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Archives of Virology

Katherine A Sayler, Kuttichantran Subramaniam, Jessica M Jacob, Julia C Loeb, William F Craft, Lisa L Farina, Nicole I Stacy, Nissin Moussatche, Laurie Cook, John A Lednicky, Samantha M Wisely, Thomas B Waltzek
Infections caused by mule deerpox virus (MDPV) have been sporadically reported in North American cervids. White-tailed deer (Odocoileus virginianus) fawns from a farm located in South Central Florida presented with ulcerative and crusting lesions on the coronary band as well as the mucocutaneous tissues of the head. Evaluation of the crusted skin lesions was undertaken using microscopic pathology and molecular techniques. A crusted skin sample was processed for virus isolation in four mammalian cell lines. The resulting isolate was characterized by negative staining electron microscopy and deep sequencing...
September 21, 2018: Archives of Virology
Domonkos Sváb, Linda Falgenhauer, Manfred Rohde, Trinad Chakraborty, István Tóth
The genome sequence of a novel virulent bacteriophage, named " C130_2", that is morphologically a member of the family Myoviridae is reported. The 41,775-base-pair double-stranded DNA genome of C130_2 contains 59 ORFs but exhibits overall low sequence similarity to bacteriophage genomes for which sequences are publicly available. Phylogenetic analysis indicated that C130_2 represents a new phage type. C130_2 could be propagated well on enterohemorrhagic Escherichia coli (EHEC) O157:H7 and other pathogenic E...
September 20, 2018: Archives of Virology
Keita Noguchi, Ryusei Kuwata, Hiroshi Shimoda, Tetsuya Mizutani, Eiichi Hondo, Ken Maeda
In a comprehensive research project on bat viruses, we successfully isolated a novel herpesvirus from the spleen of a greater horseshoe bat (Rhinolophus ferrumequinum) in Japan using a cell line established from the kidney of the same bat. This herpesvirus was a novel gammaherpesvirus (Rhinolophus gammaherpesvirus 1; RGHV-1), which belonged to the genus Percavirus. The whole RGHV-1 genome (147,790 bp) showed that 12 of the 84 genes predicted to contain open reading frames did not show any homology to those of other herpesviruses...
September 19, 2018: Archives of Virology
Wei Li, Yunlei Xia, Haotian Zhang, Xing Zhang, Huaigu Chen
A Victorivirus was detected in isolate F16176 of the fungus Fusarium asiaticum, the causal agent of Fusarium head blight in China. The full genome sequence of the virus was sequenced and characterized. The complete cDNA sequence is 5,281 nucleotides long with 64.2% G + C content and contains two open reading frames (ORFs) that overlap at the pentanucleotide UAAUG. The two ORFs are predicted to encode coat protein (CP) and RNA-dependent RNA polymerase (RdRp), which are conserved among the dsRNA mycoviruses of the genus Victorivirus...
September 19, 2018: Archives of Virology
Xingqi Zou, Yuanyuan Zhu, Huifang Bao, Xiaoyu Guo, Pu Sun, Zaixin Liu, Peter W Mason, Lu Xu, Cui Li, Qianyi Zhang, Qin Wang, Hongfei Zhu, Qizu Zhao
Foot-and-mouth disease virus (FMDV) exhibits high mutation rates during replication. In this study, an isolate of FMDV serotype Asia-1 was serially passaged in a BHK-21 cell monolayer and then adapted to serum-free BHK-21 cell suspension culture to produce a seed virus for production of an inactivated vaccine. Analysis of the sequence encoding the structural proteins of the virus at various passages showed the presence of overlapping peaks in sequencing electropherograms after nucleotide 619 of VP1 in viruses recovered from the fourth passage in suspension culture, suggesting the possible introduction of an insertion or deletion into this portion of the viral genome of our seed virus stock...
September 19, 2018: Archives of Virology
Thiago Marques Costa, Rosana Blawid, Miguel A Aranda, Débora Maria Sansini Freitas, Genira Pereira Andrade, Alice Kazuko Inoue-Nagata, Tatsuya Nagata
Melon plants with severe yellowing symptoms from in Brazil were analyzed by high-throughput sequencing. Sequences homologous to the genome of the polerovirus cucurbit aphid-borne yellows virus (CABYV) were frequently retrieved. Two draft CABYV genomes were assembled from two pooled melon samples that contained an identical putative recombinant fragment in the 3' region with an unknown polerovirus. The complete genomes of these isolates revealed by Sanger sequencing share 96.8% nucleotide identity, while both sequences share 73...
September 19, 2018: Archives of Virology
Yibao Chen, Lan Yang, Erchao Sun, Jiaoyang Song, Bin Wu
A novel virulent bacteriophage, vB_BbrM_PHB04, infecting Bordetella bronchiseptica was isolated from wastewater collected at a swine farm in China. Phage vB_BbrM_PHB04 exhibited growth over a wide range of temperature and pH conditions and showed different efficiency of plating values and lytic spectra within the same strains at 25 °C and 37 °C. High-throughput sequencing revealed that vB_BbrM_PHB04 has a linear double-stranded DNA genome with 124 putative open reading frames. Overall, the genome of vB_BbrM_PHB04 showed very low similarity (the highest nucleotide identity 82%, 1% coverage) to other phage sequences in the GenBank database...
