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Memórias do Instituto Oswaldo Cruz

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https://www.readbyqxmd.com/read/28767985/surveillance-of-deaths-caused-by-arboviruses-in-brazil-from-dengue-to-chikungunya
#1
LETTER
Luciano Pamplona de Góes Cavalcanti, André Ricardo Ribas Freitas, Patrícia Brasil, Rivaldo Venâncio da Cunha
Did death occur DUE TO dengue, or in a patient WITH dengue virus infection? It seems a matter of semantics, but in fact, it underscores how challenging it is to distinguish whether the disease contributed to death, or was itself the underlying cause of death. Can a death be attributed to chikungunya virus, when some deaths occur after the acute phase? Did the virus decompensate the underlying diseases, leading to death? Did prolonged hospitalisation lead to infection, resulting in the patient's progression to death? Were there iatrogenic complications during patient care? The dengue question, for which there has not yet been a definitive response, resurfaces prominently under the chikungunya surveillance scenario...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28767984/complete-coding-sequence-of-dengue-virus-serotype-4-isolated-from-field-caught-mosquitoes-in-thailand
#2
Thikhumporn Sittivicharpinyo, Passorn Wonnapinij, Wunrada Surat
This report is the first to characterise the complete coding sequence of a dengue virus serotype 4 (DENV-4) genotype I that was isolated from field-caught mosquitoes from an endemic area in Thailand in June 2013. The sequence was assembled from high-throughput sequencing reads generated by Illumina HiSeq. Three out of four observed intra-sample variants caused an amino acid variation in C, NS2B, and NS5 genes. The C4279T variant located in the NS2B gene can indirectly affect the proteolytic activity of the NS3 protein...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28767983/evidence-for-regulated-expression-of-telomeric-repeat-containing-rnas-terra-in-parasitic-trypanosomatids
#3
Jeziel D Damasceno, Gabriel LA Silva, Christian Tschudi, Luiz Ro Tosi
The Telomeric Repeat-containing RNAs (TERRA) participate in the homeostasis of telomeres in higher eukaryotes. Here, we investigated the expression of TERRA in Leishmania spp. and Trypanosoma brucei and found evidences for its expression as a specific RNA class. The trypanosomatid TERRA are heterogeneous in size and partially polyadenylated. The levels of TERRA transcripts appear to be modulated through the life cycle in both trypanosomatids investigated, suggesting that TERRA play a stage-specific role in the life cycle of these early-branching eukaryotes...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28767982/description-of-an-oral-chagas-disease-outbreak-in-venezuela-including-a-vertically-transmitted-case
#4
Belkisyolé Alarcón de Noya, Gladymar Pérez-Chacón, Zoraida Díaz-Bello, Sonia Dickson, Arturo Muñoz-Calderón, Carlos Hernández, Yadira Pérez, Luciano Mauriello, Eyleen Moronta
We describe the eleventh major outbreak of foodborne Trypanosoma cruzi transmission in urban Venezuela, including evidence for vertical transmission from the index case to her fetus. After confirming fetal death at 24 weeks of gestation, pregnancy interruption was performed. On direct examination of the amniotic fluid, trypomastigotes were detected. T. cruzi specific-polymerase chain reaction (PCR) also proved positive when examining autopsied fetal organs. Finally, microscopic fetal heart examination revealed amastigote nests...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28767981/protection-mediated-by-chemokine-cxcl10-in-balb-c-mice-infected-by-leishmania-infantum
#5
Webertty Mayk Eufrásio Figueiredo, Sayonara de Melo Viana, Dorotheia Teixeira Alves, Priscila Valera Guerra, Zirlane Castelo Branco Coêlho, Helene Santos Barbosa, Maria Jania Teixeira
