Read by QxMD icon Read

Journal of Clinical Microbiology

Kristine M Wylie, Todd N Wylie, Richard Buller, Brandi Herter, Maria T Cannella, Gregory A Storch
Metagenomic shotgun sequencing (MSS) is a revolutionary approach to viral diagnostic testing that allows simultaneous detection of a broad range of viruses, detailed taxonomic assignment, and detection of mutations associated with antiviral drug resistance. To enhance sensitivity for virus detection, we previously developed ViroCap, a targeted sequence capture panel designed to enrich nucleic acid from a comprehensive set of eukaryotic viruses prior to sequencing. To demonstrate the utility of MSS with targeted sequence capture for detecting clinically important viruses and characterizing clinically important viral features, we used ViroCap to analyze clinical samples from a diagnostic virology laboratory containing a broad range of medically relevant viruses...
September 19, 2018: Journal of Clinical Microbiology
S A R Siegel, M Cavanaugh, J H Ku, L M Kawamura, K L Winthrop
Interferon gamma releases assays (IGRAs) are important tools in identifying prior tuberculosis exposure. The new generation QuantiFERON®-TB Gold Plus (QFT-Plus), recently approved for use in the U.S., differs from the current generation QFT Gold-In-Tube® (QFT-GIT), with the addition of a second antigen tube that also contains novel CD8+ T-cell stimulating peptides. The QFT-Plus has increased sensitivity in the immunocompromised populations, and we sought to assess specificity of QFT-Plus compared to QFT-GIT in low-risk individuals...
September 19, 2018: Journal of Clinical Microbiology
Vanessa da Silva Brito, Fred Luciano Neves Santos, Noilson Lazaro Sousa Gonçalves, Thessika Hialla Araujo, Davi Silva Vale Nascimento, Felicidade Mota Pereira, Ney Cristian Amaral Boa-Sorte, Maria Fernanda Rios Grassi, Adele Caterino-de-Araujo, Bernardo Galvão-Castro
Serological screening for HTLV-1 is usually performed using enzyme-linked immunosorbent assay, particle agglutination or chemiluminescence assay kits. Due to antigen matrix improvement entailing the use of new HTLV-antigens and changes in the format of HTLV screening tests, as well as newly introduced CLIAs, a systematic evaluation of the accuracy of currently available commercial tests is warranted. We aimed to assess the performance of commercially available screening tests for HTLV diagnosis. A diagnostic accuracy study was conducted on a panel of 397 plasma samples: 200 HTLV-negative, 170 HTLV-positive and 27 indeterminate under Western blotting analysis...
September 19, 2018: Journal of Clinical Microbiology
D Joseph Sexton, Milena Kordalewska, Meghan L Bentz, Rory M Welsh, David S Perlin, Anastasia P Litvintseva
The recent emergence of the multidrug-resistant and pathogenic yeast Candida auris continues to cause public health concern worldwide. C. auris is alarming because it causes healthcare-associated outbreaks and can establish invasive infections with high mortality rates. Transmission between patients is facilitated by the ability of C. auris to persistently colonize multiple body sites, including the skin, and survive for weeks on surfaces in healthcare settings. Rapid identification of colonized patients is needed to implement timely infection control measures...
September 19, 2018: Journal of Clinical Microbiology
Toshio Ohshima, Takako Osaki, Yukari Yamamoto, Satomi Asai, Hayato Miyachi, Shigeru Kamiya
In recent years, the diagnostic method of choice for Clostridium difficile infection (CDI) is a rapid enzyme immunoassay in which glutamate dehydrogenase (GDH) antigen and C. difficile toxin can be detected ( C. DIFF QUIK CHEK COMPLETE®: Alere Inc.) (QUIK CHEK). However, the clinical significance remains unclear in cases which demonstrate a positive result for GDH antigen and are negative for toxin. In this study, we used the QUIK CHEK test kit on fecal samples with an additional toxin detection step using toxigenic culture assay for the above cases...
September 19, 2018: Journal of Clinical Microbiology
Eric A Gehrie
Although transfusion-transmitted bacterial infection is relatively rare, mitigation of bacterial contamination of platelet units is arguably the top current transfusion-related safety concern. Several different technologies have been employed to detect or neutralize bacteria in platelet concentrates. However, studies of the efficacy of these systems have been hampered by problematic definitions of what represents a "true" versus a "false" positive culture result. In the current issue of the Journal of Clinical Microbiology , M...
