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Archives of Virology

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https://www.readbyqxmd.com/read/28434100/complete-genome-sequence-of-jacquemontia-yellow-vein-virus-a-novel-begomovirus-infecting-jacquemontia-tamnifolia-in-venezuela
#1
Elvira Fiallo-Olivé, Dorys T Chirinos, Francis Geraud-Pouey, Jesús Navas-Castillo
Wild plants of the family Convolvulaceae are hosts for a few New World begomoviruses (genus Begomovirus, family Geminiviridae). In this work, we report the complete genome sequence of a new begomovirus infecting the wild convolvulaceous plant Jacquemontia tamnifolia in Venezuela. The cloned bipartite genome showed the organization of typical New World begomoviruses and was found to be phylogenetically related to those of begomoviruses from Venezuela and other Caribbean countries. Several recombination events have been shown to have occurred involving genome fragment exchange with related begomoviruses infecting crops such as tomato and cucurbits and wild plants, including Jacquemontia sp...
April 22, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28434099/the-complete-genome-sequence-of-a-novel-virus-bellflower-veinal-mottle-virus-suggests-the-existence-of-a-new-genus-within-the-family-potyviridae
#2
Jang-Kyun Seo, Hae-Ryun Kwak, Mi-Kyeong Kim, Jeong-Soo Kim, Hong-Soo Choi
A new virus was isolated from a bellflower (Campanula takesimana) plant showing veinal mottle symptoms, and its complete genome sequence was determined. The viral genome consists of a positive-sense single-stranded RNA of 8,259 ribonucleotides. Electron microscopic observation revealed that the viral genome is packaged as a filamentous particle with an average length of approximately 760 nm. BLAST searches of protein databases showed that the encoded polyprotein has a maximum amino acid sequence identity of 34...
April 22, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28434098/changes-to-taxonomy-and-the-international-code-of-virus-classification-and-nomenclature-ratified-by-the-international-committee-on-taxonomy-of-viruses-2017
#3
Michael J Adams, Elliot J Lefkowitz, Andrew M Q King, Balázs Harrach, Robert L Harrison, Nick J Knowles, Andrew M Kropinski, Mart Krupovic, Jens H Kuhn, Arcady R Mushegian, Max Nibert, Sead Sabanadzovic, Hélène Sanfaçon, Stuart G Siddell, Peter Simmonds, Arvind Varsani, Francisco Murilo Zerbini, Alexander E Gorbalenya, Andrew J Davison
This article lists the changes to virus taxonomy approved and ratified by the International Committee on Taxonomy of Viruses (ICTV) in March 2017.
April 22, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28432563/complete-sequences-of-the-rna-1-and-rna-2-segments-of-chronic-bee-paralysis-virus-strain-cbpv-bj-detected-in-china
#4
Beibei Li, Shuai Deng, Dahe Yang, Chunsheng Hou, Qingyun Diao
Chronic bee paralysis virus (CBPV) is an important viral pathogen that affects adult bees. Although several CBPV strains have been reported, little information has been obtained from China. In this study, two major segments of the CBPV Chinese isolate CBPV-BJ, RNA 1 and RNA 2, were determined to be 3657 and 2267 nucleotides (nt) in length, respectively. RNA 1 and RNA 2 contained three and four open reading frames (ORFs), respectively, which agreed with known reference strains (EU122229 and EU122230). The RNA 1 had 98% nucleotide sequence identity to a known Chinese strain (KU950353), and RNA 2 had 97% nucleotide sequence identity to another Chinese strain (KU950354)...
April 21, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28429133/predominance-of-new-g9p-8-rotaviruses-closely-related-to-turkish-strains-in-nizhny-novgorod-russia
#5
T A Sashina, O V Morozova, N V Epifanova, N A Novikova
Genotype G9P[8] rotaviruses are rare in the territory of Russia. They were found in Nizhny Novgorod only in 2011-2012 for the first time, when their proportion was 25.9%. During the next two seasons, G9P[8] strains were detected in only 1.8% of cases. Their proportion substantially increased again in 2014, and they became predominant in the city by 2016. Phylogenetic analysis on the basis of gene VP7 nucleotide sequences showed that this increase was accompanied by the emergence of new strains in the population...
