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Mycopathologia

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https://www.readbyqxmd.com/read/29445920/phylogenetic-diversity-and-in-vitro-susceptibility-profiles-of-human-pathogenic-members-of-the-fusarium-fujikuroi-species-complex-isolated-from-south-india
#1
Ananya Tupaki-Sreepurna, Vijayakishore Thanneru, Sangeetha Natarajan, Savitri Sharma, Anjana Gopi, Murugan Sundaram, Anupma Jyoti Kindo
Availability of molecular methods, gene sequencing, and phylogenetic species recognition have led to rare fungi being recognized as opportunistic pathogens. Fungal keratitis and onychomycosis are fairly common mycoses in the tropics, especially among outdoor workers and enthusiasts. The frequently isolated etiological agents belong to genera Candida, Aspergillus, and Fusarium. Within the genus Fusarium, known to be recalcitrant to prolonged antifungal treatment and associated with poor outcome, members of the Fusarium solani species complex are reported to be most common, followed by members of the Fusarium oxysporum SC and the Fusarium fujikuroi SC (FFSC)...
February 14, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29383575/antifungal-susceptibility-morphological-and-molecular-characterization-of-lasiodiplodia-theobromae-isolated-from-a-patient-with-keratitis
#2
Priscila Dallé da Rosa, Claudete Locatelli, Karla Scheid, Diane Marinho, Lúcia Kliemann, Alexandre Fuentefria, Luciano Zubaran Goldani
Lasiodiplodia theobromae is a rare ocular pathogen. We report a patient with fungal keratitis caused by L. theobromae. The patient was a 75-year-old male, a farmer with diabetes type II, and no previous history of ocular trauma. Histopathology analysis revealed the presence fungi invading Descemet's membrane of the cornea. The fungus was characterized by septate, highly bulged fungal filaments involving full corneal thickness in the corresponding histopathology specimens. A dematiaceous mold was isolated and initally identified as L...
January 30, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29383574/a-fatal-case-of-candida-auris-and-candida-tropicalis-candidemia-in-neutropenic-patient
#3
Ratna Mohd Tap, Teck Choon Lim, Nur Amalina Kamarudin, Stephanie Jane Ginsapu, Mohd Fuat Abd Razak, Norazah Ahmad, Fairuz Amran
We report a fatal case of Candida auris that was involved in mixed candidemia with Candida tropicalis, isolated from the blood of a neutropenic patient. Identification of both isolates was confirmed by amplification and sequencing of internal transcribed spacer and D1/D2 domain of large subunit in rRNA gene. Antifungal susceptibility test by E-test method revealed that C. auris was resistant to amphotericin B, anidulafungin, caspofungin, fluconazole, itraconazole and voriconazole. On the other hand, C. tropicalis was sensitive to all antifungal tested...
January 30, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29380188/comparative-study-of-the-effects-of-fluconazole-and-voriconazole-on-candida-glabrata-candida-parapsilosis-and-candida-rugosa-biofilms
#4
Priya Madhavan, Farida Jamal, Chong Pei Pei, Fauziah Othman, Arunkumar Karunanidhi, Kee Peng Ng
Infections by non-albicans Candida species are a life-threatening condition, and formation of biofilms can lead to treatment failure in a clinical setting. This study was aimed to demonstrate the in vitro antibiofilm activity of fluconazole (FLU) and voriconazole (VOR) against C. glabrata, C. parapsilosis and C. rugosa with diverse antifungal susceptibilities to FLU and VOR. The antibiofilm activities of FLU and VOR in the form of suspension as well as pre-coatings were assessed by XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction assay...
January 29, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29380187/correction-to-resistance-mechanism-in-a-terbinafine-resistant-strain-of-microsporum-canis
#5
Rui Kano, Yun-Hsia Hsiao, Hock Siew Han, Charles Chen, Atsuhiko Hasegawa, Hiroshi Kamata
In the initial online publication, the name of author Hock Siew Han was given incorrectly as Han Hock Siew. The original article has been corrected.
