancreatic stenting between 2006 and 2010 (EPS group) and 10 patients who had undergone surgery for poor control of pancreatitis between 2001 and 2005 (surgical drainage group)...
September 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/30191546/a-case-control-study-on-the-risk-factors-for-ischemic-colitis
#3
Tetsufumi Uchida, Masashi Matsushima, Yasushi Orihashi, Makiko Dekiden-Monma, Hajime Mizukami, Fumio Nakahara, Jun Nakamura, Mia Fujisawa, Jun Koike, Takayoshi Suzuki, Tetsuya Mine
OBJECTIVE: Ischemic colitis (IC) is a relatively common acute inflammation disorder of the intestine. It was considered to be a disorder of elderly people with risk factors for arteriosclerosis; however, a considerable number of young people with IC have been reported recently. We performed a case-control study to determine the risk factors for IC and compare the risk factors between elderly and non-elderly people. METHODS: The study included 209 consecutive patients diagnosed with IC between December 2004 and March 2017 at Tokai University Hospital...
September 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/30191545/a-case-of-acute-respiratory-distress-syndrome-caused-by-waterproofing-spray-inhalation
#4
Tohru Sawamoto, Seiji Morita, Yasue Watanabe, Asami Omata, Ryo Suzuki, Masato Tsuchikane, Sadaki Inokuchi
We report the case of a 62-year-old man who used approximately one can of waterproofing spray in an enclosed room and, then, smoked a cigarette. He developed a fever of 39°C with respiratory distress and was transported by ambulance to his usual doctor. Since his respiratory state was very severe, he was transferred to our hospital. The patient had a smoking habit of 20 cigarettes per day for approximately 42 years. Chest computed tomography (CT) on arrival showed ground glass opacity (GGO) in the bilateral lungs with emphysematous change...
September 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/30191544/a-young-patient-with-emery-dreifuss-muscular-dystrophy-treated-with-endovascular-therapy-for-cardioembolic-stroke-a-case-report
#5
Kazunari Homma, Eiichiro Nagata, Hideyuki Hanano, Tsuyoshi Uesugi, Yoichi Ohnuki, Shinichi Matsuda, Shoji Kazahari, Shunya Takizawa
We had a case of Emery-Dreifuss muscular dystrophy (EDMD) in an 18-year-old woman who underwent endovascular therapy for a cardioembolic stroke. At 5 years old, she showed a high creatine kinase level and atrial fibrillation on electrocardiography in our hospital. Finally, she was diagnosed as having EDMD by genetic screening that revealed mutations in the LMNA gene (c.810+1G>T). Before this event, she received no medications. At 18 years old, she was admitted to our hospital>8 hours after the onset of sudden consciousness disturbance...
September 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/30191543/detection-of-autonomic-nervous-system-abnormalities-in-diabetic-patients-by-24-hour-ambulatory-blood-pressure-monitoring
#6
Moritsugu Kimura, Masao Toyoda, Moe Ono, Nobumichi Saito, Noriko Kaneyama, Masaaki Miyauchi, Tomoya Umezono, Masafumi Fukagawa
OBJECTIVE: To determine the relationship between 24-hr blood pressure (BP) fluctuations and autonomic nervous system dysfunction in diabetic patients using non-invasive ambulatory blood-pressure monitoring (ABPM) system. METHODS: The subjects were 39 diabetic patients free of cardiovascular diseases. 24-hr BP was monitored by a non-invasive ABPM system. The relationships among 24-hr BP fluctuations and various clinical parameters relevant to diabetes and hypertension were analyzed...
September 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/30191542/effects-of-cysteine-stabilized-peptide-fraction-of-morinda-lucida-leaf-on-selected-kidney-function-indices-in-mice
#7
Kayode Ezekiel Adewole, Joseph Oluwatope Adebayo
OBJECTIVE: This study evaluated the effect of cysteine-stabilized peptide fraction (CSPF) of Morinda lucida leaf on selected kidney function indices in mice. METHODS: Sixty mice were assigned into six groups. Group A served as the control while groups B, C, D, E and F received 31.25, 61.5, 125, 250, and 500 mg/kg body weight of CSPF respectively for 7 or 28 days. RESULTS: Administration of CSPF for 7 and 28 days caused no significant (p>0...
September 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/30191541/impact-of-adding-bevacizumab-to-paclitaxel-carboplatin-for-platinum-sensitive-recurrent-epithelial-ovarian-cancer-a-propensity-score-matching-analysis
#8
Takeshi Hirasawa, Hiroko Machida, Tetsuji Iida, Masae Ikeda, Masako Shida, Mikio Mikami
PURPOSE: We evaluated whether adding bevacizumab (Bev) to paclitaxel+carboplatin (TC) could improve outcomes, especially progression-free survival (PFS), in patients with platinum-sensitive recurrent ovarian cancer. PATIENTS AND METHODS: Among patients with platinum-sensitive recurrent ovarian cancer treated at our hospital from May 2008 to March 2017, PFS was compared between those receiving platinum-based chemotherapy or TC+Bev therapy by propensity score matching analysis...
