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Investigative Ophthalmology & Visual Science

Tawna L Roberts, Kristi N Kester, Richard W Hertle
Purpose: This study presents test-retest reliability of optotype visual acuity (OVA) across 60° of horizontal gaze position in patients with infantile nystagmus syndrome (INS). Also, the validity of the metric gaze-dependent functional vision space (GDFVS) is shown in patients with INS. Methods: In experiment 1, OVA was measured twice in seven horizontal gaze positions from 30° left to right in 10° steps in 20 subjects with INS and 14 without INS. Test-retest reliability was assessed using intraclass correlation coefficient (ICC) in each gaze...
April 1, 2018: Investigative Ophthalmology & Visual Science
Xiu-Mei Yang, Chun-Guang Duan, Jian Zhang, Xiao-Jie Qu, Yu-Sheng Wang
Purpose: Vasculogenesis has been shown to contribute to the formation of choroidal neovascularization (CNV). However, the mechanism behind the recruitment of endothelial progenitor cells (EPC) to CNV is not well understood. Therefore, we were interested to know whether integrin-linked kinase (ILK) plays a role in recruiting EPC to CNV, and its possible mechanism. Methods: We investigated the effect of hypoxia on retinal pigment epithelium (RPE) cells expressing ILK, hypoxia-inducible factor 1α (HIF-1α), stromal-derived factor-1 (SDF-1), and vascular endothelial growth factor (VEGF), and we further examined the effect of ILK small interfering RNA (siRNA) on their expression...
April 1, 2018: Investigative Ophthalmology & Visual Science
Chantal Dysli, Kaspar Schürch, Escher Pascal, Sebastian Wolf, Martin S Zinkernagel
Purpose: We investigated whether fundus autofluorescence (FAF) lifetimes in patients with retinitis pigmentosa display a disease-specific lifetime pattern. Methods: Fundus autofluorescence lifetime imaging ophthalmoscopy (FLIO) was performed in two spectral channels (498-560 and 560-720 nm) after excitation with a 473 nm pulsed laser in patients with retinitis pigmentosa and compared to healthy controls of a similar age range. Corresponding FAF intensity and spectral domain optical coherence tomography (OCT) data, as well as best corrected visual acuity (BCVA) were acquired and compared to fluorescence lifetime data...
April 1, 2018: Investigative Ophthalmology & Visual Science
David W Collins, Harini V Gudiseva, Venkata R M Chavali, Benjamin Trachtman, Meera Ramakrishnan, William T Merritt Iii, Maxwell Pistilli, Rebecca A Rossi, Stephanie Blachon, Prithvi S Sankar, Eydie Miller-Ellis, Amanda Lehman, Victoria Addis, Joan M O'Brien
Purpose: We investigate the function of the V83I polymorphism (m.6150G>A, rs879053914) in the mitochondrial cytochrome c oxidase subunit 1 (MT-CO1) gene and its role in African American (AA) primary open-angle glaucoma (POAG). Methods: This study used Sanger sequencing (1339 cases, 850 controls), phenotypic characterization of Primary Open-Angle African American Glaucoma Genetics study (POAAGG) cases, a masked chart review of CO1 missense cases (V83I plus M117T, n = 29) versus wild type cases (n = 29), a yeast 2-hybrid (Y2H) cDNA library screen, and quantification of protein-protein interactions by Y2H and ELISA...
April 1, 2018: Investigative Ophthalmology & Visual Science
Carolina Lema, Rose Y Reins, Rachel L Redfern
Purpose: To determine high-mobility group box 1 (HMGB1) expression during experimental dry eye (EDE) and dry eye-like culture conditions and elucidate its role in corneal dry eye-related inflammation. Methods: EDE was induced in 8- to 12-week-old C57BL/6 mice. Corneal tissue sections and lysates from EDE and untreated mice were evaluated for HMGB1 expression by immunostaining and quantitative real-time PCR (qPCR). For in vitro studies, human corneal epithelial cells (HCEC) were treated with hyperosmolar media, toll-like receptor (TLR) agonists, or proinflammatory cytokines to determine HMGB1 expression...