April 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28429132/development-of-a-real-time-recombinase-polymerase-amplification-assay-for-rapid-and-sensitive-detection-of-porcine-circovirus-2
#6
Jianchang Wang, Jinfeng Wang, Libing Liu, Wanzhe Yuan
Porcine diseases associated with porcine circovirus 2 (PCV-2) infection have resulted in significant economic losses worldwide. A real-time recombinase polymerase amplification (RPA) assay was developed to detect PCV-2 using primers and an exo probe specific for the ORF2 gene. The reaction process can be completed in 20 min at 38 °C. The assay only detects PCV-2, as there was no cross-reaction with other pathogens important in pigs. Using the PCV-2 genomic DNA as template, the analytical sensitivity of the real-time RPA was 103 copies...
April 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28429131/the-first-comprehensive-molecular-detection-of-six-honey-bee-viruses-in-iran-in-2015-2016
#7
Mohammadreza Ghorani, Omid Madadgar, Arash Ghalyanchi Langeroudi, Mohammadreza Rezapanah, Sedigheh Nabian, Hesameddin Akbarein, Reza Kh Farahani, Hossein Maghsoudloo, Hamed Abdollahi, Mohammad Forsi
At least 18 viruses have been reported in the honey bee (Apis mellifera L.). However, severe diseases in honey bees are mainly caused by six viruses, and these are the most important in beekeeping. These viruses include: deformed wing virus (DWV), acute bee paralysis virus (ABPV), chronic bee paralysis virus (CBPV), sacbrood virus (SBV), kashmir bee virus (KBV), and black queen cell virus (BQCV). In this study, we evaluated 89 Iranian honey bee apiaries (during the period 2015-2016) suffering from symptoms of depopulation, sudden collapse, paralysis, or dark coloring, by employing reverse transcription-PCR...
April 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28424887/a-metagenomics-study-for-the-identification-of-respiratory-viruses-in-mixed-clinical-specimens-an-application-of-the-iterative-mapping-approach
#8
Yu-Nong Gong, Shu-Li Yang, Guang-Wu Chen, Yu-Wen Chen, Yhu-Chering Huang, Hsiao-Chen Ning, Kuo-Chien Tsao
Metagenomic approaches to detect viral genomes and variants in clinical samples have various challenges, including low viral titers and bacterial and human genome contamination. To address these limitations, we examined a next-generation sequencing (NGS) and iterative mapping approach for virus detection in clinical samples. We analyzed 40 clinical specimens from hospitalized children diagnosed with acute bronchiolitis, croup, or respiratory tract infections in which virus identification by viral culture or polymerase chain reaction (PCR) was unsuccessful...
April 19, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28424886/detection-of-koi-herpesvirus-khv-using-a-monoclonal-antibody-against-cyprinus-carpio-igm
#9
Yingying Li, Shucheng Zheng, Qing Wang, Sven M Bergmann, Weiwei Zeng, Yingying Wang, Chun Liu, Cunbin Shi
Koi herpesvirus disease (KHVD) is associated with high mortality in both common carp and koi carp (Cyprinus carpio L.) worldwide. The indirect detection of fish viruses based on the identification of antibodies has emerged as a practical and reliable means of diagnosis. Thus, it is important to create monoclonal antibodies (MAbs) against carp IgM. By using hybridoma-monoclonal antibody technology, one hybridoma cell line secreting MAbs against IgM from carp was established. In western blot analysis, the secreted MAb from cell line A5-E10 recognized the heavy chain of IgM from common carp or koi but did not react with immunoglobulins from three different fish species: grass carp (Ctenopharyngodon idella), tilapia (Oreochromis mossambicus) and Mandarin fish (Siniperca chuatsi)...