January 29, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29380186/the-effect-of-some-natural-essential-oils-against-bovine-mastitis-caused-by-prototheca-zopfii-isolates-in-vitro
#6
Barbara Grzesiak, Barbara Kołodziej, Anna Głowacka, Henryk Krukowski
The aim of the study was to evaluate the effect of essential oils obtained from Thymus vulgaris L., Origanum vulgare L., Origanum majerana L., Mentha × piperita L. and Allium ursinum L. against Prototheca zopfii strains that cause inflammation of the udder (mastitis) in cows. The study was conducted on ten strains derived from milk samples. The microdilution method was used to determine the sensitivity of P. zopfii strains to the studied essential oils, and the disk diffusion method was used to determine the sensitivity to antifungal chemotherapeutics...
January 27, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29374798/exophiala-dermatitidis-endophthalmitis-case-report-and-literature-review
#7
Mónika Homa, Palanisamy Manikandan, Veerappan Saravanan, Rajaraman Revathi, Raghavan Anita, Venkatapathy Narendran, Kanesan Panneerselvam, Coimbatore Subramanian Shobana, Mohammed Al Aidarous, László Galgóczy, Csaba Vágvölgyi, Tamás Papp, László Kredics
We report a case of a 59-year-old male patient with a postoperative fungal infection of the left eye. A dark-pigmented yeast, Exophiala dermatitidis (previously known as Wangiella dermatitidis), was identified from the culture of the biopsy taken from the posterior capsule. The infection was successfully eradicated by a combination of surgical and medical (i.e., voriconazole and fluconazole) treatment. This is the first report of successfully treated E. dermatitidis endophthalmitis, which demonstrates that a prompt and aggressive antifungal therapy combined with surgical intervention is necessary to prevent vision loss in cases of endophthalmitis due to Exophiala species...
January 27, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29356937/a-retrospective-assessment-of-four-antigen-assays-for-the-detection-of-invasive-candidiasis-among-high-risk-hospitalized-patients
#8
Barbara Hartl, Iris Zeller, Angelika Manhart, Brigitte Selitsch, Cornelia Lass-Flörl, Birgit Willinger
Because of their high mortality rates and non-specific symptoms, invasive Candida infections pose a huge diagnostic and therapeutic challenge. In this study, we evaluated the three mannan antigen assays Platelia, Platelia Plus and Serion, and the (1-3)-β-D-glucan assay Fungitell in a group of high-risk (hematological and surgical) patients. Test results of 305 patients hospitalized at the Vienna General Hospital and the University Hospital of Innsbruck were retrospectively analyzed. We assessed the test accuracy by means of descriptive statistics...
January 22, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29349620/fatal-talaromyces-marneffei-infection-in-a-patient-with-autoimmune-hepatitis
#9
Sally C Y Wong, Siddharth Sridhar, Antonio H Y Ngan, Jonathan H K Chen, Rosana W S Poon, Susanna K P Lau, Patrick C Y Woo
Talaromyces marneffei, previously known as Penicillium marneffei, is the most important pathogenic thermally dimorphic fungus causing systemic mycosis in Southeast Asia. Traditionally, T. marneffei infection in human was mainly associated with acquired immunodeficiency syndrome caused by HIV infection. In recent years, there has been an increasing number of T. marneffei infections reported in non-HIV-infected patients with other immunocompromised conditions, including autoantibodies against interferon-gamma, systemic lupus erythematosis, solid organ transplantation, Job's syndrome, hematological malignancies, and use of novel targeted therapies...
January 18, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29344845/two-cases-of-dermatophytic-granuloma-successfully-treated-with-terbinafine
#10
Xiangxi Wang, Yong Yang, Ruoyu Li, Jin Yu
Dermatophytic granuloma, also called Majocchi's granuloma (MG), is an uncommon infection of the dermis and subcutaneous tissues that can occur in both healthy and immunosuppressed hosts. We present two cases of MG with different clinical features. Both patients had satisfactory relief after treatment with terbinafine.
