journal
MENU ▼
Read by QxMD icon Read
search

Journal of Clinical Microbiology

journal
https://www.readbyqxmd.com/read/29769281/a-comparison-of-the-xpert-flu-rsv-xc-and-xpress-flu-rsv-assays
#1
Elena B Popowitch, Melissa B Miller
Molecular diagnostics for influenza and respiratory syncytial virus (RSV) have become commonplace and a variety of tests and systems have been FDA-cleared for use in the United States. We performed a retrospective study to compare the Cepheid Xpress Flu/RSV assay to the Xpert Flu/RSV XC assay, using laboratory-developed tests (LDTs) as the reference method. The Xpress assay was 100% accurate as compared to the LDTs, while the Xpert Flu/RSV XC was 96.0% accurate. The Xpress test was determined to be faster and more sensitive than the XC...
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29769280/molecular-diagnosis-of-bacterial-vaginosis-an-update
#2
Jenell S Coleman, Charlotte A Gaydos
Bacterial vaginosis (BV) is the most common cause of vaginal discharge in reproductive age women. BV has been associated with poor reproductive outcomes like preterm delivery, acquisition of sexually transmitted infections including HIV, and pelvic inflammatory disease. BV represents acquisition of a diverse community of anaerobic and facultative bacteria and a reduction in lactobacilli. It can be diagnosed using several tests ranging from clinical indicators, point-of-care tests, and molecular assays. Molecular technologies are objective, able to detect fastidious bacteria, allow quantitation, and are ideal for self-collected vaginal swabs...
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29769279/microbial-interactions-in-the-cystic-fibrosis-airway
#3
Ann M Granchelli, Frederick R Adler, Ruth H Keogh, Christiana Kartsonaki, David R Cox, Theodore G Liou
Interactions in the airway ecology of cystic fibrosis may alter organism persistence and clinical outcomes. Better understanding of such interactions could guide clinical decisions. We fitted logistic regression models using generalized estimating equations to longitudinal two-year patient cohorts in the Cystic Fibrosis Foundation Patient Registry, 2003-2011 to study associations between airway organisms present in each calendar year and their presence in the subsequent year. Models were adjusted for clinical characteristics and multiple observations per patient and tested for sensitivity to cystic fibrosis-specific treatments...
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29769278/a-concise-review-of-the-epidemiology-and-diagnostics-of-rickettsioses-rickettsia-and-orientia-spp
#4
Mohammad Yazid Abdad, Rita Abou Abdallah, Pierre-Edouard Fournier, John Stenos, Shawn Vasoo
Rickettsioses are globally distributed and caused by the family Rickettsiaceae , which comprise a diverse and expanding list. These include two genera: Rickettsia and Orientia Serology has been traditionally the main-stay of diagnosis, although this has been limited by cross-reactions among closely related members and diminished sensitivity/utility in the acute phase of illness. Other techniques such as nucleic acid amplification tests using blood specimens or tissue swabs/biopsies, sequencing and mass-spectrometry have emerged in recent years for both pathogen and vector identification...
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29769277/rapid-screening-of-urinary-tract-infection-and-discrimination-of-gram-positive-and-gram-negative-bacteria-by-automated-flow-cytometric-analysis-using-sysmex-uf-5000
#5
Seon Young Kim, Yumi Park, Hyunjin Kim, Jimyung Kim, Sun Hoe Koo, Gye Cheol Kwon
Rapid screening of urinary tract infection is important to determine antibiotic treatment and reduce unnecessary urine culture. We evaluated the performance of the new flow cytometry-based UF-5000 automated urine analyzer (Sysmex, Kobe, Japan). A total of 1,430 urine samples from 1,226 patients were analyzed and compared with urine cultures to which a Previ Isola (bioMérieux, Marcy l'Etoile, France) system was applied. In total, 878 of 1,430 urine cultures (61.4%) produced ≥ 103 CFU/mL bacterial growth [lsqb]309 with Gram-negative (GN) bacteria, 517 with Gram-positive (GP) bacteria, and 52 mixed cultures[rsqb], with 336 samples (23...
