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Archives of Virology

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https://www.readbyqxmd.com/read/30631960/intratype-variants-of-the-e2-protein-from-human-papillomavirus-type-18-induce-different-gene-expression-profiles-associated-with-apoptosis-and-cell-proliferation
#1
Alma Mariana Fuentes-González, J Omar Muñoz-Bello, Joaquín Manzo-Merino, Adriana Contreras-Paredes, Abraham Pedroza-Torres, Jorge Fernández-Retana, Carlos Pérez-Plasencia, Marcela Lizano
Persistent infections with high-risk human papillomaviruses (HR-HPVs) are linked to development of cervical cancer due to a deregulation of the productive viral cycle in the host cell, leading to cell transformation. The E2 viral protein is expressed early during an HPV infection and regulates viral replication and transcription. Other functions have been attributed to E2, such as the promotion of apoptosis that are independent of its role in the regulation of the expression of E6 and E7 viral oncogenes. Moreover, it has been shown that the HPV16 E2 protein has regulatory effects on cellular gene expression, suggesting that it participates in the modulation of different cellular processes...
January 10, 2019: Archives of Virology
https://www.readbyqxmd.com/read/30631959/interaction-of-historical-and-modern-sardinian-african-swine-fever-viruses-with-porcine-and-wild-boar-monocytes-and-monocyte-derived-macrophages
#2
Silvia Dei Giudici, Giulia Franzoni, Piero Bonelli, Donatella Bacciu, Giovanna Sanna, Pier Paolo Angioi, Mauro Ledda, Giovannantonio Pilo, Paola Nicolussi, Annalisa Oggiano
African swine fever (ASF) is a contagious viral disease of wild and domestic pigs that is present in many parts of Africa, Asia and Europe, including Sardinia (Italy). Deletions in the EP402R and B602L genes have been found in almost all ASF virus (ASFV) strains circulating in Sardinia from 1990 onwards, and modern Sardinian strains (isolated after 1990) might have acquired some selective advantage compared to historical ones (isolated before 1990). Here, we analysed the host cell responses of wild boars and domestic pigs upon infection with virus variants...
January 10, 2019: Archives of Virology
https://www.readbyqxmd.com/read/30631958/metagenomic-analysis-identifies-human-adenovirus-31-in-children-with-acute-flaccid-paralysis-in-tunisia
#3
Sondes Haddad-Boubaker, Marie-Line Joffret, Philippe Pérot, Mael Bessaud, Zina Meddeb, Hinda Touzi, Françis Delpeyroux, Henda Triki, Marc Eloit
A variety of viruses can cause acute flaccid paralysis (AFP). However, the causative agent, sometimes, remains undetermined. Metagenomics helps in identifying viruses not diagnosed by conventional methods. Stool samples from AFP (n = 104) and non-AFP (n = 114) cases that tested enterovirus-negative by WHO standard methods were investigated. A metagenomics approach, first used on five pools of four samples each, revealed the presence of adenovirus sequences. Amplification in A549 cells and full-genome sequencing were used for complete virus identification and for designing a PCR assay to screen individual related samples...
January 10, 2019: Archives of Virology
https://www.readbyqxmd.com/read/30612200/proteomic-analysis-of-monkey-kidney-llc-mk2-cells-infected-with-a-thai-strain-zika-virus
#4
Thamonwan Diteepeng, Sarawut Khongwichit, Atchara Paemanee, Sittiruk Roytrakul, Duncan R Smith
Zika virus (ZIKV) has been endemic in Southeast Asian countries for several years, but the presence of the virus has not been associated with significant outbreaks of infection unlike other countries around the world where the Asian lineage ZIKV was introduced recently. However, few studies have been undertaken using the endemic virus. The Thai isolate was shown to have a similar tissue tropism to an African isolate of ZIKV, albeit that the Thai isolate infected cells at a lower level as compared to the African isolate...
January 5, 2019: Archives of Virology
https://www.readbyqxmd.com/read/30612199/analysis-of-natural-recombination-and-host-related-evolutionary-dynamics-of-avian-avulavirus-1-isolates-based-on-positive-and-negative-selection-from-1948-to-2017
#5
Majid Esmaelizad, Vafa Mayahi
Adaptation and evolution of avian avulavirus 1, or Newcastle disease virus (NDV), has led to tremendous economic losses worldwide. The occurrence of natural recombination and selection pressure has been traced for NDV based on a few recent reports, but a dominant pattern based on genomic characteristics is lacking. Here, we used bioinformatics tools to search for evidence of recombination in all of the available complete genome sequence of NDV (462 sequences) using RDP4 software. Geographical linkage and host cell relationships of recombinant viruses were also investigated, and a study of the adaptive evolution of avian avulavirus 1 was performed...