BACKGROUND: Visceral leishmaniasis (VL) caused by Leishmania infantum is characterised by the loss of the ability of the host to generate an effective immune response. Chemokines have a direct involvement in the pathogenesis of leishmaniasis, causing a rapid change in the expression of these molecules during infection by Leishmania. OBJECTIVES: Herein, it was investigated the role of CXCL10 in controlling infection by L. infantum. METHODS: RAW 264...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28767980/bone-marrow-cell-migration-to-the-heart-in-a-chimeric-mouse-model-of-acute-chagasic-disease
#6
Camila Iansen Irion, Bruno Diaz Paredes, Guilherme Visconde Brasil, Sandro Torrentes da Cunha, Luis Felipe Paula, Alysson Roncally Carvalho, Antonio Carlos Campos de Carvalho, Adriana Bastos Carvalho, Regina Coeli Dos Santos Goldenberg
BACKGROUND: Chagas disease is a public health problem caused by infection with the protozoan Trypanosoma cruzi. There is currently no effective therapy for Chagas disease. Although there is some evidence for the beneficial effect of bone marrow-derived cells in chagasic disease, the mechanisms underlying their effects in the heart are unknown. Reports have suggested that bone marrow cells are recruited to the chagasic heart; however, studies using chimeric mouse models of chagasic cardiomyopathy are rare...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28767979/risk-factors-for-hepatitis-b-transmission-in-south-brazil
#7
Vagner Reinaldo Zingalli Bueno Pereira, Jonas Michel Wolf, Camila Albani da Silva Luz, Gláucia Zuleide Stumm, Thais da Rocha Boeira, Josiane Galvan, Daniel Simon, Vagner Ricardo Lunge
BACKGROUND: Hepatitis B virus (HBV) infection is a major public health problem in Brazil. Several risk factors are involved in HBV infection and their identification by a rational and essential approach is required to prevent the transmission of this infection in Brazil. OBJECTIVES: To evaluate risk factors associated with HBV infection in South Brazil. METHODS: A total of 260 patients with HBV and 260 controls from Caxias do Sul (state of Rio Grande do Sul, Brazil) participated in this study...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28767978/biosynthesized-silver-nanoparticles-are-they-effective-antimicrobials
#8
Mudara K Peiris, Chinthika P Gunasekara, Pradeep M Jayaweera, Nuwan Dh Arachchi, Neluka Fernando
BACKGROUND: Silver nanoparticles (AgNPs) are increasingly being used in medical applications. Therefore, cost effective and green methods for generating AgNPs are required. OBJECTIVES: This study aimed towards the biosynthesis, characterisation, and determination of antimicrobial activity of AgNPs produced using Pseudomonas aeruginosa ATCC 27853. METHODS: Culture conditions (AgNO3 concentration, pH, and incubation temperature and time) were optimized to achieve maximum AgNP production...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28767977/clinical-and-serological-tests-for-arboviruses-in-free-living-domestic-pigeons-columba-livia
#9
Bruna Alves Ramos, Jannifer Oliveira Chiang, Lívia Carício Martins, Liliane Leal das Chagas, Franko de Arruda E Silva, Milene Silveira Ferreira, Maria Nazaré Oliveira Freitas, Bianca Nascimento de Alcantara, Sandro Patroca da Silva, Stefânia Araújo Miranda, Barbara Alves Sepulvreda, Layna Thayssa Guimarães Corrêa, Andréa Maria Góes Negrão, Pedro Fernando da Costa Vasconcelos, Alexandre do Rosário Casseb
BACKGROUND: In this study, we evaluated the role of free-living domestic pigeons (Columba livia) as a reservoir of arboviruses in the city of Belém, state of Pará, Brazil. We investigated the presence of antibodies against the most prevalent arboviruses. OBJECTIVES: This study was aimed at evaluating some clinical and physical parameters of domestic pigeons, including the presence of antibodies to Amazon-endemic arboviruses. METHODS: Eighty-five healthy pigeons were captured in Mangal das Garças Park, in Belém, and were bled...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28767976/chikungunya-virus-clinical-aspects-and-treatment-a-review
#10
Rivaldo V da Cunha, Karen S Trinta
