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Veterinary Parasitology

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https://www.readbyqxmd.com/read/28734669/demodex-mites-in-lymph-nodes
#1
LETTER
Melissa A Nashat
No abstract text is available yet for this article.
July 13, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28935118/ex-vivo-trypanocidal-activity-of-1-2-hydroxybenzylidene-thiosemicarbazide-against-trypanosoma-equiperdum
#2
N Parra, M Jaume, K Boscán, A Hernández, A Mijares, M González, Y Alvarado, J Restrepo
Trypanosoma equiperdum is the causative agent of dourine, a venereal disease in horses and donkeys. This parasite has a widely distribution, is found in Africa, Asia, Southern and Eastern Europe, Russia, Mexico and Venezuela. The T. equiperdum is morphologically indistinguishable to other Trypanozoon species, however differs from other mammalian trypanosomes due to the fact that it is primarily a tissue parasite, generating cutaneous plaques, swelling of genitalia and neurological signs. The aim of this study was to evaluate the trypanocidal effectiveness of a set of derivatives of thiosemicarbazones on a T...
July 8, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917326/serum-levels-of-cytokines-in-water-buffaloes-experimentally-infected-with-fasciola-gigantica
#3
Fu-Kai Zhang, Ai-Jiang Guo, Jun-Ling Hou, Miao-Miao Sun, Zhao-An Sheng, Xiao-Xuan Zhang, Wei-Yi Huang, Hany M Elsheikha, Xing-Quan Zhu
Fasciola gigantica infection in water buffaloes causes significant economic losses especially in developing countries. Although modulation of the host immune response by cytokine neutralization or vaccination is a promising approach to control infection with this parasite, our understanding of cytokine's dynamic during F. gigantica infection is limited. To address this, we quantified the levels of serum cytokines produced in water buffaloes following experimental infection with F. gigantica. Five buffaloes were infected via oral gavage with 500 viable F...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917325/comparison-of-five-diagnostic-tests-for-giardia-duodenalis-in-fecal-samples-from-young-dogs
#4
Fabienne D Uehlinger, S Ali Naqvi, Spencer J Greenwood, J Trenton McClure, Gary Conboy, Ryan O'Handley, Herman W Barkema
Five diagnostic tests were compared for the diagnosis of Giardia duodenalis in fecal samples of young dogs. Fecal samples were collected from 136 healthy dogs <1year old and examined using immunofluorescence antibody microscopy (IFA) after sucrose gradient centrifugation, zinc sulfate centrifugal flotation technique (ZSCT), SNAP(®)Giardia test, and ProSpecT(®)Giardia EZ Microplate assay. In addition, polymerase chain reaction (PCR) of the 16S rRNA gene was performed. Kappa (κ) statistic was calculated to assess diagnostic agreement between the IFA and each test...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917324/targeted-anthelmintic-treatment-of-parasitic-gastroenteritis-in-first-grazing-season-dairy-calves-using-daily-live-weight-gain-as-an-indicator
#5
A Jackson, K A Ellis, J McGoldrick, N N Jonsson, M J Stear, A B Forbes
Control of parasitic gastroenteritis in cattle is typically based on group treatments with anthelmintics, complemented by grazing management, where feasible. However, the almost inevitable evolution of resistance in parasitic nematodes to anthelmintics over time necessitates a reappraisal of their use in order to reduce selection pressure. One such approach is targeted selective treatment (TST), in which only individual animals that will most benefit are treated, rather than whole groups of at-risk cattle. This study was designed to assess the feasibility of implementing TST on three commercial farms, two of which were organic...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917323/seroprevalence-and-first-multilocus-microsatellite-genotyping-of-neospora-caninum-in-dairy-cattle-in-henan-central-china
#6
Weifeng Qian, Tianqi Wang, Wenchao Yan, Min Zhang, Lifang Han, Rui Xue, Shaofu Song, Chaochao Lv