September 19, 2018: Journal of Clinical Microbiology
Steven M Lada, Karissa Huang, D Jake VanBelzen, Luis J Montaner, Una O'Doherty, Douglas D Richman
We utilized pulsed-field gel electrophoresis (PFGE) to purify high molecular weight DNA from HIV infected cells. This purification, in combination with our previously described droplet digital PCR (ddPCR) assay, was used to develop a method to quantify proviral integrated HIV DNA free of lower molecular weight species of HIV DNA. Episomal 2-LTR circles were completely cleared from HIV DNA samples. Technical replicates of the complete assay starting with the same specimen resulted in no statistical differences in quantification of integrated HIV Gag sequence in cellular DNA from cells from HIV-infected subjects after prolonged treatment with antiretroviral therapy (ART)...
September 19, 2018: Journal of Clinical Microbiology
Chiou Horng Ong, Lasantha Ratnayake, Michelle L T Ang, Raymond Tzer Pin Lin, Douglas Su Gin Chan
Rapid and accurate detection of carbapenemase producing Enterobacteriaceae (CPE) is necessary for patient management and infection control measures. We compared the performance of the BD Phoenix CPO Detect with a home-made CarbaNP and modified carbapenem inactivation method (mCIM) by challenging all 3 assays with 190 isolates of Enterobacteriaceae with meropenem MICs of >0.125mg/l. 160 isolates produced KPC, IMI-1, NDM, IMP and OXA-type carbapenemases while 30 were negative for carbapenemase production. Sensitivity and Specificity were 90...
September 12, 2018: Journal of Clinical Microbiology
Maria Dudareva, Lucinda Barrett, Mel Figtree, Matthew Scarborough, Masanori Watanabe, Robert Newnham, Rachael Wallis, Sarah Oakley, Ben Kendrick, David Stubbs, Martin A McNally, Philip Bejon, Bridget A Atkins, Adrian Taylor, Andrew J Brent
BACKGROUND: Current guidelines recommend collection of multiple tissue samples for diagnosis of prosthetic joint infections (PJI). Sonication of explanted devices has been proposed as a potentially simpler alternative, however reported microbiological yield varies. METHODS: We evaluated sonication for diagnosis of PJI and other orthopaedic device-related infections (DRI) at the Oxford Bone Infection Unit between October 2012 and August 2016. We compared the performance of paired tissue and sonication cultures against a 'gold-standard' of published clinical and composite clinical and microbiological definitions of infection...
September 12, 2018: Journal of Clinical Microbiology
Anja Oštrbenk, Lan Xu, Marc Arbyn, Mario Poljak
In 2012 the VALidation of human papillomavirus (HPV) GENotyping Tests (VALGENT) was initiated to provide a formalized and uniform study framework for comparison and validation of HPV assays with genotyping capability. In VALGENT-3, the clinical and analytical performance of Anyplex II HPV HR Detection (Anyplex) was compared to Hybrid Capture 2 HPV DNA Test (hc2) and the cobas 4800 HPV Test (cobas). The panel comprises 1,300 stored samples that were obtained from women 25 to 64 years old that participated in the Slovenian cancer screening program enriched with 300 samples from women with abnormal cervical cytology...
September 12, 2018: Journal of Clinical Microbiology
David H Wyllie, Esther Robinson, Tim Peto, Derrick W Crook, Adebisi Ajileye, Priti Rathod, Rosemarie Allen, Lisa Jarrett, E Grace Smith, A Sarah Walker
Introduction: Detecting laboratory cross-contamination and mixed tuberculosis infection are important goals of clinical Mycobacteriology laboratories. Objectives: To develop a method detecting mixtures of different M. tuberculosis lineages in laboratories performing Mycobacterial next generation sequencing (NGS). Setting: Public Health England National Mycobacteriology Laboratory Birmingham, which performs Illumina sequencing on DNA extracted from positive Mycobacterial Growth Indicator tubes. Methods: We analysed 4,156 samples yielding M...
September 12, 2018: Journal of Clinical Microbiology
Susanne Karlsmose Pedersen, Jaap A Wagenaar, Håkan Vigre, Louise Roer, Matthew Mikoleit, Awa Aidara-Kane, Amy L Cawthorne, Frank M Aarestrup, Rene S Hendriksen
Campylobacter spp. are food- and water borne pathogens. While rather accurate estimates for these pathogens are available in industrialized countries, a lack of diagnostic capacity in developing countries limits accurate assessments of prevalence in many regions. Proficiency in the identification and susceptibility testing of these organisms is critical for surveillance and control efforts. The aim of the study was to assess performance for identification and susceptibility testing of thermo-tolerant Campylobacter among laboratories participating in the World Health Organization (WHO) Global Foodborne Infections Network (GFN) External Quality Assurance System (EQAS) over a nine year period...
September 12, 2018: Journal of Clinical Microbiology
Betty A Forbes
A thread woven through input provided from colleagues is Dr. Ellen Jo Baron's limitless energy.….
