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Journal of Bioenergetics and Biomembranes

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https://www.readbyqxmd.com/read/28078625/analysis-of-an-n-terminal-deletion-in-subunit-a-of-the-escherichia-coli-atp-synthase
#1
Robert R Ishmukhametov, Jessica DeLeon-Rangel, Shaotong Zhu, Steven B Vik
Subunit a is a membrane-bound stator subunit of the ATP synthase and is essential for proton translocation. The N-terminus of subunit a in E. coli is localized to the periplasm, and contains a sequence motif that is conserved among some bacteria. Previous work has identified mutations in this region that impair enzyme activity. Here, an internal deletion was constructed in subunit a in which residues 6-20 were replaced by a single lysine residue, and this mutant was unable to grow on succinate minimal medium...
January 11, 2017: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/28070860/energetic-oxidative-and-ionic-exchange-in-rat-brain-and-liver-mitochondria-at-experimental-audiogenic-epilepsy-krushinsky-molodkina-model
#2
Natalya I Venediktova, Olga S Gorbacheva, Natalia V Belosludtseva, Irina B Fedotova, Natalia M Surina, Inga I Poletaeva, Oleg V Kolomytkin, Galina D Mironova
The role of brain and liver mitochondria at epileptic seizure was studied on Krushinsky-Molodkina (KM) rats which respond to sound with an intensive epileptic seizure (audiogenic epilepsy). We didn't find significant changes in respiration rats of brain and liver mitochondria of KM and control rats; however the efficiency of АТР synthesis in the KM rat mitochondria was 10% lower. In rats with audiogenic epilepsy the concentration of oxidative stress marker malondialdehyde in mitochondria of the brain (but not liver) was 2-fold higher than that in the control rats...
January 9, 2017: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/28054274/nicorandil-improves-post-fatigue-tension-in-slow-skeletal-muscle-fibers-by-modulating-glutathione-redox-state
#3
E Sánchez-Duarte, X Trujillo, C Cortés-Rojo, A Saavedra-Molina, G Camargo, L Hernández, M Huerta, R Montoya-Pérez
Fatigue is a phenomenon in which force reduction has been linked to impairment of several biochemical processes. In skeletal muscle, the ATP-sensitive potassium channels (KATP) are actively involved in myoprotection against metabolic stress. They are present in sarcolemma and mitochondria (mitoKATP channels). K(+) channel openers like nicorandil has been recognized for their ability to protect skeletal muscle from ischemia-reperfusion injury, however, the effects of nicorandil on fatigue in slow skeletal muscle fibers has not been explored, being the aim of this study...
January 4, 2017: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27999981/phospholipids-in-mitochondrial-dysfunction-during-hemorrhagic-shock
#4
REVIEW
Galina F Leskova
Energy deficiency plays a key role in the development of irreversible shock conditions. Therefore, identifying mitochondrial functional disturbances during hemorrhagic shock should be considered a prospective direction for studying its pathogenesis. Phospholipid (PL)-dependent mechanisms of mitochondrial dysfunction in the brain (i.e., in the frontal lobes of the cerebral hemispheres and medulla oblongata) and liver, which, when damaged, leads to an encephalopathy, are examined in this review. These mechanisms show strong regional specificity...
December 20, 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27914013/inhibitory-effect-of-homocysteine-on-rat-neural-stem-cell-growth-in-vitro-is-associated-with-reduced-protein-levels-and-enzymatic-activities-of-aconitase-and-respiratory-complex-iii
#5
Xu-Mei Zhang, Ya-Qian Zhao, Hai Yan, Huan Liu, Guo-Wei Huang
Increased blood plasma concentration of the sulphur amino acid homocysteine (Hcy) is considered as an independent risk factor of the neurodegenerative diseases. However, the detailed molecular mechanisms by which Hcy leads to neurotoxicity have yet to be clarified. Recent research has suggested that neurotoxicity of Hcy may involve negative regulation of neural stem cell (NSC) proliferation. In the current study, primary NSCs were isolated from neonatal rat brain hippocampus and the inhibition in cell growth was observed after exposure to l50 μM and 500 μM L-Hcy...
December 2, 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27889841/the-function-of-activatable-cell-penetrating-peptides-in-human-intrahepatic-bile-duct-epithelial-cells
#6
Kui Tu, Lijin Zhao, Jin Gu, Pengzhan Yan, Fang Wang, Yu Cao
This study aimed to investigate the function of Activatable Cell-Penetrating Peptides (ACPP) in detecting the changes of human intrahepatic bile duct epithelial cell(hIBDEC). ACPP, which target matrix metalloproteinases, were constructed. All were labeled with FITC and Gd-DTPA at the N-terminal. Fluorescence microscopy was used to observe the fluorescence intensity inside hIBDEC after stimulating with different concentrations of LPS and incubating with different concentrations of ACPP to determine the optimal concentration range for LPS stimulation and the optimal concentration for FITC-ACPP effect...