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29769276/analytical-comparison-of-the-architect-syphilis-tp-and-liaison-treponema-assay-automated-chemiluminescent-immunoassays-and-their-performance-in-a-reverse-syphilis-screening-algorithm
#6
Alan M Sanfilippo, Kristie Freeman, John L Schmitz
Syphilis incidence has increased in the United States since 2000 (1, 2) and remains a global public health concern (3, 4).….
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29769275/-point-counterpoint-active-surveillance-for-carriers-of-toxigenic-clostridium-difficile-should-be-performed-to-guide-prevention-efforts
#7
L Clifford McDonald, Daniel J Diekema
In 2017, we published a point-counterpoint on laboratory diagnosis of C. difficile infection (CDI). At that time, Drs Ferric Fang, Christopher Polage, and Mark Wilcox discussed the strategies for diagnosing Clostridium difficile colitis in symptomatic patients. Since that manuscript new guidelines from the Infectious Diseases Society of American and the Society for Health Care Epidemiology have been published (1) and healthcare systems have begun to explore screening asymptomatic patients for C. difficile colonization...
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29769274/a-comparative-evaluation-of-four-phenotypic-methods-for-detection-of-class-a-and-b-carbapenemase-producing-enterobacteriaceae-in-china
#8
Menglan Zhou, Danchen Wang, Timothy Kudinha, Qiwen Yang, Shuying Yu, Ying-Chun Xu
Objectives: To evaluate the performance of four phenotypic methods in the detection of carbapenemase-producing Enterobacteriaceae (CPE) in China. Methods: We evaluated the performance of four carbapenemase detection methods, the modified Hodge test (MHT), Carba NP test, meropenem hydrolysis assay (MHA) with 1 and 2-hour incubation, and modified carbapenem inactivation method (mCIM) with meropenem, imipenem, and ertapenem, on 342 carbapenem-resistant Enterobacteriaceae (CRE) in China. Polymerase chain reaction was used as the gold standard...
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29743311/misidentification-of-s-aureus-by-the-cepheid-xpert%C3%A2-mrsa-sa-bc-assay-due-to-deletions-in-the-spa-gene
#9
Gar-Hing Lee, Stanley Pang, Geoffrey W Coombs
Staphylococcus aureus is a pathogenic bacterium with the capacity to cause a variety of clinical syndromes.….
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29743310/comparison-of-the-quantiferon-tb-gold-plus-and-quantiferon-tb-gold-in-tube-interferon-%C3%AE-release-assays-in-patients-at-risk-for-tuberculosis-and-in-healthcare-workers
#10
Elitza S Theel, Heather Hilgart, Margaret Breen-Lyles, Kevin McCoy, Rhiannon Flury, Laura E Breeher, John Wilson, Irene G Sia, Jennifer A Whitaker, Jeremy Clain, Timothy R Aksamit, Patricio Escalante
The QuantiFERON-TB Gold Plus (QFT-Plus; Qiagen, Germantown, MD) interferon-gamma release assay (IGRA) received FDA-clearance in 2017 and will replace the prior version of the assay, the QFT-Gold In Tube (QFT-GIT). Here, we compared performance of the QFT-Plus assay to the QFT-GIT version in a diverse patient population, including patients undergoing evaluation for or follow-up of latent tuberculosis infection (LTBI; n=39) or active TB (n=3), and in healthcare workers (HCWs; n=119) at Mayo Clinic (Rochester, MN)...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29743309/utility-of-a-stressed-snp-real-time-pcr-assay-for-the-rapid-identification-of-measles-vaccine-strain-in-patient-samples
#11
Thomas Tran, Renata Kostecki, Michael Catton, Julian Druce
Rapid differentiation of wild-type measles virus from measles vaccine strains is crucial during a measles outbreak and in a measles elimination setting. A real-time RT-PCR for the rapid detection of measles vaccine strains was developed with high specificity and greater sensitivity than when compared to traditional measles genotyping methods. The "stressed" minor grove binder TaqMan probe design approach achieves specificity to vaccine strains only, without compromising sensitivity. This assay has proven to be extremely useful in outbreak settings, without requiring sequence genotyping, for over 4 years at the Regional Measles Reference Laboratory for the Western Pacific Region...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29743308/performance-evaluation-of-a-prototype-architect-antibody-assay-for-babesia-microti