September 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/30191540/recurrent-small-cell-carcinoma-of-the-uterine-cervix-responding-to-combined-therapy-with-paclitaxel-cisplatin-and-bevacizumab-a-case-report
#9
Takeshi Hirasawa, Hiroshi Kajiwara, Hiroko Machida, Tetsuji Iida, Masae Ikeda, Masako Shida, Mikio Mikami
We report a patient with recurrent refractory small cell carcinoma of the uterine cervix, in whom combined therapy with paclitaxel, cisplatin, and bevacizumab (TP+Bev) was effective. Small cell cervical carcinoma is a rare malignancy and its outcome is reported to be poor. The patient was a 45-year-old woman who visited a local hospital with the chief complaint of irregular vaginal bleeding and was referred to our department. The results of a smear test and examination of a tissue biopsy specimen suggested small cell carcinoma...
September 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29961936/clinical-application-of-high-pitched-breath-sound-in-children-with-asthma
#10
Yoshimi Kondo, Mariko Nukaga, Mayumi Enseki, Hideyuki Tabata, Kota Hirai, Masahiko Kato, Hiroyuki Mochizuki
OBJECTIVE: Breath sound parameters have been suggested to be a new biomarker of airway conditions in asthmatic patients. We investigated new breath sound parameters to determine their utility for evaluating asthmatic children. METHODS: Fifty-seven children (mean age, 9.0 years, 6-16 years) were included in the present study. The new breath sound parameters, the area under the curve (AUC)>1,000 Hz (A1000 [dBm·Hz]) and the ratio of the A1000 to the total AUC at 100 Hz to the highest frequency of the dBm power spectrum (AT ) (A1000 /AT [%]) were measured before and after β2 agonist inhalation...
July 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29961935/clinical-reasoning-in-kampo-education-for-teaching-kampo-beginners
#11
Yoshinobu Nakada, Noriyuki Takano, Makoto Arai
OBJECTIVES: Japanese traditional (Kampo) medicine has its own theories that are quite different from those of Western medicine. For many students and medical doctors, mastering it is a painstaking task. We examined the similarities in clinical reasoning between Western and Kampo medicine, and developed an easy-to-understand method to teach Kampo theories enabling physicians to make accurate diagnoses and choose suitable Kampo formulae. METHODS: We developed a teaching method for Kampo medicine along clinical reasoning for beginners and evaluate its availability and effectiveness in an actual team-based learning class...
July 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29961934/symptomatic-lacunar-infarct-accompanied-with-posterior-reversible-encephalopathy-syndrome-a-case-report
#12
Shoji Kazahari, Kazunari Honma, Reina Kawamura, Tsuyoshi Uesugi, Eiichiro Nagata, Shunya Takizawa
We present a 48-year-old man with a history of hypertension, who suddenly noticed dysarthria and right hemiparesis. Diffusion-weighted MRI at 1 day after the onset showed a small high-intensity region in the left corona radiata, indicating the acute phase of lacunar infarction. Fluid attenuation inversion recovery images showed extensive hyperintense lesions predominantly in the white matter of the fronto-temporoparietal lobes and pons, indicating posterior reversible encephalopathy syndrome (PRES). In addition, T2*-weighted gradient-echo images showed multiple small round hypointense lesions in white matter and basal ganglia, indicating cerebral microbleeds...
July 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29961933/investigations-into-the-risk-of-reproductive-toxicity-following-exposure-to-highly-active-anti-retroviral-drugs-in-rodents
#13
O Awodele, T D Popoola, O Idowu, B M Bashua, N A Awolola, W O Okunowo
With the introduction of Highly Active Antiretroviral Therapy (HAART), there has been drastic decline in morbidity and mortality associated with HIV/AIDS. However, many patients experience adverse drug reactions perhaps due to the inherent toxic nature of HAART. The possible toxic effect of HAART (combination ARVs) on reproduction and sexual dysfunction in seropositive HIV patients remains a subject of intense research. This study was designed to investigate the toxic effects of HAART on the reproductive hormones and organs of male and female rats...
July 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29961932/anesthetic-management-in-tracheal-dilatation-for-severe-tracheal-stenosis
#14
Kenji Ito, Kai Yamazaki, Takugi Kan, Shuhei Tetsu, Keiichiro Saito, Mitsutomo Kohno, Masayuki Iwazaki, Toshiyasu Suzuki
We report the anesthetic management of a 65-year-old woman with recurrent, severe tracheal stenosis who underwent tracheal dilatation. She had visited the Department of Respiratory Medicine at our hospital for respiratory distress approximately 20 years ago, and had undergone laser ablation under local anesthesia. Because of recurrence and aggravation of respiratory distress, she now presented at the Department of Thoracic Surgery, and was scheduled for surgery. Percutaneous cardiopulmonary support was prepared, and she was sedated with midazolam and dexmedetomidine...