April 19, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28421368/recombinant-foot-and-mouth-disease-virus-fmdv-non-structural-protein-3a-fused-to-enhanced-green-fluorescent-protein-egfp-as-a-candidate-probe-to-identify-fmdv-infected-cattle-in-serosurveys
#10
Cecilia M Lotufo, Ingrid E Bergmann, Nora M Mattion, Maximiliano Wilda, Pablo R Grigera
Recombinant protein 3A-EGFP, a fusion construct between foot-and-mouth disease virus (FMDV) non-structural protein 3A and the enhanced green fluorescent protein (EGFP) was expressed in BL21-DE3 cells. The identity of the partially purified protein 3A-EGFP was confirmed by its reactivity with sera from cattle infected with FMDV and with a monoclonal antibody specific for FMDV-3ABC (MAb3H7) in Western blot assays. No reactivity was observed with sera from uninfected vaccinated animals. The performance of 3A-EGFP as an antigen in an indirect enzyme-linked immunosorbent assay (ELISA) was assessed and compared with that of a previously developed and validated capture ELISA that uses a 3ABC recombinant antigen (3ABC ELISA) and has been widely applied for serological surveys in Argentina...
April 18, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28421367/ns3-protease-resistance-associated-substitutions-in-liver-tissue-and-plasma-samples-from-patients-infected-by-hepatitis-c-virus-genotype-1a-or-1b
#11
Giulia Morsica, Andrea Andolina, Marco Merli, Emanuela Messina, Hamid Hasson, Adriano Lazzarin, Caterina Uberti-Foppa, Sabrina Bagaglio
The presence of naturally occurring resistance-associated substitutions (RASs) in the HCV-protease domain has been poorly investigated in the liver, the main site of HCV replication. We evaluated the natural resistance of the virus to NS3 protease inhibitors in liver tissue and plasma samples taken from HCV-infected patients. RASs were investigated by means of viral population sequencing in liver tissue samples from 18 HCV-infected patients harbouring genotype 1a or genotype 1b; plasma samples from 12 of these patients were also available for virological investigation...
April 18, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28421366/two-deletion-variants-of-middle-east-respiratory-syndrome-coronavirus-found-in-a-patient-with-characteristic-symptoms
#12
Qian Xie, Yujuan Cao, Juan Su, Jie Wu, Xianbo Wu, Chengsong Wan, Mingliang He, Changwen Ke, Bao Zhang, Wei Zhao
Significant sequence variation of Middle East respiratory syndrome coronavirus (MERS CoV) has never been detected since it was first reported in 2012. A MERS patient came from Korea to China in late May 2015. The patient was 44 years old and had symptoms including high fever, dry cough with a little phlegm, and shortness of breath, which are roughly consistent with those associated with MERS, and had had close contact with individuals with confirmed cases of MERS.After one month of therapy with antiviral, anti-infection, and immune-enhancing agents, the patient recovered in the hospital and was discharged...
April 18, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28421365/grass-carp-reovirus-outer-capsid-proteins-vp5-and-vp7-interact-in-vitro
#13
Weisha Liu, Hao Wang, Fei Yu, Liqun Lu
Grass carp reovirus (GCRV) causes viral hemorrhagic disease in cultured grass carp. However, there is no effective means of controlling GCRV. Previous studies have shown, by cryoelectron microscopy, that the outer capsid of GCRV is composed of 200 trimers of VP5-VP7 heterodimers. However, confirmation of this interaction between VP5 and VP7 through molecular biochemistry is still lacking. This study characterized the interactions between VP5 and VP7 in vitro. VP5 was shown to interact with VP7 in a commercial yeast-two-hybrid screen...
