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Journal of Endodontics

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https://www.readbyqxmd.com/read/27871783/human-dental-pulp-stem-cells-suppress-alloantigen-induced-immunity-by-stimulating-t-cells-to-release-transforming-growth-factor-beta
#1
Kyu Hwan Kwack, Jung Min Lee, Sang Hyuk Park, Hyeon Woo Lee
INTRODUCTION: Human dental pulp stem cells (hDPSCs) are ideal candidates for regenerating damaged dental tissue. To examine the possibility that hDPSCs may be used to regenerate pulp, we tested their in vitro effects on acute allogeneic immune responses. METHODS: A peripheral blood mononuclear cell (PBMC) proliferation assay and immunoglobulin (Ig) production assay were performed to evaluate the immunosuppressive properties of hDPSCs. RESULTS: The mixed lymphocyte reaction was suppressed by incubation with hDPSCs...
November 18, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27866710/pulpal-tissue-inflammatory-reactions-after-experimental-pulpal-exposure-in-mice
#2
Ying He, Youhua Gan, Jiajian Lu, Qiong Feng, Haiyan Wang, Hongbing Guan, Qianzhou Jiang
INTRODUCTION: The purpose of this study was to establish a stable experimental mice pulpal inflammatory model and to evaluate inflammatory reactions of pulpal tissue after pulpal exposure. METHODS: Pulpal inflammation was induced in 80 C57BL/6 mice by occlusal exposure of the pulp of the maxillary first molar. The mice were sacrificed randomly at 0, 1, 6, 12, 24, 48, and 72 hours after pulpal exposure. Mice without pulpal exposure served as controls. Maxillary teeth were obtained and prepared for histologic analyses and real-time polymerase chain reaction analyses...
November 17, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27847139/stem-cell-marker-expression-in-persistent-apical-periodontitis
#3
Carlos Estrela, Brunno Santos Freitas Silva, Júlio A Silva, Fernanda P Yamamoto-Silva, Décio Dos Santos Pinto-Júnior, Ricardo Santiago Gomez
INTRODUCTION: This study evaluated the expression of CD90 (mesenchymal stem cell) and Sox2 (progenitor stem cell) markers in persistent apical periodontitis (PAP) (n = 16) and primary periapical lesions (PPLs) (n = 10). METHODS: All samples were classified histologically according to the intensity of inflammatory cell infiltrate in the periapical lesion. Immunohistochemistry was used to detect CD90 and Sox2 in PAP and PPLs. The Spearman correlation coefficient and the Mann-Whitney U test were used to analyze data at the 5% significance level...
November 12, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27847138/a-comparative-evaluation-of-the-effect-of-the-addition-of-pachymic-acid-on-the-cytotoxicity-of-4-different-root-canal-sealers-an-in%C3%A2-vitro-study
#4
Senthamilselvan Arun, Vidhya Sampath, Sekar Mahalaxmi, Kothandaraman Rajkumar
INTRODUCTION: Root canal sealers exhibit varying degrees of cytotoxicity to periapical tissues. This in turn results in inflammation, delayed wound healing, and even bone resorption. This study aimed to explore the effect of the addition of an antioxidant like pachymic acid on the cytotoxicity of 4 root canal sealers, namely, Tubliseal (Kerr, Romulus, MI), a zinc oxide eugenol-based sealer; AH Plus (Dentsply De Trey GmbH, Konstanz, Germany), an epoxy resin-based sealer; Sealapex (Kerr), a calcium hydroxide-based sealer; and EndoREZ (Ultradent Products, South Jordan, UT), a methacrylate resin-based sealer...
November 12, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27847137/essential-roles-of-bone-morphogenetic-protein-1-and-mammalian-tolloid-like-1-in-postnatal-root-dentin-formation
#5
Jun Wang, Alison M Muir, Yinshi Ren, Dawiyat Massoudi, Daniel S Greenspan, Jian Q Feng
INTRODUCTION: Mutations in the proteinase bone morphogenetic protein-1 (BMP1) were recently identified in patients with osteogenesis imperfecta, which can be associated with type 1 dentinogenesis imperfecta. BMP1 is co-expressed in various tissues and has overlapping activities with the closely related proteinase mammalian tolloid-like 1 (TLL1). In this study we investigated whether removing the overlapping activities of BMP1 and TLL1 affects the mineralization of tooth root dentin. METHODS: Floxed alleles of the BMP1 and TLL1 genes were excised via ubiquitously expressed Cre induced by tamoxifen treatment beginning at 3 days of age (harvested at 3 weeks of age) or beginning at 4 weeks of age (harvested at 8 weeks of age)...