Chikungunya is a severe and debilitating disease. Currently, Brazil is experiencing an epidemic caused by three arboviruses, which has changed the way health professionals have diagnosed and treated infected patients. The difficulty of diagnosis and the lack of a protocol for patient treatment, which fits Brazilian health system models, have made it difficult to manage this disease. It is necessary to implement a multidisciplinary network of patient care, in which primary care units play the main role. This review aims to present current information regarding the clinical aspects and treatment of Chikungunya virus infection...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591316/molecular-detection-of-mixed-infections-with-multiple-dengue-virus-serotypes-in-suspected-dengue-samples-in-tamaulipas-mexico
#11
LETTER
Rocío Requena-Castro, Miguel Ángel Reyes-López, Rosa Eminé Rodríguez-Reyna, Prisco Palma-Nicolás, Virgilio Bocanegra-García
This study aimed to detect dengue virus (DENV) serotypes in serum samples obtained in Matamoros Tamaulipas, Mexico, and to determine the concordance of conventional nested reverse transcriptase polymerase chain reaction (RT-PCR) and a serological test [enzyme-linked immunosorbent assay (ELISA NS1)]. Here, we detected mixed infections consisting of four serotypes of DENV. The most prevalent serotype was DENV-1, followed by DENV-4. This is the first report of DENV-4 in our region. Mixed infections were also detected in 21...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591315/trichophoromyia-auraensis-is-a-putative-vectorcomments-about-the-finding-of-trichophoromyia-auraensis-mangabeira-1942-sandfly-specimens-infected-by-leishmania-viannia-parasites-in-acre-statea-new-sandfly-in-the-subgenus-nyssomyia-diptera-psychodidae-from-the
#12
LETTER
Trichophoromyia auraensis is a putative vectorComments about the finding of Trichophoromyia auraensis (Mangabeira 1942) sandfly specimens infected by Leishmania (Viannia) parasites in Acre stateA new sandfly in the subgenus Nyssomyia (Diptera, Psychodidae) from the Amazon Basin of BrazilThe known geographical distribution of sandflies in the state of Acre, Brazil (Diptera: Psychodidae)Studies on the sandfly fauna (Diptera: Psychodidae: Phlebotominae) from transmission areas of American cutaneous leishmaniasis in state of Acre, BrazilDevelopment and validation of PCR-based assays for diagnosis of American cutaneous leishmaniasis and identification of the parasite speciesFirst description of Leishmania (Viannia) infection in Evandromyia saulensis, Pressatia sp. and Trichophoromyia auraensis (Psychodidae: Phlebotominae) in a transmission area of cutaneous leishmaniasis in Acre state, Amazon Basin, BrazilSand fly fauna (Diptera: Psychodidae: Phlebotominae) in an area of leishmaniasis transmission in the municipality of Rio Branco, state of Acre, BrazilIdentification of naturally infected Lutzomyia intermedia and Lutzomyia migonei with Leishmania (Viannia) braziliensis in Rio de Janeiro (Brazil) revealed by a PCR multiplex non-isotopic hybridization assayNotas sobre os flebotomíneos do estado do Acre, com a descrição de duas espécies novas (Diptera, Psychodidae)Description of Trichophoromyia ruifreitasi, a new phlebotomine species (Diptera, Psychodidae) from Acre state, Brazilian AmazonEpidemiologia da Leishmaniose Tegumentar e descrição das populações de flebotomíneos no município de Acrelândia, Acre, BrasilPhlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and BoliviaDescription of a new phlebotomine species (Diptera: Psychodidae, Phlebotominae) and new records of sand flies from the state of Acre, Northern BrazilNatural Leishmania infection of Lutzomyia (Trichophoromyia) auraensis in Madre de Dios, Peru, detected by a fluorescence resonance energy transfer-based real-time polymerase chain reactionGuide to the identification and geographic distribution of Lutzomyia sandflies in Mexico, the West Indies, Central and South America (Diptera: Psychodidae).
