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Thrombosis and Haemostasis

Banne Nemeth, Raymond A van Adrichem, Astrid van Hylckama Vlieg, Trevor Baglin, Frits R Rosendaal, Rob G H H Nelissen, Saskia le Cessie, Suzanne C Cannegieter
Patients at high risk for venous thrombosis (VT) following knee arthroscopy could potentially benefit from thromboprophylaxis. We explored the predictive values of environmental, genetic risk factors and levels of coagulation markers to integrate these into a prediction model. Using a population-based case-control study into the aetiology of VT, we developed a Complete (all variables), Screening (easy to use in clinical practice) and Clinical (only environmental risk factors) model. The Clinical model was transformed into the Leiden-Thrombosis Risk Prediction (arthroscopy) score [ L-TRiP(ascopy) score ]...
September 20, 2018: Thrombosis and Haemostasis
Yan-Ping Geng, Di-Hui Lan, Nian Liu, Xin Du, Danni Zheng, Ri-Bo Tang, De-Yong Long, Rong-Hui Yu, Cai-Hua Sang, Rong Bai, Chen-Xi Jiang, Song-Nan Li, Xue-Yuan Guo, Wei Wang, Shi-Jun Xia, San-Shuai Chang, Jian-Zeng Dong, Ai-Hua Chen, Chang-Sheng Ma
BACKGROUND:  Anti-coagulant therapy satisfaction for patients with atrial fibrillation is a critical issue, which impacts on their treatment adherence and clinical outcomes. The disadvantages of long-term warfarin treatment are well-described, and novel oral anti-coagulants have become an alternative option. MATERIALS AND METHODS:  We compared patient-reported treatment satisfaction with dabigatran versus warfarin in non-valvular atrial fibrillation (NVAF) patients in China...
September 20, 2018: Thrombosis and Haemostasis
Valentina Biasin, Malgorzata Wygrecka, Thomas Bärnthaler, Katharina Jandl, Pritesh P Jain, Zoltán Bálint, Gabor Kovacs, Gerd Leitinger, Dagmar Kolb-Lenz, Karin Kornmueller, Florian Peters, Katharina Sinn, Walter Klepetko, Akos Heinemann, Andrea Olschewski, Christoph Becker-Pauly, Grazyna Kwapiszewska
Pulmonary arterial hypertension (PAH) is a rare disease characterized by increased pulmonary pressure and vascular remodelling as a consequence of smooth muscle cell proliferation, endothelial cell dysfunction and inflammatory infiltrates. Meprin α is a metalloproteinase whose substrates include adhesion and cell-cell contact molecules involved in the process of immune cell extravasation. In this study, we aimed to unravel the role of meprin α in PAH-induced vascular remodelling. Our results showed that meprin α was present in the apical membrane of endothelial cells in the lungs and pulmonary arteries of donors and idiopathic PAH (IPAH) patients...
September 20, 2018: Thrombosis and Haemostasis
Monica Sacco, Stefano Lancellotti, Federico Berruti, Alessandro Arcovito, Andrea Bellelli, Tiziana Ricciardelli, Ida Autiero, Luigi Cavallo, Romina Oliva, Raimondo De Cristofaro
The direct oral anticoagulant apixaban (APX), a strong factor Xa inhibitor, binds also to plasma proteins, especially albumin, and minimally to α1 -acid glycoprotein. Although APX can cross the red cell membrane, due to its chemical structure, and could bind to haemoglobin (Hb), no investigation was performed on this possible phenomenon that could affect the APX plasma concentration and thus its pharmacokinetics and pharmacodynamics. We addressed this issue by (1) measuring the levels of APX and haematological/biochemical parameters in 90 patients on APX therapy; (2) assessing the effect of APX on oxygen saturation curves of Hb; (3) testing the direct APX binding to Hb by fluorescence spectroscopy and a zinc-induced precipitation of Hb coupled to a reversed-phase high-performance liquid chromatography (HPLC)-based method; and (4) simulating in silico by molecular docking the APX interaction with human Hb...