December 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27878645/the-synaptojanins-in-the-murine-small-and-large-intestine
#7
María Dolores Vázquez-Carretero, Ana Eloisa Carvajal, José Manuel Serrano-Morales, Pablo García-Miranda, Anunciación Ana Ilundain, María José Peral
The expression of the phosphoinositides phosphatases Synaptojanins (Synjs) 1 and 2 has been shown in brain and in some peripheral tissues, but their expression in the intestine has not been reported. Herein we show that the small and large intestine express Synj1 and Synj2. Their mRNA levels, measured by RT-PCR, are not affected by development in the small intestine but in the colon they increase with age. Immunostaining assays reveal that both Synjs localize at the apical domain of the epithelial cells and at the lamina propria at sites also expressing the neuron marker calretinin...
December 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27687874/response-to-the-letter-to-the-editor-mitochondria-isolated-from-the-striatum-of-the-brain-exhibit-a-higher-degree-of-oxidative-phosphorylation-coupling-which-shows-that-they-are-not-subject-to-energetic-dysfunction-upon-acute-paraquat-administration
#8
EDITORIAL
https://www.readbyqxmd.com/read/27687873/mitochondria-isolated-from-the-striatum-brain-of-acute-paraquat-treated-rats-exhibit-a-higher-degree-of-oxidative-phosphorylation-coupling-which-shows-that-they-are-not-subject-to-energetic-dysfunction-upon-acute-paraquat-administration
#9
EDITORIAL
https://www.readbyqxmd.com/read/27683242/kinetic-and-mutational-studies-of-the-adenosine-diphosphate-ribose-hydrolase-from-mycobacterium-tuberculosis
#10
Suzanne F O'Handley, Puchong Thirawatananond, Lin-Woo Kang, Jennifer E Cunningham, J Alfonso Leyva, L Mario Amzel, Sandra B Gabelli
Mycobacterium tuberculosis represents one of the world's most devastating infectious agents - with one third of the world's population infected and 1.5 million people dying each year from this deadly pathogen. As part of an effort to identify targets for therapeutic intervention, we carried out the kinetic characterization of the product of gene rv1700 of M. tuberculosis. Based on its sequence and its structure, the protein had been tentatively identified as a pyrophosphohydrolase specific for adenosine diphosphate ribose (ADPR), a compound involved in various pathways including oxidative stress response and tellurite resistance...
December 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27683241/sex-dependent-alterations-in-mitochondrial-electron-transport-chain-proteins-following-neonatal-rat-cerebral-hypoxic-ischemia
#11
T G Demarest, R A Schuh, E L Waite, J Waddell, M C McKenna, Gary Fiskum
Males are more susceptible to brain mitochondrial bioenergetic dysfunction following neonatal cerebral hypoxic-ischemia (HI) than females. Mitochondrial biogenesis has been implicated in the cellular response to HI injury, but sex differences in biogenesis following HI have not been described. We tested the hypothesis that mitochondrial biogenesis or the expression of mitochondrial electron transport chain (ETC) proteins are differentially stimulated in the brains of 8 day old male and female rats one day following HI, and promoted by treatment with acetyl-L-carnitine (ALCAR)...
December 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27640193/differential-sensitivity-of-hepatocellular-carcinoma-cells-to-suppression-of-hepatocystin-transcription-under-hypoxic-conditions
#12
Jeong-Ju Yoo, Dong Hyeon Lee, Yuri Cho, Eun Ju Cho, Jeong-Hoon Lee, Su Jong Yu, Yoon Jun Kim, Chung Yong Kim, Jung-Hwan Yoon
Mutations in the gene encoding hepatocystin/80 K-H (PRKCSH) cause autosomal dominant polycystic liver disease. Hepatocystin deficiency impairs glucosidase II activity, which is critical for processing and folding glycoproteins in the endoplasmic reticulum (ER). Hypoxia is known as a strong stimulus for generating survival signals in hepatocellular carcinoma (HCC) cells. However, hypoxia may induce cell apoptosis under conditions of severe ER stress. Thus, we hypothesized that suppression of hepatocystin transcription induces HCC cell death under hypoxic conditions due to excessive ER stress...
December 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27714488/editorial-note
#13
Pablo M Peixoto, Evgeny Pavlov, Elizabeth Jonas
No abstract text is available yet for this article.
October 6, 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27896503/effect-of-pgc-1%C3%AE-overexpression-or-silencing-on-mitochondrial-apoptosis-of-goat-luteinized-granulosa-cells
#14
Guo-Min Zhang, Ming-Tian Deng, Yan-Li Zhang, Yi-Xuan Fan, Yong-Jie Wan, Hai-Tao Nie, Zi-Yu Wang, Feng Wang, Zhi-Hai Lei
During goat follicular development, abnormal expression of peroxisome proliferator- activated receptor gamma coactivator-1 alpha (PGC-1α) in granulosa cells (GCs) may contribute to follicular atresia with unknown regulatory mechanisms. In this study, we investigate the effect of ectopic expression or interference of PGC-1α on cell apoptosis of goat first passage granulosa cells (FGCs) in vitro. The results indicate that PGC-1α silencing by short hairpin RNA (shRNA) in goat FGCs significantly reduced mitochondrial DNA (mtDNA) copy number (P < 0...
