Julie Lerond, Bertrand Mathon, Mélina Scopin, Lucia Nichelli, Justine Guégan, Céline Bertholle, Brigitte Izac, Muriel Andrieu, Thomas Gareau, Florian Donneger, Badreddine Mohand Oumoussa, Franck Letourneur, Suzanne Tran, Mathilde Bertrand, Isabelle Le Roux, Mehdi Touat, Sophie Dupont, Jean Christophe Poncer, Vincent Navarro, Franck Bielle
OBJECTIVE: Mesial Temporal Lobe Epilepsy-associated Hippocampal Sclerosis (MTLE-HS) is a syndrome associated with various aetiologies. We previously identified CD34-positive extravascular stellate cells (CD34+ cells) possibly related to BRAFV600E oncogenic variant in a subset of MTLE-HS. We aimed to identify the BRAFV600E oncogenic variants and characterise the CD34+ cells. METHODS: We analysed BRAFV600E oncogenic variant by digital droplet Polymerase Chain Reaction in 53 MTLE-HS samples (25 with CD34+ cells) and 9 non-expansive neocortical lesions resected during epilepsy surgery (5 with CD34+ cells)...
September 23, 2023: Neuropathology and Applied Neurobiology