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Revista do Instituto de Medicina Tropical de São Paulo

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https://www.readbyqxmd.com/read/27982358/alternative-methods-for-sequencing-full-tspyv-genomes-using-sanger-or-ngs
#1
LETTER
Paulo Roberto Urbano, Ana Carolina Soares de Oliveira, Camila Malta Romano
No abstract text is available yet for this article.
December 8, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27982357/reporting-delay-during-the-yellow-fever-outbreak-angola-2016
#2
LETTER
Erika Valeska Rossetto, Expedito José de Albuquerque Luna
No abstract text is available yet for this article.
December 8, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27982356/vertical-transmission-of-dengue-infection-the-first-putative-case-reported-in-china
#3
Xueru Yin, Xiaozhu Zhong, Shilei Pan
Dengue is a systemic viral infection that is commonly transmitted between humans via mosquitoes. Other modes of transmission such as the vertical one are rare and have been infrequently reported in the literature. This report investigates one case of vertical transmission of dengue in Guangzhou, China. A G1P1 lady at 39 weeks of gestation was referred to the Huzhong Hospital presenting a fever for two days. She subsequently developed a skin rash on the back and lower limb and at that time she had already experienced five days of fever...
December 8, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27982355/detection-of-human-anti-zika-virus-igg-by-elisa-using-an-antigen-from-in-vitro-infected-vero-cells-preliminary-results
#4
Laura Masami Sumita, Jaqueline Polizeli Rodrigues, Noely Evangelista Ferreira, Alvina Clara Felix, Nathalia Caroline Santiago Souza, Clarisse Martins Machado, Heitor Franco de Andrade Júnior
Zika virus (ZKV) infection is a huge public health problem in Brazil because of the increased incidence of microcephaly in neonates from infected mothers. Detection of specific IgG antibodies in maternal serum samples constitutes an important approach for diagnosing ZKV infection and evaluating its relationship with neonatal microcephaly. However, as there is no serological test produced in Brazil to detect IgM and IgG antibodies against ZKV, we sought to examine specific IgG in serum samples from patients or suspected mothers to detect previous infection and to test for specificity with regard to flaviviral infections occurring in the same area...
December 8, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27982354/genetic-mutations-affecting-the-first-line-eradication-therapy-of-helicobacter-pylori-infected-egyptian-patients
#5
Iman Ramzy, Hassan Elgarem, Iman Hamza, Doaa Ghaith, Tamer Elbaz, Waleed Elhosary, Gehan Mostafa, Mohammad A Mohey Eldin Elzahry
Introduction: Several genetic mutations affect the first-line triple therapy for Helicobacter pylori. We aimed to study the most common genetic mutations affecting the metronidazole and clarithromycin therapy for H. pylori-infected Egyptian patients. Patients and Methods: In our study, we included 100 successive dyspeptic patients scheduled for diagnosis through upper gastroscopy at Cairo's University Hospital, Egypt. Gastric biopsies were tested for the presence of H...
December 8, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27982353/sand-flies-diptera-psychodidae-in-an-endemic-area-of-leishmaniasis-in-aquidauana-municipality-pantanal-of-mato-grosso-do-sul-brazil
#6
Helen Rezende de Figueiredo, Mirella Ferreira da Cunha Santos, Aline Etelvina Casaril, Jucelei Oliveira de Moura Infran, Leticia Moraes Ribeiro, Carlos Eurico Dos Santos Fernandes, Alessandra Gutierrez de Oliveira
The Aquidauana municipality is considered an endemic area of leishmaniasis and an important tourist site in Mato Grosso do Sul State. The aim of this study was to investigate the sand fly fauna in the city of Aquidauana. Captures were carried out twice a month, from April 2012 to March 2014 with automatic light traps and active aspiration, in the peridomicile and domicile of six residences. A total of 9,338 specimens were collected, 3,179 and 6,159 using light traps and active aspiration, respectively. The fauna consisted of: Brumptomyia brumpti, Evandromyia aldafalcaoae, Ev...