September 20, 2018: Thrombosis and Haemostasis
Elien Roose, Gestur Vidarsson, Kadri Kangro, Onno J H M Verhagen, Ilaria Mancini, Linda Desender, Inge Pareyn, Nele Vandeputte, Aline Vandenbulcke, Chiara Vendramin, An-Sofie Schelpe, Jan Voorberg, Marie-Agnès Azerad, Laurent Gilardin, Marie Scully, Daan Dierickx, Hans Deckmyn, Simon F De Meyer, Flora Peyvandi, Karen Vanhoorelbeke
Immune-mediated thrombotic thrombocytopenic purpura (iTTP) is characterized by severe ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type 1 repeats, member 13) deficiency, the presence of anti-ADAMTS13 autoantibodies and an open ADAMTS13 conformation with a cryptic epitope in the spacer domain exposed. A detailed knowledge of anti-ADAMTS13 autoantibodies will help identifying pathogenic antibodies and elucidating the cause of ADAMTS13 deficiency. We aimed at cloning anti-ADAMTS13 autoantibodies from iTTP patients to study their epitopes and inhibitory characteristics...
September 20, 2018: Thrombosis and Haemostasis
Zorica Latinović, Adrijana Leonardi, Lidija Kovačič, Cho Yeow Koh, Jernej Šribar, Alenka Trampuš Bakija, Divi Venkateswarlu, R Manjunatha Kini, Igor Križaj
Components of the intrinsic blood coagulation pathway, among them factor VIIIa (FVIIIa), have been recognized as suitable therapeutic targets to treat venous thromboembolism, pathological process behind two very serious cardiovascular diseases, deep vein thrombosis and pulmonary embolism. Here, we describe a unique glycoprotein from the nose-horned viper ( Vipera ammodytes ammodytes [ Vaa ]) venom, Vaa serine proteinase homolog 1 ( Vaa SPH-1), structurally a serine protease but without an enzymatic activity and expressing potent anticoagulant action in human blood...
September 20, 2018: Thrombosis and Haemostasis
Sebastian G Hoß, Manuel Grundmann, Tobias Benkel, Lukas Gockel, Svenja Schwarz, Evi Kostenis, Martin Schlesinger, Neta Ilan, Israel Vlodavsky, Gerd Bendas
Heparanase (HPSE) is an endo-β-D-glucuronidase that cleaves heparan sulphate (HS) chains of proteoglycans (HSPGs). Besides a remodelling of the extracellular matrix, HPSE increases the bioavailability of pro-angiogenic mediators, such as HS-associated vascular endothelial growth factor (VEGF), thereby contributing to metastatic niche formation. Notably, HPSE also induces release of VEGF from tumour cells independent of its enzymatic activity, but the underlying molecular mechanisms remain unresolved. We found that exogenous addition of latent HPSE stimulates VEGF release from human MV3 melanoma cells...
September 20, 2018: Thrombosis and Haemostasis
Guosheng Li, Shuang Wang, Na Li, Yang Liu, Qi Feng, Xinyi Zuo, Xin Li, Yu Hou, Linlin Shao, Chunhong Ma, Chengjiang Gao, Ming Hou, Jun Peng
Primary immune thrombocytopaenia (ITP) is the most common haemorrhagic disease. Although most patients respond initially to mainstream therapies, such as corticosteroids, immunosuppressants or rituximab, a large proportion of patients fail to respond or relapse. These treatments only affect B lymphocytes or short-lived plasma cells, but not already existing long-lived plasma cells (LLPCs) which persistently secrete antibodies. We hypothesized that LLPCs may play a role in the corticosteroid-resistant or relapsed ITP patients, and bortezomib, a proteasome inhibitor, may act on plasma cells and offer a therapeutic effect...