April 1, 2018: Investigative Ophthalmology & Visual Science
Srikant K Sahu, Sharad K Mittal, William Foulsham, Mingshun Li, Virender S Sangwan, Sunil K Chauhan
Purpose: The purpose of this study was to investigate the contribution of mast cells to early neutrophil recruitment during ocular inflammation. Methods: In a murine model of corneal injury, the epithelium and anterior stroma were removed using a handheld motor brush. Cromolyn sodium (2% in PBS) eye drops were administered topically for mast cell inhibition. In vitro, bone marrow-derived mast cells were cultured alone or with corneal tissue. The frequencies of CD45+ inflammatory cells, CD11b+Ly6G+ neutrophils, and ckit+FcεR1+ mast cells in the cornea were assessed by flow cytometry...
April 1, 2018: Investigative Ophthalmology & Visual Science
Sunao Sugita, Kenichi Makabe, Yuko Iwasaki, Shota Fujii, Masayo Takahashi
Purpose: To determine whether human induced pluripotent stem (iPS) cell-derived retinal pigment epithelial (RPE) cells (iPS-RPE) can suppress natural killer (NK) cell activation. Methods: iPS-RPE cells were cocultured with peripheral blood mononuclear cells (PBMCs) or purified NK cells from healthy donors after stimulation with cytokines. To confirm expression of NK cell-specific markers, flow cytometry and quantitative RT-PCR (qRT-PCR) were performed. NK cells (or PBMCs) cocultured with iPS-RPE cells were assessed for proliferation by Ki-67 expression with flow cytometry, and NK suppression by RPE cells was assessed for granzyme B production with ELISA...
April 1, 2018: Investigative Ophthalmology & Visual Science
Yeni H Yucel, Emily Mathieu, Neeru Gupta
No abstract text is available yet for this article.
April 1, 2018: Investigative Ophthalmology & Visual Science
Peter Wostyn, Veva De Groot, Debby Van Dam, Kurt Audenaert, Peter Paul De Deyn, Hanspeter Esriel Killer
No abstract text is available yet for this article.
April 1, 2018: Investigative Ophthalmology & Visual Science
Ganesh Prasanna, Luciana Ferrara, Christopher Adams, Takeru Ehara, Byron Li, Louis Yang, Chuanxi Xiang, Christopher Thow Hing Ng, Sean Kim, Christopher Towler, Todd Topley, Cale McAllister, Malay Ghosh, Ronald Newton, Rebecca Stacy, Dennis S Rice, Muneto Mogi
Purpose: The nitric oxide/soluble guanylate cyclase/protein kinase G (NO/sGC/PKG) is known to be involved in the regulation of intraocular pressure (IOP) and may be dysregulated in glaucoma. The purpose is to demonstrate that the sGC activator MGV354 lowers IOP in a monkey model of glaucoma and could be considered as a possible new clinical drug candidate. Methods: Changes to cGMP were assessed in primary human trabecular meshwork (hNTM) cells and binding studies were conducted using human sGC full-length protein...
April 1, 2018: Investigative Ophthalmology & Visual Science
Nayuta Yoshioka, Barbara Zangerl, Jack Phu, Agnes Y J Choi, Sieu K Khuu, Katherine Masselos, Michael P Hennessy, Michael Kalloniatis
Purpose: To investigate the effect of stimulus size and disease status on the structure-function relationship within the central retina, we correlated the differential light sensitivity (DLS) with Goldmann stimulus size I to V (GI-V) and optical coherence tomography (OCT) derived in vivo ganglion cell count per stimulus area (GCc) within the macular area in normal subjects and patients with early glaucoma. Methods: Humphrey Field Analyzer 10-2 visual field data with GI through V and Spectralis OCT macular ganglion cell layer (GCL) thickness measurements were collected from normal and early glaucoma cohorts including 25 subjects each...
April 1, 2018: Investigative Ophthalmology & Visual Science
Iben Bach Damgaard, Anders Ivarsen, Jesper Hjortdal
Purpose: To examine the refractive correction and corneal biomechanical strength after small incision lenticule extraction (SMILE) by using a 110- or 160-μm cap thickness. Methods: Thirty-two human donor corneas were allocated into 4 groups, combining one of two cap thicknesses (110 and 160 μm) with one of two spherical corrections (-4 D and -8 D). Each cornea was mounted on an artificial anterior chamber. The chamber pressure was adjusted by an attached 8% dextran media column...
