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Veterinary Parasitology

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https://www.readbyqxmd.com/read/28624132/schizogony-and-gametogony-of-oocyst-deficient-t-263-strain-of-toxoplasma-gondii
#1
J P Dubey
Oocysts are important stage for the spread of Toxoplasma gondii because they are environmentally resistant. Among all hosts of T. gondii, only felids can excrete oocysts. Cats that have excreted T. gondii oocysts after primary infection develop immunity to re-excretion of oocysts, and this immunity appears to be long-lasting. It would be desirable to have a non-infectious vaccine for the prevention of T. gondii infection in cats and to understand mechanism of immunity to excretion of oocysts. An initial step will be to indentify stage/stages of the parasite for induction of immunity...
May 27, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28592362/toxoplasma-gondii-apoptosis-and-cellular-division-alterations-after-in-vitro-treatment-with-copper-ii-complexes
#2
J A Portes, C S Motta, N F Azeredo, C Fernandes, A Horn, W De Souza, R A DaMatta, S H Seabra
Toxoplasma gondii is the causative agent of toxoplasmosis, which is one of the most common parasitic diseases in the world. Toxoplasmosis causes severe damage to immunocompromised hosts, and the most frequently used therapy is the combination of pyrimethamine and sulfadiazine, which has side effects. Thus, there is a need for new therapies that target T. gondii. Herein, we present the anti-Toxoplasma effect of two new copper(II) complexes: [(H2L1) Cu (μ-Cl)2 Cu(H2L1)] Cl2·5H2O (1) and [(H2L2) Cu (μ-Cl)2 Cu(H2L2)] Cl2·6H2O (2)...
April 20, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28413078/evaluation-of-immunochromatographic-test-ict-strips-for-the-serological-detection-of-babesia-bovis-and-babesia-bigemina-infection-in-cattle-from-western-java-indonesia
#3
Azirwan Guswanto, Puttik Allamanda, Euis Siti Mariamah, Tserendorf Munkjargal, Bumduuren Tuvshintulga, Hitoshi Takemae, Thillaiampalam Sivakumar, Mahmoud AbouLaila, Mohamad Alaa Terkawi, Madoka Ichikawa-Seki, Yoshifumi Nishikawa, Naoaki Yokoyama, Ikuo Igarashi
Three types of immunochromatographic test (ICT) strips were prepared for the detection of an antibody response against spherical body protein 4 (SBP-4) of Babesia bovis (bovICT), C-terminal-truncated rhoptry-associated protein 1 (rRAP1/CT17) of B. bigemina (bigICT), and the combination of both proteins (dual-ICT). The evaluation of their performance was conducted using a confirmed positive and negative serum panel for B. bovis and B. bigemina. Together with ELISA, the ICT strips were applied to determine the seroprevalence of bovine babesiosis in Western Java, Indonesia...
April 10, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28410950/detection-of-anaplasma-spp-and-ehrlichia-spp-anibodies-and-dirofilaria-immitis-antigens-in-dogs-from-seven-locations-of-morocco
#4
Sarah Elhamiani Khatat, Khalid Khallaayoune, Nabil Errafyk, Frans Van Gool, Luc Duchateau, Sylvie Daminet, Malika Kachani, Hamid El Amri, Rahma Azrib, Hamid Sahibi
In Morocco no data has been published on canine exposure to Anaplasma spp., Borrrelia burgdorferi, and Ehrlichia spp., and only one report is available on the occurrence of Dirofilaria immitis in dogs. Therefore, the aim of this study was to collect current data on the canine exposure to these vector-borne pathogens (VBPs) in Morocco. A total of 217 urban (n=57), rural (n=110) and military (n=50) dogs from seven Moroccan locations were screened for Anaplasma spp., B. burgdorferi and Ehrlichia spp. antibodies and for D...
April 4, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28408215/prevalence-genetic-diversity-and-potential-clinical-impact-of-blood-borne-and-enteric-protozoan-parasites-in-native-mammals-from-northern-australia
#5
Amanda Barbosa, Andrea Reiss, Bethany Jackson, Kristin Warren, Andrea Paparini, Graeme Gillespie, Danielle Stokeld, Peter Irwin, Una Ryan
A molecular survey was conducted to provide baseline information on the prevalence, genetic diversity and potential clinical impacts of blood-borne and enteric protozoans in native wild mammals from the Northern Territory (NT). A total of 209 blood and 167 faecal samples were collected from four target species; the northern brown bandicoot (Isoodon macrourus), common brushtail possum (Trichosurus vulpecula), northern quoll (Dasyurus hallucatus) and brush-tailed rabbit-rat (Conilurus penicillatus). Blood samples were screened by PCR at the 18S rRNA gene for trypanosomes, piroplasms and haemogregarines, with faecal samples tested for Cryptosporidium spp...