September 20, 2018: Thrombosis and Haemostasis
Keunyoung Kim, Youn-Kyeong Chang, Yiying Bian, Ok-Nam Bae, Kyung-Min Lim, Jin-Ho Chung
BACKGROUND:  Paclitaxel is one of the most widely used anti-cancer drugs, but numerous case reports of thrombotic events in the cancer patients using paclitaxel raise concern over its pro-thrombotic risk. MATERIALS AND METHODS:  We investigated whether paclitaxel can elicit pro-thrombotic properties in red blood cells (RBCs) through phosphatidylserine (PS) exposure and microvesicle (MV) release. RESULTS:  In freshly isolated human RBCs, paclitaxel induced thrombin generation through PS exposure and MV release, whereas either coagulation factors or platelets were unaffected...
September 20, 2018: Thrombosis and Haemostasis
Tanja Falter, Stephanie Herold, Veronika Weyer-Elberich, Carina Scheiner, Veronique Schmitt, Charis von Auer, Xavier Messmer, Philipp Wild, Karl J Lackner, Bernhard Lämmle, Inge Scharrer
BACKGROUND:  Autoimmune thrombotic thrombocytopenic purpura (iTTP) is caused by autoantibody-mediated severe a disintegrin and metalloprotease with thrombospondin type 1 repeats, member 13 (ADAMTS13) deficiency leading to micro-angiopathic haemolytic anaemia (MAHA) and thrombocytopenia with organ damage. Patients survive with plasma exchange (PEX), fresh frozen plasma replacement and corticosteroid treatment. Anti-CD20 monoclonal antibody rituximab is increasingly used in patients resistant to conventional PEX or relapsing after an acute bout...
September 20, 2018: Thrombosis and Haemostasis
Wonhwa Lee, Seung-Yoon Park, Youngbum Yoo, Soon-Young Kim, Jung-Eun Kim, Shin-Woo Kim, Young-Kyo Seo, Eui Kyun Park, In-San Kim, Jong-Sup Bae
Sepsis develops because of overwhelming inflammatory responses to bacterial infection, and disrupts vascular integrity. Stabilin-1 (STAB-1) is a phagocytic receptor, which mediates efferocytosis in a phosphatidylserine (PS)-dependent manner. STAB-1 is expected to play important roles in efferocytosis during sepsis. Here, we determined the role of STAB-1 in maintaining and restoring vascular integrity. Macrophages and vascular endothelial cells were used to assess the effect of STAB-1 on survival rate, phagocytic activity, vascular permeability and transendothelial migration (TEM)...
September 20, 2018: Thrombosis and Haemostasis
Lisa Gross, David Jochheim, Tobias Nitschke, Moritz Baquet, Martin Orban, Lesca Holdt, Magda Zadrozny, Christian Hagl, Daniel Teupser, Axel Bauer, Steffen Massberg, Julinda Mehilli, Dirk Sibbing
Beyond thromboembolic events, peri-procedural bleeding remains one of the most frequent complications after transcatheter aortic valve implantation (TAVI). The majority of TAVI patients receive a dual anti-platelet treatment (DAPT) regimen. This analysis from the EVERY-TAVI register database aimed to analyse whether the level of on-treatment adenosine diphosphate-induced platelet reactivity predicts early outcomes at 30 days after TAVI. A total of 146 consecutive TAVI patients on DAPT who underwent platelet function testing with the Multiplate analyser were included here...
September 20, 2018: Thrombosis and Haemostasis
Laurent Bonello, Marc Laine, Gilles Lemesle, Etienne Puymirat, Thibaut Dabry, Franck Thuny, Franck Paganelli, Daniel Aradi, Corinne Frere, Stéphane Burtey, Dirk Sibbing, Julien Mancini
BACKGROUND:  The clinical benefit of anti-platelet agents in patients with chronic kidney disease (CKD) is uncertain. In addition, the risk-benefit ratio of potent oral P2Y12-adenosine diphosphate (ADP) receptor antagonists (PPAs), namely, prasugrel and ticagrelor, compared with clopidogrel in CKD patients suffering from acute coronary syndrome (ACS) remains unknown. OBJECTIVE:  We performed a meta-analysis of all studies comparing the clinical outcomes of PPA and clopidogrel therapy in CKD patients suffering from ACS...