Neospora caninum is one of the important causes of abortion in cattle worldwide, and losses due to neosporosis to the cattle industry are considerable. However, the knowledge of genetic characterization of this parasite is limited. The aim of the present study is to determine the prevalence and genetic characterization of N. caninum from dairy cows in Henan Province, central China. A total of 510 blood samples and 7 aborted fetuses were collected from 8 dairy farms in Henan Province. Serum antibodies to N. caninum were examined by ELISA using a recombinant tNcSRS2 protein as the coating antigen...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917322/high-genetic-diversity-in-toxoplasma-gondii-isolates-from-pigs-at-slaughterhouses-in-para%C3%A3-ba-state-northeastern-brazil-circulation-of-new-genotypes-and-brazilian-clonal-lineages
#7
Thais Ferreira Feitosa, Vinícius Longo Ribeiro Vilela, João Leite de Almeida-Neto, Antonielson Dos Santos, Dayana Firmino de Morais, Bruna Farias Alves, Fabiana Nakashima, Solange Maria Gennari, Ana Célia Rodrigues Athayde, Hilda Fátima Jesus Pena
The consumption of raw or undercooked pig meat containing Toxoplasma gondii cysts is an important transmission route of this protozoon to animals and humans. This study aimed to serologically diagnose, isolate and genotype T. gondii from pigs slaughtered for human consumption in the state of Paraíba, northeastern Brazil. Blood and tissue samples (heart, tongue and brain) were collected from 120 pigs at slaughterhouses in the state of Paraíba. Serological examinations were performed with an indirect immunofluorescent antibody test (IFAT) with a cut-off point of 1:64...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917321/a-panel-of-microsatellite-markers-to-discriminate-and-study-interactions-between-haemonchus-contortus-and-haemonchus-placei
#8
Michelle C Santos, Elizabeth Redman, Mônica R V Amarante, John S Gilleard, Alessandro F T Amarante
Haemonchus contortus and Haemonchus placei are two closely related economically important parasites of ruminants. Their close morphological similarity, common occurrence as co-infections and ability to hybridize makes definitive diagnosis and epidemiological studies in field populations challenging. In this paper, we describe the development of a panel of microsatellite markers that can be used to discriminate and study the genetics of these two parasite species in co-infections and mixed field populations...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917320/vector-borne-parasitic-infections-in-dogs-in-the-baltic-and-nordic-countries-a-questionnaire-study-to-veterinarians-on-canine-babesiosis-and-infections-with-dirofilaria-immitis-and-dirofilaria-repens
#9
Valentina Tiškina, Pikka Jokelainen
Canine vector-borne diseases have been spreading northwards in Europe, and canine babesiosis and infections with Dirofilaria immitis (heartworm) and Dirofilaria repens have been diagnosed also in the Baltic and the Nordic countries. We used an online questionnaire to survey how large a proportion of veterinarians in the Baltic (Estonia, Latvia, and Lithuania) and the Nordic countries (Denmark, Finland, Iceland, Norway, and Sweden) saw canine babesiosis cases and dogs infected with D. immitis and D. repens in 2016...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917319/morphological-and-molecular-analyses-of-tylodelphys-spp-metacercaria-trematoda-diplostomidae-from-the-vitreous-humour-of-two-freshwater-fish-species-channa-gachua-ham-and-puntius-sophore-ham
#10
Anshu Chaudhary, Shivi Gupta, Richa Tripathi, Hridaya S Singh
Trematodes of family Diplostomatidae consists of a large and diverse group of parasites which have larval stages that are very important pathogens of wild and cultured freshwater fishes worldwide, can cause serious impacts. Our understanding of the diplostomids diversity, though, remains deficient and limited especially in India. In the present study, the morphology and molecular characterization of Tylodelphys spp. from the vitreous humour of eye, collected from two economically important food fish, dwarf snakehead Channa gachua (Perciformes: Channidae) and pool barb Puntius sophore (Cypriniformes: Cyprinidae) were described from Bijnor and Meerut, Uttar Pradesh (U...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917318/diversity-of-spirocerca-lupi-in-domestic-dogs-and-black-backed-jackals-canis-mesomelas-from-south-africa