January 5, 2019: Archives of Virology
https://www.readbyqxmd.com/read/30607515/editorial
#6
EDITORIAL
Tim Skern
No abstract text is available yet for this article.
January 4, 2019: Archives of Virology
https://www.readbyqxmd.com/read/30604242/development-of-real-time-pcr-based-methods-for-the-detection-of-enzootic-nasal-tumor-virus-2-in-goats
#7
Evangelia D Apostolidi, Dimitra Psalla, Taxiarchis Chassalevris, Serafeim C Chaintoutis, Nektarios D Giadinis, Vassilios Psychas, Chrysostomos I Dovas
Enzootic nasal adenocarcinoma (ENA) is a contagious neoplasm of sheep and goats, associated with the oncogenic retroviruses enzootic nasal tumor virus (ENTV) 1 and 2, respectively. It appears to be common in countries with substantial small ruminant-production. ENA diagnosis in goats is based on autopsy and histopathology, and there is no real-time PCR method available for ENTV-2 detection. Here, a novel one-tube real-time RT-PCR (RT-qPCR) method for the detection and quantification of ENTV-2 in nasal swabs is presented...
January 2, 2019: Archives of Virology
https://www.readbyqxmd.com/read/30600351/echovirus-plays-a-major-role-in-natural-recombination-in-the-coxsackievirus-b-group
#8
Xufeng Pu, Yongjiang Qian, Yu Yu, Hongxing Shen
Coxsakievirus (CV) B4, CVB5, and CVA9 belong to the species Enterovirus B. These viruses can cause viral encephalitis, aseptic meningitis, pancreatitis, flaccid paralysis, dilated myocarditis, and hand, foot, and mouth disease (HFMD). In order to analyze the evolution of CVB4, CVB5, and CVA9, we analyzed all of the available genome sequences of Enterovirus B (EVB) isolates and found that there were 12 putative recombination events that produced CVB4, 13 putative recombination events that produced CVB5, and 10 putative recombination events that produced CVA9...
January 1, 2019: Archives of Virology
https://www.readbyqxmd.com/read/30600350/complete-genome-sequence-of-a-novel-mitovirus-from-the-wheat-stripe-rust-fungus-puccinia-striiformis
#9
Li Zheng, Jing Zhao, Xiaofei Liang, Hua Zhuang, Tuo Qi, Zhensheng Kang
The complete genome of a novel mycovirus, Puccinia striiformis mitovirus 1 (PsMV1), derived from the wheat stripe rust fungus Puccinia striiformis strain SCSN-10, was sequenced and analyzed. The full-length cDNA sequence is 2496 bp in length with a predicted AU content of 57.65% in the genomic RNA. Sequence analysis indicated that a single large open reading frame (ORF) is present on the positive strand when the fungal mitochondrial genetic code is used. The single ORF encodes a putative RNA-dependent RNA polymerase of 743 amino acids with a molecular mass of 84...
January 1, 2019: Archives of Virology
https://www.readbyqxmd.com/read/30569277/asian-black-bear-ursus-thibetanus-picornavirus-related-to-seal-aquamavirus-a
#10
Haoning Wang, Wen Zhang, Shixiing Yang, Ning Kong, Hai Yu, Hao Zheng, Fei Gao, Wu Tong, Liwei Li, Xiaolong Wang, Xutao Deng, Eric Delwart, Tongling Shan
The complete genome of a bear picornavirus 1 (BePV-1) in the viscera of an Asian black bear (Ursus thibetanus) from China was characterized using viral metagenomics and RT-PCR/Sanger sequencing. The genome of BePV1 is 6703 nt long, contains a type-IV IRES 5'UTR with the '8-like' motif, encodes a 2053-aa-long polyprotein showing a 3-4-4 organization pattern and two 2A genes. BePV-1 showed the highest overall genome nucleotide sequence identity of 71.7% to a picornavirus genome from an Arctic ringed seal (Phoca hispida) from Canada, classified as a member of the species Aquamavirus A, currently the only one in the genus Aquamavirus...