January 17, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29340910/resistance-mechanism-in-a-terbinafine-resistant-strain-of-microsporum-canis
#11
Rui Kano, Yun-Hsia Hsiao, Han Hock Siew, Charles Chen, Atsuhiko Hasegawa, Hiroshi Kamata
To clarify the terbinafine (TRF) resistance mechanism in a TRF-resistant strain of Microsporum canis, the expression of the pleiotropic drug resistance (PDR1), multidrug resistance (MDR1), MDR2 and MDR4 genes were investigated by real-time quantitative PCR (RT-qPCR) analysis, given the known interaction of the corresponding proteins with antifungals and with the efflux blocker FK506. The expression of the PDR1, MDR1, MDR2 and MDR4 genes was 2-4 times higher in the TRF-resistant strain grown in the presence of 0...
January 16, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29340909/liposomal-amphotericin-b-as-monotherapy-in-relapsed-coccidioidal-meningitis
#12
Ethan R Stewart, Matthew L Eldridge, Ian McHardy, Stuart H Cohen, George R Thompson
Coccidioidal meningitis remains a difficult clinical problem, and despite life-long therapy with triazole antifungals, relapses of disease and medication intolerance occur necessitating salvage treatment. We report two patients with recurrent coccidioidal meningitis who improved following a 2-week course of liposomal amphotericin B monotherapy and discuss potential advantages of this treatment option.
January 16, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29335843/study-on-the-clinical-features-and-prognosis-of-penicilliosis-marneffei-without-human-immunodeficiency-virus-infection
#13
Ying-Ge Wang, Jin-Mei Cheng, Hai-Bo Ding, Xi Lin, Guo-Hao Chen, Mei Zhou, Sheng-Nan Ye
OBJECTIVE: To improve the diagnosis and treatment of Penicilliosis marneffei without human immunodeficiency virus infection. METHODS: Analyze and review the clinical features, diagnosis and treatment of six cases of P. marneffei without human immunodeficiency virus infection at The First Affiliated Hospital of Fujian Medical University. RESULTS: Two cases were diagnosed in the ENT Department, three cases in the respiratory department and one case in the dermatological department...
January 15, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29249044/endogenous-fusarium-endophthalmitis-during-treatment-for-acute-myeloid-leukemia-successfully-treated-with-25-gauge-vitrectomy-and-antifungal-medications
#14
Masaaki Yoshida, Naoki Kiyota, Kazuichi Maruyama, Hiroshi Kunikata, Masahiro Toyokawa, Shigehiro Hagiwara, Koichi Makimura, Naomi Sato, Shinji Taniuchi, Toru Nakazawa
Endogenous fungal endophthalmitis (EFE) caused by disseminated fusariosis is a rare condition that generally has a poor outcome, even with intensive therapy. Here, we describe a case in which this type of EFE was diagnosed with vitreous sampling and was successfully treated with 25-gauge vitrectomy and antifungals, including liposomal amphotericin B and voriconazole. A 16-year-old male patient undergoing treatment for acute myeloid leukemia complained of eye pain and blurred vision in his right eye. Treatment was initiated for a vitreous opacity, possibly associated with herpetic retinitis, but the patient worsened and he was referred to us...
December 16, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29222709/successful-treatment-of-canine-sporotrichosis-with-terbinafine-case-reports-and-literature-review
#15
Paula Gonçalves Viana, Anna Barreto Fernandes Figueiredo, Isabella Dib Ferreira Gremião, Luisa Helena Monteiro de Miranda, Isabela Maria da Silva Antonio, Jéssica Sepulveda Boechat, Ana Caroline de Sá Machado, Manoel Marques Evangelista de Oliveira, Sandro Antonio Pereira
Sporotrichosis occurs worldwide, and the metropolitan region of Rio de Janeiro, Brazil, is a main endemic area, with a large number of human and animal cases in the last 19 years. This mycosis is more frequently described in cats rather than in dogs. There are a limited number of oral antifungal agents for the treatment of sporotrichosis in animals. In this context, the effectiveness of terbinafine in the treatment of sporotrichosis in humans, as well as the promising results of in vitro susceptibility tests, inspired us to use this drug in the therapy of this mycosis in dogs...