#12
Kevin Cheng, Kelly E Coller, Christopher C Marohnic, Zachary A Pfeiffer, James R Fino, Randee R Elsing, Janet Bergsma, Marilee A Marcinkus, Alak K Kar, Orlando H Gumbs, Kathy S Otis, Jeffrey Fishpaugh, Phillip W Schultz, Mark R Pope, Alfredo R Narvaez, Susan J Wong, Susan Madison-Antenucci, Thomas P Leary, George J Dawson
The tick-borne protozoan Babesia microti is responsible for more than 200 cases of transfusion-transmitted babesiosis (TTB) infection in the United States over the last 30 years. Measures to mitigate the risk of TTB include nucleic acid testing (NAT) and B. microti antibody testing. A fully automated prototype B. microti antibody test was developed on the ARCHITECT instrument. The specificity was determined to be 99.98% in volunteer blood donors (n=28,740) from areas considered as low endemic for B. microti The sensitivity of the prototype test was studied in experimentally-infected macaques; a total of 128 samples were detected compared to 125 with the indirect fluorescent antibody test (IFA), additionally, 83 (89...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29743307/evaluation-of-modified-two-tiered-testing-algorithms-for-lyme-disease-laboratory-diagnosis-using-well-characterized-serum-samples
#13
Adoracion Pegalajar-Jurado, Martin E Schriefer, Ryan J Welch, Marc R Couturier, Tiffany MacKenzie, Rebecca J Clark, Laura V Ashton, Mark J Delorey, Claudia R Molins
Standard two-tiered testing (STTT) is the recommended algorithm for laboratory diagnosis of Lyme disease (LD). Several limitations are associated with STTT that include low sensitivity in the early stages of disease, as well as technical complexity and subjectivity associated with second-tier immunoblots; therefore, modified two-tiered testing (MTTT) algorithms that utilize two sequential first-tier tests and eliminate immunoblots have been evaluated. Recently, a novel MTTT that uses a VlsE chemiluminescence immunoassay followed by a C6 enzyme immunoassay has been proposed...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29743306/reduction-in-the-percentage-of-clusters-of-candida-albicans-and-candida-parapsilosis-causing-candidemia-in-a-general-hospital-in-madrid-spain
#14
Pilar Escribano, Carlos Sánchez-Carrillo, Patricia Muñoz, Emilio Bouza, Jesús Guinea
The presence of clusters in units with a high incidence of candidemia suggests the need for prevention of candidemia. We analyzed the percentage of patients involved in clusters and its evolution over a large period of time in a tertiary hospital.We studied 432 patients admitted to Gregorio Marañón Hospital with candidemia caused by Candida albicans (n=276) or Candida parapsilosis (n=156) between January 2007 and December 2014. Incident isolates were genotyped. A cluster was defined as a group of ≥2 patients infected by an identical genotype; we considered clusters to be "tracking clusters" when the patients involved in the cluster were admitted to the same ward within a period of 24 months...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29743305/five-year-national-surveillance-of-invasive-candidiasis-species-distribution-and-azole-susceptibility-from-the-china-hospital-invasive-fungal-surveillance-net-chif-net-study
#15
Meng Xiao, Zi-Yong Sun, Mei Kang, Da-Wen Guo, Kang Liao, Sharon C-A Chen, Fanrong Kong, Xin Fan, Jing-Wei Cheng, Xin Hou, Meng-Lan Zhou, Ying Li, Shu-Ying Yu, Jing-Jing Huang, He Wang, Ying-Chun Xu
Data on the epidemiology of invasive candidiasis (IC) and antifungal susceptibility of Candida isolates in China are still limited. Here we report surveillance for IC from the China Hospital Invasive Fungal Surveillance Net (CHIF-NET) Study. Sixty-five tertiary hospitals collected 8,829 Candida isolates from August 1, 2009 to July 31, 2014. Matrix-assisted laser desorption/ionization -time of flight mass spectrometry supplemented by rDNA sequencing was used to define species, and fluconazole and voriconazole susceptibilities determined by the Clinical and Laboratory Standards Institute disk diffusion method...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29743304/molecular-characterization-of-invasive-isolates-of-neisseria-meningitidis-in-casablanca-morocco
#16
Aziza Razki, Eva Hong, Khalid Zerouali, Houria Belabbes, Khadija Aitmouss, Aude Terrade, Bahija Zaki, Ala-Eddine Deghmane, Naima Elmdaghri, Muhamed-Kheir Taha