#11
Wiekolize Rothmann, Pamela J de Waal
Spirocerca lupi is a parasitic nematode that causes spirocercosis predominantly in domestic dogs. Spirocerca lupi nematode samples were collected from four regions around South Africa and analyzed to compare the genetic diversity among the regions. A total of 56 S. lupi nematodes were obtained by necropsy from domestic dogs and wild black-backed jackals (Canis mesomelas). Sixteen different haplotypes of cox1 were identified some of which are shared between regions as well as with black-backed jackal. The genetic similarity between S...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917317/hammondia-heydorni-oocyst-shedding-by-dogs-fed-in-vitro-generated-tissue-cysts-and-evaluation-of-cross-immunity-between-h-heydorni-and-neospora-caninum-in-mice
#12
I D S Meneses, G Schares, M M Rezende-Gondim, G S Galvão, L F P Gondim
Hammondia heydorni is a coccidian parasite believed to be nonpathogenic for naturally-infected animals, but it is biologically and genetically related to Neospora caninum, a worldwide cause of abortion in cattle. The major aim of the present work was to determine whether dogs shed H. heydorni oocysts after consuming in vitro generated tissue cysts of the parasite. In addition, we investigated cross-immunity between H. heydorni and N. caninum in mice. Two dogs were fed cultured cells containing tissue cysts of H...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917316/humoral-and-cytokine-response-elicited-during-immunisation-with-recombinant-immune-mapped-protein-1-etimp-1-and-oocysts-of-eimeria-tenella
#13
Krishnendu Kundu, Rajat Garg, Saroj Kumar, Mrityunjay Mandal, Fiona M Tomley, Damer P Blake, Partha Sarathi Banerjee
Eimeria tenella, the causative agent of caecal coccidiosis, is a pathogenic gut dwelling protozoan which can cause severe morbidity and mortality in farmed chickens. Immune mapped protein-1 (IMP-1) has been identified as an anticoccidial vaccine candidate; in the present study allelic polymorphism was assessed across the IMP-1 coding sequence in E. tenella isolates from four countries and compared with the UK reference Houghton strain. Nucleotide diversity was low, limited to expansion/contraction of a CAG triplet repeat and five substitutions, three of which were non-synonymous...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917315/protective-and-risk-factors-associated-with-the-presence-of-toxocara-spp-eggs-in-dog-hair
#14
Yslla Fernanda Fitz Balo Merigueti, Vamilton Alvares Santarém, Lívia Magosso Ramires, Aline da Silveira Batista, Layron Vinícus da Costa Beserra, Amábyle Lopes Nuci, Talita Mirella de Paula Esposte
Toxocariasis is one of the most prevalent parasitic zoonoses in the world. The disease is principally caused by the nematode Toxocara canis, whose definitive host is the dog. The transmission of toxocariasis to humans is mainly caused by accidental ingestion of embryonated eggs of the parasite, present in the soil. Studies have shown that dog hair has the capacity to harbor eggs of the parasite and represents a risk for transmission of the zoonosis. The objective of the present study was to evaluate the frequency and factors associated with the contamination of dog hair by Toxocara spp...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917314/evaluation-of-cardiopulmonary-and-inflammatory-markers-in-dogs-with-heartworm-infection-treated-using-the-slow-kill-method
#15
W-K Yoon, Y-W Kim, S-I Suh, C Hyun
This study evaluated the changes in the levels of cardiac, hemostatic, and inflammatory biomarkers in 12 dogs with different severities of heartworm infection treated using the slow kill protocol, consisting of 6-10μg/kg of ivermectin and 10mg/kg of doxycycline combination. The serum levels of cardiac troponin-I, D-dimer, C-reactive protein, and interleukin-6 were measured on the day of diagnosis (D0), after termination of doxycycline administration (D30), after termination of the slow kill treatment (D180), and 10 months after the initiation of therapy (D300)...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917313/field-evaluation-of-poultry-red-mite-dermanyssus-gallinae-native-and-recombinant-prototype-vaccines