November 12, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27847136/evaluation-of-two-trephine-techniques-for-removal-of-fractured-rotary-nickel-titanium-instruments-from-root-canals
#6
Qian Yang, Ya Shen, Dingming Huang, Xuedong Zhou, Yuan Gao, Markus Haapasalo
INTRODUCTION: The aim of this in vitro study was to evaluate the effects on root dentin of 2 trephining techniques using an ultrasonic tip or a trepan bur in the mesial canals of mandibular molars during attempts to remove fractured file fragments using micro-computed tomographic imaging. METHODS: Twenty-one teeth with a similar anatomic configuration in mesial (buccal and lingual) canals were selected. A 4-mm apical segment of K3 file size 25/.06 was fractured in each mesiobuccal and mesiolingual canal 5 mm apically from the canal orifice...
November 12, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27780580/antibacterial-efficacy-of-a-new-sonic-irrigation-device-for-root-canal-disinfection
#7
Klaus W Neuhaus, Melanie Liebi, Simone Stauffacher, Sigrun Eick, Adrian Lussi
INTRODUCTION: Passive ultrasonic irrigation (PUI) is the most widespread method used to activate irrigation solutions. Concerns have been raised that PUI is less effective in curved root canals and is not passive at all. Our aim was to compare a novel passive sonic irrigation (PSI) device (6000 Hz) with PUI and manual irrigation (MI) with respect to their efficiency in removing different endodontic microorganisms from curved and straight root canals. METHODS: We performed 2 experiments as follows...
October 22, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27776886/bending-resistance-and-cyclic-fatigue-life-of-reciproc-unicone-and-waveone-reciprocating-instruments
#8
Emmanuel João Nogueira Leal Silva, Laise Silva Villarino, Victor Talarico Leal Vieira, Thais Accorsi-Mendonça, Henrique Dos Santos Antunes, Gustavo De-Deus, Hélio Pereira Lopes
INTRODUCTION: This study evaluated the bending resistance and cyclic fatigue life of a new single-file reciprocating instrument (Unicone; Medin, Nové Město na Moravě, Czech Republic). Reciproc (VDW, Munich, Germany) and WaveOne (Dentsply Maillefer) instruments were used as references for comparison. METHODS: Flexibility was determined by 45° bending tests using a universal testing machine. The cyclic fatigue test was performed using a custom-made device. For this test, an artificial canal with a 60° angle and a 5-mm radius of curvature was used...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27776885/understanding-external-cervical-resorption-in-vital-teeth
#9
Athina M Mavridou, Esther Hauben, Martine Wevers, Evert Schepers, Lars Bergmans, Paul Lambrechts
INTRODUCTION: The aim of this study was to investigate the 3-dimensional (3D) structure and the cellular and tissue characteristics of external cervical resorption (ECR) in vital teeth and to understand the phenomenon of ECR by combining histomorphological and radiographic findings. METHODS: Twenty-seven cases of vital permanent teeth displaying ECR were investigated. ECR diagnosis was based on clinical and radiographic examination with cone-beam computed tomographic imaging...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27776884/antimicrobial-activity-and-physicochemical-properties-of-calcium-hydroxide-pastes-used-as-intracanal-medication
#10
Rafaela Fernandes Zancan, Rodrigo Ricci Vivan, Marcelo Ribeiro Milanda Lopes, Paulo Henrique Weckwerth, Flaviana Bombarda de Andrade, José Burgos Ponce, Marco Antonio Hungaro Duarte
INTRODUCTION: The aim of the present study was to evaluate the pH, calcium release, solubility, and antimicrobial action against biofilms of calcium hydroxide + saline solution, Calen (SS White Artigos Dentários Ltd, Rio de Janeiro, Brazil) (CH/P), Calen camphorated paramonochlorophenol (CMCP) (CH/CMPC), and calcium hydroxide + chlorhexidine (CH/CHX) pastes. METHODS: The pH of the pastes was determined with a calibrated pH meter placed in direct contact with each paste...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27776883/gene-expression-profiling-and-molecular-signaling-of-various-cells-in-response-to-tricalcium-silicate-cements-a-systematic-review
