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Memórias do Instituto Oswaldo Cruz

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https://www.readbyqxmd.com/read/28403328/zika-puzzle-in-brazil-peculiar-conditions-of-viral-introduction-and-dissemination-a-review
#1
Cristina Possas, Patricia Brasil, Mauro Ca Marzochi, Amilcar Tanuri, Reinaldo M Martins, Ernesto Ta Marques, Myrna C Bonaldo, Antonio Gp Ferreira, Ricardo Lourenço-de-Oliveira, Rita Maria R Nogueira, Patricia C Sequeira, Keyla Bf Marzochi, Akira Homma
This article discusses the peculiar conditions that favoured the unexpected introduction of Zika virus into the poorest northeastern region of Brazil in 2015, its speed of transmission to other Brazilian states, other Latin American countries and other regions, and the severity of related neurological disorders in newborns and adults. Contrasting with evidence that Zika had so far caused only mild cases in humans in the last six decades, the epidemiological scenario of this outbreak in Brazil indicates dramatic health effects: in 2015, an increase of 20-fold in notified cases of microcephaly and/or central nervous system (CNS) alterations suggestive of Zika congenital infection, followed by an exponential increase in 2016, with 2366 cumulative cases confirmed in the country by the end of December 2016...
April 6, 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28403327/internal-control-for-real-time-polymerase-chain-reaction-based-on-ms2-bacteriophage-for-rna-viruses-diagnostics
#2
Miriam Ribas Zambenedetti, Daniela Parada Pavoni, Andreia Cristine Dallabona, Alejandro Correa Dominguez, Celina de Oliveira Poersch, Stenio Perdigão Fragoso, Marco Aurélio Krieger
BACKGROUND: Real-time reverse transcription polymerase chain reaction (RT-PCR) is routinely used to detect viral infections. In Brazil, it is mandatory the use of nucleic acid tests to detect hepatitis C virus (HCV), hepatitis B virus and human immunodeficiency virus in blood banks because of the immunological window. The use of an internal control (IC) is necessary to differentiate the true negative results from those consequent from a failure in some step of the nucleic acid test. OBJECTIVES: The aim of this study was the construction of virus-modified particles, based on MS2 bacteriophage, to be used as IC for the diagnosis of RNA viruses...
April 6, 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28403326/characterisation-of-the-vascular-pathology-in-sigmodon-hispidus-rodentia-cricetidae-following-experimental-infection-with-angiostrongylus-costaricensis-nematoda-metastrongylidae
#3
Danielle Ingrid Bezerra de Vasconcelos, Ester Maria Mota, Marcelo Pelajo-Machado
BACKGROUND: Angiostrongylus costaricensis is a nematode that causes human abdominal angiostrongyliasis, a disease found mainly in Latin American countries and particularly in Brazil and Costa Rica. Its life cycle involves exploitation of both invertebrate and vertebrate hosts. Its natural reservoir is a vertebrate host, the cotton rat Sigmodon hispidus. The adult worms live in the ileo-colic branches of the upper mesenteric artery of S. hispidus, causing periarteritis. However, there is a lack of data on the development of vasculitis in the course of infection...