Carolina Bioni Garcia Teles, Felipe Arley Costa Pessoa, Jansen Fernandes Medeiros, Luís Marcelo Aranha Camargo
The sandfly Trichophoromyia auraensis has recently evolved as a proven vector of Leishmania (Viannia) endemic to state of Acre in the north of Brazil. This note is intended to propose a correction in the report of the first occurrence of natural infection of Leishmania (Viannia) in this species. We and the other scientific groups reinforced that Tr. auraensis is a possible vector involved in the transmission of American cutaneous leishmaniasis in Acre, Brazil.
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591314/complete-plasmid-sequence-carrying-type-iv-like-and-type-vii-secretion-systems-from-an-atypical-mycobacteria-strain
#13
Sergio Mascarenhas Morgado, Michel Abanto Marín, Fernanda S Freitas, Erica Lourenço Fonseca, Ana Carolina Paulo Vicente
The genus Mycobacterium is highly diverse and ubiquitous in nature, comprehending fast- and slow-growing species with distinct impact in public health. The plasmid-mediated horizontal gene transfer represents one of the major events in bacteria evolution. Here, we report the complete sequence of a 160,489 bp circular plasmid (pCBMA213_2) from an atypical and fast-growing environmental mycobacteria. This is a unique plasmid, in comparison with the characterised mycobacteria plasmids, harboring a type IV-like and ESX-P2 type VII secretion systems...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591313/multiplexed-reverse-transcription-real-time-polymerase-chain-reaction-for-simultaneous-detection-of-mayaro-oropouche-and-oropouche-like-viruses
#14
Felipe Gomes Naveca, Valdinete Alves do Nascimento, Victor Costa de Souza, Bruno Tardelli Diniz Nunes, Daniela Sueli Guerreiro Rodrigues, Pedro Fernando da Costa Vasconcelos
We describe a sensitive method for simultaneous detection of Oropouche and Oropouche-like viruses carrying the Oropouche S segment, as well as the Mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (RT-qPCR). A chimeric plasmid containing both Mayaro and Oropouche targets was designed and evaluated for the in vitro production of transcribed RNA, which could be easily used as a non-infectious external control. To track false-negative results due to PCR inhibition or equipment malfunction, the MS2 bacteriophage was also included in the multiplex assay as an internal positive control...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591312/identification-of-a-type-i-nitroreductase-gene-in-non-virulent-trypanosoma-rangeli
#15
Marjorie Montenegro, Claudia Cuervo, Constanza Cardenas, Silvia Duarte, Jenny R Díaz, M Carmen Thomas, Manuel C Lopez, Concepcion J Puerta
Trypanosomatid type I nitroreductases (NTRs), i.e., mitochondrial enzymes that metabolise nitroaromatic pro-drugs, are essential for parasite growth, infection, and survival. Here, a type I NTR of non-virulent protozoan Trypanosoma rangeli is described and compared to those of other trypanosomatids. The NTR gene was isolated from KP1(+) and KP1(-) strains, and its corresponding transcript and 5' untranslated region (5'UTR) were determined. Bioinformatics analyses and nitro-drug activation assays were also performed...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591311/participation-of-the-arcracme-protein-in-self-activation-of-the-arc-operon-located-in-the-arginine-catabolism-mobile-element-in-pandemic-clone-usa300
#16
Zayda Lorena Corredor Rozo, Ricaurte Alejandro Márquez-Ortiz, Betsy Esperanza Castro, Natasha Vanegas Gómez, Javier Escobar-Pérez