#11
Elanagai Rathinam, Sivaprakash Rajasekharan, Ravi Teja Chitturi, Heidi Declercq, Luc Martens, Peter De Coster
INTRODUCTION: The aim of this study was to present a systematic review investigating the gene expression of various cells (other than dental pulp cells) in response to different variants of tricalcium silicate cements (TSCs). METHODS: A systematic search of the literature was performed by 2 independent reviewers followed by article selection and data extraction. Studies analyzing any cell type except dental pulp stem cells and any variant of tricalcium silicate cement either as the experimental or as the control group were included...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27776882/zinc-oxide-nanoparticles-enhance-physicochemical-characteristics-of-grossman-sealer
#12
Marco Aurélio Versiani, Fuad Jacob Abi Rached-Junior, Anil Kishen, Jesus Djalma Pécora, Yara Terezinha Silva-Sousa, Manoel Damião de Sousa-Neto
INTRODUCTION: Metallic antibacterial nanoparticles have been shown to provide distinct antibacterial advantage and antibiofilm efficacy when applied in infected root canals. The purpose of this study was to evaluate the influence of incorporating zinc oxide nanoparticles (ZnO-Np) on the physicochemical properties of Grossman sealer. METHODS: Grossman sealer was prepared according to its original formula. Additionally, 4 experimental sealers were prepared by replacing the zinc oxide component of the powder with ZnO-Np (average size of 20 nm) in different amounts (25%, 50%, 75%, and 100%)...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27776881/anxiety-related-to-nonsurgical-root-canal-treatment-systematic-review
#13
Sameera Khan, Reza Hamedy, Yuejuan Lei, Rikke S Ogawa, Shane N White
INTRODUCTION: Anxiety is an emotion characterized by feelings of tension, worried thoughts, and physical changes. Dental anxiety has been associated with pain, fear, care avoidance, and more invasive treatments including nonsurgical root canal treatment (NSRCT). The very words root canal are deeply embedded in societal consciousness. Better understanding of dental anxiety may prevent treatment avoidance. The purpose of this study was to conduct a systematic review of NSRCT-associated anxiety...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27776880/guided-autotransplantation-of-teeth-a-novel-method-using-virtually-planned-3-dimensional-templates
#14
Georg D Strbac, Albrecht Schnappauf, Katharina Giannis, Michael H Bertl, Andreas Moritz, Christian Ulm
INTRODUCTION: The aim of this study was to introduce an innovative method for autotransplantation of teeth using 3-dimensional (3D) surgical templates for guided osteotomy preparation and donor tooth placement. METHODS: This report describes autotransplantation of immature premolars as treatment of an 11-year-old boy having suffered severe trauma with avulsion of permanent maxillary incisors. This approach uses modified methods from guided implant surgery by superimposition of Digital Imaging and Communications in Medicine files and 3D data sets of the jaws in order to predesign 3D printed templates with the aid of a fully digital workflow...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27776879/expression-profiles-of-inflammation-associated-micrornas-in-periapical-lesions-and-human-periodontal-ligament-fibroblasts-inflammation
#15
Junli Yue, Dongzhe Song, Wanlu Lu, Ying Lu, Wei Zhou, Xuelian Tan, Lan Zhang, Dingming Huang
INTRODUCTION: microRNAs (miRNAs) have been identified to be closely related to inflammatory diseases. The aim of our study was to identify expression profiles of miRNAs associated with inflammation in apical periodontitis (AP) lesions and human periodontal ligament fibroblasts (HPDLFs) inflammation. METHODS: Total RNAs were extracted from 10 AP lesions, 6 control tissues, and HPDLFs using lysis buffer. Expressions of miRNAs (miR-29b, 106b, 125b, 143, 155, and 198) were detected by real-time polymerase chain reaction...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27776878/evaluation-of-the-cyclic-fatigue-and-torsional-resistance-of-novel-nickel-titanium-rotary-files-with-various-alloy-properties