April 18, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28409265/genomic-characterization-of-bacteriophage-vb_pcap_pp2-infecting-pectobacterium-carotovorum-subsp-carotovorum-a-new-member-of-a-proposed-genus-in-the-subfamily-autographivirinae
#14
Jeong-A Lim, Sunggi Heu, Jinwoo Park, Eunjung Roh
Bacteriophage vB_PcaP_PP2 (PP2) is a novel virulent phage that infects the plant-pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. PP2 phage has a 41,841-bp double-stranded DNA encoding 47 proteins, and it was identified as a member of the family Podoviridae by transmission electron microscopy. Nineteen of its open reading frames (ORFs) show homology to functional proteins, and 28 ORFs have been characterized as hypothetical proteins. PP2 phage is homologous to Cronobacter phage vB_CskP_GAP227 and Dev-CD-23823...
April 13, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28405766/characterization-of-cross-clade-monoclonal-antibodies-against-h5n1-highly-pathogenic-avian-influenza-virus-and-their-application-to-the-antigenic-analysis-of-diverse-h5-subtype-viruses
#15
Dulyatad Gronsang, Anh N Bui, Dai Q Trinh, Vuong N Bui, Khong V Nguyen, Minh X Can, Tsutomu Omatsu, Tetsuya Mizutani, Makoto Nagai, Yukie Katayama, Rapeewan Thampaisarn, Haruko Ogawa, Kunitoshi Imai
H5N1 highly pathogenic avian influenza viruses (HPAIVs) are a threat to both animal and public health and require specific and rapid detection for prompt disease control. We produced three neutralizing anti-hemagglutinin (HA) monoclonal antibodies (mAbs) using two clades (2.2 and 2.5) of the H5N1 HPAIV isolated in Japan. Blocking immunofluorescence tests showed that each mAb recognized different epitopes; 3B5.1 and 3B5.2 mAbs against the clade 2.5 virus showed cross-clade reactivity to all 26 strains from clades 1, 2...
April 12, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28393307/a-dose-response-study-in-mice-of-a-tetravalent-vaccine-candidate-composed-of-domain-iii-capsid-proteins-from-dengue-viruses
#16
Iris Valdés, Ernesto Marcos, Edith Suzarte, Yusleidi Pérez, Enma Brown, Laura Lazo, Karem Cobas, Melyssa Yaugel, Yadira Rodríguez, Lázaro Gil, Gerardo Guillén, Lisset Hermida
Tetra DIIIC is a subunit vaccine candidate based on domain III of the envelope protein and the capsid protein of the four serotypes of dengue virus. This vaccine preparation contains the DIIIC proteins aggregated with a specific immunostimulatory oligodeoxynucleotide (ODN 39M). Tetra DIIIC has already been shown to be immunogenic and protective in mice and monkeys. In this study, we evaluated the immunogenicity in mice of several formulations of Tetra DIIIC containing different amounts of the recombinant proteins...