April 4, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28408214/prevalence-and-genotypic-characterization-of-bovine-echinococcus-granulosus-isolates-by-using-cytochrome-oxidase-1-co1-gene-in-hyderabad-pakistan
#6
Muhammad Ehsan, Nasreen Akhter, Bachal Bhutto, Abdullah Arijo, Javaid Ali Gadahi
Cystic echinococcosis is an important zoonotic disease; it has serious impacts on animals as well as human health throughout the world. Genotypic characterization of Echinocossus granulosus (E. granulosus) in buffaloes has not been addressed in Pakistan. Therefore, the present study was conducted to evaluate the incidence and genotypic characterization of bovine E. granulosus. Out of 832 buffaloes examined, 112 (13.46%) were found infected. The favorable site for hydatid cyst development was liver (8.65%) followed by lungs (4...
April 4, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28413077/trichomonosis-outbreak-in-a-flock-of-canaries-serinus-canaria-f-domestica-caused-by-a-finch-epidemic-strain-of-trichomonas-gallinae
#7
Marko Zadravec, Brigita Slavec, Uroš Krapež, Mitja Gombač, Tanja Švara, Mateja Poljšak-Prijatelj, Igor Gruntar, Joško Račnik
In the present paper, an outbreak of trichomonosis in a flock of 15 breeding pairs of canaries is described. Trichomonosis was diagnosed on characteristic clinical signs, microscopic examination of crop/esophageal swabs, gross pathology and histopathology. Trichomonads were successfully grown in culture media and were characterized by multi-locus sequence typing. The three genomic loci ITS1-5.8S-ITS2, 18S rRNA and Fe-hydrogenase were analyzed. Molecular characterization confirmed the finch trichomonosis strain, identical to the strain that caused emerging disease in free-living passerine birds in Europe...
April 3, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28404209/investigation-of-toxoplasma-gondii-in-semen-testicle-and-epididymis-tissues-of-primo-infected-cats-felis-catus
#8
Weslen Fabricio Pires Teixeira, Maria Eduarda Gonçalves Tozato, Julia Cestari Pierucci, Giovana Pavão Vital, André Cayeiro Cruz, Welber Daniel Zanetti Lopes, Marina Suzuki Cursino, Samea Fernandes Joaquim, Vando Edésio Soares, Helio Langoni, Kátia Denise Saraiva Bresciani, Alvimar José da Costa
This study aimed to investigate the presence of Toxoplasma gondii in semen, testicle and epididymis tissues of cats experimentally infected by this coccidium. A total of 12 male felines without a definite breed that were of reproductive age and serologically negative for T. gondii were selected and distributed to the following three experimental groups: GI, inoculated with 600 tissue cysts of the P strain of T. gondii (isolate III); GII, inoculated with 2×10(5) tachyzoites of the RH strain (isolate I); and GIII, not inoculated (control group)...
April 3, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28408213/efficacy-of-afoxolaner-plus-milbemycin-oxime-chewable-tablets-nexgard-spectra-%C3%A2-merial-against-adult-ancylostoma-ceylanicum-hookworm-in-dogs
#9
E Tielemans, W Lebon, P Dumont, P Taweethavonsawat, D Larsen, S Rehbein
A fixed-combination chewable tablet incorporating afoxolaner plus milbemycin oxime (NexGard Spectra(®), Merial) was tested in purpose-bred Beagle dogs for efficacy against adult Ancylostoma ceylanicum hookworms. Sixteen dogs were inoculated each by oral administration of approximately 500 infective larvae of A. ceylanicum. Seventeen days after inoculation, the dogs were weighed and allocated randomly to be treated with afoxolaner plus milbemycin oxime chewable tablets or to remain untreated. Commercial chewable tablets of different strength were combined to deliver doses as close as possible to the minimum effective dose of 2...
April 1, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28385540/performance-of-leishmania-braziliensis-enolase-protein-for-the-serodiagnosis-of-canine-and-human-visceral-leishmaniosis
#10
Mariana Costa Duarte, Daniela Pagliara Lage, Vívian Tamietti Martins, Lourena Emanuele Costa, Beatriz Cristina Silveira Salles, Ana Maria Ravena Severino Carvalho, Thaís Teodoro de Oliveira Santos, Daniel Silva Dias, Patrícia Aparecida Fernandes Ribeiro, Miguel Angel Chávez-Fumagalli, Ricardo Andrez Machado-de-Ávila, Bruno Mendes Roatt, Daniel Menezes-Souza, Danielle Ferreira de Magalhães-Soares, Eduardo Antonio Ferraz Coelho
In the present study, Leishmania braziliensis enolase was cloned and the recombinant protein (rEnolase) was evaluated for the serodiagnosis of canine and human visceral leishmaniosis (VL). For the canine VL diagnosis, this study examined serum samples of Leishmania infantum-infected dogs, from non-infected animals living in endemic or non-endemic areas of leishmaniosis, as well as those from Leish-Tec(®)-vaccinated dogs and Trypanosoma cruzi or Ehrlichia canis experimentally infected animals. For the human VL diagnosis, this study analyzed serum samples from VL patients, from non-infected subjects living in endemic or non-endemic areas of leishmaniosis, as well as those from T...