September 20, 2018: Thrombosis and Haemostasis
Esther J Cooke, Jenny Y Zhou, Tine Wyseure, Shweta Joshi, Vikas Bhat, Donald L Durden, Laurent O Mosnier, Annette von Drygalski
No abstract text is available yet for this article.
August 2, 2018: Thrombosis and Haemostasis
James D Douketis, Alex C Spyropoulos, Julia M Anderson, Donald M Arnold, Shannon M Bates, Mark Blostein, Marc Carrier, Joseph A Caprini, Nathan P Clark, Michiel Coppens, Francesco Dentali, Joanne Duncan, Peter L Gross, Jeannine Kassis, Stephen Kowalski, Agnes Y Lee, Gregoire Le Gal, Geneviève Le Templier, Na Li, Elizabeth MacKay, Vinay Shah, Sudeep Shivakumar, Susan Solymoss, Frederick A Spencer, Summer Syed, Alfonso J Tafur, Thomas Vanassche, Thomas Thiele, Cynthia Wu, Erik Yeo, Sam Schulman
No abstract text is available yet for this article.
September 2018: Thrombosis and Haemostasis
Dominick J Angiolillo
No abstract text is available yet for this article.
September 2018: Thrombosis and Haemostasis
Markus Bender, Oliver Otto
No abstract text is available yet for this article.
September 2018: Thrombosis and Haemostasis
Hugo Maas, Savion Gropper, Fenglei Huang, Joachim Stangier, Igor Tartakovsky, Martina Brueckmann, Jacqueline M L Halton, Lesley G Mitchell
BACKGROUND:  Physiological age-related changes in the haemostatic and coagulation systems result in differing anticoagulant assay responses to standard anticoagulants. Therefore, we investigated the response of anticoagulant assays to dabigatran etexilate (DE) in children compared with adults. OBJECTIVE:  This article assesses the relationship between plasma dabigatran concentration and coagulation assay results across age groups in children and adults. PATIENTS AND METHODS:  Data from three clinical trials in which children received DE following standard of care for venous thromboembolism were compared with data from adult clinical trials...
September 2018: Thrombosis and Haemostasis
Kjersti Tunströmer, Lars Faxälv, Niklas Boknäs, Tomas L Lindahl
Imaging methods based on time-lapse microscopy are important tools for studying the dynamic events that shape thrombus formation upon vascular injury. However, there is a lack of methods to translate the vast amount of visual data generated in such experiments into quantitative variables describing platelet movements that can be subjected to systematic analysis. In this study, we developed experimental and computational protocols allowing for a detailed mathematical analysis of platelet movements within a developing thrombus...
September 2018: Thrombosis and Haemostasis
Janice Gomes, Fabrice Lucien, Tyler T Cooper, Yohan Kim, Karla C Williams, XinYang Liao, Lauren Kaufman, Francois Lagugné-Labarthet, Oliver Kenyon, Justin Boysen, Neil E Kay, Christopher W McIntyre, Hon S Leong
BACKGROUND:  Platelet microparticles (PMPs) and their abundance in the blood are a prognostic biomarker in thrombotic disorders and cancer. Nanoscale flow cytometry (nFC) is ideal for high-throughput analysis of PMPs but these clinical assays have not been developed previously. OBJECTIVE:  This article demonstrates that nFC is a suitable technology to enumerate PMPs present in plasma samples in a clinical setting. MATERIALS AND METHODS:  nFC was performed using the Apogee A50-Micro instrument...
September 2018: Thrombosis and Haemostasis
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