December 8, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27982352/risk-factors-associated-with-american-cutaneous-leishmaniasis-in-an-endemic-area-of-brazil
#7
Alberon Ribeiro de Araujo, Nairomberg Cavalcanti Portela, Ana Paula Sampaio Feitosa, Otamires Alves da Silva, Ricardo Andrade Arraes Ximenes, Luiz Carlos Alves, Fábio André Brayner
Brazil is among the top five countries worldwide regarding the number of cases of leishmaniasis, which are present in all of the regions of the country. The northeastern region continues to have higher numbers of cases every year and in the state of Pernambuco, 34% of the municipalities are endemic for this disease. The diversity of vectors, reservoirs and etiological agents, in association with socioeconomic and environmental conditions, gives rise to factors that can modify the behavior of American cutaneous leishmaniasis...
December 8, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828626/ocular-complications-caused-by-cryptococcus-gattii-aflp4-vgi-meningitis-in-an-immunocompetent-host
#8
LETTER
Chang-Hua Chen, Shao-Hung Wang, Wei Liang Chen, Wang-Fu Wang, San-Ni Chen
No abstract text is available yet for this article.
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828625/clinical-management-of-localized-bcg-adverse-events-in-children
#9
Thais das Neves Fraga Moreira, Maria Isabel de Moraes-Pinto, Beatriz Tavares Costa-Carvalho, Anete Sevciovic Grumach, Lily Yin Weckx
BCG adverse events (BCG-AE) are rare conditions with no well-established treatment. This study aims to describe clinical characteristics and outcome of localized BCG-AE. Children with BCG-AEs who were treated at the Reference Center for Special Immunobiologicals of the Federal University of São Paulo from 2009 to 2011 were included. Patients were followed monthly until 3 months after healing. One hundred and twenty-seven patients with localized BCG-AE were followed: 67 (52.7%) had suppurative lymphadenitis; 30 (23...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828624/antimicrobial-susceptibility-of-streptococcus-agalactiae-isolated-from-pregnant-women
#10
Simone Cristina Castanho Sabaini de Melo, Nathally Claudiane de Souza Santos, Marcia de Oliveira, Regiane Bertin de Lima Scodro, Rosilene Fressatti Cardoso, Rúbia Andreia Falleiros Pádua, Flavia Teixeira Ribeiro Silva, Aline Balandis Costa, Maria Dalva de Barros Carvalho, Sandra Marisa Pelloso
Introduction: Group B streptococcus (GBS) or Streptococcus agalactiae can colonize the gastrointestinal and genitourinary tracts and has been considered one of the most important risk factors for the development of neonatal disease. The present study evaluated the antimicrobial susceptibility of GBS isolates from pregnant women who were attended at a public health service in Northern Paraná, Brazil. Methods: A descriptive analytical cross-sectional study was performed with 544 pregnant women, at ≥ 35 weeks of gestation...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828623/adherence-to-influenza-vaccination-among-medical-students-during-and-after-influenza-a-h1n1-pandemic
#11
Stéfano Ivani de Paula, Gustavo Ivani de Paula, Kelly Simone Almeida Cunegundes, Maria Isabel de Moraes-Pinto
This study evaluated the adherence to influenza vaccination among medical students in 2010 and 2011. From August to December 2011, a questionnaire was used to record the influenza vaccination in 2010 and 2011, reasons for acceptance of the influenza vaccine and knowledge of healthcare workers about the influenza vaccine recommendation. One hundred and forty-four students from the 2ndto the 6th years of the medical school were interviewed. A great adherence to pandemic influenza vaccine was noted in 2010, (91% of the students), with "self-protection" being the most common reason cited for vaccination...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828622/sapoviruses-in-children-with-acute-gastroenteritis-from-manaus-amazon-region-brazil-2010-2011
#12
Tammy Kathlyn Amaral Reymão, Juliana das Merces Hernandez, Samya Thalita Picanço da Costa, Maísa Silva de Sousa, Darleise de Souza Oliveira, Luciana Damascena da Silva, Renato da Silva Bandeira, Ian Carlos Gomes de Lima, Luana da Silva Soares, Joana Darc Pereira Mascarenhas, Yvone Benchimol Gabbay