March 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28385541/plant-products-and-secondary-metabolites-with-acaricide-activity-against-ticks
#11
REVIEW
J A Rosado-Aguilar, K Arjona-Cambranes, J F J Torres-Acosta, R I Rodríguez-Vivas, M E Bolio-González, A Ortega-Pacheco, A Alzina-López, E J Gutiérrez-Ruiz, E Gutiérrez-Blanco, A J Aguilar-Caballero
The present review documents the results of studies evaluating the acaricidal activity of different plant products and secondary metabolites against ticks that are resistant and susceptible to conventional acaricides. Studies published from 1998 to 2016 were included. The acaricidal activity of plant extracts, essential oils and secondary compounds from plants have been evaluated using bioassays with ticks in the larval and adult stages. There is variable effectiveness according to the species of plant and the concentrations used, with observed mortalities ranging from 5 to 100% against the Rhipicephalus (Boophilus), Amblyomma, Dermacentor, Hyalomma, and Argas genera...
March 27, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28377149/evaluation-of-an-association-of-alpha-cypermethrin-and-flufenoxuron-for-tick-control-in-an-area-at-risk-of-brazilian-spotted-fever
#12
José Brites-Neto, Jardel Brasil, Jeferson de Andrade, Gabriele Luciana Saqui
Brazilian spotted fever (BSF) is an endemic anthropozoonosis caused by Gram-negative bacteria (Rickettsia rickettsii) that is associated with the risk of human infection by ticks. In the city of Americana, São Paulo (SP), Brazil, there were 12 cases (67% lethal) between 2004 and 2015. This study evaluated the effectiveness of control over tick infestations in an area at risk of BSF, in a field trial on an association of alpha-cypermethrin (3%) and flufenoxuron (3%) (Tenopa(®)) conducted in Americana. The infestation levels of the target species (Amblyomma sculptum and Amblyomma dubitatum) in the field trial were evaluated through acarological surveys (CO2 traps) on days 0, 22, 43 and 62 after treatment, both in the treatment area and in a control area...
March 27, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28392045/low-prevalence-of-zoonotic-babesia-in-small-mammals-and-ixodes-ricinus-in-brittany-france
#13
M Jouglin, G Perez, A Butet, L Malandrin, S Bastian
In order to evaluate the zoonotic risk due to Babesia spp., especially B. microti, we investigated their presence in 597 individuals of five small mammal species and in 2620 questing nymphs of Ixodes ricinus in rural landscapes of Western France (Brittany). Small mammals (rodents and shrews) are indeed suspected to be reservoir hosts for B. microti, and the tick I. ricinus is the vector of the three main zoonotic species in Europe, i.e. B. divergens, B. venatorum and B. microti. Only one bank vole carried B...
March 25, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28385539/th1-th2-balance-in-the-liver-and-hepatic-lymph-nodes-of-vaccinated-and-unvaccinated-sheep-during-acute-stages-of-infection-with-fasciola-hepatica
#14
I L Pacheco, N Abril, N Morales-Prieto, M J Bautista, R Zafra, A Escamilla, M T Ruiz, A Martínez-Moreno, J Pérez
The expression of IFNγ and IL4 was quantified using q-PCR in the liver and hepatic lymph nodes (HLN) of sheep during early stages of infection with Fasciola hepatica (1, 3, 9 and 18days post-infection, dpi). A group of animals (Group 1) were vaccinated with Fasciola hepatica recombinant cathepsin L1 (FhCL1) in montanide 70 VG prior to infection, a second group (group 2) was used as infected control and a third (group 3) was used as uninfected control. To study vaccine efficacy three additional groups were sacrificed 19 weeks post-infection (group 4 immunized with CL1, group 5 with the adjuvant and group 6 was used as infected control)...