April 9, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28389807/taxonomy-of-the-order-mononegavirales-update-2017
#17
Gaya K Amarasinghe, Yīmíng Bào, Christopher F Basler, Sina Bavari, Martin Beer, Nicolás Bejerman, Kim R Blasdell, Alisa Bochnowski, Thomas Briese, Alexander Bukreyev, Charles H Calisher, Kartik Chandran, Peter L Collins, Ralf G Dietzgen, Olga Dolnik, Ralf Dürrwald, John M Dye, Andrew J Easton, Hideki Ebihara, Qi Fang, Pierre Formenty, Ron A M Fouchier, Elodie Ghedin, Robert M Harding, Roger Hewson, Colleen M Higgins, Jian Hong, Masayuki Horie, Anthony P James, Dàohóng Jiāng, Gary P Kobinger, Hideki Kondo, Gael Kurath, Robert A Lamb, Benhur Lee, Eric M Leroy, Ming Li, Andrea Maisner, Elke Mühlberger, Sergey V Netesov, Norbert Nowotny, Jean L Patterson, Susan L Payne, Janusz T Paweska, Michael N Pearson, Rick E Randall, Peter A Revill, Bertus K Rima, Paul Rota, Dennis Rubbenstroth, Martin Schwemmle, Sophie J Smither, Qisheng Song, David M Stone, Ayato Takada, Calogero Terregino, Robert B Tesh, Keizo Tomonaga, Noël Tordo, Jonathan S Towner, Nikos Vasilakis, Viktor E Volchkov, Victoria Wahl-Jensen, Peter J Walker, Beibei Wang, David Wang, Fei Wang, Lin-Fa Wang, John H Werren, Anna E Whitfield, Zhichao Yan, Gongyin Ye, Jens H Kuhn
In 2017, the order Mononegavirales was expanded by the inclusion of a total of 69 novel species. Five new rhabdovirus genera and one new nyamivirus genus were established to harbor 41 of these species, whereas the remaining new species were assigned to already established genera. Furthermore, non-Latinized binomial species names replaced all paramyxovirus and pneumovirus species names, thereby accomplishing application of binomial species names throughout the entire order. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV)...
April 7, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28389806/absence-of-vaccinia-virus-detection-in-a-remote-region-of-the-northern-amazon-forests-2005-2015
#18
Galileu Barbosa Costa, Anne Lavergne, Edith Darcissac, Vincent Lacoste, Betânia Paiva Drumond, Jônatas Santos Abrahão, Erna Geessien Kroon, Benoît de Thoisy, Giliane de Souza Trindade
Vaccinia virus (VACV) circulates in Brazil and other South America countries and is responsible for a zoonotic disease that usually affects dairy cattle and humans, causing economic losses and impacting animal and human health. Furthermore, it has been detected in wild areas in the Brazilian Amazon. To better understand the natural history of VACV, we investigated its circulation in wildlife from French Guiana, a remote region in the Northern Amazon forest. ELISA and plaque reduction neutralization tests were performed to detect anti-orthopoxvirus antibodies...
April 7, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28386650/complete-genomic-characterization-of-milk-vetch-dwarf-virus-isolates-from-cowpea-and-broad-bean-in-anhui-province-china
#19
Chenhua Zhang, Hongying Zheng, Dankan Yan, Kelei Han, Xijiao Song, Yong Liu, Dongfang Zhang, Jianping Chen, Fei Yan
Cowpea and broad bean plants showing severe stunting and leaf rolling symptoms were observed in Hefei city, Anhui province, China, in 2014. Symptomatic plants from both species were shown to be infected with milk vetch dwarf virus (MDV) by PCR. The complete genomes of MDV isolates from cowpea and broad bean were sequenced. Each of them had eight genomic DNAs that differed between the two isolates by 10.7% in their overall nucleotide sequences. In addition, the MDV genomes from cowpea and broad bean were associated with two and three alphasatellite DNAs, respectively...
April 6, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28378295/genetic-characterization-of-orf-virus-associated-with-an-outbreak-of-severe-orf-in-goats-at-a-farm-in-lusaka-zambia-2015
#20
Edgar Simulundu, Nandi Mtine, Thoko F Kapalamula, Masahiro Kajihara, Yongjin Qiu, James Ngoma, Victor Zulu, Geoffrey Kwenda, Chrispin Chisanga, Isaac K Phiri, Ayato Takada, Aaron S Mweene
Orf or contagious ecthyma is a neglected and economically important zoonotic disease caused by a dermatotropic parapoxvirus that commonly affects domestic small ruminants. Although orf is globally distributed, there is a paucity of information on the disease in many African countries. Here, a suspected severe outbreak of orf in goats at a farm in Lusaka was investigated. Orf virus (ORFV) infection was confirmed by PCR amplification of viral DNA (RNA polymerase, B2L and virus interferon-resistance genes) in clinical samples...
April 4, 2017: Archives of Virology
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