Sapoviruses (SaVs) are responsible for acute gastroenteritis in humans, especially children and the elderly. In Brazil, data on SaVs infections are very limited, especially in Northern Brazil. Here, we investigated the occurrence of SaVs in samples from hospitalized children under ten years old that presented acute gastroenteritis. Positive samples were genotyped and phylogenetic analysis was performed using prototype strains sequences obtained from GenBank database. In total, 156 fecal samples were screened by RT-PCR for SaVs...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828621/seroprevalence-of-htlv-in-a-population-of-hiv1-infected-patients-in-midwestern-brazil
#13
Aline Garcia Kozlowski, Márcia Alves Dias de Matos, Megmar Aparecida Dos Santos Carneiro, Carmen Luci Rodrigues Lopes, Sheila Araújo Teles, Carolina Paulo Vicente, Regina Maria Bringel Martins
Human T-cell lymphotropic virus (HTLV) may affect the clinical course of human immunodeficiency virus 1 (HIV1). Both infections are common in endemic areas because these viruses share similar routes of transmission. The aim of this study was to estimate the seroprevalence of HTLV1/2 in a population of HIV1-infected patients in the state of Goiás, Midwestern Brazil. Of the 505 studied patients, four (0.79%) were positive for anti-HTLV1/2 by enzyme-linked immunosorbent assay (ELISA), with HTLV1 infection confirmed by line immunoassay (LIA) and polymerase chain reaction (PCR) in all of the ELISA-positive samples...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828620/prevalence-of-american-trypanosomiasis-and-leishmaniases-in-domestic-dogs-in-a-rural-area-of-the-municipality-of-s%C3%A3-o-jo%C3%A3-o-do-piau%C3%A3-piau%C3%A3-state-brazil
#14
Taliha Dias Perez, Fabiano Borges Figueiredo, Artur Augusto Mendes Velho Junior, Valmir Laurentino Silva, Maria de Fátima Madeira, Reginaldo Peçanha Brazil, José Rodrigues Coura
Chagas disease and the leishmaniases are endemic zoonoses of great importance to public health in the state of Piauí, Brazil. The domestic dog (Canis familiaris) is a major reservoir, host of Trypanosoma cruzi and Leishmania spp. in both urban and rural areas, playing an important role in the transmission of these parasites. The present study evaluated the prevalence of both infectious diseases in dogs of a rural area in the municipality of São João do Piauí, Piauí State. One hundred twenty-nine blood samples were collected for serological assessment: for the leishmaniases, 49 (38%) animals tested positive by the Dual-Path Platform technology (DPP), nine (6%) by the Enzyme-linked Immunosorbent Assay (ELISA), and 19 (14...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828619/a-descriptive-study-of-pandemic-influenza-a-h1n1-pdm09-in-brazil-2009-2010
#15
Erika Valeska Rossetto, Expedito José de Albuquerque Luna
Influenza A viruses undergo frequent antigenic mutations and may thus cause seasonal epidemics and pandemics. The aim of this study was to recover the epidemiological history of the pandemic influenza A(H1N1)pdm09 in Brazil. A descriptive study was conducted in 2009-2010. The Brazilian Information System for reportable diseases (SINAN) was the data source. A total of 105,054 suspected cases of influenza A(H1N1)pdm09 were reported to SINAN. Of these, 53,797 (51.2%) were classified as the new influenza virus subtype...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828618/a-decade-trend-of-multidrug-resistant-tuberculosis-in-s%C3%A3-o-paulo-state-brazil
#16
Valdes Roberto Bollela, Fernanda Guioti Puga, Maria Janete Moya, Mauro Andrea, Maria de Lourdes Viude Oliveira
The aim of this retrospective study was to review all the notified cases of multidrug-resistant tuberculosis (MDR-TB) in São Paulo State (Brazil), as well as to describe and discuss the clinical, microbiological and radiologic aspects in a single reference center, within the same state, from 2000 to 2012. There were 1,097 notifications of MDR-TB in São Paulo State over this period, 70% affecting men aged on average 38 years (10-77). There was a significant fall in the MDR-TB mortality rate from 30% to 8% (2000-2003 versus 2009-2012)...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828617/dentists-knowledge-and-experience-regarding-leprosy-in-an-endemic-area-in-brazil