April 6, 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591316/molecular-detection-of-mixed-infections-with-multiple-dengue-virus-serotypes-in-suspected-dengue-samples-in-tamaulipas-mexico
#4
LETTER
Rocío Requena-Castro, Miguel Ángel Reyes-López, Rosa Eminé Rodríguez-Reyna, Prisco Palma-Nicolás, Virgilio Bocanegra-García
This study aimed to detect dengue virus (DENV) serotypes in serum samples obtained in Matamoros Tamaulipas, Mexico, and to determine the concordance of conventional nested reverse transcriptase polymerase chain reaction (RT-PCR) and a serological test [enzyme-linked immunosorbent assay (ELISA NS1)]. Here, we detected mixed infections consisting of four serotypes of DENV. The most prevalent serotype was DENV-1, followed by DENV-4. This is the first report of DENV-4 in our region. Mixed infections were also detected in 21...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591315/trichophoromyia-auraensis-is-a-putative-vectorcomments-about-the-finding-of-trichophoromyia-auraensis-mangabeira-1942-sandfly-specimens-infected-by-leishmania-viannia-parasites-in-acre-statea-new-sandfly-in-the-subgenus-nyssomyia-diptera-psychodidae-from-the
#5
LETTER
Trichophoromyia auraensis is a putative vectorComments about the finding of Trichophoromyia auraensis (Mangabeira 1942) sandfly specimens infected by Leishmania (Viannia) parasites in Acre stateA new sandfly in the subgenus Nyssomyia (Diptera, Psychodidae) from the Amazon Basin of BrazilThe known geographical distribution of sandflies in the state of Acre, Brazil (Diptera: Psychodidae)Studies on the sandfly fauna (Diptera: Psychodidae: Phlebotominae) from transmission areas of American cutaneous leishmaniasis in state of Acre, BrazilDevelopment and validation of PCR-based assays for diagnosis of American cutaneous leishmaniasis and identification of the parasite speciesFirst description of Leishmania (Viannia) infection in Evandromyia saulensis, Pressatia sp. and Trichophoromyia auraensis (Psychodidae: Phlebotominae) in a transmission area of cutaneous leishmaniasis in Acre state, Amazon Basin, BrazilSand fly fauna (Diptera: Psychodidae: Phlebotominae) in an area of leishmaniasis transmission in the municipality of Rio Branco, state of Acre, BrazilIdentification of naturally infected Lutzomyia intermedia and Lutzomyia migonei with Leishmania (Viannia) braziliensis in Rio de Janeiro (Brazil) revealed by a PCR multiplex non-isotopic hybridization assayNotas sobre os flebotomíneos do estado do Acre, com a descrição de duas espécies novas (Diptera, Psychodidae)Description of Trichophoromyia ruifreitasi, a new phlebotomine species (Diptera, Psychodidae) from Acre state, Brazilian AmazonEpidemiologia da Leishmaniose Tegumentar e descrição das populações de flebotomíneos no município de Acrelândia, Acre, BrasilPhlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and BoliviaDescription of a new phlebotomine species (Diptera: Psychodidae, Phlebotominae) and new records of sand flies from the state of Acre, Northern BrazilNatural Leishmania infection of Lutzomyia (Trichophoromyia) auraensis in Madre de Dios, Peru, detected by a fluorescence resonance energy transfer-based real-time polymerase chain reactionGuide to the identification and geographic distribution of Lutzomyia sandflies in Mexico, the West Indies, Central and South America (Diptera: Psychodidae).
Carolina Bioni Garcia Teles, Felipe Arley Costa Pessoa, Jansen Fernandes Medeiros, Luís Marcelo Aranha Camargo
The sandfly Trichophoromyia auraensis has recently evolved as a proven vector of Leishmania (Viannia) endemic to state of Acre in the north of Brazil. This note is intended to propose a correction in the report of the first occurrence of natural infection of Leishmania (Viannia) in this species. We and the other scientific groups reinforced that Tr. auraensis is a possible vector involved in the transmission of American cutaneous leishmaniasis in Acre, Brazil.