April 1, 2018: Investigative Ophthalmology & Visual Science
Soufiane Ajana, Daniela Weber, Catherine Helmer, Bénédicte M Merle, Wolfgang Stuetz, Jean-François Dartigues, Marie-Bénédicte Rougier, Jean-François Korobelnik, Tilman Grune, Cécile Delcourt, Catherine Féart
Purpose: We investigated the cross-sectional associations between macular pigment optical density (MPOD), plasma lutein (L), and zeaxanthin (Z) concentrations and cognitive function in 184 older adults of the 3-City-Bordeaux cohort. Methods: MPOD was measured using the two-wavelength autofluorescence method with a modified scanning laser ophthalmoscope. Plasma L and Z (L+Z) concentrations were determined by high-performance liquid chromatography and were considered either crude or expressed as a ratio of the concentration of plasma lipids (total cholesterol [TC] + triglycerides [TG])...
April 1, 2018: Investigative Ophthalmology & Visual Science
Sungsoon Hwang, Dong Hui Lim, Soomin Lee, Daye Diana Choi, Eui-Sang Chung, Tae-Young Chung
Purpose: To report the long-term results of temporary piggyback IOL implantation in congenital cataract and to compare the clinical outcomes of temporary piggyback IOL with those of single IOL implantation. Methods: This is a retrospective, comparative, interventional study. The medical records of all consecutive patients who underwent cataract extraction and single or temporary piggyback IOL implantation within the first 3 years of life from 1999 to 2013 at Samsung Medical Center were reviewed...
April 1, 2018: Investigative Ophthalmology & Visual Science
Yan Wang, Heming Li, Shihu Zang, Fanfan Li, Yingying Chen, Xiao Zhang, Zongming Song, Qing Peng, Feng Gu
Purpose: To identify the causative gene and investigate the corresponding mechanisms for an autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV) family. Methods: Clinical examination and genetic analysis were performed in a Chinese ADNIV family. To dissect the molecular consequence, we used gene targeting to knock-in a patient's specific mutation in the mouse genome. Immunostaining and immunoprecipitation were harnessed to analyze the colocalization and interaction of CAPN5 with SLIT2 in photoreceptors...
April 1, 2018: Investigative Ophthalmology & Visual Science
Xiaoxuan Yang, Lizhen Wang, Lunhao Li, Zhang Yu, Caiwen Xiao
Purpose: To explore the quantitative distributions of different lymphocyte subsets in the lacrimal sac mucosa and identify changes of Th1- and Th2-associated cytokines in the tears of patients with chronic dacryocystitis. Methods: Lacrimal sac mucosal specimens from patients with chronic dacryocystitis were analyzed. Hematoxylin-eosin staining and Masson staining were performed for pathologic analysis, and immunohistochemical staining was performed for the detection of CD3+, CD4+, CD8+, CD20+, Th1, and Th2 lymphocytes...
April 1, 2018: Investigative Ophthalmology & Visual Science
(no author information available yet)
No abstract text is available yet for this article.
April 1, 2018: Investigative Ophthalmology & Visual Science
(no author information available yet)
No abstract text is available yet for this article.
April 1, 2018: Investigative Ophthalmology & Visual Science
Angelica Ly, Lisa Nivison-Smith, Nagi Assaad, Michael Kalloniatis
Purpose: To develop a proof-of-concept, computational method for the quantification and classification of fundus images in intermediate age-related macular degeneration (AMD). Methods: Multispectral, unsupervised pattern recognition was applied to 184 fundus images from 10 normal and 36 intermediate AMD eyes. The imaging results of preprocessed, grayscale images from three modalities (infrared, green, and fundus autofluorescence scanning laser ophthalmoscopy) were automatically classified into various clusters sharing a common spectral signature, using a k-means clustering algorithm...
April 1, 2018: Investigative Ophthalmology & Visual Science
Moritz Lindner, Sebastian Kosanetzky, Maximilian Pfau, Jennifer Nadal, Lukas A Gördt, Steffen Schmitz-Valckenberg, Matthias Schmid, Frank G Holz, Monika Fleckenstein
Purpose: To assess the impact of distinct atrophy border characteristics based on spectral-domain optical coherence tomography (SD-OCT) imaging on local atrophy progression. Methods: Patients with geographic atrophy (GA) secondary to AMD were recruited in the context of the Longitudinal Fundus Autofluorescence in Age-related Macular Degeneration and Directional Spread in Geographic Atrophy studies (NCT00393692, NCT02051998). Horizontal and vertical SD-OCT scans were acquired at sequential visits using a device allowing for anatomically accurate registration of follow-up to baseline scans...
March 20, 2018: Investigative Ophthalmology & Visual Science
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