March 25, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28606328/influence-of-physical-and-chemical-factors-on-the-embryonation-hatching-and-infectivity-of-spirocerca-lupi
#15
Alicia Rojas, Niva Freedberg, Alex Markovics, Yuval Gottlieb, Gad Baneth
Spirocerca lupi is a parasitic nematode which causes spirocercosis, a severe disease of dogs. Its life cycle involves dung beetles as intermediate hosts and canids as definitive hosts. The effect of different physical conditions and chemical factors on the embryonation and hatching of S. lupi eggs were investigated in this study in order to understand the triggers for progression in the early development of this parasite. Exposure to potassium dichromate significantly enhanced the embryonation of eggs compared to formaldehyde and controls (p<0...
August 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28606327/evaluation-of-oxidant-antioxidant-balance-in-iberian-ibex-capra-pyrenaica-experimentally-infested-with-sarcoptes-scabiei
#16
José Espinosa, Jesús M Pérez, Jorge R López-Olvera, Arián Ráez-Bravo, Francisco J Cano-Manuel, Paulino Fandos, Ramón C Soriguer, José Enrique Granados, Diego Romero
Oxidative stress (OS) is an imbalance between radical-generating and radical scavenging activity, resulting in oxidation products and tissue damage. Although some studies have been done in other species, there is a lack of information about the oxidative/antioxidant status in the Iberian ibex (Capra pyrenaica) affected by sarcoptic mange. To clarify this fact, albumin, catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), paraoxonase-1 (PON-1), glutathione reductase (GR), reduced glutathione (GSH): oxidized glutathione (GSSG) ratio, thiobarbituric acid reactive substances (TBARS) and total oxidant status (TOS) concentrations were measured in peripheral blood of ibexes experimentally infested with Sarcoptes scabiei (n=25), as well as in the healthy control group (n=14)...
August 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28606326/evaluation-of-the-transcription-of-interleukin-12-in-the-intestinal-mucosa-of-mice-subjected-to-experimental-toxocariasis-and-supplemented-with-saccharomyces-boulardii
#17
Micaele Quintana de Moura, Wesley Douglas da Silva Terto, Sabrina Taiza Jeske, Leonardo Mortagua de Castro, Natália Berne Pinto, Luciana Farias da Costa Avila, Fabio Pereira Leivas Leite, Maria Elisabeth Aires Berne
No abstract text is available yet for this article.
August 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28606325/quantification-of-anti-leishmania-antibodies-in-saliva-of-dogs
#18
Ana Cantos-Barreda, Damián Escribano, Luis J Bernal, José J Cerón, Silvia Martínez-Subiela
Detection of serum anti-Leishmania antibodies by quantitative or qualitative techniques has been the most used method to diagnose Canine Leishmaniosis (CanL). Nevertheless, saliva may represent an alternative to blood because it is easy to collect, painless and non-invasive in comparison with serum. In this study, two time-resolved immunofluorometric assays (TR-IFMAs) for quantification of anti-Leishmania IgG2 and IgA antibodies in saliva were developed and validated and their ability to distinguish Leishmania-seronegative from seropositive dogs was evaluated...
August 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28606324/serological-lessons-from-the-bovine-lungworm-dictyocaulus-viviparus-antibody-titre-development-is-independent-of-the-infection-dose-and-reinfection-shortens-seropositivity
#19
Christina Strube, Andrea Springer, Anne-Marie Schunn, Andrew B Forbes
Infections with the bovine lungworm Dictyocaulus viviparus, the causative agent of parasitic bronchitis, are accompanied by substantial economic losses due to impacts on production, clinical respiratory disease or even death of diseased cattle. To detect lungworm antibodies in cattle, an enzyme-linked immunosorbent assay (ELISA) based on recombinant major sperm protein (MSP) has been developed. However, it remained unknown whether the infection dose influences antibody levels, and how acquired immunity influences antibody level patterns during reinfections...
August 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28606323/coprodiagnosis-of-echinococcus-granulosus-infection-in-dogs-from-ankara-turkey
#20
Hatice Öge, Semih Öge, Bahadır Gönenç, Oğuz Sarımehmetoğlu, Gökben Özbakış
The present study were undertaken to compare two isolation techniques (centrifugal flotation and sedimentation) for recovering taeniid eggs from faecal samples, to identify E. granulosus DNA from taeniid eggs by PCR, and to determine the prevalence of E. granulosus in dogs in villages. Faecal samples were collected from 100 dogs in Ankara province. Taenia spp. eggs were found in 27% of dogs faeces. Echinococcus granulosus-specific PCR was obtained in 14 (51.85%) of the taeniid eggs-positive samples. As well as finding Taenia eggs in dogs' faeces, we also found eggs of some helminthic parasites; such as Dipylidium caninum, Toxocara canis, Toxascaris leonina, Trichuris sp...
August 15, 2017: Veterinary Parasitology
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