October 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27854030/simple-oxygraphic-analysis-for-the-presence-of-adenylate-kinase-1-and-2-in-normal-and-tumor-cells
#15
Aleksandr Klepinin, Lyudmila Ounpuu, Rita Guzun, Vladimir Chekulayev, Natalja Timohhina, Kersti Tepp, Igor Shevchuk, Uwe Schlattner, Tuuli Kaambre
The adenylate kinase (AK) isoforms network plays an important role in the intracellular energy transfer processes, the maintenance of energy homeostasis, and it is a major player in AMP metabolic signaling circuits in some highly-differentiated cells. For this purpose, a rapid and sensitive method was developed that enables to estimate directly and semi-quantitatively the distribution between cytosolic AK1 and mitochondrial AK2 localized in the intermembrane space, both in isolated cells and tissue samples (biopsy material)...
October 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27854029/acute-administration-of-3-5-diiodo-l-thyronine-to-hypothyroid-rats-stimulates-bioenergetic-parameters-in-liver-mitochondria
#16
Alessandro Cavallo, Federica Taurino, Fabrizio Damiano, Luisa Siculella, Anna Maria Sardanelli, Antonio Gnoni
The role of 3,5-diiodo-L-thyronine (T2), initially considered only a 3,3',5-triiodo-L-thyronine (T3) catabolite, in the bioenergetic metabolism is of growing interest. In this study we investigated the acute effects (within 1 h) of T2 administration to hypothyroid rats on liver mitochondria fatty acid uptake and β-oxidation rate, mitochondrial efficiency (by measuring proton leak) and mitochondrial oxidative damage (by determining H2O2 release). Fatty acid uptake into mitochondria was measured assaying carnitine palmitoyl transferase (CPT) I and II activities, and fatty acid β-oxidation using palmitoyl-CoA as a respiratory substrate...
October 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27816999/heterologous-expression-of-the-crassostrea-gigas-pacific-oyster-alternative-oxidase-in-the-yeast-saccharomyces-cerevisiae
#17
Aaron Robertson, Kyle Schaltz, Karina Neimanis, James F Staples, Allison E McDonald
Alternative oxidase (AOX) is a terminal oxidase within the inner mitochondrial membrane (IMM) present in many organisms where it functions in the electron transport system (ETS). AOX directly accepts electrons from ubiquinol and is therefore capable of bypassing ETS Complexes III and IV. The human genome does not contain a gene coding for AOX, so AOX expression has been suggested as a gene therapy for a range of human mitochondrial diseases caused by genetic mutations that render Complex III and/or IV dysfunctional...
October 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27796771/ii-insulin-processing-in-mitochondria
#18
María Del Carmen Camberos, Adriana A Pérez, Gisel A Passicot, Lucía C Martucci, María I Wanderley, Daniel P Udrisar, Juan C Cresto
Our objective was to know how insulin is processing in mitochondria; if IDE is the only participant in mitochondrial insulin degradation and the role of insulin degradation on IDE accumulation in mitoplasts. Mitochondria and its fractions were isolated as described by Greenwalt. IDE was purified and detected in immunoblot with specific antibodies. High insulin degradation was obtained through addition to rat's diet of 25 g/rat of apple and 10 g/rat of hard-boiled eggs, 3 days a week. Mitochondrial insulin degradation was assayed with 5 % TCA, insulin antibody or Sephadex G50 chromatography...
October 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27787743/respiratory-chain-inhibition-one-more-feature-to-propose-mptp-intoxication-as-a-leigh-syndrome-model
#19
Barbara Da Costa, Elodie Dumon, Laurence Le Moigno, Sylvie Bodard, Pierre Castelnau, Thierry Letellier, Christophe Rocher
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intoxicated mice have been widely used to model the loss of dopaminergic neurons. As this treatment leads to basal ganglia degeneration, it was proposed that MPTP mice could be used as a model of Leigh syndrome. However, this mitochondrial pathology is biochemically characterized by a respiratory chain dysfunction. To determine if MPTP can affect in vivo mitochondria function, we measured the activities of mitochondrial respiratory chain complexes in several tissues...
October 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27665468/mitochondrial-ca-2-uptake-pathways
#20
Pia A Elustondo, Matthew Nichols, George S Robertson, Evgeny V Pavlov
Calcium (Ca(2+)) plays diverse roles in all living organisms ranging from bacteria to humans. It is a structural element for bones, an essential mediator of excitation-contraction coupling, and a universal second messenger in the regulation of ion channel, enzyme and gene expression activities. In mitochondria, Ca(2+) is crucial for the control of energy production and cellular responses to metabolic stress. Ca(2+) uptake by the mitochondria occurs by the uniporter mechanism. The Mitochondrial Ca2+ Uniporter (MCU) protein has recently been identified as a core component responsible for mitochondrial Ca(2+) uptake...
September 24, 2016: Journal of Bioenergetics and Biomembranes
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