Meningococcal epidemiology may change unpredictably and typing of isolates of Neisseria meningitidis is crucial for the surveillance of invasive meningococcal disease (IMD). Few data are available of the meningococcal epidemiology in countries of North Africa. We aimed to explore the invasive meningococcal isolates from the Casablanca region in Morocco. We used whole genome sequencing (WGS) to characterize 105 isolates from this region during the period 2011-2016. Our data showed that the majority (n=100) of the isolates belonged to serogroup B...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29743303/searching-for-a-potential-algorithm-for-clostridium-difficile-testing-at-a-tertiary-care-hospital-does-toxin-enzyme-immunoassay-testing-help
#17
Angela M Theiss, Agnes Balla, Angie Ross, Denise Francis, Christina Wojewoda
Clostridium difficile is a major contributor to morbidity and mortality in the United States. Methods for identifying the organism in stool include molecular platforms, enzyme immunoassays for toxin, and culture. Controversy persists over whether molecular tests are too sensitive at identifying C. difficile and have raised questions about how additional laboratory information could inform clinical management and reduce over treatment. The aim of this study was to assess whether clinical factors were related to toxin status of patients and whether information about toxin status could potentially inform clinical management of patients...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29743302/the-continued-value-of-disk-diffusion-for-assessing-antimicrobial-susceptibility-in-clinical-laboratories-report-from-the-clinical-and-laboratory-standards-institute-methods-development-and-standardization-working-group
#18
Romney M Humphries, Susan Kircher, Andrea Ferrell, Kevin M Krause, Rianna Malherbe, Andre Hsiung, C A Burnham
Expedited pathways to antimicrobial agent approval by the United States Food and Drug Administration (FDA) have led to increased delays between drug approval and the availability of FDA-cleared antimicrobial susceptibility testing (AST) devices. Antimicrobial disks for use with disk diffusion testing are among the first AST devices available to clinical laboratories. However, many laboratories are reluctant to implement a disk diffusion method for a variety of reasons, including dwindling proficiency with this method, interruptions to laboratory workflow, uncertainty surrounding the quality and reliability of a disk diffusion test, and perceived need to report an MIC to clinicians...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29720434/evaluation-of-a-turbidimetric-%C3%AE-d-glucan-test-for-the-detection-of-pneumocystis-jirovecii-pneumonia
#19
Karl Dichtl, Ulrich Seybold, Johannes Wagener
Currently, diagnosis of Pneumocystis jirovecii pneumonia (PJP) relies on analysis of lower respiratory specimens, either by microscopy or qPCR. Thus, bronchoscopy is required, which is associated with increased risk of respiratory failure. We assessed the value of non-invasive serologic β-D-glucan (BDG) testing for laboratory diagnosis of PJP using a newly available turbidimetric assay. We identified 73 cases of PJP with positive qPCR results from lower respiratory specimens for Pneumocystis and serology samples dating from 1 week before to 4 weeks after qPCR...
May 2, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29720433/evaluation-of-the-use-of-rectal-swabs-for-the-laboratory-diagnosis-of-clostridium-difficile-infection
#20
N Jazmati, E Kirpal, E Piepenbrock, Y Stelzer, M J G T Vehreschild, H Seifert
Background: For the diagnosis of Clostridium difficile infection (CDI), microbiological testing is almost always accomplished through analysis of stool specimens. We evaluated the performance of rectal swabs with liquid transport medium (FS) and nylon flocked dry swabs for the detection of C. difficile Additionally, the impact on the diagnostic yield of storing swabs at -80°C for up to three months was evaluated. Methods: Sixty clinical stool samples positive for C. difficile by PCR were used for simulating rectal swabbing...
May 2, 2018: Journal of Clinical Microbiology
journal
journal
26018
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"