December 20, 2018: Archives of Virology
https://www.readbyqxmd.com/read/30569276/prevalence-of-hiv-1-transmitted-drug-resistance-and-viral-suppression-among-recently-diagnosed-adults-in-s%C3%A3-o-paulo-brazil
#11
Luana Portes Ozorio Coelho, Elaine Monteiro Matsuda, Roberta Schiavon Nogueira, Mônica Jacques de Moraes, Leda Fatima Jamal, José Valdez Ramalho Madruga, Mariza Vono Tancredi, Aline Carralas Queiroz de Leão, Giselle de Faria Romero Soldi, Luís Fernando de Macedo Brígido
HIV-1 transmitted drug resistance (TDR) mutations may reduce the efficacy of antiretroviral therapy (ART), but pre-treatment testing to determine the virus genotype can improve the efficacy of ART. Unfortunately, issues related to cost and logistics of pre-treatment testing limit its use in resource-limited settings. We studied 596 ART-naive individuals who were newly diagnosed from 2014 to 2016 in São Paulo, Brazil, to evaluate TDR and virological outcome after 48 weeks of genotype-guided therapy. One or more TDR (based on the WHO surveillance list) was observed in 10...
December 20, 2018: Archives of Virology
https://www.readbyqxmd.com/read/30564896/isolation-and-characterization-of-duck-adenovirus-3-circulating-in-china
#12
Shaohua Shi, Rongchang Liu, Chunhe Wan, Longfei Cheng, Zhen Chen, Guanghua Fu, Hongmei Chen, Qiuling Fu, Yu Huang
Recently, infectious disease outbreaks characterized by swelling and hemorrhagic liver and kidneys occurred in Muscovy ducklings in China. Four viruses were isolated and identified as adenoviruses by transmission electron microscopy (TEM) and polymerase chain reaction (PCR). Sequence analysis identified the new isolates as duck adenovirus 3 (DAdV-3), species Duck aviadenovirus B. The pathogenicity of the new isolate DAdV-3 FJGT01 was investigated using challenge experiments. The gross lesions in the animal experiment were similar to the clinical lesions observed in the diseased ducks...
December 18, 2018: Archives of Virology
https://www.readbyqxmd.com/read/30560292/characterization-of-a-novel-lytic-myophage-phia8-29-infecting-aeromonas-strains
#13
Yang He, Zhiwei Huang, Xixi Zhang, Zhiqiang Zhang, Mengxin Gong, Xuewei Pan, Dongsheng Wei, Hongjiang Yang
Members of the bacterial genus Aeromonas are important aquatic pathogens that cause severe fish diseases. Here, we characterize a novel lytic phage, Aeromonas virus phiA8-29, isolated from the alimentary tract of a freshwater fish. Transmission electron microscopy showed that phiA8-29 has a long contractile tail and thus can be classified as a member of the family Myoviridae. The phage genome was identified as a double-stranded DNA molecule of 144,974 bp containing 185 open reading frames and nine tRNA-encoding genes...
December 17, 2018: Archives of Virology
https://www.readbyqxmd.com/read/30539262/pathogenicity-of-two-novel-human-origin-h7n9-highly-pathogenic-avian-influenza-viruses-in-chickens-and-ducks
#14
Taichiro Tanikawa, Yuko Uchida, Nobuhiro Takemae, Ryota Tsunekuni, Junki Mine, Ming-Tsan Liu, Ji-Rong Yang, Masayuki Shirakura, Shinji Watanabe, Takato Odagiri, Takehiko Saito
Human infection by low-pathogenic avian influenza viruses of the H7N9 subtype was first reported in March 2013 in China. Subsequently, these viruses caused five outbreaks through September 2017. In the fifth outbreak, H7N9 virus possessing a multiple basic amino acid insertion in the cleavage site of hemagglutinin emerged and caused 4% of all human infections in that period. To date, H7N9 highly pathogenic avian influenza viruses (HPAIVs) have been isolated from poultry, mostly chickens, as well as the environment...