September 18, 2018: Archives of Virology
Wei-Feng Tang, De-Guang Kong, Yuan-Hong Wang, Man-Qing Liu, Quan Hu
Hepatitis E virus (HEV) is an emergent virus of global importance. Previous studies of HEV infection in China mainly focused on the rural areas. This work aims to study the epidemiology of HEV in a large urban environment. With a registered population of 10 million, the dense city of Wuhan presents itself as a prime opportunity to better understand this emergent virus. The epidemiological data from 2011 to 2016 were analyzed. A cross-sectional study on the seroprevalence of anti-HEV IgG was conducted among the general population (age range 0-59) in 2013...
September 18, 2018: Archives of Virology
Hui Wang, Yan Liu, Wenwen Liu, Mengji Cao, Xifeng Wang
A novel iflavirus was discovered in Psammotettix alienus by RNA sequencing. The virus genome has 10,826 nucleotides (nt) excluding the poly A tail, encodes a 3187-amino-acid polyprotein, and is tentatively named "Psammotettix alienus iflavirus 1" (PaIV1). PaIV1 has a similar genomic structure, conserved motifs, and a close phylogenetic relationship to members of the genus Iflavirus, but it shows only 41.9-55.2% sequence identity in the full genome and 16.2-49% in the deduced polyprotein. Thus, we suggest that it is a new member of the genus Iflavirus, family Iflaviridae...
September 18, 2018: Archives of Virology
Vasily A Evseenko, Natalia P Kolosova, Anderi S Gudymo, Semion V Maltsev, Julia A Bulanovich, Nataliya I Goncharova, Natalia V Danilchenko, Vasiliy Y Marchenko, Ivan M Susloparov, Alexander B Ryzhikov
Cyclamen europaeum tubers extract (CTE) with concentration commonly used for human rhinosinusitis treatment was tested as mucosal adjuvant in experimental intranasal immunization of guinea pigs with concentrated commercially available influenza trivalent vaccine and subsequent infection with influenza strain A/California/04/2009 H1N1pdm. Dual intranasal immunization with vaccine compound consisting of 7.5 µg of each hemagglutinin and 500 µg of CTE in 50 µl induced reciprocal GMT on day 21 after immunization 40 (5-640) against H1N1pdm; 43...
September 17, 2018: Archives of Virology
Mengji Cao, Ping Li, Song Zhang, Fangyun Yang, Yan Zhou, Xuefeng Wang, Ruhui Li, Zhongan Li
A novel positive-strand RNA virus infecting citrus with the tentative name "citrus leaf blotch virus 2" (CLBV-2), was identified in the present work. The complete genome sequence of CLBV-2 comprises 8,697 nucleotides (nt) excluding a poly(A) tail and three open reading frames (ORFs), showing the highest nucleotide sequence identity with the Actinidia strain (JN983456) of citrus leaf blotch virus (CLBV). The putative movement protein (ORF2), coat protein (ORF3), and 3' untranslated region (UTR) shared high sequence similarity with those of the extant CLBV isolates...
September 17, 2018: Archives of Virology
Wataru Mitsuhashi, Sachiko Shimura, Kazuhisa Miyamoto, Takafumi N Sugimoto
Oral inoculation of entomopoxvirus spindles, microstructures composed of fusolin protein, causes disruption of the peritrophic matrix (PM), a physical barrier against microbe infection, in the insect midgut. Although the atomic structure of fusolin has been determined, little has been directly elucidated of the mechanism of disruption of the PM. In the present study, we first performed an immunohistochemical examination to determine whether fusolin acts on the PM directly or indirectly in the midgut of Bombyx mori larvae that were inoculated with spindles of Anomala cuprea entomopoxvirus...
September 17, 2018: Archives of Virology
Yan Wang, Shixing Yang, Dawei Liu, Chenglin Zhou, Wang Li, Yuan Lin, Xiaochun Wang, Quan Shen, Hua Wang, Chuang Li, Minghui Zong, Yuzhu Ding, Qianben Song, Xutao Deng, Dunwu Qi, Wen Zhang, Eric Delwart
The red-crowned crane is one of the rarest crane species, and its population is decreasing due to loss of habitat, poisoning, and infections. Using a viral metagenomics approach, we analyzed the virome of feces from wild and captive red-crowned cranes, which were pooled separately. Vertebrate viruses belonging to the families Picornaviridae, Parvoviridae, Circoviridae, and Caliciviridae were detected. Among the members of the family Picornaviridae, we found three that appear to represent new genera. Six nearly complete genomes from members of the family Parvoviridae were also obtained, including four new members of the proposed genus "Chapparvovirus", and two members of the genus Aveparvovirus...