#17
Ronald Jefferson Martins, Maria Emília Oliveira Gomes Carloni, Suzely Adas Saliba Moimaz, Cléa Adas Saliba Garbin, Artênio José Ísper Garbin
This study aims to analyze the dental surgeons' knowledge about leprosy and its ways of transmission, clinical characteristics and treatment, besides analyzing their experience with respect to diagnostic suspicion and case referrals. The study population comprised 242 dental surgeons working in the public dental service of the city of Cuiabá, Mato Grosso, Brazil. A self-applicable questionnaire containing questions about the dental surgeon's profile was used, including his/her knowledge on leprosy, as well as his/her practices concerning the disease...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828616/leishmania-infantum-infection-in-dogs-from-the-southern-region-of-minas-gerais-state-brazil
#18
Juliana Barbosa Nunes, Márcia Dalastra Laurenti, Herminia Yohko Kanamura, Alessandro Antônio Costa Pereira, Fabio Antonio Colombo, Marcos José Marques
Visceral leishmaniasis is a systemic and chronic disease and dogs are the main reservoir of the etiologic agent, Leishmania infantum (syn L. chagasi). A serological and molecular investigation of canine visceral leishmaniasis (CVL) was performed in the municipality of Alfenas, located in the southern region of Minas Gerais, where the disease is not endemic. Samples from 87 dogs were submitted to serological tests including the Dual Path Platform (DPP (r) ) CVL Bio-Manguinhos rapid test, an in-house enzyme-linked immunosorbent assay (ELISA) and an immunofluorescence antibody test (IFAT), as well as molecular techniques such as a conventional polymerase chain reaction (PCR) with the RV1/RV2 primers and a quantitative PCR (qPCR) with the LinJ31, Ldon and DNApol primers...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27828615/first-molecular-detection-and-vp7-g-genotyping-of-group-a-rotavirus-by-semi-nested-rt-pcr-from-sewage-in-nigeria
#19
Babatunde Olanrewaju Motayo, Adekunle Johnson Adeniji, Adedayo Omotayo Faneye
Rotavirus is the leading cause of viral gastroenteritis worldwide, and sewage is a major source of the virus dissemination in the environment. Our aim was to detect and genotype rotaviruses from sewages in Nigeria. One hundred and ninety sewage samples were collected between June 2014 and January 2015. The two phase concentration method using PEG 6000 and dextran was used to concentrate sewage samples following WHO protocols. Molecular detection was performed by RT-PCR, and VP7 genotyping by semi-nested multiplex PCR...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27680173/pancreatic-toxicity-as-an-adverse-effect-induced-by-meglumine-antimoniate-therapy-in-a-clinical-trial-for-cutaneous-leishmaniasis
#20
Marcelo Rosandiski Lyra, Sonia Regina Lambert Passos, Maria Inês Fernandes Pimentel, Sandro Javier Bedoya-Pacheco, Cláudia Maria Valete-Rosalino, Erica Camargo Ferreira Vasconcellos, Liliane Fatima Antonio, Mauricio Naoto Saheki, Mariza Mattos Salgueiro, Ginelza Peres Lima Santos, Madelon Noato Ribeiro, Fatima Conceição-Silva, Maria Fatima Madeira, Jorge Luiz Nunes Silva, Aline Fagundes, Armando Oliveria Schubach
American tegumentary leishmaniasis is an infectious disease caused by a protozoan of the genus Leishmania. Pentavalent antimonials are the first choice drugs for cutaneous leishmaniasis (CL), although doses are controversial. In a clinical trial for CL we investigated the occurrence of pancreatic toxicity with different schedules of treatment with meglumine antimoniate (MA). Seventy-two patients were allocated in two different therapeutic groups: 20 or 5 mg of pentavalent antimony (Sb5+)/kg/day for 20 or 30 days, respectively...
September 22, 2016: Revista do Instituto de Medicina Tropical de São Paulo
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