July 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29961931/qualitative-study-on-the-psychological-experiences-of-trans-man-and-trans-woman
#15
Takeshi Sudo, Kenji Yamamoto, Reiko Yoshikawa
Transsexuals have problems associated with self and gender identities, as well as with their relationships with specialists such as doctor and counsellors. On the basis of the principle of interpretative phenomenological analysis, an exploratory research was carried out on self by studying the psychological experiences of participants and their relationships with specialists. Consequently the following needs of the transsexuals were identified: (1) understanding and alleviation of grief, conflict, and anxiety associated with gender dysphoria; (2) recovery and improvement of self-esteem; (3) support in real-life experiences and measures related to social and legal issues, such as coming out; and (4) necessity of information provision at medical institutions and among peers...
July 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29961930/rectal-neuroendocrine-tumor-with-synchronous-pancreatic-metastasis-a-case-report
#16
Yoshihito Masuoka, Daisuke Furukawa, Naoki Yazawa, Hideki Izumi, Misuzu Yamada, Taro Mashiko, Gota Saito, Kazutake Okada, Akira Tanaka, Toshiyuki Suzuki, Sotaro Sadahiro, Kenichi Hirabayashi, Toshio Nakagohri
INTRODUCTION: Gastrointestinal neuroendocrine tumors (GI-NETs) often show hematogenous metastasis, with the liver being the most common metastatic site; however, metastasis to the pancreas is rare. CASE PRESENTATION: We report a rare case of rectal NETs with pancreatic metastases in a 75-year-old man who presented with a chief complaint of constipation. Imaging and endoscopic findings revealed a rectal submucosal tumor, a pancreatic hypovascular mass, and multiple liver masses...
July 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29637537/right-aortic-arch-with-mirror-image-branching-in-adults-evaluation-using-ct
#17
Toshihiko Hayashi, Tamaki Ichikawa, Hiroshi Yamamuro, Shun Ono, Makiko Kobayashi, Takakiyo Nomura, Kazunobu Hashida, Hideki Yashiro, Tomohisa Okochi, Jun Koizumi, Shinichiro Shimura, Terumitsu Hasebe, Yutaka Imai
OBJECTIVE: We evaluated radiological findings and clinical significance of right aortic arch with mirror-image branching (RAMI) in adults using data from computed tomography (CT) examinations. MATERIAL AND METHODS: We reviewed recorded reports and CT images obtained from university and branch hospitals for RAMI in adults. The RAMI incidence in adults found on CT was assessed. Associated congenital and acquired cardiovascular diseases were evaluated. RESULTS: A total of 27 cases (14 men, 13 women; mean age, 59...
April 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29637536/interaction-of-inos-gene-c150t-polymorphism-and-endothelial-dysfunction-in-pathophysiology-of-metabolic-syndrome
#18
Ashok Kumar Ahirwar, Anju Jain, Archana Singh, Shilpa Bhardwaj, Binita Goswami, M K Bhatnagar, Jayashree Bhattacharjee
OBJECTIVE: To study the endothelial dysfunction by measuring Nitric Oxide and Endothelin-1, and inter-genotypic variation of inducible Nitric Oxide Synthase gene (C150T) polymorphism among the study subjects. METHODS: 50 diagnosed cases of metabolic syndrome as per International Diabetes Federation (IDF) criteria and 50 healthy volunteers as control were enrolled. Nitric Oxide, Endothelin were measured and PCR-RFLP was done to identify the iNOS gene C150T polymorphism and its effect on serum nitric oxide levels...
April 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29637535/raltegravir-associated-diabetic-ketoacidosis-in-a-patient-with-hiv-infection-a-case-report
#19
Mari Horikawa, Masao Toyoda, Nobumichi Saito, Moritsugu Kimura, Takako Kobayashi, Atsushi Takagi, Masafumi Fukagawa
Antiretroviral drugs, especially protease inhibitors (PI), are known to induce disorders of lipid and glucose metabolism. However, there are only a few reports of these side effects in patients treated with integrase strand transfer inhibitors (INSTI). We encountered the case of a 46-year-old man who had been treated for type 2 diabetes with diet and exercise. He contracted immunodeficiency virus (HIV) infection two years earlier and received highly active antiretroviral therapy (HAART). Three months before the current admission, HAART was switched from a non-nucleic acid reverse transcriptase inhibitor (NNRTI) to an INSTI (raltegravir: 800 mg/day)...
April 20, 2018: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29637534/utility-of-a-3d-roadmap-during-balloon-occluded-retrograde-transvenous-obliteration-for-gastric-varices
#20
Yoshimi Fujii, Jun Koizumi, Takuya Hara, Tatsuya Sekiguchi, Syun Ono, Tetsuya Mine, Tatehiro Kagawa, Shunji Hirose, Kota Tsuruya, Kazuya Anzai, Bertrand Janne d'Othee, Yutaka Imai
OBJECTIVE: We describe our initial clinical experience regarding the use of a 3D roadmap during balloon-occluded retrograde transvenous obliteration (BRTO) in three patients. METHODS: Between June 2016 and July 2016, three BRTO procedures were performed in three patients with gastric varices. Preprocedural intravenous dynamic CT was performed, and portal venous phase CT images were postprocessed to obtain volume rendering (VR) images. A 3D roadmap was developed by overlaying the VR images onto the real-time X-ray fluoroscopy images...
April 20, 2018: Tokai Journal of Experimental and Clinical Medicine
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