September 17, 2018: Archives of Virology
Cécile Richet, Simona Kraberger, Denis Filloux, Pauline Bernardo, Gordon W Harkins, Darren P Martin, Philippe Roumagnac, Arvind Varsani
Advances in molecular techniques used in viral metagenomics coupled with high throughput sequencing is rapidly expanding our knowledge of plant-associated virus diversity. Applying such approaches, we have identified five novel circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA viruses from Poaceae and Apiaceae plant from South Africa and New Zealand. These viruses have a simple genomic organization, including two open reading frames that likely encode a Rep and a capsid protein (CP), a conserved nonanucleotide motif on the apex of a putative stem loop structure, and conserved rolling-circle replication and helicase motifs within their likely Rep: all suggesting that they replicate through rolling-circle replication...
September 15, 2018: Archives of Virology
Katsuhiko Ito, Takeshi Fujii, Takeshi Yokoyama, Keiko Kadono-Okuda
Bombyx mori bidensovirus (BmBDV) is a pathogen that replicates only in the midgut columnar cells of silkworms, causing fatal disease. Resistance to BmBDV, which does not depend on the viral dose, is determined by a single gene, nsd-2 (resistance gene). Previously, we identified nsd-2 by positional cloning using B. mori genome information and found that this gene encodes a putative amino acid transporter that may function as a receptor for BmBDV. In this study, to understand the relationship between BmBDV and the putative virus receptor, we performed expression analysis of +nsd-2 (allele of nsd-2; susceptibility gene) after virus infection...
September 15, 2018: Archives of Virology
Samuel Namonyo, Maina Wagacha, Solomon Maina, Lillian Wambua, Morris Agaba
This project sought to investigate the domestic caprid rumen virome by developing a robust viral DNA isolation and enrichment protocol (utilizing membrane filtration, ultra-centrifugation, overnight PEG treatment and nuclease treatment) and using RSD-PCR and high throughput sequencing (HTS) techniques. 3.53% of the reads obtained were analogous to those of viruses denoting Siphoviridae, Myoviridae, Podoviridae, Mimiviridae, Microviridae, Poxviridae, Tectiviridae and Marseillevirus. Most of the sequenced reads from the rumen were similar to those of phages, which are critical in maintaining the rumen microbial populations under its carrying capacity...
September 15, 2018: Archives of Virology
Santiago Mirazo, Cecilia D Albora, Diana Quintero Gil, Karina Cabrera, Natalia Ramos, Sergio Ordúz, Juan Arbiza
Hepatitis E virus (HEV) infection involving zoonotic genotypes is a public health problem in high-income and non-endemic developing countries. Herein we report the detection of a human genotype 1 (HEV-1) strain infecting a domestic pig, which is not considered a natural reservoir of this genotype. Viral load was quantified in stool by Real-Time qPCR and sequence analyses were performed. Infectivity of the HEV-1 strain was assesed by in vitro isolation in A549 cell line. Results suggest that certain epidemiological settings might favour accidental spillover infection and thus influence the host range restriction of HEV...
September 15, 2018: Archives of Virology
Lan Li, Xiaoning Tian, Jin Chen, Pengcheng Li, Qisheng Zheng, Jibo Hou
Porcine reproductive and respiratory syndrome virus (PRRSV) is a pathogen that severely disrupts swine production. Despite sustained efforts, the disease is still endemic, with high mortality and morbidity. New antiviral strategies to control PRRSV are needed. Griffithsin, a red algal lectin, has potent antiviral effect on several human enveloped viruses, but this effect has not been demonstrated on PRRSV. Here, we first tested the in vitro antiviral activity of Griffithsin against PRRSV. Griffithsin exerted strong saccharide-dependent antiviral activity against PRRSV, probably through interactions with glycans on the surface of PRRSV that interfered with virus entry...
September 15, 2018: Archives of Virology
Xiaoqin Peng, Shexiang Gan, Xiaoting Zhang, Yingying Zhai, Lina Cai, Fei Yan, Songbai Zhang
A novel double-stranded RNA (dsRNA) virus, which was provisionally named Arthrocladiella mougeotii endornavirus (AmEV), was isolated from Arthrocladiella mougeotii, the phytopathogenic fungus infecting Lycium chinense in Beijing, China. The genome of AmEV is 11,683 nucleotides in length with a 5' and 3' non-coding region of 16 and 50 nt, respectively, as well as a single 11,617-nt long open reading frame potentially encoding a putative protein of 3,871 amino acids with conserved Helicase and RNA-dependent RNA polymerase (RdRp) domains...
September 15, 2018: Archives of Virology
Min Sun Kim, Ki Hong Kim
Hypoxia-inducible factor-1 (HIF-1) is a heterodimer of HIF-1α and HIF-1β, and its key role in the regulation of cellular responses to hypoxia has been well-demonstrated. The participation of HIF-1α in apoptosis has been reported in mammals, however, a little information is available on the role of HIF-1α in the progression of apoptosis in fish. In this study, to know the role of HIF-1α in the apoptosis of fish cells, we produced HIF-1α knockout Epithelioma papulosum cyprini (EPC) cells using a CRISPR/Cas9 vector, and a single cell clone showing a heterozygous insertion/deletion (indel) mutation (one nucleotide insertion and one nucleotide deletion in HIF-1α gene) was chosen for further experiments...
September 15, 2018: Archives of Virology
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