Staphylococcus aureus pandemic clone USA300 has, in addition to its constitutive arginine catabolism (arc) gene cluster, an arginine catabolism mobile element (ACME) carrying another such cluster, which gives this clone advantages in colonisation and infection. Gene arcR, which encodes an oxygen-sensitive transcriptional regulator, is inside ACME and downstream of the constitutive arc gene cluster, and this situation may have an impact on its activation. Different relative expression behaviours are proven here for arcRACME and the arcACME operon compared to the constitutive ones...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591310/human-papillomavirus-type-distribution-and-hpv16-intratype-diversity-in-southern-brazil-in-women-with-and-without-cervical-lesions
#17
Gisele R de Oliveira, Valdimara C Vieira, Emiliana C Ávila, Fabiana Finger-Jardim, Thaís Dm Caldeira, Fabiane Aa Gatti, Carla V Gonçalves, Sandro G Oliveira, Vanusa P da Hora, Marcelo A Soares, Ana Mb de Martinez
BACKGROUND: Increasing evidence suggests that human papillomavirus (HPV) intratype variants (specific lineages and sublineages) are associated with pathogenesis and progression from HPV infection to persistence and the development of cervical cancer. OBJECTIVES: This study aimed to verify the prevalence of HPV infection and distribution of HPV types and HPV16 variants in southern Brazil in women with normal cytology or intraepithelial lesions. METHODS: HPV typing was determined by L1 gene sequencing...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591309/detection-and-molecular-characterisation-of-a-diagnosis-escape-variant-associated-with-occult-hepatitis-b-virus-in-brazil
#18
Ricardo Wagner de Almeida, Francisco Campello do Amaral Mello, Isabelle Vasconcelos Menegoy, Márcia Paschoal do Espírito Santo, Cléber Ferreira Ginuíno, Paulo Sérgio Fonseca de Sousa, Livia Melo Villar, Elisabeth Lampe, Lia Laura Lewis-Ximenez
BACKGROUND: Many studies have identified mutations in the hepatitis B surface antigen (HBsAg) as important factors limiting the ability of commercial serological assays to detect this viral antigen. However, an association between mutations in the HBsAg gene and the occurrence of occult HBV infection (OBI) in patients has not been established. OBJECTIVES: To detect hepatitis B virus (HBV) DNA in patients with anti-HBc as a unique serological marker, a previously published, cost-effective TaqMan-based real-time polymerase chain reaction (PCR) test with minor groove binding probes was adapted for use in this study...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591308/prevalence-of-tuberculosis-respiratory-symptoms-and-associated-factors-in-the-indigenous-populations-of-paraguay-2012
#19
Sarita Aguirre, Celia Martínez Cuellar, María Belén Herrero, Gustavo Chamorro Cortesi, Nilda Gimenez de Romero, Mirian Alvarez, Jose Ueleres Braga
BACKGROUND: The prevalence of respiratory symptoms and confirmed tuberculosis (TB) among indigenous groups in Paraguay is unknown. METHODS: This study assessed the prevalence of respiratory symptoms, confirmed pulmonary TB, and associated socio-economic factors among indigenous Paraguayan populations. Indigenous persons residing in selected communities were included in the study. A total of 24,352 participants were interviewed at home between October and December 2012...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591307/schistosomiasis-and-hepatopulmonary-syndrome-the-role-of-concomitant-liver-cirrhosis
#20
Liana Gonçalves-Macedo, Edmundo Pessoa Lopes, Ana Lucia Coutinho Domingues, Brivaldo Markman, Vitor Gomes Mota, Carlos Feitosa Luna
BACKGROUND: Hepatopulmonary syndrome (HPS) is defined as an oxygenation defect induced by intrapulmonary vasodilation in patients with liver disease or portal hypertension. It is investigated in patients with liver cirrhosis and less frequently in those with portal hypertension without liver cirrhosis, as may occur in hepatosplenic schistosomiasis (HSS). OBJECTIVES: To investigate the prevalence of HPS in patients with HSS, and to determine whether the occurrence of HPS is influenced by concomitant cirrhosis...
July 2017: Memórias do Instituto Oswaldo Cruz
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