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591314/complete-plasmid-sequence-carrying-type-iv-like-and-type-vii-secretion-systems-from-an-atypical-mycobacteria-strain
#6
Sergio Mascarenhas Morgado, Michel Abanto Marín, Fernanda S Freitas, Erica Lourenço Fonseca, Ana Carolina Paulo Vicente
The genus Mycobacterium is highly diverse and ubiquitous in nature, comprehending fast- and slow-growing species with distinct impact in public health. The plasmid-mediated horizontal gene transfer represents one of the major events in bacteria evolution. Here, we report the complete sequence of a 160,489 bp circular plasmid (pCBMA213_2) from an atypical and fast-growing environmental mycobacteria. This is a unique plasmid, in comparison with the characterised mycobacteria plasmids, harboring a type IV-like and ESX-P2 type VII secretion systems...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591313/multiplexed-reverse-transcription-real-time-polymerase-chain-reaction-for-simultaneous-detection-of-mayaro-oropouche-and-oropouche-like-viruses
#7
Felipe Gomes Naveca, Valdinete Alves do Nascimento, Victor Costa de Souza, Bruno Tardelli Diniz Nunes, Daniela Sueli Guerreiro Rodrigues, Pedro Fernando da Costa Vasconcelos
We describe a sensitive method for simultaneous detection of Oropouche and Oropouche-like viruses carrying the Oropouche S segment, as well as the Mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (RT-qPCR). A chimeric plasmid containing both Mayaro and Oropouche targets was designed and evaluated for the in vitro production of transcribed RNA, which could be easily used as a non-infectious external control. To track false-negative results due to PCR inhibition or equipment malfunction, the MS2 bacteriophage was also included in the multiplex assay as an internal positive control...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591312/identification-of-a-type-i-nitroreductase-gene-in-non-virulent-trypanosoma-rangeli
#8
Marjorie Montenegro, Claudia Cuervo, Constanza Cardenas, Silvia Duarte, Jenny R Díaz, M Carmen Thomas, Manuel C Lopez, Concepcion J Puerta
Trypanosomatid type I nitroreductases (NTRs), i.e., mitochondrial enzymes that metabolise nitroaromatic pro-drugs, are essential for parasite growth, infection, and survival. Here, a type I NTR of non-virulent protozoan Trypanosoma rangeli is described and compared to those of other trypanosomatids. The NTR gene was isolated from KP1(+) and KP1(-) strains, and its corresponding transcript and 5' untranslated region (5'UTR) were determined. Bioinformatics analyses and nitro-drug activation assays were also performed...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591311/participation-of-the-arcracme-protein-in-self-activation-of-the-arc-operon-located-in-the-arginine-catabolism-mobile-element-in-pandemic-clone-usa300
#9
Zayda Lorena Corredor Rozo, Ricaurte Alejandro Márquez-Ortiz, Betsy Esperanza Castro, Natasha Vanegas Gómez, Javier Escobar-Pérez
Staphylococcus aureus pandemic clone USA300 has, in addition to its constitutive arginine catabolism (arc) gene cluster, an arginine catabolism mobile element (ACME) carrying another such cluster, which gives this clone advantages in colonisation and infection. Gene arcR, which encodes an oxygen-sensitive transcriptional regulator, is inside ACME and downstream of the constitutive arc gene cluster, and this situation may have an impact on its activation. Different relative expression behaviours are proven here for arcRACME and the arcACME operon compared to the constitutive ones...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591310/human-papillomavirus-type-distribution-and-hpv16-intratype-diversity-in-southern-brazil-in-women-with-and-without-cervical-lesions
#10
Gisele R de Oliveira, Valdimara C Vieira, Emiliana C Ávila, Fabiana Finger-Jardim, Thaís Dm Caldeira, Fabiane Aa Gatti, Carla V Gonçalves, Sandro G Oliveira, Vanusa P da Hora, Marcelo A Soares, Ana Mb de Martinez
BACKGROUND: Increasing evidence suggests that human papillomavirus (HPV) intratype variants (specific lineages and sublineages) are associated with pathogenesis and progression from HPV infection to persistence and the development of cervical cancer. OBJECTIVES: This study aimed to verify the prevalence of HPV infection and distribution of HPV types and HPV16 variants in southern Brazil in women with normal cytology or intraepithelial lesions. METHODS: HPV typing was determined by L1 gene sequencing...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591309/detection-and-molecular-characterisation-of-a-diagnosis-escape-variant-associated-with-occult-hepatitis-b-virus-in-brazil