#16
Mehmet Emin Kaval, Ismail Davut Capar, Hüseyin Ertas
INTRODUCTION: The purpose of this study was to evaluate the cyclic fatigue and torsional resistance of Hyflex EDM, ProTaper Gold (PTG), and ProTaper Universal (PTU) instruments. METHODS: For the cyclic fatigue test, 10 instruments from each group were tested by using a stainless steel block with 1.5-mm diameter and 3-mm radius of 60° angle of curvature. The instruments were allowed to rotate by using a low-torque motor, and number of cycles from the beginning to the fracture was recorded...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27776877/influence-of-warm-vertical-compaction-technique-on-physical-properties-of-root-canal-sealers
#17
Wei Qu, Wei Bai, Yu-Hong Liang, Xue-Jun Gao
INTRODUCTION: The aim of this study was to evaluate the influence of warm vertical compaction on the physical properties of root canal sealers. METHODS: The physical properties of 4 sealers (zinc oxide -eugenol [ZOE], AH Plus [Dentsply International, York, PA], RoekoSeal [Roeko/Coltene/Whaledent, Langenau, Germany], and iRoot SP [Innovative Bioceramix, Vancouver, Canada]) were tested. The setting time and flow of these sealers were measured at standard and high temperatures using ISO 6876 (2012) specifications...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27776876/comparison-of-the-potential-discoloration-effect-of-bioaggregate-biodentine-and-white-mineral-trioxide-aggregate-on-bovine-teeth-in%C3%A2-vitro-research
#18
Selen Esin Yoldaş, Mehmet Bani, Didem Atabek, Haluk Bodur
INTRODUCTION: Tricalcium silicate cements can be used for pulp capping, pulpotomies, apical barrier formation in teeth with open apices, repair of root perforations, regenerative endodontics, and root canal filling. The aim of this study was to evaluate and compare the discoloration potential of 3 different tricalcium cements using a bovine tooth model. METHODS: Forty bovine anterior teeth have been used for the study. Crowns separated from the roots were randomly divided into 4 groups: the BioAggregate (IBC, Vancouver, Canada) group, the Biodentine (Septodont, Saint Maur des Fosses, France) group, the mineral trioxide aggregate Angelus (Angelus, Londrina, PR, Brazil) group, and the only blood group...
October 21, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27769680/evaluation-of-root-canal-dentin-erosion-after-different-irrigation-methods-using-energy-dispersive-x-ray-spectroscopy
#19
Zhejun Wang, Hazuki Maezono, Ya Shen, Markus Haapasalo
INTRODUCTION: The purpose of this study was to examine the level of erosion in root dentin caused by different irrigation methods and protocols. METHODS: Thirty-five extracted upper molar teeth were instrumented and divided into 7 groups to undergo treatment by different methods: negative control, GentleWave System (Sonendo Inc, Laguna Hills, CA), and syringe needle irrigation following different protocols. The teeth were instrumented to size #25/.08 or #30/.09 for needle irrigation groups and to ProTaper size S1 for the GentleWave group under 5% sodium hypochlorite (NaOCl)...
October 18, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27769679/maxillary-sinus-impaction-of-a-core-carrier-causing-sustained-apical-periodontitis-sinusitis-and-nasal-stenosis-a-3-year-follow-up
#20
Lars Bjørndal, Catharina Amaloo, Merete Markvart, Vibe Rud, Klaus Qvortrup, Camilla Stavnsbjerg, Thomas Bjarnsholt
INTRODUCTION: The aim was to present a case report of a full-length extrusion of an obturator's core carrier into the maxillary sinus, causing clinical symptoms from the nose region with differential diagnostics aspects, which, in turn, led to several surgical treatments of the nostrils before diagnosis and correct endodontic retreatment of a maxillary right first molar. A 36-year-old man presented in 2012 with complaints from the right nostril region. Medical treatment with antibiotics and surgical procedures because of nasal stenosis resulted only in partial improvement...
October 18, 2016: Journal of Endodontics
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