December 8, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29214455/cryptococcus-gattii-infection-presenting-as-an-aggressive-lung-mass
#16
Shuwei Zheng, Thuan Tong Tan, Jaime Mei Fong Chien
Cryptococcus gattii is an endemic fungus predominantly isolated in the tropical and subtropical regions, causing predominantly pulmonary disease with a predilection for the central nervous system. Herein, we report a case of rapidly progressing C. gattii pneumonia in an immune-deficient but virologically suppressed host with underlying human immunodeficiency viral (HIV) infection, exhibiting various fungal morphologies from bronchoalveolar lavage (BAL) cytological specimens. A 51-year-old Chinese male with known HIV disease was admitted to the Singapore General Hospital for evaluation of functional decline, febrile episodes, and a left hilar mass on chest radiograph...
December 6, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29196924/cutaneotrichosporon-trichosporon-debeurmannianum-a-rare-yeast-isolated-from-blood-and-urine-samples
#17
Reema Nath, Pallabi Sargiary, Biswajyoti Borkakoty, Pratap Parida
Cutaneotrichosporon (Trichosporon) debeurmannianum is a rarely isolated yeast from clinical samples. Nine isolates of this yeast were identified from clinical samples within a period of 3 years from June 2012 to May 2015. These isolates were from blood and urine samples sent to a clinical mycology laboratory of a tertiary care hospital in Assam, North East India. Clinically, the patients were diagnosed as septicemia and urinary tract infection. The age of the patients ranged from 2 to 50 years. Identification was made by sequencing the ITS region of ribosomal RNA gene...
December 1, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29196923/mixed-infection-of-respiratory-tract-in-a-dog-caused-by-rhodotorula-mucilaginosa-and-trichosporon-jirovecii-a-case-report
#18
Małgorzata J Biegańska, Magdalena Rzewuska, Iwona Dąbrowska, Bożena Malewska-Biel, Magdalena Ostrzeszewicz, Bożena Dworecka-Kaszak
This report describes the isolation of two environmental fungi: Rhodotorula mucilaginosa and Trichosporon jirovecii accompanied by Pseudomonas aeruginosa and Escherichia coli from a dog with bronchotracheitis. All microorganisms were isolated routinely from a mucopurulent discharge, obtained during bronchoscopy from laryngotracheal area. The initial identification of yeasts was confirmed by API Candida and by molecular analysis of internal transcribed spacer region. Administered antimicrobial treatment with Marbofloxacin and Canizol has brought the improvement in the dogs' health status...
December 1, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29196922/nosocomial-candiduria-in-the-elderly-microbiological-diagnosis
#19
Lidia García-Agudo, Manuel Rodríguez-Iglesias, Rafael Carranza-González
Candiduria is associated with high morbidity, mortality, and long hospitalization, involving high costs for the healthcare system. The use of increasingly aggressive treatments has prolonged the lives of patients susceptible to candiduria, namely the immunosuppressed, the premature, and the elderly. Our objective was to evaluate the incidence of nosocomial candiduria and the implicated species in hospitalized patients aged over 80 years old from three Spanish centers during 2012 and 2013. Urine samples received from these patients were cultured and analyzed by flow cytometry in search of leukocyturia, hematuria, proteinuria, and microbial nitrate reductase activity...
December 1, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29168073/fungus-specific-cd4-t-cells-as-specific-sensors-for-identification-of-pulmonary-fungal-infections
#20
Alexander Scheffold, Carsten Schwarz, Petra Bacher
Patients with cystic fibrosis (CF) suffer from chronic lung infections, caused by bacterial, viral or fungal pathogens, which determine morbidity and mortality. The contribution of individual pathogens to chronic disease and acute lung exacerbations is often difficult to determine due to the complex composition of the lung microbiome in CF. In particular, the relevance of fungal pathogens in CF airways remains poorly understood due to limitations of current diagnostics to identify the presence of fungal pathogens and to resolve the individual host-pathogen interaction status...
November 22, 2017: Mycopathologia
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