#11
Ricardo Wagner de Almeida, Francisco Campello do Amaral Mello, Isabelle Vasconcelos Menegoy, Márcia Paschoal do Espírito Santo, Cléber Ferreira Ginuíno, Paulo Sérgio Fonseca de Sousa, Livia Melo Villar, Elisabeth Lampe, Lia Laura Lewis-Ximenez
BACKGROUND: Many studies have identified mutations in the hepatitis B surface antigen (HBsAg) as important factors limiting the ability of commercial serological assays to detect this viral antigen. However, an association between mutations in the HBsAg gene and the occurrence of occult HBV infection (OBI) in patients has not been established. OBJECTIVES: To detect hepatitis B virus (HBV) DNA in patients with anti-HBc as a unique serological marker, a previously published, cost-effective TaqMan-based real-time polymerase chain reaction (PCR) test with minor groove binding probes was adapted for use in this study...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591308/prevalence-of-tuberculosis-respiratory-symptoms-and-associated-factors-in-the-indigenous-populations-of-paraguay-2012
#12
Sarita Aguirre, Celia Martínez Cuellar, María Belén Herrero, Gustavo Chamorro Cortesi, Nilda Gimenez de Romero, Mirian Alvarez, Jose Ueleres Braga
BACKGROUND: The prevalence of respiratory symptoms and confirmed tuberculosis (TB) among indigenous groups in Paraguay is unknown. METHODS: This study assessed the prevalence of respiratory symptoms, confirmed pulmonary TB, and associated socio-economic factors among indigenous Paraguayan populations. Indigenous persons residing in selected communities were included in the study. A total of 24,352 participants were interviewed at home between October and December 2012...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591307/schistosomiasis-and-hepatopulmonary-syndrome-the-role-of-concomitant-liver-cirrhosis
#13
Liana Gonçalves-Macedo, Edmundo Pessoa Lopes, Ana Lucia Coutinho Domingues, Brivaldo Markman, Vitor Gomes Mota, Carlos Feitosa Luna
BACKGROUND: Hepatopulmonary syndrome (HPS) is defined as an oxygenation defect induced by intrapulmonary vasodilation in patients with liver disease or portal hypertension. It is investigated in patients with liver cirrhosis and less frequently in those with portal hypertension without liver cirrhosis, as may occur in hepatosplenic schistosomiasis (HSS). OBJECTIVES: To investigate the prevalence of HPS in patients with HSS, and to determine whether the occurrence of HPS is influenced by concomitant cirrhosis...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591408/decrease-in-dengue-virus-2-infection-and-reduction-of-cytokine-chemokine-production-by-uncaria-guianensis-in-human-hepatocyte-cell-line-huh-7
#14
Cíntia da Silva Mello, Ligia Maria Marino Valente, Thiago Wolff, Raimundo Sousa Lima-Junior, Luciana Gomes Fialho, Cintia Ferreira Marinho, Elzinandes Leal Azeredo, Luzia Maria Oliveira-Pinto, Rita de Cássia Alves Pereira, Antonio Carlos Siani, Claire Fernandes Kubelka
BACKGROUND: Dengue fever may present hemorrhages and cavitary effusions as result of exacerbated immune responses. We investigated hydro-alcoholic extracts from leaves (UGL) and bark (UGB) of the medicinal species Uncaria guinanensis with respect to antiviral effects in Dengue virus (DENV) infection and in immunological parameters associated with in vivo physiopathological features. METHODS: Chemical profiles from UGB or UGL were compared in thin layer chromatography and 1H nuclear magnetic resonance using flavonoid compounds and a pentacyclic oxindole alkaloid-enriched fraction as references...
June 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591407/insights-from-tissue-specific-transcriptome-sequencing-analysis-of-triatoma-infestans
#15
Leilane O Gonçalves, de Oliveira Luciana M, C D'Ávila Pessoa Grasielle, Aline Cl Rosa, Marinely G Bustamante, Carlota J Belisário, Daniela M Resende, Lileia G Diotaiuti, Jeronimo C Ruiz
Triatoma infestans is an insect of subfamily Triatominae (Hemiptera: Reduviidae) and an important vector of Trypanosoma cruzi, the etiologic agent of human Chagas disease. In this work we reported a transcriptome assembly and annotation of T. infestans heads obtained by Next Generation Sequencing (NGS) technologies.