December 11, 2018: Archives of Virology
https://www.readbyqxmd.com/read/30535807/development-of-a-sensitive-and-reliable-reverse-transcription-droplet-digital-pcr-assay-for-the-detection-of-citrus-yellow-vein-clearing-virus
#15
Yingjie Liu, Yingli Wang, Qin Wang, Yanhui Zhang, Wanxia Shen, Ruhui Li, Mengji Cao, Lei Chen, Xue Li, Changyong Zhou, Yan Zhou
In 2009, a new viral disease of citrus caused by citrus yellow vein clearing virus (CYVCV) was first discovered in China. CYVCV is considered to be the most serious pathogen affecting lemon production. In this study, a sensitive and reliable reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay was developed to detect and quantify CYVCV without references. The specificity of the assay was demonstrated by its failure to amplify other relevant citrus viruses. The quantitative linearity, sensitivity and accuracy of RT-ddPCR for detecting CYVCV were compared to those of real-time RT-PCR...
December 11, 2018: Archives of Virology
https://www.readbyqxmd.com/read/30535806/first-report-of-a-novel-alphapartitivirus-in-the-basidiomycete-rhizoctonia-oryzae-sativae
#16
Subha Das, Sampa Das
Rhizoctonia oryzae-sativae is a soil-borne basidiomycete fungus that causes aggregate sheath spot disease on rice worldwide. Here, we report the complete genome sequence of a partitivirus designated as Rhizoctonia oryzae-sativae partitivirus 1 (RosPV1) infecting this fungus. The genome of RosPV1 consists of two double-stranded RNA (dsRNA) segments. The larger segment, designated as dsRNA-1 (1,961 bp), contains a single open reading frame (ORF) that encodes a putative polypeptide with a conserved RNA-dependent RNA polymerase (RdRp) domain...
December 11, 2018: Archives of Virology
https://www.readbyqxmd.com/read/30535562/complete-genome-sequence-of-a-novel-bacteriophage-pbkp05-infecting-klebsiella-pneumoniae
#17
Hyun Keun Oh, Kyoungeun Cha, Yoon Jung Hwang, Jaehak Cho, Yunyeol Jo, Heejoon Myung
An increasing number of Klebsiella pneumoniae isolates have been found to be multi-drug resistant. A novel bacteriophage, PBKP05, which infects K. pneumoniae, was isolated and characterized. It has a linear double-stranded DNA genome of 30,240 base pairs in length. Its G+C content is 53%, and 47 putative open reading frames are functionally annotated. This phage can be a candidate material for phage therapy.
December 10, 2018: Archives of Virology
https://www.readbyqxmd.com/read/30536126/correction-to-association-of-cxcr2-genotype-variations-with-hcv-clearance-in-a-chinese-population
#18
Feng Zang, Ming Yue, Lingyun Zhuo, Jingjing Wu, Mei Liu, Yinan Yao, Jie Wang, Yue Feng, Xueshan Xia, Peng Huang, Rongbin Yu
Unfortunately, the funding statement was published with error in original publication and is corrected here.
December 8, 2018: Archives of Virology
https://www.readbyqxmd.com/read/30535526/genomic-characterization-phylogenetic-position-and-in-situ-localization-of-a-novel-putative-mononegavirus-in-lepeophtheirus-salmonis
#19
Arnfinn Lodden Økland, Are Nylund, Aina-Cathrine Øvergård, Renate Hvidsten Skoge, Heidi Kongshaug
The complete genome sequence of a novel mononegavirus, Lepeophtheirus salmonis negative-stranded RNA virus 1 (LsNSRV-1), obtained from a salmonid ectoparasite, Lepeophtheirus salmonis was determined. The viral genome contains five open reading frames encoding three unknown proteins (ORF I, II and III), a putative glycoprotein (G), and a large (L) protein. Phylogenetic analysis placed LsNSRV-1 in the recently established mononegaviral family Artoviridae. LsNSRV-1 showed a prevalence of around 97% and was detected in all L...
December 7, 2018: Archives of Virology
https://www.readbyqxmd.com/read/30523430/characterization-of-the-lytic-archaeal-virus-drs3-infecting-methanobacterium-formicicum
#20
Sandro Wolf, Martin A Fischer, Anne Kupczok, Jochen Reetz, Tobias Kern, Ruth A Schmitz, Michael Rother
Viruses are ubiquitous in the biosphere and greatly affect the hosts they infect. It is generally accepted that members of every microbial taxon are susceptible to at least one virus, and a plethora of bacterial viruses are known. In contrast, knowledge of the archaeal virosphere is still limited. Here, a novel lytic archaeal virus is described, designated "Drs3", as well as its host, Methanobacterium formicicum strain Khl10. This hydrogenotrophic methanogenic archaeon and its virus were isolated from the anaerobic digester of an experimental biogas plant in Germany...
December 6, 2018: Archives of Virology
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