September 5, 2018: Journal of Clinical Microbiology
C Kolenda, R Benoit, A Carricajo, R Bonnet, O Dauwalder, F Laurent
The worldwide emergence of highly resistant bacteria such as carbapenemase-producing Enterobacteriaceae is a major public health concern (1).….
September 5, 2018: Journal of Clinical Microbiology
Petr Kodym, Zuzana Kurzová, Dagmar Berenová, Dušan Pícha, Dita Smíšková, Lenka Moravcová, Marek Malý
The study compares diagnostic parameters of different commercial serological kits based on three different antigen types and correlates test results with the status of the patient's Borrelia infection.In total, 8 IgM and 8 IgG kits were tested: ELISA (EUROIMMUN) based on whole-cell antigen, 3 species-specific EIAs (TestLine), LIAISON chemiluminescence (DiaSorin), ELISA-VIDITEST (Vidia), EIA and BLOT-LINE (TestLine) using recombinant antigens. All tests were performed on a panel of 90 samples from patients with clinically characterized borreliosis (53 x neuroborreliosis, 32 x erythema migrans, 5 x arthritis) plus 70 controls from blood donors and syphilis patients...
September 5, 2018: Journal of Clinical Microbiology
Dithi Banerjee, Neena Kanwar, Ferdaus Hassan, Cynthia Essmyer, Rangaraj Selvarangan
Rapid and accurate detection of influenza A/B and respiratory syncytial virus (RSV) improves patient care. Sample-to-answer (STA) platforms based on nucleic acid amplification and detection of these viruses are simple, automated, and accurate. We compared six such platforms for the detection of FluA, FluB, and RSV: Cepheid GeneXpert® Xpress Flu/RSV (Xpert), Hologic Panther Fusion® Flu A/B/RSV (Fusion), Cobas® Influenza A/B & RSV (Liat), Luminex Aries® Flu A/B & RSV (Aries), Biofire FilmArray® Respiratory Panel (RP) and Diasorin Simplexa™ Flu A/B & RSV (Simplexa)...
September 5, 2018: Journal of Clinical Microbiology
Inge Roof, Rana Jajou, Miranda Kamst, Arnout Mulder, Albert de Neeling, Rianne van Hunen, Wim van der Hoek, Dick van Soolingen
Introduction: The variable number of tandem repeats (VNTR) typing method is used to study tuberculosis (TB) transmission. Clustering of Mycobacterium tuberculosis isolates with identical VNTR patterns is assumed to reflect recent transmission. Hence, clusters are thought to be homogeneous regarding antibiotic resistance. In practice, however, also heterogeneous clusters are identified. This study investigates the prevalence and characteristics of heterogeneous VNTR clusters and assesses whether isolates in these clusters remain clustered when subjected to whole genome sequencing (WGS)...
August 29, 2018: Journal of Clinical Microbiology
Johanna Sandlund, Amelita Bartolome, Anna Almazan, Stanley Tam, Sheryl Biscocho, Salina Abusali, Jeff Bishop, Niamh Nolan, Joel Estis, John Todd, Stephen Young, Fiona Senchyna, Niaz Banaei
Background: Current tests for the detection of Clostridoides (formerly Clostridium ) difficile free toxins in feces lack sensitivity, while nucleic acid amplification tests lack clinical specificity. We have evaluated the Singulex Clarity® C. diff toxins A/B assay (currently in development), an automated and rapid ultrasensitive immunoassay powered by Single Molecule Counting technology, for detection of C. difficile toxins A (TcdA) and B (TcdB) in stool. Method: Analytical sensitivity, analytical specificity, repeatability, and stability for the assay were determined...
August 29, 2018: Journal of Clinical Microbiology
Pranita D Tamma, Patricia J Simner
The rapid spread of multidrug-resistant Gram-negative organisms constitutes one of the greatest challenges to global health. While Gram-negative organisms have developed several mechanisms to avert the bactericidal effects of commonly prescribed antibiotic agents, the increasing prevalence of carbapenemase-producing organisms (CPO) is particularly concerning given the rapid spread of mobile genetic elements containing carbapenemase genes, the limited treatment options for infections caused by these organisms, and the high mortality rates associated with CPO infections...
August 29, 2018: Journal of Clinical Microbiology
Kelly F Oakeson, Jennifer Marie Wagner, Andreas Rohrwasser, Robyn Atkinson-Dunn
Whole genome sequencing (WGS) via next generation sequencing (NGS) technologies is a powerful tool for determining the relatedness of bacterial isolates in foodborne illness detection and outbreak investigations. WGS has been applied to national outbreaks, for example Listeria monocytogenes , however, WGS has rarely been used in smaller local outbreaks.The current study demonstrates the superior resolution of genetic and evolutionary relatedness generated by WGS data analysis when compared to pulsed-field gel electrophoresis (PFGE)...
August 29, 2018: Journal of Clinical Microbiology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"