June 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591406/the-use-of-circulating-cathodic-antigen-rapid-test-and-serology-for-diagnosis-of-active-schistosoma-mansoni-infection-in-migrants-in-italy-a-non-endemic-country-a-cross-sectional-study
#16
Laura Infurnari, Laura Galli, Alba Bigoloni, Alessia Carbone, Stefania Chiappetta, Angelo Sala, Norberto Ceserani, Adriano Lazzarin, Antonella Castagna, Giovanni Gaiera/
Diagnosis of schistosomiasis in migrants coming from endemic areas can be difficult, especially in asymptomatic subjects. Light-intensity disease, in fact, may be missed due to the low sensitivity of the stool microscopy and serologic testing cannot distinguish between a resolved infection and an active infection in patients who have been infected and treated in the past, because specific antibodies can persist despite cure. We describe a cross-sectional study conducted on 82 migrants tested for Schistosoma mansoni on single blood (anti-schistosome antibodies, total IgE) and urine [point-of-care (POC) circulating-cathodic-antigen (CCA) test] samples...
June 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591405/genome-analysis-of-yellow-fever-virus-of-the-ongoing-outbreak-in-brazil-reveals-polymorphisms
#17
Myrna C Bonaldo, Mariela Martínez Gómez, Alexandre Ac Dos Santos, Filipe Vieira Santos de Abreu, Anielly Ferreira-de-Brito, Rafaella Moraes de Miranda, Marcia Gonçalves de Castro, Ricardo Lourenço-de-Oliveira
The current yellow fever outbreak in Brazil is the most severe one in the country in recent times. It has rapidly spread to areas where YF virus (YFV) activity has not been observed for more than 70 years and vaccine coverage is almost null. Here, we sequenced the whole YFV genome of two naturally infected howler-monkeys (Alouatta clamitans) obtained from the Municipality of Domingos Martins, state of Espírito Santo, Brazil. These two ongoing-outbreak genome sequences are identical. They clustered in the 1E sub-clade (South America genotype I) along with the Brazilian and Venezuelan strains recently characterised from infections in humans and non-human primates that have been described in the last 20 years...
June 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591404/sexual-transmission-of-american-trypanosomiasis-in-humans-a-new-potential-pandemic-route-for-chagas-parasites
#18
Perla F Araujo, Adriana B Almeida, Carlos F Pimentel, Adriano R Silva, Alessandro Sousa, Sebastião A Valente, Vera C Valente, Manuela M Britto, Ana C Rosa, Rozeneide M Alves, Luciana Hagström, Antonio Rl Teixeira
BACKGROUND: The Trypanosoma cruzi infection endemic in Latin America has now spread to several countries across four continents; this endemic involves triatomine vector-free protists. We hypothesised that the sexual transmission of T. cruzi contributes to the ongoing spread of Chagas disease. OBJECTIVES: A short-term longitudinal study was conducted to evaluate this hypothesis. METHODS: The study population comprised 109 subjects from four families, among whom 21 had been diagnosed with acute Chagas disease by direct parasitological analysis...
June 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591403/preliminary-multiplex-microarray-igg-immunoassay-for-the-diagnosis-of-toxoplasmosis-and-rubella
#19
Priscila T Baschirotto, Marco A Krieger, Leonardo Foti
BACKGROUND: During pregnancy, toxoplasmosis and rubella can cause serious damage to the mother and the foetus through vertical transmission. Early diagnosis enables implementation of health measures aimed at preventing vertical transmission and minimising damage caused by these diseases. OBJECTIVE: Here, we report the development of a multiplex assay for simultaneous detection of IgG antibodies produced during toxoplasmosis and rubella infection. METHODS: This assay is based on xMap technology...
June 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591402/towards-leprosy-elimination-by-2020-forecasts-of-epidemiological-indicators-of-leprosy-in-corrientes-a-province-of-northeastern-argentina-that-is-a-pioneer-in-leprosy-elimination
#20
Elisa Petri de Odriozola, Ana María Quintana, Victor González, Roque Antonio Pasetto, María Eugenia Utgés, Octavio Augusto Bruzzone, María Rosa Arnaiz
BACKGROUND: Corrientes, a province of northeastern Argentina with endemic leprosy, has improved its epidemiological indicators, however, a study of the dynamics over time is lacking. OBJECTIVES: We analysed data of 1308 leprosy patients between 1991 to 2014, and the forecast for 2020. METHODS: Descriptive statistics and stepwise Bayesian model selection were performed. Forecasts were made using the median of 100,000 projections using the parameters calculated via Monte Carlo methods...
June 2017: Memórias do Instituto Oswaldo Cruz
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