#16
Kathryn Bartley, Frank Turnbull, Harry W Wright, John F Huntley, Javier Palarea-Albaladejo, Mintu Nath, Alasdair J Nisbet
Vaccination is a desirable emerging strategy to combat poultry red mite (PRM), Dermanyssus gallinae. We performed trials, in laying hens in a commercial-style cage facility, to test the vaccine efficacy of a native preparation of soluble mite extract (SME) and of a recombinant antigen cocktail vaccine containing bacterially-expressed versions of the immunogenic SME proteins Deg-SRP-1, Deg-VIT-1 and Deg-PUF-1. Hens (n=384 per group) were injected with either vaccine or adjuvant only (control group) at 12 and 17 weeks of age and then challenged with PRM 10days later...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917312/first-finding-of-nymphal-stages-of-linguatula-serrata-in-a-south-american-camelid-a-vicu%C3%A3-a-from-peru
#17
Luis A Gomez-Puerta, Joel I Pacheco, José M Angulo-Tisoc, Maria T Lopez-Urbina, Armando E Gonzalez
Linguatula serrata, a pentastomid, was found parasitizing the lungs of a vicuña (Vicugna vicugna) from Cuzco, Peru. A total of 13 larvae were found encysted in the parenchymal tissue of the lungs. All larvae were identified as nymphal stages of L. serrata by morphological methods Diagnosis was confirmed by molecular analysis amplifying the cytochrome c oxidase 1 gene of three nymphs. Nucleotide sequences from the isolates were compared to previous sequences from GenBank, and it showed high similarity between them (>99%)...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917311/a-new-lyophilized-tachyzoite-based-elisa-to-diagnose-besnoitia-spp-infection-in-bovids-and-wild-ruminants-improves-specificity
#18
P García-Lunar, L M Ortega-Mora, G Schares, C Diezma-Díaz, G Álvarez-García
Recent studies have reported that routinely used whole or soluble Besnoitia besnoiti tachyzoite (TZ) extract-based ELISAs potentially give rise to a high number of false-positive results, which may compromise control and the epidemiological studies of bovine besnoitiosis. Thus, western blot (WB) has been recommended as a confirmatory test. In the present study, a new ELISA test that employs lyophilized tachyzoites for the first time (BbSALUVET ELISA 2.0) was developed and validated with cattle sera (n=606) under a worst-case scenario...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917310/screening-of-a-small-well-curated-natural-product-based-library-identifies-two-rotenoids-with-potent-nematocidal-activity-against-haemonchus-contortus
#19
H M P Dilrukshi Herath, Sarah Preston, Andreas Hofmann, Rohan A Davis, Anson V Koehler, Bill C H Chang, Abdul Jabbar, Robin B Gasser
The control of parasitic roundworms (nematodes) is heavily reliant on the use of a limited number of anthelmintic drugs. However, drug resistance is now very widespread and no vaccines are available, such that the discovery of new chemical entities is crucial. Within this context, we screened a library of pure natural products (n=400) against exsheathed third-stage (xL3) larvae of the parasitic nematode Haemonchus contortus using a whole-organism screening method. We identified two plant-derived rotenoids, deguelin and rotenone, with inhibitory activity on xL3 motility...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917309/toxic-action-of-acmella-oleracea-extract-on-the-male-reproductive-system-of-amblyomma-cajennense-ticks
#20
Luís Adriano Anholeto, Patrícia Rosa de Oliveira, Rodney Alexandre Ferreira Rodrigues, Lais Thiemi Yamane, Karina Neoob de Carvalho Castro, Allan Roberto Fernandes Ferreira, Maria Izabel Camargo-Mathias
The present study evaluated through morphohistological and histochemical techniques the effects of different concentrations of crude ethanolic extract of A. oleracea (EEAO) (Jambu) on the male reproductive system of Amblyomma cajennense sensu stricto (s.s.) ticks. The toxicity of this natural chemical was stablished, signalizing the promising potential of the compound as a strategy to control ectoparasites in the near future. For the experiment, 100 males fed on host rabbits with homogeneous weight (p>0...
September 15, 2017: Veterinary Parasitology
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